Family Search for PF07063 (DUF1338)

PF07063 hits 32 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

SMc04383 putative hydrolase, required for lysine catabolism from Sinorhizobium meliloti 1021
Aligns to 197:412 / 466 (46.4%), covers 99.4% of PF07063, 227.7 bits

• mutant phenotype: PFam PF07063.9 (DUF1338). This gene is specifically important for utilizing lysine, and this phenotype is conserved. It might be the decarboxylating 2-oxoadipate hydrolase of the D-lysine oxidase pathway, which has not been linked to a gene, or the crotonyl-CoA hydratase in the glutaryl-CoA degradation pathway.

PP_5260 2-oxoadipate decarboxylase/hydroxylase (2-hydroxyglutarate synthase) from Pseudomonas putida KT2440
ydcJ / Q88CC1 2-oxoadipate dioxygenase/decarboxylase (EC 1.13.11.93) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 2 papers)
HGLS_PSEPK / Q88CC1 2-oxoadipate dioxygenase/decarboxylase; 2-hydroxyglutarate synthase; EC 1.13.11.93 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 2 papers)
Aligns to 197:412 / 464 (46.6%), covers 99.4% of PF07063, 225.8 bits

• mutant phenotype: Specifically important in carbon source L-Lysine; carbon source D-Lysine. This is part of the pathway from D-lysine to 2-oxoglutarate. For biochemical evidence that this enzyme forms 2-hydroxyglutarate, see https://doi.org/10.1101/450254
• function: Catalyzes the decarboxylation and hydroxylation of 2- oxoadipate (2OA) to form D-2-hydroxyglutarate (D-2-HGA) (PubMed:31064836, PubMed:32523014). Is specific for 2-oxoadipate (PubMed:32523014). Is involved in a D-lysine catabolic pathway (PubMed:31064836).
  catalytic activity: 2-oxoadipate + O2 = (R)-2-hydroxyglutarate + CO2 (RHEA:71787)
  cofactor: Fe(2+)
  disruption phenotype: Deletion mutant is unable to grow on either isomer of lysine.

AO356_01105 putative hydrolase, required for lysine catabolism from Pseudomonas fluorescens FW300-N2C3
Aligns to 194:409 / 458 (47.2%), covers 99.4% of PF07063, 224.0 bits

• mutant phenotype: PFam PF07063.9 (DUF1338). This gene is specifically important for utilizing lysine, and this phenotype is conserved. It might be the decarboxylating 2-oxoadipate hydrolase of the D-lysine oxidase pathway, which has not been linked to a gene, or the crotonyl-CoA hydratase in the glutaryl-CoA degradation pathway.

YdcJ / b1423 DUF1338 domain-containing protein YdcJ from Escherichia coli K-12 substr. MG1655 (see paper)
HGLS_ECOLI / P76097 2-oxoadipate dioxygenase/decarboxylase; 2-hydroxyglutarate synthase; EC 1.13.11.93 from Escherichia coli (strain K12) (see paper)
Aligns to 195:401 / 447 (46.3%), covers 99.4% of PF07063, 221.1 bits

• function: Catalyzes the decarboxylation and hydroxylation of 2- oxoadipate (2OA) to form D-2-hydroxyglutarate (D-2-HGA).
  catalytic activity: 2-oxoadipate + O2 = (R)-2-hydroxyglutarate + CO2 (RHEA:71787)
  cofactor: Fe(2+)

HGLS_ARATH / Q9LMJ7 2-oxoadipate dioxygenase/decarboxylase, chloroplastic; 2-hydroxyglutarate synthase; EC 1.13.11.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Aligns to 223:362 / 371 (37.7%), covers 97.0% of PF07063, 113.6 bits

• function: Catalyzes the decarboxylation and hydroxylation of 2- oxoadipate (2OA) to form D-2-hydroxyglutarate (D-2-HGA) (PubMed:32523014). Is involved in a D-lysine catabolic pathway (PubMed:32523014).
  catalytic activity: 2-oxoadipate + O2 = (R)-2-hydroxyglutarate + CO2 (RHEA:71787)
  cofactor: Fe(2+)
  disruption phenotype: Delayed germination, compromised development, cholortic phenotype with impaired growth, due to lysine accumulation.

HGLS_ORYSJ / Q337M4 2-oxoadipate dioxygenase/decarboxylase, chloroplastic/amyloplastic; 2-hydroxyglutarate synthase; Protein FLOURY ENDOSPERM 7; EC 1.13.11.93 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
Aligns to 216:355 / 364 (38.5%), covers 97.6% of PF07063, 107.8 bits

• function: Catalyzes the decarboxylation and hydroxylation of 2- oxoadipate (2OA) to form D-2-hydroxyglutarate (D-2-HGA) (PubMed:32523014). Is involved in a D-lysine catabolic pathway (PubMed:32523014). Involved in the regulation of starch synthesis and amyloplast development within the peripheral endosperm during the grain-filling stage (PubMed:26608643).
  catalytic activity: 2-oxoadipate + O2 = (R)-2-hydroxyglutarate + CO2 (RHEA:71787)
  cofactor: Fe(2+)
  disruption phenotype: Reduced grain filling rate, reduced grain weight, reduced seed thickness, opaque periphery of grain endosperm and defect in starch accumulation in the periphery of the endosperm during seed development, due to lysine accumulation.

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