SitesBLAST
Comparing 201857 FitnessBrowser__MR1:201857 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3occF Crystal structure of pnp with dadmeimmh from yersinia pseudotuberculosis
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/238 of 3occF
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 7-[[(3R,4R)-3-(hydroxymethyl)-4-oxidanyl-pyrrolidin-1-ium-1-yl]methyl]-3,5-dihydropyrrolo[3,2-d]pyrimidin-4-one: H4 (= H4), R43 (= R43), M64 (= M64), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204), I206 (≠ C206)
5iu6A Crystal structure of e.Coli purine nucleoside phosphorylase with 7- deazahypoxanthine (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 5iu6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 7H-pyrrolo[2,3-d]pyrimidin-4-ol: C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179)
5i3cA Crystal structure of e.Coli purine nucleoside phosphorylase with acycloguanosine (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 5i3cA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 9-hyroxyethoxymethylguanine: S90 (= S90), C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), I206 (≠ C206)
- binding sulfate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
3ut6A Crystal structure of e. Coli pnp complexed with po4 and formycin a (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 3ut6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding (1S)-1-(7-amino-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro-D-ribitol: M64 (= M64), S90 (= S90), C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R24 (= R24), R87 (= R87), G89 (= G89), S90 (= S90)
1pw7A Crystal structure of e. Coli purine nucleoside phosphorylase complexed with 9-beta-d-arabinofuranosyladenine and sulfate/phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pw7A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding phosphate ion: G20 (= G20), R87 (= R87), S90 (= S90)
- binding 2-(6-amino-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: S90 (= S90), C91 (≠ L91), F159 (≠ Y159), E179 (≠ D179), E181 (= E181), S203 (= S203)
1pr0A Escherichia coli purine nucleoside phosphorylase complexed with inosine and phosphate/sulfate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pr0A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding inosine: R87 (= R87), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
1pkeA Crystal structure of e. Coli purine nucleoside phosphorylase complexed with 2-fluoro-2'-deoxyadenosine and sulfate/phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pkeA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206)
- binding 5-(6-amino-2-fluoro-purin-9-yl)-2-hydroxymethyl-tetrahydro-furan-3-ol: S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
1pk9A Crystal structure of e. Coli purine nucleoside phosphorylase complexed with 2-fluoroadenosine and sulfate/phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pk9A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 2-(6-amino-2-fluoro-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: M64 (= M64), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R87 (= R87), S90 (= S90)
1pk7A Crystal structure of e. Coli purine nucleoside phosphorylase complexed with adenosine and sulfate/phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1pk7A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding adenosine: M64 (= M64), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), M180 (= M180), E181 (= E181), D204 (= D204)
- binding phosphate ion: G20 (= G20), R87 (= R87)
1otyA Native pnp +allo (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1otyA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 6-methylpurine: G92 (= G92), F159 (≠ Y159), V178 (≠ I178), M180 (= M180)
1k9sD Purine nucleoside phosphorylase from e. Coli in complex with formycin a derivative and phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1k9sD
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 2-hydroxymethyl-5-(7-methylamino-3h-pyrazolo[4,3-d]pyrimidin-3-yl)-tetrahydro-furan-3,4-diol: M64 (= M64), S90 (= S90), C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204), I206 (≠ C206)
- binding 2-(7-amino-6-methyl-3h-pyrazolo[4,3-d]pyrimidin-3-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: H4 (= H4), R43 (= R43)
- binding phosphate ion: G20 (= G20), R24 (= R24), R87 (= R87), G89 (= G89), S90 (= S90)
1k9sA Purine nucleoside phosphorylase from e. Coli in complex with formycin a derivative and phosphate (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1k9sA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 2-(7-amino-6-methyl-3h-pyrazolo[4,3-d]pyrimidin-3-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: M64 (= M64), S90 (= S90), C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), S203 (= S203)
- binding phosphate ion: G20 (= G20), R24 (= R24), R87 (= R87), G89 (= G89), S90 (= S90)
1a69A Purine nucleoside phosphorylase in complex with formycin b and sulphate (phosphate) (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1a69A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding formycin b: M64 (= M64), S90 (= S90), C91 (≠ L91), G92 (= G92), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181), D204 (= D204)
P0ABP8 Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1 from Escherichia coli (strain K12) (see 5 papers)
55% identity, 99% coverage: 2:233/234 of query aligns to 3:234/239 of P0ABP8
- G21 (= G20) binding in other chain
- R25 (= R24) binding in other chain; mutation to A: Severe loss of catalytic activity. 20-fold decrease in affinity for phosphate.
- K27 (= K26) modified: N6-acetyllysine
- R44 (= R43) binding
- RVGS 88:91 (≠ RIGS 87:90) binding in other chain
- D205 (= D204) active site, Proton donor; mutation D->A,N: Severe loss of catalytic activity.
- R218 (= R217) Important for catalytic activity; mutation to A: Severe loss of catalytic activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P0ABP9 Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1 from Escherichia coli O157:H7 (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 3:234/239 of P0ABP9
- H5 (= H4) binding
- G21 (= G20) binding in other chain
- R25 (= R24) binding in other chain
- R44 (= R43) binding
- RVGS 88:91 (≠ RIGS 87:90) binding in other chain
- EME 180:182 (≠ DME 179:181) binding in other chain
- SD 204:205 (= SD 203:204) binding in other chain
1ovgA M64v pnp +mepdr (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1ovgA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 9-(2-deoxy-beta-d-ribofuranosyl)-6-methylpurine: S90 (= S90), F159 (≠ Y159), V178 (≠ I178)
1ov6A M64v pnp + allo (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1ov6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 9-(6-deoxy-beta-d-allofuranosyl)-6-methylpurine: S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), S203 (= S203)
1oumA M64v pnp +talo (see paper)
55% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1oumA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding 9-(6-deoxy-alpha-l-talofuranosyl)-6-methylpurine: D21 (= D21), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), M180 (= M180), E181 (= E181)
1vhwA Crystal structure of purine nucleoside phosphorylase with adenosine (see paper)
53% identity, 99% coverage: 2:233/234 of query aligns to 2:233/237 of 1vhwA
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206), R217 (= R217)
- binding adenosine: H4 (= H4), R43 (= R43), M64 (= M64), R87 (= R87), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), M180 (= M180), E181 (= E181)
1pr6A Escherichia coli purine nucleoside phosphorylase complexed with 9- beta-d-xylofuranosyladenine and phosphate/sulfate (see paper)
54% identity, 99% coverage: 2:233/234 of query aligns to 2:230/234 of 1pr6A
- active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (= D204), I206 (≠ C206)
- binding phosphate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)
- binding 2-(6-amino-octahydro-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: M64 (= M64), S90 (= S90), C91 (≠ L91), F159 (≠ Y159), V178 (≠ I178), E179 (≠ D179), M180 (= M180), E181 (= E181)
Query Sequence
>201857 FitnessBrowser__MR1:201857
MTAHINAQPTDFAETVIMPGDPLRAKYIAETYLTDAVEVTNVRNMLGYTGYYQGQRISVM
GHGMGISSMVLYGHELINFFGVKRIIRIGSLGATQQHVEMRDVILAQAAGTDSPTNAKRS
SGYHMATSATFSLLHKAYTKANEKGISVKVGNVFSGDLYYDPDEDMIPALERFGVLGIDM
EVAGLYGLAHQQGIESLAILTVSDHCLTGEETTAQERQLSFNNMIELALETALN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory