SitesBLAST

Comparing 6938073 FitnessBrowser__SB2B:6938073 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

2onjA Structure of the multidrug abc transporter sav1866 from s. Aureus in complex with amp-pnp (see paper)
34% identity, 79% coverage: 120:605/612 of query aligns to 96:577/578 of 2onjA

• binding phosphoaminophosphonic acid-adenylate ester: Y349 (= Y373), I356 (≠ V379), S376 (= S399), G377 (= G400), G378 (≠ A401), G379 (= G402), K380 (= K403), S381 (= S404), T382 (= T405), Q422 (= Q445), V476 (= V504), K477 (= K505), S479 (= S507), G480 (= G508), G481 (= G509), E503 (= E531), A507 (= A535), H534 (= H562)

2hydA Multidrug abc transporter sav1866 (see paper)
34% identity, 79% coverage: 120:605/612 of query aligns to 96:577/578 of 2hydA

• binding adenosine-5'-diphosphate: Y349 (= Y373), I356 (≠ V379), S376 (= S399), G377 (= G400), G378 (≠ A401), G379 (= G402), K380 (= K403), S381 (= S404), T382 (= T405), Y391 (= Y414), L463 (= L486), V476 (= V504), K477 (= K505), S479 (= S507), Q482 (= Q510)

Q9DC29 ATP-binding cassette sub-family B member 6; ABC-type heme transporter ABCB6; EC 7.6.2.5 from Mus musculus (Mouse) (see paper)
38% identity, 62% coverage: 226:607/612 of query aligns to 452:828/842 of Q9DC29

• G579 (≠ T353) mutation to E: Results in retention of the protein in the Golgi apparatus.

Sites not aligning to the query:

• 170 S→G: Results in retention of the protein in the Golgi apparatus.
• 356 L→P: Results in retention of the protein in the Golgi apparatus.

7dnyA Cryo-em structure of the human abcb6 (coproporphyrin iii-bound) (see paper)
36% identity, 68% coverage: 191:606/612 of query aligns to 158:570/571 of 7dnyA

• binding coproporphyrin III: W289 (= W320), Y293 (≠ E324), M296 (≠ S327)
• binding cholesterol hemisuccinate: L158 (≠ M191), I159 (≠ L192), L162 (= L195), C163 (= C196), L246 (≠ V277), L250 (≠ M281), L253 (≠ F284), L258 (≠ I289), Y261 (≠ L292), E265 (= E296)

Sites not aligning to the query:

• binding cholesterol hemisuccinate: 15, 16, 19, 20, 23, 27, 42, 47, 50, 155

Q9NP58 ATP-binding cassette sub-family B member 6; ABC-type heme transporter ABCB6; Mitochondrial ABC transporter 3; Mt-ABC transporter 3; P-glycoprotein-related protein; Ubiquitously-expressed mammalian ABC half transporter; EC 7.6.2.5 from Homo sapiens (Human) (see 11 papers)
36% identity, 68% coverage: 191:606/612 of query aligns to 415:827/842 of Q9NP58

• N447 (≠ D218) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-498; Q-677 and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-498; Q-677 and Q-775.
• A492 (≠ R266) to T: may be a modifier of disease severity in porphyria patients; increases expression; does not affect susbtrate binding; impairs ATP-binding; Loss of ATP-dependent coproporphyrin III transport; Highly decrease plasma membrane expression; dbSNP:rs147445258
• N498 (= N272) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-677 and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-677 and Q-775.
• T521 (= T295) to S: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs149363094
• V531 (≠ A305) mutation to A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.
• M542 (≠ G316) mutation to A: Loss of substrate-stimulate ATPase activity.
• W546 (= W320) mutation to A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.; mutation to F: Does not affect substrate-stimulate ATPase activity.; mutation to V: Loss of substrate-stimulate ATPase activity. Impairs protein expression.
• G579 (≠ T353) to E: in DUH3; the protein is retained in the Golgi apparatus. Does not affect subcellular location in early melanosome and lysosome. Does not rescue the normal amyloid fibril formation and normal maturation of pigmented melanosomes. Does not influence trafficking of melanosomal proteins.; dbSNP:rs397514758
• G588 (= G362) to S: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs145526996
• Y599 (= Y373) binding
• 623:634 (vs. 397:408, 67% identical) binding
• K629 (= K403) mutation to A: Abolishes ATP hydrolysis. Abolishes coproporphyrin III transport.; mutation to M: Does not affect subcellular location in early melanosome and lysosome. Does not rescue the normal amyloid fibril formation and normal maturation of pigmented melanosomes. Does not influence trafficking of melanosomal proteins. Fails to rescue vacuolar sequestration of cadmium in Schizosaccharomyces pombe and Caenorhabditis elegans strains defective for HMT-1. Fails to rescue the cadmium tolerance in Schizosaccharomyces pombe and Caenorhabditis elegans strains defective for HMT-1. Does not rescue vacuolar cadmium levels in hmt-1 mutant S.pombe.
• N677 (≠ H451) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-498; and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-498; and Q-775.
• A681 (≠ R455) to T: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs142421126
• N775 (≠ G554) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-498 and Q-677. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-498 and Q-677.
• L811 (= L590) to V: in MCOPCB7; hypomorphic mutation; dbSNP:rs387906910

Sites not aligning to the query:

• 1:205 Required for the lysosomal targeting
• 1:236 Required for ATPase activity
• 6 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Loss of N-glycosylation; when associated with Q-447; Q-498; Q-677 and Q-775. Does not affect subtrate binding.
• 8 modified: Disulfide link with 26; C→G: Loss of N-glycosylation.; C→S: Does not affect subtrate binding. Does not affect N-glycosylation. Impairs endoplasmic reticulum exit. Impairs endoplasmic reticulum exit; when associated with C-8. Increases ABCB6 proteasomal degradation. Affects protein stability. Does not affect migration in the presence of DTT; when associated with A-50 and A-120.
• 26 modified: Disulfide link with 8; C→A: Decreases protein expression. Affects protein stability. Loss of ability to stimulate porphyrin synthesis.; C→S: Decreases protein expression. Impairs endoplasmic reticulum exit; when associated with C-8. Affects protein stability.
• 50 C→A: Increases migration in the absence of DTT; when associated with A-120. Reduces migration in with the presence of DTT; when associated with A-120.
• 57 A → T: in MCOPCB7; unknown pathological significance; hypomorphic mutation; dbSNP:rs387906911
• 69 R → G: in a breast cancer sample; somatic mutation
• 120 C→A: Increases migration in the absence of DTT; when associated with A-50. Reduces migration in with the presence of DTT; when associated with A-50.
• 170 S → G: in DUH3; the protein is retained in the Golgi apparatus; dbSNP:rs397514757
• 192 R → Q: decrease expression; does not affect susbtrate binding; does not affect ATP-binding; loss of plasma membrane expression; dbSNP:rs150221689
• 276 R → W: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs57467915
• 286 Y→A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.
• 356 L → P: in DUH3; the protein is retained in the Golgi apparatus; dbSNP:rs397514756

7eklA Mitochondrial outer membrane protein (see paper)
35% identity, 68% coverage: 191:606/612 of query aligns to 178:590/590 of 7eklA

• binding adenosine-5'-triphosphate: Y362 (= Y373), R366 (≠ S377), S388 (= S399), G389 (= G400), A390 (= A401), G391 (= G402), K392 (= K403), S393 (= S404), T394 (= T405), Q434 (= Q445), F475 (≠ L486), K489 (= K505), L490 (= L506), S491 (= S507), G492 (= G508), G493 (= G509), E494 (≠ Q510), H546 (= H562)
• binding magnesium ion: S393 (= S404), Q434 (= Q445)

7dnzA Cryo-em structure of the human abcb6 (hemin and gsh-bound) (see paper)
36% identity, 68% coverage: 191:604/612 of query aligns to 180:590/591 of 7dnzA

• binding glutathione: R204 (≠ K210), R215 (≠ T224), Y241 (≠ A250), L262 (= L271), N263 (= N272), Q266 (≠ V275), N310 (≠ Q319), W311 (= W320), G313 (≠ M322), T314 (≠ W323), R317 (≠ S326)
• binding protoporphyrin ix containing fe: W311 (= W320), Y315 (≠ E324)
• binding cholesterol hemisuccinate: L180 (≠ M191), I181 (≠ L192), L184 (= L195), L280 (≠ I289), E287 (= E296)

Sites not aligning to the query:

• binding glutathione: 111, 135, 139
• binding cholesterol hemisuccinate: 37, 42, 64, 69, 72, 165, 177

7n5aA Structure of atatm3 in the closed conformation (see paper)
37% identity, 69% coverage: 185:605/612 of query aligns to 177:588/589 of 7n5aA

• binding adenosine-5'-diphosphate: Y361 (= Y373), G388 (= G400), S389 (≠ A401), G390 (= G402), K391 (= K403), S392 (= S404), T393 (= T405), K488 (= K505), S490 (= S507), E493 (≠ Q510)

7n59A Structure of atatm3 in the inward-facing conformation with gssg bound (see paper)
37% identity, 69% coverage: 185:605/612 of query aligns to 178:589/590 of 7n59A

• binding oxidized glutathione disulfide: R198 (= R208), R202 (= R211), L257 (≠ V268), Q264 (≠ V275), N308 (= N315), F309 (≠ G316), S312 (= S326)

Q9LVM1 ABC transporter B family member 25, mitochondrial; ABC transporter ABCB.25; AtABCB25; ABC transporter of the mitochondrion 3; AtATM3; Iron-sulfur clusters transporter ATM3; Protein STARIK 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 69% coverage: 185:605/612 of query aligns to 304:715/728 of Q9LVM1

• R612 (= R502) mutation to K: Resistant to sirtinol and reduced chlorophyll content.

7metA A. Baumannii msba in complex with tbt1 decoupler (see paper)
34% identity, 83% coverage: 98:604/612 of query aligns to 56:563/564 of 7metA