SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 18220 FitnessBrowser__Keio:18220 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
34% identity, 91% coverage: 8:147/154 of query aligns to 6:141/144 of 1j6tA

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binding phosphite ion: R46 (≠ V51), H62 (= H68), H108 (= H113)

P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 91% coverage: 8:147/154 of query aligns to 498:633/637 of P00550

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R538 (≠ V51) Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA
H554 (= H68) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

384 modified: Phosphocysteine; by EIIA

Query Sequence

>18220 FitnessBrowser__Keio:18220
MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
APGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQ
IVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory