GapMind for catabolism of small carbon sources

 

Protein AO356_17540 in Pseudomonas fluorescens FW300-N2C3

Annotation: AO356_17540 PTS N-acetyl-D-glucosamine transporter

Length: 837 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 17 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
N-acetyl-D-glucosamine catabolism nagF hi N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized) 100% 100% 1627.1 D-trehalose PTS system, I, HPr, and IIA components 44% 646.4
D-glucosamine (chitosamine) catabolism nagF hi N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized) 100% 100% 1627.1 D-trehalose PTS system, I, HPr, and IIA components 44% 646.4
trehalose catabolism treEIIA med D-trehalose PTS system, I, HPr, and IIA components (characterized) 44% 98% 646.4 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-cellobiose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-glucose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
lactose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-maltose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
sucrose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
trehalose catabolism crr med PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component (characterized) 41% 86% 116.3 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
N-acetyl-D-glucosamine catabolism nagEIIA med Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) 42% 75% 112.5 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-glucosamine (chitosamine) catabolism nagEIIA med Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) 42% 75% 112.5 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-maltose catabolism malEIIA med Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) 42% 75% 112.5 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-fructose catabolism fruI lo Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized) 42% 70% 463.8 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
sucrose catabolism fruI lo Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized) 42% 70% 463.8 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
glycerol catabolism dhaM lo PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM; Dihydroxyacetone kinase subunit M; EC 2.7.1.121 (characterized) 32% 66% 124.8 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
N-acetyl-D-glucosamine catabolism crr lo Putative PTS system sugar phosphotransferase component IIA (characterized, see rationale) 36% 96% 85.9 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1
D-glucosamine (chitosamine) catabolism crr lo Putative PTS system sugar phosphotransferase component IIA (characterized, see rationale) 36% 96% 85.9 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) 100% 1627.1

Sequence Analysis Tools

View AO356_17540 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MPNNNKELILSAPLSGPVLTLANVPDAVFASGAMGDGIAIDPLNDTLYAPCDAEVIHVAR
TGHAVTLRADNGAELLLHLGLDTVELQGEGFSMLVKEGARVSHGQPLLRYDVDKVALRCK
SLVSLLVITNGEHFQARPITLKGVKVGEPLLHILAKAVGQAEHHDQDSGIEVFGQVRIAH
RGGLHARPAALVRQTAQGFKSRSQLHFSGKSASCDSVMGMMGLAITEQAQVHVSCRGSDA
EAALQALLTTLSTALVEEAHASAPPPEPPRANAEEGVLHGVCAAPGLVTGPLVRLSGIQL
PEDIGGHAIEEQRQRLSDALAQVRGEIHLTLEHARARQHRDEEAIFSAHLALLEDPVLLD
AADLFIEQGSAAPHAWSRSIDTQCQVLQQLGSTLLAERANDLRDLRQRVLRVLLGEAWQF
DVAAGAIVAAQELTPSDLLQLSAQGVAGVCMVEGGATSHVAILARGKGLPCLVALGDELL
AQEQGQAVVLDADGGRLELTPTVERLAQVRQAQTRRTALRAQQQSLAHTPARTVDGVEVE
VAANVASSAEAGESLANGADGVGLLRTEFLFVDRHTAPDEEEQRQAYQAVLEAMGDKPVI
IRTIDVGGDKQLDYLPLPSEANPVLGLRGIRLAQVRPDLLDQQLRALLQTRPLDRCRILL
PMVTEVDELLHIRKRLDALGSELGLSERPQLGVMVEVPAAALLAEQLAEHADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWVGVCGALASDPLATPVL
IGLGVRELSVSPPQIGEIKDRVRHLDAAQCARLSNELLNLGSALAVRRACHRHWPLG

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory