Family Search for PF10350 (DUF2428)

PF10350 hits 11 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

THADA_HUMAN / Q6YHU6 tRNA (32-2'-O)-methyltransferase regulator THADA; Gene inducing thyroid adenomas protein; Thyroid adenoma-associated protein from Homo sapiens (Human) (see 2 papers)
Aligns to 947:1248 / 1953 (15.5%), covers 99.6% of PF10350, 286.4 bits

• function: Together with methyltransferase FTSJ1, methylates the 2'-O- ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.

THADA_DROME / Q9VWB9 tRNA (32-2'-O)-methyltransferase regulator THADA; Thyroid adenoma-associated protein homolog from Drosophila melanogaster (Fruit fly) (see 2 papers)
Aligns to 838:1113 / 1746 (15.8%), covers 97.3% of PF10350, 259.5 bits

• function: Together with methyltransferase Trm7-32, methylates the 2'-O- ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs (By similarity). Plays a key role in energy homeostasis by regulating the balance between energy storage and heat production. Functions by negatively regulating Ca(2+) signaling pathways that are involved in heat production and maintaining correct lipid storage in the fat body. Regulates Ca(2+) signaling pathways by reducing the activity of the calcium-transporting ATPase SERCA possibly by promoting uncoupling of SERCA ATP hydrolysis from calcium pumping. May also function in the nervous system to control feeding behavior (PubMed:28399403).
  subunit: Interacts with SERCA.
  disruption phenotype: Flies are hypersensitive to the cold, hyperphagic and display elevated triglyceride stores resulting in an increased resistance to starvation (PubMed:28399403). Heat production is impaired and flies are slow to recover after cold treatment (PubMed:28399403). The size of the lipid droplets in larval fat bodies are significantly increased and flies display increased feeding (PubMed:28399403). Total body glycogen is also significantly increased in adult females but not in males (PubMed:28399403). Mutants also display an increase in calcium-dependent SERCA activity (PubMed:28399403). No effect on levels of circulating sugars, glucose and trehalose (PubMed:28399403). RNAi- mediated knockdown impairs small interfering RNA-mediated silencing (PubMed:31943105).

THADA_SCHPO / O60081 tRNA (32-2'-O)-methyltransferase regulator trm732 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
Aligns to 624:868 / 1502 (16.3%), covers 99.2% of PF10350, 244.6 bits

• function: Together with methyltransferase trm7, methylates the 2'-O- ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.
  disruption phenotype: Loss of methylation of the 2'-O-ribose of cytidine at position 32 of tRNA(Phe) (PubMed:25404562). Increases occurrence of 1-methylguanosine residue at position 37 in the tRNA anticodon loop of tRNA(Phe) and decreases occurrence of wybutosine at this position (PubMed:25404562).

THADA_YEAST / Q03496 tRNA (32-2'-O)-methyltransferase regulator TRM732 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
Aligns to 614:839 / 1420 (15.9%), covers 99.6% of PF10350, 177.2 bits

• function: Together with methyltransferase TRM7, methylates the 2'-O- ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.
  subunit: Interacts with TRM7; for 2'-O-methylation of position 32 in substrate tRNAs.
  disruption phenotype: Loss of methylation of the 2'-O-ribose of cytidine at position 32 of tRNA(Phe) (PubMed:35559166). Increases occurrence of 1-methylguanosine residue at position 37 tRNA anticodon loop of tRNA(Phe), indicative of a loss of wybutosine at this position (PubMed:35559166). Simultaneous knockout of RTT10/TRM734 leads to activation of the general control amino acid (GAAC) response and severely decreases cell population growth (PubMed:29596413, PubMed:35559166).

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