Family Search for PF13826 (DUF4188)

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PF13826 hits 5 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

fmaE / Q4WAZ2 prefumagillin monooxygenase from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (see 3 papers)
FMAE_ASPFU / Q4WAZ2 Monooxygenase af470; Fumagillin biosynthesis antibiotic biosynthesis monooxygenase superfamily monooxygenase; Fma-ABM; EC 1.13.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see 8 papers)
Aligns to 113:235 / 275 (44.7%), covers 100.0% of PF13826, 114.8 bits

• function: Monooxygenase; part of the gene cluster that mediates the biosynthesis of fumagillin, a meroterpenoid that has numerous biological activities including irreversible inhibition of human type 2 methionine aminopeptidase (METAP2) (PubMed:23488861, PubMed:24568283). Within the pathway, the monooxygenase af470 catalyzes the oxidative cleavage of prefumagillin to yield the final compound of the pathway, fumagillin (PubMed:24568283). The pathway begins with the conversion of farnesyl pyrophosphate (FPP) to beta-trans-bergamotene by the membrane- bound beta-trans-bergamotene synthase af520. The multifunctional cytochrome P450 monooxygenase af510 then converts beta-trans- bergamotene into 5-keto-demethoxyfumagillol via several oxydation steps. 5-keto-demethoxyfumagillol is then subjected to successive C-6 hydroxylation and O-methylation by the dioxygenase af480 and O- methyltransferase af390-400, respectively, to yield 5-keto-fumagillol, which is then stereoselectively reduced by the keto-reductase af490 to 5R-hydroxy-seco-sesquiterpene. The next step is the polyketide transferase af380-catalyzed transfer of a dodecapentaenoyl group synthesized by the polyketide synthase af370 onto 5R-hydroxy-seco- sesquiterpene which leads to the production of prefumagillin. Finally, oxidative cleavage by the monooxygenase af470 converts prefumagillin to fumagillin (PubMed:24568283) (Probable).
  catalytic activity: NADPH + 2 O2 + prefumagillin = acetaldehyde + fumagillin + H2O + NADP(+) (RHEA:74627)
  disruption phenotype: Completely abolishes the production of fumagillin but leads to the accumulation of the intermediate prefumagillin (PubMed:24082142, PubMed:24568283).

oxdA / P82604 phenylacetaldoxime dehydratase (EC 4.8.1.4) from Bacillus sp. (strain OxB-1) (see paper)
OXD_BACSX / P82604 Phenylacetaldoxime dehydratase; EC 4.8.1.4 from Bacillus sp. (strain OxB-1) (see paper)
A0A0A8JIJ3 aliphatic aldoxime dehydratase (EC 4.8.1.2) from Bacillus sp. OxB-1 (see paper)
P82604 phenylacetaldoxime dehydratase (EC 4.8.1.4) from Bacillus sp. (in: Bacteria) (see 2 papers)
Aligns to 220:329 / 351 (31.3%), covers 68.6% of PF13826, 46.9 bits

• function: Catalyzes the stoichiometric dehydration of Z- phenylacetaldoxime to phenylacetonitrile. Prefers the Z-form of phenylacetaldoxime over its E-isomer.
  catalytic activity: (Z)-phenylacetaldehyde oxime = H2O + phenylacetonitrile (RHEA:20069)
  cofactor: heme b
  subunit: Monomer.

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