SitesBLAST

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
40% identity, 100% coverage: 1:380/380 of query aligns to 2:380/385 of 5hhyA

query
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binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ P73), G77 (= G74), H78 (≠ S75), W103 (≠ F100), S153 (≠ T151), D178 (= D176), V180 (= V178), Q203 (= Q201), K204 (= K202), Y255 (= Y256), T258 (= T259)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
40% identity, 100% coverage: 1:380/380 of query aligns to 7:385/392 of P21549

query
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P21549

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T9 (≠ A3) to N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
P11 (= P5) to L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584
R36 (≠ E28) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ Q33) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G39) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G74) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F100) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ M104) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ F143) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ A145) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ A149) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T151) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G154) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ A159) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ C163) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ A166) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D176) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S180) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ A195) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S198) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K202) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (= S211) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (= R226) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ Q237) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ G246) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ L275) to T: in dbSNP:rs140992177
A280 (= A276) to V: in dbSNP:rs73106685
V326 (≠ I321) to I: in dbSNP:rs115057148
I340 (≠ L335) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
40% identity, 100% coverage: 1:380/380 of query aligns to 4:382/387 of 1j04A

query
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D

A

I

I

L

Y

Y

S

S

G

G

S

S

Q

Q

K

K

C

A

L

L

S

N

C

A

V

P

P

P

G

G

L

T

S

S

P

L

V

I

S

S

F

F

S

S

P

D

A

K

A

A

V

K

E

K

K

K

L

M

K

Y

A

S

R

R

K

K

T

T

P

K

V

P

Q

F

S

S

W

F

F

Y

L

L

D

D

Q

I

S

K

L

W

V

L

M

A

G

N

Y

F

W

W

S

G

G

C

D

D

Q

D

G

Q

A

P

G

-

K

-

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

A

S

L

L

H

R

E

E

S

S

L

L

R

A

L

L

L

I

A

A

E

E

E

Q

G

G

L

L

E

E

N

N

A

S

W

W

A

R

R

Q

H

H

H

R

S

E

M

A

H

A

A

A

L

Y

L

L

R

H

D

G

G

R

L

L

E

Q

N

A

L

L

G

G

L

L

K

Q

-

L

F

F

V

V

V

K

E

D

E

P

G

A

S

L

R

R

L

L

P

P

Q

T

L

V

N

T

A

T

V

V

Y

A

I

V

P

P

E

A

G

G

V

Y

D

D

D

W

G

R

A

D

V

I

R

V

K

S

Q

Y

L

V

L

I

E

D

N

H

Y

F

N

D

L

I

E

E

I

I

G

M

A

G

G

G

L

L

G

G

A

P

L

S

A

T

G

G

K

K

A

V

W

L

R
|
R

I

I

G

G

L

L

M

L

G

G

F

C

G

N

A

A

R

T

R

R

E

E

N

N

V

V

A

D

L

R

C

V

L

T

R

E

A

A

L

L

E

R

E

A

V

A

L

L

R

Q

binding (aminooxy)acetic acid: P25 (= P20), R357 (= R355)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
38% identity, 97% coverage: 13:379/380 of query aligns to 6:365/387 of 3islA

query
sites
3islA

P

P

R

L

R

R

V

T

L

I

M

M

G

T

P

P

G

G

P

P

S

V

D

E

V

V

Y

D

P

P

E

R

V

V

L

L

A

R

A

V

Q

M

A

S

R

T

P

P

T

V

I

V

G

G

H

Q

L

F

D

D

P

P

L

A

F

F

V

T

G

G

M

I

M

M

D

N

E

E

L

T

K

M

S

E

L

M

I

L

Q

R

Y

E

A

L

F

F

Q

Q

T

T

E

K

N

N

A

R

M

W

T

A

L

Y

A

P

V

I

S

D

A

G

P
x
T

G
x
S

S
x
R

A

A

G

G

M

I

E

E

T

A

C

V

F

L

V

A

N

S

L

V

V

I

E

E

P

P

G

E

E

D

K

D

V

V

I

L

V

I

C

P

R

I

N

Y

G

G

V

R

F
|
F

G

G

E

Y

R

L

M

L

R

T

Q

E

N

I

V

A

E

E

R

R

V

Y

G

G

A

A

T

N

A

V

V

H

V

M

V

L

D

E

N

C

E

E

W

W

G

G

T

T

P

V

V

F

S

D

L

P

S

E

D

D

V

I

E

I

A

R

A

E

L

I

K

K

A

-

H

K

P

V

D

K

A

P

K

K

F

I

L

V

A

A

F

M

V

V

H

H

A

G

E

E

T
|
T

S

S

T

T

G

G

A

R

L

I

S

H

D

P

A

L

K

K

G

A

L

I

C

G

Q

E

L

A

A

C

K

R

A

T

H

E

D

D

C

A

L

L

S

F

I

I

V

V

D
|
D

A

A

V
|
V

T

A

S

T

L

I

G

G

V

C

E

E

L

V

R

K

V

V

D

D

E

E

W

W

G

K

I

I

D

D

A

A

I

A

Y

I

S

G

G

G

S

T

Q
|
Q

K
|
K

C

C

L

L

S

S

C

V

V

P

P

S

G

G

L

M

S

A

P

P

V

I

S

T

F

Y

S

N

P

E

A

R

A

V

V

A

E

D

K

V

L

I

K

A

A

A

R

R

K

N

T

R

P

P

V

I

Q

T

S

S

W

N

F

Y

L

F

D

D

Q

L

S

S

L

Q

V

L

M

E

G

D

Y

Y

W

W

S

S

G

E

D

-

Q

-

G

-

A

-

G

-

K

R

R

R

S

L

Y

N

H

H

T

T

A

E

P

A

V

T

N

T

A

M

L

L

Y

Y

A

A

L

L

H

R

E

E

S

G

L

V

R

R

L

L

L

V

A

L

E

E

G

G

L

L

E

E

N

T

A

R

W

F

A

E

R

R

H

H

H

R

S

H

M

H

H

E

A

A

L

A

L

L

R

A

D

A

G

G

L

I

E

K

N

A

L

M

G

G

L

L

K

R

F

L

V

F

V

G

E

D

G

S

S

C

R

K

L

M

P

P

Q

V

L

V

N

T

A

C

V

V

Y

E

I

I

P

P

E

G

G

G

V

I

D

D

D

G

G

E

A

S

V

V

R

R

K

D

Q

M

L

L

E

A

N

Q

Y

F

N

G

L

I

E

E

I

I

G

A

A

S

G

S

L

F

G

G

A

P

L

L

A

A

G

G

K

K

A

I

W

W

R

R

I

I

G

G

L

T

M

M

G

G

F

Y

G

S

A

C

R

R

R

K

E

E

N

N

V

V

A

L

L

F

C

V

L

L

R

A

A

G

L

L

E

E

E

A

V

V

L

L

binding pyridoxal-5'-phosphate: T66 (≠ P73), S67 (≠ G74), R68 (≠ S75), F93 (= F100), T143 (= T151), D168 (= D176), V170 (= V178), Q193 (= Q201), K194 (= K202)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
40% identity, 98% coverage: 9:380/380 of query aligns to 13:378/383 of 3kgxA

query
sites
3kgxA

P

P

F

L

N

S

P

V

P

P

R

T

R

R

V

L

L

L

M

L

G

G

P

P

G

G

P

P

S

S

D

N

V

L

Y

A

P

P

E

R

V

V

L

L

A

A

Q

G

A

S

R

L

P

R

T

M

I

I

G

G

H

H

L

M

D

Q

P

K

L

E

F

M

V

L

G

Q

M

I

M

M

D

E

E

E

L

I

K

K

S

Q

L

G

I

I

Q

Q

Y

Y

A

V

F

F

Q

Q

T

T

E

R

N

N

A

P

M

L

T

T

L

L

A

V

V

V

S

S

A

G

P

S

G

G

S

H

A

C

G

A

M

M

E

E

T

T

C

A

F

L

V

F

N

N

L

L

V

L

E

E

P

P

G

G

E

D

K

S

V

F

I

L

V

T

C

G

R

T

N

N

G

G

V

I

F

W

G

G

E

M

R

R

M

A

R

A

Q

E

N

I

V

A

E

D

R

R

V

I

G

G

A

A

T

R

A

V

V

H

V

Q

V

M

D

I

N

K

E

K

W

P

G

G

T

E

P

H

V

Y

S

T

L

L

S

Q

D

E

V

V

E

E

A

E

A

G

L

L

K

A

A

Q

H

H

P

K

D

P

A

V

K

-

F

L

L

L

A

F

F

L

V

V

H

H

A

G

E

E

T

S

S

S

T

T

G

G

A

V

L

V

S

Q

D

P

A

L

K

D

G

G

L

F

C

G

Q

E

L

L

A

C

K

H

A

R

H

Y

D

Q

C

C

L

L

S

L

I

L

V

V

D

D

A

S

V

V

T

A

S

S

L

L

G

G

V

V

E

P

L

I

R

Y

V

M

D

D

E

Q

W

Q

G

G

I

I

D

D

A

I

I

M

Y

Y

S

S

G

S

S

S

Q

Q

K

K

C

V

L

L

S

N

C

A

V

P

P

P

G

G

L

I

S

S

P

L

V

I

S

S

F

F

S

N

P

D

A

K

A

A

V

K

E

Y

K

K

L

V

K

Y

A

S

R

R

K

K

T

T

P

K

V

P

Q

V

S

S

W

F

F

Y

L

T

D

D

Q

I

S

T

L

Y

V

L

M

A

G

K

Y

L

W

W

S

-

G

-

D

-

Q

-

G

-

A

-

G

G

G

C

K

E

R

G

S

V

Y

I

H

H

T

T

A

T

P

P

V

V

N

T

A

S

L

L

Y

Y

A

C

L

L

H

R

E

E

S

S

L

L

R

A

L

L

L

I

A

A

E

E

E

Q

G

G

L

L

E

E

N

N

A

C

W

W

A

R

R

R

H

H

H

R

S

E

M

A

H

T

A

A

L
x
H

L

L

R

H

D

K

G
x
H

L

L

E

Q

N

E

L

M

G

G

L

L

K

K

F

F

V

F

V

V

E

K

E

D

G

P

S

E

-

I

R

R

L

L

P

P

Q

T

L

I

N

T

A

T

V

V

Y

T

I

V

P

P

E

A

G

G

V

Y

D

N

D

W

G

R

A

D

V

I

R

V

K

S

Q

Y

L

V

L

L

E

D

N

H

Y

F

N

S

L

I

E

E

I

I

G

S

A

G

G

G

L

L

G

G

A

P

L

T

A

E

G

E

K

R

A

V

W

L

R

R

I

I

G

G

L

L

M

L

G

G

F

Y

G

N

A

A

R

T

E

E

N

N

V

V

A
x
D

L

R

C

V

L

A

R

E

A

A

L

L

E

R

E

E

V

A

L

L

R

Q

binding magnesium ion: H290 (≠ L293), H294 (≠ G297), D367 (≠ A369)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
40% identity, 100% coverage: 1:380/380 of query aligns to 4:380/385 of 1h0cA

query
sites
1h0cA

M

L

L

V

A

T

A

P

P

P

K

K

-

A

-

L

V

L

T

K

P

P

F

L

N

S

P

I

P

P

R

N

R

Q

V

L

L

L

M

L

G

G

P
|
P

G
|
G

P

P

S

S

D

N

V

L

Y

P

P

P

E

R

V

I

L

M

A

A

Q

G

A

G

R

L

P

Q

T

M

I

I

G

G

H

S

L

M

D

S

P

K

L

D

F

M

V

Y

G

Q

M

I

M

M

D

D

E

E

L

I

K

K

S

E

L

G

I

I

Q

Q

Y

Y

A

V

F

F

Q

Q

T

T

E

R

N

N

A

P

M

L

T

T

L

L

A

V

V

I

S

S

A

G

P
x
S

G
|
G

S
x
H

A

C

G

A

M

L

E

E

T

A

C

A

F

L

V

V

N

N

L

V

V

L

E

E

P

P

G

G

E

D

K

S

V

F

I

L

V

V

C

G

R

A

N

N

G

G

V

I

F
x
W

G

G

E

Q

R

R

M

A

R

V

Q

D

N

I

V

G

E

E

R

R

V

I

G

G

A

V

T

H

A

P

V

M

V

T

V

K

D

D

N

P

E

-

W

-

G

G

T

G

P

H

V

Y

S

T

L

L

S

Q

D

E

V

V

E

E

A

E

A

G

L

L

K

A

A

Q

H

H

P

K

D

P

A

V

K

-

F

L

L

L

A

F

F

L

V

T

H

H

A

G

E

E

T
x
S

S

S

T

T

G

G

A

V

L

L

S

Q

D

P

A

L

K

D

G

G

L

F

C

G

Q

E

L

L

A

C

K

H

A

R

H

Y

D

K

C

C

L

L

S

L

I

L

V

V

D
|
D

A

S

V
|
V

T

A

S

S

L

L

G

G

V

T

E

P

L

L

R

Y

V

M

D

D

E

R

W

Q

G

G

I

I

D

D

A

I

I

L

Y

Y

S

S

G

G

S

S

Q

Q

K
|
K

C

A

L

L

S

N

C

A

V

P

P

P

G

G

L

T

S

S

P

L

V

I

S

S

F

F

S

S

P

D

A

K

A

A

V

K

E

K

K

K

L

M

K

Y

A

S

R

R

K

K

T

T

P

K

V

P

Q

F

S

S

W

F

F

Y

L

L

D

D

Q

I

S

K

L

W

V

L

M

A

G

N

Y

F

W

W

S

G

G

C

D

D

Q

D

G

Q

A

P

G

-

K

-

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

V

V

N

I

A

S

L

L

Y

Y

A

S

L

L

H

R

E

E

S

S

L

L

R

A

L

L

L

I

A

A

E

E

E

Q

G

G

L

L

E

E

N

N

A

S

W

W

A

R

R

Q

H

H

H

R

S

E

M

A

H

A

A

A

L

Y

L

L

R

H

D

G

G

R

L

L

E

Q

N

A

L

L

G

G

L

L

K

Q

-

L

F

F

V

V

V

K

E

D

E

P

G

A

S

L

R

R

L

L

P

P

Q

T

L

V

N

T

A

T

V

V

Y

A

I

V

P

P

E

A

G

G

V

Y

D

D

D

W

G

R

A

D

V

I

R

V

K

S

Q

Y

L

V

L

I

E

D

N

H

Y

F

N

D

L

I

E

E

I

I

G

M

A

G

G

G

L
|
L

G

G

A

P

L

S

A

T

G

G

K

K

A

V

W

L

R
|
R

I

I

G

G

L

L

M

L

G

G

F

C

G

N

A

A

R

T

R

R

E

E

N

N

V

V

A

D

L

R

C

V

L

T

R

E

A

A

L

L

E

R

E

A

V

A

L

L

R

Q

binding (aminooxy)acetic acid: P25 (= P20), G26 (= G21), L346 (= L346), R355 (= R355)
binding pyridoxal-5'-phosphate: S78 (≠ P73), G79 (= G74), H80 (≠ S75), W105 (≠ F100), S153 (≠ T151), D178 (= D176), V180 (= V178), K204 (= K202)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
40% identity, 98% coverage: 9:379/380 of query aligns to 14:380/385 of 2huuA

query
sites
2huuA

P

P

F

L

N

V

P

T

P

P

R

N

R

K

V

L

L

L

M

M

G

G

P
|
P

G

G

P

P

S

S

D

N

V

A

Y

P

P

Q

E

R

V

V

L

L

A

D

A

A

Q

M

A

S

R

R

P

P

T

I

I

L

G

G

H
|
H

L

L

D

H

P

P

L

E

F

T

V

L

G

K

M

I

M

M

D

D

E

D

L

I

K

K

S

E

L

G

I

V

Q

R

Y

Y

A

L

F

F

Q

Q

T

T

E

N

N

N

A

I

M

A

T

T

L

F

A

C

V

L

S

S

A

A

P

S

G

G

S

H

A

G

G

G

M

M

E

E

T

A

C

T

F

L

V

C

N

N

L

L

V

L

E

E

P

D

G

G

E

D

K

V

V

I

I

L

V

I

C

G

R

H

N

T

G

G

V

H

F

W

G

G

E

D

R

R

M

S

R

A

Q

D

N

M

V

A

E

T

R

R

V

Y

G

G

A

A

T

D

A

V

V

R

V

V

D

K

N

S

E

K

W

V

G

G

T

Q

P

S

V

L

S

S

L

L

S

D

D

E

V

I

E

R

A

D

A

A

L

L

K

L

A

I

H

H

-

K

P

P

D

S

A

V

K

L

F

F

L

L

A

-

F

-

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

A

V

L

L

S

Q

D

G

A

L

K

E

G

G

L

V

C

G

Q

A

L

L

A

C

K

H

A

Q

H

H

D

N

C

C

L

L

S

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

R

F

V

M

D

D

E

R

W

W

G

E

I

I

D

D

A

A

I

M

Y

Y

S

T

G

G

S

S

Q

Q

K
|
K

C

V

L

L

S

G

C

A

V

P

P

P

G

G

L

I

S

T

P

P

V

V

S

S

F

F

S

S

P

H

A

R

A

A

V

V

E

E

K

R

L

Y

K

K

A

R

R

R

K

N

T

T

P

K

V

V

Q

K

S

V

W

Y

F

Y

L

W

D

D

Q

M

S

S

L

L

V

V

M

G

G

D

Y

Y

W

W

S

-

G

-

D

-

Q

-

G

G

A

C

G

F

G

G

K

R

-

P

R

R

S

I

Y
|
Y

H

H

T

T

A

I

P

S

V

S

N

T

A

L

L

L

Y

Y

A

G

L

L

H

R

E

E

S

A

L

I

R

A

L

M

L

A

A

C

E

E

G

G

L

L

E

P

N

A

A

L

W

I

A

A

R

R

H

H

H

E

S

D

M

C

H

A

A

K

L

R

L

L

R

Y

D

R

G

G

L

L

E

Q

N

D

L

A

G

G

L

F

K

E

F

L

V

Y

V

A

E

D

E

P

G

K

S

D

R

R

L

L

P

S

Q

T

L

V

N

T

A

T

V

I

Y

K

I

V

P

P

E

Q

G

G

V

V

D

D

D

W

G

L

A

K

V

A

R

A

K

Q

Q

Y

L

A

L

M

E

K

N

T

Y

Y

N

L

L

V

E

E

I

I

G

S

A

G

G

G

L
|
L

G

G

A

P

L

T

A

A

G

G

K

Q

A

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

F

Q

G

N

A

A

R

T

E

E

N

R

V

V

A

D

L

R

C

V

L

L

R

Q

A

V

L

F

E

Q

E

E

V

A

L

V

binding 1-butanol: H45 (= H40), Y257 (= Y256)
binding alanine: P25 (= P20), S155 (≠ T151), K206 (= K202), L347 (= L346), R356 (= R355)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
40% identity, 98% coverage: 9:379/380 of query aligns to 14:380/385 of 2huiA

query
sites
2huiA

P

P

F

L

N

V

P

T

P

P

R

N

R

K

V

L

L

L

M

M

G

G

P
|
P

G

G

P

P

S

S

D

N

V

A

Y

P

P

Q

E

R

V

V

L

L

A

D

A

A

Q

M

A

S

R

R

P

P

T

I

I

L

G

G

H

H

L

L

D

H

P

P

L

E

F

T

V

L

G

K

M

I

M

M

D

D

E

D

L

I

K

K

S

E

L

G

I

V

Q

R

Y

Y

A

L

F

F

Q

Q

T

T

E

N

N

N

A

I

M

A

T

T

L

F

A

C

V

L

S

S

A

A

P

S

G

G

S

H

A

G

G

G

M

M

E

E

T

A

C

T

F

L

V

C

N

N

L

L

V

L

E

E

P

D

G

G

E

D

K

V

V

I

I

L

V

I

C

G

R

H

N

T

G

G

V

H

F

W

G

G

E

D

R

R

M

S

R

A

Q

D

N

M

V

A

E

T

R

R

V

Y

G

G

A

A

T

D

A

V

V

R

V

V

D

K

N

S

E

K

W

V

G

G

T

Q

P

S

V

L

S

S

L

L

S

D

D

E

V

I

E

R

A

D

A

A

L

L

K

L

A

I

H

H

-

K

P

P

D

S

A

V

K

L

F

F

L

L

A

-

F

-

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

A

V

L

L

S

Q

D

G

A

L

K

E

G

G

L

V

C

G

Q

A

L

L

A

C

K

H

A

Q

H

H

D

N

C

C

L

L

S

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

R

F

V

M

D

D

E

R

W

W

G

E

I

I

D

D

A

A

I

M

Y

Y

S

T

G

G

S

S

Q

Q

K
|
K

C

V

L

L

S

G

C

A

V

P

P

P

G

G

L

I

S

T

P

P

V

V

S

S

F

F

S

S

P

H

A

R

A

A

V

V

E

E

K

R

L

Y

K

K

A

R

R

R

K

N

T

T

P

K

V

V

Q

K

S

V

W

Y

F

Y

L

W

D

D

Q

M

S

S

L

L

V

V

M

G

G

D

Y

Y

W

W

S

-

G

-

D

-

Q

-

G

G

A

C

G

F

G

G

K

R

-

P

R

R

S

I

Y

Y

H

H

T

T

A

I

P

S

V

S

N

T

A

L

L

L

Y

Y

A

G

L

L

H

R

E

E

S

A

L

I

R

A

L

M

L

A

A

C

E

E

G

G

L

L

E

P

N

A

A

L

W

I

A

A

R

R

H

H

H

E

S

D

M

C

H

A

A

K

L

R

L

L

R

Y

D

R

G

G

L

L

E

Q

N

D

L

A

G

G

L

F

K

E

F

L

V

Y

V

A

E

D

E

P

G

K

S

D

R

R

L

L

P

S

Q

T

L

V

N

T

A

T

V

I

Y

K

I

V

P

P

E

Q

G

G

V

V

D

D

D

W

G

L

A

K

V

A

R

A

K

Q

Q

Y

L

A

L

M

E

K

N

T

Y

Y

N

L

L

V

E

E

I

I

G

S

A

G

G

G

L

L

G

G

A

P

L

T

A

A

G

G

K

Q

A

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

F

Q

G

N

A

A

R

T

E

E

N

R

V

V

A

D

L

R

C

V

L

L

R

Q

A

V

L

F

E

Q

E

E

V

A

L

V

binding glyoxylic acid: P25 (= P20), S155 (≠ T151), K206 (= K202), R356 (= R355)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
40% identity, 98% coverage: 9:379/380 of query aligns to 14:380/385 of 2hufA

query
sites
2hufA

P

P

F

L

N

V

P

T

P

P

R

N

R

K

V

L

L

L

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

A

Y

P

P

Q

E

R

V

V

L

L

A

D

A

A

Q

M

A

S

R

R

P

P

T

I

I

L

G

G

H
|
H

L

L

D

H

P

P

L

E

F

T

V

L

G

K

M

I

M

M

D

D

E

D

L

I

K

K

S

E

L

G

I

V

Q

R

Y

Y

A

L

F

F

Q

Q

T

T

E

N

N

N

A

I

M

A

T

T

L

F

A

C

V

L

S

S

A

A

P

S

G

G

S

H

A

G

G

G

M

M

E

E

T

A

C

T

F

L

V

C

N

N

L

L

V

L

E

E

P

D

G

G

E

D

K

V

V

I

I

L

V

I

C

G

R

H

N

T

G

G

V

H

F

W

G

G

E

D

R

R

M

S

R

A

Q

D

N

M

V

A

E

T

R

R

V

Y

G

G

A

A

T

D

A

V

V

R

V

V

D

K

N

S

E

K

W

V

G

G

T

Q

P

S

V

L

S

S

L

L

S

D

D

E

V

I

E

R

A

D

A

A

L

L

K

L

A

I

H

H

-

K

P

P

D

S

A

V

K

L

F

F

L

L

A

-

F

-

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

A

V

L

L

S

Q

D

G

A

L

K

E

G

G

L

V

C

G

Q

A

L

L

A

C

K

H

A

Q

H

H

D

N

C

C

L

L

S

L

I

I

V

V

D

D

A

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

R

F

V

M

D

D

E

R

W

W

G

E

I

I

D

D

A

A

I

M

Y

Y

S

T

G

G

S

S

Q

Q

K

K

C

V

L

L

S

G

C

A

V

P

P

P

G

G

L

I

S

T

P

P

V

V

S

S

F

F

S

S

P

H

A

R

A

A

V

V

E

E

K

R

L

Y

K

K

A

R

R

R

K

N

T

T

P

K

V

V

Q

K

S

V

W

Y

F

Y

L

W

D

D

Q

M

S

S

L

L

V

V

M

G

G

D

Y

Y

W

W

S

-

G

-

D

-

Q

-

G

G

A

C

G

F

G

G

K

R

-

P

R

R

S

I

Y
|
Y

H

H

T

T

A

I

P

S

V

S

N

T

A

L

L

L

Y

Y

A

G

L

L

H

R

E

E

S

A

L

I

R

A

L

M

L

A

A

C

E

E

G

G

L

L

E

P

N

A

A

L

W

I

A

A

R

R

H

H

H

E

S

D

M

C

H

A

A

K

L

R

L

L

R

Y

D

R

G

G

L

L

E

Q

N

D

L

A

G

G

L

F

K

E

F

L

V

Y

V

A

E

D

E

P

G

K

S

D

R

R

L

L

P

S

Q

T

L

V

N

T

A

T

V

I

Y

K

I

V

P

P

E

Q

G

G

V

V

D

D

D

W

G

L

A

K

V

A

R

A

K

Q

Q

Y

L

A

L

M

E

K

N

T

Y

Y

N

L

L

V

E

E

I

I

G

S

A

G

G

G

L

L

G

G

A

P

L

T

A

A

G

G

K

Q

A

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

F

Q

G

N

A

A

R

T

E

E

N

R

V

V

A

D

L

R

C

V

L

L

R

Q

A

V

L

F

E

Q

E

E

V

A

L

V

binding 1-butanol: H45 (= H40), S155 (≠ T151), Y257 (= Y256), R356 (= R355)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
40% identity, 98% coverage: 9:379/380 of query aligns to 14:380/393 of Q3LSM4

query
sites
Q3LSM4

P

P

F

L

N

V

P

T

P

P

R

N

R

K

V

L

L

L

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

A

Y

P

P

Q

E

R

V

V

L

L

A

D

A

A

Q

M

A

S

R

R

P

P

T

I

I

L

G

G