SitesHMM for TIGR00110

SitesBLAST – Find functional sites

SitesHMM for TIGR00110

Comparing TIGR00110 to proteins with known functional sites using HMMer

Or try Family Search vs. Papers

Found 4 hits to proteins with known functional sites (download)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
733.8 bits, 100% coverage: 2:542/543 of HMM aligns to 69:607/608 of Q9LIR4

HMM
sites
Q9LIR4

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C100 (≠ G33) binding [2Fe-2S] cluster
C173 (= C106) binding [2Fe-2S] cluster
D174 (= D107) binding Mg(2+)
C245 (= C179) binding [2Fe-2S] cluster
E497 (= E432) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
730.0 bits, 100% coverage: 2:542/543 of HMM aligns to 31:569/570 of 8hs0A

HMM
sites
8hs0A

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D

binding fe2/s2 (inorganic) cluster: C62 (≠ G33), D94 (= D65), A95 (≠ G66), C135 (= C106), D136 (= D107), C207 (= C179)
binding magnesium ion: D136 (= D107), K137 (= K108), N285 (= N257), H289 (= H261)

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
711.3 bits, 99% coverage: 2:540/543 of HMM aligns to 20:560/562 of 6ovtA

HMM
sites
6ovtA

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S

L

A

T

A

E

E

A

A

L

L

G

G

L

M

S

S

L

L

P

P

G

G

S

S

S

A

T

A

L

P

L

P

A

A

T

T

S

D

A

R

E

R

K

R

K

D

E

G

L

F

A

A

K

R

K

R

S

S

G

G

K

Q

R

A

I

V

V

V

E

E

L

L

V

L

K

R

K

R

N

G

I

I

K

T

P

A

R

R

D

D

I

I

L

L

T

T

K

K

E

E

A

A

F

F

E

E

N

N

A

A

I

I

T

A

L

V

D

V

L

M

A

A

L

F

G

G

G

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

L

L

L

L

A

A

I

I

A

A

K

H

E

E

A

A

G

N

V

V

K

A

L

L

S

S

L

L

D

Q

D

D

F

F

D

S

R

R

L

I

S

G

R

S

K

G

V

V

P

P

L

H

L

L

A

A

K

D

L

V

K

K

P

P

S

F

G

G

K

R

K

H

V

V

I

M

E

S

D

D

L

V

H

D

R

H

A

I

G

G

G

G

V

V

S

P

A

V

V

V

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M

K

K

E

A

L

L

D

L

K

D

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A

G

G

L

L

L

L

H

H

K

G

D

D

A

C

L

L

T

T

V

V

T

T

G

G

K

H

T

T

L

M

A

A

E

E

T

N

L

L

E

A

K

A

V

I

K

T

V

P

L

P

R

D

V

P

D

D

Q

G

D

K

V

V

I

L

R

R

S

A

L

L

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A

N

N

P

P

V

I

K

H

K

P

E

S

G

G

G

G

L

I

A

T

V

I

L

L

K

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G

N

S

L

L

A

A

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P

E

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G

A

A

V

V

V

V

K

K

I

T

A

A

G

G

V

F

E

D

E

S

D

-

I

-

L

D

K

V

F

F

E

E

G

G

P

T

A

A

K

R

V

V

F

F

E

D

S

G

E

E

E

R

E

A

A

A

L

L

E

D

A

A

I

L

L

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G

D

G

G

K

T

V

I

K

T

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D

D

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A

V

V

V

V

I

I

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R

Y

Y

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

R

R

E
|
E

M

M

L

L

A

A

P

I

T

T

S

G

A

A

L

I

V

K

G

G

L

A

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G

L

L

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K

K

D

V

V

A

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L

L

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L

T

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D

D

G

G

R

R

F

F

S

S

G

G

G

G

T

T

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T

G

G

L

L

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C

I

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

A

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E

D

G

G

G

G

A

P

I

I

A

A

L

L

V

L

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N

G

G

D

D

K

R

I

I

K

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I

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D

D

I

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D

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F

A

A

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R

R

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A

Q

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D

A

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K

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A

P

R

R

E

Y

V

T

K

T

G

G

A

V

L

L

A

S

K

K

Y

Y

A

V

K

K

L

L

V

V

S

S

S

S

A

A

D

A

K

V

G

G

A

A

V

V

binding fe2/s2 (inorganic) cluster: C51 (≠ G33), G84 (= G66), C124 (= C106), D125 (= D107), A200 (≠ S178), C201 (= C179)
binding magnesium ion: D83 (= D65), D125 (= D107), K126 (= K108), E452 (= E432)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
711.2 bits, 99% coverage: 2:540/543 of HMM aligns to 33:573/575 of P9WKJ5

HMM
sites
P9WKJ5

A

A

R

R

A

G

L

M

L

L

K

R

A

A

T

V

G

G

L

M

K

D

D

D

E

E

D

D

L

F

E

A

K

K

P

P

I

Q

I

I

A

G

V

V

V

A

N

S

S

S

Y

W

T

N

E

E

I

I

V

T

P

P

G
x
C

H

N

V

L

H

S

L

L

K

D

D

R

L

L

A

A

K

N

L

A

V

V

K

K

E

E

E

G

I

V

E

F

A

S

A

A

G

G

G

G

V

Y

A

P

K

L

E

E

F

F

N

G

T

T

I

I

A

S

V

V

S

S

D
|
D

G

G

I

I

A

S

M

M

G

G

H

H

E

E

G

G

M

M

K

H

Y

F

S

S

L

L

P

V

S

S

R

R

E

E

I

V

I

I

A

A

D

D

S

S

V

V

E

E

T

V

V

V

V

M

K

Q

A

A

H

E

A

R

L

L

D

D

A

G

L

S

V

V

V

L

I

L

S

A

S

G

C
|
C

D
|
D

K
|
K

I

S

V

L

P

P

G

G

M

M

L

L

M

M

A

A

A

A

L

A

R

R

L

L

N

D

I

L

P

A

A

A

I

V

V

F

V

L

S

Y

G

A

G

G

P

S

M

I

E

L

A

P

G

G

K

R

T

A

K

K

L

L

-

S

-

D

-

G

-

S

S

E

E

R

K

D

I

V

D

T

L

I

V

I

D

D

V

A

F

F

E

E

A

A

V

V

G

G

E

A

Y

C

A

S

A

R

G

G

K

L

L

M

S

S

E

R

E

A

E

D

L

V

E

D

E

A

I

I

E

E

R

R

S

A

A

I

C

C

P

P

T

G

A

E

G

G

S

A

C
|
C

S

G

G

G

L

M

F

Y

T

T

A

A

N

N

S

T

M

M

A

A

C

S

L

A

T

A

E

E

A

A

L

L

G

G

L

M

S

S

L

L

P

P

G

G

S

S

S

A

T

A

L

P

L

P

A

A

T

T

S

D

A

R

E

R

K

R

K

D

E

G

L

F

A

A

K

R

K

R

S

S

G

G

K

Q

R

A

I

V

V

V

E

E

L

L

V

L

K

R

K

R

N

G

I

I

K

T

P

A

R

R

D

D

I

I

L

L

T

T

K

K

E

E

A

A

F

F

E

E

N

N

A

A

I

I

T

A

L

V

D

V

L

M

A

A

L

F

G

G

G

G

S

S

T

T

N

N

T

A

V

V

L

L

H

H

L

L

L

L

A

A

I

I

A

A

K

H

E

E

A

A

G

N

V

V

K

A

L

L

S

S

L

L

D

Q

D

D

F

F

D

S

R

R

L

I

S

G

R

S

K

G

V

V

P

P

L

H

L

L

A

A

K

D

L

V

K

K

P

P

S

F

G

G

K

R

K

H

V

V

I

M

E

S

D

D

L

V

H

D

R

H

A

I

G

G

G

G

V

V

S

P

A

V

V

V

L

M

K

K

E

A

L

L

D

L

K

D

E

A

G

G

L

L

L

L

H

H

K

G

D

D

A

C

L

L

T

T

V

V

T

T

G

G

K

H

T

T

L

M

A

A

E

E

T

N

L

L

E

A

K

A

V

I

K

T

V

P

L

P

R

D

V

P

D

D

Q

G

D

K

V

V

I

L

R

R

S

A

L

L

D

A

N

N

P

P

V

I

K

H

K

P

E

S

G

G

G

G

L

I

A

T

V

I

L

L

K

H

G

G

N

S

L

L

A

A

E

P

E

E

G

G

A

A

V

V

V

V

K

K

I

T

A

A

G

G

V

F

E

D

E

S

D

-

I

-

L

D

K

V

F

F

E

E

G

G

P

T

A

A

K

R

V

V

F

F

E

D

S

G

E

E

E

R

E

A

A

A

L

L

E

D

A

A

I

L

L

E

G

D

G

G

K

T

V

I

K

T

E

V

G

G

D

D

V

A

V

V

V

V

I

I

R

R

Y

Y

E

E

G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

R

R

E
|
E

M

M

L

L

A

A

P

I

T

T

S

G

A

A

L

I

V

K

G

G

L

A

G

G

L

L

G

G

K

K

K

D

V

V

A

L

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S
|
S

G

G

G

G

T

T

R

T

G

G

L

L

S

C

I

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

A

V

E

D

G

G

G

G

A

P

I

I

A

A

L

L

V

L

E

R

D

N

G

G

D

D

K

R

I

I

K

R

I

L

D

D

I

V

E

A

N

G

R

R

K

V

L

L

D

D

L

V

E

L

V

A

S

D

E

P

E

A

E

E

L

F

A

A

E

S

R

R

R

Q

A

Q

K

D

A

F

K

S

K

P

K

P

E

P

A

P

R

R

E

Y

V

T

K

T

G

G

A

V

L

L

A

S

K

K

Y

Y

A

V

K

K

L

L

V

V

S

S

S

S

A

A

D

A

K

V

G

G

A

A

V

V

C64 (≠ G33) binding [2Fe-2S] cluster
D96 (= D65) binding Mg(2+)
C137 (= C106) binding [2Fe-2S] cluster
D138 (= D107) binding Mg(2+)
K139 (= K108) binding via carbamate group; modified: N6-carboxylysine
C214 (= C179) binding [2Fe-2S] cluster
E465 (= E432) binding Mg(2+)
S491 (= S458) mutation to A: Loss of catalytic activity.

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory