SitesBLAST
Comparing 14400 FitnessBrowser__Keio:14400 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
51% identity, 69% coverage: 6:246/348 of query aligns to 17:257/378 of P69874
- C26 (≠ R15) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F16) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ M34) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C43) mutation to T: Loss of ATPase activity and transport.
- L60 (= L49) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ I65) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V124) mutation to M: Loss of ATPase activity and transport.
- D172 (= D161) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
45% identity, 80% coverage: 7:283/348 of query aligns to 7:281/353 of 1vciA
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
47% identity, 73% coverage: 7:259/348 of query aligns to 7:268/375 of 2d62A
1g291 Malk (see paper)
43% identity, 88% coverage: 7:312/348 of query aligns to 4:332/372 of 1g291
- binding magnesium ion: D69 (= D66), E71 (= E68), K72 (≠ D69), K79 (≠ Q76), D80 (≠ Q77), E292 (= E278), D293 (≠ A279)
- binding pyrophosphate 2-: S38 (= S41), G39 (= G42), C40 (= C43), G41 (= G44), K42 (= K45), T43 (= T46), T44 (= T47)
Sites not aligning to the query:
8hplC Lpqy-sugabc in state 1 (see paper)
44% identity, 78% coverage: 9:280/348 of query aligns to 5:284/384 of 8hplC
8hprC Lpqy-sugabc in state 4 (see paper)
43% identity, 80% coverage: 4:280/348 of query aligns to 10:286/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F6), S38 (= S41), G39 (= G42), G41 (= G44), K42 (= K45), S43 (≠ T46), Q82 (= Q85), Q133 (= Q136), G136 (= G139), G137 (= G140), Q138 (= Q141), H192 (= H195)
- binding magnesium ion: S43 (≠ T46), Q82 (= Q85)
8hprD Lpqy-sugabc in state 4 (see paper)
43% identity, 80% coverage: 4:280/348 of query aligns to 10:286/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F6), S38 (= S41), C40 (= C43), G41 (= G44), K42 (= K45), S43 (≠ T46), T44 (= T47), Q82 (= Q85), R129 (= R132), Q133 (= Q136), S135 (= S138), G136 (= G139), G137 (= G140), Q159 (≠ E162), H192 (= H195)
- binding magnesium ion: S43 (≠ T46), Q82 (= Q85)
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 78% coverage: 9:280/348 of query aligns to 6:287/393 of P9WQI3
- H193 (= H195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 3:293/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 3:293/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F16), S37 (= S41), G38 (= G42), C39 (= C43), G40 (= G44), K41 (= K45), S42 (≠ T46), T43 (= T47), Q81 (= Q85), R128 (= R132), A132 (≠ Q136), S134 (= S138), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
- binding magnesium ion: S42 (≠ T46), Q81 (= Q85)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 3:293/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F16), G38 (= G42), C39 (= C43), G40 (= G44), K41 (= K45), S42 (≠ T46), T43 (= T47), R128 (= R132), S134 (= S138), Q137 (= Q141)
- binding beryllium trifluoride ion: S37 (= S41), G38 (= G42), K41 (= K45), Q81 (= Q85), S134 (= S138), G136 (= G140), H191 (= H195)
- binding magnesium ion: S42 (≠ T46), Q81 (= Q85)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 3:293/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F16), V17 (= V21), G38 (= G42), C39 (= C43), G40 (= G44), K41 (= K45), S42 (≠ T46), T43 (= T47), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
- binding tetrafluoroaluminate ion: S37 (= S41), G38 (= G42), K41 (= K45), Q81 (= Q85), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (= H195)
- binding magnesium ion: S42 (≠ T46), Q81 (= Q85)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 3:293/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F16), V17 (= V21), G38 (= G42), C39 (= C43), G40 (= G44), K41 (= K45), S42 (≠ T46), T43 (= T47), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
- binding magnesium ion: S42 (≠ T46), Q81 (= Q85)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 80% coverage: 7:284/348 of query aligns to 1:291/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F16), S35 (= S41), G36 (= G42), C37 (= C43), G38 (= G44), K39 (= K45), S40 (≠ T46), T41 (= T47), R126 (= R132), A130 (≠ Q136), S132 (= S138), G134 (= G140), Q135 (= Q141)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 80% coverage: 7:284/348 of query aligns to 4:294/371 of P68187
- A85 (= A88) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P109) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V117) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A120) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ L242) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (vs. gap) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G269) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (vs. gap) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (= G274) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 87% coverage: 7:309/348 of query aligns to 4:317/369 of P19566
- L86 (= L89) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ A298) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 86% coverage: 9:308/348 of query aligns to 6:315/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 86% coverage: 9:308/348 of query aligns to 6:315/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 86% coverage: 9:308/348 of query aligns to 6:315/353 of 1oxuA
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
36% identity, 86% coverage: 9:308/348 of query aligns to 6:315/353 of Q97UY8
- S142 (= S138) mutation to A: Decrease in ATPase activity. Can form dimers.
- G144 (= G140) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
- E166 (= E162) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).
Query Sequence
>14400 FitnessBrowser__Keio:14400
MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSE
GQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVPRAELKARVKEA
LAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIR
ELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDA
NLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSDRGEESQRCVIRHVAYM
GPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMFVLADAA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory