SitesBLAST
Comparing 16506 FitnessBrowser__Keio:16506 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P45563 Purine nucleoside phosphorylase 2; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase II; PNP II; Xanthosine phosphorylase; EC 2.4.2.1 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:277/277 of query aligns to 1:277/277 of P45563
- H65 (= H65) binding
- RGH 85:87 (= RGH 85:87) binding
- A117 (= A117) binding
- Y191 (= Y191) mutation to L: No detectable activity with xanthosine as substrate, but largely retains its activity against other substrates, namely inosine and guanosine, although with altered affinities, higher and lower respectively, and clearly reduced maximal velocities for both.
- E197 (= E197) binding
- S216 (= S216) binding
- N239 (= N239) binding ; mutation to D: Catalyzes the phosphorolysis of adenosine with moderate efficiency, and essentially has lost all activity against the 6-oxo-purine substrates xanthosine, inosine and guanosine.
1yr3A Escherichia coli purine nucleoside phosphorylase ii, the product of the xapa gene (see paper)
100% identity, 99% coverage: 5:277/277 of query aligns to 1:273/273 of 1yr3A
- active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
- binding xanthine: A114 (= A118), E193 (= E197), G210 (= G214), N235 (= N239)
1yqqA Escherichia coli purine nucleoside phosphorylase ii, the product of the xapa gene (see paper)
100% identity, 99% coverage: 5:277/277 of query aligns to 1:273/273 of 1yqqA
- active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
- binding guanine: A114 (= A118), G115 (= G119), E193 (= E197), G210 (= G214), M211 (= M215), T234 (= T238), N235 (= N239), T250 (= T254)
- binding phosphate ion: H61 (= H65), R81 (= R85), H83 (= H87), N112 (= N116), S212 (= S216)
3odgA Crystal structure of xanthosine phosphorylase bound with xanthine from yersinia pseudotuberculosis
70% identity, 99% coverage: 5:277/277 of query aligns to 1:273/273 of 3odgA
- active site: S30 (= S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), N235 (= N239), A237 (= A241), H247 (= H251)
- binding xanthine: A114 (= A118), G115 (= G119), F192 (= F196), E193 (= E197), G210 (= G214), M211 (= M215), T234 (= T238), N235 (= N239)
P46354 Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 from Bacillus subtilis (strain 168) (see paper)
47% identity, 91% coverage: 17:269/277 of query aligns to 12:263/271 of P46354
- S28 (= S34) modified: Phosphoserine
8swuA Structure of clostridium perfringens pnp bound to transition state analog immucillin h and sulfate (see paper)
44% identity, 92% coverage: 21:276/277 of query aligns to 18:269/269 of 8swuA
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: Y82 (= Y89), A110 (= A117), A111 (= A118), G112 (= G119), Y189 (≠ F196), E190 (= E197), M208 (= M215), N232 (= N239), H244 (= H251), V247 (≠ T254)
4m1eB Crystal structure of purine nucleoside phosphorylase i from planctomyces limnophilus dsm 3776, nysgrc target 029364.
43% identity, 91% coverage: 26:277/277 of query aligns to 22:273/273 of 4m1eB
- active site: T30 (≠ S34), H61 (= H65), H83 (= H87), Y85 (= Y89), E86 (= E90), A113 (= A117), M211 (= M215), S212 (= S216), D235 (≠ N239), C237 (vs. gap), L247 (= L249)
- binding pyridine-2-carboxylic acid: N118 (≠ R122), Q120 (≠ E124), R169 (≠ Q173), H178 (≠ P182), P180 (≠ T184), P180 (≠ T184)
- binding adenine: A114 (= A118), G115 (= G119), E193 (= E197), V209 (= V213), D235 (≠ N239)
A4Q998 Purine nucleoside phosphorylase; AgPNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Anopheles gambiae (African malaria mosquito) (see paper)
44% identity, 86% coverage: 3:240/277 of query aligns to 67:309/353 of A4Q998
- S98 (= S34) binding
- H129 (= H65) binding
- RFH 149:151 (≠ RGH 85:87) binding
- A181 (= A117) binding
- S285 (= S216) binding
Sites not aligning to the query:
- 1:68 modified: Variant sequence, MSKFSYLQNGKASTNGVPHANGHHQQHQNGHSNGVARNGGTATDTLPVAYQQKAATSGPFHMPRTEHV -> M (in isoform 2)
2p4sA Structure of purine nucleoside phosphorylase from anopheles gambiae in complex with dadme-immh (see paper)
46% identity, 81% coverage: 17:241/277 of query aligns to 9:241/282 of 2p4sA
- active site: S29 (= S34), H60 (= H65), H82 (= H87), Y84 (= Y89), E85 (= E90), A112 (= A117), M215 (= M215), S216 (= S216), N239 (= N239), C241 (≠ A241)
- binding 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one: Y84 (= Y89), A112 (= A117), A113 (= A118), G114 (= G119), F196 (= F196), E197 (= E197), I213 (≠ V213), G214 (= G214), M215 (= M215), N239 (= N239)
- binding phosphate ion: G28 (= G33), S29 (= S34), R80 (= R85), H82 (= H87), N111 (= N116), S216 (= S216)
Sites not aligning to the query:
3phbE Crystal structure of human purine nucleoside phosphorylase in complex with dadme-immg
44% identity, 91% coverage: 26:276/277 of query aligns to 28:285/288 of 3phbE
- active site: S36 (= S34), H67 (= H65), H89 (= H87), Y91 (= Y89), E92 (= E90), A119 (= A117), M222 (= M215), S223 (= S216), N246 (= N239), V248 (vs. gap), H260 (= H251)
- binding 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one: Y91 (= Y89), A119 (= A117), A120 (= A118), G121 (= G119), F203 (= F196), E204 (= E197), V220 (= V213), G221 (= G214), M222 (= M215), N246 (= N239), H260 (= H251)
3inyA Crystal structure of human purine nucleoside phosphorylase in complex with 7-deazaguanine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 3inyA
- active site: S32 (= S34), H63 (= H65), H85 (= H87), Y87 (= Y89), E88 (= E90), A115 (= A117), M218 (= M215), S219 (= S216), N242 (= N239), V244 (vs. gap), H256 (= H251)
- binding 7-deazaguanine: A115 (= A117), A116 (= A118), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), T241 (= T238), N242 (= N239)
3d1vA Crystal structure of human pnp complexed with 2-mercapto(3h) quinazolinone (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 3d1vA
- active site: S32 (= S34), H63 (= H65), H85 (= H87), Y87 (= Y89), E88 (= E90), A115 (= A117), M218 (= M215), S219 (= S216), N242 (= N239), V244 (vs. gap), H256 (= H251)
- binding 2-mercapto(3H)quinazolinone: A115 (= A117), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), T241 (= T238), N242 (= N239)
1yryE Crystal structure of human pnp complexed with mesg (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1yryE
- active site: S32 (= S34), H63 (= H65), H85 (= H87), Y87 (= Y89), E88 (= E90), A115 (= A117), M218 (= M215), S219 (= S216), N242 (= N239), H256 (= H251)
- binding 7-methyl-6-thio-guanosine: Y87 (= Y89), A115 (= A117), A116 (= A118), G117 (= G119), E200 (= E197), V216 (= V213), G217 (= G214), M218 (= M215), T241 (= T238), N242 (= N239), H256 (= H251)
1v45E Crystal structure of human pnp complexed with 3-deoxyguanosine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1v45E
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding 9-(3-deoxy-beta-d-ribofuranosyl)guanine: A115 (= A117), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), N242 (= N239), H256 (= H251)
1v41E Crystal structure of human pnp complexed with 8-azaguanine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1v41E
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding 5-amino-1h-[1,2,3]triazolo[4,5-d]pyrimidin-7-ol: A115 (= A117), A116 (= A118), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), T241 (= T238), N242 (= N239)
1v3qE Structure of human pnp complexed with ddi (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1v3qE
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding 9-[(2r,5r)-5-(hydroxymethyl)tetrahydrofuran-2-yl]-1,9-dihydro-6h-purin-6-one: G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), N242 (= N239), H256 (= H251)
1v2hE Crystal structure of human pnp complexed with guanine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1v2hE
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding guanine: A116 (= A118), G117 (= G119), E200 (= E197), M218 (= M215), T241 (= T238), N242 (= N239)
1rfgE Crystal structure of human purine nucleoside phosphorylase complexed with guanosine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1rfgE
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding guanosine: A115 (= A117), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), T241 (= T238), N242 (= N239), H256 (= H251)
1rctE Crystal structure of human purine nucleoside phosphorylase complexed with inosine (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1rctE
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding inosine: A115 (= A117), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), N242 (= N239), H256 (= H251)
1pwyE Crystal structure of human pnp complexed with acyclovir (see paper)
44% identity, 91% coverage: 26:276/277 of query aligns to 24:281/288 of 1pwyE
- active site: S32 (= S34), H63 (= H65), Y87 (= Y89), A115 (= A117), M218 (= M215), S219 (= S216), H256 (= H251)
- binding 9-hyroxyethoxymethylguanine: A115 (= A117), A116 (= A118), G117 (= G119), F199 (= F196), E200 (= E197), V216 (= V213), M218 (= M215), T241 (= T238), N242 (= N239), H256 (= H251)
Query Sequence
>16506 FitnessBrowser__Keio:16506
MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIENAVAISYEKLPGFPVS
TVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGS
LRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKEEG
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITNM
AEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKIA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory