Family Search for PF14574 (RACo_C_ter)

PF14574 hits 40 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

PGA1_c15200 ATP-dependent reduction of co(II)balamin (RamA-like) (EC:2.1.1.13) from Phaeobacter inhibens BS107
Aligns to 395:668 / 698 (39.3%), covers 98.5% of PF14574, 292.0 bits

• mutant phenotype: Apparently required for the reactivation of vitamin B12. Distantly related to RamA (see PMID: 19043046) (auxotroph)

B8R2M5 [Co(II) methylated amine-specific corrinoid protein] reductase (EC 1.16.99.1) from Acetobacterium dehalogenans (see paper)
Aligns to 339:598 / 598 (43.5%), covers 96.9% of PF14574, 251.6 bits

RAMQ_EUBLI / P0DX10 Corrinoid activation enzyme RamQ from Eubacterium limosum (see 2 papers)
Aligns to 334:592 / 593 (43.7%), covers 95.0% of PF14574, 245.8 bits

• function: Involved in the degradation of the quaternary amines L- proline betaine and L-carnitine (PubMed:31341018, PubMed:32571881). Component of a corrinoid-dependent methyltransferase system that transfers a methyl group from L-proline betaine or L-carnitine to tetrahydrofolate (THF), forming methyl-THF, a key intermediate in the Wood-Ljungdahl acetogenesis pathway (PubMed:31341018, PubMed:32571881). RamQ is not required for the methyl transfer, but it stimulates reduction of reconstituted MtqC from the Co(II) state to the Co(I) state in vitro (PubMed:31341018). It also stimulates the rate of THF methylation (PubMed:32571881).
  cofactor: [2Fe-2S] cluster (Binds 1 2Fe-2S cluster.)

ramA / B8Y445 [Co(II) methylated amines-specific corrinoid protein] reductase (EC 1.16.99.1) from Methanosarcina barkeri (see 2 papers)
RAMA_METBA / B8Y445 [Co(II) methylated amine-specific corrinoid protein] reductase; Corrinoid activation enzyme RamA; EC 1.16.99.1 from Methanosarcina barkeri (see paper)
B8Y445 [Co(II) methylated amine-specific corrinoid protein] reductase (EC 1.16.99.1) from Methanosarcina barkeri (see paper)
Aligns to 168:427 / 540 (48.1%), covers 98.5% of PF14574, 209.8 bits

• function: Reductase required for the activation of corrinoid-dependent methylamine methyltransferase reactions during methanogenesis (PubMed:19043046). Mediates the ATP-dependent reduction of corrinoid proteins from the inactive cobalt(II) state to the active cobalt(I) state (PubMed:19043046). Acts on the corrinoid proteins involved in methanogenesis from monomethylamine (MMA), dimethylamine (DMA) and trimethylamine (TMA), namely MtmC, MtbC and MttC, respectively (PubMed:19043046).
  catalytic activity: AH2 + ATP + 2 Co(II)-[methylamine-specific corrinoid protein] + H2O = A + ADP + 2 Co(I)-[methylamine-specific corrinoid protein] + 3 H(+) + phosphate (RHEA:65816)
  catalytic activity: AH2 + ATP + 2 Co(II)-[dimethylamine-specific corrinoid protein] + H2O = A + ADP + 2 Co(I)-[dimethylamine-specific corrinoid protein] + 3 H(+) + phosphate (RHEA:65832)
  catalytic activity: AH2 + ATP + 2 Co(II)-[trimethylamine-specific corrinoid protein] + H2O = A + ADP + 2 Co(I)-[trimethylamine-specific corrinoid protein] + 3 H(+) + phosphate (RHEA:65836)
  cofactor: [4Fe-4S] cluster (Binds 2 [4Fe-4S] clusters.)
  subunit: Monomer.

DvMF_1398 ATP-dependent reduction of co(II)balamin (RamA-like) from Desulfovibrio vulgaris Miyazaki F
Aligns to 434:685 / 685 (36.8%), covers 93.9% of PF14574, 143.7 bits

• mutant phenotype: Cofit with the B12-dependent methionine synthase (DvMF_0476), which lacks a standard domain for the reactivation of vitamin B12.

DVU0908 ATP-dependent reduction of co(II)balamin from Desulfovibrio vulgaris Hildenborough JW710
Aligns to 296:542 / 543 (45.5%), covers 94.3% of PF14574, 139.1 bits

• mutant phenotype: Important for fitness in most defined media. Semi-automated annotation based on the auxotrophic phenotype and a hit to HMM PF14574.

Or search for genetic data about PF14574 in the Fitness Browser