SitesBLAST

Q9FX01 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; At3BETAHSD/D1; 4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1; Reticulon-like protein B24; AtRTNLB24; Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating; EC 1.1.1.418 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 58% coverage: 4:181/307 of query aligns to 13:203/439 of Q9FX01

query
sites
Q9FX01

L

V

I

V

T

T

G

G

S

R

G

G

F

F

V

A

G

A

R

R

Y

H

L

L

-

V

Q

E

G

M

Y

L

F

V

N

R

S

Y

D

Q

E

M

Y

F

D

H

V

V

A

R

I

I

T

A

S
x
D

R

L

C

A

K

P

S

A

N

I

I

V

-

L

-

N

-

P

-

H

-

E

-

T

-

G

-

I

-

L

-

G

-

E

-

A

-

I

-

R

S

S

D

G

F

R

P

V

V

Q

Y

Y

T

V

I

S

P

A

A
x
D

I

L

D

R

Q

N

H

K

T

T

D

Q

W

V

K

V

H

K

A

G

L

F

K

Q

N

G

V

A

D

E

V

V

V

F

H

H

L

M

A

A

S

A

R

P

A

D

H

S

S

S

S

I

D

N

N

N

H

H

S

Q

E

L

A

Q

A

-

K

-

E

-

D

-

F

-

T

Y

K

S

T

V

N

N

V

V

L

Q

G

G

L

T

S

T

K

N

L

V

L

I

D

D

D

A

C

C

I

I

F

E

L

V

G

G

V

V

K

K

R

R

I

L

I

I

Y

Y

L
x
T

S

S

S
|
S

I

P

K
x
S

A

V

L

V

G

F

E

D

S

G

T

V

D

H

G

G

R

T

D

L

A

N

F

A

S

D

E

E

S

S

D

L

K

P

Y

Y

N

P

P

P

A

K

-

H

-

N

D

D

Y

S

Y
|
Y

G

S

I

A

T

T

K
|
K

A

A

R

E

A

G

E

E

Q

A

L

L

V

I

V

L

E

K

K

A

C

N

K

G

H

R

S

S

G

G

L

L

D

L

Y

T

T

C

I

C

I

I

R

R

P

P

P

S

L

S

V

I

Y

F

G

G

K

P

S

G

A

D

K

K

A

L

N

M

L

V

Q

P

S

S

I

L

D41 (≠ S31) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
D72 (≠ A47) Essential for the 3betaHSD/D activity; mutation D->A,V: Lost activity.
T131 (≠ L111) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
S133 (= S113) mutation to A: Reduced activity with slower catalysis and lower substrate binding.
S135 (≠ K115) mutation to A: Normal activity, but slower catalysis with 4alpha-carboxysterol as substrate.; mutation to T: Altered activity due to reduced affinity and catalysis and leading to lower amount of C4-demethylated sterols and higher quantities of 4,4-dimethylated sterol intermediates, and a higher ratio of 4,4-dimethylsterols to 4alpha-methylsterols.; mutation to Y: Altered activity leading to an exclusive production of C4-methylated sterols and 4alpha-carboxy-3beta-hydroxy sterols.
Y161 (= Y139) Essential for the 3betaHSD/D activity; mutation to F: Lost activity.
K165 (= K143) Essential for the 3betaHSD/D activity; mutation to I: Lost activity.

Sites not aligning to the query:

320 R→I: Normal activity.
328 Essential for the 3betaHSD/D activity; R→I: Lost activity.

5u9cA 1.9 angstrom resolution crystal structure of dtdp-4-dehydrorhamnose reductase from yersinia enterocolitica
27% identity, 86% coverage: 2:265/307 of query aligns to 4:251/288 of 5u9cA

query
sites
5u9cA

K

K

V

I

L

L

I

I

T

T

G

G

S

T

G

G

F

M

V

L

G

G

R

A

Y

Y

L

V

Q

T

G

S

Y

A

F

L

N

K

S

D

D

T

E

D

Y

Y

D

N

V

V

A

I

I

V

T

T

S

E

R

R

C

N

K

T

S

L

N

N

I

L

S

S

D

-

F

-

P

-

V

-

Y

-

T

-

I

V

P

P

-

E

A

A

I

I

D

F

Q

S

H

Y

T

I

D

-

W

-

K

-

H

-

A

T

L

A

K

E

N

K

V

P

D

D

V

V

V

I

V

L

H

H

L

F

A

A

S

A

R

E

A

T

H

D

S

V

S

D

D

L

N

C

H

E

S

R

E

E

A

P

A

A

K

R

E

A

D

G

F

I

T

-

K

-

T

Y

N

N

V

H

L

L

G

A

L

T

S

E

K

Q

L

I

L

A

D

Q

D

A

C

A

I

K

F

F

L

C

G

G

V

A

K

-

R

W

I

L

Y

Y

L

L

S

S

I

S

K

N

A

V

L

F

G

G

E

-

S

-

T

G

D

E

G

G

R

K

D

L

A

S

F

Y

S

N

E

E

S

L

D

D

K

I

Y

P

N

L

P

P

A

M

D

N

Y

Y

G

G

I

R

T

S

K

K

A

L

R

I

A

G

E

E

Q

S

L

S

V

V

V

R

E

N

K

A

C

C

K

T

H

N

S

-

G

-

L

-

D

N

Y

H

T

L

I

I

R

R

P

A

P

G

L

W

V

M

Y

I

G

G

K

G

S

G

A

P

K

D

A

K

N

D

-

H

-

K

-
x
F

-

V

-

G

-

K

-

I

L

I

Q

Q

S

Q

I

I

A

K

R

A

G

G

L

-

S

S

Y

T

S

S

L

I

P
x
K

F

-

P

-

Y

-

F

A

T

V

S

S

S

D

N

R

R

L

R

G

S

S

L

I

I

T

S

S

V

A

H

M

S

Q

L

L

C

C

R

N

F

F

I

I

V

I

A

W

V

A

A

I

N

N

D

K

P

R

N

H

T

T

N

G

N

T

Q

-

I

-

Y

L

N

H

V

F

A

A

D

S

N

S

Y

G

V

T

L

I

S

S

T

R

K

F

D

D

I

I

F

A

S

C

L

A

I

I

K

G

S

D

C

L

S

L

N

N

S

F

K

K

S

G

F

D

I

I

F

I

P

P

I

V

P

H

K

S

F

S

I

V

F
|
F

T

P

F

L

L

S

A

A

binding beta-D-fructopyranose: F165 (vs. gap), K181 (≠ P190), F247 (= F261)

Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 55% coverage: 3:171/307 of query aligns to 11:193/667 of Q9LPG6

query
sites
Q9LPG6

V

I

L

L

I

I

T

T

G

G

G

A

S

A

G
|
G

F

F

V

I

G

A

R

S

Y

H

L

V

Q

A

G

N

Y

R

F

L

-

I

-

R

N

N

S

Y

D

P

E

D

Y

Y

D
x
K

V

I

A

V

I

V

T

L

S

D

R

K

C

L

K

D

-

Y

-

C

S

S

N

D

I

L

S

K

D

N

F

L

-

D

P

P

V

S

Y

F

T

S

I

S

P

P

-

N

-

F

-

K

-

F

-

V

-

K

-

G

-

D

-

I

A

A

I

S

D

D

Q

D

H

L

T

V

D

N

W

Y

K

L

H

L

A

I

L

T

K

E

N

N

V

I

D

D

V

T

V

I

V

M

H

H

L

F

A

A

S

A

R

Q

A

T

H

H

S

V

S
x
D

D

N

N

S

H

F

S

G

E

N

A

S

A

F

K

-

E

-

D

E

F

F

T

T

K

K

T

N

N

N

V

I

L

Y

G

G

L

T

S

H

K

V

L

L

D

E

D

A

C

C

I

K

F

V

L

T

G

G

-

Q

V

I

K

R

R

R

I

F

I

I

Y

H

L

V

S

S

S

T

I

D

K

E

A

V

L

Y

G

G

E

E

S

T

-

D

T

E

D

D

G

A

R

A

D

V

A

G

F

N

S

H

E

E

S

A

D

S

K

Q

Y

L

N

L

P

P

A

T

D

N

Y

P

Y

Y

G

S

I

A

T

T

K
|
K

A

A

R

G

A

A

E

E

Q

M

L

L

V

V

V

M

E

A

K

Y

C

G

K

R

H

S

S

Y

G

G

L

L

D

P

Y

V

T

I

I

T

R

R

P

G

P

N

L

N

V

V

Y

Y

G
|
G

G18 (= G10) mutation to A: Abolishes dehydratase activity.
K36 (≠ D26) mutation to A: Reduces dehydratase activity.
D96 (≠ S74) mutation to N: In mum4-1; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
K165 (= K143) mutation to A: Abolishes dehydratase activity.
G193 (= G171) mutation to R: In mum4-2; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.

Sites not aligning to the query:

392 G→A: No effect on dehydratase activity.
413 K→A: No effect on dehydratase activity.
518 K→A: No effect on dehydratase activity.

2hunA Crystal structure of hypothetical protein ph0414 from pyrococcus horikoshii ot3
28% identity, 75% coverage: 1:229/307 of query aligns to 2:238/329 of 2hunA

query
sites
2hunA

M

M

K

K

V

L

L

L

I

V

T

T

G
|
G

G

G

S

M

G
|
G

F
|
F

V
x
I

G

G

-

S

-

N

-

F

-

I

R

R

Y

Y

L

I

-

L

-

E

-

K

-

H

-

P

-

D

Q

W

G

E

Y

V

F

I

N

N

S

I

D
|
D

E
x
K

Y

L

D

G

V

Y

A

G

I
x
S

T

N

S

P

R

A

C

N

K

L

S

K

N

D

I

L

S

E

D

D

F

D

P

P

V

R

Y

Y

T

T

I

F

P

V

A

K

I

G

D
|
D

-
x
V

-

A

Q

D

H

Y

T

E

D

L

W

V

K

K

H

E

A

L

L

V

K

R

N

K

V

V

D

D

V

G

V

V

H

H

L
|
L

A
|
A

S
x
A

R

E

A

S

H

H

S

V

S

-

D

-

N

D

H

R

S

S

E

I

A

S

K

P

E

E

D

I

F

F

T

L

K

H

T
x
S

N

N

V

V

L

I

G

G

L

T

S

Y

K

T

L

L

D

E

D

S

C

I

I

R

F

R

L

E

G

N

V

P

K

E

-

V

R

R

I

F

I

V

Y

H

L

V

S

S

S
x
T

I
x
D

K
x
E

A

V

L

Y

G

G

E

D

S

I

T

L

D

K

G

G

R

-

D

-

A

S

F

F

S

T

E

E

S

N

D

D

K

R

Y

L

N

M

P

P

A

S

D

S

Y

P

Y
|
Y

G

S

I

A

T

T

K
|
K

A

A

R

A

A

S

E

D

Q

M

L

L

V

V

V

L

E

G

K

W

C

T

K

R

H

T

S

Y

G

N

L

L

D

N

Y

A

T

S

I

I

I

T

R

R

P
x
C

P
x
T

L
x
N

V
x
N

Y

Y

G

G

K

P

-

Y

-

Q

-

F

S

P

A

E

K

K

A

L

N

I

L

P

Q

K

S

T

I

I

A

I

R

R

G

A

L

-

S

S

Y

L

S

G

L

L

P

K

F

I

P

P

Y

I

F

Y

T

G

S

T

S

V

N

-

R

-

R

R

S

D

L

W

I

L

S

Y

V

V

H

E

S

D

L

H

C

V

R

R

F

A

I

I

V

E

A

L

V

V

A

L

N

L

D

K

P

-

N

G

T

E

N

S

N

R

Q

E

I

I

Y

Y

N

N

V

I

A

S

active site: T125 (≠ S113), D126 (≠ I114), E127 (≠ K115), Y149 (= Y139), K153 (= K143)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ V12), D34 (= D23), K35 (≠ E24), S40 (≠ I29), D60 (= D49), V61 (vs. gap), L80 (= L67), A81 (= A68), A82 (≠ S69), S99 (≠ T88), T125 (≠ S113), K153 (= K143), C176 (≠ P166), T177 (≠ P167), N178 (≠ L168), N179 (≠ V169)

6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
25% identity, 79% coverage: 1:243/307 of query aligns to 1:257/313 of 6bwlA

query
sites
6bwlA

M

M

K

K

V

I

L

L

I

V

T

T

G
|
G

G

G

S

A

G
|
G

F
|
F

V
x
I

G

G

R

R

Y

W

L

V

Q

V

G

K

Y

R

F

L

N

L

S

Q

D

D

E

K

Y

H

D

E

V

V

A

W

I

I

-

L

-
x
D

-
x
N

-
x
L

T

A

S
x
N

R
x
S

C

T

K

T

S

A

N

N

I

I

S

T

D

E

F

F

P

A

-

H

-

D

V

L

Y

N

T

L

I

K

P

Q

A

C

I

I

D

Q

Q

G

H
x
D

T
x
I

D

K

W

D

K

K

H

K

A

L

L

V

K

A

N

Q

V

L

-

F

-

E

-

N

-

S

-

F

D

D

V

L

V

C

V

Y

H

H

L
|
L

A
|
A

S
x
A

R

S

A
x
I

H

N

S

V

S

Q

D

D

N

S

H

I

S

D

E

D

A

-

A

A

K

R

E

A

D

T

F

F

T

-

K

E

T

N

N

D

V

T

L

I

G

G

L

T

S

F

K

N

L

L

D

E

D

Q

C

C

I

L

F

N

L

Y

G

D

V

V

K

K

R

-

I

M

I

V

Y

F

L
x
M

S

S

S
x
T

I
x
C

K
x
M

A

V

L

Y

G

D

E

K

S

A

T

T

D

N

G

I

R

Q

D

-

A

G

F

I

S

S

E

E

S

L

D

D

K

P

Y

I

N

K

P

P

A

A

D

S

Y

P

Y
|
Y

G

A

I

G

T

S

K
|
K

A

I

R

A

A

A

E

E

Q

N

L

M

V

V

V

L

E

S

K

Y

C

Y

K

Y

H

A

S

Y

G

K

L

L

D

P

Y

V

T

V

I

V

I

I

R

R

P

P

P

F

L
x
N

V
x
T

Y

Y

G

G

K

P

S

F

A

Q

K

K

A

T

N

G

L

G

Q

E

S

G

-
x
G

-
x
V

I

V

A

A

R

I

G

F

L

I

S

N

Y

N

S

K

L

L

-

D

-

N

-

V

P

P

F

L

P
x
N

-
x
I

Y
|
Y

F

G

T

D

S

G

S

K

N
x
Q

R

T

R
|
R

S

D

L

L

I

L

S

Y

V

V

H

E

S

D

L

C

C

A

R

D

F

F

I

V

V

V

A

A

V

A

A

G

N

Y

D

S

P

A

N

K

T

A

N

N

N

G

Q

H

I

I

Y

I

N

N

V

A

A

G

D

T

N

G

Y

Q

V

D

L

I

S

S

T
x
I

K

N

D

K

I

L

F

A

S

E

L

L

I

I

active site: T122 (≠ S113), C123 (≠ I114), M124 (≠ K115), Y147 (= Y139), K151 (= K143)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (vs. gap), N32 (vs. gap), L33 (vs. gap), N35 (≠ S31), S36 (≠ R32), D57 (≠ H51), I58 (≠ T52), L79 (= L67), A80 (= A68), A81 (≠ S69), I83 (≠ A71), M120 (≠ L111), K151 (= K143), N176 (≠ L168), T177 (≠ V169)
binding uridine-5'-diphosphate: N176 (≠ L168), G189 (vs. gap), V190 (vs. gap), N205 (≠ P192), I206 (vs. gap), Y207 (= Y193), Q212 (≠ N198), R214 (= R200), I250 (≠ T236)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 275

H1ZZB0 Aurachin B dehydrogenase; EC 1.1.1.394 from Stigmatella aurantiaca (see paper)
22% identity, 84% coverage: 1:257/307 of query aligns to 1:255/334 of H1ZZB0

query
sites
H1ZZB0

M

M

K

R

V

T

L

F

I

V

T

T

G

G

S

S

G

G

F

Y

V

L

G

G

R

R

Y

N

L

L

Q

L

G

S

Y

A

F

L

N

V

S

A

D

R

E

G

Y

I

D

S

V

V

A

R

I

A

T

L

S

V

R

R

C

S

K

E

-

E

-

A

S

Q

N

K

I

V

S

Q

D

A

F

L

P

G

V

A

Y

Q

T

P

I

I

P

L

A

G

I

T

D

L

Q

E

H

H

T

R

D

E

-

T

W

L

K

K

H

E

A

G

L

M

K

A

N

G

V

C

D

D

V

V

V

L

V

F

H

H

L

A

A

A

S

A

R

L

A

-

H

-

S

-

D

-

N

T

H

S

S

A

E

R

A

A

A

T

K

D

E

A

D

E

F

F

T

H

K

R

T

A

N

N

V

V

L

L

G

G

L

T

S

E

K

T

L

V

L

L

D

A

C

A

I

R

F

D

L

A

G

R

V

I

K

Q

R

R

I

M

I

V

Y

H

L

V

S
|
S

S

T

I

E

K

A

A

V

L

L

G

-

E

-

S

-

T

A

D

D

G

G

R

R

D

P

A

L

F

L

S

Q

E

V

S

D

D

E

K

S

Y

H

-

P

-

L

-

P

-

K

N

R

P

P

A

F

D

A

Y

G

Y
|
Y

G

P

I

A

T

T

K

K

A

A

R

Q

A

A

E

E

Q

Q

L

L

V

V

V

L

E

Q

K

-

C

A

K

N

H

G

S

P

G

G

L

F

D

T

Y

T

T

V

I

V

R

R

P

P

P

R

L

F

V

I

Y

W

G

G

K

A

S

D

A

D

K

T

A

A

N

F

L

L

Q

P

S

Q

I

L

-

I

-

D

-

A

-

I

-

R

A

T

R

K

G

R

L

F

S

R

Y

W

S

V

L

D

P

G

F

G

P

R

Y

Y

F

L

T

T

S

S

S

T

N

-

R

-

S

-

L

-

I

C

S

H

V

V

H

A

S

N

L

V

C

C

R

E

F

G

I

M

V

L

A

-

V

L

A

A

N

A

D

E

P

R

N

G

T

P

N

G

Q

E

I

V

Y

Y

N

F

V

L

A

T

D

D

N

G

Y

A

V

P

L

V

S

E

T

L

K

R

D

S

I

F

F

L

S

T

L

L

I

L

K

L

S

E

C

T

S

Q

N

G

S

I

K

K

S

A

F

E

I

V

F

G

P

N

I

I

P

P

S111 (= S112) mutation to A: Does not produce aurachin B.
Y139 (= Y139) mutation to F: Does not produce aurachin B.

6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
25% identity, 78% coverage: 3:242/307 of query aligns to 3:240/299 of 6kvcA

query
sites
6kvcA

V

V

L

L

I

V

T

T

G
|
G

G

A

S

A

G
|
G

F
|
F

V
x
I

G

G

R

S

Y

H

L

L

Q

V

G

T

Y

E

F

L

-

R

N

N

S

S

D

G

E

R

Y

N

D

V

V

V

A

A

I

V

T
x
D

S
x
R

R
|
R

C

-

K

-

S

-

N

-

I

-

S

-

D

P

F

L

P

P

V

T

Y

G

T

S

I

L

P

R

A

E

I

I

D

R

-

G

-
x
D

-
x
L

Q

N

H

S

T

L

D

N

W

L

K

V

H

D

A

C

L

L

K

K

N

N

V

I

D

S

V

T

V

V

V

F

H

H

L
|
L

A
|
A

S

-

R

-

A
|
A

H

L

S
x
P

S

G

D

V

N
x
R

H

P

S

S

E

W

A

T

A

Q

K

F

E

P

D

E

F

Y

T

L

K

R

T

C

N

N

V

V

L

L

G

A

L

T

S

Q

K

R

L

L

L

M

D

E

D

A

C

C

I

V

F

Q

L

A

G

G

V

V

K

E

R

R

I

V

Y

V

L
x
A

S

S

S
|
S

I
x
S

K
x
S

A

V

L

Y

G

G

E

-

S

G

T

A

D

D

G

G

R

-

D

-

A

V

F

M

S

S

E

E

S

D

D

D

K

L

Y

P

N

R

P

P

A

L

D

S

Y

P

Y
|
Y

G

G

I

V

T

T

K
|
K

A

L

R

A

A

A

E

E

Q

R

L

L

V

A

V

L

E

A

K

F

C

A

K

A

H

R

S

G

G

D

L

A

D

E

Y

L

T

S

I

V

-

G

-

A

I

L

R

R

P
x
F

P

F

L
x
T

V
|
V

Y

Y

G

G

K

P

S

G

A

Q

K
x
R

A

P

N

D

L
x
M

-
x
F

-

I

Q

S

S
x
R

I

L

A

I

R

R

G

A

L

T

S

L

Y

R

S

G

L

E

P

P

F

V

P
x
E

-
x
I

Y
|
Y

F

G

T

D

S

G

S

T

N
x
Q

R

L

R
|
R

S

D

L

F

I

T

S

H

V

V

H

S

S

D

L

V

C

V

R

R

F

A

I

L

V

M

A

L

V

T

A

A

N

S

D

V

P

R

N

D

T

R

N

G

N

S

Q

A

I

V

Y

L

N

N

V

I

A

G

D

T

N

G

Y

S

V

A

L

V

S

S

T

V

K

N

D

E

I

V

F

V

S

S

L

M

active site: S109 (= S113), S110 (≠ I114), S111 (≠ K115), Y132 (= Y139), K136 (= K143)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ T30), R32 (≠ S31), R33 (= R32), D46 (vs. gap), L47 (vs. gap), L65 (= L67), A66 (= A68), A67 (= A71), P69 (≠ S73), A107 (≠ L111), S109 (= S113), K136 (= K143), F161 (≠ P166), T163 (≠ L168), V164 (= V169), R170 (≠ K175), M173 (≠ L178)
binding uridine-5'-diphosphate-glucose: P69 (≠ S73), R72 (≠ N76), S109 (= S113), S110 (≠ I114), Y132 (= Y139), T163 (≠ L168), M173 (≠ L178), F174 (vs. gap), R177 (≠ S180), E189 (≠ P192), I190 (vs. gap), Y191 (= Y193), Q196 (≠ N198), R198 (= R200)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucose: 260

6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
25% identity, 78% coverage: 3:242/307 of query aligns to 3:241/299 of 6kv9A

query
sites
6kv9A

V

V

L

L

I

V

T

T

G
|
G

G

A

S

A

G
|
G

F
|
F

V
x
I

G

G

R

S

Y

H

L

L

Q

V

G

T

Y

E

F

L

-

R

N

N

S

S

D

G

E

R

Y

N

D

V

V

V

A

A

I

V

T
x
D

S
x
R

R
|
R

C

P

K

L

S

P

N

D

I

T

S

G

D

S

F

L

P

-

V

-

Y

R

T

E

I

I

P

R

A

G

I
x
D

D
x
L

Q

N

H

S

T

L

D

N

W

L

K

V

H

D

A

C

L

L

K

K

N

N

V

I

D

S

V

T

V

V

V

F

H

H

L
|
L

A
|
A

S

-

R

-

A
|
A

H

L

S
x
P

S

G

D

V

N
x
R

H

P

S

S

E

W

A

T

A

Q

K

F

E

P

D

E

F

Y

T

L

K

R

T
x
C

N

N

V

V

L

L

G

A

L

T

S

Q

K

R

L

L

L

M

D

E

D

A

C

C

I

V

F

Q

L

A

G

G

V

V

K

E

R

R

I

V

Y

V

L
x
A

S
|
S

I
x
S

K
x
S

A

V

L

Y

G

G

E

-

S

G

T

A

D

D

G

G

R

-

D

-

A

V

F

M

S

S

E

E

S

D

D

D

K

L

Y

P

N

R

P

P

A

L

D

S

Y

P

Y
|
Y

G

G

I

V

T

T

K
|
K

A

L

R

A

A

A

E

E

Q

R

L

L

V

A

V

L

E

A

K

F

C

A

K

A

H

R

S

G

G

D

L

A

D

E

Y

L

T

S

I

V

-

G

-

A

I

L

R

R

P
x
F

P

F

L
x
T

V
|
V

Y

Y

G

G

K

P

S

G

A

Q

K
x
R

A

P

N

D

L
x
M

-
x
F

-

I

Q

S

S
x
R

I

L

A

I

R

R

G

A

L

T

S

L

Y

R

S

G

L

E

P

P

F

V

P
x
E

-
x
I

Y
|
Y

F

G

T

D

S

G

S

T

N
x
Q

R

L

R
|
R

S

D

L

F

I

T

S

H

V

V

H

S

S

D

L

V

C

V

R

R

F

A

I

L

V

M

A

L

V

T

A

A

N

S

D

V

P

R

N

D

T

R

N

G

N

S

Q

A

I

V

Y

L

N

N

V

I

A

G

D

T

N

G

Y

S

V

A

L

V

S

S

T
x
V

K

N

D

E

I

V

F

V

S

S

L

M

active site: S110 (= S113), S111 (≠ I114), S112 (≠ K115), Y133 (= Y139), K137 (= K143)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ T30), R32 (≠ S31), R33 (= R32), D47 (≠ I48), L48 (≠ D49), L66 (= L67), A67 (= A68), A68 (= A71), P70 (≠ S73), C85 (≠ T88), A108 (≠ L111), S109 (= S112), K137 (= K143), F162 (≠ P166), T164 (≠ L168), V165 (= V169), R171 (≠ K175), M174 (≠ L178)
binding uridine-5'-diphosphate-glucuronic acid: P70 (≠ S73), R73 (≠ N76), S110 (= S113), S111 (≠ I114), Y133 (= Y139), T164 (≠ L168), R171 (≠ K175), M174 (≠ L178), F175 (vs. gap), R178 (≠ S180), E190 (≠ P192), I191 (vs. gap), Y192 (= Y193), Q197 (≠ N198), R199 (= R200), V235 (≠ T236)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucuronic acid: 261

6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
22% identity, 84% coverage: 1:257/307 of query aligns to 1:274/314 of 6zljA

query
sites
6zljA

M

M

K

K

V

I

L

L

I

V

T

T

G
|
G

G

A

S

A

G
|
G

F
|
F

V
x
I

G

G

R

S

Y

H

L

L

-

C

Q

Q

G

A

Y

L

F

L

N

K

S

N

D

S

E

A

Y

Y

D

H

-

V

V

V

A

G

I

I

T
x
D

S
x
H

R
x
F

C
x
I

K

G

S

P

N

T

I

P

S

A

D

T

F

L

P
x
K

V

T

Y

G

T

N

I

I

P

Q

A

S

I

L

D

E

-

L

-

N

-

S

-

R

-

F

-

Q

-

F

-

I

-

R

-

E

-
x
D

-
x
I

Q

L

H

N

T

T

D

D

W

L

K

S

H

K

A

L

L

L

K

Q

N

D

V

I

D

D

V

V

V

Y

H

H

L
|
L

A
|
A

S
x
A

R

I

A
x
P

H

G

S

V

S
x
R

D

T

N

S

H

W

S

G

E

K

A

D

A

F

K

-

E

Q

D

P

F

Y

T

V

K

T

T

N

N

N

V

I

L

M

G

V

L

T

S

Q

K

Q

L

L

D

E

D

A

C

C

I

K

F

H

L

I

G

K

V

L

K

D

R

K

I

F

I

I

Y

H

L
x
I

S

S

S
x
T

I
x
S

K
x
S

A

V

L

Y

G

G

E

E

S

K

T

S

D

-

G

-

R

-

D

G

A

A

F

V

S

S

E

E

S

D

D

L

K

L

Y

P

N

I

P

P

A

L

D

S

Y

P

Y
x
F

G

G

I

V

T

T

K
|
K

A

L

R

S

A

G

E

E

Q

H

L

L

V

C

V

H

E

V

K

Y

C

H

K

K

H

N

S

F

G

H

L

I

D

P

Y

I

T

V

I

I

I

L

R

R

P
x
Y

P
x
F

L
x
T

V
|
V

Y

Y

G

G

K

P

S

R

A

Q

K
x
R

A

P

N

D

L
x
M

-
x
A

-

F

Q

H

S
x
R

I

L

A

I

R

K

G

Q

L

M

S

L

Y

E

S

D

L

K

P

P

F

L

P
x
T

Y

I

F
|
F

T

G

S

D

S

G

N

T

R
x
Q

-

T

R
|
R

S

D

L

F

I

T

S

Y

V

I

H

D

S

D

L

C

C

I

R

R

F

G

I

T

V

V

-

A

A

A

V

L

A

E

N

T

D

K

P

K

N

N

T

I

N

I

N

G

Q

E

I

V

Y

I

N

N

V

I

A

G

D

G

N

K

Y

E

V

Q

L

A

S

S

T
x
I

K

L

D

D

I

I

F

I

S

S

L

M

I

L

K

E

S

K

C

I

S

S

N

G

-

K

-

S

-

A

S

T

K

K

S

N

F

F

I

L

F

K

P

S

I

V

P

P

active site: T126 (≠ S113), S127 (≠ I114), S128 (≠ K115), F149 (≠ Y139), K153 (= K143)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D32 (≠ T30), H33 (≠ S31), F34 (≠ R32), I35 (≠ C33), K43 (≠ P41), D62 (vs. gap), I63 (vs. gap), L81 (= L67), A82 (= A68), A83 (≠ S69), I124 (≠ L111), T126 (≠ S113), K153 (= K143), Y176 (≠ P166), T178 (≠ L168), V179 (= V169), R185 (≠ K175), M188 (≠ L178)
binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (≠ A71), R88 (≠ S74), T126 (≠ S113), S127 (≠ I114), S128 (≠ K115), F149 (≠ Y139), F177 (≠ P167), T178 (≠ L168), R185 (≠ K175), M188 (≠ L178), A189 (vs. gap), R192 (≠ S180), T204 (≠ P192), F206 (= F194), Q211 (≠ R199), R213 (= R200), I250 (≠ T236)

Sites not aligning to the query:

binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: 276

2pk3A Crystal structure of a gdp-4-keto-6-deoxy-d-mannose reductase (see paper)
29% identity, 52% coverage: 1:160/307 of query aligns to 1:161/309 of 2pk3A

query
sites
2pk3A

M

M

K

R

V

A

L

L

I

I

T

T

G

G

G

V

S

A

G
|
G

F
|
F

V
|
V

G

G

R

K

Y

Y

L

L

Q

A

G

N

Y

H

F

L

N

T

S

E

D

Q

E

N

Y

V

D

E

V

V

A

F

I

G

T

T

S

S

R
|
R

C

-

K

-

S

N

N

N

I

E

S

A

D

K

F

L

P

P

V

N

Y

V

T

E

I

M

P

I

A

S

I

L

D
|
D

-
x
I

-

M

Q

D

H

S

T

Q

D

R

W

V

K

K

H

K

A

V

L

I

K

S

N

D

V

I

-

K

-

P

D

D

V

Y

V

I

V

F

H

H

L
|
L

A
|
A

S
x
A

R

K

A
x
S

H

S

S
x
V

S

K

D

D

N

-

H

-

S

S

E

W

A

L

A

N

K

K

E

K

D

G

F

T

T

F

K

S

T

T

N

N

V

V

L

F

G

G

L

T

S

L

K

H

L

V

L

L

D

D

-

A

-

V

-

R

-

D

-

S

-

N

-

L

D

D

C

C

I

-

F

-

L

-

G

-

V

-

K

-

R

R

I

I

I

L

Y

T

L
x
I

S

G

S
|
S

I
x
S

K
x
E

A

E

L

Y

G

G

E

M

S

I

T

L

D

P

G

E

R

E

D

S

A

P

F

V

S

S

E

E

S

E

D

N

K

Q

Y

L

N

R

P

P

A

M

D

S

Y

P

Y
|
Y

G

G

I

V

T

S

K
|
K

A

A

R

S

A

V

E

G

Q

M

L

L

V

A

V

R

E

Q

K

Y

C

V

K

K

H

A

S

Y

G

G

L

M

D

D

active site: S114 (= S113), S115 (≠ I114), E116 (≠ K115), Y140 (= Y139), K144 (= K143)
binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: G10 (= G10), F11 (= F11), V12 (= V12), R32 (= R32), D47 (= D49), I48 (vs. gap), L69 (= L67), A70 (= A68), A71 (≠ S69), S73 (≠ A71), I112 (≠ L111), Y140 (= Y139), K144 (= K143)
binding guanosine-5'-diphosphate-alpha-d-mannose: S73 (≠ A71), V75 (≠ S73), S114 (= S113), S115 (≠ I114), Y140 (= Y139)

Sites not aligning to the query:

Q6T1X6 GDP-6-deoxy-D-mannose reductase; EC 1.1.1.281 from Aneurinibacillus thermoaerophilus (see paper)
29% identity, 52% coverage: 1:160/307 of query aligns to 1:161/309 of Q6T1X6

query
sites
Q6T1X6

M

M

K

R

V

A

L

L

I

I

T

T

G

G

G

V

S

A

G

G

F

F

V

V

G

G

R

K

Y

Y

L

L

Q

A

G

N

Y

H

F

L

N

T

S

E

D

Q

E

N

Y

V

D

E

V

V

A

F

I

G

T

T

S

S

R

R

C

-

K

-

S

N

N

N

I

E

S

A

D

K

F

L

P

P

V

N

Y

V

T

E

I

M

P

I

A

S

I

L

D

D

-

I

-

M

Q

D

H

S

T

Q

D

R

W

V

K

K

H

K

A

V

L

I

K

S

N

D

V

I

-

K

-

P

D

D

V

Y

V

I

V

F

H

H

L

L

A

A

S

A

R

K

A

S

H

S

S

V

S

K

D

D

N

-

H

-

S

S

E

W

A

L

A

N

K

K

E

K

D

G

F

T

T

F

K

S

T

T

N

N

V

V

L

F

G

G

L

T

S

L

K

H

L

V

L

L

D

D

-

A

-

V

-

R

-

D

-

S

-

N

-

L

D

D

C

C

I

-

F

-

L

-

G

-

V

-

K

-

R

R

I

I

I

L

Y

T

L

I

S

G

S

S

I

S

K

E

A

E

L

Y

G

G

E

M

S

I

T

L

D

P

G

E

R

E

D

S

A

P

F

V

S

S

E

E

S

E

D

N

K

Q

Y

L

N

R

P

P

A

M

D

S

Y

P

Y

Y

G

G

I

V

T

S

K

K

A

A

R

S

A

V

E

G

Q

M

L

L

V

A

V

R

E

Q

K

Y

C

V

K

K

H

A

S

Y

G

G

L

M

D

D

Sites not aligning to the query:

169 binding
183 binding
209 binding

Q9SYM5 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1; Protein REPRESSOR OF LRX1 1; Rhamnose biosynthetic enzyme 1; AtRHM1; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
30% identity, 37% coverage: 59:171/307 of query aligns to 79:191/669 of Q9SYM5

query
sites
Q9SYM5

K

E

N

G

V

I

D

D

V

T

V

I

V

M

H

H

L

F

A

A

S

A

R

Q

A

T

H

H

S

V

S

D

D

N

N

S

H

F

S

G

E

N

A

S

A

F

K

-

E

-

D

E

F

F

T

T

K

K

T

N

N

N

V

I

L

Y

G

G

L

T

S

H

K

V

L

L

D

E

D

A

C

C

I

K

F

V

L

T

G

G

-

Q

V

I

K

R

R

R

I

F

I

I

Y

H

L

V

S

S

S

T

I

D

K

E

A

V

L

Y

G

G

E

E

S

T

-

D

T

E

D

D

G

A

R

L

D

V

A

G

F

N

S

H

E

E

S

A

D

S

K

Q

Y

L

N

L

P

P

A

T

D

N

Y

P

Y

Y

G

S

I

A

T

T

K

K

A

A

R

G

A

A

E

E

Q

M

L

L

V

V

V

M

E

A

K

Y

C

G

K

R

H

S

S

Y

G

G

L

L

D

P

Y

V

T

I

I

T

R

R

P

G

P

N

L

N

V

V

Y

Y

G

G

Sites not aligning to the query:

283 R→K: In rol1-2; Abolishes dehydratase activity in vitro (PubMed:16766693). Induces aberrant accumulation of flavonols leading to alterations in plant growth and cell shape formation (PubMed:18567791, PubMed:18757557).

Q9R1J0 Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; EC 1.1.1.170 from Mus musculus (Mouse) (see 3 papers)
29% identity, 49% coverage: 2:152/307 of query aligns to 28:174/362 of Q9R1J0

query
sites
Q9R1J0

K

K

V

C

L

T

I

V

T

I

G

G

S

S

G

G

F

F

V

L

G

G

R

Q

Y

H

L

M

Q

V

G

E

Y

Q

F

L

N

L

S

E

D

R

E

G

Y

Y

D

T

V
|
V

A

N

I

V

T

F

S

D

R

I

C

H

K

Q

S

G

N

F

I

D

S

N

D

P

F

R

P

V

V

Q

Y

F

T

F

I

I

P

G

A

D

I

L

D

C

Q

N

H

Q

T

Q

D

D

W

L

K

Y

H

P

A

A

L

L

K

K

N

G

V

V

D

S

V

T

V

V

V

F

H

H

L

C

A
|
A

S

S

R

P

A

P

H
x
P

S

Y

S

S

D

N

N

N

H

-

S

-

E

-

A

-

K
|
K

E
|
E

D
x
L

F
|
F

T
x
Y

K
x
R

T
x
V

N
|
N

V
x
F

L
x
I

G
|
G

L
x
T

S
x
K

K
x
T

L
x
V

L
x
I

D
x
E

D
x
T

C
|
C

I
x
R

F
x
E

L
x
A

G
|
G

V
|
V

K
x
Q

R
x
K

I
x
L

I
|
I

Y
x
L

L
x
T

S
|
S

I
x
A

K
x
S

A
x
V

L
x
V

G
x
F

E
|
E

S
x
G

T
x
V

D
|
D

G
x
I

R
x
K

D
x
N

A
x
G

F

-

S
x
T

E
|
E

S
x
D

D
x
L

K
x
P

Y
|
Y

-
x
A

-
x
M

N
x
K

P
|
P

A
x
I

D
|
D

Y
|
Y

G
x
T

I
x
E

T
|
T

K
|
K

A
x
I

R
x
L

A
x
Q

E
|
E

Q
x
R

L
x
A

V
|
V

V
x
L

E
x
D

V53 (= V27) to D: in bpa; does not rescue the yeast mutant lacking the ortholog erg26
A94 (= A68) to T: in bpa; does not rescue the yeast mutant lacking the ortholog erg26
P98 (≠ H72) to L: in str; does not rescue the yeast mutant lacking the ortholog erg26
V109 (≠ T88) to M: in str; rescues the yeast mutant lacking the ortholog erg26

Sites not aligning to the query:

103:362 natural variant: Missing (in bpa)
359:362 Prevents secretion from ER

3st7A Crystal structure of capsular polysaccharide assembling protein capf from staphylococcus aureus (see paper)
25% identity, 81% coverage: 1:250/307 of query aligns to 1:219/369 of 3st7A

query
sites
3st7A

M

M

K

N

V

I

L

V

I

I

T

T

G

G

G

A

S

K

G

G

F

F

V

V

G

G

R

K

Y

N

L

L

Q

K

G

A

Y

D

F

L

N

T

S

S

D

-

E

-

Y

-

D

-

V

-

A

-

I

-

T

-

S

-

R

-

C

-

K

-

S

-

N

-

I

T

S

T

D

D

F

H

P

H

V

I

Y

F

T

E

I

V

P

H

A

R

I

Q

D

T

Q

K

H

E

T

E

D

E

W

L

K

E

H

S

A

A

L

L

K

L

N

K

V

A

D

D

V

F

V

I

V

V

H

H

L

L

A

A

S

G

R

V

A

-

H

-

S

-

D

-

N

N

H

R

S

P

E

E

A

H

A

D

K

K

E

E

D

-

F

F

T

S

K

L

T

G

N

N

V

V

L

S

G

Y

L

L

S

D

K

H

L

V

L

L

D

D

D

I

C

L

I

T

F

R

L

N

G

T

V

K

K

K

R

P

I

A

I

I

Y

L

L

L

S

S

-

S

I

I

K

Q

A

A

L

T

G

Q

E

D

S

-

T

-

D

-

G

-

R

-

D

-

A

-

F

-

S

-

E

-

S

-

D

-

K

-

Y

-

N

N

P

P

A

-

D

-

Y

-

Y

Y

G

G

I

E

T

S

K

K

A

L

R

Q

A

G

E

E

Q

Q

L

L

V

L

V

R

E

E

K

Y

C

A

K

E

H

E

S

Y

G

G

L

N

D

T

Y

V

T

Y

I

I

I

Y

R

R

P

W

P

P

L

N

V

L

Y

F

G

G

K

K

S

W

A

C

K

K

A

P

N

N

L

Y

Q

N

S

S

I

V

A

I

R

A

G

T

L

F

S

C

Y

Y

S

K

L

I

P

A

F

R

P

N

Y

E

F

E

T

I

S

Q

S

V

N

N

R

D

R

R

S

N

L

V

I

E

S

L

V

T

H

L

S

N

L

Y

C

V

R

D

F

D

I

I

V

V

-

A

-

E

-

I

-

K

-

R

A

A

V

I

A

E

N

G

D

T

P

P

N

T

T

I

N

E

N

N

Q

G

I

V

Y

P

N

T

V

V

A

P

D

N

N

V

Y

F

V

K

L

V

S

T

T

L

K

G

D

E

I

I

F

V

S

D

L

L

I

L

K

Y

S

K

C

F

S

K

N

Q

S

S

K

R

Sites not aligning to the query:

binding zinc ion: 288, 290, 295, 337

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
25% identity, 79% coverage: 3:243/307 of query aligns to 6:253/310 of 6dntA

query
sites
6dntA

V

V

L

V

I

V

T

T

G
|
G

G

G

S

L

G
|
G

F
|
F

V
x
I

G

G

R

S

Y

H

L

I

Q

V

G

D

Y

A

F

L

N

-

S

I

D

D

E

D

Y

N

D

K

V

V

A

T

I

I

T

I

S
x
D

R
x
N

C
x
L

K
x
S

S
|
S

-
x
G

-

K

-

M

-

E

-

N

-

L

N

N

I

N

S

P

D

N

F

H

P

E

V

N

Y

L

T

T

I

I

P

I

A

K

I

E

D
|
D

-
x
L

Q

M

H

D

T

A

D

D

W

L

K

E

H

K

A

I

L

L

K

K

N

D

V

K

D

D

V

Y

V

V

V

F

H

H

L
|
L

A
|
A

S
x
A

R

L

A
|
A

H

S

S

V

S

P

D

G

N

S

H

V

S

A

E

E

A

P

A

L

K

R

E

-

D

-

F

Y

T

N

K

Q

T

N

N

N

V

I

L

D

G

A

L

S

S

L

K

K

L

L

L

F

D

I

D

A

C

C

I

K

F

N

L

N

G

N

V

I

K

K

R

K

I

V

I

I

Y

F

L
x
S

S
|
S

I
x
S

K
x
A

A

V

L

Y

G

G

E

E

S

N

T

P

D

N

G

M

R

-

D

-

A

P

F

L

S

K

E

E

S

S

D

E

K

N

Y

F

N

L

P

P

A

C

D

S

Y

P

Y
|
Y

G

A

I

A

T

Q

K
|
K

A

A

R

S

A

C

E

E

Q

L

L

Y

V

L

V

K

E

S

K

F

C

H

K

E

H

S

S

Y

G

G

L

L

D

D

Y

Y

T

V

I

A

I

L

R

R

P
x
Y

P
x
F

L
x
N

V
|
V

Y

F

G

G

K

P

S

R

A

Q

K

D

A

E

N

N

-

S

-

P

-

Y

-

A

-
x
A

-
x
V

-

I

-

P

-
x
K

-

F

L

I

Q

S

S

A

I

I

A

L

R

N

G

G

L

E

S

S

Y

-

S

-

L

-

P

P

F
x
V

P
x
I

Y
|
Y

F

G

T

D

S

G

S
x
E

N
x
Q

R

S

R
|
R

S

D

L

F

I

I

S

Y

V

V

H

K

S

E

L

I

C

A

R

K

F

A

I

N

V

I

A

L

V

S

A

A

N

E

D

-

P

-

N

S

T

D

N

Y

N

N

Q

G

I

V

Y

I

N

N

V

V

A

A

D

L

N

G

Y

K

V

S

L

M

S

T

T
x
I

K

N

D

R

I

L

F

F

S

E

L

I

I

I

active site: S120 (= S113), S121 (≠ I114), A122 (≠ K115), Y144 (= Y139), K148 (= K143), A187 (vs. gap)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (= A71), S120 (= S113), S121 (≠ I114), Y144 (= Y139), F172 (≠ P167), N173 (≠ L168), A187 (vs. gap), V188 (vs. gap), K191 (vs. gap), V203 (≠ F191), I204 (≠ P192), Y205 (= Y193), Q210 (≠ N198), R212 (= R200), I246 (≠ T236)
binding nicotinamide-adenine-dinucleotide: G10 (= G7), G13 (= G10), F14 (= F11), I15 (≠ V12), D33 (≠ S31), N34 (≠ R32), L35 (≠ C33), S36 (≠ K34), S37 (= S35), G38 (vs. gap), D57 (= D49), L58 (vs. gap), L76 (= L67), A77 (= A68), A78 (≠ S69), A80 (= A71), S118 (≠ L111), S119 (= S112), Y144 (= Y139), K148 (= K143), Y171 (≠ P166), V174 (= V169)
binding zinc ion: E209 (≠ S197)

Sites not aligning to the query:

3m2pB The crystal structure of udp-n-acetylglucosamine 4-epimerase from bacillus cereus
26% identity, 56% coverage: 1:171/307 of query aligns to 2:158/255 of 3m2pB

query
sites
3m2pB

M

L

K

K

V

I

L

A

I

V

T

T

G
|
G

G

G

S

T

G
|
G

F
|
F

V
x
L

G

G

R

Q

Y

Y

L

V

Q

V

G

E

Y

S

F

I

N

K

S

N

D

D

E

G

Y

N

D

T

V

P

A

I

I

I

T

L

S

T

R
|
R

C

S

-

I

-

G

-

Y

K

E

S

Y

N

R

I

V

S

S

D

D

F

Y

P

T

V

L

Y

-

T

-

I

-

P

-

A

-

I

-

D

-

Q

-

H

-

T

E

D

D

W

L

K

I

H

N

A

Q

L

L

K

N

N

D

V

V

D

D

V

A

V

V

H

H

L

L

A

A

S
x
A

R

T

A

R

H

G

S

S

S

Q

D

G

N

K

H

I

S

S

E

E

A

F

A

H

K

D

E

N

D

E

F

I

T
x
L

K

T

T

Q

N

N

V

-

L

-

G

-

L

-

S

-

K

-

L

L

L

Y

D

D

D

A

C

C

I

Y

F

E

L

N

G

N

V

I

K

S

R

N

I

I

I

V

Y

Y

L
x
A

S
|
S

S
x
T

I

I

K

S

A

A

L

Y

G

S

E

D

S

E

T

T

D

S

G

-

R

-

D

L

A

P

F

W

S

N

E

E

S

K

D

E

K

L

Y

P

N

L

P

P

A

D

D

L

Y
x
M

Y
|
Y

G

G

I

V

T
x
S

K
|
K

A

L

R

A

A

C

E

E

Q

H

L

I

V

G

V

N

E

I

K

Y

C

S

K

R

H

K

S

K

G

G

L

L

D

C

Y

I

T

K

I

N

I

L

R

R

P

F

P

A

L

H

V

L

Y

Y

G

G

active site: A100 (≠ L111), S101 (= S112), T102 (≠ S113), M125 (≠ Y138), Y126 (= Y139), S129 (≠ T142), K130 (= K143)
binding uridine-5'-diphosphate: G8 (= G7), G11 (= G10), F12 (= F11), L13 (≠ V12), R33 (= R32), A64 (≠ S69), L81 (≠ T86)

6vloA X-ray structure of the r141 sugar 4,6-dehydratase from acanthamoeba polyphaga minivirus (see paper)
27% identity, 56% coverage: 3:173/307 of query aligns to 6:185/319 of 6vloA

query
sites
6vloA

V

I

L

L

I

V

T

T

G
|
G

G

G

S

L

G
|
G

F
|
F

V
x
I

G

G

R

S

Y

N

L

F

Q

V

G

N

Y

H

F

I

N

S

S

S

D

K

E

Y

Y

D

D

N

V

V

A

N

I

I

-

Y

-

V

-

Y

-
x
D

-
x
I

-

G

-

D

-

Y

-

C

-
x
A

-

S

-

V

-

E

-

N

-

V

-

E

-

W

-

N

-

R

T

T

S

K

R

L

C

I

K

K

S

G

N
x
D

I
|
I

S

R

D

N

F

F

P

D

V

L

Y

I

T

-

I

-

P

-

A

-

I

-

D

-

Q

M

H

H

T

T

D

L

W

T

K

E

H

H

A

E

L

-

K

-

N

-

V

I

D

D

V

T

V

I

V

V

H

H

L
x
F

A
|
A

S
x
A

R

H

A
x
S

H

H

S

V

S

D

D

N

N

S

H

F

S

K

E

N

A

S

A

L

K

A

E

-

D

-

F

F

T

T

K

E

T

T

N

N

V

V

L

F

G

G

L

T

S

H

K

V

L

L

D

E

D

C

C

S

I

R

F

M

L

Y

G

G

-

K

V

L

K

K

R

L

I

F

Y

H

L
x
M

S

S

S
x
T

I
x
D

K
x
E

A

V

L

Y

G

G

E

E

S

-

T

I

D

D

G

T

R

T

D

D

A

T

F

S

S

R

E

E

S

V

D

S

K

L

Y

L

N

C

P

P

A

T

D

N

Y

P

Y
|
Y

G

A

I

A

T

T

K
|
K

A

A

R

G

A

A

E

E

Q

H

L

I

V

V

V

K

E

S

K

Y

C

F

K

L

H

S

S

Y

G

K

L

L

D

P

Y

I

T

I

I

I

I

A

R

R

P

C

P

N

L
x
N

V

V

Y

Y

G

G

K

R

S

N

active site: T126 (≠ S113), D127 (≠ I114), E128 (≠ K115), Y151 (= Y139), K155 (= K143)
binding nicotinamide-adenine-dinucleotide: G10 (= G7), G13 (= G10), F14 (= F11), I15 (≠ V12), D36 (vs. gap), I37 (vs. gap), A42 (vs. gap), D59 (≠ N36), I60 (= I37), F81 (≠ L67), A82 (= A68), A83 (≠ S69), S85 (≠ A71), M124 (≠ L111), T126 (≠ S113), K155 (= K143)
binding thymidine-5'-diphosphate: N180 (≠ L168)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 190, 191, 194, 206, 208, 215, 250, 275, 281

Query Sequence

>6938128 FitnessBrowser__SB2B:6938128
MKVLITGGSGFVGRYLQGYFNSDEYDVAITSRCKSNISDFPVYTIPAIDQHTDWKHALKN
VDVVVHLASRAHSSDNHSEAAKEDFTKTNVLGLSKLLDDCIFLGVKRIIYLSSIKALGES
TDGRDAFSESDKYNPADYYGITKARAEQLVVEKCKHSGLDYTIIRPPLVYGKSAKANLQS
IARGLSYSLPFPYFTSSNRRSLISVHSLCRFIVAVANDPNTNNQIYNVADNYVLSTKDIF
SLIKSCSNSKSFIFPIPKFIFTFLAFFWSKFGDVSTKLYGSLEINNTKATKVISWKPLEK
PEKNDFV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory