SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 17826 FitnessBrowser__Keio:17826 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P05793 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 2; Ketol-acid reductoisomerase type II; EC 1.1.1.86 from Escherichia coli (strain K12) (see 5 papers)
100% identity, 100% coverage: 1:491/491 of query aligns to 1:491/491 of P05793

query
sites
P05793

M
|
M

A

A

N

N

Y

Y

F

F

N

N

T

T

L

L

N

N

L

L

R

R

Q

Q

L

L

A

A

Q

Q

L

L

G

G

K

K

C

C

R

R

F

F

M

M

G

G

R

R

D

D

E

E

F

F

A

A

D

D

G

G

A

A

S

S

Y

Y

L

L

Q

Q

G

G

K

K

V

V

I

I

V

V

G

G

C
|
C

G
|
G

A
|
A

Q
|
Q

G

G

L

L

N

N

Q

Q

G

G

L

L

N

N

M

M

R

R

D

D

S

S

G

G

L

L

D

D

I

I

S

S

Y

Y

A

A

L

L

R
|
R

K
|
K

E

E

A
|
A

I

I

A

A

E

E

K
|
K

R
|
R

A

A

S
|
S

W

W

R

R

K

K

A

A

T

T

E

E

N

N

G

G

F

F

K

K

V

V

G

G

T

T

Y

Y

E

E

L

L

I

I

P

P

Q

Q

A

A

D

D

L

L

V

V

I

I

N

N

L

L

T

T

P

P

D
|
D

K
|
K

Q
|
Q

H

H

S

S

D

D

V

V

R

R

T

T

V

V

Q

Q

P

P

L

L

M

M

K

K

D

D

G

G

A

A

L

L

G

G

Y

Y

S

S

H
|
H

G

G

F

F

N

N

I

I

V

V

E

E

V

V

G

G

E

E

Q

Q

I

I

R

R

K

K

D

D

I

I

T

T

V

V

M

M

V

V

A

A

P

P

K
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K

C

C

P

P

G

G

T

T

E

E

V

V

R

R

E

E

Y

Y

K

K

R

R

G

G

F

F

G

G

V

V

P

P

T

T

L

L

I

I

A

A

V

V

H

H

P

P

E

E

N

N

D

D

P

P

K

K

G

G

E

E

G

G

M

M

A

A

I

I

A

A

K

K

A

A

W

W

A

A

T

T

G

G

H

H

R

R

A

A

G

G

V

V

L

L

E

E

S

S

F

F

V

V

A

A

E
|
E

V

V

K

K

S

S

D
|
D

L

L

M

M

G

G

E
|
E

Q

Q

T

T

I

I

L

L

C

C

G

G

M

M

L

L

Q

Q

A

A

G

G

S

S

L

L

C

C

F

F

D

D

K

K

L

L

V

V

E

E

G

G

T

T

D

D

P

P

A

A

Y

Y

A

A

E

E

K

K

L

L

I

I

Q

Q

F

F

G

G

W

W

E

E

T

T

I

I

T

T

E

E

A

A

L

L

K

K

Q

Q

G

G

I

I

T

T

L

L

M

M

D

D

R

R

L

L

S

S

N

N

P

P

A

A

K

K

L

L

R

R

A

A

Y

Y

A

A

L

L

S

S

E

E

Q

Q

L

L

K

K

E

E

I

I

M

M

A

A

P

P

L

L

F

F

Q

Q

K

K

H

H

M

M

D

D

I

I

S

S

G

G

E

E

F

F

S

S

G

G

M

M

A

A

D

D

W

W

A

A

N

N

D

D

K

K

L

L

T

T

W

W

R

R

E

E

T

T

G

G

K

K

T

T

A

A

F

F

E

E

T

T

A

A

P

P

Q

Q

Y

Y

E

E

G

G

K

K

I

I

G

G

E

E

Q

Q

E

E

Y

Y

F

F

D

D

K

K

G

G

V

V

L

L

M

M

I

I

A

A

M

M

V

V

K

K

A

A

G

G

V

V

E

E

L

L

A

A

F

F

E

E

T

T

M

M

V

V

D

D

S

S

G

G

I

I

E

E

S

S

A

A

Y

Y

E
|
E

S

S

L

L

H

H

E
|
E

L

L

P

P

L

L

I

I

A

A

N

N

T

T

I

I

A

A

R

R

K

K

R

R

L

L

Y

Y

E

E

M

M

N

N

V

V

I

I

S
|
S

D

D

T

T

A

A

E

E

Y

Y

G

G

N

N

Y

Y

L

L

F

F

S

S

Y

Y

A

A

C

C

V

V

P

P

L

L

K

K

P

P

F

F

M

M

A

A

E

E

L

L

Q

Q

P

P

G

G

D

D

L

L

G

G

K

K

A

A

I

I

P

P

E

E

G

G

A

A

V

V

D

D

N

N

G

G

Q

Q

L

L

R

R

D

D

V

V

N

N

E

E

A

A

I

I

R

R

S

S

H

H

A

A

I

I

E

E

Q

Q

V

V

G

G

K

K

L

L

R

R

G

G

Y

Y

M

M

T

T

D

D

M

M

K

K

R

R

I

I

A

A

V

V

A

A

G

G

M1 (= M1) modified: Initiator methionine, Removed
CGAQ 45:48 (= CGAQ 45:48) binding NADP(+)
R68 (= R68) mutation to D: Inversion of cofactor specificity from NADPH to NADH; when associated with L-69, V-75 and D-76.; mutation to Q: 18-fold decrease of the catalytic efficiency and 3-fold decrease of the affinity for NADPH.
K69 (= K69) mutation to L: Does not significantly alter the affinity for NADPH. Slight increase of the catalytic efficiency. Inversion of cofactor specificity from NADPH to NADH; when associated with D-68, V-75 and D-76.
A71 (= A71) mutation to S: 7- and 2.5-fold increase of the reductoisomerase activity with NADH and NADPH, respectively.
K75 (= K75) mutation to Q: 13-fold decrease of the catalytic efficiency and 3-fold increase of the affinity for NADPH.; mutation to V: Inversion of cofactor specificity from NADPH to NADH; when associated with D-68, L-69 and D-76.
R76 (= R76) binding NADP(+); mutation to D: 3-fold increase of the reductoisomerase activity with NADH and slight decrease of the reductoisomerase activity with NADPH.; mutation to D: Strong increase of catalytic efficiency and 2.5-fold increase of the affinity for NADH. 4-fold decrease of the catalytic efficiency and strong decrease of the affinity for NADPH. Inversion of cofactor specificity from NADPH to NADH; when associated with D-68, L-69 and V-75.; mutation to Q: 20-fold decrease of the catalytic efficiency and 5-fold decrease of the affinity for NADPH.
S78 (= S78) binding NADP(+); mutation to D: 12-fold increase of the reductoisomerase activity with NADH and slight decrease of the reductoisomerase activity with NADPH.
DKQ 108:110 (= DKQ 108:110) binding NADP(+)
Q110 (= Q110) mutation Q->V,A: 12- and 2-fold increase of the reductoisomerase activity with NADH and NADPH, respectively.
H132 (= H132) mutation to K: Loss of reductoisomerase activity.; mutation to Q: Loss of reductoisomerase activity. The reductase activity with 3-hydroxypyruvate and HMKB is nearly normal, and the isomerase activity decreases 24-fold.
K155 (= K155) mutation K->E,Q: Loss of reductoisomerase activity.; mutation to R: Loss of reductoisomerase activity. The reductase activity with 3-hydroxypyruvate and HMKB is nearly normal, and the isomerase activity decreases 40-fold.
E213 (= E213) mutation to D: Loss of reductoisomerase activity. 1.5-fold decrease of the reductase activity with 3-hydroxypyruvate and the isomerase activity decreases 48-fold.
D217 (= D217) binding Mg(2+); binding Mg(2+); mutation D->E,N: Loss of reductoisomerase activity.
E221 (= E221) mutation E->D,Q: Loss of reductoisomerase activity.
E389 (= E389) binding Mg(2+); mutation to D: Loss of reductoisomerase activity. 1.5-fold decrease of the reductase activity with 3-hydroxypyruvate and the isomerase activity decreases 4-fold.; mutation to Q: Loss of reductoisomerase activity.
E393 (= E393) binding Mg(2+); mutation to D: Loss of reductoisomerase activity. The reductase activity with HMKB is nearly normal.
S414 (= S414) mutation to A: Loss of reductoisomerase activity. The isomerase activity decreases 15-fold.; mutation to T: Loss of reductoisomerase activity. The isomerase activity decreases 24-fold.

3ulkA E. Coli ketol-acid reductoisomerase in complex with NADPH and mg2+ (see paper)
100% identity, 100% coverage: 1:489/491 of query aligns to 1:489/489 of 3ulkA

query
sites
3ulkA

M

M

A

A

N

N

Y

Y

F

F

N

N

T

T

L

L

N

N

L

L

R

R

Q

Q

L

L

A

A

Q

Q

L

L

G

G

K

K

C

C

R

R

F

F

M

M

G

G

R

R

D

D

E

E

F

F

A

A

D

D

G

G

A

A

S

S

Y

Y

L

L

Q

Q

G

G

K

K

V

V

I

I

V

V

G

G

C

C

G
|
G

A
|
A

Q
|
Q

G

G

L

L

N

N

Q

Q

G

G

L

L

N

N

M

M

R

R

D

D

S

S

G

G

L

L

D

D

I

I

S

S

Y

Y

A

A

L

L

R
|
R

K

K

E

E

A

A

I

I

A

A

E

E

K

K

R
|
R

A

A

S
|
S

W

W

R

R

K

K

A

A

T

T

E

E

N

N

G

G

F

F

K

K

V

V

G

G

T

T

Y

Y

E

E

L

L

I

I

P

P

Q

Q

A

A

D

D

L

L

V

V

I

I

N

N

L
|
L

T
|
T

P
|
P

D
|
D

K

K

Q
|
Q

H

H

S

S

D

D

V
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V

V

V

R

R

T

T

V

V

Q

Q

P

P

L

L

M

M

K

K

D

D

G

G

A

A

L

L

G

G

Y

Y

S

S

H

H

G

G

F

F

N

N

I

I

V

V

E

E

V

V

G

G

E

E

Q

Q

I

I

R

R

K

K

D

D

I

I

T

T

V

V

M

M

V

V

A

A

P

P

K
|
K

C

C

P

P

G

G

T

T

E

E

V

V

R

R

E

E

Y

Y

K

K

R

R

G

G

F

F

G

G

V

V

P

P

T

T

L

L

I

I

A

A

V

V

H

H

P

P

E

E

N

N

D

D

P

P

K

K

G

G

E

E

G

G

M

M

A

A

I

I

A

A

K

K

A

A

W

W

A

A

T

T

G

G

H

H

R

R

A

A

G

G

V

V

L

L

E

E

S

S

F

F

V

V

A

A

E

E

V

V

K

K

S

S

D
|
D

L

L

M

M

G

G

E
|
E

Q

Q

T

T

I

I

L

L

C

C

G

G

M

M

L

L

Q

Q

A

A

G

G

S

S

L

L

C

C

F

F

D

D

K

K

L

L

V

V

E

E

G

G

T

T

D

D

P

P

A

A

Y

Y

A

A

E

E

K

K

L

L

I

I

Q

Q

F

F

G

G

W

W

E

E

T

T

I

I

T

T

E

E

A

A

L

L

K

K

Q

Q

G

G

I

I

T

T

L

L

M

M

D

D

R

R

L

L

S

S

N

N

P

P

A

A

K

K

L

L

R

R

A

A

Y

Y

A

A

L

L

S

S

E

E

Q

Q

L

L

K

K

E

E

I

I

M

M

A

A

P

P

L

L

F

F

Q

Q

K

K

H

H

M

M

D

D

I

I

S

S

G

G

E

E

F

F

S

S

G

G

M

M

A

A

D

D

W

W

A

A

N

N

D

D

K

K

L

L

T

T

W

W

R

R

E

E

T

T

G

G

K

K

T

T

A

A

F

F

E

E

T

T

A

A

P

P

Q

Q

Y

Y

E

E

G

G

K

K

I

I

G

G

E

E

Q

Q

E

E

Y

Y

F

F

D

D

K

K

G

G

V

V

L

L

M

M

I

I

A

A

M

M

V

V

K

K

A

A

G

G

V

V

E

E

L

L

A

A

F

F

E

E

T

T

M

M

V

V

D

D

S

S

G

G

I

I

E

E

S

S

A

A

Y

Y

E
|
E

S

S

L

L

H

H

E
|
E

L

L

P

P

L

L

I

I

A

A

N

N

T

T

I

I

A

A

R

R

K

K

R

R

L

L

Y

Y

E

E

M

M

N

N

V

V

I

I

S

S

D

D

T

T

A

A

E

E

Y

Y

G

G

N

N

Y

Y

L

L

F

F

S

S

Y

Y

A

A

C

C

V

V

P

P

L

L

K

K

P

P

F

F

M

M

A

A

E

E

L

L

Q

Q

P

P

G

G

D

D

L

L

G

G

K

K

A

A

I

I

P

P

E

E

G

G

A

A

V

V

D

D

N

N

G

G

Q

Q

L

L

R

R

D

D

V

V

N

N

E

E

A

A

I

I

R

R

S

S

H

H

A

A

I

I

E

E

Q

Q

V

V

G

G

K

K

L

L

R

R

G

G

Y

Y

M

M

T

T

D

D

M

M

K

K

R

R

I

I

A

A

V

V

active site: K155 (= K155), D217 (= D217), E221 (= E221)
binding magnesium ion: D217 (= D217), E389 (= E389), E393 (= E393)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G46 (= G46), A47 (= A47), Q48 (= Q48), R68 (= R68), R76 (= R76), S78 (= S78), L105 (= L105), T106 (= T106), P107 (= P107), D108 (= D108), Q110 (= Q110), V114 (= V114)

5e4rA Crystal structure of domain-duplicated synthetic class ii ketol-acid reductoisomerase 2ia_kari-dd (see paper)
31% identity, 91% coverage: 35:481/491 of query aligns to 14:462/466 of 5e4rA

query
sites
5e4rA

L

I

Q

K

G

N

K

K

K

T

V

I

V

A

I

I

V

L

G

G

C

Y

G
|
G

A
x
S

Q
|
Q

G

G

L

R

N

A

Q

W

G

A

L

L

N

N

M

L

R

R

D

D

S

S

G

G

L

L

D

N

I

V

S

V

Y

V

A

G

L

L

R

E

K

R

E

Q

A

G

I

-

A

-

E

-

K

-

R

-

A

D

S
|
S

W

W

R

R

K

R

A

A

T

I

E

D

N

D

G

G

F

F

K

K

V

P

G

M

T

Y

Y

T

E

K

E

D

L

A

I

V

P

A

Q

I

A

A

D

D

L

I

V

I

I

V

N

F

L
|
L

T
x
V

P
|
P

D
|
D

K

M

-
x
V

Q

Q

H

K

S

S

D

L

V

W

V

L

R

N

T

S

V

V

Q

K

P

D

L

F

M

M

K

K

D

K

G

G

A

A

A

D

L

L

G

V

Y

F

S
x
A

H
|
H

G

G

F

F

N

N

I

I

V

H

E

F

V

K

G

I

E

I

Q

E

I

P

R

P

K

K

D

D

I

S

T

D

V

V

V

Y

M

M

V

I

A

A

P

P

K
|
K

C

S

P
|
P

G

G

T

P

E

I

V

V

R

R

E

R

E

S

Y

Y

K

E

R

M

G

G

F

G

G

G

V

V

P

P

T

A

L

L

I

V

A

A

V

V

H

Y

P

-

E

-

N

Q

D

N

P

V

K

S

G

G

E

E

G

A

M

L

A

Q

I

K

A

A

K

L

A

A

W

I

A

A

A

K

A

G

T

I

G

G

G

C

H

A

R

R

A

A

G

G

V

V

L

I

E

E

S

S

S

T

F

F

V

K

A

E

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

V

I

I

L

L

C

V

G

G

M

G

L

I

Q

M

A

E

G

L

S

I

L

K

L

A

C

S

F

F

D

E

K

T

L

L

V

V

E

E

G

G

T

Y

D

Q

P

P

-

E

-

V

A

A

Y

Y

A

F

E

E

K

T

L

V

I

N

Q

E

F

L

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

E

G

K

G

G

I

L

T

T

L

G

M

M

M

L

D

R

R

A

L

V

S

S

N

D

P

T

A

A

K

K

-

Y

-

G

-

I

-

T

L

V

R

G

A

K

Y

F

A

I

L

I

S

D

E

K

Q

S

L

V

K

R

E

D

I

K

M

M

A

K

P

I

L

V

F

L

Q

E

K

R

H

-

M

-

D

-

I

I

I

R

S

S

G

G

E

E

F

F

S

A

S

R

G

E

M

W

M

I

A

K

D

E

W

Y

A

E

N

R

D

G

D

M

K

P

K

T

L

V

L

F

T

K

W

E

R

L

E

S

E

E

T

L

G

E

K

G

T

S

A

T

F

I

E

E

T

T

A

V

P

G

Q

R

Y

-

E

-

G

-

K

K

I

L

G

R

E

E

Q

M

E

M

Y

F

-

R

-

G

-

M

-

L

F

F

D

G

K

E

G

Q

V

V

L

I

M

L

I

V

A

G

M

G

V

I

K

M

A

E

G

L

V

I

E

K

L

A

A

S

F

F

E

E

T

T

M

L

V

V

D

E

S

E

G

G

I

Y

I

Q

E

P

E

E

S

V

A

A

Y

Y

Y

F

E

E

S

T

L

V

H

N

E

E

L

L

P

K

L

L

I

I

A

V

N

D

T

L

I

I

A

Y

R

E

K

K

R

G

L

L

Y

T

E

G

M

M

N

L

V

R

V
x
A

I
x
V

S
|
S

D

D

T

T

A
|
A

E

K

Y

Y

-

G

-

I

-

T

-

V

G

G

N

K

Y

F

L

I

F

I

S

D

Y

K

A

S

C

V

V

R

P

D

L

K

L

M

K

K

P

I

F

V

M

L

A

E

E

R

L

I

Q

R

P

S

G

G

D

E

L

F

G

A

K

R

A

E

-

W

I

I

P

K

E

E

G

-

A

-

V

Y

D

E

N

R

G

G

Q

M

-

P

-

T

-

V

L

F

R

K

D

E

V

L

N

S

E

E

A

-

I

L

R

E

S

G

H

S

A

T

I

I

E

E

Q

T

V

V

G

G

K

R

K

K

L

L

R

R

G

E

Y

M

M

M

active site: K130 (= K155), D190 (= D217), E194 (= E221)
binding oxo(propan-2-ylamino)acetic acid: P132 (= P157), D190 (= D217), E194 (= E221), V388 (≠ I413), S389 (= S414), A392 (= A417)
binding magnesium ion: D190 (= D217), E194 (= E221)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G25 (= G46), S26 (≠ A47), Q27 (= Q48), S52 (= S78), L79 (= L105), V80 (≠ T106), P81 (= P107), D82 (= D108), V84 (vs. gap), A106 (≠ S131), H107 (= H132), P132 (= P157), A387 (≠ V412), V388 (≠ I413), S389 (= S414)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
35% identity, 57% coverage: 35:312/491 of query aligns to 15:291/342 of 8upqB

query
sites
8upqB

L

I

Q

K

G

S

K

K

V

V

V

A

I

I

V

I

G

G

C

F

G

G

A

S

Q

Q

G

G

L

H

N

A

Q

H

G

A

L

M

N

N

M

L

R

R

D

D

S

N

G

G

L

V

D

N

I

V

S

T

Y

I

A

G

L

L

R

R

K

-

E

-

A

-

I

-

A

-

E

E

K

G

R

S

A

V

S

S

W

A

R

V

K

K

A

A

T

K

E

N

N

A

G

G

F

F

K

E

V

V

G

M

T

S

Y

V

E

S

E

E

L

A

I

S

P

K

Q

I

A

A

D

D

L

V

V

I

I

M

N

I

L

L

T

A

P

P

D

D

K

E

Q

I

H

Q

S

A

D

D

V

I

V

F

R

N

T

V

-

E

V

I

Q

K

P

P

L

N

M

L

K

S

D

E

G

G

A

K

A

A

L

I

G

A

Y

F

S

A

H

H

G

G

F

F

N

N

I

I

V

H

E

Y

V

G

G

Q

E

I

Q

V

I

V

R

P

K

K

D

G

I

V

T

D

V

V

V

I

M

M

V

I

A

A

P

P

K

K

C
x
A

P
|
P

G

G

T

H

E

T

V

V

R

R

E

N

E

E

Y

F

K

T

R

L

G

G

F

G

G

G

V

T

P

P

T

C

L

L

I

I

A

A

V

I

H

H

P

Q

E

D

N

E

D

S

P

K

K

N

G

A

E

K

G

N

M

L

A

A

I

L

A

S

K

-

A

-

W

Y

A

A

A

S

A

A

T

I

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

A

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

M

G

L

L

Q

S

A

A

G

L

S

I

L

Q

L

A

C

G

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

E

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

L

I

H

Q
x
E

F

M

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

Q

G

G

I

I

T

A

L

D

M

M

M

R

D

Y

R

S

L

I

S
|
S

N

N

P

T

A
|
A

K

E

L

Y

R

G

A

D

Y

Y

-

I

-

T

-

G

-

P

-

K

A

I

L

I

S

T

E

E

Q

E

L

T

K

K

E

K

I

A

M

M

A

K

P

G

L

V

F

L

Q

K

K

D

H

I

M

Q

D

N

D

G

I

V

I

F

S

A

G

K

E

D

F

F

binding magnesium ion: D191 (= D217), E195 (= E221)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (≠ C156), P133 (= P157), D191 (= D217), E195 (= E221), E231 (≠ Q255), S252 (= S278), A255 (= A281)

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
35% identity, 57% coverage: 35:312/491 of query aligns to 11:287/323 of 8swmA

query
sites
8swmA

L

I

Q

K

G

S

K

K

V

V

V

A

I

I

V

I

G

G

C

F

G

G

A

S

Q

Q

G

G

L

H

N

A

Q

H

G

A

L

M

N

N

M

L

R

R

D

D

S

N

G

G

L

V

D

N

I

V

S

T

Y

I

A

G

L

L

R

R

K

-

E

-

A

-

I

-

A

-

E

E

K

G

R

S

A

V

S

S

W

A

R

V

K

K

A

A

T

K

E

N

N

A

G

G

F

F

K

E

V

V

G

M

T

S

Y

V

E

S

E

E

L

A

I

S

P

K

Q

I

A

A

D

D

L

V

V

I

I

M

N

I

L

L

T

A

P

P

D

D

K

E

Q

I

H

Q

S

A

D

D

V

I

V

F

R

N

T

V

-

E

V

I

Q

K

P

P

L

N

M

L

K

S

D

E

G

G

A

K

A

A

L

I

G

A

Y

F

S

A

H

H

G

G

F

F

N

N

I

I

V

H

E

Y

V

G

G

Q

E

I

Q

V

I

V

R

P

K

K

D

G

I

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T

D

V

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I

M

M

V

I

A

A

P

P

K

K

C

A

P

P

G

G

T

H

E

T

V

V

R

R

E

N

E

E

Y

F

K

T

R

L

G

G

F

G

G

G

V

T

P

P

T

C

L

L

I

I

A

A

V

I

H

H

P

Q

E

D

N

E

D

S

P

K

K

N

G

A

E

K

G

N

M

L

A

A

I

L

A

S

K

-

A

-

W

Y

A

A

A

S

A

A

T

I

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

A

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

M

G

L

L

Q

S

A

A

G

L

S

I

L

Q

L

A

C

G

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

E

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

L

I

H

Q

E

F

M

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

Q

G

G

I

I

T

A

L

D

M

M

M

R

D

Y

R

S

L

I

S

S

N

N

P

T

A

A

K

E

L

Y

R

G

A

D

Y

Y

-

I

-

T

-

G

-

P

-

K

A

I

L

I

S

T

E

E

Q

E

L

T

K

K

E

K

I

A

M

M

A

K

P

G

L

V

F

L

Q

K

K

D

H

I

M

Q

D

N

D

G

I

V

I

F

S

A

G

K

E

D

F

F

binding magnesium ion: D187 (= D217), E191 (= E221)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D217), E191 (= E221)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
35% identity, 57% coverage: 35:312/491 of query aligns to 14:290/327 of 8uppA

query
sites
8uppA

L

I

Q

K

G

S

K

K

V

V

V

A

I

I

V

I

G

G

C
x
F

G
|
G

A
x
S

Q
|
Q

G

G

L

H

N

A

Q

H

G

A

L

M

N

N

M

L

R

R

D

D

S

N

G

G

L

V

D

N

I

V

S

T

Y

I

A

G

L

L

R
|
R

K

-

E

-

A

-

I

-

A

-

E

E

K

G

R
x
S

A

V

S

S

W

A

R

V

K

K

A

A

T

K

E

N

N

A

G

G

F

F

K

E

V

V

G

M

T

S

Y

V

E

S

E

E

L

A

I

S

P

K

Q

I

A

A

D

D

L

V

V

I

I

M

N

I

L
|
L

T

A

P
|
P

D
|
D

K

E

Q
x
I

H
x
Q

S

A

D

D

V
x
I

V

F

R

N

T

V

-

E

V

I

Q

K

P

P

L

N

M

L

K

S

D

E

G

G

A

K

A

A

L

I

G

A

Y

F

S

A

H
|
H

G

G

F

F

N

N

I

I

V

H

E

Y

V

G

G

Q

E

I

Q

V

I

V

R

P

K

K

D

G

I

V

T

D

V

V

V

I

M

M

V

I

A

A

P

P

K

K

C
x
A

P
|
P

G

G

T

H

E

T

V

V

R

R

E

N

E

E

Y

F

K

T

R

L

G

G

F

G

G

G

V

T

P

P

T

C

L

L

I

I

A

A

V

I

H

H

P

Q

E

D

N

E

D

S

P

K

K

N

G

A

E

K

G

N

M

L

A

A

I

L

A

S

K

-

A

-

W

Y

A

A

A

S

A

A

T

I

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

A

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

M

G

L

L

Q

S

A

A

G

L

S

I

L

Q

L

A

C

G

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

E

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

L

I

H

Q
x
E

F

M

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

Q

G

G

I

I

T

A

L

D

M

M

M

R

D

Y

R

S

L
x
I

S
|
S

N

N

P

T

A
|
A

K

E

L

Y

R

G

A

D

Y

Y

-

I

-

T

-

G

-

P

-

K

A

I

L

I

S

T

E

E

Q

E

L

T

K

K

E

K

I

A

M

M

A

K

P

G

L

V

F

L

Q

K

K

D

H

I

M

Q

D

N

D

G

I

V

I

F

S

A

G

K

E

D

F

F

binding magnesium ion: D190 (= D217), E194 (= E221)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (≠ C45), G25 (= G46), S26 (≠ A47), Q27 (= Q48), R47 (= R68), S50 (≠ R76), L79 (= L105), P81 (= P107), D82 (= D108), I84 (≠ Q110), Q85 (≠ H111), I88 (≠ V114), H107 (= H132), P132 (= P157), I250 (≠ L277), S251 (= S278)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (≠ C156), P132 (= P157), D190 (= D217), E194 (= E221), E230 (≠ Q255), I250 (≠ L277), S251 (= S278), A254 (= A281)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
35% identity, 57% coverage: 35:312/491 of query aligns to 14:290/327 of 8sxdA

query
sites
8sxdA

L

I

Q

K

G

S

K

K

V

V

V

A

I

I

V

I

G
|
G

C
x
F

G
|
G

A
x
S

Q
|
Q

G

G

L

H

N

A

Q

H

G

A

L

M

N

N

M

L

R

R

D

D

S

N

G

G

L

V

D

N

I

V

S

T

Y

I

A

G

L

L

R

R

K

-

E

-

A

-

I

-

A

-

E

E

K

G

R
x
S

A

V

S
|
S

W

A

R

V

K

K

A

A

T

K

E

N

N

A

G

G

F

F

K

E

V

V

G

M

T

S

Y

V

E

S

E

E

L

A

I

S

P

K

Q

I

A

A

D

D

L

V

V

I

I

M

N

I

L
|
L

T
x
A

P
|
P

D
|
D

K

E

Q

I

H

Q

S

A

D

D

V

I

V

F

R

N

T

V

-

E

V

I

Q

K

P

P

L

N

M

L

K

S

D

E

G

G

A

K

A

A

L

I

G

A

Y

F

S

A

H

H

G

G

F

F

N

N

I

I

V

H

E

Y

V

G

G

Q

E

I

Q

V

I

V

R

P

K

K

D

G

I

V

T

D

V

V

V

I

M

M

V

I

A

A

P
|
P

K

K

C

A

P

P

G

G

T

H

E

T

V

V

R

R

E

N

E

E

Y

F

K

T

R

L

G

G

F

G

G

G

V

T

P

P

T

C

L

L

I

I

A

A

V

I

H

H

P

Q

E

D

N

E

D

S

P

K

K

N

G

A

E

K

G

N

M

L

A

A

I

L

A

S

K

-

A

-

W

Y

A

A

A

S

A

A

T

I

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

A

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

M

G

L

L

Q

S

A

A

G

L

S

I

L

Q

L

A

C

G

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

E

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

L

I

H

Q

E

F

M

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

Q

G

G

I

I

T

A

L

D

M

M

M

R

D

Y

R

S

L

I

S

S

N

N

P

T

A

A

K

E

L

Y

R

G

A

D

Y

Y

-

I

-

T

-

G

-

P

-

K

A

I

L

I

S

T

E

E

Q

E

L

T

K

K

E

K

I

A

M

M

A

K

P

G

L

V

F

L

Q

K

K

D

H

I

M

Q

D

N

D

G

I

V

I

F

S

A

G

K

E

D

F

F

binding magnesium ion: D190 (= D217), E194 (= E221)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G44), F24 (≠ C45), G25 (= G46), S26 (≠ A47), Q27 (= Q48), S50 (≠ R76), S52 (= S78), L79 (= L105), A80 (≠ T106), P81 (= P107), D82 (= D108), P129 (= P154)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D217), E194 (= E221)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
35% identity, 57% coverage: 35:312/491 of query aligns to 15:291/329 of 7rduA

query
sites
7rduA

L

I

Q

K

G

S

K

K

V

V

V

A

I

I

V

I

G
|
G

C
x
F

G
|
G

A
x
S

Q
|
Q

G

G

L

H

N

A

Q

H

G

A

L

M

N

N

M

L

R

R

D

D

S

N

G

G

L

V

D

N

I

V

S

T

Y

I

A

G

L

L

R
|
R

K

-

E

-

A

-

I

-

A

-

E

E

K

G

R
x
S

A

V

S
|
S

W

A

R

V

K

K

A

A

T

K

E

N

N

A

G

G

F

F

K

E

V

V

G

M

T

S

Y

V

E

S

E

E

L

A

I

S

P

K

Q

I

A

A

D

D

L

V

V

I

I

M

N

I

L
|
L

T
x
A

P
|
P

D
|
D

K

E

Q

I

H

Q

S

A

D

D

V
x
I

V

F

R

N

T

V

-

E

V

I

Q

K

P

P

L

N

M

L

K

S

D

E

G

G

A

K

A

A

L

I

G

A

Y

F

S
x
A

H
|
H

G

G

F

F

N

N

I

I

V

H

E

Y

V

G

G

Q

E

I

Q

V

I

V

R

P

K

K

D

G

I

V

T

D

V

V

V

I

M

M

V

I

A

A

P
|
P

K
|
K

C
x
A

P

P

G

G

T

H

E

T

V

V

R

R

E

N

E

E

Y

F

K

T

R

L

G

G

F

G

G

G

V

T

P

P

T

C

L

L

I

I

A

A

V

I

H

H

P

Q

E

D

N

E

D

S

P

K

K

N

G

A

E

K

G

N

M

L

A

A

I

L

A

S

K

-

A

-

W

Y

A

A

A

S

A

A

T

I

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

A

E

E

V

T

K

E

S

T

D

D

L

L

M

F

G

G

E

E

Q

Q

T

A

I

V

L

L

C

C

G

G

M

G

L

L

Q

S

A

A

G

L

S

I

L

Q

L

A

C

G

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

E

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

L

I

H

Q

E

F

M

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

I

K

Y

Q

Q

G

G

I

I

T

A

L

D

M

M

M

R

D

Y

R

S

L

I

S

S

N

N

P

T

A

A

K

E

L

Y

R

G

A

D

Y

Y

-

I

-

T

-

G

-

P

-

K

A

I

L

I

S

T

E

E

Q

E

L

T

K

K

E

K

I

A

M

M

A

K

P

G

L

V

F

L

Q

K

K

D

H

I

M

Q

D

N

D

G

I

V

I

F

S

A

G

K

E

D

F

F

binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G44), G26 (= G46), S27 (≠ A47), Q28 (= Q48), R48 (= R68), S51 (≠ R76), S53 (= S78), A81 (≠ T106), P82 (= P107), D83 (= D108), I89 (≠ V114), A107 (≠ S131), H108 (= H132), P130 (= P154), K131 (= K155), A132 (≠ C156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G44), F25 (≠ C45), G26 (= G46), S27 (≠ A47), Q28 (= Q48), S51 (≠ R76), S53 (= S78), L80 (= L105), P82 (= P107), D83 (= D108), I89 (≠ V114), A107 (≠ S131), H108 (= H132)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
34% identity, 63% coverage: 33:341/491 of query aligns to 12:315/328 of 4xiyA

query
sites
4xiyA

S

S

Y

I

L

I

Q

Q

G

S

K

K

V

V

V

A

I

I

V

I

G

G

C

Y

G

G

A

S

Q

Q

G

G

L

H

N

A

Q

H

G

A

L

C

N

N

M

L

R

K

D

D

S

S

G

G

L

V

D

D

I

V

S

Y

Y

V

A

G

L

L

R

R

K

A

E

G

A

S

I

-

A

-

E

-

K

-

R

-

A

A

S

S

W

V

R

A

K

K

A

A

T

E

E

A

N

H

G

G

F

L

K

T

V

V

G

K

T

S

Y

V

E

K

E

D

L

A

I

V

P

A

Q

A

A

A

D

D

L

V

V

V

I

M

N

I

L

L

T

T

P

P

D

D

K

E

Q

F

H

Q

S

G

D

R

V

L

V

Y

R

K

T

D

-

E

V

I

Q

E

P

P

L

N

M

L

K

K

D

K

G

G

A

A

A

T

L

L

G

A

Y

F

S

A

H

H

G

G

F

F

N

S

I

I

V

H

E

Y

V

N

G

Q

E

V

Q

V

I

P

R

R

K

A

D

D

I

L

T

D

V

V

V

I

M

M

V

I

A

A

P

P

K
|
K

C

A

P

P

G

G

T

H

E

T

V

V

R

R

E

S

E

E

Y

F

K

V

R

R

G

G

F

G

G

G

V

I

P

P

T

D

L

L

I

I

A

A

V

V

H

Y

P

-

E

-

N

Q

D

D

P

A

K

S

G

G

E

N

G

A

M

K

A

N

I

L

A

A

K

L

A

S

W

Y

A

A

A

C

A

G

T

V

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

D

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

-

G

-

C

-

V

-

E

M

L

L

V

Q

K

A

A

G

G

S

-

L

-

C

-

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

A

P

P

-

E

-

M

A

A

Y

Y

A

F

E
|
E

K

C

L

L

I

H

Q
x
E

F

L

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

M

K

F

Q

E

G

G

I

I

T

A

L

N

M

M

M

N

D

Y

R

S

L

I

S

S

N

N

P

N

A

A

K

E

L

Y

R

G

A

E

Y

Y

-

V

-

T

-

G

-

P

-

E

A

V

L

I

S

N

E

E

Q

Q

L

S

K

R

E

Q

I

A

M

M

A

R

P

N

L

A

F

L

Q

K

K

R

H

I

M

Q

D

D

D

-

I

-

S

-

G

G

E

E

F

Y

S

A

S

K

G

M

M

F

M

I

A

T

D

E

W

G

A

A

N

A

D

N

D

Y

K

P

K

S

L

M

L

T

T

A

W

Y

R

R

E

R

E

N

T

N

G

A

K

A

T

H

A

Q

F

I

E

E

T

V

active site: K130 (= K155), D190 (= D217), E194 (= E221)
binding fe (iii) ion: D190 (= D217), E194 (= E221)
binding magnesium ion: E226 (= E251), E230 (≠ Q255)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
34% identity, 63% coverage: 33:341/491 of query aligns to 12:315/338 of C1DFH7

query
sites
C1DFH7

S

S

Y

I

L

I

Q

Q

G

S

K

K

V

V

V

A

I

I

V

I

G

G

C

Y

G

G

A

S

Q

Q

G

G

L

H

N

A

Q

H

G

A

L

C

N

N

M

L

R

K

D

D

S

S

G

G

L

V

D

D

I

V

S

Y

Y

V

A

G

L

L

R

R

K

A

E

G

A

S

I

-

A

-

E

-

K

-

R

-

A

A

S

S

W

V

R

A

K

K

A

A

T

E

E

A

N

H

G

G

F

L

K

T

V

V

G

K

T

S

Y

V

E

K

E

D

L

A

I

V

P

A

Q

A

A

A

D

D

L

V

V

V

I

M

N

I

L

L

T

T

P

P

D

D

K

E

Q

F

H

Q

S

G

D

R

V

L

V

Y

R

K

T

D

-

E

V

I

Q

E

P

P

L

N

M

L

K

K

D

K

G

G

A

A

A

T

L

L

G

A

Y

F

S

A

H

H

G

G

F

F

N

S

I

I

V

H

E

Y

V

N

G

Q

E

V

Q

V

I

P

R

R

K

A

D

D

I

L

T

D

V

V

V

I

M

M

V

I

A

A

P

P

K

K

C

A

P

P

G

G

T

H

E

T

V

V

R

R

E

S

E

E

Y

F

K

V

R

R

G

G

F

G

G

G

V

I

P

P

T

D

L

L

I

I

A

A

V

V

H

Y

P

-

E

-

N

Q

D

D

P

A

K

S

G

G

E

N

G

A

M

K

A

N

I

L

A

A

K

L

A

S

W

Y

A

A

A

C

A

G

T

V

G

G

H

G

R

R

A

T

G

G

V

I

L

I

E

E

S

T

F

F

V

K

A

D

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E

E

Q

Q

T

A

I

V

L

L

C

C

G

G

-

G

-

C

-

V

-

E

M

L

L

V

Q

K

A

A

G

G

S

-

L

-

C

-

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

A

P

P

-

E

-

M

A

A

Y

Y

A

F

E
|
E

K

C

L

L

I

H

Q
x
E

F

L

G

-

W

-

E

K

T

L

I

I

T

V

E

D

A

L

L

M

K

F

Q

E

G

G

I

I

T

A

L

N

M

M

M

N

D

Y

R

S

L

I

S

S

N

N

P

N

A

A

K

E

L

Y

R

G

A

E

Y

Y

-

V

-

T

-

G

-

P

-

E

A

V

L

I

S

N

E

E

Q

Q

L

S

K

R

E

Q

I

A

M

M

A

R

P

N

L

A

F

L

Q

K

K

R

H

I

M

Q

D

D

D

-

I

-

S

-

G

G

E

E

F

Y

S

A

S

K

G

M

M

F

M

I

A

T

D

E

W

G

A

A

N

A

D

N

D

Y

K

P

K

S

L

M

L

T

T

A

W

Y

R

R

E

R

E

N

T

N

G

A

K

A

T

H

A

Q

F

I

E

E

T

V

D190 (= D217) binding Mg(2+)
E226 (= E251) binding Mg(2+)
E230 (≠ Q255) binding Mg(2+)

7q03A Ketol-acid reductoisomerase from methanothermococcus thermolithotrophicus in the close state with NADP and mg2+ (see paper)
37% identity, 58% coverage: 35:321/491 of query aligns to 14:291/328 of 7q03A

query
sites
7q03A

L

V

Q

K

G

D

K

K

K

T

V

I

V

A

I

V

V

I

G

G

C
x
Y

G
|
G

A
x
S

Q
|
Q

G

G

L

R

N

A

Q

Q

G

S

L

L

N

N

M

M

R

K

D

D

S

S

G

G

L

L

D

K

I

V

S

V

Y

V

A

G

L

L

R
|
R

K

P

E

N

A

G

I

-

A

-

E

-

K

-

R

-

A

A

S
|
S

W

W

R

N

K

K

A

A

T

K

E

E

N

D

G

G

F

H

K

E

V

V

G

L

T

S

Y

I

E

E

L

A

I

A

P

E

Q

K

A

A

D

D

L

I

V

I

I

H

N

I

L
|
L

T
x
I

P
|
P

D
|
D

K

E

Q
x
I

H

Q

S

G

D

D

V

V

V

Y

-

N

R

K

T

Q

V

I

Q

K

P

P

L

Y

M

L

K

K

D

E

G

G

A

K

A

T

L

L

G

S

Y

F

S

S

H
|
H

G

G

F

Y

N

N

I

I

V

-

E

H

V

Y

G

G

E

Y

Q

I

I

V

R

P

K

P

D

E

-

G

I

V

T

N

V

V

M

M

V

V

A

A

P

P

K

K

C

S

P
|
P

G

G

T

A

E

M

V

V

R

R

E

R

E

T

Y

Y

K

E

R

E

G

G

F

F

G

G

V

V

P

P

T

G

L

L

I

V

A

C

V

V

H

-

P

-

E

E

N

K

D

D

P

A

K

T

G

G

E

D

G

A

M

L

A

D

I

I

A

A

K

L

A

G

W

M

A

A

A

K

A

G

T

V

G

G

G

L

H

T

R

R

A

A

G

G

V

V

L

I

E

Q

S

T

F

F

V

R

A

E

E

E

V

T

K

E

S

T

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

A

I

V

L

L

C

C

G

G

-

G

-

V

-

T

-

E

M

L

L

I

Q

K

A

A

G

G

S

-

L

-

C

-

F

F

D

E

K

T

L

L

V

V

E

E

E

A

G

G

T

Y

D

S

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

T

L

C

I

H

Q

E

F

L

G

-

W

-

E

K

T

L

I

I

T

I

E

D

A

L

L

I

K

Y

Q

Q

G

K

G

G

I

F

T

A

L

G

M

M

M

W

D

N

R

D

L

V

S

S

N

N

P

T

A

A

K

E

L

Y

-

G

-

L

-

T

-

R

R

R

A

S

Y

R

A

V

L

I

S

N

E

E

Q

Q

L

S

K

R

E

Q

I

E

M

M

A

-

P

-

L

-

F

-

Q

R

K

K

H

I

M

L

D

K

D

E

I

I

I

Q

S

D

G

G

E

R

F

F

S

T

S

K

G

-

M

-

A

-

D

E

W

W

A

A

binding magnesium ion: D190 (= D217), E194 (= E221)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y24 (≠ C45), G25 (= G46), S26 (≠ A47), Q27 (= Q48), R47 (= R68), S52 (= S78), L79 (= L105), I80 (≠ T106), P81 (= P107), D82 (= D108), I84 (≠ Q110), H107 (= H132), P132 (= P157)

4xdyA Structure of nadh-preferring ketol-acid reductoisomerase from an uncultured archean (see paper)
35% identity, 65% coverage: 25:341/491 of query aligns to 5:320/331 of 4xdyA

query
sites
4xdyA

R

H

D

D

E

E

F

D

A

V

D

D

G

D

A

-

S

S

Y

I

L

L

Q

R

G

D

K

K

K

T

V

I

V

A

I

V

V

M

G

G

C

Y

G
|
G

A
|
A

Q
|
Q

G

G

L

D

N

A

Q

Q

G

A

L

N

N

C

M

L

R

K

D

D

S

S

G

G

L

I

D

N

I

V

S

V

Y

I

A

G

L
x
E

R

T

K

E

E

I

A
x
L

I

G

A

G

E

N

K

K

R
x
N

A

P

S
|
S

W

W

R

E

K

K

A

A

T

K

E

E

N

D

G

G

F

F

K

E

V

V

G

L

T

P

Y

I

E

D

E

K

L

A

I

A

P

E

Q

K

A

G

D

D

L

V

V

V

I

H

N

I

L
|
L

T
x
L

P
|
P

D
|
D

K

E

Q

V

H

Q

S

P

D

A

V
x
I

V

Y

R

E

T

N

-

Q

V

I

Q

K

P

P

L

Q

M

L

K

K

D

A

G

G

A

K

A

A

L

L

G

C

Y

F

S

S

H
|
H

G

G

F

F

N

N

I

I

V

C

E

F

V

K

G

R

E

I

Q

V

I

P

R

P

K

E

D

D

I

V

T

D

V

V

V

I

M

M

V

V

A

A

P

P

K
|
K

C

A

P
|
P

G

G

T

T

E

E

V

E

R

R

E

K

E

A

Y

Y

K

L

R

E

G

G

F

F

G

G

V

V

P

P

T

G

L

L

I

V

A

A

V

V

H

-

P

-

E

K

N

Q

D

N

P

P

K

S

G

G

E

E

G

A

M

R

A

E

I

V

A

A

K

L

A

A

W

M

A

T

A

K

A

A

T

M

G

H

G

W

H

T

R

K

A

A

G

G

V

I

L

L

E

E

S

C

S

T

F

F

V

E

A

Q

E

E

V

T

K

Y

S

E

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

C

I

V

L

L

C

C

G

G

M

G

L

L

Q

V

A

E

G

L

S

M

L

R

L

N

C

G

F

F

D

E

K

V

L

L

V

V

E

E

E

A

G

G

T

Y

D

P

P

P

-

E

-

M

A

A

Y

Y

A

F

E

E

K

C

L

V

I

H

Q
x
E

F

M

G

-

W

-

E

K

T

L

I
|
I

T

V

E

D

A

L

L

V

K

W

Q

Q

G

G

I

I

T

K

L

R

M

M

M

A

D

E

R

V

L
x
I

S
|
S

N

N

P

T

A
|
A

K

E

L

Y

R

G

A

M

Y

W

A

A

L

V

S

G

E

H

Q

Q

L

-

K

-

E

-

I

I

M

I

A

G

P

P

L

E

F

V

Q

K

K

E

H

K

M

M

D

K

D

E

I

A

I

L

-

K

-

R

-

V

-

E

S

N

G

G

E

E

F

F

S

A

S

N

G

-

M

-

A

-

D

E

W

W

A

V

N

D

D

E

D

Y

K

K

K

R

-

G

-

I

-

P

-

F

L

L

L

K

T

A

W

S

R

R

E

E

E

K

T

M

G

G

K

E

T

H

A

Q

F

V

E

E

T

T

active site: K135 (= K155), D195 (= D217), E199 (= E221)
binding n-hydroxy-n-isopropyloxamic acid: P137 (= P157), D195 (= D217), E199 (= E221), E235 (≠ Q255), I239 (= I261), I255 (≠ L277), S256 (= S278), A259 (= A281)
binding magnesium ion: D195 (= D217), E199 (= E221)
binding 1,4-dihydronicotinamide adenine dinucleotide: G25 (= G46), A26 (= A47), Q27 (= Q48), E46 (≠ L67), L50 (≠ A71), N55 (≠ R76), S57 (= S78), L84 (= L105), L85 (≠ T106), P86 (= P107), D87 (= D108), I93 (≠ V114), H112 (= H132), P137 (= P157)

Q64BR7 Ketol-acid reductoisomerase (NAD(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.382 from Uncultured archaeon GZfos26G2 (see paper)
35% identity, 65% coverage: 25:341/491 of query aligns to 5:320/332 of Q64BR7

query
sites
Q64BR7

R

H

D

D

E

E

F

D

A

V

D

D

G

D

A

-

S

S

Y

I

L

L

Q

R

G

D

K

K

K

T

V

I

V

A

I

V

V

M

G

G

C
x
Y

G
|
G

A
|
A

Q
|
Q

G

G

L

D

N

A

Q

Q

G

A

L

N

N

C

M

L

R

K

D

D

S

S

G

G

L

I

D

N

I

V

S

V

Y

I

A

G

L
x
E

R

T

K

E

E

I

A

L

I

G

A

G

E

N

K

K

R
x
N

A

P

S
|
S

W

W

R

E

K

K

A

A

T

K

E

E

N

D

G

G

F

F

K

E

V

V

G

L

T

P

Y

I

E

D

E

K

L

A

I

A

P

E

Q

K

A

G

D

D

L

V

V

V

I

H

N

I

L

L

T

L

P

P

D
|
D

K
x
E

Q
x
V

H
x
Q

S

P

D

A

V

I

V

Y

R

E

T

N

-

Q

V

I

Q

K

P

P

L

Q

M

L

K

K

D

A

G

G

A

K

A

A

L

L

G

C

Y

F

S

S

H

H

G

G

F

F

N

N

I

I

V

C

E

F

V

K

G

R

E

I

Q

V

I

P

R

P

K

E

D

D

I

V

T

D

V

V

V

I

M

M

V

V

A

A

P

P

K

K

C

A

P

P

G
|
G

T

T

E

E

V

E

R

R

E

K

E

A

Y

Y

K

L

R

E

G

G

F

F

G

G

V

V

P

P

T

G

L

L

I

V

A

A

V

V

H

-

P

-

E

K

N

Q

D

N

P

P

K

S

G

G

E

E

G

A

M

R

A

E

I

V

A

A

K

L

A

A

W

M

A

T

A

K

A

A

T

M

G

H

G

W

H

T

R

K

A

A

G

G

V

I

L

L

E

E

S

C

S

T

F

F

V

E

A

Q

E

E

V

T

K

Y

S

E

D
|
D

L

L

M

F

G

G

E
|
E

Q

Q

T

C

I

V

L

L

C

C