SitesBLAST
Comparing 209218 MicrobesOnline__882:209218 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2q7vA Crystal structure of deinococcus radiodurans thioredoxin reductase (see paper)
45% identity, 50% coverage: 272:562/579 of query aligns to 7:304/313 of 2q7vA
- active site: P41 (≠ V306), I45 (≠ V310), E50 (≠ V315), C141 (= C400), C144 (= C403), D145 (= D404)
- binding flavin-adenine dinucleotide: G16 (= G281), P17 (= P282), A18 (= A283), E37 (≠ D302), K38 (= K303), G43 (= G308), Q44 (= Q309), I45 (≠ V310), N53 (= N318), E85 (= E346), V86 (≠ N347), T118 (= T377), G119 (= G378), C144 (= C403), G282 (= G541), D283 (= D542), Q291 (= Q549), L292 (≠ I550), S295 (≠ A553)
7aawA Thioredoxin reductase from bacillus cereus (see paper)
41% identity, 50% coverage: 274:564/579 of query aligns to 8:301/315 of 7aawA
- binding flavin-adenine dinucleotide: G13 (= G279), G15 (= G281), P16 (= P282), A17 (= A283), E36 (≠ D302), R37 (≠ K303), G42 (= G308), Q43 (= Q309), T47 (= T313), N52 (= N318), G82 (≠ E348), V84 (= V350), A111 (= A376), S112 (≠ T377), G113 (= G378), C138 (= C403), G277 (= G541), D278 (= D542), Q286 (= Q549), I287 (= I550)
- binding alpha-D-glucopyranose: R27 (= R293), D49 (≠ V315), K74 (≠ Q340), F75 (≠ Y341), P122 (= P387), G123 (= G388), E126 (≠ R391), G129 (= G394), G131 (= G396), V132 (= V397), F143 (≠ Y408), E206 (= E470), N208 (≠ M472)
7aawB Thioredoxin reductase from bacillus cereus (see paper)
41% identity, 50% coverage: 274:564/579 of query aligns to 5:298/311 of 7aawB
- binding flavin-adenine dinucleotide: G10 (= G279), G12 (= G281), P13 (= P282), A14 (= A283), E33 (≠ D302), R34 (≠ K303), G39 (= G308), Q40 (= Q309), T44 (= T313), N49 (= N318), G79 (≠ E348), D80 (≠ G349), V81 (= V350), S109 (≠ T377), G110 (= G378), Y113 (≠ W381), C135 (= C403), G274 (= G541), D275 (= D542), Q283 (= Q549), I284 (= I550)
- binding alpha-D-glucopyranose: D46 (≠ V315), E48 (= E317), G126 (= G394), G128 (= G396), D136 (= D404), A138 (≠ F406), F139 (≠ V407), F139 (≠ V407), F140 (≠ Y408)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (= G418), G151 (= G419), D152 (≠ N420), S153 (≠ T421), E156 (≠ T424), H172 (= H440), R173 (= R441), R174 (= R442), R178 (= R446), I236 (= I502)
5mh4A Crystal structure of lactococcus lactis thioredoxin reductase (fr conformation) (see paper)
40% identity, 51% coverage: 274:566/579 of query aligns to 3:298/303 of 5mh4A
- active site: V34 (≠ I305), P35 (≠ V306), M39 (≠ V310), E44 (≠ V315), C130 (= C400), C133 (= C403), D134 (= D404)
- binding flavin-adenine dinucleotide: G10 (= G281), P11 (= P282), A12 (= A283), E31 (≠ D302), R32 (≠ K303), G37 (= G308), Q38 (= Q309), T42 (= T313), N47 (= N318), G77 (≠ Q340), V79 (≠ A342), T107 (= T377), G108 (= G378), E155 (≠ D425), V239 (≠ N507), F242 (≠ Q510), G272 (= G541), D273 (= D542), Q281 (= Q549), I282 (= I550)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I146 (≠ V416), G147 (= G417), G148 (= G418), G149 (= G419), D150 (≠ N420), S151 (≠ T421), E154 (≠ T424), H170 (= H440), R171 (= R441), R172 (= R442), R176 (= R446), V234 (≠ I502), G235 (= G503), R280 (= R548), Q281 (= Q549)
5uwyA The crystal structure of thioredoxin reductase from streptococcus pyogenes mgas5005
40% identity, 51% coverage: 274:569/579 of query aligns to 6:304/306 of 5uwyA
- binding flavin-adenine dinucleotide: G13 (= G281), P14 (= P282), A15 (= A283), E34 (≠ D302), Q35 (≠ K303), G40 (= G308), Q41 (= Q309), T45 (= T313), N50 (= N318), V82 (≠ I344), T110 (= T377), G111 (= G378), Y114 (≠ W381), C136 (= C403), V242 (≠ N507), G275 (= G541), D276 (= D542), Q284 (= Q549), I285 (= I550)
3ctyB Crystal structure of t. Acidophilum thioredoxin reductase (see paper)
41% identity, 51% coverage: 274:567/579 of query aligns to 6:302/305 of 3ctyB
- active site: A38 (≠ V306), T42 (≠ V310), L47 (≠ V315), N50 (= N318), C133 (= C400), C136 (= C403), D137 (= D404)
- binding flavin-adenine dinucleotide: V10 (≠ L278), G11 (= G279), A15 (= A283), D34 (= D302), K35 (= K303), G40 (= G308), L41 (≠ Q309), T42 (≠ V310), A45 (≠ T313), P46 (= P314), N50 (= N318), V82 (= V350), T110 (= T377), G111 (= G378), S154 (≠ T421), Q241 (≠ N507), G275 (= G541), D276 (= D542), A283 (≠ R548), Q284 (= Q549), I285 (= I550)
P9WHH1 Thioredoxin reductase; TR; TRXR; EC 1.8.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
41% identity, 53% coverage: 274:579/579 of query aligns to 16:320/335 of P9WHH1
- SGPA 22:25 (≠ AGPA 280:283) binding
- Y32 (= Y290) modified: Phosphotyrosine; by PtkA; mutation to A: Significantly reduces phosphorylation.
- 44:51 (vs. 302:309, 25% identical) binding
- N60 (= N318) binding
- V93 (= V350) binding
- C145 (= C400) modified: Disulfide link with 148, Redox-active
- C148 (= C403) modified: Disulfide link with 145, Redox-active
- S166 (≠ T421) binding
- H185 (= H440) binding
- R191 (= R446) binding
- I248 (= I502) binding
- Y268 (≠ S523) binding
- D288 (= D542) binding
- R295 (= R548) binding
- RQAV 295:298 (≠ RQIV 548:551) binding
2a87A Crystal structure of m. Tuberculosis thioredoxin reductase (see paper)
41% identity, 53% coverage: 274:579/579 of query aligns to 7:311/313 of 2a87A
- active site: F39 (≠ V306), L43 (≠ V310), D48 (≠ V315), C136 (= C400), C139 (= C403), D140 (= D404)
- binding flavin-adenine dinucleotide: G12 (= G279), S13 (≠ A280), G14 (= G281), P15 (= P282), A16 (= A283), F34 (≠ L301), E35 (≠ D302), G36 (≠ K303), G40 (= G307), G41 (= G308), A42 (≠ Q309), L43 (≠ V310), T46 (= T313), V49 (= V316), N51 (= N318), D83 (≠ G349), V84 (= V350), M113 (≠ T377), C139 (= C403), G278 (= G541), D279 (= D542), R286 (= R548), Q287 (= Q549), A288 (≠ I550), V289 (= V551)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L120 (= L384), G155 (= G419), D156 (≠ N420), S157 (≠ T421), H176 (= H440), R177 (= R441), R178 (= R442), R182 (= R446), I239 (= I502), Y259 (≠ S523), R283 (vs. gap), R286 (= R548)
P0A9P4 Thioredoxin reductase; TRXR; EC 1.8.1.9 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 51% coverage: 272:564/579 of query aligns to 6:310/321 of P0A9P4
- 36:43 (vs. 302:309, 38% identical) binding
- C136 (= C400) modified: Disulfide link with 139, Redox-active
- C139 (= C403) modified: Disulfide link with 136, Redox-active
- 287:296 (vs. 542:550, 40% identical) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
4gcmB Crystal structure of a thioredoxine reductase (trxb) from staphylococcus aureus subsp. Aureus mu50 at 1.80 a resolution
39% identity, 51% coverage: 274:570/579 of query aligns to 6:305/309 of 4gcmB
- active site: C133 (= C400), C136 (= C403), D137 (= D404)
- binding flavin-adenine dinucleotide: G11 (= G279), G13 (= G281), P14 (= P282), A15 (= A283), E34 (≠ D302), R35 (≠ K303), G40 (= G308), Q41 (= Q309), T45 (= T313), N50 (= N318), D81 (≠ G349), I82 (≠ V350), T110 (= T377), G111 (= G378), C136 (= C403), G275 (= G541), D276 (= D542), Q284 (= Q549), I285 (= I550)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I117 (≠ L384), G151 (= G418), G152 (= G419), D153 (≠ N420), S154 (≠ T421), E157 (≠ T424), R174 (= R441), R175 (= R442), R179 (= R446), Q181 (≠ E448), I237 (= I502)
5u63B Crystal structure of putative thioredoxin reductase from haemophilus influenzae
41% identity, 52% coverage: 264:564/579 of query aligns to 1:312/319 of 5u63B
- active site: C139 (= C400), C142 (= C403), D143 (= D404)
- binding flavin-adenine dinucleotide: G16 (= G279), S17 (≠ A280), G18 (= G281), P19 (= P282), A20 (= A283), T39 (≠ D302), G40 (≠ K303), Q42 (≠ I305), G45 (= G308), Q46 (= Q309), L47 (≠ V310), T50 (= T313), N55 (= N318), H87 (≠ G349), I88 (≠ V350), A115 (= A376), T116 (= T377), G117 (= G378), H248 (= H504), G288 (= G541), D289 (= D542), R296 (= R548), Q297 (= Q549), A298 (≠ I550), S301 (≠ A553)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R121 (= R382), G157 (= G418), H179 (= H440), R180 (= R441), R181 (= R442), I246 (= I502), G247 (= G503), H248 (= H504), R296 (= R548)
1f6mA Crystal structure of a complex between thioredoxin reductase, thioredoxin, and the NADP+ analog, aadp+ (see paper)
43% identity, 51% coverage: 272:564/579 of query aligns to 5:309/320 of 1f6mA
- active site: S135 (≠ C400), C138 (= C403), D139 (= D404)
- binding 3-aminopyridine-adenine dinucleotide phosphate: L119 (= L384), G153 (= G418), G154 (= G419), N155 (= N420), T156 (= T421), E159 (≠ T424), H175 (= H440), R176 (= R441), R177 (= R442), R181 (= R446), I243 (= I502), G244 (= G503), H245 (= H504), R293 (= R548), Q294 (= Q549)
- binding flavin-adenine dinucleotide: G12 (= G279), S13 (≠ A280), G14 (= G281), P15 (= P282), A16 (= A283), T35 (≠ D302), G36 (≠ K303), E38 (≠ I305), G41 (= G308), Q42 (= Q309), L43 (≠ V310), T46 (= T313), V49 (= V316), N51 (= N318), H83 (≠ G349), I84 (≠ V350), A111 (= A376), T112 (= T377), G113 (= G378), H245 (= H504), G285 (= G541), D286 (= D542), R293 (= R548), Q294 (= Q549), A295 (≠ I550), S298 (≠ A553)
1tdfA Crystal structure of escherichia coli thioredoxin reductase refined at 2 angstrom resolution: implications for a large conformational change during catalysis (see paper)
43% identity, 51% coverage: 272:564/579 of query aligns to 5:309/316 of 1tdfA
- active site: C135 (= C400), S138 (≠ C403), D139 (= D404)
- binding flavin-adenine dinucleotide: G12 (= G279), S13 (≠ A280), G14 (= G281), P15 (= P282), A16 (= A283), T35 (≠ D302), G36 (≠ K303), E38 (≠ I305), G41 (= G308), Q42 (= Q309), L43 (≠ V310), T46 (= T313), V49 (= V316), N51 (= N318), H83 (≠ G349), I84 (≠ V350), A111 (= A376), T112 (= T377), S138 (≠ C403), G285 (= G541), D286 (= D542), R293 (= R548), Q294 (= Q549), A295 (≠ I550), S298 (≠ A553)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L119 (= L384), I151 (≠ V416), T156 (= T421), E159 (≠ T424), H175 (= H440), R176 (= R441), R181 (= R446), E183 (= E448), I243 (= I502), G244 (= G503), H290 (≠ G546), R293 (= R548)
8ccmA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with compound 2-06
41% identity, 53% coverage: 274:577/579 of query aligns to 4:305/305 of 8ccmA
- binding flavin-adenine dinucleotide: I8 (≠ L278), G9 (= G279), S10 (≠ A280), G11 (= G281), P12 (= P282), A13 (= A283), E32 (≠ D302), G33 (≠ K303), Q35 (≠ I305), G38 (= G308), A39 (≠ Q309), L40 (≠ V310), T43 (= T313), N48 (= N318), D80 (≠ G349), V81 (= V350), M109 (≠ T377), G110 (= G378), T131 (≠ Y399), C135 (= C403), G274 (= G541), D275 (= D542), R282 (= R548), Q283 (= Q549), A284 (≠ I550), A287 (= A553)
- binding ~{N}6-(4-aminophenyl)-1,2-benzothiazole-3,6-diamine: R114 (= R382), H115 (≠ K383), L116 (= L384), R173 (= R441), E200 (≠ V467), I201 (≠ V468), I235 (= I502)
8cclA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry a09
41% identity, 53% coverage: 274:577/579 of query aligns to 4:305/305 of 8cclA
- binding flavin-adenine dinucleotide: I8 (≠ L278), G9 (= G279), S10 (≠ A280), G11 (= G281), P12 (= P282), A13 (= A283), E32 (≠ D302), G33 (≠ K303), Q35 (≠ I305), G38 (= G308), A39 (≠ Q309), L40 (≠ V310), T43 (= T313), N48 (= N318), D80 (≠ G349), V81 (= V350), M109 (≠ T377), G110 (= G378), T131 (≠ Y399), C135 (= C403), G274 (= G541), D275 (= D542), R282 (= R548), Q283 (= Q549), A284 (≠ I550), A287 (= A553)
- binding [1,2]thiazolo[5,4-b]pyridin-3-amine: L116 (= L384), R173 (= R441), E200 (≠ V467), I201 (≠ V468)
8cckA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry h07
41% identity, 53% coverage: 274:577/579 of query aligns to 4:305/305 of 8cckA
- binding flavin-adenine dinucleotide: G9 (= G279), S10 (≠ A280), G11 (= G281), P12 (= P282), A13 (= A283), E32 (≠ D302), G33 (≠ K303), Q35 (≠ I305), G38 (= G308), A39 (≠ Q309), L40 (≠ V310), T43 (= T313), N48 (= N318), D80 (≠ G349), V81 (= V350), M109 (≠ T377), G110 (= G378), T131 (≠ Y399), C135 (= C403), G274 (= G541), D275 (= D542), R282 (= R548), Q283 (= Q549), A284 (≠ I550), A287 (= A553)
- binding ~{N}-(4-hydroxyphenyl)-2-pyrazol-1-yl-ethanamide: R114 (= R382), H115 (≠ K383), L116 (= L384), V148 (= V416), R173 (= R441), E200 (≠ V467), I201 (≠ V468)
8ccjA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with NADPH
41% identity, 53% coverage: 274:577/579 of query aligns to 4:305/305 of 8ccjA
- binding flavin-adenine dinucleotide: I8 (≠ L278), G9 (= G279), S10 (≠ A280), G11 (= G281), P12 (= P282), A13 (= A283), E32 (≠ D302), G33 (≠ K303), Q35 (≠ I305), G38 (= G308), A39 (≠ Q309), L40 (≠ V310), T43 (= T313), N48 (= N318), D80 (≠ G349), V81 (= V350), M109 (≠ T377), G110 (= G378), T131 (≠ Y399), C135 (= C403), G274 (= G541), D275 (= D542), R282 (= R548), Q283 (= Q549), A284 (≠ I550), A287 (= A553)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (= G418), G151 (= G419), D152 (≠ N420), S153 (≠ T421), E156 (≠ T424), H172 (= H440), R173 (= R441), R174 (= R442), R178 (= R446), I235 (= I502)
5uthA Crystal structure of thioredoxin reductase from mycobacterium smegmatis in complex with fad
41% identity, 53% coverage: 274:577/579 of query aligns to 5:306/306 of 5uthA
- active site: C133 (= C400), C136 (= C403), D137 (= D404)
- binding flavin-adenine dinucleotide: I9 (≠ L278), G10 (= G279), S11 (≠ A280), G12 (= G281), P13 (= P282), A14 (= A283), F32 (≠ L301), E33 (≠ D302), G34 (≠ K303), Q36 (≠ I305), G39 (= G308), A40 (≠ Q309), L41 (≠ V310), N49 (= N318), D81 (≠ G349), V82 (= V350), M110 (≠ T377), G111 (= G378), C136 (= C403), G275 (= G541), D276 (= D542), R283 (= R548), Q284 (= Q549), A285 (≠ I550), A288 (= A553)
3f8rA Crystal structure of sulfolobus solfataricus thioredoxin reductase b3 in complex with two NADP molecules (see paper)
38% identity, 50% coverage: 274:565/579 of query aligns to 8:302/308 of 3f8rA
- active site: C133 (= C400), C136 (= C403), D137 (= D404)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V12 (≠ L278), G13 (= G279), L14 (≠ A280), G15 (= G281), P16 (= P282), A17 (= A283), E37 (≠ K303), T38 (≠ I305), G41 (= G308), Q42 (= Q309), I82 (≠ G349), V83 (= V350), G109 (≠ A376), I110 (≠ T377), G111 (= G378), R115 (= R382), L117 (= L384), D153 (≠ N420), S154 (≠ T421), E157 (≠ T424), R174 (= R441), R175 (= R442), Y184 (≠ L451), I236 (= I502), G275 (= G541), D276 (= D542), L282 (vs. gap), G283 (= G546), R285 (= R548)
5vt3B High resolution structure of thioredoxin-disulfide reductase from vibrio vulnificus cmcp6 in complex with NADP and fad
40% identity, 52% coverage: 265:564/579 of query aligns to 1:311/319 of 5vt3B
- active site: C138 (= C400), C141 (= C403), D142 (= D404)
- binding flavin-adenine dinucleotide: G15 (= G279), S16 (≠ A280), G17 (= G281), P18 (= P282), A19 (= A283), T38 (≠ D302), G39 (≠ K303), Q41 (≠ I305), G44 (= G308), Q45 (= Q309), L46 (≠ V310), T49 (= T313), N54 (= N318), H86 (≠ G349), I87 (≠ V350), S114 (≠ A376), T115 (= T377), G116 (= G378), E162 (≠ T424), H247 (= H504), G287 (= G541), D288 (= D542), R295 (= R548), Q296 (= Q549), A297 (≠ I550), S300 (≠ A553)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L122 (= L384), G156 (= G418), G157 (= G419), N158 (= N420), T159 (= T421), H178 (= H440), R179 (= R441), R180 (= R442), R184 (= R446), I245 (= I502), G246 (= G503), R295 (= R548), Q296 (= Q549)
Query Sequence
>209218 MicrobesOnline__882:209218
MSGLFKKKETAAQAADTQTQTPTPEAHEWLIPEEGRDQLQKVFAGLPGDVTIEVYTGGDP
KNLFDDYARRFVADLALLAPRIKPAFHGLDSDMARKRGVTLSPTLLFNPEEYRIRFTGAP
LGEEARAFIEAIFLVSARTSGLSQAAKTILAQLDGARSPRVFSSPGCPYCPAQCANAIKC
AIERPDLVSAECVNADEFPDLSARYGVGSVPHTQFDESYKGIGLMPEERFVVELVALKDA
ERWLNEHAAKGGAHGAGTGQGAGSDAGVEVTETDLLVLGAGPAGLSAAIYAERSGLATVV
LDKGIVGGQVTVTPVVENYPGFADIAGIKLVEVLSSHARQYATIRENEGVDDIKLGRRIE
VHTPRNVFLARAVLFATGAQWRKLDVPGEDRFYGKGVSYCASCDGFVYRGRKVAVVGGGN
TALTDALHLRNLGVEVTVIHRRDTFRAEKALQDSLTREGIPVIWNAVVEEVMGDTEVRGV
RLRDTKTGETRDVPFDGVFVAIGHVPNSEQAADLGVDLEPDGSIKVDRHMRTNIPRVYAA
GDVIGGVRQIVTAVGSGATAALSAFEDLQQPRWKKDKSA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory