SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16795 FitnessBrowser__Keio:16795 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTK9 Rubredoxin-NAD(+) reductase; RdxR; EC 1.18.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
34% identity, 99% coverage: 5:376/377 of query aligns to 7:380/384 of Q9HTK9

query
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Q9HTK9

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TGLA 12:15 (≠ SGFA 10:13) binding
TA 36:37 (≠ AA 34:35) binding
K45 (= K43) binding
V83 (= V81) binding
E159 (= E155) binding
D277 (= D273) binding
V289 (≠ L285) binding
K320 (= K316) binding

2v3aA Crystal structure of rubredoxin reductase from pseudomonas aeruginosa. (see paper)
34% identity, 99% coverage: 5:376/377 of query aligns to 4:377/381 of 2v3aA

query
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active site: L11 (≠ F12), K42 (= K43), P43 (= P44), M290 (≠ Q289)
binding flavin-adenine dinucleotide: I7 (= I8), G8 (= G9), T9 (≠ S10), G10 (= G11), A12 (= A13), T33 (≠ A34), A34 (= A35), D35 (= D36), K42 (= K43), P43 (= P44), R79 (≠ W80), V80 (= V81), A105 (= A106), W106 (≠ T107), G107 (= G108), I153 (= I152), F157 (≠ L156), D274 (= D273), L284 (≠ P283), Y285 (≠ F284), V286 (≠ L285), K317 (= K316)

1xhcA Nadh oxidase /nitrite reductase from pyrococcus furiosus pfu-1140779- 001
31% identity, 88% coverage: 5:335/377 of query aligns to 3:321/346 of 1xhcA

query
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1xhcA

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active site: P10 (≠ F12), K38 (= K43), P39 (= P44), F145 (≠ L151), E149 (= E155), M276 (≠ Q289)
binding flavin-adenine dinucleotide: V6 (≠ I8), G7 (= G9), G9 (= G11), P10 (≠ F12), G11 (≠ A13), D29 (≠ A34), K30 (≠ A35), K38 (= K43), P39 (= P44), E74 (≠ Q78), A75 (≠ T79), A100 (= A106), T101 (= T107), G102 (= G108), L119 (= L125), R120 (≠ N126), F145 (≠ L151), I146 (= I152), E149 (= E155), G259 (= G272), D260 (= D273), G270 (≠ P283), T271 (≠ F284), A272 (≠ L285)

Q8U1K9 NAD(P)H:rubredoxin oxidoreductase; NROR; Rubredoxin--NAD(P)(+) reductase; EC 1.18.1.4 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
31% identity, 88% coverage: 5:335/377 of query aligns to 3:321/359 of Q8U1K9

query
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Q8U1K9

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R

K

V

V

K

I

S

T

Q

E

N

K

N

G

Q

E

W

V

Q

P

Y

Y

D

D

K

T

L

L

V

V

L

L

A

A

T

T

G

G

A
|
A

S

R

A

A

F

R

V

E

P

P

P

Q

V

I

P

K

G

G

R

K

E

E

L

Y

M

L

L

L

T

T

L

L

N
x
R

S

T

Q

I

Q

F

E

D

Y

A

R

D

A

R

C

I

E

K

T

E

Q

S

L

I

R

E

D

N

A

S

R

G

R

E

V

A

L

I

I

I

V

I

G

G

L

F

I

I

G

G

S

L

E

E

L

L

A

A

M

G

D

N

F

L

C

A

R

E

A

A

G

G

K

Y

A

H

V

V

T

K

L

L

I

I

D

H

N

R

A

G

A

A

S

M

I

F

L

L

A

G

S

-

L

-

M

L

P

D

P

E

E

E

V

L

S

S

S

N

R

M

L

I

Q

K

H

D

R

M

L

L

T

E

E

E

M

T

G

G

V

V

H

K

L

F

L

L

K

N

S

S

Q

E

L

L

Q

-

G

-

L

L

E

E

K

A

T

N

D

E

S

E

G

G

I

V

Q

-

A

-

T

-

L

L

D

T

R

N

Q

S

R

G

N

F

I

I

E

E

V

G

D

K

A

V

V

K

I

I

A

C

A

A

T

I

G

G

L

I

R

V

P

P

E

N

T

V

A

D

L

L

A

A

R

R

A

S

G

G

L

I

T

H

I

T

N

G

R

R

G

G

V

I

C

L

V

I

D

D

S

D

Y

N

L

F

Q

R

T

T

S

S

N

A

T

K

D

D

I

V

Y

Y

A

A

L

I

G

G

D
|
D

C

C

A

A

E

E

I

Y

N

S

G

G

Q

I

V

I

L

A

P

G

F

T

L

A

Q

K

P

A

I

A

Q

M

L

E

S

Q

A

A

M

R

V

V

L

L

A

A

K

D

N

I

L

L

L

K

G

G

N

E

N

P

T

R

P

R

L

Y

K

N

L

F

P

K

A

F

M

R

L

S

V

T

K

V

I

F

K

K

T

F

P

G

E

K

L

L

P

Q

L

I

H

A

L

I

A

I

G

G

E

N

T

T

Q

K

R

G

Q

E

D

G

L

-

R

K

W

W

G11 (≠ A13) binding
DK 29:30 (≠ AA 34:35) binding
KP 38:39 (= KP 43:44) binding
A75 (vs. gap) binding
A103 (= A109) binding
R120 (≠ N126) binding
D260 (= D273) binding

1q1wA Crystal structure of putidaredoxin reductase from pseudomonas putida (see paper)
30% identity, 74% coverage: 2:281/377 of query aligns to 3:291/422 of 1q1wA

query
sites
1q1wA

S

N

N

D

G

N

I

V

V

V

I

I

I

V

G
|
G

S

T

G
|
G

F
x
L

A
|
A

A

G

R

V

Q

E

L

V

V

A

K

F

N

G

I

L

R

R

K

A

Q

S

D

G

A

W

T

E

I

G

P

N

L

I

T

R

L

L

I

V

A
x
G

A
x
D

D

A

S

T

M

V

D

I

E

P

Y

H

N

H

K
x
L

P
|
P

D

P

L

L

S

S

H
x
K

V

A

I

Y

S

L

Q

A

G

G

Q

K

-

A

R

T

A

A

D

E

D

S

L

L

T

Y

R

L

Q

R

T

T

A

P

G

D

E

A

F

Y

A

A

E

A

Q

Q

F

-

N

N

L

I

H

Q

L

L

F

L

P

G

Q

G

T

T

W

Q

V
|
V

T

T

D

A

I

I

D

N

A

R

E

D

-

R

A

Q

R

Q

V

V

V

I

K

L

S

S

Q

D

N

G

N

R

Q

A

W

L

Q

D

Y

Y

D

D

K

R

L

L

V

V

L

L

A
|
A

T
|
T

G
|
G

A

G

S

R

A

P

F

R

V

P

P

L

P

P

V

V

P

A

G

S

R

G

E

A

L

V

-

G

-

K

-

A

-

N

M

F

L

R

T

Y

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

E

A

C

C

I

E

R

T

R

Q

Q

L

L

R

I

D

A

A

D

R

N

R

R

V

L

L

V

I

V

V

I

G

G

L

Y

I
|
I

G

G

S

L

E

E

L

V

A

A

M

A

D

T

F

A

C

I

R

K

A

A

G

N

K

M

A

H

V

V

T

T

L

L

I

L

D

D

N

T

A

A

S

R

I

V

L

L

A

E

S

R

L

V

M

T

P

A

P

P

E

P

V

V

S

S

S

A

R

F

L

Y

Q

E

H

H

R

L

L

H

T

R

E

E

M

A

G

G

V

V

H

D

L

I

L

R

L

T

K

G

S

T

Q

Q

L

V

Q

C

G

G

L

F

E

E

K

M

T

S

D

T

S

D

G

Q

I

Q

Q

K

A

V

T

T

-

A

-

V

-

L

L

C

D

E

R

D

Q

G

R

T

N

R

I

L

E

P

V

A

D

D

A

L

V

V

I

I

A

A

A

G

T

I

G

G

L

L

R

I

P

P

E

N

T

C

A

E

L

L

A

A

R

S

R

A

A

A

G

G

L

L

T

Q

I

V

N

D

R

N

G

G

V

I

C

V

V

I

D

N

S

E

Y

H

L

M

Q

Q

T

T

S

S

N

D

T

P

D

L

I

I

Y

M

A

A

L

V

G

G

D
|
D

C

C

A

A

E

R

I

F

N

H

G

S

Q

Q

V

L

active site: L13 (≠ F12), L44 (≠ K43), P45 (= P44)
binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), L13 (≠ F12), A14 (= A13), G35 (≠ A34), D36 (≠ A35), L44 (≠ K43), P45 (= P44), K49 (≠ H48), V82 (= V81), A108 (= A106), T109 (= T107), G110 (= G108), R133 (≠ N126), I159 (= I152), D283 (= D273)

Sites not aligning to the query:

active site: 305
binding flavin-adenine dinucleotide: 300, 301, 329

P16640 Putidaredoxin reductase CamA; Pdr; Putidaredoxin--NAD(+) reductase; EC 1.18.1.5 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
30% identity, 74% coverage: 2:281/377 of query aligns to 4:292/422 of P16640

query
sites
P16640

S

N

N

D

G

N

I

V

V

V

I

I

I

V

G

G

S

T

G

G

F

L

A
|
A

A

G

R

V

Q

E

L

V

V

A

K

F

N

G

I

L

R

R

K

A

Q

S

D

G

A

W

T

E

I

G

P

N

L

I

T

R

L

L

I

V

A

G

A
x
D

D

A

S

T

M

V

D

I

E

P

Y

H

N

H

K

L

P

P

D

P

L

L

S

S

H
x
K

V

A

I

Y

S

L

Q

A

G

G

Q

K

-

A

R

T

A

A

D

E

D

S

L

L

T

Y

R

L

Q

R

T

T

A

P

G

D

E

A

F

Y

A

A

E

A

Q

Q

F

-

N

N

L

I

H

Q

L

L

F

L

P

G

Q

G

T

T

W

Q

V
|
V

T

T

D

A

I

I

D

N

A

R

E

D

-

R

A

Q

R

Q

V

V

V

I

K

L

S

S

Q

D

N

G

N

R

Q

A

W

L

Q

D

Y

Y

D

D

K

R

L

L

V

V

L

L

A

A

T

T

G

G

A

G

S

R

A

P

F

R

V

P

P

L

P

P

V

V

P

A

G

S

R

G

E

A

L

V

-

G

-

K

-

A

-

N

M

F

L

R

T

Y

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

E

A

C

C

I

E

R

T

R

Q

Q

L

L

R

I

D

A

A

D

R

N

R

R

V

L

L

V

I

V

V

I

G

G

L

Y

I

I

G

G

S

L

E

E

L

V

A

A

M

A

D

T

F

A

C

I

R

K

A

A

G

N

K

M

A

H

V

V

T

T

L

L

I

L

D

D

N

T

A

A

S

R

I

V

L

L

A

E

S

R

L

V

M

T

P

A

P

P

E

P

V

V

S

S

S

A

R

F

L

Y

Q

E

H

H

R

L

L

H

T

R

E

E

M

A

G

G

V

V

H

D

L

I

L

R

L

T

K

G

S

T

Q

Q

L

V

Q

C

G

G

L

F

E

E

K

M

T

S

D

T

S

D

G

Q

I

Q

Q

K

A

V

T

T

-

A

-

V

-

L

L

C

D

E

R

D

Q

G

R

T

N

R

I

L

E

P

V

A

D

D

A

L

V

V

I

I

A

A

A

G

T

I

G

G

L

L

R

I

P

P

E

N

T

C

A

E

L

L

A

A

R

S

R

A

A

A

G

G

L

L

T

Q

I

V

N

D

R

N

G

G

V

I

C

V

V

I

D

N

S

E

Y

H

L

M

Q

Q

T

T

S

S

N

D

T

P

D

L

I

I

Y

M

A

A

L

V

G

G

D
|
D

C

C

A

A

E

R

I

F

N

H

G

S

Q

Q

V

L

A15 (= A13) binding
D37 (≠ A35) binding
K50 (≠ H48) binding
V83 (= V81) binding
R134 (≠ N126) binding
D284 (= D273) binding

Sites not aligning to the query:

302 binding

6pfzA Structure of a NAD-dependent persulfide reductase from a. Fulgidus (see paper)
27% identity, 79% coverage: 5:302/377 of query aligns to 3:322/541 of 6pfzA

query
sites
6pfzA

I

V

V

V

I

V

I

I

G
|
G

S

G

G

G

F

A

A
|
A

A

G

R

L

Q
x
K

L

A

V

A

K

S

N
x
R

I

I

R

R

K
x
R

Q

K

D

D

A

G

T

D

I

A

P

S

L

I

T

T

L

V

I

V

A
x
E

A
|
A

D

G

S

K

M

Y

D
x
V

E
x
S

Y

L

N
x
G

K
x
R

P
x
C

D

G

L

L

S

P

H

Y

V

Y

I

V

S

G

Q

G

-

L

G

V

Q

H

R

E

A

V

D

D

D

N

L

L

T

R

R

E

Q

T

T

T

A

Y

G

G

E

A

-

V

-

R

-

D

-

E

-

A

-

Y

F

F

A

K

E

K

Q

L

F
x
K

N

N

L

I

H

D

L

V

F

L

P

T

Q

E

T

T

W

V

V
x
A

T

T

D

E

I

I

D

D

A

R

E

S

A

R

R

K

V

T

V

V

K

K

-

I

-

V

-

R

-

N

S

G

Q

S

N

E

N

D

Q

E

W

L

Q

N

Y

Y

D

D

K

Y

L

L

V

V

L

I

A
|
A

T
|
T

G
|
G

A

A

S

R

A

P

F

A

V

K

P

P

V

I

P

E

G

G

R

I

E

E

L

A

-

E

-

G

M

V

L

V

T

T

L
|
L

N

T

S

S

Q

A

Q

E

E

E

Y

A

-

E

R

K

A

I

C

I

E

E

T

M

Q

W

L

E

R

E

D

G

A

A

R

E

R

K

V

A

L

V

I

V

V

I

G

G

G

A

G

G

L

F

I

I

G

G

S

L

E

E

L

S

A

A

M

E

D

A

F

L

C

K

R

N

A

L

G

D

K

M

A

E

V

V

T

T

L

V

I

I

D

E

N

M

A

M

A

D

S

R

I

V

L

A

A

P

S

A

L

M

M

L

P

D

P

R

E

E

V

M

S

A

S

V

R

L

L

V

Q

E

H

N

R

H

L

L

T

R

E

E

M

K

G

G

V

V

H

N

L

V

L

T

K

S

S

T

Q

R

L

V

Q

E

G

K

L

I

E

V

K

S

T

Q

D

D

S

D

G

K

I

V

Q

R

A

A

T

V

L

I

D

A

R

N

Q

G

R

K

N

E

I

Y

E

P

V

A

D

D

A

V

V

V

I

V

A

V

A

A

T

T

G

G

L

I

R

K

P

P

E

N

T

S

A

E

L

L

A

A

R

E

R

K

A

A

G

G

L

L

T

K

I

I

N

G

R

E

-

T

-

G

G

A

V

I

C

W

V

V

D

D

S

E

Y

Y

L

M

Q

R

T

T

S

S

N

D

T

E

D

S

I

I

Y

Y

A

A

L

G

G
|
G

D
|
D

C

C

A

V

E

E

-

T

-

C

-

L

I

V

N

T

G

G

Q

K

-

K

-

I

V

I

L

A

P
|
P

F
|
F

L
x
G

Q
x
D

P

V

I

A

Q

N

L

K

S

Q

A

G

M
x
R

V

V

L

I

A

G

K

E

N

N

L

I

L

T

G

G

N

G

active site: V37 (≠ D39), C42 (≠ P44)
binding calcium ion: D306 (≠ Q286)
binding coenzyme a: K14 (≠ Q16), R18 (≠ N20), R21 (≠ K23), S38 (≠ E40), G40 (≠ N42), C42 (≠ P44), K76 (≠ F71), R313 (≠ M293)
binding flavin-adenine dinucleotide: G7 (= G9), A11 (= A13), E32 (≠ A34), A33 (= A35), R41 (≠ K43), C42 (≠ P44), A86 (≠ V81), A115 (= A106), T116 (= T107), G117 (= G108), L136 (= L125), G286 (= G272), D287 (= D273), P303 (= P283), F304 (= F284), G305 (≠ L285)

Sites not aligning to the query:

active site: 455, 456
binding calcium ion: 332, 427, 428
binding coenzyme a: 430, 447, 512, 513, 516

6tukB Crystal structure of fdr9 (see paper)
30% identity, 72% coverage: 5:275/377 of query aligns to 4:262/393 of 6tukB

query
sites
6tukB

I

I

V

V

I

I

I
x
V

G
|
G

S
x
G

G
|
G

F

L

A
|
A

A

A

R

S

Q

R

L

T

V

C

K

E

N

Q

I

L

R

R

K

S

Q

R

D

G

A

Y

T

E

I

G

P

E

L

L

T

V

L

M

I

L

A

C

A
|
A

D
x
E

S

P

M

H

D

P

E

P

Y

Y

N

D

K
x
R

P
|
P

D

P

L

L

S

S

H
x
K

V

A

I

A

S

L

Q

L

G

E

Q

E

R

E

A

H

D

D

D

S

L

T

T

F

R

P

Q

T

T

D

A

Y

G

A

E

Q

F

L

A

S

E

V

Q

D

F

V

N

R

L

L

H

G

L

V

F
x
A

P

-

Q

-

T

-

W

-

V
x
A

T

T

D

G

I

L

D

V

A

P

E

D

A

A

R

R

V

T

V

V

K

Q

S

T

Q

T

N

D

N

G

Q

E

W

L

Q

S

Y

Y

D

D

K

A

L

L

V

V

L

I

A

A

T
|
T

G
|
G

A

A

S

S

A

P

F

I

V

R

P

L

P

P

V

G

P

P

G

G

R

R

E

Q

L

F

M

T

L
x
V

-
x
R

T

T

L

V

N

E

S

D

Q

A

E

Q

Y

L

R

R

A

A

C

-

E

-

T

-

Q

E

L

L

R

K

D

P

A

G

R

Q

R

R

V

V

L

V

I

L

V

V

G

G

G

A

G

S

L

W

I

I

G

S

S

A

E

E

L

V

A

A

M

T

D

A

F

A

C

L

R

R

A

R

G

G

K

C

A

S

V

V

T

T

L

C

I

I

D

E

N

A

A

G

A

P

S

A

I

P

L

L

A

S

S

A

L

A

M

L

P

G

P

A

E

D

V

V

S

G

S

Q

R

R

L

F

Q

L

H

P

R

W

L

W

T

S

E

E

M

-

G

-

V

V

H

D

L

L

L

R

L

L

K

D

S

T

Q

G

L

V

Q

A

G

-

L

-

E

E

K

V

T

T

D

E

S

T

G

G

I

V

Q

Q

A

-

T

-

L

L

D

A

R

N

Q

G

R

E

N

Q

I

V

E

D

V

A

D

D

A

V

V

V

I

V

A

T

A

G

T

I

G

G

L

V

R

R

P

P

E

A

T

V

A

D

L

W

A

L

R

S

R

G

A

S

G

G

L

I

T

A

I

L

N

D

R

T

G

G

V

V

C

V

V

V

D

D

S

E

Y

H

L

L

Q

R

T

T

S

S

N

L

T

P

D

G

I

I

Y

Y

A

A

L

L

G
|
G

D
|
D

C

V

A

A

binding flavin-adenine dinucleotide: V7 (≠ I8), G8 (= G9), G9 (≠ S10), G10 (= G11), A12 (= A13), A34 (= A35), E35 (≠ D36), R42 (≠ K43), P43 (= P44), K47 (≠ H48), A75 (≠ F76), A76 (≠ V81), T102 (= T107), G103 (= G108), V118 (≠ L123), R119 (vs. gap), G259 (= G272), D260 (= D273)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 277, 278, 311, 312

4emjA Complex between the reductase and ferredoxin components of toluene dioxygenase (see paper)
28% identity, 73% coverage: 1:275/377 of query aligns to 1:277/406 of 4emjA

query
sites
4emjA

M

M

S

A

N

T

G

H

I

V

V

A

I

I

G

G

S

N

G
|
G

F
x
V

A
x
G

A

G

R

F

Q

T

L

T

V

A

K

Q

N

A

I

L

R

R

K

A

Q

E

D

G

A

F

T

E

I

G

P

R

L

I

T

S

L

L

I

I

A

G

A
x
D

D
x
E

S

P

M

H

D

L

E

P

Y

Y

N

D

K
x
R

P
|
P

D

S

L

L

S
|
S

H
x
K

V

A

I

V

S

L

Q

D

G

G

Q

S

R

L

A

E

D

R

D

P

L

P

T

I

R

L

Q

A

T

E

A

A

G

D

E

W

F

Y

A

G

E

E

-

A

Q

R

F

I

N

D

L

M

H

L

L

T

F

G

P

P

Q

E

T

-

W

-

V
|
V

T

T

D

A

I

L

D

D

A

V

E

Q

A

T

R

R

V

T

V

I

K

S

-

L

S

D

Q

D

N

G

N

T

Q

T

W

L

Q

S

Y

A

D

D

K

A

L

I

V

V

L

I

A

A

T
|
T

G
|
G

A

S

S

R

A

A

F

R

V

T

P

M

P

A

V

L

P

P

G

G

R

S

E

Q

L

L

-

P

-

G

M

V

L

V

T

T

L

L

N
x
R

S

T

Q

Y

Q

G

E

D

Y

V

R

Q

A

V

C

L

E

R

T

D

Q

S

L

W

R

T

D

S

A

A

R

T

R

R

V

L

L

L

I

I

V

V

G

G

L

L

I

I

G

G

S

C

E

E

L

V

A

A

M

T

D

T

F

A

C

R

R

K

A

L

G

G

K

L

A

S

V

V

T

T

L

I

I

L

D

E

N

A

A

G

A

D

S

E

I

L

L

L

A

V

S

R

L

V

M

L

P

G

P

R

E

R

V

I

S

G

S

A

R

W

L

L

Q

R

H

G

R

L

L

L

T

T

E

E

M

L

G

G

V

V

H

Q

L

V

L

E

L

L

K

G

S

T

Q

G

L

V

Q

V

G

G

L

F

E

S

K

G

T

E

D

G

S

Q

G

L

I

E

Q

Q

A

V

T

M

L

A

D

S

R

D

Q

G

R

R

N

S

I

F

E

V

V

A

D

D

A

S

V

A

I

L

A

I

A

C

T

V

G

G

L

A

R

E

P

P

E

A

T

D

A

Q

L

L

A

A

R

R

R

Q

A

A

G

G

L

L

T

A

I

C

N

D

R

R

G

G

V

V

C

I

V

V

D

D

S

H

Y

C

L

G

Q

A

T

T

S

L

N

A

T

K

D

G

I

V

Y

F

A

A

L

V

G
|
G

D
|
D

C

V

A

A

binding flavin-adenine dinucleotide: G11 (= G11), V12 (≠ F12), G13 (≠ A13), D35 (≠ A35), E36 (≠ D36), R43 (≠ K43), P44 (= P44), S47 (= S47), K48 (≠ H48), V80 (= V81), T107 (= T107), G108 (= G108), R128 (≠ N126), G274 (= G272), D275 (= D273)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 291, 292, 319, 320

4emiA Toluene dioxygenase reductase in reduced state in complex with NAD+ (see paper)
28% identity, 72% coverage: 5:275/377 of query aligns to 4:276/402 of 4emiA

query
sites
4emiA

I

V

V

A

I

I

G

G

S

N

G
|
G

F
x
V

A
x
G

A

G

R

F

Q

T

L

T

V

A

K

Q

N

A

I

L

R

R

K

A

Q

E

D

G

A

F

T

E

I

G

P

R

L

I

T

S

L

L

I

I

A

G

A
x
D

D
x
E

S

P

M

H

D

L

E

P

Y

Y

N

D

K
x
R

P
|
P

D

S

L

L

S

S

H
x
K

V

A

I

V

S

L

Q

D

G

G

Q

S

R

L

A

E

D

R

D

P

L

P

T

I

R

L

Q

A

T

E

A

A

G

D

E

W

F

Y

A

G

E

E

-

A

Q

R

F

I

N

D

L

M

H

L

L

T

F

G

P

P

Q
x
E

T

-

W

-

V
|
V

T

T

D

A

I

L

D

D

A

V

E

Q

A

T

R

R

V

T

V

I

K

S

-

L

S

D

Q

D

N

G

N

T

Q

T

W

L

Q

S

Y

A

D

D

K

A

L

I

V

V

L

I

A

A

T
|
T

G
|
G

A

S

S

R

A

A

F
x
R

V

T

P

M

P

A

V

L

P

P

G

G

R

S

E

Q

L

L

-

P

-

G

M

V

L

V

T

T

L

L

N

R

S

T

Q

Y

Q

G

E

D

Y

V

R

Q

A

V

C

L

E

R

T

D

Q

S

L

W

R

T

D

S

A

A

R

T

R

R

V

L

L

L

I

I

V

V

G
|
G

G

G

L
|
L

I
|
I

G

G

S

C

E
|
E

L

V

A

A

M

T

D

T

F

A

C

R

R

K

A

L

G

G

K

L

A

S

V

V

T

T

L

I

I

L

D
x
E

N
x
A

A

G

A

D

S

E

I

L

L

L

A

V

S
x
R

L

V

M

L

P

G

P

R

E

R

V

I

S

G

S

A

R

W

L

L

Q

R

H

G

R

L

L

L

T

T

E

E

M

L

G

G

V

V

H

Q

L

V

L

E

L

L

K

G

S

T

Q

G

L

V

Q

V

G

G

L

F

E

S

K

G

T

E

D

G

S

Q

G

L

I

E

Q

Q

A

V

T

M

L

A

D

S

R

D

Q

G

R

R

N

S

I

F

E

V

V

A

D

D

A

S

V

A

I

L

A

I

A

C

T
x
V

G
|
G

L
x
A

R

E

P

P

E

A

T

D

A

Q

L

L

A

A

R

R

R

Q

A

A

G

G

L

L

T

A

I

C

N

D

R

R

G

G

V

V

C

I

V

V

D

D

S

H

Y

C

L

G

Q

A

T

T

S

L

N

A

T

K

D

G

I

V

Y

F

A

A

L

V

G
|
G

D
|
D

C

V

A

A

binding flavin-adenine dinucleotide: G10 (= G11), V11 (≠ F12), G12 (≠ A13), D34 (≠ A35), E35 (≠ D36), R42 (≠ K43), P43 (= P44), K47 (≠ H48), E78 (≠ Q78), V79 (= V81), T106 (= T107), G107 (= G108), G273 (= G272), D274 (= D273)
binding nicotinamide-adenine-dinucleotide: R111 (≠ F112), G149 (= G148), L152 (= L151), I153 (= I152), E156 (= E155), E172 (≠ D171), A173 (≠ N172), R180 (≠ S179), V236 (≠ T235), G237 (= G236), A238 (≠ L237)

Sites not aligning to the query:

2cduA The crystal structure of water-forming NAD(p)h oxidase from lactobacillus sanfranciscensis (see paper)
31% identity, 53% coverage: 81:278/377 of query aligns to 81:287/451 of 2cduA

query
sites
2cduA

V
|
V

T

T

D

N

I

V

D

D

A

P

E

E

A

T

R

K

V

T

V

I

K

K

-

V

-

K

-

D

-

L

-

I

-

T

S

N

Q

E

N

E

N

K

Q

T

W

E

Q

A

Y

Y

D

D

K

K

L

L

V

I

L

M

A
x
T

T
|
T

G
|
G

A

S

S

K

A

P

F

T

V

V

P

P

V

I

P

P

G

G

-

I

-

D

-

S

R

S

E

R

L

V

M

Y

L

L

T

C

L
x
K

N

N

S

Y

Q

N

Q

D

E

A

Y

K

R

K

A

L

C

F

E

E

T

E

Q

A

L

P

R

K

D

-

A

A

R

K

R

T

V

I

L

T

I

I

V

I

G

G

G

S

G
|
G

L

Y

I
|
I

G

G

S

A

E

E

L

L

A

A

M

E

D

A

F

Y

C

S

R

N

A

Q

G

N

K

Y

A

N

V

V

T

N

L

L

I

I

D
|
D

N
x
G

A
x
H

A

E

S

R

I

V

L

L

A

Y

S

K

L
x
Y

M

F

P

D

P

K

E

E

V

F

S

T

S

D

R

I

L

L

Q

A

H

K

R

D

L

Y

T

E

E

A

M

H

G

G

V

V

H

N

L

L

L

V

L

L

K

G

S

S

Q
x
K

L
x
V

Q

A

G

A

L

F

E

E

K

E

T

V

D

D

S

D

G

E

-

I

I

I

Q

T

A

K

T

T

L

L

D

D

R

-

Q

G

R

K

N

E

I

I

E

K

V

S

D

D

A

I

V

A

I

I

A

L

A

C

T
x
I

G
|
G

L

F

R

R

P

P

E

N

T

T

A

E

L

L

A

L

R

K

-

G

R

K

A

V

G

A

L

M

T

L

I

D

N

N

R

G

G

A

V

I

C

I

V

T

D

D

S

E

Y

Y

L

M

Q

H

T

S

S

S

N

N

T

R

D

D

I

I

Y

F

A

A

L

A

G
|
G

D
|
D

C

S

A

A

E

A

I

V

N

H

binding adenosine-5'-diphosphate: G158 (= G150), I160 (= I152), D179 (= D171), G180 (≠ N172), H181 (≠ A173), Y188 (≠ L180), K213 (≠ Q205), V214 (≠ L206), I243 (≠ T235), G244 (= G236)
binding flavin-adenine dinucleotide: V81 (= V81), T112 (≠ A106), T113 (= T107), G114 (= G108), K134 (≠ L125), I160 (= I152), G281 (= G272), D282 (= D273)

Sites not aligning to the query:

active site: 10, 37, 41, 42, 304, 450, 451
binding flavin-adenine dinucleotide: 7, 9, 10, 11, 32, 33, 41, 42, 298, 299, 300

O84925 NADH oxidase; NOXase; EC 1.6.3.4 from Streptococcus pneumoniae (see paper)
31% identity, 54% coverage: 75:277/377 of query aligns to 75:298/459 of O84925

query
sites
O84925

L
x
V

F
x
Y

P
x
M

Q
x
N

T
x
S

W
x
P

V
|
V

T
x
L

D
x
S

I
|
I

D
|
D

A
x
Y

E
x
D

A
x
N

R
x
K

V
|
V

-
x
T

-
x
A

-
x
E

-
x
V

K
x
E

S
x
G

Q
x
K

N
x
E

N
x
H

Q
x
K

W
x
E

Q
x
S

Y
|
Y

D
x
E

K
|
K

L
|
L

V
x
I

L
x
F

A
|
A

T
|
T

G
|
G

A
x
S

S
x
T

A
x
P

F
x
I

V
x
L

P
|
P

V
x
I

P
x
E

G
|
G

-
x
V

-
x
E

-
x
I

-
x
V

-
x
K

-
x
G

-
x
N

R
|
R

E
|
E

L
x
F

M
x
K

L
x
A

T
|
T

L
|
L

N
x
E

S
x
N

Q
x
V

Q
|
Q

E
x
F

-
x
V

-
x
K

-
x
L

Y
|
Y

R
x
Q

A
x
N

C
x
A

E
|
E

-
x
E

-
x
V

-
x
I

T
x
N

Q
x
K

L
|
L

R
x
S

D
|
D

A
x
K

R
x
S

-
x
Q

-
x
H

-
x
L

-
x
D

R
|
R

V
x
I

L
x
A

I
x
V

V
|
V

G
|
G

L
x
Y

I
|
I

G
|
G

S
x
V

E
|
E

L
|
L

A
|
A

M
x
E

D
x
A

F
|
F

C
x
E

R
|
R

A
x
L

G
|
G

K
|
K

A
x
E

V
|
V

T
x
V

L
|
L

I
x
V

D
|
D

N
x
I

A
x
V

A
x
D

S
x
T

I
x
V

L
|
L

A
x
N

S
x
G

L
x
Y

M
x
Y

P
x
D

P
x
K

E
x
D

V
x
F

S
x
T

S
x
Q

R
x
M

L
x
M

Q
x
A

H
x
K

R
x
N

L
|
L

T
x
E

E
x
D

M
x
H

G
x
N

V
x
I

H
x
R

L
|
L

L
x
A

L
|
L

K
x
G

S
x
Q

Q
x
T

L
x
V

Q
x
K

G
x
A

L
x
I

E
|
E

K

-

T
x
G

D
|
D

S
x
G

G
x
K

I
x
V

Q
x
E

A
x
R

T
x
L

L
x
I

D
x
T

R
x
D

Q
x
K

R
x
E

N
x
S

I
x
F

E
x
D

V
|
V

D
|
D

A
x
M

V
|
V

I
|
I

A
x
L

A
|
A

T
x
V

G
|
G

L
x
F

R
|
R

P
|
P

E
x
N

T
|
T

A
|
A

L
|
L

A
|
A

R
x
D

-
x
G

R
x
K

A
x
I

G
x
E

L
|
L

T
x
F

I
x
R

N
|
N

R
x
G

G
x
A

V
x
F

C
x
L

V
|
V

D
|
D

S
x
K

Y
x
K

L
x
Q

Q
x
E

T
|
T

S
|
S

N
x
I

T
x
P

D
x
G

I
x
V

Y
|
Y

A
|
A

L
x
V

G
|
G

D
|
D

C
|
C

A
|
A

E
x
T

I
x
V

Sites not aligning to the query:

44 C→S: In Cp8054; abolishes NADH oxidase activity. Normal growth under aerobic conditions.
71:459 mutation Missing: In Cp8056; abolishes NADH oxidase activity. Normal growth under aerobic conditions.

2bcpA Structural analysis of streptococcus pyogenes nadh oxidase: c44s nox with azide (see paper)
30% identity, 55% coverage: 69:277/377 of query aligns to 86:313/473 of 2bcpA

query
sites
2bcpA

E

E

Q

S

F

L

N

G

L

A

H

K

L

V

F

Y

P

M

Q

E

T

S

W

P

V
|
V

T

Q

D

S

I

I

D

D

A

Y

E

D

A

A

R

K

V

T

V

V

K

T

S

A

-

L

-

V

-

D

-

G

Q

K

N

N

N

H

Q

V

W

E

Q

T

Y

Y

D

D

K

K

L

L

V

I

L

F

A
|
A

T
|
T

G
|
G

A

S

S

Q

A

P

F

I

V

L

P

P

V

I

P

K

G

G

R

A

E

E

L

I

M

K

-

E

-

G

-

S

-

L

-

E

-

F

-

E

L

A

T

T

L

L

N

E

S

N

Q

L

Q

Q

E

F

Y

V

R
x
K

A

L

C

Y

E

Q

T

N

-

S

-

A

-

D

-

V

-

I

-

A

-

K

-

L

Q

E

L

N

R

K

D

D

A

I

R

K

R

R

V

V

L

A

I

V

V

V

G

G

G

A

G

G

L
x
Y

I
|
I

G

G

S

V

E

E

L

L

A

A

M

E

D

A

F

F

C

Q

R

R

A

K

G

G

K

K

A

E

V

V

T

V

L

L

I

I

D

D

N

V

A

V

A

D

S

T

I

C

L

L

A

A

S

G

L

Y

M

Y

P

D

P

R

E

D

V

L

S

T

S

D

R

L

L

M

Q

A

H

K

R

N

L

M

T

E

E

E

M

H

G

G

V

I

H

Q

L

L

L

A

L

F

K

G

S

E

Q

T

L

V

Q

K

G

-

L

-

E

E

K

V

T

A

D

G

S

N

G

G

-

K

I

V

Q

E

A

K

T

I

L

I

D

T

R

D

Q

K

R

N

N

E

I

Y

E

D

V

V

D

D

A

M

V

V

I

I

A

L

A

A

T

V

G

G

L

F

R

R

P

P

E

N

T

T

A

T

L

L

A

G

R

N

-

G

R

K

A

I

G

D

L

L

T

F

I

R

N

N

R

G

G

A

V

F

C

L

V

V

D

N

S

K

Y

R

L

Q

Q

E

T

T

S

S

N

I

T

P

D

G

I

V

Y

Y

A

A

L

I

G
|
G

D
|
D

C

C

A

A

E

T

I

I

binding flavin-adenine dinucleotide: V98 (= V81), A127 (= A106), T128 (= T107), G129 (= G108), K160 (≠ R132), Y187 (≠ L151), I188 (= I152), G308 (= G272), D309 (= D273)

Sites not aligning to the query:

active site: 28, 56, 60, 61, 331
binding azide ion: 60, 61
binding flavin-adenine dinucleotide: 24, 25, 27, 28, 29, 51, 52, 60, 61, 326, 327

Q5XC60 NADH oxidase; NOXase; EC 1.6.3.4 from Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) (see paper)
30% identity, 55% coverage: 69:277/377 of query aligns to 69:296/456 of Q5XC60

query
sites
Q5XC60

E

E

Q

S

F

L

N

G

L

A

H

K

L

V

F

Y

P

M

Q

E

T

S

W

P

V
|
V

T

Q

D

S

I

I

D

D

A

Y

E

D

A

A

R

K

V

T

V

V

K

T

S

A

-

L

-

V

-

D

-

G

Q

K

N

N

N

H

Q

V

W

E

Q

T

Y

Y

D

D

K

K

L

L

V

I

L

F

A
|
A

T

T

G

G

A
x
S

S

Q

A

P

F

I

V

L

P

P

V

I

P

K

G

G

R

A

E

E

L

I

M

K

-

E

-

G

-

S

-

L

-

E

-

F

-

E

L

A

T

T

L

L

N

E

S

N

Q

L

Q

Q

E

F

Y

V

R
x
K

A

L

C

Y

E

Q

T

N

-

S

-

A

-

D

-

V

-

I

-

A

-

K

-

L

Q

E

L

N

R

K

D

D

A

I

R

K

R

R

V

V

L

A

I

V

V

V

G

G

G

A

G

G

L
x
Y

I

I

G

G

S

V

E

E

L

L

A

A

M

E

D

A

F

F

C

Q

R

R

A

K

G

G

K

K

A

E

V

V

T

V

L

L

I

I

D

D

N

V

A

V

A

D

S

T

I

C

L

L

A

A

S

G

L

Y

M

Y

P

D

P

R

E

D

V

L

S

T

S

D

R

L

L

M

Q

A

H

K

R

N

L

M

T

E

E

E

M

H

G

G

V

I

H

Q

L

L

L

A

L

F

K

G

S

E

Q

T

L

V

Q

K

G

-

L

-

E

E

K

V

T

A

D

G

S

N

G

G

-

K

I

V

Q

E

A

K

T

I

L

I

D

T

R

D

Q

K

R

N

N

E

I

Y

E

D

V

V

D

D

A

M

V

V

I

I

A

L

A

A

T

V

G

G

L

F

R

R

P

P

E

N

T

T

A

T

L

L

A

G

R

N

-

G

R

K

A

I

G

D

L

L

T

F

I

R

N

N

R

G

G

A

V

F

C

L

V

V

D

N

S

K

Y

R

L

Q

Q

E

T

T

S

S

N

I

T

P

D

G

I

V

Y

Y

A

A

L

I

G

G

D
|
D

C

C

A

A

E

T

I

I

V81 (= V81) binding
A110 (= A106) binding
S113 (≠ A109) binding
K143 (≠ R132) binding
Y170 (≠ L151) binding
D292 (= D273) binding

Sites not aligning to the query:

10 binding
12 binding
34 binding
35 binding
44 active site, Redox-active; binding ; modified: Cysteine sulfinic acid (-SO2H); C→S: Reduces specific activity about 13-fold, in vitro.
309 binding
310 binding
311 binding
436 binding

2bc0A Structural analysis of streptococcus pyogenes nadh oxidase: wild-type nox (see paper)
30% identity, 55% coverage: 69:277/377 of query aligns to 86:313/473 of 2bc0A

query
sites
2bc0A

E

E

Q

S

F

L

N

G

L

A

H

K

L

V

F

Y

P

M

Q

E

T

S

W
x
P

V
|
V

T

Q

D

S

I

I

D

D

A

Y

E

D

A

A

R

K

V

T

V

V

K

T

S

A

-

L

-

V

-

D

-

G

Q

K

N

N

N

H

Q

V

W

E

Q

T

Y

Y

D

D

K

K

L

L

V

I

L

F

A
|
A

T
|
T

G
|
G

A

S

S

Q

A

P

F

I

V

L

P

P

V

I

P

K

G

G

R

A

E

E

L

I

M

K

-

E

-

G

-

S

-

L

-

E

-

F

-

E

L

A

T

T

L

L

N

E

S

N

Q

L

Q

Q

E

F

Y

V

R
x
K

A

L

C

Y

E

Q

T

N

-

S

-

A

-

D

-

V

-

I

-

A

-

K

-

L

Q

E

L

N

R

K

D

D

A

I

R

K

R

R

V

V

L

A

I

V

V

V

G

G

G

A

G

G

L
x
Y

I
|
I

G

G

S

V

E

E

L

L

A

A

M

E

D

A

F

F

C

Q

R

R

A

K

G

G

K

K

A

E

V

V

T

V

L

L

I

I

D

D

N

V

A

V

A

D

S

T

I

C

L

L

A

A

S

G

L

Y

M

Y

P

D

P

R

E

D

V

L

S

T

S

D

R

L

L

M

Q

A

H

K

R

N

L

M

T

E

E

E

M

H

G

G

V

I

H

Q

L

L

L

A

L

F

K

G

S

E

Q

T

L

V

Q

K

G

-

L

-

E

E

K

V

T

A

D

G

S

N

G

G

-

K

I

V

Q

E

A

K

T

I

L

I

D

T

R

D

Q

K

R

N

N

E

I

Y

E

D

V

V

D

D

A

M

V

V

I

I

A

L

A

A

T

V

G

G

L

F

R

R

P

P

E

N

T

T

A

T

L

L

A

G

R

N

-

G

R

K

A

I

G

D

L

L

T

F

I

R

N

N

R

G

G

A

V

F

C

L

V

V

D

N

S

K

Y

R

L

Q

Q

E

T

T

S

S

N

I

T

P

D

G

I

V

Y

Y

A

A

L

I

G
|
G

D
|
D

C

C

A

A

E

T

I

I

binding flavin-adenine dinucleotide: P97 (≠ W80), V98 (= V81), A127 (= A106), T128 (= T107), G129 (= G108), K160 (≠ R132), Y187 (≠ L151), I188 (= I152), G308 (= G272), D309 (= D273)

Sites not aligning to the query:

active site: 28, 56, 60, 61, 331
binding flavin-adenine dinucleotide: 24, 25, 27, 28, 29, 51, 52, 61, 325, 326, 327

8pxkA Structure of nadh-dependent ferredoxin reductase, bpha4, solved at wavelength 5.76 a (see paper)
29% identity, 71% coverage: 5:273/377 of query aligns to 7:270/403 of 8pxkA

query
sites
8pxkA

I

V

V

V

I

V

I

L

G

G

S

A

G
|
G

F

L

A
|
A

A

S

R

V

Q

S

L

F

V

V

K

A

N

E

I

L

R

R

K

Q

Q

A

D

G

A

Y

T

Q

I

G

P

L

L

I

T

T

L

V

I

V

A

G

A
x
D

D
x
E

S

A

M

E

D

R

E

P

Y

Y

N

D

K
x
R

P
|
P

D

P

L

L

S

S

H
x
K

V

D

I

F

S

M

Q

A

G

H

Q

G

R

D

A

A

D

E

D

K

L

I

T

R

R

L

Q

D

T

C

A

K

G

R

E

A

F

P

A

E

E

V

Q

E

F

W

N

L

L

L

H

G

L

V

F

T

P
x
A

Q

Q

T

S

W

F

V

-

T

-

D

-

I

-

D

D

A

P

E

Q

A

A

R

H

V

T

V

V

K

A

-

L

S

S

Q

D

N

G

N

R

Q

T

W

L

Q

P

Y

Y

D

G

K

T

L

L

V

V

L

L

A

A

T
|
T

G
|
G

A

A

S

A

A

P

-

R

F

A

V

L

P

P

P

T

V

L

P

Q

G

G

R

A

E

T

L

M

M

P

L

V

-

H

T

T

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

R

A

R

C

I

E

Q

T

A

Q

G

L

L

R

R

D

P

A

Q

R

S

R

R

V

L

L

L

I

I

V

V

G

G

L

V

I
|
I

G

G

S

L

E

E

L

L

A

A

M

A

D

T

F

A

C

R

R

T

A

A

G

G

K

V

A

H

V

V

T

S

L

L

I

V

D

E

N

T

A

Q

A

P

S

R

I

L

L

M

A

S

S

R

L

A

M

A

P

P

P

A

E

T

V

L

S

A

S

D

R

F

L

V

Q

A

H

R

R

Y

L

H

T

A

E

A

M

Q

G

G

V

V

H

D

L

L

L

R

L

F

K

E

S

R

Q

S

L

V

Q

T

G

G

L

-

E

-

K

-

T

S

D

V

S

D

G

G

I

V

Q

-

A

V

T

L

L

L

D

D

R

D

Q

G

R

T

N

R

I

I

E

A

V

A

D

D

A

M

V

V

I

V

A

V

A

G

T

I

G

G

L

V

R

L

P

A

E

N

T

D

A

A

L

L

A

A

R

R

R

A

A

A

G

G

L

L

T

A

I

C

N

D

R

D

G

G

V

I

C

F

V

V

D

D

S

A

Y

Y

L

G

Q

R

T

T

S

T

N

C

T

P

D

D

I

V

Y

Y

A

A

L

L

G
|
G

D
|
D

binding flavin-adenine dinucleotide: G13 (= G11), A15 (= A13), D37 (≠ A35), E38 (≠ D36), R45 (≠ K43), P46 (= P44), K50 (≠ H48), A79 (≠ P77), T106 (= T107), G107 (= G108), R127 (≠ N126), I153 (= I152), G269 (= G272), D270 (= D273)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 286, 287, 288, 291, 317

1f3pA Ferredoxin reductase (bpha4)-nadh complex (see paper)
29% identity, 71% coverage: 5:273/377 of query aligns to 6:269/401 of 1f3pA

query
sites
1f3pA

I

V

V

V

I

V

I

L

G

G

S

A

G

G

F
x
L

A
|
A

A

S

R

V

Q

S

L

F

V

V

K

A

N

E

I

L

R

R

K

Q

Q

A

D

G

A

Y

T

Q

I

G

P

L

L

I

T

T

L

V

I
x
V

A

G

A
x
D

D
x
E

S

A

M

E

D

R

E

P

Y

Y

N

D

K
x
R

P
|
P

D

P

L

L

S

S

H

K

V

D

I

F

S

M

Q

A

G

H

Q

G

R

D

A

A

D

E

D

K

L

I

T

R

R

L

Q

D

T

C

A

K

G

R

E

A

F

P

A

E

E

V

Q

E

F

W

N

L

L

L

H

G

L

V

F

T

P
x
A

Q

Q

T

S

W

F

V

-

T

-

D

-

I

-

D

D

A

P

E

Q

A

A

R

H

V

T

V

V

K

A

-

L

S

S

Q

D

N

G

N

R

Q

T

W

L

Q

P

Y

Y

D

G

K

T

L

L

V

V

L

L

A

A

T
|
T

G
|
G

A

A

S

A

A

P

-

R

F

A

V

L

P

P

P

T

V

L

P

Q

G

G

R

A

E

T

L

M

M

P

L

V

-

H

T

T

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

R

A

R

C

I

E

Q

T

A

Q

G

L

L

R

R

D

P

A

Q

R

S

R

R

V

L

L

L

I

I

V
|
V

G
|
G

G

G

G
|
G

L
x
V

I
|
I

G

G

S

L

E
|
E

L

L

A

A

M

A

D

T

F

A

C

R

R

T

A

A

G

G

K

V

A

H

V

V

T

S

L

L

I

V

D
x
E

N
x
T

A

Q

A

P

S

R

I

L

L

M

A

S

S
x
R

L

A

M

A

P

P

P

A

E

T

V

L

S

A

S

D

R

F

L

V

Q

A

H

R

R

Y

L

H

T

A

E

A

M

Q

G

G

V

V

H

D

L

L

L

R

L

F

K

E

S

R

Q

S

L

V

Q

T

G

G

L

-

E

-

K

-

T

S

D

V

S

D

G

G

I

V

Q

-

A

V

T

L

L

L

D

D

R

D

Q

G

R

T

N

R

I

I

E

A

V

A

D

D

A

M

V

V

I

V

A

V

A
x
G

T
x
I

G
|
G

L
x
V

R

L

P

A

E

N

T

D

A

A

L

L

A

A

R

R

R

A

A

A

G

G

L

L

T

A

I

C

N

D

R

D

G

G

V

I

C

F

V

V

D

D

S

A

Y

Y

L

G

Q

R

T

T

S

T

N

C

T

P

D

D

I

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L13 (≠ F12), R44 (≠ K43), P45 (= P44)
binding flavin-adenine dinucleotide: A14 (= A13), V34 (≠ I33), D36 (≠ A35), E37 (≠ D36), R44 (≠ K43), P45 (= P44), A78 (≠ P77), T105 (= T107), G106 (= G108), R126 (≠ N126), G268 (= G272), D269 (= D273)
binding nicotinamide-adenine-dinucleotide: V147 (= V147), G148 (= G148), G150 (= G150), V151 (≠ L151), I152 (= I152), E155 (= E155), E171 (≠ D171), T172 (≠ N172), R179 (≠ S179), G230 (≠ A234), I231 (≠ T235), G232 (= G236), V233 (≠ L237)

Sites not aligning to the query:

active site: 291
binding flavin-adenine dinucleotide: 285, 286, 287, 290, 316
binding nicotinamide-adenine-dinucleotide: 285, 316, 317

3nt6A Structure of the shewanella loihica pv-4 nadh-dependent persulfide reductase c43s/c531s double mutant (see paper)
28% identity, 75% coverage: 48:331/377 of query aligns to 48:367/565 of 3nt6A

query
sites
3nt6A

H

H

V

I

I

S

S

G

Q

E

G

I

Q

A

R

Q

A

R

D

S

D

A

L

L

T

V

R

L

Q

Q

T

T

A

P

G

E

E

S

F

F

A

K

E

A

Q

R

F

F

N

N

L

V

H

E

L

V

F

R

P

V

Q

K

T

H

W
x
E

V
|
V

T

V

D

A

I

I

D

D

A

R

E

A

A

A

R

K

V

L

V

V

-

T

-

V

-

R

-

L

K

D

S

G

Q

S

N

E

N

Y

Q

Q

W

E

Q

S

Y

Y

D

D

K

T

L

L

V

L

L

L

A
x
S

T
x
P

G
|
G

A

A

S

A

A

P

F

I

V

V

P

P

V

I

P

P

G

G

R

V

E

D

L

N

M

P

L

L

T

T

L

H

-

S

-
x
L

-
x
R

N

N

S

I

Q

P

Q

D

E

M

Y

D

R

R

A

I

C

L

E

Q

T

T

-

I

Q

Q

L

M

R

N

D

N

A

V

R

E

R

H

V

A

L

T

I

V

V

V

G

G

L
x
F

I

I

G

G

S

L

E

E

L

M

A

M

M

E

D

S

F

L

C

H

R

H

A

L

G

G

K

I

A

K

V

T

T

T

L

L

I

L

D

E

N

L

A

A

A

D

S

Q

I

V

L

M

A

T

S

P

L

V

M

-

P

D

P

R

E

E

V

M

S

A

S

G

R

F

L

A

Q

H

H

Q

R

A

L

I

T

R

E

D

M

Q

G

G

V

V

H

D

L

L

L

R

L

L

K

G

S

T

-

A

-

L

-

S

-

E

-

V

-

S

-

Y

Q

Q

L

V

Q

Q

G

T

L

H

E

V

K

A

T

S

D

D

S

A

G

A

-

G

-

E

-

D

-

T

-

A

-

H

-

Q

-

H

-

I

-

K

-

G

-

H

I

L

Q

S

A

L

T

T

L

L

D

S

R

N

Q

G

R

E

N

L

I

L

E

E

V

T

D

D

A

L

V

L

I

I

A

M

A

A

T

I

G

G

L

V

R

R

P

P

E

E

T

T

A

Q

L

L

A

A

R

R

R

D

A

A

G

G

L

L

T

A

I

I

N

G

R

E

-

L

-

G

G

G

V

I

C

K

V

V

D

N

S

A

Y

M

L

M

Q

Q

T

T

S

S

N

D

T

P

D

A

I

I

Y

Y

A

A

L

V

G
|
G

D
|
D

C

A

A

V

E

E

-

E

-

Q

-

D

-

F

I

V

N

T

G

G

Q

Q

-

A

-

C

V

L

L

V

P
|
P

F
x
L

L
x
A

Q

G

P

P

I

A

Q
x
N

L

R

S

Q

A

G

M
x
R

V

M

L

A

A

A

K

D

N

N

L

M

L

F

G

G

N

R

N

E

T

E

P

R

L

Y

K

Q

L

G

P

T

A

Q

M

G

L

T

V

A

K

I

I

C

K

K

T

V

P

F

E

D

L

L

P

A

L

V

H

G

L

A

A

T

G

G

E

K

T

N

Q

E

R

K

Q

Q

active site: N325 (≠ Q289)
binding coenzyme a: A321 (≠ L285), N325 (≠ Q289), R329 (≠ M293)
binding flavin-adenine dinucleotide: E80 (≠ W80), V81 (= V81), S112 (≠ A106), P113 (≠ T107), G114 (= G108), L133 (vs. gap), R134 (vs. gap), F161 (≠ L151), G302 (= G272), D303 (= D273), P319 (= P283), L320 (≠ F284), A321 (≠ L285)

Sites not aligning to the query:

active site: 11, 38, 42, 43, 471, 472
binding coenzyme a: 11, 18, 19, 22, 39, 42, 43, 459, 462, 463, 466, 534, 535
binding flavin-adenine dinucleotide: 7, 8, 10, 11, 12, 33, 34, 42, 43

2yvjA Crystal structure of the ferredoxin-ferredoxin reductase (bpha3-bpha4)complex (see paper)
29% identity, 71% coverage: 5:273/377 of query aligns to 6:269/402 of 2yvjA

query
sites
2yvjA

I

V

V

V

I

V

I

L

G
|
G

S

A

G
|
G

F
x
L

A

A

A

S

R

V

Q

S

L

F

V

V

K

A

N

E

I

L

R

R

K

Q

Q

A

D

G

A

Y

T

Q

I

G

P

L

L

I

T

T

L

V

I

V

A
x
G

A
x
D

D
x
E

S

A

M

E

D

R

E

P

Y

Y

N

D

K
x
R

P
|
P

D

P

L

L

S

S

H

K

V

D

I

F

S

M

Q

A

G

H

Q

G

R

D

A

A

D

E

D

K

L

I

T

R

R

L

Q

D

T

C

A

K

G

R

E

A

F

P

A

E

E

V

Q

E

F

W

N

L

L

L

H

G

L

V

F

T

P
x
A

Q

Q

T

S

W

F

V

-

T

-

D

-

I

-

D

D

A

P

E

Q

A

A

R

H

V

T

V

V

K

A

-

L

S

S

Q

D

N

G

N

R

Q

T

W

L

Q

P

Y

Y

D

G

K

T

L

L

V

V

L

L

A

A

T
|
T

G
|
G

A

A

S

A

A

P

-

R

F

A

V

L

P

P

P

T

V

L

P

Q

G

G

R

A

E

T

L

M

M

P

L

V

-

H

T

T

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

R

A

R

C

I

E

Q

T

A

Q

G

L

L

R

R

D

P

A

Q

R

S

R

R

V

L

L

L

I

I

V
|
V

G
|
G

L

V

I
|
I

G

G

S

L

E

E

L

L

A

A

M

A

D

T

F

A

C

R

R

T

A

A

G

G

K

V

A

H

V

V

T

S

L

L

I
x
V

D
x
E

N
x
T

A

Q

A

P

S

R

I

L

L

M

A

S

S
x
R

L

A

M

A

P

P

P

A

E

T

V

L

S

A

S

D

R

F

L

V

Q

A

H

R

R

Y

L

H

T

A

E

A

M

Q

G

G

V

V

H

D

L

L

L

R

L

F

K

E

S

R

Q

S

L

V

Q

T

G

G

L

-

E

-

K

-

T

S

D

V

S

D

G

G

I

V

Q

-

A

V

T

L

L

L

D

D

R

D

Q

G

R

T

N

R

I

I

E

A

V

A

D

D

A

M

V

V

I

V

A

V

A
x
G

T
x
I

G
|
G

L
x
V

R

L

P

A

E

N

T

D

A

A

L

L

A

A

R

R

R

A

A

A

G

G

L

L

T

A

I

C

N

D

R

D

G

G

V

I

C

F

V

V

D

D

S

A

Y

Y

L

G

Q

R

T

T

S

T

N

C

T

P

D

D

I

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L13 (≠ F12), R44 (≠ K43), P45 (= P44)
binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), G35 (≠ A34), D36 (≠ A35), E37 (≠ D36), R44 (≠ K43), P45 (= P44), A78 (≠ P77), T105 (= T107), G106 (= G108), R126 (≠ N126), G268 (= G272), D269 (= D273)
binding 1,4-dihydronicotinamide adenine dinucleotide: V147 (= V147), G148 (= G148), G149 (= G149), G150 (= G150), I152 (= I152), V170 (≠ I170), E171 (≠ D171), T172 (≠ N172), R179 (≠ S179), G230 (≠ A234), I231 (≠ T235), G232 (= G236), V233 (≠ L237)

Sites not aligning to the query:

active site: 291
binding flavin-adenine dinucleotide: 286, 287, 290, 316
binding 1,4-dihydronicotinamide adenine dinucleotide: 285

2gr2A Crystal structure of ferredoxin reductase, bpha4 (oxidized form)
29% identity, 71% coverage: 5:273/377 of query aligns to 5:268/401 of 2gr2A

query
sites
2gr2A

I

V

V

V

I

V

I

L

G

G

S

A

G
|
G

F
x
L

A
|
A

A

S

R

V

Q

S

L

F

V

V

K

A

N

E

I

L

R

R

K

Q

Q

A

D

G

A

Y

T

Q

I

G

P

L

L

I

T

T

L

V

I

V

A

G

A
x
D

D
x
E

S

A

M

E

D

R

E

P

Y

Y

N

D

K
x
R

P
|
P

D

P

L

L

S

S

H
x
K

V

D

I

F

S

M

Q

A

G

H

Q

G

R

D

A

A

D

E

D

K

L

I

T

R

R

L

Q

D

T

C

A

K

G

R

E

A

F

P

A

E

E

V

Q

E

F

W

N

L

L

L

H

G

L

V

F

T

P
x
A

Q

Q

T

S

W

F

V

-

T

-

D

-

I

-

D

D

A

P

E

Q

A

A

R

H

V

T

V

V

K

A

-

L

S

S

Q

D

N

G

N

R

Q

T

W

L

Q

P

Y

Y

D

G

K

T

L

L

V

V

L

L

A

A

T
|
T

G
|
G

A

A

S

A

A

P

-
x
R

F

A

V

L

P

P

P

T

V

L

P

Q

G

G

R

A

E

T

L

M

M

P

L

V

-

H

T

T

L

L

N
x
R

S

T

Q

L

Q

E

E

D

Y

A

R

R

A

R

C

I

E

Q

T

A

Q

G

L

L

R

R

D

P

A

Q

R

S

R

R

V

L

L

L

I

I

V
|
V

G
|
G

G

G

G
|
G

L
x
V

I
|
I

G

G

S

L

E

E

L

L

A

A

M

A

D

T

F

A

C

R

R

T

A

A

G

G

K

V

A

H

V

V

T

S

L

L

I

V

D
x
E

N
x
T

A

Q

A

P

S

R

I

L

L

M

A

S

S
x
R

L

A

M

A

P

P

P

A

E

T

V

L

S

A

S

D

R

F

L

V

Q

A

H

R

R

Y

L

H

T

A

E

A

M

Q

G

G

V

V

H

D

L

L

L

R

L

F

K

E

S

R

Q

S

L

V

Q

T

G

G

L

-

E

-

K

-

T

S

D

V

S

D

G

G

I

V

Q

-

A

V

T

L

L

L

D

D

R

D

Q

G

R

T

N

R

I

I

E

A

V

A

D

D

A

M

V

V

I

V

A

V

A
x
G

T
x
I

G
|
G

L

V

R

L

P

A

E

N

T

D

A

A

L

L

A

A

R

R

R

A

A

A

G

G

L

L

T

A

I

C

N

D

R

D

G

G

V

I

C

F

V

V

D

D

S

A

Y

Y

L

G

Q

R

T

T

S

T

N

C

T

P

D

D

I

V

Y

Y

A

A

L

L

G
|
G

D
|
D

active site: L12 (≠ F12), R43 (≠ K43), P44 (= P44)
binding adenosine-5-diphosphoribose: R109 (vs. gap), V146 (= V147), G147 (= G148), G149 (= G150), V150 (≠ L151), I151 (= I152), E170 (≠ D171), T171 (≠ N172), R178 (≠ S179), G229 (≠ A234), I230 (≠ T235), G231 (= G236)
binding flavin-adenine dinucleotide: G11 (= G11), A13 (= A13), D35 (≠ A35), E36 (≠ D36), R43 (≠ K43), P44 (= P44), K48 (≠ H48), A77 (≠ P77), T104 (= T107), G105 (= G108), R125 (≠ N126), G267 (= G272), D268 (= D273)

Sites not aligning to the query:

active site: 290
binding adenosine-5-diphosphoribose: 284
binding flavin-adenine dinucleotide: 285, 286, 289, 315

Query Sequence

>16795 FitnessBrowser__Keio:16795
MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
RQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQATLDRQRNIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
SEDRMKEAFGLLKTLPM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory