SitesBLAST
Comparing 1937180 b3903 L-rhamnose isomerase (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 9 hits to proteins with known functional sites (download)
P32170 L-rhamnose isomerase; RhamIso; EC 5.3.1.14 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:419/419 of query aligns to 1:419/419 of P32170
- H95 (= H95) binding
- E226 (= E226) binding
- ESK 226:228 (= ESK 226:228) binding
- D259 (= D259) binding
- H262 (= H262) binding ; binding
- H286 (= H286) binding
- D294 (= D294) binding
- D296 (= D296) binding
- D326 (= D326) binding ; binding
1de5A L-rhamnose isomerase (see paper)
100% identity, 100% coverage: 2:418/419 of query aligns to 1:417/417 of 1de5A
- active site: W184 (= W185), E225 (= E226), K227 (= K228), D258 (= D259), H261 (= H262), H285 (= H286), D293 (= D294), D295 (= D296), D325 (= D326)
- binding l-rhamnitol: W39 (= W40), I58 (= I59), H94 (= H95), W184 (= W185), E225 (= E226), K227 (= K228), H261 (= H262), H285 (= H286), D293 (= D294), D325 (= D326)
- binding zinc ion: E225 (= E226), D258 (= D259), H285 (= H286), D325 (= D326)
1de6A L-rhamnose isomerase (see paper)
100% identity, 99% coverage: 3:418/419 of query aligns to 1:416/416 of 1de6A
- active site: W183 (= W185), E224 (= E226), K226 (= K228), D257 (= D259), H260 (= H262), H284 (= H286), D292 (= D294), D294 (= D296), D324 (= D326)
- binding manganese (ii) ion: H260 (= H262), D292 (= D294), D294 (= D296)
- binding l-rhamnose: I57 (= I59), W183 (= W185), E224 (= E226), K226 (= K228), H260 (= H262), D324 (= D326), F326 (= F328)
- binding zinc ion: E224 (= E226), D257 (= D259), H284 (= H286), D324 (= D326)
3uvaC Crystal structure of l-rhamnose isomerase mutant w38f from bacillus halodurans in complex with mn (see paper)
57% identity, 99% coverage: 5:418/419 of query aligns to 2:405/405 of 3uvaC
- active site: W172 (= W185), E213 (= E226), K215 (= K228), D246 (= D259), H249 (= H262), H273 (= H286), D281 (= D294), D283 (= D296), D313 (= D326)
- binding manganese (ii) ion: E213 (= E226), D246 (= D259), D246 (= D259), H249 (= H262), H249 (= H262), H273 (= H286), D281 (= D294), D283 (= D296), D313 (= D326)
8jq5B Crystal structure of lactobacillus rhamnosus l-rhamnose isomerase in complex with d-allulose (see paper)
51% identity, 99% coverage: 4:418/419 of query aligns to 6:420/421 of 8jq5B
- binding manganese (ii) ion: E228 (= E226), D261 (= D259), D261 (= D259), H264 (= H262), H288 (= H286), D296 (= D294), D298 (= D296), D328 (= D326)
- binding D-psicose: I61 (= I59), H97 (= H95), W187 (= W185), E228 (= E226), K230 (= K228), H264 (= H262), D328 (= D326), F330 (= F328)
8jq5A Crystal structure of lactobacillus rhamnosus l-rhamnose isomerase in complex with d-allulose (see paper)
51% identity, 99% coverage: 4:418/419 of query aligns to 6:420/420 of 8jq5A
- binding manganese (ii) ion: E228 (= E226), D261 (= D259), D261 (= D259), H264 (= H262), H288 (= H286), D296 (= D294), D298 (= D296), D328 (= D326)
- binding D-psicose: W42 (= W40), I61 (= I59), H97 (= H95), W187 (= W185), E228 (= E226), K230 (= K228), H264 (= H262), D328 (= D326), F330 (= F328)
- binding alpha-D-psicofuranose: D153 (= D151), K155 (≠ S153)
8jq6C Crystal structure of lactobacillus rhamnosus l-rhamnose isomerase in complex with d-allose (see paper)
50% identity, 99% coverage: 4:418/419 of query aligns to 6:409/409 of 8jq6C
- binding alpha-D-allopyranose: H86 (= H95), W176 (= W185), E217 (= E226), K219 (= K228), H253 (= H262), D317 (= D326)
- binding manganese (ii) ion: E217 (= E226), D250 (= D259), H253 (= H262), H277 (= H286), D285 (= D294), D287 (= D296), D317 (= D326)
8jq6A Crystal structure of lactobacillus rhamnosus l-rhamnose isomerase in complex with d-allose (see paper)
50% identity, 99% coverage: 4:418/419 of query aligns to 6:409/409 of 8jq6A
- binding beta-D-allopyranose: W42 (= W40), H86 (= H95), W176 (= W185), E217 (= E226), K219 (= K228), H253 (= H262), D317 (= D326), D393 (≠ E402), M395 (≠ L404), N396 (≠ E405)
- binding manganese (ii) ion: E217 (= E226), D250 (= D259), H253 (= H262), H277 (= H286), D285 (= D294), D287 (= D296), D317 (= D326)
8jq4A Crystal structure of lactobacillus rhamnosus l-rhamnose isomerase in complex with l-rhamnose (see paper)
49% identity, 99% coverage: 4:418/419 of query aligns to 6:408/408 of 8jq4A
- binding manganese (ii) ion: E216 (= E226), D249 (= D259), D249 (= D259), H252 (= H262), H276 (= H286), D284 (= D294), D286 (= D296), D316 (= D326)
- binding alpha-L-rhamnopyranose: D106 (≠ K116), D110 (≠ E120), K165 (≠ Q175)
- binding beta-L-rhamnopyranose: H85 (= H95), W175 (= W185), E216 (= E226), H252 (= H262), D316 (= D326)
Query Sequence
>1937180 b3903 L-rhamnose isomerase (NCBI)
MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDDVSGFENPEGSLTGGIQ
ATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESDTPVSRDQIKPEHFKNWVE
WAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWIDHCKASRRVSAYFGEQLGTPS
VMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNPAHHIDAVESKLFGIGAESYTVG
SNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAAMLYVPQLLLHVSRPVRWDSDHVVL
LDDETQAIASEIVRHDLFDRVHIGLDFFDASINRIAAWVIGTRNMKKALLRALLEPTAEL
RKLEAAGDYTARLALLEEQKSLPWQAVWEMYCQRHDTPAGSEWLESVRAYEKEILSRRG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory