SitesBLAST

Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
35% identity, 80% coverage: 53:260/261 of query aligns to 2:225/234 of Q7WTT9

query
sites
Q7WTT9

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C12 (≠ F63) binding [4Fe-4S] cluster
C15 (≠ P66) binding [4Fe-4S] cluster
C18 (≠ Y69) binding [4Fe-4S] cluster
C22 (= C76) binding [4Fe-4S] cluster
C57 (= C124) binding [4Fe-4S] cluster
C60 (= C127) binding [4Fe-4S] cluster
C65 (= C132) binding [4Fe-4S] cluster
C69 (= C136) binding [4Fe-4S] cluster
C89 (= C156) binding [4Fe-4S] cluster
C92 (= C159) binding [4Fe-4S] cluster
C95 (= C162) binding [4Fe-4S] cluster
C99 (= C166) binding [4Fe-4S] cluster
C164 (= C200) binding [4Fe-4S] cluster
C167 (= C203) binding [4Fe-4S] cluster
C179 (= C215) binding [4Fe-4S] cluster
C183 (≠ S219) binding [4Fe-4S] cluster

2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
46% identity, 52% coverage: 124:260/261 of query aligns to 58:178/193 of 2vpyF

query
sites
2vpyF

C
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binding pentachlorophenol: I91 (= I157), C93 (= C159)
binding iron/sulfur cluster: C58 (= C124), L59 (≠ N125), C61 (= C127), P65 (= P131), C66 (= C132), C70 (= C136), P71 (= P137), V83 (= V149), C90 (= C156), I91 (= I157), A92 (≠ G158), C93 (= C159), G94 (≠ R160), C96 (= C162), C100 (= C166), P101 (= P167), Y102 (= Y168), R105 (= R171), V113 (= V197), C116 (= C200), F118 (= F202), C119 (= C203), P129 (= P213), A130 (= A214), C131 (= C215), C135 (≠ S219), C139 (≠ I223), R140 (vs. gap)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 15, 16, 19, 23, 27, 36, 38, 55, 57, 189, 190

2vpwF Polysulfide reductase with bound menaquinone (see paper)
46% identity, 52% coverage: 124:260/261 of query aligns to 58:178/193 of 2vpwF

query
sites
2vpwF

C
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H

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binding iron/sulfur cluster: C58 (= C124), L59 (≠ N125), H60 (= H126), C61 (= C127), P65 (= P131), C66 (= C132), C70 (= C136), P71 (= P137), V83 (= V149), C90 (= C156), I91 (= I157), A92 (≠ G158), C93 (= C159), G94 (≠ R160), C96 (= C162), C100 (= C166), P101 (= P167), Y102 (= Y168), R105 (= R171), V113 (= V197), C116 (= C200), F118 (= F202), C119 (= C203), P129 (= P213), A130 (= A214), C131 (= C215), C135 (≠ S219), C139 (≠ I223), R140 (vs. gap)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 15, 16, 19, 23, 27, 36, 38, 55, 57, 189, 190

6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
30% identity, 86% coverage: 37:260/261 of query aligns to 671:917/929 of 6lodB

query
sites
6lodB

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binding fe3-s4 cluster: V758 (= V135), C759 (= C136), P760 (= P137), C779 (= C156), V780 (≠ I157), G781 (= G158), T782 (≠ C159), C785 (= C162), M838 (≠ V197)
binding heme c: N777 (≠ H154), R778 (= R155), R852 (≠ Q211), I853 (≠ L212), R856 (≠ C215)
binding iron/sulfur cluster: C692 (≠ F63), N693 (≠ T64), S694 (= S65), C695 (≠ Y69), N696 (≠ S70), A697 (≠ R71), C698 (≠ V72), C702 (= C76), N706 (≠ H80), W724 (= W93), I727 (≠ T96), L746 (= L123), C747 (= C124), Q748 (≠ N125), Q749 (≠ H126), C750 (= C127), P754 (= P131), C755 (= C132), N772 (≠ V149), C789 (= C166), Y791 (= Y168), V793 (≠ A170), R794 (= R171), K840 (= K199), C841 (= C200), F843 (= F202), C844 (= C203), T869 (vs. gap), C871 (vs. gap), C875 (vs. gap), I880 (= I223)

6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
30% identity, 72% coverage: 75:261/261 of query aligns to 702:944/948 of 6btmB

query
sites
6btmB

A

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binding fe3-s4 cluster: C789 (= C136), C809 (= C156), V810 (≠ I157), T812 (≠ C159), C815 (= C162), M862 (≠ V197)
binding iron/sulfur cluster: C777 (= C124), Q778 (≠ N125), C780 (= C127), C785 (= C132), N802 (≠ V149), C819 (= C166), R824 (= R171)

Sites not aligning to the query:

binding (2R)-3-{[(2R)-2-(decanoylamino)-3-hydroxypropyl]sulfanyl}propane-1,2-diyl ditetradecanoate: 1, 2

8k9fB Cryo-em structure of the photosynthetic alternative complex iii from chloroflexus aurantiacus at 2.9 angstrom (see paper)
30% identity, 72% coverage: 73:260/261 of query aligns to 726:939/951 of 8k9fB

query
sites
8k9fB

I

V

E

V

A

A

C
|
C

H

Q

S

A

I

E

H
x
N

N

N

V

I

P

A

S

V

I

V

E

G

G

K

N

D

Q

Q

-

V

-

S

-

R

-

G

-

R

E

E

I

M

K

H

W
|
W

L
x
I

W

R

T

I

D

D

T

R

Y

Y

E

F

A

A

T

G

F

-

P

E

D

D

D

L

M

D

N

N

P

P

H

S

L

I

S

-

Q

-

E

-

V

-

L

-

E

-

R

-

Y

Y

L

M

L
x
P

-

V

-

N

C
|
C

N
x
M

H
x
Q

C
|
C

E

E

N

K

P

A

P

P

C
|
C

V

E

R

V

V

V

C
|
C

P
|
P

T

V

K

A

A

A

T

T

F

V

K

H

R

D

A

Y

D

E

G

G

I

L

V
x
N

V

N

M
|
M

D

V

Y

Y

H
x
N

R
|
R

C
|
C

I

V

G
|
G

C
x
T

R
x
K

F
x
Y

C
|
C

M

S

A

N

G

N

C
|
C

P

P

Y
|
Y

G

K

A
x
V

R

R

S

R

F

F

N

N

F

F

-

L

-

Q

-

Y

-

S

-

D

G

T

D

T

P

T

Q

E

P

T

H

F

I

K

K

L

A

A

I

F

N

N

P

P

K

D

F

V

P

T

A

V

R

R

T

I

R

R

G

G

V

V

V

M

E

E

K

K

C
|
C

T

T

F
x
Y

C
|
C

S

V

E

Q

R

R

L

I

A

S

V

G

G

A

Q
x
R

L

I

P

A

-

A

-
x
K

-

R

-

A

-

V

-

Q

-

A

-

G

-

Q

-

S

-

Y

-

V

-

I

-

S

-

D

-

G

-

A

-

I

-

Q

-
x
T

A

A

C
|
C

V

E

E

Q

A

A

-
x
C

S
x
P

N
x
T

G

G

A

A

I

I

L

V

F

F

G

G

D

D

L

I

D

N

D

D

P

S

N

N

S

S

P

R

V

V

R

A

K

K

A

W

L

K

N

A

E

E

N

G

Y

H

S

N

I

Y

R

G

R

L

K

L

P

G

S

F

V

L

G

N

T

T

Q

V

P

P

G

R

V

T

Y

T

Y

Y

I

L

binding fe3-s4 cluster: C787 (= C136), P788 (= P137), M802 (= M151), C807 (= C156), G809 (= G158), T810 (≠ C159), K811 (≠ R160), Y812 (≠ F161), C813 (= C162)
binding heme c: N805 (≠ H154), R806 (= R155), R867 (≠ Q211), K871 (vs. gap)
binding iron/sulfur cluster: C729 (= C76), N733 (≠ H80), W751 (= W93), I752 (≠ L94), P772 (≠ L123), C775 (= C124), M776 (≠ N125), Q777 (≠ H126), C778 (= C127), C783 (= C132), N800 (≠ V149), C817 (= C166), Y819 (= Y168), V821 (≠ A170), C856 (= C200), Y858 (≠ F202), C859 (= C203), T891 (vs. gap), C893 (= C215), C897 (vs. gap), P898 (≠ S219), T899 (≠ N220)

Sites not aligning to the query:

binding heme c: 3
binding magnesium ion: 4, 7
binding iron/sulfur cluster: 719, 721, 722, 723, 725

8k9eB Cryo-em structure of the photosynthetic alternative complex iii from chloroflexus aurantiacus at 3.3 angstrom (see paper)
30% identity, 72% coverage: 73:260/261 of query aligns to 726:939/951 of 8k9eB

query
sites
8k9eB

I

V

E

V

A

A

C
|
C

H

Q

S

A

I

E

H
x
N

N

N

V

I

P

A

S

V

I

V

E

G

G

K

N

D

Q

Q

-

V

-

S

-

R

-

G

-

R

E

E

I

M

K

H

W

W

L

I

W

R

T
x
I

D

D

T

R

Y

Y

E

F

A

A

T

G

F

-

P

E

D

D

D

L

M

D

N

N

P

P

H

S

L

I

S

-

Q

-

E

-

V

-

L

-

E

-

R

-

Y

Y

L

M

L

P

-

V

-

N

C
|
C

N
x
M

H

Q

C
|
C

E

E

N

K

P

A

P
|
P

C
|
C

V

E

R

V

V

V

C
|
C

P

P

T

V

K

A

A

A

T

T

F

V

K

H

R

D

A

Y

D

E

G

G

I

L

V
x
N

V

N

M
|
M

D

V

Y

Y

H
x
N

R

R

C
|
C

I
x
V

G
|
G

C
x
T

R
x
K

F
x
Y

C
|
C

M

S

A

N

G

N

C
|
C

P

P

Y
|
Y

G

K

A
x
V

R
|
R

S

R

F

F

N

N

F

F

-

L

-

Q

-

Y

-

S

-

D

G

T

D

T

P

T

Q

E

P

T

H

F

I

K

K

L

A

A

I

F

N

N

P

P

K

D

F

V

P

T

A

V

R

R

T

I

R

R

G

G

V

V

V
x
M

E

E

K

K

C
|
C

T

T

F
x
Y

C
|
C

S

V

E

Q

R

R

L

I

A

S

V

G

G

A

Q
x
R

L

I

P

A

-

A

-
x
K

-

R

-

A

-

V

-

Q

-

A

-

G

-

Q

-

S

-

Y

-

V

-

I

-

S

-

D

-

G

-

A

-

I

-

Q

-
x
T

A

A

C
|
C

V

E

E

Q

A

A

-
x
C

S

P

N
x
T

G

G

A

A

I

I

L

V

F

F

G

G

D

D

L

I

D

N

D

D

P

S

N

N

S

S

P

R

V

V

R

A

K

K

A

W

L

K

N

A

E

E

N

G

Y

H

S

N

I

Y

R

G

R

L

K

L

P

G

S

F

V

L

G

N

T

T

Q

V

P

P

G

R

V

T

Y

T

Y

Y

I

L

binding fe3-s4 cluster: C787 (= C136), M802 (= M151), C807 (= C156), V808 (≠ I157), G809 (= G158), T810 (≠ C159), K811 (≠ R160), Y812 (≠ F161), C813 (= C162), M853 (≠ V197)
binding heme c: N805 (≠ H154), R867 (≠ Q211), K871 (vs. gap)
binding iron/sulfur cluster: C729 (= C76), N733 (≠ H80), I754 (≠ T96), C775 (= C124), M776 (≠ N125), C778 (= C127), P782 (= P131), C783 (= C132), N800 (≠ V149), C817 (= C166), Y819 (= Y168), V821 (≠ A170), R822 (= R171), C856 (= C200), Y858 (≠ F202), C859 (= C203), T891 (vs. gap), C893 (= C215), C897 (vs. gap), T899 (≠ N220)

Sites not aligning to the query:

binding heme c: 3
binding iron/sulfur cluster: 719, 720, 721, 722, 723, 725

P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
40% identity, 45% coverage: 113:230/261 of query aligns to 56:157/205 of P18776

query
sites
P18776

Q

Q

E

N

V

V

L

F

E

A

R

Y

Y

L

L

S

L

I

L

S

C

C

N

N

H

H

C

C

E

E

N

D

P

P

P

A

C

C

V

T

R

K

V

V

C

C

P

P

T

S

K

G

A

A

T

M

F

H

K

K

R

R

A

E

D

D

G

G

I

F

V

V

M

V

D

D

Y

E

H

D

R

V

C

C

I

I

G

G

C
|
C

R

R

F

Y

C

C

M

H

A

M

G

A

C

C

P

P

Y

Y

G

G

A

A

R

P

S

Q

F

Y

N

N

F

-

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

E

T

T

R

K

G

G

V

H

V

M

E

T

K

K

C

C

T

D

F

G

C

C

S

Y

E

D

R

R

L

V

A

A

V

E

G

G

Q

K

L

K

P

P

A

I

C

C

V

V

E

E

A

S

S

C

N

P

-

L

G

R

A

A

I

L

L

D

F

F

G

G

D

P

L

I

D

D

D

E

C102 (= C159) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
36% identity, 53% coverage: 123:260/261 of query aligns to 135:258/337 of 2ivfB

query
sites
2ivfB

L
x
M

C
|
C

N
|
N

H
|
H

C
|
C

E

T

N

N

P
|
P

P

A

C
|
C

V

L

R

A

V

A

C
|
C

P

P

T
|
T

K

G

A

A

T
x
I

F

Y

K

K

R

R

A

E

D

D

-

N

G

G

I

I

V
|
V

V

L

M

V

D

D

Y

Q

H

E

R

R

C
|
C

I
x
K

G
|
G

C
x
H

R
|
R

F
x
H

C
|
C

M

V

A

E

G

A

C
|
C

P

P

Y

Y

G

K

A
|
A

R
x
I

S

Y

F

F

N

N

F

-

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

P

T

V

R

S

G

Q

V

T

V
x
S

E

E

K
|
K

C
|
C

T
x
I

F
x
L

C
|
C

S

Y

E

P

R

R

L

I

A

E

V

K

G

G

Q

I

L

A

P
x
N

A

A

C
|
C

V

N

E

R

A

Q

S
x
C

N

P

G

G

A

R

I
x
V

-
x
R

L

A

F

F

G

G

D

Y

L

L

D

D

P

T

N

T

S

S

P

H

V

V

R

H

K

K

A

L

L

V

N

K

E

K

-

W

N

K

Y

V

S

A

I

L

R

P

R

L

K

H

P

A

S

E

V

Y

G

G

T

T

Q

G

P

P

G

N

V

I

Y

Y

I

V

binding fe3-s4 cluster: C148 (= C136), T150 (= T138), I153 (≠ T141), C169 (= C156), K170 (≠ I157), G171 (= G158), H172 (≠ C159), R173 (= R160), H174 (≠ F161), C175 (= C162), S193 (≠ V197)
binding protoporphyrin ix containing fe: T150 (= T138), K170 (≠ I157), H172 (≠ C159)
binding iron/sulfur cluster: M135 (≠ L123), C136 (= C124), N137 (= N125), H138 (= H126), C139 (= C127), P142 (= P130), C144 (= C132), V162 (= V149), C179 (= C166), A183 (= A170), I184 (≠ R171), K195 (= K199), C196 (= C200), I197 (≠ T201), L198 (≠ F202), C199 (= C203), N209 (≠ P213), C211 (= C215), C215 (≠ S219), V219 (≠ I223), R220 (vs. gap)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41

5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
35% identity, 53% coverage: 123:260/261 of query aligns to 132:255/329 of 5ch7B

query
sites
5ch7B

L

M

C
|
C

N
|
N

H

H

C
|
C

E

T

N

K

P
|
P

P

A

C
|
C

V

L

R

E

V

A

C
|
C

P

P

T

N

K

E

A

A

T
x
I

F

Y

K

K

R

R

-

E

A

Q

D

D

G

G

I

I

V

V

M

I

D

H

Y

Q

H

D

R

K

C
|
C

I
x
K

G
|
G

C
x
A

R
x
Q

F
x
A

C
|
C

M

V

A

Q

G

S

C
|
C

P

P

Y

Y

G

A

A

K

R

P

S

Y

F

F

N

N

F

-

G

-

D

-

P

-

Q

-

P

P

H

L

I

T

K

N

A

K

I
x
A

N

N

P

-

K

-

F

-

P

-

A

-

R

-

T

-

R

-

G

-

V

-

E

-

K

K

C
|
C

T

I

F
x
G

C
|
C

S

F

E

P

R

R

L

I

A

E

V

Q

G

G

Q

V

L

A

P

P

A

A

C
|
C

V

V

E

A

A

Q

S
x
C

N
x
V

G
|
G

-

R

A

A

I

M

L

H

F

V

G

G

D

F

L

V

D

D

P

V

N

N

S

S

P

S

V

V

R

Y

K

K

A

L

L

I

N

K

E

Q

-

Y

N

K

Y

V

S

A

I

L

R

P

R

L

K

H

P

P

S

E

V

F

G

G

T

T

Q

E

P

P

G

N

V

V

Y

F

Y

Y

I

V

binding fe3-s4 cluster: C145 (= C136), I150 (≠ T141), C166 (= C156), K167 (≠ I157), G168 (= G158), A169 (≠ C159), Q170 (≠ R160), A171 (≠ F161), C172 (= C162), A190 (≠ I185)
binding iron/sulfur cluster: C133 (= C124), N134 (= N125), C136 (= C127), P139 (= P130), C141 (= C132), C176 (= C166), C193 (= C200), G195 (≠ F202), C196 (= C203), C208 (= C215), C212 (≠ S219), V213 (≠ N220), G214 (= G221)

Sites not aligning to the query:

binding iron/sulfur cluster: 17, 18, 19, 20, 21, 23, 27, 31, 43, 45, 130

3egwB The crystal structure of the narghi mutant narh - c16a
38% identity, 46% coverage: 123:242/261 of query aligns to 183:288/509 of 3egwB

query
sites
3egwB

L

L

C
|
C

N
x
E

H
|
H

C
|
C

E

L

N

N

P
|
P

P

A

C
|
C

V

V

R

A

V

T

C
|
C

P

P

T
x
S

K

G

A

A

T
x
I

F

Y

K

K

R

R

A

E

-

E

D

D

G

G

I

I

V

V

V

L

M

I

D

D

Y

Q

H

D

R

K

C
|
C

I
x
R

G
|
G

C
x
W

R
|
R

F

M

C
|
C

M

I

A

T

G

G

C
|
C

P

P

Y

Y

G

K

A

K

R
x
I

S

Y

F

F

N

N

F

W

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

-

T

K

R

S

G

G

V

K

V
x
S

E

E

K
|
K

C
|
C

T

I

F
|
F

C
|
C

S

Y

E

P

R

R

L

I

A

E

V

A

G

G

Q

Q

L

P

P
x
T

A

V

C
|
C

V

S

E

E

A

T

S
x
C

N

V

G
|
G

A

R

I

I

L
x
R

F

Y

G

L

D

G

-

V

-

L

L

L

D

Y

D

D

P

A

N

D

S

A

P

I

V

E

R

R

K

A

A

A

L

S

N

T

E

E

N

N

binding fe3-s4 cluster: C196 (= C136), S198 (≠ T138), I201 (≠ T141), C217 (= C156), R218 (≠ I157), G219 (= G158), W220 (≠ C159), R221 (= R160), C223 (= C162), S241 (≠ V197), C263 (≠ S219), G265 (= G221), R268 (≠ L224)
binding protoporphyrin ix containing fe: W220 (≠ C159), R221 (= R160)
binding iron/sulfur cluster: C184 (= C124), E185 (≠ N125), H186 (= H126), C187 (= C127), P190 (= P130), C192 (= C132), C227 (= C166), I232 (≠ R171), K243 (= K199), C244 (= C200), F246 (= F202), C247 (= C203), T257 (≠ P213), C259 (= C215)

Sites not aligning to the query:

binding fe3-s4 cluster: 17, 18, 19, 20, 22, 181
binding protoporphyrin ix containing fe: 88, 89
binding iron/sulfur cluster: 26, 30, 41, 42

P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 46% coverage: 123:242/261 of query aligns to 183:288/512 of P11349

query
sites
P11349

L

L

C
|
C

N

E

H

H

C
|
C

E

L

N

N

P

P

P

A

C
|
C

V

V

R

A

V

T

C
|
C

P

P

T

S

K

G

A

A

T

I

F

Y

K

K

R

R

A

E

-

E

D

D

G

G

I

I

V

V

V

L

M

I

D

D

Y

Q

H

D

R

K

C
|
C

I

R

G

G

C

W

R

R

F

M

C
|
C

M

I

A

T

G

G

C
|
C

P

P

Y

Y

G

K

A

K

R

I

S

Y

F

F

N

N

F

W

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

-

T

K

R

S

G

G

V

K

V

S

E

E

K

K

C
|
C

T

I

F

F

C
|
C

S

Y

E

P

R

R

L

I

A

E

V

A

G

G

Q

Q

L

P

P

T

A

V

C
|
C

V

S

E

E

A

T

S
x
C

N

V

G

G

A

R

I

I

L

R

F

Y

G

L

D

G

-

V

-

L

L

L

D

Y

D

D

P

A

N

D

S

A

P

I

V

E

R

R

K

A

A

A

L

S

N

T

E

E

N

N

C184 (= C124) binding [4Fe-4S] cluster
C187 (= C127) binding [4Fe-4S] cluster
C192 (= C132) binding [4Fe-4S] cluster
C196 (= C136) binding [3Fe-4S] cluster
C217 (= C156) binding [3Fe-4S] cluster
C223 (= C162) binding [3Fe-4S] cluster
C227 (= C166) binding [4Fe-4S] cluster
C244 (= C200) binding [4Fe-4S] cluster
C247 (= C203) binding [4Fe-4S] cluster
C259 (= C215) binding [4Fe-4S] cluster
C263 (≠ S219) binding [4Fe-4S] cluster

Sites not aligning to the query:

16 binding [4Fe-4S] cluster
19 binding [4Fe-4S] cluster
22 binding [4Fe-4S] cluster
26 binding [4Fe-4S] cluster

P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
40% identity, 39% coverage: 124:226/261 of query aligns to 100:187/294 of P0AAJ3

query
sites
P0AAJ3

C
|
C

N

M

H

H

C
|
C

E

E

N

D

P

P

P

G

C
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C

V

L

R

K

V

A

C
|
C

P

P

T

S

K

A

-

G

A

A

T

I

F

I

K

Q

R

Y

A

A

D

N

G

G

I

I

V

V

V

D

M

F

D

Q

Y

S

H

E

R

N

C
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C

I

I

G

G

C
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C

R

G

F

Y

C
|
C

M

I

A

A

G

G

C
|
C

P

P

Y

F

G

N

A

I

R

P

S

R

F

L

N

N

F

K

G

E

D

D

P

N

Q

R

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

-

T

-

R

-

G

-

V

-

V

V

E

Y

K

K

C
|
C

T

T

F

L

C
|
C

S

V

E

D

R

R

L

V

A

S

V

V

G

G

Q

Q

L

E

P

P

A

A

C
|
C

V

V

E

K

A

T

S
x
C

-

P

N

T

G

G

A

A

I

I

L

H

F

F

G

G

C100 (= C124) binding [4Fe-4S] cluster
C103 (= C127) binding [4Fe-4S] cluster
C108 (= C132) binding [4Fe-4S] cluster
C112 (= C136) binding [4Fe-4S] cluster
C133 (= C156) binding [4Fe-4S] cluster
C136 (= C159) binding [4Fe-4S] cluster
C139 (= C162) binding [4Fe-4S] cluster
C143 (= C166) binding [4Fe-4S] cluster
C160 (= C200) binding [4Fe-4S] cluster
C163 (= C203) binding [4Fe-4S] cluster
C175 (= C215) binding [4Fe-4S] cluster
C179 (≠ S219) binding [4Fe-4S] cluster

Sites not aligning to the query:

39 binding [4Fe-4S] cluster
42 binding [4Fe-4S] cluster
45 binding [4Fe-4S] cluster
49 binding [4Fe-4S] cluster

1kqfB Formate dehydrogenase n from e. Coli (see paper)
40% identity, 39% coverage: 124:226/261 of query aligns to 99:186/289 of 1kqfB

query
sites
1kqfB

C
|
C

N
x
M

H
|
H

C
|
C

E

E

N

D

P
|
P

P

G

C
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C

V

L

R

K

V

A

C
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C

P
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P

T

S

K

A

-

G

A

A

T
x
I

F

I

K

Q

R

Y

A

A

D

N

G

G

I

I

V
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V

V

D

M

F

D

Q

Y

S

H

E

R

N

C
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C

I
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I

G
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G

C
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C

R
x
G

F
x
Y

C
|
C

M

I

A

A

G

G

C
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C

P

P

Y

F

G

N

A
x
I

R
x
P

S

R

F

L

N

N

F

K

G

E

D

D

P

N

Q

R

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

-

T

-

R

-

G

-

V

-

V
|
V

E

Y

K
|
K

C
|
C

T

T

F
x
L

C
|
C

S

V

E

D

R

R

L

V

A

S

V

V

G

G

Q

Q

L

E

P
|
P

A

A

C
|
C

V

V

E

K

A

T

S
x
C

-
x
P

N

T

G

G

A

A

I
|
I

L

H

F

F

G

G

binding protoporphyrin ix containing fe: Y137 (≠ F161)
binding iron/sulfur cluster: C99 (= C124), M100 (≠ N125), H101 (= H126), C102 (= C127), P105 (= P130), C107 (= C132), C111 (= C136), P112 (= P137), I117 (≠ T141), V125 (= V149), C132 (= C156), I133 (= I157), G134 (= G158), C135 (= C159), G136 (≠ R160), Y137 (≠ F161), C138 (= C162), C142 (= C166), I146 (≠ A170), P147 (≠ R171), V156 (= V197), K158 (= K199), C159 (= C200), L161 (≠ F202), C162 (= C203), P172 (= P213), C174 (= C215), C178 (≠ S219), P179 (vs. gap), I183 (= I223)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 252
binding iron/sulfur cluster: 31, 38, 39, 40, 41, 42, 44, 48, 52, 77, 79

7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
36% identity, 53% coverage: 123:260/261 of query aligns to 173:305/409 of 7b04A

query
sites
7b04A

L
x
I

C
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C

N
|
N

H
|
H

C
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C

E

T

N

Y

P
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P

P

G

C
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C

V

L

R

A

V

A

C
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C

P

P

T
x
R

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K

A

A

T
x
I

F

Y

K

K

R

R

A

K

-

E

D

D

G

G

I

I

V

V

V

L

M

I

D

D

Y

Q

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R

R

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C

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x
R

G
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G

C
x
Y

R
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R

F
x
K

C
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C

M

V

A

E

G

Q

C
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C

P

P

Y

Y

G

K

A

-

R

-

S

-

F

-

N

-

F

-

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

K

P
|
P

K

M

F

Y

P

R

A

G

R

L

T

T

R

R

G

-

V

V

V
x
S

E

E

K
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K

C
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C

T

I

F
x
A

C
|
C

S

Y

E

P

R

R

-

I

-

E

-

G

-

R

-

D

-

S

L

L

A

T

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G

G

Q

R

-

P

-

M

L

E

P
x
T

A
x
R

C
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C

V

M

E

S

A

A

S
x
C

N
x
V

G

G

A

Q

I
|
I

-
x
R

L

L

F

Q

G

G

D

F

L

L

D

D

-

N

P

P

N

K

S

N

P

P

V

I

R

T

K

W

A

L

L

I

-

R

N

H

E

Q

N

K

Y

I

S

A

I

L

R

P

R

L

K

Y

P

P

S

Q

V

F

G

G

T

T

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P

P

G

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V

I

Y

Y

I

I

binding fe3-s4 cluster: C186 (= C136), I191 (≠ T141), C207 (= C156), R208 (≠ I157), G209 (= G158), Y210 (≠ C159), R211 (= R160), K212 (≠ F161), C213 (= C162), S231 (≠ V197)
binding protoporphyrin ix containing fe: R188 (≠ T138), R208 (≠ I157), Y210 (≠ C159)
binding iron/sulfur cluster: I173 (≠ L123), C174 (= C124), N175 (= N125), H176 (= H126), C177 (= C127), P180 (= P130), C182 (= C132), C217 (= C166), P222 (= P187), K233 (= K199), C234 (= C200), A236 (≠ F202), C237 (= C203), T255 (≠ P213), R256 (≠ A214), C257 (= C215), C261 (≠ S219), V262 (≠ N220), I265 (= I223), R266 (vs. gap)

Sites not aligning to the query:

binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62, 171

7qv7A Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
35% identity, 43% coverage: 124:236/261 of query aligns to 51:159/174 of 7qv7A

query
sites
7qv7A

C
|
C

N
x
R

H

H

C
|
C

E

E

N

D

P

A

P

P

C
|
C

V

A

R

E

V

V

C
|
C

P

P

T

V

K

E

A

A

T

I

F

K

K

K

R

E

A

G

D

N

G

A

I

I

V

I

M

-

D

D

Y

E

H

K

R

A

C
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C

I
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I

G
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G

C
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C

R

K

F

T

C
|
C

M

L

A

L

G

A

C
|
C

P

S

Y

F

G

G

A

A

R
x
I

S

D

F
|
F

N

S

F

V

G

Q

D

D

P

S

-

L

-

E

Q

Q

P

S

H

I

I

F

K

K

A

D

I

I

N

K

P

E

K

N

F

L

P

-

A

-

R

-

T

M

R

R

G

I

V

V

V

A

E

V

K

K

C
|
C

T

D

F
x
L

C
|
C

S

N

E

F

R

R

L

-

A

-

V

-

G

E

Q

E

L

G

P

P

A
|
A

C
|
C

V

V

E

Q

-

F

A
x
C

S

P

N

T

G

K

A

A

I

L

L

K

F

L

G

V

D

D

L

G

D

D

D

E

P

I

N

N

S

K

P

M

V

V

R

K

binding iron/sulfur cluster: C51 (= C124), R52 (≠ N125), C54 (= C127), C59 (= C132), C63 (= C136), C82 (= C156), I83 (= I157), G84 (= G158), C85 (= C159), C88 (= C162), C92 (= C166), I97 (≠ R171), F99 (= F173), C125 (= C200), L127 (≠ F202), C128 (= C203), A136 (= A214), C137 (= C215), C141 (≠ A218)

Sites not aligning to the query:

binding iron/sulfur cluster: 12, 13, 14, 15, 16, 18, 22, 26, 37

7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
37% identity, 36% coverage: 124:218/261 of query aligns to 63:153/183 of 7qv7B

query
sites
7qv7B

C
|
C

N
x
R

H
|
H

C
|
C

E

E

N

D

P
x
A

P

P

C
|
C

V

A

R

N

V

V

C
|
C

P

-

T

T

K

V

A

G

T
x
A

F
x
I

K

K

R

R

A

E

D

G

G

N

I

A

V

I

V

V

M

V

D

D

Y

E

H

K

R

L

C
|
C

I
|
I

G

G

C
|
C

R

K

F

S

C
|
C

M

L

A

L

G

A

C
|
C

P

P

Y

F

G

G

A

A

R
x
I

S

E

F

L

-

L

-

P

N

Q

F

Y

G

E

D

D

P

G

Q

R

P

-

H

E

I

V

K

F

A

Q

I

I

N

N

P

L

K

K

F

L

P

V

A

Q

R

E

T

P

R

R

G

I

V

I

V

A

E

Y

K

K

C
|
C

T

D

F
x
L

C
|
C

S

N

E

D

R

-

L

-

A

-

V

L

G

G

Q

E

L

-

P
|
P

A

A

C
|
C

V

V

E

K

A

A

binding iron/sulfur cluster: C63 (= C124), R64 (≠ N125), H65 (= H126), C66 (= C127), A69 (≠ P130), C71 (= C132), C75 (= C136), A79 (≠ T141), I80 (≠ F142), C94 (= C156), I95 (= I157), C97 (= C159), C100 (= C162), C104 (= C166), I109 (≠ R171), C139 (= C200), L141 (≠ F202), C142 (= C203), P148 (= P213), C150 (= C215)

Sites not aligning to the query:

binding iron/sulfur cluster: 14, 16, 17, 18, 19, 20, 24, 28, 48, 154, 155, 159

4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
29% identity, 52% coverage: 124:259/261 of query aligns to 68:188/274 of 4v4cB

query
sites
4v4cB

C
|
C

N
x
M

H
|
H

C
|
C

E

E

N

N

P

A

P

P

C
|
C

V

V

R

A

V

K

C

-

P

G

T

N

K

G

A

A

T

V

F

Y

K

Q

R

R

A

E

D

D

G

G

I

I

V
|
V

V

L

M

I

D

D

Y

P

H

E

R

K

C

A

I

K

G

G

C

K

R

K

F

E

C

L

M

L

A

D

G

T

C
|
C

P

P

Y

Y

G

G

A
x
V

R

M

S

Y

F

W

N

N

F

-

G

-

D

-

P

-

Q

-

P

-

H

-

I

-

K

-

A

-

I

-

N

-

P

-

K

-

F

-

P

-

A

-

R

E

T

E

R

E

G

N

V

V

V

A

E

Q

K

K

C
|
C

T

T

F
x
M

C
|
C

S

A

E

H

R

L

L

L

A

D

-

D

-

E

-

S

-

W

V

A

G

P

Q

K

L

M

P
|
P

A

R

C
|
C

V

A

E

H

A

-

S

N

N
x
C

G

G

A
x
S

I

F

L
x
V

F
x
Y

G

E

D

F

L

L

D

K

D

T

P

T

N

P

S

E

P

A

V

M

R

A

K

K

A

K

L

V

N

E

E

E

N

E

Y

G

S

L

I

E

R

V

R

I

K

K

P

P

S

E

V

L

G

G

T

T

Q

K

P

P

G

R

V

V

Y

Y

binding iron/sulfur cluster: C68 (= C124), M69 (≠ N125), H70 (= H126), C71 (= C127), C76 (= C132), V92 (= V149), C109 (= C166), V113 (≠ A170), C126 (= C200), M128 (≠ F202), C129 (= C203), P143 (= P213), C145 (= C215), C149 (≠ N220), S151 (≠ A222), V153 (≠ L224), Y154 (≠ F225)

Sites not aligning to the query:

binding calcium ion: 61, 62
binding iron/sulfur cluster: 13, 14, 15, 16, 17, 18, 19, 23

Query Sequence

>206728 MicrobesOnline__882:206728
MNNSRRRFLKIASLSVFGLGAKGVLDVAAGTTPDAAALTGKYEDGATALKAKRWAMVIDM
RKFTSPEDYSRVIEACHSIHNVPSIEGNQEIKWLWTDTYEATFPDDMNPHLSQEVLERRY
LLLCNHCENPPCVRVCPTKATFKRADGIVVMDYHRCIGCRFCMAGCPYGARSFNFGDPQP
HIKAINPKFPARTRGVVEKCTFCSERLAVGQLPACVEASNGAILFGDLDDPNSPVRKALN
ENYSIRRKPSVGTQPGVYYIV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory