Protein WP_011382978.1 in Magnetospirillum magneticum AMB-1
Annotation: NCBI__GCF_000009985.1:WP_011382978.1
Length: 553 amino acids
Source: GCF_000009985.1 in NCBI
Candidate for 25 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-histidine catabolism | Ac3H11_2560 | med | ABC transporter for L-Histidine, ATPase component (characterized) | 46% | 89% | 208.8 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | med | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 41% | 81% | 166.8 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | med | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 41% | 81% | 166.8 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-cellobiose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-glucose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| lactose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-maltose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| sucrose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| trehalose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 37% | 86% | 171 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| L-fucose catabolism | SM_b21106 | lo | ABC transporter for L-Fucose, ATPase component (characterized) | 39% | 72% | 161 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| putrescine catabolism | potA | lo | PotG aka B0855, component of Putrescine porter (characterized) | 42% | 57% | 160.6 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-glucosamine (chitosamine) catabolism | SM_b21216 | lo | ABC transporter for D-Glucosamine, ATPase component (characterized) | 35% | 77% | 156.4 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-cellobiose catabolism | SMc04256 | lo | ABC transporter for D-Cellobiose and D-Salicin, ATPase component (characterized) | 38% | 68% | 152.5 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| trehalose catabolism | treV | lo | TreV, component of Trehalose porter (characterized) | 42% | 64% | 149.1 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-maltose catabolism | malK_Sm | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 36% | 60% | 144.1 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| trehalose catabolism | malK | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 36% | 60% | 144.1 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-mannitol catabolism | mtlK | lo | ABC transporter for D-Mannitol, D-Mannose, and D-Sorbitol, ATPase component (characterized) | 36% | 59% | 143.3 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-sorbitol (glucitol) catabolism | mtlK | lo | ABC transporter for D-Mannitol, D-Mannose, and D-Sorbitol, ATPase component (characterized) | 36% | 59% | 143.3 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| trehalose catabolism | thuK | lo | Trehalose import ATP-binding protein SugC; EC 7.5.2.- (characterized) | 31% | 90% | 142.5 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-maltose catabolism | malK | lo | Maltose-transporting ATPase (EC 3.6.3.19) (characterized) | 37% | 61% | 140.2 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| D-maltose catabolism | malK1 | lo | MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized) | 36% | 58% | 139.8 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| L-asparagine catabolism | peb1C | lo | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 37% | 88% | 130.6 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| L-aspartate catabolism | peb1C | lo | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 37% | 88% | 130.6 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| L-glutamate catabolism | gltL | lo | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 37% | 88% | 130.6 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
| glycerol catabolism | glpS | lo | GlpS, component of Glycerol uptake porter, GlpSTPQV (characterized) | 36% | 68% | 129.4 | ABC transporter for nitrate, ATPase component | 74% | 796.2 |
Sequence Analysis Tools
View WP_011382978.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MPILELKGVAKSYGASSVLRDIDLEIEDGEFIAILGFSGSGKTTLVSLMAGLIKPDAGEV
LLRGKPVDGPGADRGVVFQSYSLMPWLTVEGNIALAVDAVMPDASKAERKARVAKYIGMV
GLSHAAERRPSELSGGMRQRVAVARALAMSPDILLLDEPLSALDALTRAKLQDEIEAIWE
QEKKTVILITNDVDEALLLADRIIPLNPGPGATFGPSFKVNLPRPRDRAAVNSDPDFKRL
RAEVTEYLMAVGVERGGESGETRILPNVAPISFGGPPKAYREAGQVNNPDRYVEFSRVKK
IYPTPKGPLTVVDGFDLKMHQGEFISLIGHSGCGKSTVLTMTAGLTDVSEGGVILDGREV
SEAGPDRAVVFQAPSLFPWLTALQNVALGVDRVYPHASPAERLDIVSYYLERVGLGDSMD
KKASDMSNGMRQRVGIARAFALSPKLLLLDEPFGMLDSLTRWELQEVLMEVWTRTRVTAI
CVTHDVDEAILLADKVVMMTNGPNARIGKVLNVDIPRPRTRRALLEHPRYYEYRAEVLNF
LDEYETGAHAKAS
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory