Family Search for PF01170 (UPF0020)

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Family Search for PF01170 (UPF0020)

PF01170 hits 957 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

TRM14_METJA / Q57880 tRNA (guanine(6)-N2)-methyltransferase; tRNA m2G6-methyltransferase; EC 2.1.1.256 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q57880 tRNA (guanine6-N2)-methyltransferase (EC 2.1.1.256) from Methanocaldococcus jannaschii (see paper)
Aligns to 166:375 / 381 (55.1%), covers 99.5% of PF01170, 248.3 bits

function: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Cys).
catalytic activity: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine (RHEA:51116)

YPSC_BACSU / P50840 Putative RNA methyltransferase YpsC; EC 2.1.1.- from Bacillus subtilis (strain 168) (see paper)
Aligns to 164:370 / 385 (53.8%), covers 99.0% of PF01170, 234.5 bits

subunit: Interacts with the RNA polymerase core.

RlmK / b0948 fused 23S rRNA m²G2445 methyltransferase and 23S rRNA m⁷G2069 methyltransferase (EC 2.1.1.264; EC 2.1.1.173) from Escherichia coli K-12 substr. MG1655 (see 9 papers)
rlmL / P75864 fused 23S rRNA m²G2445 methyltransferase and 23S rRNA m⁷G2069 methyltransferase (EC 2.1.1.264; EC 2.1.1.173) from Escherichia coli (strain K12) (see 8 papers)
RLMKL_ECOLI / P75864 Ribosomal RNA large subunit methyltransferase K/L; EC 2.1.1.173; EC 2.1.1.264 from Escherichia coli (strain K12) (see 3 papers)
P75864 23S rRNA (guanine2445-N2)-methyltransferase (EC 2.1.1.173) from Escherichia coli (see 6 papers)
Aligns to 162:376 / 702 (30.6%), covers 99.5% of PF01170, 230.2 bits

function: Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits.
catalytic activity: guanosine(2445) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(2445) in 23S rRNA + S-adenosyl-L-homocysteine (RHEA:42740)
catalytic activity: guanosine(2069) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(2069) in 23S rRNA + S-adenosyl-L-homocysteine (RHEA:43772)
subunit: Monomer.

RLML_NEIMB / Q9K0V4 Ribosomal RNA large subunit methyltransferase L; 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase RlmL; EC 2.1.1.173 from Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58) (see paper)
Aligns to 165:370 / 380 (54.2%), covers 98.5% of PF01170, 220.9 bits

function: Specifically methylates the guanine in position 2445 (m2G2445) of 23S rRNA.
catalytic activity: guanosine(2445) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(2445) in 23S rRNA + S-adenosyl-L-homocysteine (RHEA:42740)

THUM3_MOUSE / P97770 tRNA (guanine(6)-N2)-methyltransferase THUMP3; GtROSA26asSor; THUMP domain-containing protein 3; EC 2.1.1.256 from Mus musculus (Mouse) (see 2 papers)
Aligns to 294:480 / 505 (37.0%), covers 99.0% of PF01170, 190.4 bits

function: Methyltransferase which catalyzes the formation of N(2)- methylguanosine at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs (By similarity). Also catalyzes the formation of N(2)-methylguanosine at position 7 of tRNA(Trp) (By similarity). Requires the methyltransferase adapter protein TRM112 for tRNA methyltransferase activity (By similarity).
catalytic activity: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine (RHEA:51116)
subunit: Interacts with TRMT112; the interaction is direct and is required for THUMPD3 methyltransferase activity.

THUM3_HUMAN / Q9BV44 tRNA (guanine(6)-N2)-methyltransferase THUMP3; THUMP domain-containing protein 3; EC 2.1.1.256 from Homo sapiens (Human) (see 2 papers)
Aligns to 292:478 / 507 (36.9%), covers 98.5% of PF01170, 170.5 bits

function: Methyltransferase which catalyzes the formation of N(2)- methylguanosine at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs (PubMed:34669960). Also catalyzes the formation of N(2)-methylguanosine at position 7 of tRNA(Trp) (PubMed:34669960). Requires the methyltransferase adapter protein TRM112 for tRNA methyltransferase activity (PubMed:34669960).
catalytic activity: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine (RHEA:51116)
subunit: Interacts with TRMT112; the interaction is direct and is required for THUMPD3 methyltransferase activity.

TRM14_PYRFU / Q8U248 tRNA (guanine(6)-N2)-methyltransferase; tRNA:m2G6 methyltransferase; EC 2.1.1.256 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 3 papers)
Q8U248 tRNA (guanine6-N2)-methyltransferase (EC 2.1.1.256) from Pyrococcus furiosus (see 2 papers)
Aligns to 191:360 / 365 (46.6%), covers 99.0% of PF01170, 138.1 bits

function: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).
catalytic activity: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine (RHEA:51116)
subunit: Monomer in solution.

PAB1283 / Q9UY84 tRNA (guanine¹⁰-N²)-dimethyltransferase (EC 2.1.1.213) from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
TMG10_PYRAB / Q9UY84 tRNA (guanine(10)-N2)-dimethyltransferase; (Pab)Trm-G10; tRNA:G10 dimethyltransferase; EC 2.1.1.213 from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
Q9UY84 tRNA (guanine10-N2)-dimethyltransferase (EC 2.1.1.213) from Pyrococcus abyssi (see paper)
Aligns to 154:322 / 329 (51.4%), covers 91.9% of PF01170, 102.2 bits

function: Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).
catalytic activity: guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine (RHEA:43124)
subunit: Monomer.

Q5JID5 tRNA (guanine10-N2)-methyltransferase (EC 2.1.1.214) from Thermococcus kodakarensis (see paper)
Aligns to 154:324 / 331 (51.7%), covers 85.8% of PF01170, 94.4 bits

THUM2_HUMAN / Q9BTF0 THUMP domain-containing protein 2 from Homo sapiens (Human) (see 2 papers)
Aligns to 272:433 / 503 (32.2%), covers 84.8% of PF01170, 93.7 bits

subunit: Interacts with TRMT112.

TRMN_THET2 / Q72IH5 tRNA (guanine(6)-N2)-methyltransferase; tRNA:m2G6 methyltransferase; EC 2.1.1.256 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 3 papers)
Q72IH5 tRNA (guanine6-N2)-methyltransferase (EC 2.1.1.256) from Thermus thermophilus (see 2 papers)
Aligns to 157:326 / 335 (50.7%), covers 86.3% of PF01170, 82.2 bits

function: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).
catalytic activity: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine (RHEA:51116)
subunit: Monomer in solution.
disruption phenotype: Inactivation of the gene leads to a total absence of m(2)G in tRNA but does not affect cell growth or the formation of other modified nucleosides in tRNA(Phe).

TRM11_HUMAN / Q7Z4G4 tRNA (guanine(10)-N2)-methyltransferase homolog; tRNA guanosine-2'-O-methyltransferase TRM11 homolog; EC 2.1.1.- from Homo sapiens (Human) (see paper)
Aligns to 189:326 / 463 (29.8%), covers 70.1% of PF01170, 45.9 bits

function: Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.
subunit: Interacts with TRMT112.

TRM11 / Q12463 tRNA (guanine¹⁰-N²)-monomethyltransferase catalytic subunit (EC 2.1.1.214) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see paper)
TRM11_YEAST / Q12463 tRNA (guanine(10)-N2)-methyltransferase; tRNA [Gm10] methyltransferase; tRNA guanosine-2'-O-methyltransferase TRM11; tRNA methylase 11; EC 2.1.1.214 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
Q12463 tRNA (guanine10-N2)-methyltransferase (EC 2.1.1.214) from Saccharomyces cerevisiae (see 3 papers)
Aligns to 183:303 / 433 (27.9%), covers 67.0% of PF01170, 43.8 bits

function: Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.
catalytic activity: guanosine(10) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine (RHEA:43128)
subunit: Interacts with TRM112.

MTV1_VIBS3 / Q03055 Type II methyltransferase M.VspI; M.VspI; Adenine-specific methyltransferase VspI; Modification methylase VspI; EC 2.1.1.72 from Vibrio sp. (strain 343) (see paper)
Aligns to 137:244 / 408 (26.5%), covers 56.3% of PF01170, 42.6 bits

function: A gamma subtype methylase, recognizes the double-stranded sequence 5'-ATTAAT-3', methylates A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.
catalytic activity: a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L- homocysteine (RHEA:15197)

Y3380_DICDI / Q54BW1 Putative uncharacterized protein DDB_G0293380 from Dictyostelium discoideum (Social amoeba) (see paper)
Aligns to 249:446 / 561 (35.3%), covers 55.3% of PF01170, 42.3 bits

ARSM_METAC / Q8TJK1 Arsenite methyltransferase; As(III) methyltransferase; EC 2.1.1.137 from Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) (see paper)
Aligns to 87:170 / 249 (33.7%), covers 26.4% of PF01170, 36.9 bits

function: Catalyzes the transfer of a methyl group from AdoMet to arsenite, producing methylated arsenicals. Involved in the conversion of As(III) to a number of methylated products. Reduces the arsenic toxicity in the cell and may contribute to the global arsenic cycling.
catalytic activity: [thioredoxin]-dithiol + arsenic triglutathione + 2 H2O + S- adenosyl-L-methionine = [thioredoxin]-disulfide + 3 glutathione + H(+) + methylarsonous acid + S-adenosyl-L-homocysteine (RHEA:69460)
catalytic activity: 2 [thioredoxin]-dithiol + arsenic triglutathione + H2O + 2 S- adenosyl-L-methionine = 2 [thioredoxin]-disulfide + dimethylarsinous acid + 3 glutathione + 2 H(+) + 2 S-adenosyl-L-homocysteine (RHEA:69464)
catalytic activity: 3 [thioredoxin]-dithiol + arsenic triglutathione + 3 S- adenosyl-L-methionine = 3 [thioredoxin]-disulfide + 3 glutathione + 3 H(+) + 3 S-adenosyl-L-homocysteine + trimethylarsine (RHEA:69432)

B9MK94 site-specific DNA-methyltransferase (cytosine-N4-specific) (EC 2.1.1.113) from Caldicellulosiruptor bescii (see paper)
Aligns to 29:100 / 416 (17.3%), covers 23.9% of PF01170, 36.5 bits

O93645 site-specific DNA-methyltransferase (cytosine-N4-specific) (EC 2.1.1.113) from Pyrococcus sp. (see paper)
Aligns to 41:126 / 433 (19.9%), covers 20.8% of PF01170, 35.2 bits

O52711 site-specific DNA-methyltransferase (cytosine-N4-specific) (EC 2.1.1.113) from Saccharopolyspora sp. (see paper)
Aligns to 35:142 / 376 (28.7%), covers 24.9% of PF01170, 34.8 bits

TRM11_SCHPO / O94636 tRNA (guanine(10)-N2)-methyltransferase; tRNA guanosine-2'-O-methyltransferase TRM11; tRNA methylase 11; EC 2.1.1.214 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
Aligns to 211:333 / 469 (26.2%), covers 69.0% of PF01170, 34.1 bits

function: Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.
catalytic activity: guanosine(10) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)- methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine (RHEA:43128)
subunit: Interacts with trm112.

METL5_CAEEL / Q18511 rRNA N6-adenosine-methyltransferase metl-5; Methyltransferase-like protein 5; EC 2.1.1.- from Caenorhabditis elegans (see paper)
Aligns to 27:141 / 214 (53.7%), covers 31.0% of PF01170, 33.0 bits

function: Catalytic subunit of a heterodimer with TRMT112/C04H5.1, which specifically methylates the 6th position of adenine in position 1717 of 18S rRNA.
catalytic activity: adenosine in rRNA + S-adenosyl-L-methionine = H(+) + N(6)- methyladenosine in rRNA + S-adenosyl-L-homocysteine (RHEA:58728)
subunit: Heterodimer; heterodimerizes with TRMT112/C04H5.1.
disruption phenotype: Worms are viable and do not display obvious developmental defects; they however exhibit significantly longer lifespan and heat resistance (PubMed:33357433). Cells show reduced N6- methylation of adenine(1717) in 18S rRNA (PubMed:33357433).

METL5_HUMAN / Q9NRN9 rRNA N6-adenosine-methyltransferase METTL5; Methyltransferase-like protein 5; EC 2.1.1.- from Homo sapiens (Human) (see 7 papers)
Aligns to 25:176 / 209 (72.7%), covers 55.8% of PF01170, 32.7 bits

function: Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA (PubMed:31328227, PubMed:32217665, PubMed:33357433, PubMed:33428944, PubMed:35033535). N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation (PubMed:33357433).
catalytic activity: adenosine(1832) in 18S rRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(1832) in 18S rRNA + S-adenosyl-L-homocysteine (RHEA:62612)
subunit: Heterodimer; heterodimerizes with TRMT112.

T4BA_HEYCO / Q07605 Type II restriction enzyme and methyltransferase RM.BcgI; Restriction enzyme BgcI subunit A; EC 3.1.21.4; EC 2.1.1.72 from Heyndrickxia coagulans (Weizmannia coagulans) (see 2 papers)
Aligns to 330:503 / 637 (27.3%), covers 87.8% of PF01170, 31.0 bits

function: A B, G, H and S subtype restriction enzyme that recognizes the double-stranded sequence 5'-CGAN(6)TGC-3' and cleaves bilaterally and symmetrically 10 base pairs upstream and 12 base pairs downstream of the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands; seems to methylate restricted DNA (PubMed:12654995, PubMed:8276869, PubMed:8451198). This subunit has no methylation or DNA restriction activity on its own (PubMed:8276869).
catalytic activity: Endonucleolytic cleavage of DNA to give specific double- stranded fragments with terminal 5'-phosphates.
catalytic activity: a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L- homocysteine (RHEA:15197)
cofactor: Mg(2+)
subunit: Heterotrimer of two A and one B subunit. Both subunits are necessary for DNA-binding, which is sequence non-specific.

Q7MPU6 type I site-specific deoxyribonuclease (EC 3.1.21.3) from Vibrio vulnificus (see paper)
Aligns to 205:320 / 530 (21.9%), covers 57.9% of PF01170, 30.8 bits

A0A0P0ETL9 ribosomal protein L3 N5-glutamine methyltransferase (EC 2.1.1.298) from Azospirillum brasilense (see paper)
Aligns to 138:245 / 313 (34.5%), covers 31.5% of PF01170, 30.7 bits

PRMC_THEMA / Q9WYV8 Release factor glutamine methyltransferase; RF MTase; N5-glutamine methyltransferase PrmC; Protein-(glutamine-N5) MTase PrmC; Protein-glutamine N-methyltransferase PrmC; EC 2.1.1.297 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
Q9WYV8 peptide chain release factor N5-glutamine methyltransferase (EC 2.1.1.297) from Thermotoga maritima (see 3 papers)
Aligns to 115:209 / 282 (33.7%), covers 29.9% of PF01170, 28.7 bits

function: Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.
catalytic activity: L-glutaminyl-[peptide chain release factor] + S-adenosyl-L- methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine (RHEA:42896)
subunit: Monomer and homodimer.

METL5_MOUSE / Q8K1A0 rRNA N6-adenosine-methyltransferase METTL5; Methyltransferase-like protein 5; EC 2.1.1.- from Mus musculus (Mouse) (see 4 papers)
Aligns to 25:171 / 209 (70.3%), covers 69.0% of PF01170, 28.3 bits

function: Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA (PubMed:32783360, PubMed:35033535). N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation (PubMed:32217665, PubMed:32783360).
catalytic activity: adenosine(1832) in 18S rRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(1832) in 18S rRNA + S-adenosyl-L-homocysteine (RHEA:62612)
subunit: Heterodimer; heterodimerizes with TRMT112.
disruption phenotype: Mice were born at non-Mendelian rates and develop morphological, such as craniofacial abnormalities, snout deviation due to altered nasal bone development and incomplete fusion of the frontal bone suture (PubMed:32217665). Mice display reduced body size and evidence of metabolic defects (PubMed:35033535). Mice also show behavioral abnormalities (PubMed:32217665). Deletion in embryonic stem cells (ESCs) results in a decrease in global translation rate, spontaneous loss of pluripotency and compromised differentiation potential (PubMed:32217665, PubMed:32783360). Cells show abolished level of N6-methylation of adenine(1832) in 18S rRNA (PubMed:35033535).

TrmM / b2575 tRNA m⁶A37 methyltransferase (EC 2.1.1.223) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
yfiC / P31825 tRNA m⁶A37 methyltransferase (EC 2.1.1.223) from Escherichia coli (strain K12) (see 3 papers)
TRMN6_ECOLI / P31825 tRNA1(Val) (adenine(37)-N6)-methyltransferase; tRNA m6A37 methyltransferase; EC 2.1.1.223 from Escherichia coli (strain K12) (see paper)
P31825 tRNA1Val (adenine37-N6)-methyltransferase (EC 2.1.1.223) from Escherichia coli (see paper)
Aligns to 27:187 / 245 (65.7%), covers 37.6% of PF01170, 26.9 bits

function: Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC).
catalytic activity: adenosine(37) in tRNA1(Val) + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(37) in tRNA1(Val) + S-adenosyl-L-homocysteine (RHEA:43160)
disruption phenotype: Cells lacking this gene show hypersensitivity to hyperosmotic and, to a lesser extent, oxidative stress.

TGS1_RAT / P85107 Trimethylguanosine synthase; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif; PIMT; PIPMT; EC 2.1.1.- from Rattus norvegicus (Rat) (see paper)
Aligns to 669:770 / 850 (12.0%), covers 52.3% of PF01170, 26.7 bits

function: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'- triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L- homocysteine (RHEA:78471)
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl- L-homocysteine (RHEA:78475)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78479)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78507)
subunit: May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).

TGS1_YEAST / Q12052 Trimethylguanosine synthase; Cap-specific guanine-N2 methyltransferase; snRNA/snoRNA cap hypermethylase; EC 2.1.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
Q12052 noranthrone synthase (EC 2.3.1.221) from Aspergillus sp. (see paper)
Aligns to 81:226 / 315 (46.3%), covers 52.3% of PF01170, 26.4 bits

function: Catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization.
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'- triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L- homocysteine (RHEA:78471)
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl- L-homocysteine (RHEA:78475)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78479)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78507)
subunit: Monomer (By similarity). Interacts with the spliceosomal snRNP core component SMB1 and the snoRNP components CBF5 and NOP58.

TGS1_HUMAN / Q96RS0 Trimethylguanosine synthase; CLL-associated antigen KW-2; Cap-specific guanine-N2 methyltransferase; Hepatocellular carcinoma-associated antigen 137; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif; PIMT; PIPMT; EC 2.1.1.- from Homo sapiens (Human) (see 8 papers)
Aligns to 676:769 / 853 (11.0%), covers 46.2% of PF01170, 26.1 bits

function: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'- triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L- homocysteine (RHEA:78471)
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl- L-homocysteine (RHEA:78475)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78479)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78507)
subunit: May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.

TGS1_MOUSE / Q923W1 Trimethylguanosine synthase; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein with methyltransferase motif; PIMT; PIPMT; EC 2.1.1.- from Mus musculus (Mouse) (see paper)
Aligns to 667:761 / 853 (11.1%), covers 52.3% of PF01170, 26.1 bits

function: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation (By similarity).
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'- triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L- homocysteine (RHEA:78471)
catalytic activity: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl- L-homocysteine (RHEA:78475)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78479)
catalytic activity: a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)- ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + H(+) + S-adenosyl-L-homocysteine (RHEA:78507)
subunit: May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN (By similarity).

METL5_DANRE / F1QVR8 rRNA N6-adenosine-methyltransferase METTL5; Methyltransferase-like protein 5; EC 2.1.1.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
Aligns to 27:157 / 207 (63.3%), covers 36.0% of PF01170, 25.2 bits

function: Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA (By similarity). N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation (By similarity).
catalytic activity: adenosine(1832) in 18S rRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(1832) in 18S rRNA + S-adenosyl-L-homocysteine (RHEA:62612)
disruption phenotype: Microcephaly and curved tails.

arsM / Q6N3Y0 arsenite methyltransferase (EC 2.1.1.137) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see 3 papers)
ARSM_RHOPA / Q6N3Y0 Arsenite methyltransferase; As(III) AdoMet methyltransferase; EC 2.1.1.137 from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see paper)
Aligns to 91:154 / 283 (22.6%), covers 26.9% of PF01170, 24.3 bits

function: Catalyzes the transfer of a methyl group from AdoMet to arsenite, producing methylated arsenicals. Involved in the conversion of As(III) to a number of di- and trimethylated species, with trimethylarsine as the end product. Reduces the arsenic toxicity in the cell and may contribute to the global arsenic cycling.
catalytic activity: [thioredoxin]-dithiol + arsenic triglutathione + 2 H2O + S- adenosyl-L-methionine = [thioredoxin]-disulfide + 3 glutathione + H(+) + methylarsonous acid + S-adenosyl-L-homocysteine (RHEA:69460)
catalytic activity: 2 [thioredoxin]-dithiol + arsenic triglutathione + H2O + 2 S- adenosyl-L-methionine = 2 [thioredoxin]-disulfide + dimethylarsinous acid + 3 glutathione + 2 H(+) + 2 S-adenosyl-L-homocysteine (RHEA:69464)
catalytic activity: 3 [thioredoxin]-dithiol + arsenic triglutathione + 3 S- adenosyl-L-methionine = 3 [thioredoxin]-disulfide + 3 glutathione + 3 H(+) + 3 S-adenosyl-L-homocysteine + trimethylarsine (RHEA:69432)

YABB_BACSU / P37543 Probable RNA methyltransferase YabB from Bacillus subtilis (strain 168) (see paper)
Aligns to 59:196 / 247 (55.9%), covers 35.0% of PF01170, 23.6 bits

function: Methylates cellular RNA.
disruption phenotype: No visible growth defects, DNA is still methylated on A-5 of 5'-GACGAG-3'.

Or search for genetic data about PF01170 in the Fitness Browser

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory