SitesBLAST
Comparing 3609639 Dshi_3022 PfkB domain protein (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3uboA The crystal structure of adenosine kinase from sinorhizobium meliloti
47% identity, 97% coverage: 9:332/333 of query aligns to 3:333/338 of 3uboA
- active site: R124 (= R130), G274 (= G278), A275 (= A279), G276 (= G280), D277 (= D281)
- binding adenosine: N10 (= N16), I12 (= I18), D14 (= D20), M34 (= M40), G57 (= G63), G58 (= G64), S59 (= S65), N62 (= N68), M126 (= M132), T128 (= T134), E155 (= E161), Y157 (= Y163), G274 (= G278), D277 (= D281), P313 (≠ A317)
- binding adenosine-5'-diphosphate: N214 (= N219), T244 (= T249), S246 (= S251), E247 (≠ G252), G249 (≠ P254), L266 (≠ V271), A275 (= A279), G276 (= G280), N301 (≠ V305), A304 (≠ G308), I308 (= I312)
4lc4A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with guanosine
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4lc4A
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
- binding guanosine: T245 (= T249), S247 (= S251), E248 (≠ G252), A305 (≠ G308), I309 (= I312)
4kbeA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with benzoguanamine
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4kbeA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), D278 (= D281), P314 (≠ A317)
- binding 6-phenyl-1,3,5-triazine-2,4-diamine: I17 (≠ L22), S18 (≠ A23), R19 (≠ Q24), P52 (≠ D57), A53 (≠ R58), L54 (≠ V59)
4kanA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 2-(2,5-dimethyl-1,3-thiazol-4-yl)acetic acid
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4kanA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
- binding (2,5-dimethyl-1,3-thiazol-4-yl)acetic acid: I16 (≠ V21), A43 (= A48), E44 (= E49), Y47 (= Y52), R114 (= R119), M116 (= M121), N215 (= N219), T245 (= T249), S247 (= S251), A276 (= A279)
4kalA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with quinoline-3-carboxylic acid
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4kalA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281)
- binding quinoline-3-carboxylic acid: S190 (≠ P195), F191 (= F196), D194 (= D199), S221 (≠ A225), Q224 (≠ E228)
4kahA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 4-bromo-1h-pyrazole
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4kahA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
- binding 4-bromo-1H-pyrazole: I16 (≠ V21), Y47 (= Y52), L86 (= L91)
4kadA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n1-(2.3-dihydro-1h-inden-5-yl)acetam
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4kadA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding N-(4,7-dihydro-1H-inden-6-yl)acetamide: S247 (= S251), E248 (≠ G252), A305 (≠ G308), I309 (= I312)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281)
4k9iA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with norharmane
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4k9iA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281)
- binding Norharmane: S190 (≠ P195), F191 (= F196), D194 (= D199), Q224 (≠ E228)
4k9cA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n-(hydroxymethyl)benzamide and 4-methyl-3,4- dihydro-2h-1,4-benzoxazine-7-carboxylic acid
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4k9cA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding N-(hydroxymethyl)benzamide: Y47 (= Y52), Q85 (≠ T90), L86 (= L91), D88 (≠ R93), P111 (= P116), R114 (= R119)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
- binding 4-methyl-3,4-dihydro-2H-1,4-benzoxazine-7-carboxylic acid: S190 (≠ P195), F191 (= F196), D194 (= D199), Q224 (≠ E228)
4k93A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n-(hydroxymethyl)benzamide
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4k93A
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding N-(hydroxymethyl)benzamide: L37 (= L42), I38 (≠ V43), Y47 (= Y52), Q85 (≠ T90), D88 (≠ R93), R114 (= R119), L131 (= L136), D161 (= D166), S190 (≠ P195), F191 (= F196), D194 (= D199), R197 (= R202), L220 (≠ M224), S221 (≠ A225), Q224 (≠ E228), D226 (≠ E230), R234 (= R238)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
4k8tA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with ethyl 3,4-diaminobenzoate
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4k8tA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281), P314 (≠ A317)
- binding ethyl 3,4-diaminobenzoate: F109 (≠ E114), P111 (= P116), R114 (= R119), Y130 (= Y135)
4k8cA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with adp
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4k8cA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281)
- binding adenosine-5'-diphosphate: N215 (= N219), T245 (= T249), S247 (= S251), E248 (≠ G252), G250 (≠ P254), A264 (≠ V268), A276 (= A279), G277 (= G280), C302 (≠ V305), A305 (≠ G308), I309 (= I312)
4jkuA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with quinaldic acid, nysgrc target 14306
43% identity, 98% coverage: 9:333/333 of query aligns to 4:330/330 of 4jkuA
- active site: R125 (= R130), G275 (= G278), A276 (= A279), G277 (= G280), D278 (= D281)
- binding adenosine: N11 (= N16), I13 (= I18), D15 (= D20), M35 (= M40), G58 (= G63), G59 (= G64), S60 (= S65), N63 (= N68), M127 (= M132), T129 (= T134), E156 (= E161), Y158 (= Y163), T274 (= T277), D278 (= D281)
- binding quinoline-2-carboxylic acid: S190 (≠ P195), F191 (= F196), D194 (= D199), S221 (≠ A225), Q224 (≠ E228)
4lbxA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with cytidine
43% identity, 98% coverage: 9:333/333 of query aligns to 5:331/331 of 4lbxA
- active site: R126 (= R130), G276 (= G278), A277 (= A279), G278 (= G280), D279 (= D281)
- binding adenosine: N12 (= N16), I14 (= I18), D16 (= D20), M36 (= M40), G59 (= G63), G60 (= G64), S61 (= S65), N64 (= N68), M128 (= M132), T130 (= T134), E157 (= E161), Y159 (= Y163), T275 (= T277), D279 (= D281), P315 (≠ A317)
- binding 4-amino-1-beta-d-ribofuranosyl-2(1h)-pyrimidinone: T246 (= T249), S248 (= S251), G251 (≠ P254), C303 (≠ V305), A306 (≠ G308), I310 (= I312)
4k8kA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 1-(4-methoxyphenyl)-1-cyclopropane and 2- aminoperimidine
43% identity, 98% coverage: 9:333/333 of query aligns to 5:331/331 of 4k8kA
- active site: R126 (= R130), G276 (= G278), A277 (= A279), G278 (= G280), D279 (= D281)
- binding 1-(4-methoxyphenyl)cyclopropanecarboxylic acid: A41 (≠ R45), A44 (= A48), E45 (= E49), Y48 (= Y52), P112 (= P116), R115 (= R119), M117 (= M121), Y131 (= Y135)
- binding 1H-perimidin-2-amine: A84 (≠ D88), D85 (= D89), D89 (≠ R93)
- binding adenosine: N12 (= N16), I14 (= I18), D16 (= D20), M36 (= M40), G59 (= G63), G60 (= G64), S61 (= S65), N64 (= N68), M128 (= M132), T130 (= T134), E157 (= E161), Y159 (= Y163), T275 (= T277), D279 (= D281), P315 (≠ A317)
4k8pA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 2-ethylbenzyl alcohol
43% identity, 98% coverage: 9:333/333 of query aligns to 6:332/332 of 4k8pA
- active site: R127 (= R130), G277 (= G278), A278 (= A279), G279 (= G280), D280 (= D281)
- binding (2-ethylphenyl)methanol: A45 (= A48), Y49 (= Y52), R116 (= R119)
- binding adenosine: N13 (= N16), I15 (= I18), D17 (= D20), M37 (= M40), G60 (= G63), G61 (= G64), S62 (= S65), N65 (= N68), M129 (= M132), T131 (= T134), E158 (= E161), Y160 (= Y163), T276 (= T277), D280 (= D281), P316 (≠ A317)
1liiA Structure of t. Gondii adenosine kinase bound to adenosine 2 and amp- pcp (see paper)
31% identity, 93% coverage: 8:316/333 of query aligns to 2:326/331 of 1liiA
- active site: R126 (= R130), D291 (= D281)
- binding phosphomethylphosphonic acid adenylate ester: R126 (= R130), T251 (= T249), G253 (≠ S251), H254 (≠ G252), V275 (≠ P273), T286 (≠ A276), N287 (≠ T277), G288 (= G278), A289 (= A279), G290 (= G280), D291 (= D281), N315 (≠ V305), A318 (≠ G308), Q319 (≠ S309)
- binding adenosine: N10 (= N16), I12 (= I18), D14 (= D20), G58 (= G63), G59 (= G64), S60 (= S65), C117 (≠ M121), T130 (= T134), Y159 (= Y163), N287 (≠ T277), D291 (= D281)
1lijA Structure of t. Gondii adenosine kinase bound to prodrug 2 7- iodotubercidin and amp-pcp (see paper)
32% identity, 93% coverage: 8:316/333 of query aligns to 2:325/330 of 1lijA
- active site: R126 (= R130), D290 (= D281)
- binding phosphomethylphosphonic acid adenylate ester: R126 (= R130), T250 (= T249), G252 (≠ S251), H253 (≠ G252), V274 (≠ P273), G287 (= G278), A288 (= A279), G289 (= G280), N314 (≠ V305), A317 (≠ G308), Q318 (≠ S309)
- binding 2-ribofuranosyl-3-iodo-2,3-dihydro-1h-pyrazolo[3,4-d]pyrimidin-4-ylamine: N10 (= N16), D14 (= D20), T35 (≠ Q41), G59 (= G64), S60 (= S65), N63 (= N68), T130 (= T134), Y159 (= Y163), D290 (= D281)
1dgmA Crystal structure of adenosine kinase from toxoplasma gondii (see paper)
32% identity, 93% coverage: 8:316/333 of query aligns to 3:336/346 of 1dgmA
- active site: R127 (= R130), G298 (= G278), A299 (= A279), G300 (= G280), D301 (= D281)
- binding adenosine: N11 (= N16), D15 (= D20), G60 (= G64), S61 (= S65), Y160 (= Y163), D301 (= D281)
- binding magnesium ion: A176 (= A181), I179 (≠ A184), A182 (≠ G186)
2aa0A Crystal structure of t. Gondii adenosine kinase complexed with 6- methylmercaptopurine riboside (see paper)
31% identity, 93% coverage: 8:316/333 of query aligns to 3:344/351 of 2aa0A
- active site: R127 (= R130), G306 (= G278), A307 (= A279), G308 (= G280), D309 (= D281)
- binding 2-hydroxymethyl-5-(6-methylsulfanyl-purin-9-yl)-tetrahydro-furan-3,4-diol: N11 (= N16), I13 (= I18), D15 (= D20), G60 (= G64), N64 (= N68), T131 (= T134), L133 (= L136), Y160 (= Y163), G271 (≠ S251), H272 (≠ G252), V293 (≠ P273), V296 (vs. gap), A307 (= A279), G308 (= G280), D309 (= D281), N333 (≠ V305), A336 (≠ G308), Q337 (≠ S309)
Sites not aligning to the query:
Query Sequence
>3609639 Dshi_3022 PfkB domain protein (RefSeq)
MSDTSKDPYQVVGIGNAIVDVLAQTDDSFLDHMGIEKGIMQLVERPRAEMLYAAMSDRVQ
APGGSVANTLAGLGELGLRCAFIGRVKDDTLGRFYAQGMEAEGTAFPNPPQQVEAPTSRS
MIFVTPDGERSMNTYLGAGADLGPEDVPEAVFAQVGLLFLEGYLFDKVPGKAAFEAAARY
ARGAGGHAGITLSDPFCVDRHRADFQRLVADELNFVIGNEHEYMALYETEDLGAALARAA
SVCELVVCTRSGDPVVMIRGDARVEVPVTRVVPVDATGAGDQFAAGFLYGLATGKSLEVC
GRMGVIAGSEVISHFGARPKTDVRARFVAEGLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory