SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 14407 FitnessBrowser__Keio:14407 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
36% identity, 73% coverage: 95:571/655 of query aligns to 57:512/562 of 6ovtA

query
sites
6ovtA

Q

R

M

L

Q

A

A

N

A

A

A

V

K

K

E

E

-

G

I

V

T

F

R

S

N

A

G

G

G

G

I

Y

P

P

F

L

A

E

A

F

F

G

V

T

S

I

D

S

P

V

C

S

D
|
D

G
|
G

R

I

S

S

Q

M

G

G

T

H

H

E

G

G

M

M

F

H

D

F

S

S

L

L

P

V

Y

S

R

R

N

E

D

V

A

I

A

A

I

D

V

S

F

V

R

E

R

V

L

V

I

M

R

Q

S

A

L

E

P

R

T

L

R

D

R

G

A

S

V

V

I

L

G

-

V

L

A

A

T

G

C
|
C

D
|
D

K
|
K

G

S

L

L

P

P

A

G

T

M

M

L

I

M

A

A

L

-

A

A

A

A

M

R

H

L

D

D

L

L

P

A

T

A

I

V

L

F

V

L

P

Y

G

A

G

G

A

S

T

I

L

L

P

P

P

G

T

R

V

A

G

K

E

L

D

S

A

D

G

G

K

S

-

E

-

R

-

D

V

V

Q

T

T

I

I

I

G

D

A

A

R

F

F

E

A

A

N

V

H

G

E

A

L

C

S

S

-

R

-

G

L

L

Q

M

E

S

A

R

A

A

E

D

L

V

G

D

C

A

-

I

-

E

R

R

A

A

C

I

A

C

S

P

P

G

G

E

G

G

G
x
A

C
|
C

Q

G

F

G

L

M

G

Y

T

T

A

A

G

N

T

T

S

M

Q

A

V

S

V

A

A

A

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

H

G

S

S

A

A

L

A

A

P

P

P

S

A

G

T

Q

D

A

R

V

R

W

R

L

D

E

G

I

F

A

A

R

R

Q

R

S

S

A

G

R

Q

A

A

V

V

S

V

E

E

L

L

D

L

S

R

R

R

G

G

I

I

T

T

T

A

R

R

D

D

I

I

L

L

S

T

D

K

K

E

A

A

I

F

E

E

N

N

A

A

M

I

V

A

I

V

H

V

A

M

A

A

F

F

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

L

H

H

I

L

P

L

A

A

I

I

A

A

H

H

A

E

A

A

G

N

C

V

T

A

I

L

P

-

D

S

V

L

E

Q

H

D

W

F

T

S

R

R

I

I

N

G

R

S

K

G

V

V

P

P

R

H

L

L

V

A

S

D

V

V

L

K

P

P

N

F

G

G

P

R

D

H

Y

V

H

M

P

S

T

D

V

V

R

D

A

H

F

I

L

-

A

-

G

G

G

G

V

V

P

P

E

V

V

V

M

M

L

K

H

A

L

L

R

L

D

D

L

A

G

G

L

L

L

L

H

H

L

G

D

D

A

C

M

L

T

T

V

V

T

T

G

G

Q

H

T

T

V

M

G

A

E

E

N

N

L

L

E

A

W

A

W

I

Q

T

A

P

S

P

E

D

R

P

R

D

A

G

R

K

F

V

R

L

Q

R

C

A

L

L

R

-

E

-

Q

A

D

N

G

P

V

I

E

H

P

P

D

-

D

-

V

-

I

-

L

-

P

-

P

-

E

-

K

-

A

-

K

-

A

-

K

-

G

-

L

-

T

S

S

G

T

G

V

I

C

T

F

I

P

L

T

H

G

G

N

S

I

L

A

A

P

P

E

E

G

G

S

A

V

V

I

V

K

K

A

T

T

A

A

G

I

F

D

D

P

S

S

D

V

V

V

-

G

-

E

-

D

-

G

-

V

-

Y

-

H

-

H

F

T

E

G

G

R

T

V

A

R

R

V

V

F

F

V

D

S

G

E

E

A

R

Q

A

A

A

I

L

K

D

A

A

I

L

K

E

R

D

E

G

E

T

I

I

V

T

Q

V

G

G

D

D

I

A

M

V

V

V

V

I

I

R

G

Y

G

E

G

G

P

P

S

K

-

G

G

G

T

P

G

G

M

M

E

R

E
|
E

T

M

Y

L

Q

A

L

I

T

T

S

G

A

A

L

I

K

K

H

G

I

A

S

G

W

L

G

G

K

K

T

D

V

V

S

L

L

L

I

L

T

T

D

D

A

G

R

R

F

F

S

S

G

G

V

G

S

T

T

T

G

G

A

L

C

C

F

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

L

V

A

D

G

G

G

G

P

P

I

I

G

A

K

L

L

L

R

R

D

N

N

G

D

D

I

R

I

I

E

R

I

L

A

D

V

V

binding fe2/s2 (inorganic) cluster: D83 (= D120), G84 (= G121), C124 (= C162), D125 (= D163), A200 (≠ G232), C201 (= C233)
binding magnesium ion: D83 (= D120), D125 (= D163), K126 (= K164), E452 (= E511)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 51

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 73% coverage: 95:571/655 of query aligns to 70:525/575 of P9WKJ5

query
sites
P9WKJ5

Q

R

M

L

Q

A

A

N

A

A

A

V

K

K

E

E

-

G

I

V

T

F

R

S

N

A

G

G

G

G

I

Y

P

P

F

L

A

E

A

F

F

G

V

T

S

I

D

S

P

V

C

S

D
|
D

G

G

R

I

S

S

Q

M

G

G

T

H

H

E

G

G

M

M

F

H

D

F

S

S

L

L

P

V

Y

S

R

R

N

E

D

V

A

I

A

A

I

D

V

S

F

V

R

E

R

V

L

V

I

M

R

Q

S

A

L

E

P

R

T

L

R

D

R

G

A

S

V

V

I

L

G

-

V

L

A

A

T

G

C
|
C

D
|
D

K
|
K

G

S

L

L

P

P

A

G

T

M

M

L

I

M

A

A

L

-

A

A

A

A

M

R

H

L

D

D

L

L

P

A

T

A

I

V

L

F

V

L

P

Y

G

A

G

G

A

S

T

I

L

L

P

P

P

G

T

R

V

A

G

K

E

L

D

S

A

D

G

G

K

S

-

E

-

R

-

D

V

V

Q

T

T

I

I

I

G

D

A

A

R

F

F

E

A

A

N

V

H

G

E

A

L

C

S

S

-

R

-

G

L

L

Q

M

E

S

A

R

A

A

E

D

L

V

G

D

C

A

-

I

-

E

R

R

A

A

C

I

A

C

S

P

P

G

G

E

G

G

G

A

C
|
C

Q

G

F

G

L

M

G

Y

T

T

A

A

G

N

T

T

S

M

Q

A

V

S

V

A

A

A

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

H

G

S

S

A

A

L

A

A

P

P

P

S

A

G

T

Q

D

A

R

V

R

W

R

L

D

E

G

I

F

A

A

R

R

Q

R

S

S

A

G

R

Q

A

A

V

V

S

V

E

E

L

L

D

L

S

R

R

R

G

G

I

I

T

T

T

A

R

R

D

D

I

I

L

L

S

T

D

K

K

E

A

A

I

F

E

E

N

N

A

A

M

I

V

A

I

V

H

V

A

M

A

A

F

F

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

L

H

H

I

L

P

L

A

A

I

I

A

A

H

H

A

E

A

A

G

N

C

V

T

A

I

L

P

-

D

S

V

L

E

Q

H

D

W

F

T

S

R

R

I

I

N

G

R

S

K

G

V

V

P

P

R

H

L

L

V

A

S

D

V

V

L

K

P

P

N

F

G

G

P

R

D

H

Y

V

H

M

P

S

T

D

V

V

R

D

A

H

F

I

L

-

A

-

G

G

G

G

V

V

P

P

E

V

V

V

M

M

L

K

H

A

L

L

R

L

D

D

L

A

G

G

L

L

L

L

H

H

L

G

D

D

A

C

M

L

T

T

V

V

T

T

G

G

Q

H

T

T

V

M

G

A

E

E

N

N

L

L

E

A

W

A

W

I

Q

T

A

P

S

P

E

D

R

P

R

D

A

G

R

K

F

V

R

L

Q

R

C

A

L

L

R

-

E

-

Q

A

D

N

G

P

V

I

E

H

P

P

D

-

D

-

V

-

I

-

L

-

P

-

P

-

E

-

K

-

A

-

K

-

A

-

K

-

G

-

L

-

T

S

S

G

T

G

V

I

C

T

F

I

P

L

T

H

G

G

N

S

I

L

A

A

P

P

E

E

G

G

S

A

V

V

I

V

K

K

A

T

T

A

A

G

I

F

D

D

P

S

S

D

V

V

V

-

G

-

E

-

D

-

G

-

V

-

Y

-

H

-

H

F

T

E

G

G

R

T

V

A

R

R

V

V

F

F

V

D

S

G

E

E

A

R

Q

A

A

A

I

L

K

D

A

A

I

L

K

E

R

D

E

G

E

T

I

I

V

T

Q

V

G

G

D

D

I

A

M

V

V

V

V

I

I

R

G

Y

G

E

G

G

P

P

S

K

-

G

G

G

T

P

G

G

M

M

E

R

E
|
E

T

M

Y

L

Q

A

L

I

T

T

S

G

A

A

L

I

K

K

H

G

I

A

S

G

W

L

G

G

K

K

T

D

V

V

S

L

L

L

I

L

T

T

D

D

A

G

R

R

F

F

S
|
S

G

G

V

G

S

T

T

T

G

G

A

L

C

C

F

V

G

G

H

H

V

I

S

A

P

P

E

E

A

A

L

V

A

D

G

G

G

G

P

P

I

I

G

A

K

L

L

L

R

R

D

N

N

G

D

D

I

R

I

I

E

R

I

L

A

D

V

V

D96 (= D120) binding
C137 (= C162) binding
D138 (= D163) binding
K139 (= K164) binding via carbamate group; modified: N6-carboxylysine
C214 (= C233) binding
E465 (= E511) binding
S491 (= S537) mutation to A: Loss of catalytic activity.

Sites not aligning to the query:

64 binding

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 83% coverage: 94:638/655 of query aligns to 105:607/608 of Q9LIR4

query
sites
Q9LIR4

M

L

Q

K

M

L

Q

S

A

E

A

A

A

V

K

K

E

E

I

G

T

V

R

E

N

N

G

A

G

G

-

M

I

V

P

G

F

F

A

R

A

F

F

N

V

T

S

I

D

G

P

V

C

S

D

D

G

A

R

I

S

S

Q

M

G

G

T

T

H

R

G

G

M

M

F

C

D

F

S

S

L

L

P

Q

Y

S

R

R

N

D

D

L

A

I

A

A

I

D

V

S

F

I

R

E

R

T

L

V

I

M

R

-

S

S

L

A

P

Q

T

W

R

Y

R

D

A

G

V

N

I

I

G

S

V

I

A

P

T

G

C
|
C

D
|
D

K

K

G

N

L

M

P

P

A

G

T

T

M

I

I

M

A

A

L

M

A

G

A

R

M

L

H

N

D

R

L

-

P

P

T

G

I

I

L

M

V

V

P

Y

G

G

G

G

A

T

T

I

L

K

P

P

P

G

T

H

V

F

G

Q

E

D

D

K

A

T

G

Y

K

D

V

I

-

V

-

S

-

A

-

F

Q

Q

T

S

I

Y

G

G

A

-

R

E

F

F

A

V

N

S

H

G

E

S

L

I

S

S

L

D

Q

E

E

Q

A

R

A

K

E

T

L

V

G

L

C

H

R

H

A

S

C

C

A

-

S

-

P

P

G

G

G

A

G

G

-

A

C
|
C

Q

G

F

G

L

M

G

Y

T

T

A

A

G

N

T

T

S

M

Q

A

V

S

V

A

A

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

H

Y

S

S

A

S

L

S

A

I

P

P

S

A

G

E

Q

D

A

P

V

L

W

K

L

L

E

D

I

E

A

C

R

R

Q

L

S

A

A

G

R

K

A

Y

V

L

S

L

E

E

L

L

D

L

S

K

R

M

G

D

I

L

T

K

T

P

R

R

D

D

I

I

L

I

S

T

D

P

K

K

A

S

I

L

E

R

N

N

A

A

M

M

V

V

I

S

H

V

A

M

A

A

F

L

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

L

H

H

I

L

P

I

A

A

I

I

A

A

H

R

A

S

A

V

G

G

C

L

T

E

I

L

P

-

D

T

V

L

E

D

H

D

W

F

T

Q

R

K

I

V

N

S

R

D

K

A

V

V

P

P

R

F

L

L

V

A

S

D

V

L

L

K

P

P

N

S

G

G

P

K

D

Y

Y

V

H

M

P

E

T

D

V

I

R

H

A

K

F

I

L

-

A

-

G

G

G

G

V

T

P

P

E

A

V

V

M

L

L

R

H

Y

L

L

R

L

D

E

L

L

G

G

L

L

L

M

H

D

L

G

D

D

A

C

M

M

T

T

V

V

T

T

G

G

Q

Q

T

T

V

L

G

A

E

Q

N

N

L

L

E

E

W

N

W

V

Q

P

A

S

S

L

E

-

R

-

R

-

A

-

R

-

F

-

R

-

Q

-

C

-

L

-

R

-

E

-

Q

-

D

-

G

-

V

T

E

E

P

G

D

Q

D

E

V

I

I

I

L

R

P

P

P

L

E

S

K

N

A

P

K

I

A

K

K

E

G

-

L

-

T

T

S

G

T

H

V

I

C

Q

F

I

P

L

T

R

G

G

N

D

I

L

A

A

P

P

E

D

G

G

S

S

V

V

I

A

K

K

A

I

T

T

A

-

I

-

D

-

P

-

S

-

V

-

V

-

G

G

E

K

D

E

G

G

V

L

Y

Y

H

-

H

F

T

S

G

G

R

P

V

A

R

L

V

V

F

F

V

E

S

G

E

E

A

E

Q

S

A

M

I

L

K

A

A

A

I

I

K

S

R

A

E

D

E

P

I

M

-

S

V

F

Q

K

G

G

D

T

I

V

M

V

V

V

V

I

I

R

G

G

G

E

G

G

P

P

S

K

-

G

G

G

T

P

G

G

M

M

E

P

E
|
E

T

M

Y

L

Q

T

L

P

T

T

S

S

A

A

L

I

K

M

H

G

I

A

S

G

W

L

G

G

K

K

T

E

V

C

S

A

L

L

I

L

T

T

D

D

A

G

R

R

F

F

S

S

G

G

V

G

S

S

T

H

G

G

A

F

C

V

F

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

L

Q

A

E

G

G

G

G

P

P

I

I

G

G

K

L

L

I

R

K

D

N

N

G

D

D

I

I

I

I

E

T

I

I

A

D

V

I

D

G

R

K

L

K

T

R

L

I

T

-

G

-

S

-

V

-

N

-

F

-

I

-

G

-

T

-

A

-

D

D

N

T

P

Q

L

V

T

S

P

P

E

E

E

E

G

-

A

M

R

N

E

D

L

R

A

R

R

K

R

K

Q

W

T

T

H

A

P

P

D

A

L

Y

H

K

A

V

H

N

D

R

F

G

L

V

P

-

D

-

D

-

T

-

R

-

L

L

W

Y

A

K

A

Y

L

I

Q

K

S

N

V

V

S

Q

G

S

G

A

T

S

W

-

K

D

G

G

C

C

I

V

Y

T

D

D

C173 (= C162) binding
D174 (= D163) binding
C245 (= C233) binding
E497 (= E511) binding

Sites not aligning to the query:

100 binding

5ze4A The structure of holo- structure of dhad complex with [2fe-2s] cluster (see paper)
30% identity, 83% coverage: 94:638/655 of query aligns to 66:568/569 of 5ze4A

query
sites
5ze4A

M

L

Q

K

M

L

Q

S

A

E

A

A

A

V

K

K

E

E

I

G

T

V

R

E

N

N

G

A

G

G

-

M

I

V

P

G

F

F

A

R

A

F

F

N

V

T

S

I

D

G

P

V

C
x
S

D
|
D

G
x
A

R

I

S

S

Q

M

G

G

T

T

H

R

G

G

M

M

F

C

D

F

S

S

L

L

P

Q

Y

S

R

R

N

D

D

L

A

I

A

A

I

D

V

S

F

I

R

E

R

T

L

V

I

M

R

-

S

S

L

A

P

Q

T

W

R

Y

R

D

A

G

V

N

I

I

G

S

V

I

A

P

T

G

C
|
C

D
|
D

K

K

G

N

L

M

P

P

A

G

T

T

M

I

I

M

A

A

L

M

A

G

A

R

M

L

H

N

D

R

L

-

P

P

T

G

I

I

L

M

V

V

P

Y

G

G

G

G

A

-

T

T

L

I

P

K

P

P

T

G

V

H

G

F

E

Q

D

D

-

K

-

T

-

Y

-

D

-

I

A

V

G

S

K

A

V

F

Q

Q

T

S

I

Y

G

G

A

-

R

E

F

F

A

V

N

S

H

G

E

S

L

I

S

S

L

D

Q

E

E

Q

A

R

A

K

E

T

L

V

G

L

C

H

R

H

A

S

C

C

A

-

S

-

P

P

G

G

G

A

G

G

-
x
A

C
|
C

Q

G

F

G

L

M

G

Y

T

T

A

A

G

N

T

T

S

M

Q

A

V

S

V

A

A

I

E

E

A

A

L

M

G

G

L

M

A

S

L

L

P

P

H

Y

S

S

A

S

L

S

A

I

P

P

S

A

G

E

Q

D

A

P

V

L

W

K

L

L

E

D

I

E

A

C

R

R

Q

L

S

A

A

G

R

K

A

Y

V

L

S

L

E

E

L

L

D

L

S

K

R

M

G

D

I

L

T

K

T

P

R

R

D

D

I

I

L

I

S

T

D

P

K

K

A

S

I

L

E

R

N

N

A

A

M

M

V

V

I

S

H

V

A

M

A

A

F

L

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

L

H

H

I

L

P

I

A

A

I

I

A

A

H

R

A

S

A

V

G

G

C

L

T

E

I

L

P

-

D

T

V

L

E

D

H

D

W

F

T

Q

R

K

I

V

N

S

R

D

K

A

V

V

P

P

R

F

L

L

V

A

S

D

V

L

L

K

P

P

N

S

G

G

P

K

D

Y

Y

V

H

M

P

E

T

D

V

I

R

H

A

K

F

I

L

-

A

-

G

G

G

G

V

T

P

P

E

A

V

V

M

L

L

R

H

Y

L

L

R

L

D

E

L

L

G

G

L

L

L

M

H

D

L

G

D

D

A

C

M

M

T

T

V

V

T

T

G

G

Q

Q

T

T

V

L

G

A

E

Q

N

N

L

L

E

E

W

N

W

V

Q

P

A

S

S

L

E

-

R

-

R

-

A

-

R

-

F

-

R

-

Q

-

C

-

L

-

R

-

E

-

Q

-

D

-

G

-

V

T

E

E

P

G

D

Q

D

E

V

I

I

I

L

R

P

P

P

L

E

S

K

N

A

P

K

I

A

K

K

E

G

-

L

-

T

T

S

G

T

H

V

I

C

Q

F

I

P

L

T

R

G

G

N

D

I

L

A

A

P

P

E

D

G

G

S

S

V

V

I

A

K

K

A

I

T

T

A

-

I

-

D

-

P

-

S

-

V

-

V

-

G

G

E

K

D

E

G

G

V

L

Y

Y

H

-

H

F

T

S

G

G

R

P

V

A

R

L

V

V

F

F

V

E

S

G

E

E

A

E

Q

S

A

M

I

L

K

A

A

A

I

I

K

S

R

A

E

D

E

P

I

M

-

S

V

F

Q

K

G

G

D

T

I

V

M

V

V

V

V

I

I

R

G

G

G

E

G

G

P

P

S

K

-

G

G

G

T

P

G

G

M

M

E

P

E
|
E

T

M

Y

L

Q

T

L

P

T

T

S

S

A

A

L

I

K

M

H

G

I

A

S

G

W

L

G

G

K

K

T

E

V

C

S

A

L

L

I

L

T

T

D

D

A

G

R

R

F

F

S

S

G

G

V

G

S

S

T

H

G

G

A

F

C

V

F

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

L

Q

A

E

G

G

G

G

P

P

I

I

G

G

K

L

L

I

R

K

D

N

N

G

D

D

I

I

I

I

E

T

I

I

A

D

V

I

D

G

R

A

L

A

T

R

L

I

T

-

G

-

S

-

V

-

N

-

F

-

I

-

G

-

T

-

A

-

D

D

N

T

P

Q

L

V

T

S

P

P

E

E

E

E

G

-

A

M

R

N

E

D

L

R

A

R

R

K

R

K

Q

W

T

T

H

A

P

P

D

A

L

Y

H

K

A

V

H

N

D

R

F

G

L

V

P

-

D

-

D

-

T

-

R

-

L

L

W

Y

A

K

A

Y

L

I

Q

K

S

N

V

V

S

Q

G

S

G

A

T

S

W

-

K

D

G

G

C

C

I

V

Y

T

D

D

binding fe2/s2 (inorganic) cluster: S92 (≠ C119), D93 (= D120), A94 (≠ G121), C134 (= C162), D135 (= D163), A205 (vs. gap), C206 (= C233)
binding magnesium ion: D93 (= D120), D135 (= D163), E458 (= E511)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 59, 61

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
32% identity, 81% coverage: 77:608/655 of query aligns to 42:541/579 of B5ZZ34

query
sites
B5ZZ34

D

D

G

G

R

R

P

P

I

V

A

I

L

G

G

I

Y

L

H

N

T

T

-

W

-

S

-

D

-

M

-

T

-

P

-
x
C

-

N

G

G

H

H

W

L

-

R

E

E

I

L

G

A

M

E

Q

K

M

V

Q

K

A

A

A

G

A

V

K

W

E

E

I

A

T

-

R

-

N

-

G

G

G

G

I

F

P

P

F

L

A

E

A

V

F

P

V

V

S

F

D

S

P

A

C

S

D
x
E

G

N

R

T

S

F

Q

R

G

P

T

T

H

A

G

M

M

M

F

-

D

-

S

-

L

-

P

-

Y

Y

R

R

N

N

D

L

A

A

A

A

I

L

V

A

F

V

R

E

R

E

L

A

I

I

R

R

S

G

L

Q

P

P

T

M

R

D

R

G

A

C

V

V

I

L

G

L

V

V

A

G

T

-

C
|
C

D
|
D

K

K

G

T

L

T

P

P

A

S

T

L

M

L

I

M

A

G

L

-

A

A

A

A

M

S

H

C

D

D

L

L

P

P

T

S

I

I

L

V

V

V

P

T

G

G

G

G

A

P

T

M

L

L

P

N

P

G

T

Y

V

F

-

R

G

G

E

E

D

R

A

V

G

G

K

S

-

G

-

T

-

H

V

L

Q

W

T

K

I

F

G

S

A

E

R

M

F

V

A

K

N

A

H

G

E

E

L

M

S

T

L

Q

Q

A

E

E

A

F

A

L

E

E

L

A

G

E

C

A

R

S

A

M

C

S

A

R

S

S

P

-

G

S

G

G

G

T

C
|
C

Q

N

F

T

L

M

G

G

T

T

A

A

G

S

T

T

S

M

Q

A

V

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

H

G

S

N

A

A

L

A

A

I

P

P

S

G

G

V

Q

D

A

S

V

R

W

R

L

K

E

V

I

M

A

A

R

Q

Q

L

S

T

A

G

R

R

A

R

V

I

S

V

E

Q

L

M

D

V

S

K

R

D

G

D

I

L

T

K

T

P

R

S

D

E

I

I

L

M

S

T

D

K

K

Q

A

A

I

F

E

E

N

N

A

A

M

I

V

R

I

T

H

N

A

A

A

A

F

I

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

A

R

A

V

G

G

C

I

T

D

I

L

P

-

D

S

V

L

E

D

H

D

W

W

T

D

R

R

I

C

N

G

R

R

K

D

V

V

P

P

R

T

L

I

V

V

S

N

V

L

L

M

P

P

N

S

G

G

P

K

D

-

Y

-

H

Y

P

L

T

M

V

E

R

E

A

F

F

F

L

Y

A

A

G

G

G

G

V

L

P

P

E

V

V

V

M

L

L

K

H

R

L

L

R

G

D

E

L

A

G

G

L

L

L

L

H

H

L

K

D

D

A

A

M

L

T

T

V

V

T

S

G

G

Q

E

T

T

V

V

G

-

E

-

N

-

L

-

E

-

W

-

W

W

Q

D

A

E

S

V

E

K

R

D

R

V

A

V

R

N

F

W

R

N

Q

E

C

-

L

-

R

-

E

-

Q

-

D

-

G

-

V

-

E

-

P

-

D

-

D

D

V

V

I

I

L

L

P

P

P

A

E

E

K

K

A

A

K

-

A

-

K

-

G

-

L

L

T

T

S

S

T

S

-

G

-

G

V

I

C

V

F

V

P

L

T

R

G

G

N

N

I

L

A

A

P

P

E

K

G

G

S

A

V

V

I

L

K

K

A

P

T

S

A

A

I

A

D

S

P

P

S

H

V

L

V

L

G

-

E

-

D

-

G

-

V

-

Y

-

H

V

H

H

T

K

G

G

R

R

V

A

R

V

V

V

F

F

-

E

-

D

V

I

S

D

E

D

A

Y

Q

K

A

A

I

K

K

I

A

N

I

D

K

D

R

N

E

L

E

D

I

I

V

D

Q

E

G

N

D
x
C

I

I

M

M

V

V

V

M

I

K

G

N

G
x
C

G

G

P

P

S

K

G

G

T

Y

-

P

G

G

M

M

E

A

E
|
E

T

V

Y

G

Q

N

L

M

T

G

S

L

A

P

L

P

K

K

H

V

I

L

S

K

W

K

G

G

-

I

K

L

T

D

V

M

S

V

L

R

I

I

T

S

D

D

A

A

R

R

F

M

S
|
S

G

G

V

T

S

A

T

Y

G

G

A

T

C

V

F

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

L

A

A

V

G

G

G

G

P

P

I

L

G

A

K

V

L

V

R

K

D

N

N

G

D

D

I

M

I

I

E

E

I

L

A

D

V

V

-

P

-

N

D

R

R

R

L

L

T

H

L

L

T

D

G

-

S

-

V

-

N

-

F

-

I

-

G

-

T

-

A

-

D

-

N

-

P

-

L

I

T

S

P

D

E

E

E

E

G

L

A

A

R

R

E

R

L

L

A

A

R

E

R

W

Q

Q

T

P

H

N

P

H

D

D

L

L

C59 (vs. gap) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ D120) binding
C127 (= C162) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D163) binding
C200 (= C233) binding ; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (≠ D495) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ G502) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E511) binding
S480 (= S537) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
32% identity, 81% coverage: 77:608/655 of query aligns to 39:538/576 of 5j85A

query
sites
5j85A

D

D

G

G

R

R

P

P

I

V

A

I

L

G

G

I

Y

L

H

N

T

T

-

W

-

S

-

D

-

M

-

T

-

P

-
x
C

-

N

G

G

H

H

W

L

-

R

E

E

I

L

G

A

M

E

Q

K

M

V

Q

K

A

A

A

G

A

V

K

W

E

E

I

A

T

-

R

-

N

-

G

G

G

G

I

F

P

P

F

L

A

E

A

V

F

P

V

V

S

F

D

S

P

A

C

S

D
x
E

G
x
N

R

T

S

F

Q

R

G

P

T

T

H

A

G

M

M

M

F

-

D

-

S

-

L

-

P

-

Y

Y

R

R

N

N

D

L

A

A

A

A

I

L

V

A

F

V

R

E

R

E

L

A

I

I

R

R

S

G

L

Q

P

P

T

M

R

D

R

G

A

C

V

V

I

L

G

L

V

V

A

G

T

-

C
|
C

D
|
D

K
|
K

G

T

L

T

P

P

A

S

T

L

M

L

I

M

A

G

L

-

A

A

A

A

M

S

H

C

D

D

L

L

P

P

T

S

I

I

L

V

V

V

P

T

G

G

G

G

A

P

T

M

L

L

P

N

P

G

T

Y

V

F

-

R

G

G

E

E

D

R

A

V

G

G

K

S

-

G

-

T

-

H

V

L

Q

W

T

K

I

F

G

S

A

E

R

M

F

V

A

K

N

A

H

G

E

E

L

M

S

T

L

Q

Q

A

E

E

A

F

A

L

E

E

L

A

G

E

C

A

R

S

A

M

C

S

A

R

S

S

P

-

G

S

G

G

G
x
T

C
|
C

Q

N

F

T

L

M

G

G

T
|
T

A

A

G

S

T

T

S

M

Q

A

V

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

A

A

L

L

P

S

H

G

S

N

A

A

L

A

A

I

P

P

S

G

G

V

Q

D

A

S

V

R

W

R

L

K

E

V

I

M

A

A

R

Q

Q

L

S

T

A

G

R

R

A

R

V

I

S

V

E

Q

L

M

D

V

S

K

R

D

G

D

I

L

T

K

T

P

R

S

D

E

I

I

L

M

S

T

D

K

K

Q

A

A

I

F

E

E

N

N

A

A

M

I

V

R

I

T

H

N

A

A

A

A

F

I

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

A

R

A

V

G

G

C

I

T

D

I

L

P

-

D

S

V

L

E

D

H

D

W

W

T

D

R

R

I

C

N

G

R

R

K

D

V

V

P

P

R

T

L

I

V

V

S

N

V

L

L

M

P

P

N

S

G

G

P

K

D

-

Y

-

H

Y

P

L

T

M

V

E

R

E

A

F

F

F

L

Y

A

A

G

G

G

G

V

L

P

P

E

V

V

V

M

L

L

K

H

R

L

L

R

G

D

E

L

A

G

G

L

L

L

L

H

H

L

K

D

D

A

A

M

L

T

T

V

V

T

S

G

G

Q

E

T

T

V

V

G

-

E

-

N

-

L

-

E

-

W

-

W

W

Q

D

A

E

S

V

E

K

R

D

R

V

A

V

R

N

F

W

R

N

Q

E

C

-

L

-

R

-

E

-

Q

-

D

-

G

-

V

-

E

-

P

-

D

-

D

D

V

V

I

I

L

L

P

P

P

A

E

E

K

K

A

A

K

-

A

-

K

-

G

-

L

L

T

T

S

S

T

S

-

G

-

G

V

I

C

V

F

V

P

L

T

R

G

G

N

N

I

L

A

A

P

P

E

K

G

G

S

A

V

V

I

L

K

K

A

P

T

S

A

A

I

A

D

S

P

P

S

H

V

L

V

L

G

-

E

-

D

-

G

-

V

-

Y

-

H

V

H

H

T

K

G

G

R

R

V

A

R

V

V

V

F

F

-

E

-

D

V

I

S

D

E

D

A

Y

Q

K

A

A

I

K

K

I

A

N

I

D

K

D

R

N

E

L

E

D

I

I

V

D

Q

E

G

N

D

C

I

I

M

M

V

V

V

M

I

K

G

N

G

C

G

G

P

P

S

K

G

G

T

Y

-

P

G

G

M

M

E

A

E
|
E

T

V

Y

G

Q

N

L

M

T

G

S

L

A

P

L

P

K

K

H

V

I

L

S

K

W

K

G

G

-

I

K

L

T

D

V

M

S

V

L

R

I

I

T

S

D

D

A

A

R

R

F

M

S

A

G

G

V

T

S

A

T

Y

G

G

A

T

C

V

F

V

G

L

H

H

V

T

S

S

P

P

E

E

A

A

L

A

A

V

G

G

G

G

P

P

I

L

G

A

K

V

L

V

R

K

D

N

N

G

D

D

I

M

I

I

E

E

I

L

A

D

V

V

-

P

-

N

D

R

R

R

L

L

T

H

L

L

T

D

G

-

S

-

V

-

N

-

F

-

I

-

G

-

T

-

A

-

D

-

N

-

P

-

L

I

T

S

P

D

E

E

E

E

G

L

A

A

R

R

E

R

L

L

A

A

R

E

R

W

Q

Q

T

P

H

N

P

H

D

D

L

L

binding fe2/s2 (inorganic) cluster: C56 (vs. gap), N89 (≠ G121), C124 (= C162), D125 (= D163), T196 (≠ G232), C197 (= C233)
binding magnesium ion: E88 (≠ D120), D125 (= D163), K126 (= K164), T202 (= T238), E450 (= E511)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
32% identity, 72% coverage: 103:576/655 of query aligns to 71:517/583 of Q1JUQ1

query
sites
Q1JUQ1

I

I

T

S

R

E

N

A

G

G

G

G

I

F

P

P

-

V

-

E

F

F

A

P

A

V

F

F

V

S

S

N

D

G

P

E

C

S

D

N

G

L

R

R

S

P

Q

S

G

-

T

-

H

-

G

-

M

-

F

-

D

-

S

A

L

M

P

L

Y

T

R

R

N

N

D

L

A

A

A

S

I

M

V

D

F

V

R

E

R

E

L

A

I

I

R

R

S

G

L

N

P

P

T

I

R

D

R

-

A

A

V

V

I

V

G

L

V

L

A

A

T

G

C
|
C

D

D

K

K

G

T

L

T

P

P

A

A

T

L

M

L

I

M

A

G

L

-

A

A

A

A

M

S

H

C

D

D

L

V

P

P

T

A

I

I

L

V

V

V

P

S

G

G

G

G

A

P

T

M

L

L

P

N

P

G

T

K

V

L

-

E

G

G

E

K

D

N

A

I

G

G

K

S

-

G

-

T

-

A

V

V

Q

W

T

Q

I

L

G

H

A

E

R

A

F

L

A

K

N

A

H

G

E

E

L

I

S

D

L

V

Q

H

E

H

-

F

-

L

A

S

A

A

E

E

L

A

G

G

C

M

R

S

A

R

C

S

A

A

S

-

P

-

G

-

G

G

G

T

C
|
C

Q

N

F

T

L

M

G

G

T

T

A

A

G

S

T

T

S

M

Q

A

V

C

V

M

A

A

E

E

A

A

L

L

G

G

L

V

A

A

L

L

P

P

H

H

S

N

A

A

L

A

A

I

P

P

S

A

G

V

Q

D

A

S

V

R

W

R

L

Y

E

V

I

L

A

A

R

H

Q

M

S

S

A

G

R

I

A

R

V

I

S

V

E

E

L

M

D

A

S

L

R

E

G

G

I

L

T

V

T

L

R

S

D

K

I

I

L

L

S

T

D

R

K

A

A

A

I

F

E

E

N

N

A

A

M

I

V

R

I

A

H

N

A

A

A

A

F

I

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

I

H

H

I

L

P

K

A

A

I

I

A

A

H

G

A

R

A

I

G

G

C

V

T

P

I

L

P

-

D

E

V

L

E

E

H

D

W

W

T

M

R

R

I

I

N

G

R

R

K

D

V

T

P

P

R

T

L

I

V

V

S

D

V

L

L

M

P

P

N

S

G

G

P

-

D

-

Y

R

H

F

P

P

T

M

V

E

R

E

A

F

F

Y

L

Y

A

A

G

G

G

G

V

L

P

P

E

A

V

V

M

L

L

R

H

R

L

L

R

G

D

E

L

G

G

G

L

L

L

L

-

P

H

N

L

P

D

D

A

A

M

L

T

T

V

V

T

N

G

G

Q

K

T

S

V

L

G

W

E

D

N

N

L

-

E

-

W

-

W

-

Q

-

A

-

S

-

E

-

R

-

R

-

A

-

R

-

F

-

R

-

Q

-

C

-

L

V

R

R

E

E

Q

A

D

P

G

N

V

Y

E

D

P

-

D

E

D

E

V

V

I

I

L

R

P

P

P

L

E

D

K

R

A

P

K

L

A

I

K

A

G

-

L

-

T

D

S

G

T

G

V

I

C

R

F

I

P

L

T

R

G

G

N

N

I

L

A

A

P

P

E

R

G

G

S

A

V

V

I

L

K

K

A

P

T

S

A

A

I

A

D

S

P

P

S

E

V

L

V

L

G

-

E

-

D

-

G

-

V

-

Y

-

H

K

H

H

T

R

G

G

R

R

V

A

R

V

V

V

F

F

V

E

S

N

E

L

A

D

Q

H

A

Y

I

K

K

A

A

T

I

I

K

N

R

D

E

E

-

A

-

L

E

D

I

I

V

D

Q

A

G

S

D

S

I

V

M

M

V

V

V

L

I

K

G

N

G
x
C

G

G

P

P

S

R

G

G

T

Y

-

P

G

G

M

M

E

A

E

E

T

V

Y

G

Q

N

L

M

T

G

S

L

A

P

L

P

K

K

H

L

I

L

S

R

W

Q

G

G

-

V

K

K

T

D

V

M

S

V

L

R

I

I

T

S

D

D

A

A

R

R

F

M

S

S

G

G

V

T

S

A

T

Y

G

G

A

T

C

V

F

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

L

A

A

A

G

G

G

G

P

P

I

L

G

A

K

A

L

V

R

R

D

N

N

G

D

D

I

W

I

I

E

E

I

L

A

D

V

C

D

E

R

A

L

G

T

T

L

L

C124 (= C162) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C233) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ G502) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

Sites not aligning to the query:

47 C→A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
56 C→A: Loss of activity but seems to be able to bind a Fe-S cluster.

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
32% identity, 67% coverage: 132:571/655 of query aligns to 90:504/569 of 8epzA

query
sites
8epzA

S

A

L

M

P

L

Y

F

R

R

N

N

D

L

A

A

A

S

I

M

V

D

F

V

R

E

R

E

L

S

I

I

R

R

S

G

L

N

P

P

T

L

R

D

R

-

A

G

V

V

I

V

G

L

V

L

A

M

T

G

C
|
C

D
|
D

K
|
K

G

T

L

T

P

P

A

S

T

L

M

M

I

M

A

G

L

-

A

A

A

A

M

S

H

C

D

D

L

L

P

P

T

T

I

I

L

G

V

V

P

S

G

G

G

G

A

P

T

M

L

L

P

S

P

G

T

K

V

F

-

R

G

G

E

R

D

E

A

L

G

G

K

S

-

G

-

T

-

D

V

V

Q
x
W

T

K

I

M

G

S

A

E

R

E

F

V

A

R

N

A

H

G

E

Q

L

M

S

S

L

Q

Q

E

E

E

A

F

A

F

E

E

L

-

G

-

C

A

R

E

A

S

C

C

A

M

S

H

P

R

G

S

G

H

G

G

-
x
H

C
|
C

Q

M

F

T

L

M

G

G

T

T

A

A

G

S

T

T

S

M

Q

A

V

S

V

M

A

V

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

H

G

S

N

A

A

L

A

A

I

P

P

S

A

G

V

Q

D

A

A

V

R

W

R

L

N

E

L

I

L

A

A

R

R

Q

A

S

S

A

G

R

R

A

R

V

I

S

V

E

Q

L

M

D

V

S

K

R

D

G

D

I

L

T

V

T

M

R

S

D

K

I

I

L

L

S

T

D

R

K

Q

A

A

I

F

E

E

N

N

A

A

M

I

V

R

I

V

H

N

A

A

A

A

F

I

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

A

R

A

I

G

G

C

V

T

D

I

L

P

-

D

T

V

L

E

A

H

D

W

W

T

D

R

A

I

L

N

G

R

H

K

K

V

L

P

P

R

C

L

L

V

V

S

D

V

L

L

Q

P

P

N

S

G

G

P

-

D

-

Y

T

H

H

P

L

T

M

V

E

R

D

A

F

F

Y

L

Y

A

A

G

G

G

G

V

V

P

P

E

A

V

V

M

I

L

R

H

E

L

L

R

G

D

D

L

-

G

-

L

V

L

I

H

A

L

R

D

D

A

A

M

L

T

T

V

V

T

N

G

G

Q

Q

T

T

V

L

G

W

E

D

N

N

L

C

E

K

W

-

W

-

Q

D

A

A

S

P

E

N

R

W

R

N

A

R

R

E

F

V

R

I

Q

H

C

A

L

F

R

N

E

E

Q

-

D

-

G

-

V

-

E

-

P

-

D

-

D

-

V

-

I

-

L

-

P

-

P

P

E

F

K

K

A

T

K

E

A

A

K

G

G

-

L

-

T

-

S

-

T

-

V

I

C

A

F

V

P

L

T

R

G

G

N

N

I

L

A

C

P

P

E

D

G

G

S

A

V

V

I

I

K

K

A

P

T

S

A

A

I

A

D

T

P

P

S

A

V

L

V

L

G

-

E

-

D

-

G

-

V

-

Y

-

H

K

H

H

T

K

G

G

R

R

V

A

R

V

V

V

F

F

V

E

S

N

E

S

A

E

Q

H

A

M

I

H

K

E

A

R

I

M

K

D

R

D

E

E

-

N

-

L

E

D

I

V

V

D

Q

E

G

N

D

C

I

V

M

L

V

V

V

L

I

K

G

N

G

C

G

G

P

P

S

R

G

G

T

Y

-

P

G

G

M

M

E

A

E
|
E

T

A

Y

G

Q

N

L

M

T

P

S

L

A

P

L

P

K

K

H

I

I

L

S

R

W

K

G

G

K

I

T

T

-

D

V

M

S

V

L

R

I

V

T

S

D

D

A

A

R

R

F

M

S
|
S

G

G

V

T

S

A

T

Y

G

G

A

T

C

V

F

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

L

A

A

A

G

G

G

G

P

P

I

L

G

A

K

L

L

V

R

Q

D

D

N

G

D

D

I

I

I

I

E

E

I

L

A

D

V

V

binding bicarbonate ion: W162 (≠ Q202), S470 (= S537)
binding fe2/s2 (inorganic) cluster: C119 (= C162), D120 (= D163), H191 (vs. gap), C192 (= C233)
binding manganese (ii) ion: D120 (= D163), K121 (= K164), E443 (= E511)

Sites not aligning to the query:

8ej0A Dihydroxyacid dehydratase
32% identity, 67% coverage: 132:571/655 of query aligns to 89:503/568 of 8ej0A

query
sites
8ej0A

S

A

L

M

P

L

Y

F

R

R

N

N

D

L

A

A

A

S

I

M

V

D

F

V

R

E

R

E

L

S

I

I

R

R

S

G

L

N

P

P

T

L

R

D

R

-

A

G

V

V

I

V

G

L

V

L

A

M

T

G

C
|
C

D
|
D

K
|
K

G

T

L

T

P

P

A

S

T

L

M

M

I

M

A

G

L

-

A

A

A

A

M

S

H

C

D

D

L

L

P

P

T

T

I

I

L

G

V

V

P

S

G

G

G

G

A

P

T

M

L

L

P

S

P

G

T

K

V

F

-

R

G

G

E

R

D

E

A

L

G

G

K

S

-

G

-

T

-

D

V

V

Q
x
W

T

K

I

M

G

S

A

E

R

E

F

V

A

R

N

A

H

G

E

Q

L

M

S

S

L

Q

Q

E

E

E

A

F

A

F

E

E

L

-

G

-

C

A

R

E

A

S

C

C

A

M

S

H

P

R

G

S

G

H

G

G

-

H

C
|
C

Q

M

F

T

L

M

G
|
G

T
|
T

A

A

G

S

T

T

S

M

Q

A

V

S

V

M

A

V

E

E

A

A

L

L

G

G

L

M

A

S

L

L

P

P

H

G

S

N

A

A

L

A

A

I

P

P

S

A

G

V

Q

D

A

A

V

R

W

R

L

N

E

L

I

L

A

A

R

R

Q

A

S

S

A

G

R

R

A

R

V

I

S

V

E

Q

L

M

D

V

S

K

R

D

G

D

I

L

T

V

T

M

R

S

D

K

I

I

L

L

S

T

D

R

K

Q

A

A

I

F

E

E

N

N

A

A

M

I

V

R

I

V

H

N

A

A

A

A

F

I

G

G

G

G

S

S

T

T

N

N

L

A

L

V

L

I

H

H

I

L

P

L

A

A

I

I

A

A

H

G

A

R

A

I

G

G

C

V

T

D

I

L

P

-

D

T

V

L

E

A

H

D

W

W

T

D

R

A

I

L

N

G

R

H

K

K

V

L

P

P

R

C

L

L

V

V

S

D

V

L

L

Q

P

P

N

S

G

G

P

-

D

-

Y

T

H

H

P

L

T

M

V

E

R

D

A

F

F

Y

L

Y

A

A

G

G

G

G

V

V

P

P

E

A

V

V

M

I

L

R

H

E

L

L

R

G

D

D

L

-

G

-

L

V

L

I

H

A

L

R

D

D

A

A

M

L

T

T

V

V

T

N

G

G

Q

Q

T

T

V

L

G

W

E

D

N

N

L

C

E

K

W

-

W

-

Q

D

A

A

S

P

E

N

R

W

R

N

A

R

R

E

F

V

R

I

Q

H

C

A

L

F

R

N

E

E

Q

-

D

-

G

-

V

-

E

-

P

-

D

-

D

-

V

-

I

-

L

-

P

-

P

P

E

F

K

K

A

T

K

E

A

A

K

G

G

-

L

-

T

-

S

-

T

-

V

I

C

A

F

V

P

L

T

R

G

G

N

N

I

L

A

C

P

P

E

D

G

G

S

A

V

V

I

I

K

K

A

P

T

S

A

A

I

A

D

T

P

P

S

A

V

L

V

L

G

-

E

-

D

-

G

-

V

-

Y

-

H

K

H

H

T

K

G

G

R

R

V

A

R

V

V

V

F

F

V

E

S

N

E

S

A

E

Q

H

A

M

I

H

K

E

A

R

I

M

K

D

R

D

E

E

-

N

-

L

E

D

I

V

V

D

Q

E

G

N

D

C

I

V

M

L

V

V

V

L

I

K

G

N

G

C

G

G

P

P

S

R

G

G

T

Y

-

P

G

G

M

M

E

A

E
|
E

T

A

Y

G

Q

N

L

M

T

P

S

L

A

P

L

P

K

K

H

I

I

L

S

R

W

K

G

G

K

I

T

T

-

D

V

M

S

V

L

R

I

V

T

S

D

D

A

A

R

R

F

M

S
|
S

G
|
G

V
x
T

S

A

T

Y

G

G

A

T

C

V

F

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

L

A

A

A

G

G

G

G

P

P

I

L

G

A

K

L

L

V

R

Q

D

D

N

G

D

D

I

I

I

I

E

E

I

L

A

D

V

V

binding bicarbonate ion: D119 (= D163), K120 (= K164), W161 (≠ Q202), G195 (= G237), T196 (= T238), E442 (= E511), S469 (= S537), S469 (= S537), G470 (= G538), T471 (≠ V539)
binding fe2/s2 (inorganic) cluster: C118 (= C162), D119 (= D163), C191 (= C233)
binding magnesium ion: D119 (= D163), K120 (= K164), E442 (= E511)

Sites not aligning to the query:

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
29% identity, 75% coverage: 136:625/655 of query aligns to 96:558/587 of 7m3kA

query
sites
7m3kA

R

R

N

N

D

L

A

A

A

Y

I

L

V

G

F

L

R

V

R

E

L

V

I

L

R

Y

S

G

L

Y

P

P

T

L

R

D

R

G

A

V

V

V

I

L

G

T

V

I

A

G

T

-

C
|
C

D

D

K

K

G

T

L

T

P

P

A

A

T

C

M

L

I

M

A

A

L

-

A

A

A

A

M

T

H

V

D

N

L

I

P

P

T

A

I

I

L

A

V

L

P

S

G

V

G

G

A

P

T

M

L

L

P

N

P

G

T

W

V

F

-

R

G

G

E

E

D

R

A

T

G

G

K

S

V

G

Q

T

T

I

I

V

-

W

G

K

A

A

R

R

-

E

-

L

F

L

A

A

N

K

H

G

E

E

L

I

S

D

L

Y

Q

Q

E

G

A

F

A

V

E

K

L

L

G

-

C

V

R

A

A

S

C

S

A

A

S

P

P

S

G

T

G

G

G

Y

C

C

Q

N

F

T

L

M

G

G

T

T

A

A

G

T

T

T

S

M

Q

N

V

S

V

L

A

A

E

E

A

A

L

L

G

G

L

M

A

Q

L

L

P

P

H

G

S

S

A

A

L

A

A

I

P

P

S

A

G

P

Q

Y

A

R

V

D

W

R

L

Q

E

E

I

V

A

A

R

Y

Q

L

S

M

A

G

R

R

A

R

V

I

S

V

E

E

L

M

D

V

S

H

R

E

G

D

I

L

T

K

T

P

R

S

D

D

I

I

L

L