SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16033 FitnessBrowser__Keio:16033 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4d8uH Crystal structure of d-cysteine desulfhydrase from salmonella typhimurium at 3.3 a in monoclinic space group with 8 subunits in the asymmetric unit (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 4:331/331 of 4d8uH

query
sites
4d8uH

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active site: K54 (= K51), Y264 (= Y261), Y290 (= Y287)
binding phosphate ion: K54 (= K51), S81 (= S78), N82 (= N79), H83 (= H80), G162 (= G159), Y264 (= Y261), Y290 (= Y287)

4d9fA D-cysteine desulfhydrase from salmonella typhimurium complexed with d- cycloserine (dcs) (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d9fA

query
sites
4d9fA

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active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), V158 (= V158), G159 (= G159), S161 (= S161), S193 (= S193), G194 (= G194), S195 (= S195), A196 (= A196), G197 (= G197), T198 (= T198), Y287 (= Y287), T315 (= T315), G316 (= G316), G317 (= G317)

4d9eA D-cysteine desulfhydrase from salmonella typhimurium complexed with l- cycloserine (lcs) (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d9eA

query
sites
4d9eA

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active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: N50 (= N50), K51 (= K51), K54 (= K54), S78 (= S78), N79 (= N79), H80 (= H80), G159 (= G159), G194 (= G194), S195 (= S195), A196 (= A196), G197 (= G197), T198 (= T198), Y287 (= Y287), T315 (= T315), G316 (= G316), G317 (= G317)

4d9bA Pyridoxamine 5' phosphate (pmp) bound form of salmonella typhimurium d-cysteine desulfhydrase obtained after co-crystallization with d- cycloserine (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d9bA

query
sites
4d9bA

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L

E

E

G

G

I

V

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

A

A

G

G

L

L

I

I

D

D

G

G

I

I

S

S

Q

Q

K

K

R

R

F

F

K

N

D

D

E

D

G

G

P

P

I

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

A

A

P

P

A

A

L

L

F

F

A

A

Y

Y

H

H

P

P

H

H

V

V

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), H80 (= H80), S161 (= S161), A192 (= A192), S193 (= S193), G194 (= G194), S195 (= S195), A196 (= A196), G197 (= G197), T198 (= T198), Y287 (= Y287), T315 (= T315), G316 (= G316), G317 (= G317)

4d99A Salmonella typhimurium d-cysteine desulfhydrase with l-ser bound non- covalently at the active site (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d99A

query
sites
4d99A

M

M

P

P

L

L

H

H

N

H

L

L

T

T

R

R

F

F

P

P

R

R

L

L

E

E

F

F

I

I

G

G

A

A

P

P

T

T

P

P

L

L

E

E

Y

Y

L

L

P

P

R

R

F

L

S

S

D

D

Y

Y

L

L

G

G

R

R

E

E

I

I

F

Y

I

I

K

K

R

R

D

D

V

V

T

T

P

P

M

I

A

A

M

M

G

G

N

N

K
|
K

L

L

R

R

K

K

L

L

E

E

F

F

L

L

A

V

A

A

D

D

A

A

L

L

R

R

E

E

G

G

A

A

D

D

T

T

L

L

I

I

T

T

A

A

G
|
G

A
|
A

I

I

Q

Q

S
|
S

N
|
N

H
|
H

V

V

R

R

Q

Q

T

T

A

A

V

V

A

A

K

K

L

L

G

G

L

L

H

H

C

C

V

V

A

A

L

L

E

E

N

N

P

P

I

I

G

G

T

T

A

A

E

E

N

N

Y

Y

L

L

T

T

N

N

G

G

N

N

R

R

L

L

D

D

L

L

F

F

N

N

T

T

Q

Q

I

I

E

E

M

M

C

C

D

D

A

A

L

L

T

T

D

D

P

P

N

D

A

A

Q

Q

L

L

E

Q

E

T

L

L

A

A

T

T

R

R

V

I

E

E

A

A

Q

Q

G

G

F

F

R

R

P

P

Y

Y

V

V

I

I

P

P

V

V

G
|
G

G

G

S

S

N

S

A

A

L

L

G

G

A

A

L

M

G

G

Y

Y

V

V

E

E

S

S

A

A

L

L

E

E

I

I

A

A

Q

Q

C

C

E

E

G

E

A

V

V

V

N

G

I

L

S

S

V

V

A

A

S

S

G

G

S

S

A

A

G

G

T

T

H

H

A

A

G

G

L

L

A

A

V

V

G

G

L

L

E

E

H

H

L

L

M

M

P

P

E

D

S

V

E

E

L

L

I

I

G

G

V

V

T

T

V

V

S

S

R

R

S

S

V

V

A

A

D

E

Q

Q

L

K

P

P

K

K

V

V

V

I

N

A

L

L

Q

Q

A

A

I

I

A

A

K

G

E

Q

L

L

E

A

L

L

T

T

A

A

S

T

A

A

E

D

I

I

L

H

L

L

W

W

D

D

Y

Y

F

F

A

A

P

P

G

G

Y
|
Y

G

G

V

V

P

P

N

N

D

D

E

A

G

G

M

M

E

E

A

A

V

V

K

K

L

L

A

A

R

S

L

L

E

E

G

G

I

V

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

A

A

G

G

L

L

I

I

D

D

G

G

I

I

S

S

Q

Q

K

K

R

R

F

F

K

N

D

D

E

D

G

G

P

P

I

I

L

L

F

F

I

I

H

H

T

T

G

G

A

A

P

P

A

A

L

L

F

F

A

A

Y

Y

H

H

P

P

H

H

V

V

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding serine: K51 (= K51), G74 (= G74), A75 (= A75), S78 (= S78), N79 (= N79), H80 (= H80), G159 (= G159), Y261 (= Y261), Y287 (= Y287)

4d97A Salmonella typhimurium d-cysteine desulfhydrase with d-ser bound at active site (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d97A

query
sites
4d97A

M

M

P

P

L

L

H

H

N

H

L

L

T

T

R

R

F

F

P

P

R

R

L

L

E

E

F

F

I

I

G

G

A

A

P

P

T

T

P

P

L

L

E

E

Y

Y

L

L

P

P

R

R

F

L

S

S

D

D

Y

Y

L

L

G

G

R

R

E

E

I

I

F

Y

I

I

K

K

R

R

D

D

V

V

T

T

P

P

M

I

A

A

M

M

G

G

N

N

K
|
K

L

L

R

R

K

K

L

L

E

E

F

F

L

L

A

V

A

A

D

D

A

A

L

L

R

R

E

E

G

G

A

A

D

D

T

T

L

L

I

I

T

T

A
|
A

G
|
G

A

A

I

I

Q

Q

S
|
S

N
|
N

H
|
H

V

V

R

R

Q

Q

T

T

A

A

V

V

A

A

K

K

L

L

G

G

L

L

H

H

C

C

V

V

A

A

L

L

E

E

N

N

P

P

I

I

G

G

T

T

A

A

E

E

N

N

Y

Y

L

L

T

T

N

N

G

G

N

N

R

R

L

L

D

D

L

L

F

F

N

N

T

T

Q

Q

I

I

E

E

M

M

C

C

D

D

A

A

L

L

T

T

D

D

P

P

N

D

A

A

Q

Q

L

L

E

Q

E

T

L

L

A

A

T

T

R

R

V

I

E

E

A

A

Q

Q

G

G

F

F

R

R

P

P

Y

Y

V

V

I

I

P

P

V
|
V

G
|
G

G

G

S

S

N

S

A

A

L

L

G

G

A

A

L

M

G

G

Y

Y

V

V

E

E

S

S

A

A

L

L

E

E

I

I

A

A

Q

Q

C

C

E

E

G

E

A

V

V

V

N

G

I

L

S

S

V

V

A

A

S

S

G

G

S

S

A

A

G

G

T

T

H

H

A

A

G

G

L

L

A

A

V

V

G

G

L

L

E

E

H

H

L

L

M

M

P

P

E

D

S

V

E

E

L

L

I

I

G

G

V

V

T

T

V

V

S

S

R

R

S

S

V

V

A

A

D

E

Q

Q

L

K

P

P

K

K

V

V

V

I

N

A

L

L

Q

Q

A

A

I

I

A

A

K

G

E

Q

L

L

E

A

L

L

T

T

A

A

S

T

A

A

E

D

I

I

L

H

L

L

W

W

D

D

Y

Y

F

F

A

A

P

P

G

G

Y
|
Y

G

G

V

V

P

P

N

N

D

D

E

A

G

G

M

M

E

E

A

A

V

V

K

K

L

L

A

A

R

S

L

L

E

E

G

G

I

V

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

A

A

G

G

L

L

I

I

D

D

G

G

I

I

S

S

Q

Q

K

K

R

R

F

F

K

N

D

D

E

D

G

G

P

P

I

I

L

L

F

F

I

I

H

H

T

T

G

G

A

A

P

P

A

A

L

L

F

F

A

A

Y

Y

H

H

P

P

H

H

V

V

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding d-serine: K51 (= K51), A73 (= A73), G74 (= G74), S78 (= S78), N79 (= N79), H80 (= H80), V158 (= V158), G159 (= G159), Y287 (= Y287)

4d96A D-cysteine desulfhydrase from salmonella typhimurium complexed with 1- amino-1-carboxycyclopropane (acc) (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d96A

query
sites
4d96A

M

M

P

P

L

L

H

H

N

H

L

L

T

T

R

R

F

F

P

P

R

R

L

L

E

E

F

F

I

I

G

G

A

A

P

P

T

T

P

P

L

L

E

E

Y

Y

L

L

P

P

R

R

F

L

S

S

D

D

Y

Y

L

L

G

G

R

R

E

E

I

I

F

Y

I

I

K

K

R

R

D

D

V

V

T

T

P

P

M

I

A

A

M

M

G

G

N
|
N

K
|
K

L

L

R

R

K
|
K

L

L

E

E

F

F

L

L

A

V

A

A

D

D

A

A

L

L

R

R

E

E

G

G

A

A

D

D

T

T

L

L

I

I

T

T

A

A

G

G

A

A

I

I

Q

Q

S
|
S

N
|
N

H
|
H

V

V

R

R

Q

Q

T

T

A

A

V

V

A

A

K

K

L

L

G

G

L

L

H

H

C

C

V

V

A

A

L

L

E

E

N

N

P

P

I

I

G

G

T

T

A

A

E

E

N

N

Y

Y

L

L

T

T

N

N

G

G

N

N

R

R

L

L

D

D

L

L

F

F

N

N

T

T

Q

Q

I

I

E

E

M

M

C

C

D

D

A

A

L

L

T

T

D

D

P

P

N

D

A

A

Q

Q

L

L

E

Q

E

T

L

L

A

A

T

T

R

R

V

I

E

E

A

A

Q

Q

G

G

F

F

R

R

P

P

Y

Y

V

V

I

I

P

P

V

V

G
|
G

G

G

S
|
S

N

S

A

A

L

L

G

G

A

A

L

M

G

G

Y

Y

V

V

E

E

S

S

A

A

L

L

E

E

I

I

A

A

Q

Q

C

C

E

E

G

E

A

V

V

V

N

G

I

L

S

S

V

V

A
|
A

S
|
S

G
|
G

S
|
S

A
|
A

G
|
G

T
|
T

H

H

A

A

G

G

L

L

A

A

V

V

G

G

L

L

E

E

H

H

L

L

M

M

P

P

E

D

S

V

E

E

L

L

I

I

G

G

V

V

T

T

V

V

S

S

R

R

S

S

V

V

A

A

D

E

Q

Q

L

K

P

P

K

K

V

V

V

I

N

A

L

L

Q

Q

A

A

I

I

A

A

K

G

E

Q

L

L

E

A

L

L

T

T

A

A

S

T

A

A

E

D

I

I

L

H

L

L

W

W

D

D

Y

Y

F

F

A

A

P

P

G

G

Y
|
Y

G

G

V

V

P

P

N

N

D

D

E

A

G

G

M

M

E

E

A

A

V

V

K

K

L

L

A

A

R

S

L

L

E

E

G

G

I

V

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

A

A

G

G

L

L

I

I

D

D

G

G

I

I

S

S

Q

Q

K

K

R

R

F

F

K

N

D

D

E

D

G

G

P

P

I

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

A

A

P

P

A

A

L

L

F

F

A

A

Y

Y

H

H

P

P

H

H

V

V

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N50 (= N50), K51 (= K51), K54 (= K54), S78 (= S78), N79 (= N79), H80 (= H80), G159 (= G159), S161 (= S161), A192 (= A192), S193 (= S193), G194 (= G194), S195 (= S195), A196 (= A196), G197 (= G197), T198 (= T198), Y287 (= Y287), T315 (= T315), G316 (= G316), G317 (= G317)

4d8wA Salmonella typhimurium d-cysteine desulfhydrase soaked with d-cys shows pyruvate bound 4 a away from active site (see paper)
90% identity, 100% coverage: 1:328/328 of query aligns to 1:328/328 of 4d8wA

query
sites
4d8wA

M

M

P

P

L

L

H

H

N

H

L

L

T

T

R

R

F

F

P

P

R

R

L

L

E

E

F

F

I

I

G

G

A

A

P

P

T

T

P

P

L

L

E

E

Y

Y

L

L

P

P

R

R

F

L

S

S

D

D

Y

Y

L

L

G

G

R

R

E

E

I

I

F

Y

I

I

K

K

R

R

D

D

V

V

T

T

P

P

M

I

A

A

M

M

G

G

N

N

K
|
K

L

L

R

R

K

K

L

L

E

E

F

F

L

L

A

V

A

A

D

D

A

A

L

L

R

R

E

E

G

G

A

A

D

D

T

T

L

L

I

I

T

T

A

A

G

G

A

A

I

I

Q

Q

S

S

N

N

H

H

V

V

R

R

Q

Q

T

T

A

A

V

V

A

A

K

K

L

L

G

G

L

L

H

H

C

C

V

V

A

A

L

L

E

E

N

N

P

P

I

I

G

G

T

T

A

A

E

E

N

N

Y

Y

L

L

T

T

N

N

G

G

N

N

R

R

L

L

D

D

L

L

F

F

N

N

T

T

Q

Q

I

I

E

E

M

M

C

C

D

D

A

A

L

L

T

T

D

D

P

P

N

D

A

A

Q

Q

L

L

E

Q

E

T

L

L

A

A

T

T

R

R

V

I

E

E

A

A

Q

Q

G

G

F

F

R

R

P

P

Y

Y

V

V

I

I

P

P

V

V

G

G

S

S

N

S

A

A

L

L

G

G

A

A

L

M

G

G

Y

Y

V

V

E

E

S

S

A

A

L

L

E

E

I

I

A

A

Q

Q

C

C

E

E

G

E

A

V

V

V

N

G

I

L

S

S

V

V

A

A

S

S

G
|
G

S
|
S

A

A

G

G

T

T

H

H

A

A

G

G

L

L

A

A

V

V

G

G

L

L

E

E

H

H

L

L

M

M

P

P

E

D

S

V

E

E

L

L

I

I

G

G

V

V

T

T

V

V

S
|
S

R
|
R

S

S

V

V

A

A

D

E

Q

Q

L

K

P

P

K

K

V

V

V

I

N

A

L

L

Q

Q

A

A

I

I

A

A

K

G

E

Q

L

L

E

A

L

L

T

T

A

A

S

T

A

A

E

D

I

I

L

H

L

L

W

W

D

D

Y

Y

F

F

A

A

P

P

G

G

Y
|
Y

G

G

V

V

P

P

N

N

D

D

E

A

G

G

M

M

E

E

A

A

V

V

K

K

L

L

A

A

R

S

L

L

E

E

G

G

I

V

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

A

A

G

G

L

L

I

I

D

D

G

G

I

I

S

S

Q

Q

K

K

R

R

F

F

K

N

D

D

E

D

G

G

P

P

I

I

L

L

F

F

I

I

H

H

T

T

G

G

A

A

P

P

A

A

L

L

F

F

A

A

Y

Y

H

H

P

P

H

H

V

V

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding pyruvic acid: G194 (= G194), S195 (= S195), S221 (= S221), R222 (= R222), Y261 (= Y261), Y287 (= Y287)

1j0bA Crystal structure analysis of the acc deaminase homologue complexed with inhibitor (see paper)
40% identity, 97% coverage: 6:324/328 of query aligns to 9:317/325 of 1j0bA

query
sites
1j0bA

L

L

T

A

R

K

F

F

P

P

R

R

L

V

E

E

F

L

I

I

G

P

A

W

P

E

T

T

P

P

L

I

E

Q

Y

Y

L

L

P

P

R

N

F

I

S

S

D

R

Y

E

L

I

G

G

R

A

E

D

I

V

F

Y

I

I

K

K

R

R

D

D

V

L

T

T

P

G

M

L

A

G

M

I

G

G

N
|
N

K
|
K

L

I

R

R

K
|
K

L

L

E

E

F

Y

L

L

A

L

A

G

D

D

A

A

L

L

R

S

E

K

G

G

A

A

D

D

T

V

L

V

I

I

T

T

A

V

G

G

A

A

I

V

Q

H

S
|
S

N
|
N

H
|
H

V

A

R

F

Q

V

T

T

A

G

A

L

V

A

A

A

A

K

K

K

L

L

G

G

L

L

H

D

C

A

V

I

A

L

L

V

L

L

E

R

N

G

P

-

I

-

G

-

T

-

A

-

E

-

N

K

Y

E

L

E

T

L

N

K

G

G

N

N

R

Y

L

L

L

D

D

K

L

I

F

M

N

G

T

I

Q

E

I

T

E

R

M

V

C

Y

D

D

A

A

L

K

T

D

-

S

-

F

D

E

P

L

N

M

A

K

Q

Y

L

A

E

E

L

I

A

A

T

E

R

E

V

L

E

K

A

R

Q

E

G

G

F

R

R

K

P

P

Y

Y

V

V

I

I

P

P

V

P

G
|
G

G

G

S

A

N

S

A

P

L

I

G

G

A

T

L

L

G

G

Y

Y

V

V

E

R

S

A

A

V

L

G

E

E

I

I

A

A

Q

T

Q

Q

C

S

E

E

G

-

A

-

V

V

N

K

I

F

S

D

S

S

V

I

V

V

A
|
A

S
x
A

G
|
G

S
|
S

A
x
G

G
|
G

T
|
T

H

L

A

A

G

G

L

L

A

S

V

L

G

G

L

L

E

S

H

I

L

L

M

N

P

E

E

D

S

I

E

R

L

P

I

V

G

G

V

I

T

A

V

V

S

G

R

R

S

F

V

G

A

E

D

V

Q

M

L

T

P

S

K

K

V

L

V

D

N

N

L

L

Q

I

Q

K

A

E

I

A

A

A

K

E

E

L

L

L

E

G

L

V

T

K

A

V

S

E

A

V

E

R

I

P

L

E

L

L

W

Y

D

D

D

Y

Y

S

F

F

A

G

P

E

G

-

Y
|
Y

G

G

V

K

P

I

N

T

D

G

E

E

G

V

M

A

E

Q

A

I

V

I

K

R

L

K

L

V

A

G

R

T

L

R

E

E

G

G

I

I

L

I

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

F

A

Y

G

G

L

L

I

V

D

D

G

L

I

A

S

R

Q

K

K

G

R

E

F

L

K

G

D

E

E

K

G

-

P

-

I

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

A

I

P

S

A

G

L

T

F

F

A

H

Y

Y

active site: K54 (= K51), Y256 (= Y261), Y282 (= Y287)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N53 (= N50), K54 (= K51), K57 (= K54), S81 (= S78), N82 (= N79), H83 (= H80), G157 (= G159), A188 (= A192), A189 (≠ S193), G190 (= G194), S191 (= S195), G192 (≠ A196), G193 (= G197), T194 (= T198), Y282 (= Y287), T308 (= T315), G309 (= G316), G310 (= G317)

1j0aA Crystal structure analysis of the acc deaminase homologue (see paper)
40% identity, 97% coverage: 6:324/328 of query aligns to 9:317/325 of 1j0aA

query
sites
1j0aA

L

L

T

A

R

K

F

F

P

P

R

R

L

V

E

E

F

L

I

I

G

P

A

W

P

E

T

T

P

P

L

I

E

Q

Y

Y

L

L

P

P

R

N

F

I

S

S

D

R

Y

E

L

I

G

G

R

A

E

D

I

V

F

Y

I

I

K

K

R

R

D

D

V

L

T

T

P

G

M

L

A

G

M

I

G

G

N
|
N

K
|
K

L

I

R

R

K
|
K

L

L

E

E

F

Y

L

L

A

L

A

G

D

D

A

A

L

L

R

S

E

K

G

G

A

A

D

D

T

V

L

V

I

I

T

T

A

V

G

G

A

A

I

V

Q

H

S

S

N
|
N

H

H

V

A

R

F

Q

V

T

T

A

G

A

L

V

A

A

A

A

K

K

K

L

L

G

G

L

L

H

D

C

A

V

I

A

L

L

V

L

L

E

R

N

G

P

-

I

-

G

-

T

-

A

-

E

-

N

K

Y

E

L

E

T

L

N

K

G

G

N

N

R

Y

L

L

L

D

D

K

L

I

F

M

N

G

T

I

Q

E

I

T

E

R

M

V

C

Y

D

D

A

A

L

K

T

D

-

S

-

F

D

E

P

L

N

M

A

K

Q

Y

L

A

E

E

L

I

A

A

T

E

R

E

V

L

E

K

A

R

Q

E

G

G

F

R

R

K

P

P

Y

Y

V

V

I

I

P

P

V

P

G

G

S

A

N

S

A

P

L

I

G

G

A

T

L

L

G

G

Y

Y

V

V

E

R

S

A

A

V

L

G

E

E

I

I

A

A

Q

T

Q

Q

C

S

E

E

G

-

A

-

V

V

N

K

I

F

S

D

S

S

V

I

V

V

A
|
A

S
x
A

G
|
G

S
|
S

A
x
G

G
|
G

T
|
T

H

L

A

A

G

G

L

L

A

S

V

L

G

G

L

L

E

S

H

I

L

L

M

N

P

E

E

D

S

I

E

R

L

P

I

V

G

G

V

I

T

A

V

V

S

G

R

R

S

F

V

G

A

E

D

V

Q

M

L

T

P

S

K

K

V

L

V

D

N

N

L

L

Q

I

Q

K

A

E

I

A

A

A

K

E

E

L

L

L

E

G

L

V

T

K

A

V

S

E

A

V

E

R

I

P

L

E

L

L

W

Y

D

D

D

Y

Y

S

F

F

A

G

P

E

G

-

Y
|
Y

G

G

V

K

P

I

N

T

D

G

E

E

G

V

M

A

E

Q

A

I

V

I

K

R

L

K

L

V

A

G

R

T

L

R

E

E

G

G

I

I

L

I

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

F

A

Y

G

G

L

L

I

V

D

D

G

L

I

A

S

R

Q

K

K

G

R

E

F

L

K

G

D

E

E

K

G

-

P

-

I

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

A

I

P

S

A

G

L

T

F

F

A

H

Y

Y

active site: K54 (= K51), Y256 (= Y261), Y282 (= Y287)
binding pyridoxal-5'-phosphate: N53 (= N50), K54 (= K51), K57 (= K54), N82 (= N79), A188 (= A192), A189 (≠ S193), G190 (= G194), S191 (= S195), G192 (≠ A196), G193 (= G197), T194 (= T198), Y282 (= Y287), T308 (= T315), G309 (= G316), G310 (= G317)

1tzmA Crystal structure of acc deaminase complexed with substrate analog b- chloro-d-alanine (see paper)
38% identity, 98% coverage: 5:324/328 of query aligns to 2:324/331 of 1tzmA

query
sites
1tzmA

N

N

L

L

T

Q

R

R

F

F

P

P

R

R

L

Y

E

P

F

L

I

T

G

F

A

G

P

P

T

T

P

P

L

I

E

Q

Y

P

L

L

P

A

R

R

F

L

S

S

D

K

Y

H

L

L

G

G

R

G

E

K

I

V

-

H

-

L

F

Y

I

A

K

K

R

R

D

E

D

D

V

C

T

N

P

S

-

G

M

L

A

A

M

F

G

G

N
|
N

K
|
K

L

T

R

R

K
|
K

L

L

E

E

F

Y

L

L

A

I

A

P

D

E

A

A

L

L

R

A

E

Q

G

G

A

C

D

D

T

T

L

L

I

V

T

S

A
x
I

G
|
G

A

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

V

T

R

R

Q

Q

T

V

A

A

V

V

A

A

K

H

L

L

G

G

L

M

H

K

C

C

V

V

A

L

L

V

L

Q

E

E

N

N

P
x
W

I

V

G

N

T

Y

T

S

A

D

E

A

N

V

Y

Y

L

D

T

R

N

V

G

G

N

N

R

I

L

Q

L

M

L

S

D

R

L

I

F

L

N

G

T

A

Q

D

I

V

E

R

M

L

C

V

D

-

A

-

L

-

T

-

D

-

P

P

N

D

A

R

Q

S

L

W

E

E

E

D

L

A

A

L

T

E

R

S

V

V

E

R

A

A

Q

A

G

G

F

G

R

K

P

P

Y

Y

V

A

I

I

P

P

V
x
A

G
|
G

G

C

S
|
S

N

D

-

H

A

P

L

L

G

G

A

G

L

L

G

G

Y

F

V

V

E

G

S

F

A

A

L

E

E

E

I

V

-

R

A

A

Q

Q

Q

E

C

A

E

E

G

L

A

G

V

F

N

K

I

F

S

D

S

Y

V

V

A
x
C

S
|
S

G
x
V

S
x
T

A
x
G

G
x
S

T
|
T

H

Q

A

A

G

G

L

M

A

V

V

V

G

G

L

F

E

A

H

A

L

D

M

G

P

R

E

A

S

D

E

R

L

V

I

I

G

G

V

V

T

D

V

A

S

S

R

A

S

K

V

P

A

A

D

Q

Q

T

L

R

P

E

K

Q

V

I

V

T

N

R

L

I

Q

A

Q

R

A

Q

I

T

A

A

K

E

E

K

L

V

E

G

L

L

T

E

A

R

S

D

-

I

-

M

-

R

A

A

E

D

I

V

L

V

L

L

W

D

D

E

D

R

Y

F

F

A

A

G

P

P

G

E

Y
|
Y

G

G

V

L

P

P

N

N

D

E

E

G

G

T

M

L

E

E

A

A

V

I

K

R

L

L

L

C

A

A

R

R

L

T

E

E

G

G

I

M

L

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

A

H

G

G

L

M

I

I

D

E

G

M

I

V

S

R

Q

N

K

G

R

E

F

F

K

P

D

E

E

G

G

S

P

R

I

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

A

V

P

P

A

A

L

L

F

N

A

G

Y

Y

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding 3-chloro-D-alanine: K51 (= K51), I73 (≠ A73), G74 (= G74), S78 (= S78), N79 (= N79), Q80 (≠ H80), W102 (≠ P102), A153 (≠ V158), G154 (= G159), Y287 (= Y287)
binding amino-acrylate: K51 (= K51), S78 (= S78), N79 (= N79), Q80 (≠ H80), G154 (= G159), T192 (≠ S195), Y287 (= Y287)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), S156 (= S161), C189 (≠ A192), S190 (= S193), V191 (≠ G194), T192 (≠ S195), G193 (≠ A196), S194 (≠ G197), T195 (= T198), Y287 (= Y287), E288 (≠ T288), L315 (≠ T315), G316 (= G316), G317 (= G317)

1tzkA Crystal structure of 1-aminocyclopropane-1-carboxylate-deaminase complexed with alpha-keto-butyrate (see paper)
38% identity, 98% coverage: 5:324/328 of query aligns to 2:324/331 of 1tzkA

query
sites
1tzkA

N

N

L

L

T

Q

R

R

F

F

P

P

R

R

L

Y

E

P

F

L

I

T

G

F

A

G

P

P

T

T

P

P

L

I

E

Q

Y

P

L

L

P

A

R

R

F

L

S

S

D

K

Y

H

L

L

G

G

R

G

E

K

I

V

-

H

-

L

F

Y

I

A

K

K

R

R

D

E

D

D

V

C

T

N

P

S

-

G

M

L

A

A

M

F

G

G

N
|
N

K
|
K

L

T

R

R

K
|
K

L

L

E

E

F

Y

L

L

A

I

A

P

D

E

A

A

L

L

R

A

E

Q

G

G

A

C

D

D

T

T

L

L

I

V

T

S

A
x
I

G
|
G

A

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

V

T

R

R

Q

Q

T

V

A

A

V

V

A

A

K

H

L

L

G

G

L

M

H

K

C

C

V

V

A

L

L

V

L

Q

E

E

N

N

P

W

I

V

G

N

T

Y

T

S

A

D

E

A

N

V

Y

Y

L

D

T

R

N

V

G

G

N

N

R

I

L

Q

L

M

L

S

D

R

L

I

F

L

N

G

T

A

Q

D

I

V

E

R

M

L

C

V

D

-

A

-

L

-

T

-

D

-

P

P

N

D

A

R

Q

S

L

W

E

E

E

D

L

A

A

L

T

E

R

S

V

V

E

R

A

A

Q

A

G

G

F

G

R

K

P

P

Y

Y

V

A

I

I

P

P

V
x
A

G
|
G

G

C

S
|
S

N

D

-

H

A

P

L

L

G

G

A

G

L

L

G

G

Y

F

V

V

E

G

S

F

A

A

L

E

E

E

I

V

-

R

A

A

Q

Q

Q

E

C

A

E

E

G

L

A

G

V

F

N

K

I

F

S

D

S

Y

V

V

A
x
C

S
|
S

G
x
V

S
x
T

A
x
G

G
x
S

T
|
T

H

Q

A

A

G

G

L

M

A

V

V

V

G

G

L

F

E

A

H

A

L

D

M

G

P

R

E

A

S

D

E

R

L

V

I

I

G

G

V

V

T

D

V

A

S

S

R

A

S

K

V

P

A

A

D

Q

Q

T

L

R

P

E

K

Q

V

I

V

T

N

R

L

I

Q

A

Q

R

A

Q

I

T

A

A

K

E

E

K

L

V

E

G

L

L

T

E

A

R

S

D

-

I

-

M

-

R

A

A

E

D

I

V

L

V

L

L

W

D

D

E

D

R

Y

F

F

A

A

G

P

P

G

E

Y
|
Y

G

G

V

L

P

P

N

N

D

E

E

G

G

T

M

L

E

E

A

A

V

I

K

R

L

L

L

C

A

A

R

R

L

T

E

E

G

G

I

M

L

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

A

H

G

G

L

M

I

I

D

E

G

M

I

V

S

R

Q

N

K

G

R

E

F

F

K

P

D

E

E

G

G

S

P

R

I

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

A

V

P

P

A

A

L

L

F

N

A

G

Y

Y

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding 2-ketobutyric acid: K51 (= K51), I73 (≠ A73), G74 (= G74), S78 (= S78), N79 (= N79), Q80 (≠ H80), A153 (≠ V158), G154 (= G159), Y287 (= Y287)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), S156 (= S161), C189 (≠ A192), S190 (= S193), V191 (≠ G194), T192 (≠ S195), G193 (≠ A196), S194 (≠ G197), T195 (= T198), Y287 (= Y287), E288 (≠ T288), L315 (≠ T315), G316 (= G316), G317 (= G317)

1tzjA Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase complexed with d-vinyl glycine (see paper)
38% identity, 98% coverage: 5:324/328 of query aligns to 2:324/331 of 1tzjA

query
sites
1tzjA

N

N

L

L

T

Q

R

R

F

F

P

P

R

R

L

Y

E

P

F

L

I

T

G

F

A

G

P

P

T

T

P

P

L

I

E

Q

Y

P

L

L

P

A

R

R

F

L

S

S

D

K

Y

H

L

L

G

G

R

G

E

K

I

V

-

H

-

L

F

Y

I

A

K

K

R

R

D

E

D

D

V

C

T

N

P

S

-

G

M

L

A

A

M

F

G

G

N
|
N

K
|
K

L

T

R

R

K
|
K

L

L

E

E

F

Y

L

L

A

I

A

P

D

E

A

A

L

L

R

A

E

Q

G

G

A

C

D

D

T

T

L

L

I

V

T

S

A
x
I

G
|
G

A

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

V

T

R

R

Q

Q

T

V

A

A

V

V

A

A

K

H

L

L

G

G

L

M

H

K

C

C

V

V

A

L

L

V

L

Q

E

E

N

N

P

W

I

V

G

N

T

Y

T

S

A

D

E

A

N

V

Y

Y

L

D

T

R

N

V

G

G

N

N

R

I

L

Q

L

M

L

S

D

R

L

I

F

L

N

G

T

A

Q

D

I

V

E

R

M

L

C

V

D

-

A

-

L

-

T

-

D

-

P

P

N

D

A

R

Q

S

L

W

E

E

E

D

L

A

A

L

T

E

R

S

V

V

E

R

A

A

Q

A

G

G

F

G

R

K

P

P

Y

Y

V

A

I

I

P

P

V
x
A

G
|
G

G

C

S
|
S

N

D

-

H

A

P

L

L

G

G

A

G

L

L

G

G

Y

F

V

V

E

G

S

F

A

A

L

E

E

E

I

V

-

R

A

A

Q

Q

Q

E

C

A

E

E

G

L

A

G

V

F

N

K

I

F

S

D

S

Y

V

V

A
x
C

S
|
S

G
x
V

S
x
T

A
x
G

G
x
S

T
|
T

H

Q

A

A

G

G

L

M

A

V

V

V

G

G

L

F

E

A

H

A

L

D

M

G

P

R

E

A

S

D

E

R

L

V

I

I

G

G

V

V

T

D

V

A

S

S

R

A

S

K

V

P

A

A

D

Q

Q

T

L

R

P

E

K

Q

V

I

V

T

N

R

L

I

Q

A

Q

R

A

Q

I

T

A

A

K

E

E

K

L

V

E

G

L

L

T

E

A

R

S

D

-

I

-

M

-

R

A

A

E

D

I

V

L

V

L

L

W

D

D

E

D

R

Y

F

F

A

A

G

P

P

G

E

Y
|
Y

G

G

V

L

P

P

N

N

D

E

E

G

G

T

M

L

E

E

A

A

V

I

K

R

L

L

L

C

A

A

R

R

L

T

E

E

G

G

I

M

L

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

A

H

G

G

L

M

I

I

D

E

G

M

I

V

S

R

Q

N

K

G

R

E

F

F

K

P

D

E

E

G

G

S

P

R

I

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

A

V

P

P

A

A

L

L

F

N

A

G

Y

Y

active site: K51 (= K51), Y261 (= Y261), Y287 (= Y287)
binding d-vinylglycine: K51 (= K51), I73 (≠ A73), G74 (= G74), S78 (= S78), N79 (= N79), Q80 (≠ H80), A153 (≠ V158), G154 (= G159), Y287 (= Y287)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54)