GapMind for catabolism of small carbon sources

 

Protein Ac3H11_2257 in Acidovorax sp. GW101-3H11

Annotation: FitnessBrowser__acidovorax_3H11:Ac3H11_2257

Length: 267 amino acids

Source: acidovorax_3H11 in FitnessBrowser

Candidate for 17 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-sorbitol (glucitol) catabolism sdh med L-iditol 2-dehydrogenase (EC 1.1.1.14) (characterized) 43% 95% 163.3 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
L-rhamnose catabolism LRA1 med L-rhamnose-1-dehydrogenase ( EC 1.1.1.173) (characterized) 41% 96% 151.8 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
glycerol catabolism dhaD lo NAD-dependent glycerol dehydrogenase; Dha-forming NAD-dependent glycerol dehydrogenase; EC 1.1.1.6 (characterized) 37% 96% 161 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
L-arabinose catabolism xacB lo L-arabinose 1-dehydrogenase (EC 1.1.1.46) (characterized) 36% 97% 156.8 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-galactose catabolism galdh lo L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized) 35% 97% 154.8 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
lactose catabolism galdh lo L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized) 35% 97% 154.8 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
L-fucose catabolism fucDH lo Short-chain dehydrogenase/reductase SDR (characterized, see rationale) 35% 97% 148.3 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
xylitol catabolism xdhA lo D-xylulose reductase (EC 1.1.1.9) (characterized) 38% 98% 141.4 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-xylose catabolism xdh lo D-xylose 1-dehydrogenase (EC 1.1.1.175) (characterized) 35% 99% 141.4 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-xylose catabolism xdhA lo D-xylulose reductase (EC 1.1.1.9) (characterized) 38% 98% 141.4 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
4-hydroxybenzoate catabolism badH lo BadH (characterized) 34% 98% 138.7 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
phenylacetate catabolism badH lo BadH (characterized) 34% 98% 138.7 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
L-phenylalanine catabolism badH lo BadH (characterized) 34% 98% 138.7 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-xylose catabolism DKDP-dehydrog lo SDR family oxidoreductase (characterized, see rationale) 34% 96% 136 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
L-rhamnose catabolism LRA5 lo 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; L-KDR 4-dehydrogenase; EC 1.1.1.401 (characterized) 35% 100% 132.1 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-sorbitol (glucitol) catabolism srlD lo Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (characterized) 32% 96% 122.5 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3
D-mannitol catabolism mt2d lo Probable NADP-dependent mannitol dehydrogenase; MtDH; Mannitol 2-dehydrogenase [NADP(+)]; Short chain dehydrogenase/reductase; YlSDR; EC 1.1.1.138 (characterized) 31% 93% 116.7 dihydroanticapsin dehydrogenase (EC 1.1.1.385) 42% 185.3

Sequence Analysis Tools

View Ac3H11_2257 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MNPSSPAPSLPSSIYFGHTGRVCIVTGGAQGIGEACVRRFAAEGAKPVIVDVDDARGQAL
AAELGALYVRCDVGDKAQVDALVAQVLAAHGRIDVLVNNAGIFRAADFLEVTEADFDAVL
RVNLKGSFLVGQAVARAMVTSGGGAIVNMSSVNGVLAIPNIASYNVSKGGVNQLTRVMAL
ALADKNIRVNAVAPGTIATELAAKAVLTSDEAKRKIMSRTPMKRLGQPSEIADVVAWLAS
DAASYVTGEIVTVDGGRMTLNYTVPVQ

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory