SitesBLAST
Comparing 15560 FitnessBrowser__Keio:15560 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
26% identity, 80% coverage: 95:468/468 of query aligns to 24:402/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: G40 (≠ A111), L41 (≠ F112)
- binding 2-oxoglutaric acid: D213 (= D280), P214 (≠ V281), Y215 (= Y282), G216 (≠ S283), E217 (= E284), G241 (≠ S308), T242 (≠ S309), I246 (≠ C313)
- binding (2E)-pent-2-enedioic acid: G40 (≠ A111), Y130 (≠ F201), N184 (= N251), R376 (= R442)
- binding glutamic acid: L131 (≠ Y202), V360 (≠ A426), A364 (≠ M430), R369 (≠ E435)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G175), S105 (≠ A176), Q106 (≠ L177), Y130 (≠ F201), N184 (= N251), D212 (= D279), P214 (≠ V281), Y215 (= Y282), T242 (≠ S309), S244 (= S311), K245 (= K312), R252 (= R319)
Sites not aligning to the query:
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
26% identity, 80% coverage: 95:468/468 of query aligns to 24:402/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: L131 (≠ Y202), Q135 (= Q206), A364 (≠ M430), R369 (≠ E435)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: G40 (≠ A111), Y130 (≠ F201), L131 (≠ Y202), A132 (≠ G203), N184 (= N251), R376 (= R442)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G175), S105 (≠ A176), Q106 (≠ L177), Y130 (≠ F201), V179 (≠ M246), N184 (= N251), D212 (= D279), P214 (≠ V281), Y215 (= Y282), T242 (≠ S309), S244 (= S311), K245 (= K312), R252 (= R319)
Sites not aligning to the query:
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
26% identity, 80% coverage: 95:468/468 of query aligns to 24:402/404 of 3aowC
- binding 2-oxoglutaric acid: Y70 (≠ M137), Y130 (≠ F201), L275 (= L341)
- binding pyridoxal-5'-phosphate: G104 (= G175), S105 (≠ A176), Q106 (≠ L177), Y130 (≠ F201), V179 (≠ M246), N184 (= N251), D212 (= D279), P214 (≠ V281), Y215 (= Y282), T242 (≠ S309), S244 (= S311), K245 (= K312), R252 (= R319)
Sites not aligning to the query:
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
26% identity, 80% coverage: 95:468/468 of query aligns to 24:402/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G175), S105 (≠ A176), Q106 (≠ L177), Y130 (≠ F201), V179 (≠ M246), N184 (= N251), D212 (= D279), P214 (≠ V281), Y215 (= Y282), T242 (≠ S309), S244 (= S311), K245 (= K312), R252 (= R319)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
29% identity, 80% coverage: 95:468/468 of query aligns to 21:400/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G175), S103 (≠ A176), Q104 (≠ L177), Y128 (≠ F201), V177 (≠ M246), N182 (= N251), D210 (= D279), P212 (≠ V281), Y213 (= Y282), T240 (≠ S309), S242 (= S311), K243 (= K312), R250 (= R319)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
30% identity, 80% coverage: 91:465/468 of query aligns to 20:391/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I22 (= I93), R23 (≠ F94), G39 (≠ S110), G40 (≠ A111), G99 (= G175), S100 (≠ A176), Q101 (≠ L177), Y125 (≠ F201), N174 (= N251), D202 (= D279), Y205 (= Y282), S235 (= S309), S237 (= S311), K238 (= K312), R245 (= R319), R368 (= R442)
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
30% identity, 80% coverage: 91:465/468 of query aligns to 20:391/397 of Q72LL6
- S20 (≠ T91) mutation to E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- R23 (≠ F94) mutation to A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; mutation to Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
- G40 (≠ A111) binding
- Y70 (≠ E141) binding
- N174 (= N251) binding ; binding
- R245 (= R319) binding
- R368 (= R442) binding
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
30% identity, 80% coverage: 91:465/468 of query aligns to 16:387/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: I18 (= I93), R19 (≠ F94), G35 (≠ S110), G36 (≠ A111), G95 (= G175), S96 (≠ A176), Q97 (≠ L177), Y121 (≠ F201), N170 (= N251), D198 (= D279), Y201 (= Y282), S231 (= S309), S233 (= S311), K234 (= K312), R241 (= R319), R364 (= R442)
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
30% identity, 80% coverage: 91:465/468 of query aligns to 16:387/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G175), S96 (≠ A176), Q97 (≠ L177), Y121 (≠ F201), N170 (= N251), D198 (= D279), A200 (≠ V281), Y201 (= Y282), S231 (= S309), S233 (= S311), K234 (= K312), R241 (= R319)
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
31% identity, 77% coverage: 104:465/468 of query aligns to 25:383/389 of 2z1yA
- binding leucine: G32 (≠ A111), Y117 (≠ F201), R360 (= R442)
- binding pyridoxal-5'-phosphate: G91 (= G175), S92 (≠ A176), Q93 (≠ L177), Y117 (≠ F201), N166 (= N251), D194 (= D279), Y197 (= Y282), S227 (= S309), S229 (= S311), K230 (= K312), R237 (= R319)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
28% identity, 78% coverage: 104:468/468 of query aligns to 35:405/405 of 2zc0A
- active site: Y132 (≠ F201), D214 (= D279), A216 (≠ V281), S246 (= S311)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G106 (= G175), G107 (≠ A176), T108 (≠ L177), Y132 (≠ F201), N186 (= N251), D214 (= D279), A216 (≠ V281), Y217 (= Y282), T244 (≠ S309), S246 (= S311), K247 (= K312), R254 (= R319)
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
27% identity, 81% coverage: 90:466/468 of query aligns to 25:412/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G175), S113 (≠ A176), Q114 (≠ L177), Y138 (≠ F201), N194 (= N251), D222 (= D279), P224 (≠ V281), Y225 (= Y282), T252 (≠ S309), S254 (= S311), K255 (= K312), R262 (= R319)
8tn3A Structure of s. Hygroscopicus aminotransferase mppq complexed with pyridoxamine 5'-phosphate (pmp) (see paper)
28% identity, 76% coverage: 104:460/468 of query aligns to 20:383/388 of 8tn3A
2vgzA Crystal structure of human kynurenine aminotransferase ii (see paper)
25% identity, 78% coverage: 103:468/468 of query aligns to 20:414/414 of 2vgzA
2xh1A Crystal structure of human kat ii-inhibitor complex (see paper)
26% identity, 79% coverage: 99:468/468 of query aligns to 12:412/412 of 2xh1A
- binding (3s)-10-(4-aminopiperazin-1-yl)-9-fluoro-7-hydroxy-3-methyl-2,3-dihydro-8h-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylate: G26 (≠ A111), L27 (≠ F112), Y61 (≠ M137), L280 (= L341)
- binding pyridoxal-5'-phosphate: S104 (≠ A176), Q105 (≠ L177), Y129 (≠ F201), D217 (= D279), P219 (≠ V281), S247 (= S309), S249 (= S311), R257 (= R319)
5tf5A Crystal structure of human kat-2 in complex with a reversible inhibitor
26% identity, 79% coverage: 100:468/468 of query aligns to 27:425/425 of 5tf5A
Sites not aligning to the query:
2r2nA The crystal structure of human kynurenine aminotransferase ii in complex with kynurenine (see paper)
26% identity, 79% coverage: 100:468/468 of query aligns to 27:425/425 of 2r2nA
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: Y142 (≠ F201), R399 (= R442)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (≠ A176), Q118 (≠ L177), Y142 (≠ F201), N202 (= N251), D230 (= D279), P232 (≠ V281), Y233 (= Y282), S260 (= S309), S262 (= S311), K263 (= K312), R270 (= R319)
Sites not aligning to the query:
6t8qA Hkatii in complex with ligand (2r)-n-benzyl-1-[6-methyl-5-(oxan-4-yl)- 7-oxo-6h,7h-[1,3]thiazolo[5,4-d]pyrimidin-2-yl]pyrrolidine-2- carboxamide (see paper)
26% identity, 79% coverage: 100:468/468 of query aligns to 30:428/428 of 6t8qA
4gebA Kynurenine aminotransferase ii inhibitors (see paper)
26% identity, 79% coverage: 100:468/468 of query aligns to 27:425/428 of 4gebA
- binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G39 (≠ A111), L40 (≠ F112), Y74 (≠ M137), S117 (≠ A176), Q118 (≠ L177), Y142 (≠ F201), N202 (= N251), D230 (= D279), P232 (≠ V281), Y233 (= Y282), S260 (= S309), S262 (= S311), K263 (= K312), R270 (= R319), R399 (= R442)
4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
26% identity, 79% coverage: 100:468/468 of query aligns to 27:425/428 of 4ge9A
- binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G39 (≠ A111), L40 (≠ F112), Y74 (≠ M137), S75 (= S138), S77 (≠ I140), S117 (≠ A176), Q118 (≠ L177), Y142 (≠ F201), N202 (= N251), D230 (= D279), P232 (≠ V281), Y233 (= Y282), S260 (= S309), S262 (= S311), R270 (= R319), L293 (= L341), R399 (= R442)
Sites not aligning to the query:
Query Sequence
>15560 FitnessBrowser__Keio:15560
MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVSHAYQLLESQGYIIARP
QSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRDPSVVPFASAFPDPRLFPL
QQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYALQGITISPDEIVITAGALEAL
NLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSVATDVKEGIDLQALELALQEYPV
KACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTLIEDDVYSELYFGREKPLPAKAWDR
HDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQRLQLMSTLSTSSPMQLALVDYLSTRRY
DAHLRRLRRQLAERKQRAWQALLRYLPAEVKIHHNDSGYFLWLELPEPLDAGELSLAALT
HHISIAPGKMFSTGENWSRFFRFNTAWQWGEREEQAVKQLGKLIQERL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory