SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 7023349 FitnessBrowser__ANA3:7023349 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
33% identity, 81% coverage: 59:310/310 of query aligns to 65:315/315 of 5vg6B

query
sites
5vg6B

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active site: M98 (vs. gap), R230 (= R225), D254 (= D249), E259 (= E254), H278 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (= A68), R92 (≠ G89), M102 (= M95), L147 (≠ T142), G148 (= G143), D149 (≠ S144), L150 (≠ I145), W168 (≠ I163), S169 (≠ N164), R170 (= R165), T171 (≠ S166), K173 (≠ R168), L201 (= L196), P202 (= P197), T207 (= T202), V228 (= V223), R230 (= R225), H278 (= H273), A280 (≠ S275), S281 (≠ A276), Y315 (= Y310)

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
30% identity, 79% coverage: 61:304/310 of query aligns to 58:305/306 of 5mh6A

query
sites
5mh6A

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binding 2-Ketohexanoic acid: R64 (≠ F67), A65 (= A68), G66 (= G69), H89 (≠ F91), R224 (= R225), H272 (= H273), Y280 (≠ H279)
binding magnesium ion: T130 (≠ S131), A132 (≠ Q133), F210 (≠ L211), E211 (≠ A212), M213 (≠ L214), G225 (= G226), P226 (≠ D227), V228 (≠ L229), E230 (≠ L231), D241 (≠ P242), S251 (≠ T252)
binding nicotinamide-adenine-dinucleotide: A65 (= A68), G66 (= G69), T86 (≠ K88), H89 (≠ F91), G142 (= G143), T143 (≠ S144), L144 (≠ I145), R164 (= R165), P196 (= P197), T201 (= T202), V222 (= V223), A223 (≠ G224), R224 (= R225), H272 (= H273), S274 (= S275)

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 79% coverage: 61:304/310 of query aligns to 60:307/308 of 5mhaB

query
sites
5mhaB

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binding 2-Ketohexanoic acid: R66 (≠ F67), R226 (= R225), H274 (= H273), Y282 (≠ H279)
binding (2R)-2-hydroxyhexanoic acid: R66 (≠ F67), A67 (= A68), G68 (= G69), H91 (≠ F91), Y282 (≠ H279)
binding magnesium ion: F212 (≠ L211), E213 (≠ A212), M215 (≠ L214), D243 (≠ P242)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A68), G68 (= G69), T88 (≠ K88), L143 (≠ T142), G144 (= G143), T145 (≠ S144), L146 (≠ I145), R165 (≠ N164), R166 (= R165), S167 (= S166), P180 (≠ L179), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 79% coverage: 61:304/310 of query aligns to 60:307/308 of 5mhaA

query
sites
5mhaA

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binding 2-Ketohexanoic acid: R66 (≠ F67), A67 (= A68), G68 (= G69), H91 (≠ F91), R226 (= R225), H274 (= H273), Y282 (≠ H279)
binding magnesium ion: T132 (≠ S131), A134 (≠ Q133)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A68), G68 (= G69), T88 (≠ K88), L143 (≠ T142), G144 (= G143), T145 (≠ S144), L146 (≠ I145), R165 (≠ N164), R166 (= R165), S167 (= S166), P180 (≠ L179), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
30% identity, 79% coverage: 61:304/310 of query aligns to 60:307/308 of 5mh5A

query
sites
5mh5A

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D

Q

A

Q

Q

R

H

Q

D

K

H

S

A

W

W

Q

D

V

L

-

P

Q

R

S

Y

A

E

T

E

R

P

G

F

S
x
T

L

L

Q
x
A

G

G

M

E

R

R

L

V

L

C

L

V

G
|
G

T
x
L

G
|
G

S
x
T

I
x
L

A

G

Q

R

H

G

V

V

A

V

K

D

T

R

A

A

K

A

H

A

F

L

G

G

M

M

H

E

I

V

T

V

G

G

I

V

N
x
R

R
|
R

S
|
S

G

G

R

D

E

P

V

V

D

D

G

N

F

V

D

S

L

T

I

V

Q

Y

P

T

L

P

S

D

Q

R

L

L

G

H

Q

E

C

A

L

I

S

A

Q

D

S

A

D

R

V

F

V

V

T

V

N

L

L

A

L
x
T

P
|
P

S

L

T

T

P

D

E

E

T
|
T

R

E

L

G

L

M

L

V

N

A

A

A

D

P

M

E

L
x
F

A
x
E

T

T

L
x
M

K

R

A

E

D

D

A

A

I

S

L

L

V

V

N

N

V
|
V

G
x
A

R
|
R

G

G

D

P

V

V

L

V

D

V

L

E

D

S

A

D

L

L

N

V

T

A

Q

A

L

L

I

D

A

S

R

G

P
x
D

E

I

Q

A

H

G

A

A

V

A

L

L

D

D

V

V

F

F

T

S

Q

E

E

E

P

P

L

L

P

P

A

E

S

D

H

S

P

P

I

L

W

W

E

D

R

F

P

E

N

D

A

V

I

L

I

I

T

T

P

P

H
|
H

I

V

S
|
S

A

A

P

A

S

T

-

S

-

K

H
x
Y

P

H

E

E

Q

D

I

V

V

A

R

A

I

L

F

I

S

R

D

E

N

N

Y

I

R

E

R

K

Y

I

I

A

A

T

A

G

E

D

P

E

L

L

Q

T

N

N

Q

R

V

V

binding 2-Ketohexanoic acid: R66 (≠ F67), A67 (= A68), G68 (= G69), H91 (≠ F91), R226 (= R225), H274 (= H273), Y282 (≠ H279)
binding magnesium ion: T132 (≠ S131), A134 (≠ Q133), F212 (≠ L211), E213 (≠ A212), M215 (≠ L214), D243 (≠ P242)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A68), G68 (= G69), T88 (≠ K88), G142 (= G141), L143 (≠ T142), G144 (= G143), T145 (≠ S144), L146 (≠ I145), R165 (≠ N164), R166 (= R165), S167 (= S166), T197 (≠ L196), P198 (= P197), T203 (= T202), V224 (= V223), A225 (≠ G224), R226 (= R225), H274 (= H273), S276 (= S275)

4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
33% identity, 94% coverage: 21:310/310 of query aligns to 26:316/316 of 4zqbB

query
sites
4zqbB

A

A

Q

R

Q

A

L

L

P

P

G

G

L

-

E

E

L

R

L

I

D

D

D

G

-

F

-

R

-

E

-

L

N

S

P

P

A

A

N

E

I

R

A

A

E

E

A

V

N

D

I

I

W

A

L

I

A

V

E

A

P

N

K

P

L

D

A

P

A

A

P

D

L

L

V

A

P

-

H

E

A

L

K

P

Q

N

L

L

K

V

W

W

L

I

Q

H

S

S

S

L

F

W

A

A

G

G

I

V

D

E

A

R

L

L

-

V

-

A

-

E

M

L

G

G

P

H

R

L

A

A

R

R

K

P

D

I

Y

V

Q
x
R

L

L

T

V

N

D

I

P

K

E

G

-

I

-

F
x
L

G

A

P

R

L

T

M
|
M

S

A

E

E

Y

A

L

A

F

L

G

A

Y

W

L

T

L

Y

A

Y

H

L

V

F

R

R

G

D

H

M

Q

P

F

A

Y

Y

Q

A

A

A

Q

Q

R

R

Q

A

K

R

S

V

W

W

Q

K

V

G

Q

L

S

P

A

Y

T

K

R

R

G

P

S

-

L

-

Q

E

G

R

M

T

R

T

L

V

L

G

L

V

L

L

G
|
G

T
x
L

G
|
G

S
x
E

I
x
L

A

G

Q

A

H

A

V

A

A

A

K

L

T

R

A

L

K

R

H

D

F

A

G

G

M

F

H

D

I

V

T

H

G

G

I
x
W

N
x
S

R
|
R

S
|
S

G

P

R
x
K

E

E

V

I

D

A

G

G

F

V

D

T

L

C

I

H

Q

A

P

G

L

E

S

E

Q

T

L

L

G

E

Q

R

C

M

L

L

S

G

Q

Q

S

V

D

E

V

I

V

L

T

V

N

C

L

L

L
|
L

P
|
P

S

L

T

T

P

G

E

E

T

T

R

R

L

G

L

L

N

D

A

A

D

R

M

R

L

L

A

A

T

C

L

L

K

P

A

E

D

G

A

A

I

Q

L

I

V

V

N

N

V
x
F

G

A

R
|
R

G

G

D

P

V

I

L

L

D

D

L

S

D

A

A

A

L

L

N

I

T

E

Q

A

L

L

I

D

A

S

R

G

P

R

E

I

Q

G

H

H

A

A

V

V

L

L

D

D

V

V

F

F

T

E

Q

V

E
|
E

P

P

L

L

P

P

A

E

S

A

H

S

P

P

I

F

W

W

E

G

R

H

P

P

N

K

A

V

I

T

I

V

T

L

P

P

H
|
H

I

I

S
|
S

A
|
A

P

A

S

T

H

D

P

P

E

E

Q

T

I

A

V

S

R

A

I

I

F

V

S

G

D

A

N

H

Y

V

R

A

R

D

Y

Y

I

R

A

A

A

T

E

G

P

R

L

I

Q

P

N

P

Q

S

V

V

D

D

F

L

I

T

Q

R

G

G

Y
|
Y

active site: L99 (≠ F91), R231 (= R225), E260 (= E254), H279 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (≠ Q83), M103 (= M95), G147 (= G141), L148 (≠ T142), G149 (= G143), E150 (≠ S144), L151 (≠ I145), W169 (≠ I163), S170 (≠ N164), R171 (= R165), S172 (= S166), K174 (≠ R168), L202 (= L196), P203 (= P197), F229 (≠ V223), R231 (= R225), H279 (= H273), S281 (= S275), A282 (= A276), Y316 (= Y310)

4z0pA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
28% identity, 94% coverage: 21:310/310 of query aligns to 20:316/316 of 4z0pA

query
sites
4z0pA

A

A

Q

G

Q

A

L

F

P

P

G

G

L

R

E

E

L

V

L

I

D

D

-

L

D

A

N

D

P

P

A

A

N

H

-

Q

-

E

-

R

-

D

-

L

-

S

-

G

I

I

A

D

E

Y

A

A

N

V

I

V

W
|
W

L

K

A

S

E

A

P

P

K

D

L

L

A

F

A

S

P

-

L

-

V

-

P

-

H

R

A

A

K

P

Q

D

L

L

K

K

W

V

L

V

Q

F

S

S

S

G

F
x
G

A
|
A

G
|
G

I

V

D

D

A

H

L

V

M

L

G

T

P

L

R

P

A

G

R

L

K

P

D

D

Y

V

Q

P

L

L

T

V

N
x
R

-

F

I

V

K

D

G

R

I

T

F
x
L

G

T

P

T

L

R

M
|
M

S

S

E

E

Y

W

L

V

F

M

G

M

Y

Q

L

C

L

L

A

L

H

H

V

L

R

R

G

Q

H
|
H

Q
x
R

F

A

Y

Y

Q

E

A

A

Q

L

Q

A

R

K

Q

K

K

H

S

E

W

W

Q

R

V

D

Q

L

S

S

A

Q

T

P

R

E

G

A

S

A

L

-

Q

-

G

D

M

V

R

T

L

V

L

G

L

I

L

M

G

G

T
x
M

G
|
G

S
x
V

I
x
L

A

G

Q

Q

H

D

V

A

A

A

K

R

T

K

A

L

K

A

H

A

F

M

G

G

M

F

H

K

I

V

T

I

G

G

I
x
W

N
x
S

R
|
R

S
|
S

G

K

R
|
R

E

V

V

I

D

E

G

G

F

V

D

E

L

T

I

Y

Q

D

P

A

L

-

S

A

Q

G

L

L

G

D

Q

A

C

F

L

L

S

G

Q

R

S

T

D

D

V

F

V

L

T

V

N

G

L

L

L
|
L

P
|
P

S

L

T

T

P

P

E

D

T

T

R

R

L

G

L

I

L

F

N

N

A

A

D

A

M

L

L

F

A

A

T

K

L

L

K

S

A

R

D

N

A

G

-

P

-

F

-

G

-

A

-

P

I

V

L

F

V

I

N

N

V
x
A

G
|
G

R
|
R

G

G

-

G

-

S

-

Q

-

V

D

E

V

A

L

D

D

I

L

L

D

E

A

C

L

L

N

D

T

S

Q

G

L

V

I

L

A
x
G

R

-

P

-

E

-

Q

-

H

G

A

A

V

S

L

L

D
|
D

V

V

F

F

T

E

Q

R

E
|
E

P

P

L

L

P

S

A

P

S

E

H

S

P

R

I

F

W

W

E

D

R

M

P

P

N

N

A

V

I

Y

I

V

T

T

P

P

H
|
H

I

V

S
x
A

A
|
A

P

S

S

S

H

D

P

V

E

R

Q

A

I

L

V

F

R

V

I

H

F

V

S

E

D

H

N

Q

Y

I

R

A

R

R

Y

F

I

E

A

S

A

G

E

L

P

P

L

L

Q

E

N

H

Q

V

V

V

D

D

F

K

I

V

Q

A

G

G

Y
|
Y

active site: L95 (≠ F91), R231 (= R225), G250 (≠ A240), D255 (= D249), E260 (= E254), H279 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (≠ N86), M99 (= M95), M144 (≠ T142), G145 (= G143), V146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), S168 (= S166), R170 (= R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), H279 (= H273), A281 (≠ S275), A282 (= A276), Y316 (= Y310)
binding oxalic acid: W50 (= W43), G70 (≠ F67), A71 (= A68), G72 (= G69), H114 (= H110), R115 (≠ Q111), R231 (= R225), H279 (= H273)

4weqA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADP and sulfate (see paper)
28% identity, 94% coverage: 21:310/310 of query aligns to 20:316/316 of 4weqA

query
sites
4weqA

A

A

Q

G

Q

A

L

F

P

P

G

G

L

R

E

E

L

V

L

I

D

D

-

L

D

A

N

D

P

P

A

A

N

H

-

Q

-

E

-

R

-

D

-

L

-

S

-

G

I

I

A

D

E

Y

A

A

N

V

I

V

W

W

L

K

A

S

E

A

P

P

K

D

L

L

A

F

A

S

P

-

L

-

V

-

P

-

H

R

A

A

K

P

Q

D

L

L

K

K

W

V

L

V

Q

F

S

S

S

G

F

G

A

A

G

G

I

V

D

D

A

H

L

V

M

L

G

T

P

L

R

P

A

G

R

L

K

P

D

D

Y

V

Q

P

L

L

T

V

N
x
R

-

F

I

V

K

D

G

R

I

T

F
x
L

G

T

P

T

L

R

M
|
M

S

S

E

E

Y

W

L

V

F

M

G

M

Y

Q

L

C

L

L

A

L

H

H

V

L

R

R

G

Q

H

H

Q

R

F

A

Y

Y

Q

E

A

A

Q

L

Q

A

R

K

Q

K

K

H

S

E

W

W

Q

R

V

D

Q

L

S

S

A

Q

T

P

R

E

G

A

S

A

L

-

Q

-

G

D

M

V

R

T

L

V

L

G

L

I

L

M

G

G

T
x
M

G
|
G

S
x
V

I
x
L

A

G

Q

Q

H

D

V

A

A

A

K

R

T

K

A

L

K

A

H

A

F

M

G

G

M

F

H

K

I

V

T

I

G

G

I
x
W

N
x
S

R
|
R

S
|
S

G

K

R
|
R

E

V

V

I

D

E

G

G

F

V

D

E

L

T

I

Y

Q

D

P

A

L

-

S

A

Q

G

L

L

G

D

Q

A

C

F

L

L

S

G

Q

R

S

T

D

D

V

F

V

L

T

V

N

G

L

L

L
|
L

P
|
P

S

L

T

T

P

P

E

D

T

T

R

R

L

G

L

I

L

F

N

N

A

A

D

A

M

L

L

F

A

A

T

K

L

L

K

S

A

R

D

N

A

G

-

P

-

F

-

G

-

A

-

P

I

V

L

F

V

I

N

N

V
x
A

G
|
G

R
|
R

G

G

-

G

-

S

-

Q

-

V

D

E

V

A

L

D

D

I

L

L

D

E

A

C

L

L

N

D

T

S

Q

G

L

V

I

L

A
x
G

R

-

P

-

E

-

Q

-

H

G

A

A

V

S

L

L

D
|
D

V

V

F

F

T

E

Q

R

E
|
E

P

P

L

L

P

S

A

P

S

E

H

S

P

R

I

F

W

W

E

D

R

M

P

P

N

N

A

V

I

Y

I

V

T

T

P

P

H
|
H

I

V

S
x
A

A

A

P

S

S

S

H

D

P

V

E

R

Q

A

I

L

V

F

R

V

I

H

F

V

S

E

D

H

N

Q

Y

I

R

A

R

R

Y

F

I

E

A

S

A

G

E

L

P

P

L

L

Q

E

N

H

Q

V

V

V

D

D

F

K

I

V

Q

A

G

G

Y
|
Y

active site: L95 (≠ F91), R231 (= R225), G250 (≠ A240), D255 (= D249), E260 (= E254), H279 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R89 (≠ N86), M99 (= M95), M144 (≠ T142), G145 (= G143), V146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), S168 (= S166), R170 (= R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), D255 (= D249), H279 (= H273), A281 (≠ S275), Y316 (= Y310)

5bqfA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADP, hepes and l(+)-tartaric acid
27% identity, 84% coverage: 51:310/310 of query aligns to 55:317/317 of 5bqfA

query
sites
5bqfA

A

A

P

D

L

L

V

F

P

R

H

R

A

A

K

P

Q

N

L

L

K

K

W

V

L

I

Q

F

S

S

S

G

F

G

A
|
A

G

G

I

V

D

D

A

H

L

I

M

I

G

G

P

M

R

A

A

G

R

L

K

P

D

D

Y

I

Q

P

L

I

T

V

N
x
R

-

F

I

V

K

D

G

R

I

S

F

L

G

T

P

T

L

R

M
|
M

S

S

E

E

Y

W

L

V

F

V

G

M

Y

Q

L

C

L

L

A

M

H

H

V

L

R

R

G

G

H

Q

Q

Y

F

G

Y

H

Q

D

A

S

Q

H

Q

Q

R

R

Q

R

K

R

S

E

W

W

Q

A

V

K

Q

L

S

I

A

A

T

P

R

E

G

A

S

A

L

-

Q

-

G

E

M

V

R

T

L

V

L

G

L

V

L

M

G
|
G

T
x
L

G
|
G

S
x
I

I
x
L

A

G

Q

Q

H

D

V

A

A

V

K

A

T

K

A

L

K

K

H

V

F

M

G

G

M

F

H

N

I

V

T

I

G

G

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R

K

E

T

V

I

D

E

G

G

F

V

D

E

L

T

I

F

Q

D

P

A

L

-

S

G

Q

E

L

L

G

D

Q

R

C

F

L

L

S

A

Q

K

S

T

D

D

V

I

V

L

T

V

N

G

L

L

L
|
L

P
|
P

S

L

T

T

P

P

E

E

T

T

R

T

L

G

L

F

L

Y

N

D

A

S

D

E

M

L

L

F

A

K

T

K

L

L

K

R

A

R

D

D

A

G

-

A

-

L

-

G

-

Q

-

P

I

V

L

F

V

I

N

N

V
x
A

G
|
G

R
|
R

G

G

D

K

V

-

L

-

D

-

L

-

D

S

A

Q

L

I

N

E

T

T

Q

D

L

I

I

V

A

S

R

A

P

V

E

R

Q

E

H

G

-

T

-

L

-

G

A

A

V

S

L

L

D

D

V

V

F

F

T

E

Q

V

E
|
E

P

P

L

L

P

A

A

T

S

D

H

S

P

P

I

L

W

W

E

E

R

L

P

E

N

N

A

V

I

F

I

I

T

T

P

P

H
|
H

I

D

S

A

A
|
A

P

V

S

S

H

E

P

E

E

N

Q

A

I

L

V

F

R

R

I

H

F

V

S

E

D

M

N

Q

Y

I

R

A

R

R

Y

F

I

E

A

R

A

G

E

E

P

P

L

L

Q

Q

N

F

Q

V

V

I

D

D

F

R

I

A

Q

A

G

G

Y
|
Y

active site: E261 (= E254), H280 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A72 (= A68), R90 (≠ N86), M100 (= M95), G144 (= G141), L145 (≠ T142), G146 (= G143), I147 (≠ S144), L148 (≠ I145), W166 (≠ I163), S167 (≠ N164), R168 (= R165), T169 (≠ S166), L198 (= L196), P199 (= P197), A230 (≠ V223), G231 (= G224), R232 (= R225), H280 (= H273), A283 (= A276), Y317 (= Y310)

4xcvA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH
27% identity, 84% coverage: 51:310/310 of query aligns to 55:317/317 of 4xcvA

query
sites
4xcvA

A

A

P

D

L

L

V

F

P

R

H

R

A

A

K

P

Q

N

L

L

K

K

W

V

L

I

Q

F

S

S

S

G

F

G

A
|
A

G

G

I

V

D

D

A

H

L

I

M

I

G

G

P

M

R

A

A

G

R

L

K

P

D

D

Y

I

Q

P

L

I

T

V

N
x
R

-

F

I

V

K

D

G

R

I

S

F

L

G

T

P

T

L

R

M
|
M

S

S

E

E

Y

W

L

V

F

V

G

M

Y

Q

L

C

L

L

A

M

H

H

V

L

R

R

G

G

H

Q

Q

Y

F

G

Y

H

Q

D

A

S

Q

H

Q

Q

R

R

Q

R

K

R

S

E

W

W

Q

A

V

K

Q

L

S

I

A

A

T

P

R

E

G

A

S

A

L

-

Q

-

G

E

M

V

R

T

L

V

L

G

L

V

L

M

G
|
G

T
x
L

G
|
G

S
x
I

I
x
L

A

G

Q

Q

H

D

V

A

A

V

K

A

T

K

A

L

K

K

H

V

F

M

G

G

M

F

H

N

I

V

T

I

G

G

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R
x
K

E

T

V

I

D

E

G

G

F

V

D

E

L

T

I

F

Q

D

P

A

L

-

S

G

Q

E

L

L

G

D

Q

R

C

F

L

L

S

A

Q

K

S

T

D

D

V

I

V

L

T

V

N

G

L

L

L
|
L

P
|
P

S

L

T

T

P

P

E

E

T

T

R

T

L

G

L

F

L

Y

N

D

A

S

D

E

M

L

L

F

A

K

T

K

L

L

K

R

A

R

D

D

A

G

-

A

-

L

-

G

-

Q

-

P

I

V

L

F

V

I

N

N

V
x
A

G
|
G

R
|
R

G

G

D

K

V

-

L

-

D

-

L

-

D

S

A

Q

L

I

N

E

T

T

Q

D

L

I

I

V

A

S

R

A

P

V

E

R

Q

E

H

G

-

T

-

L

-

G

A

A

V

S

L

L

D

D

V

V

F

F

T

E

Q

V

E
|
E

P

P

L

L

P

A

A

T

S

D

H

S

P

P

I

L

W

W

E

E

R

L

P

E

N

N

A

V

I

F

I

I

T

T

P

P

H
|
H

I

D

S

A

A
|
A

P

V

S

S

H

E

P

E

E

N

Q

A

I

L

V

F

R

R

I

H

F

V

S

E

D

M

N

Q

Y

I

R

A

R

R

Y

F

I

E

A

R

A

G

E

E

P

P

L

L

Q

Q

N

F

Q

V

V

I

D

D

F

R

I

A

Q

A

G

G

Y
|
Y

active site: E261 (= E254), H280 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A72 (= A68), R90 (≠ N86), M100 (= M95), G144 (= G141), L145 (≠ T142), G146 (= G143), I147 (≠ S144), L148 (≠ I145), W166 (≠ I163), S167 (≠ N164), R168 (= R165), T169 (≠ S166), K171 (≠ R168), L198 (= L196), P199 (= P197), A230 (≠ V223), G231 (= G224), R232 (= R225), H280 (= H273), A283 (= A276), Y317 (= Y310)

5tsdA Crystal structure of NADPH-dependent 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH and oxalate
27% identity, 84% coverage: 51:310/310 of query aligns to 54:316/316 of 5tsdA

query
sites
5tsdA

A

A

P

D

L

L

V

F

P

R

H

R

A

A

K

P

Q

N

L

L

K

K

W

V

L

I

Q

F

S

S

S

G

F
x
G

A
|
A

G
|
G

I

V

D

D

A

H

L

I

M

I

G

G

P

M

R

A

A

G

R

L

K

P

D

D

Y

I

Q

P

L

I

T

V

N
x
R

-

F

I

V

K

D

G

R

I

S

F

L

G

T

P

T

L

R

M
|
M

S

S

E

E

Y

W

L

V

F

V

G

M

Y

Q

L

C

L

L

A

M

H

H

V

L

R

R

G

G

H

Q

Q

Y

F

G

Y

H

Q

D

A

S

Q

H

Q

Q

R

R

Q

R

K

R

S

E

W

W

Q

A

V

K

Q

L

S

I

A

A

T

P

R

E

G

A

S

A

L

-

Q

-

G

E

M

V

R

T

L

V

L

G

L

V

L

M

G
|
G

T
x
L

G
|
G

S
x
I

I
x
L

A

G

Q

Q

H

D

V

A

A

V

K

A

T

K

A

L

K

K

H

V

F

M

G

G

M

F

H

N

I

V

T

I

G

G

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R
x
K

E

T

V

I

D

E

G

G

F

V

D

E

L

T

I

F

Q

D

P

A

L

-

S

G

Q

E

L

L

G

D

Q

R

C

F

L

L

S

A

Q

K

S

T

D

D

V

I

V

L

T

V

N

G

L

L

L
|
L

P
|
P

S

L

T

T

P

P

E

E

T

T

R

T

L

G

L

F

L

Y

N

D

A

S

D

E

M

L

L

F

A

K

T

K

L

L

K

R

A

R

D

D

A

G

-

A

-

L

-

G

-

Q

-

P

I

V

L

F

V

I

N

N

V
x
A

G
|
G

R
|
R

G

G

D

K

V

-

L

-

D

-

L

-

D

S

A

Q

L

I

N

E

T

T

Q

D

L

I

I

V

A

S

R

A

P

V

E

R

Q

E

H

G

-

T

-

L

-

G

A

A

V

S

L

L

D
|
D

V

V

F

F

T

E

Q

V

E
|
E

P

P

L

L

P

A

A

T

S

D

H

S

P

P

I

L

W

W

E

E

R

L

P

E

N

N

A

V

I

F

I

I

T

T

P

P

H
|
H

I

D

S

A

A

A

P

V

S

S

H

E

P

E

E

N

Q

A

I

L

V

F

R

R

I

H

F

V

S

E

D

M

N

Q

Y

I

R

A

R

R

Y

F

I

E

A

R

A

G

E

E

P

P

L

L

Q

Q

N

F

Q

V

V

I

D

D

F

R

I

A

Q

A

G

G

Y
|
Y

active site: E260 (= E254), H279 (= H273)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A71 (= A68), R89 (≠ N86), M99 (= M95), G143 (= G141), L144 (≠ T142), G145 (= G143), I146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), T168 (≠ S166), K170 (≠ R168), L197 (= L196), P198 (= P197), A229 (≠ V223), G230 (= G224), R231 (= R225), D255 (= D249), H279 (= H273), Y316 (= Y310)
binding oxalic acid: G70 (≠ F67), A71 (= A68), G72 (= G69), R231 (= R225), H279 (= H273)

Sites not aligning to the query:

binding oxalic acid: 50

7jqiA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with alpha-ketoglutarate and NADP+
31% identity, 71% coverage: 91:310/310 of query aligns to 95:312/312 of 7jqiA

query
sites
7jqiA

F

M

G

G

P

E

L

Q

M
|
M

S

Q

E

E

Y

Y

L

A

F

V

G

S

Y

Q

L

V

L

L

A

H

H

W

V

F

R

R

G

R

H

F

Q

D

F

D

Y

Y

Q

R

A

I

Q

Q

R

N

Q

S

K

S

S

H

W

W

Q

Q

V

-

Q

-

S

P

A

L

T

P

R

E

G

Y

S

H

L

R

Q

E

G

D

M

F

R

T

L

I

L

G

L

I

L

L

G

G

T
x
A

G
|
G

S
x
V

I
x
L

A

G

Q

S

H

K

V

V

A

A

K

Q

T

S

A

L

K

Q

H

T

F

W

G

R

M

F

H

P

I

L

T

R

G

C

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R
x
K

E

S

V

W

D

P

G

G

F

V

D

Q

L

S

I

F

Q

A

P

G

L

R

S

E

Q

E

L

L

G

S

Q

A

C

F

L

L

S

S

Q

Q

S

C

D

R

V

V

V

L

T

I

N

N

L

L

L
|
L

P
|
P

S

N

T

T

P

P

E

E

T

T

R

V

L

G

L

I

N

N

A

Q

D

Q

M

L

L

L

A

E

T

K

L

L

K

P

A

D

D

G

A

A

I

Y

L

L

V

L

N

N

V
x
L

G

A

R
|
R

G

G

D

V

V

H

L

V

D

V

L

E

D

D

A

D

L

L

N

L

T

A

Q

A

L

L

I

D

A

S

R

G

P

K

E

V

Q

K

H

G

A

A

V

M

L

L

D

D

V

V

F

F

T

N

Q

R

E

E

P

P

L

L

P

P

A

P

S

E

H

S

P

P

I

L

W

W

E

Q

R

H

P

P

N

R

A

V

I

T

I

I

T

T

P

P

H
|
H

I

V

S

A

A
|
A

P

I

S

T

H

R

P

P

E

A

Q

E

I

A

V

V

R

E

I

Y

F

I

S

S

D

R

N

T

Y

I

R

A

R

Q

Y

L

I

E

A

K

A

G

E

E

P

K

L

V

Q

C

N

G

Q

Q

V

V

D

D

F

R

I

A

Q

R

G

G

Y
|
Y

binding 2-oxoglutaric acid: R227 (= R225), H275 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (= M95), A144 (≠ T142), G145 (= G143), V146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), T168 (≠ S166), K170 (≠ R168), L198 (= L196), P199 (= P197), L225 (≠ V223), R227 (= R225), H275 (= H273), A278 (= A276), Y312 (= Y310)

Sites not aligning to the query:

6p35A 2.5 angstrom structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with 2-keto arginine and NADP
31% identity, 71% coverage: 91:310/310 of query aligns to 95:312/312 of 6p35A

query
sites
6p35A

F

M

G

G

P

E

L

Q

M
|
M

S

Q

E

E

Y

Y

L

A

F

V

G

S

Y

Q

L

V

L

L

A

H

H

W

V

F

R

R

G

R

H

F

Q

D

F

D

Y

Y

Q

R

A

I

Q

Q

R

N

Q

S

K

S

S

H

W

W

Q

Q

V

-

Q

-

S

P

A

L

T

P

R

E

G

Y

S

H

L

R

Q

E

G

D

M

F

R

T

L

I

L

G

L

I

L

L

G

G

T
x
A

G
|
G

S
x
V

I
x
L

A

G

Q

S

H

K

V

V

A

A

K

Q

T

S

A

L

K

Q

H

T

F

W

G

R

M

F

H

P

I

L

T

R

G

C

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R
x
K

E

S

V

W

D

P

G

G

F

V

D

Q

L

S

I

F

Q

A

P

G

L

R

S

E

Q

E

L

L

G

S

Q

A

C

F

L

L

S

S

Q

Q

S

C

D

R

V

V

V

L

T

I

N

N

L

L

L
|
L

P
|
P

S

N

T

T

P

P

E

E

T

T

R

V

L

G

L

I

N

N

A

Q

D

Q

M

L

L

L

A

E

T

K

L

L

K

P

A

D

D

G

A

A

I

Y

L

L

V

L

N

N

V
x
L

G

A

R
|
R

G

G

D

V

V

H

L

V

D

V

L

E

D

D

A

D

L

L

N

L

T

A

Q

A

L

L

I

D

A

S

R

G

P

K

E

V

Q

K

H

G

A

A

V

M

L

L

D

D

V

V

F

F

T

N

Q

R

E

E

P

P

L

L

P

P

A

P

S

E

H

S

P

P

I

L

W

W

E

Q

R

H

P

P

N

R

A

V

I

T

I

I

T

T

P

P

H
|
H

I

V

S
x
A

A
|
A

P

I

S
x
T

H

R

P

P

E

A

Q

E

I

A

V

V

R

E

I

Y

F

I

S

S

D

R

N

T

Y

I

R

A

R

Q

Y

L

I

E

A

K

A

G

E

E

P

K

L

V

Q

C

N

G

Q

Q

V

V

D

D

F

R

I

A

Q

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (= M95), A144 (≠ T142), G145 (= G143), V146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), T168 (≠ S166), K170 (≠ R168), L198 (= L196), P199 (= P197), L225 (≠ V223), R227 (= R225), H275 (= H273), A277 (≠ S275), A278 (= A276), Y312 (= Y310)
binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: R227 (= R225), H275 (= H273), T280 (≠ S278)

Sites not aligning to the query:

7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
31% identity, 71% coverage: 91:310/310 of query aligns to 96:313/313 of 7jqhA

query
sites
7jqhA

F

M

G

G

P

E

L

Q

M
|
M

S

Q

E

E

Y

Y

L

A

F

V

G

S

Y

Q

L

V

L

L

A

H

H

W

V

F

R

R

G

R

H

F

Q

D

F

D

Y

Y

Q

R

A

I

Q

Q

R

N

Q

S

K

S

S

H

W

W

Q

Q

V

-

Q

-

S

P

A

L

T

P

R

E

G

Y

S

H

L

R

Q

E

G

D

M

F

R

T

L

I

L

G

L

I

L

L

G

G

T
x
A

G
|
G

S
x
V

I
x
L

A

G

Q

S

H

K

V

V

A

A

K

Q

T

S

A

L

K

Q

H

T

F

W

G

R

M

F

H

P

I

L

T

R

G

C

I
x
W

N
x
S

R
|
R

S
x
T

G

R

R
x
K

E

S

V

W

D

P

G

G

F

V

D

Q

L

S

I

F

Q

A

P

G

L

R

S

E

Q

E

L

L

G

S

Q

A

C

F

L

L

S

S

Q

Q

S

C

D

R

V

V

V

L

T

I

N

N

L

L

L
|
L

P
|
P

S

N

T

T

P

P

E

E

T

T

R

V

L

G

L

I

N

N

A

Q

D

Q

M

L

L

L

A

E

T

K

L

L

K

P

A

D

D

G

A

A

I

Y

L

L

V

L

N

N

V
x
L

G
x
A

R
|
R

G

G

D

V

V

H

L

V

D

V

L

E

D

D

A

D

L

L

N

L

T

A

Q

A

L

L

I

D

A

S

R

G

P

K

E

V

Q

K

H

G

A

A

V

M

L

L

D
|
D

V

V

F

F

T

N

Q

R

E

E

P

P

L

L

P

P

A

P

S

E

H

S

P

P

I

L

W

W

E

Q

R

H

P

P

N

R

A

V

I

T

I

I

T

T

P

P

H
|
H

I

V

S

A

A
|
A

P

I

S

T

H

R

P

P

E

A

Q

E

I

A

V

V

R

E

I

Y

F

I

S

S

D

R

N

T

Y

I

R

A

R

Q

Y

L

I

E

A

K

A

G

E

E

P

K

L

V

Q

C

N

G

Q

Q

V

V

D

D

F

R

I

A

Q

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: M100 (= M95), A145 (≠ T142), G146 (= G143), V147 (≠ S144), L148 (≠ I145), W166 (≠ I163), S167 (≠ N164), R168 (= R165), T169 (≠ S166), K171 (≠ R168), L199 (= L196), P200 (= P197), L226 (≠ V223), A227 (≠ G224), R228 (= R225), D252 (= D249), H276 (= H273), A279 (= A276), Y313 (= Y310)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 66, 90

3kboA 2.14 angstrom crystal structure of putative oxidoreductase (ycdw) from salmonella typhimurium in complex with NADP
28% identity, 90% coverage: 31:310/310 of query aligns to 35:312/312 of 3kboA

query
sites
3kboA

D

D

D
x
N

N

N

P

P

A

A

N

D

I

Y

A

A

E

L

A

-

N

-

I

V

W

W

L

Q

A

P

E

P

P

V

K

E

L

M

A

L

A
|
A

P

-

L

-

V

-

P

-

H

-

A

G

K
x
R

Q
x
R

L

L

K

K

W

A

L

V

Q

F

S

V

S

L

F

G

A

A

G

G

I

V

D

D

A

A

L

I

M

L

G

S

P

K

R

L

A

N

R

A

K

H

D

P

Y

E

Q

M

L

L

T

D

N

A

I

S

K

I

G

P

I

L

F

F

-
x
R

-

L

-

E

-

D

-

T

-

G

-
x
M

G

G

P

L

L

Q

M
|
M

S

Q

E

E

Y

Y

L

A

F

V

G

S

Y

Q

L

V

L

L

A

H

H

W

V

F

R

R

G

R

H

F

Q

D

F

D

Y

Y

Q

Q

A

A

Q

L

Q

K

R

N

Q

Q

K

A

S

L

W

W

Q

K

-

P

V

L

Q

P

S

E

A

Y

T

T

R

R

G

-

S

-

L

-

Q

E

G

E

M

F

R

S

L

V

L

G

L

I

L

M

G
|
G

T
x
A

G
|
G

S
x
V

I
x
L

A

G

Q

A

H

K

V

V

A

A

K

E

T

S

A

L

K

Q

H

A

F

W

G

G

M

F

H

P

I

L

T

R

G

C

I
x
W

N
x
S

R
|
R

S
|
S

G

R

R
x
K

E

S

V

W

D

P

G

G

F

V

D

E

L

S

I

Y

Q

V

P

G

L

R

S

E

Q

E

L

L

G

R

Q

A

C

F

L

L

S

N

Q

Q

S

T

D

R

V

V

V

L

T

I

N

N

L
|
L

L

L

P
|
P

S

N

T

T

P

A

E

Q

T

T

R

V

L

G

L

I

N

N

A

S

D

E

M

L

L

L

A

D

T

Q

L

L

K

P

A

D

D

G

A

A

I

Y

L

V

V

L

N

N

V
x
L

G
x
A

R
|
R

G

G

D

V

V

H

L

V

D

Q

L

E

D

A

A

D

L

L

N

L

T

A

Q

A

L

L

I

D

A

S

R

G

P

K

E

L

Q

K

H

G

A

A

V

M

L

L

D
|
D

V

V

F

F

T

S

Q

Q

E
|
E

P

P

L

L

P

P

A

Q

S

E

H

S

P

P

I

L

W

W

E

R

R

H

P

P

N

R

A

V

I

A

I

M

T

T

P

P

H
|
H

I

I

S

A

A
|
A

P

V

S

T

H

R

P

P

E

A

Q

E

I

A

V

I

R

D

I

Y

F

I

S

S

D

R

N

T

Y

I

R

T

R

Q

Y

L

I

E

A

K

A

G

E

E

P

P

L

V

Q

T

N

G

Q

Q

V

V

D

D

F

R

I

A

Q

R

G

G

Y
|
Y

active site: M95 (vs. gap), R227 (= R225), E256 (= E254), H275 (= H273)
binding N-(2-hydroxyethyl)-N,N-dimethyl-3-sulfopropan-1-aminium: N36 (≠ D32), A53 (= A51), R55 (≠ K58), R56 (≠ Q59)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (vs. gap), M99 (= M95), G143 (= G141), A144 (≠ T142), G145 (= G143), V146 (≠ S144), L147 (≠ I145), W165 (≠ I163), S166 (≠ N164), R167 (= R165), S168 (= S166), K170 (≠ R168), L197 (= L195), P199 (= P197), L225 (≠ V223), A226 (≠ G224), R227 (= R225), D251 (= D249), H275 (= H273), A278 (= A276), Y312 (= Y310)

1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
31% identity, 81% coverage: 27:278/310 of query aligns to 36:283/304 of 1wwkA

query
sites
1wwkA

L

V

E

E

L

L

L

V

D

K

D

D

N

-

P

-

A

-

N

-

I

-

A

V

E

E

A

A

N

I

I

I

W

V

L

R

A

S

E

K

P

P

K

K

L

V

A

T

A

R

P

R

L

V

V

I

P

E

H

S

A

A

K

P

Q

K

L

L

K

K

W

V

L

I

Q

A

S

R

S

A

F

G

A

V

G

G

I

L

D

D

A

N

L

I

M

D

G

V

P

E

R

A

A

A

R

K

-

E

K

K

D

G

Y

I

Q

E

L

V

T

V

N

N

I

A

K

P

G

A

I

A

F
x
S

G

S

P

R

L

S

M
x
V

S

A

E

E

Y

L

L

A

F

V

G

G

Y

L

L

M

L

F

A

S

H

V

V

A

R

R

G

K

H

I

Q

A

F

F

Y

A

Q

D

A

R

Q

K

Q

M

R

R

Q

E

K

G

S

V

W

W

Q

A

V

K

Q

K

S

E

A

A

T

M

R

G

G

I

S

E

L

L

Q

E

G

G

M

K

R

T

L

I

L

G

L

I

G
|
G

T
x
F

G
|
G

S
x
R

I
|
I

A

G

Q

Y

H

Q

V

V

A

A

K

K

T

I

A

A

K

N

H

A

F

L

G

G

M

M

H

N

I

I

T

L

G

L

I
x
Y

N
x
D

-
x
P

-

Y

-

P

-

N

-

E

R

E

S

R

G

A

R

K

E

E

V

V

D

N

G

G

F

-

D

-

L

-

I

-

Q

-

P

K

L

F

S

V

Q

D

L

L

G

E

Q

T

C

L

L

L

S

K

Q

E

S

S

D

D

V

V

T

T

N

I

L

H

L
x
V

P
|
P

S

L

T

V

P

E

E

S

T
|
T

R

Y

L

H

L

L

L

I

N

N

A

E

D

E

M

R

L

L

A

K

T

L

L

M

K

K

A

K

D

T

A

A

I

I

L

L

V

I

N

N

V
x
T

G
x
S

R
|
R

G

G

D

P

V

V

L

V

D

D

L

T

D

N

A

A

L

L

N

V

T

K

Q

A

L

L

I

K

A

E

R

G

P

W

E

I

Q

A

H

G

A

A

V

G

L

L

D
|
D

V

V

F

F

T

E

Q

E

E
|
E

P

P

L

L

P

P

A

K

S

D

H

H

P

P

I

L

W

T

E

K

R

F

P

D

N

N

A

V

I

V

I

L

T

T

P

P

H
|
H

I

I

S
x
G

A

A

P

S

S

T

active site: S96 (≠ F91), R230 (= R225), D254 (= D249), E259 (= E254), H278 (= H273)
binding nicotinamide-adenine-dinucleotide: V100 (≠ M95), G146 (= G141), F147 (≠ T142), G148 (= G143), R149 (≠ S144), I150 (= I145), Y168 (≠ I163), D169 (≠ N164), P170 (vs. gap), V201 (≠ L196), P202 (= P197), T207 (= T202), T228 (≠ V223), S229 (≠ G224), D254 (= D249), H278 (= H273), G280 (≠ S275)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
29% identity, 72% coverage: 56:278/310 of query aligns to 64:293/334 of 5aovA

query
sites
5aovA

H

N

A

A

K

P

Q

R

L

L

K

R

W

I

L

V

Q

A

S

N

S
x
Y

F
x
A

A
x
V

G
|
G

I

Y

D

D

A

N

L

I

M

D

G

V

P

E

R

E

A

A

-

T

R

R

K

R

D

G

Y

I

Q

Y

L

V

T

T

N

N

I

T

K

P

G

D

I

V

F
x
L

G

T

P

N

L

A

M
x
T

S

A

E

D

Y

H

L

A

F

F

G

A

Y

L

L

L

A

A

H

T

-

A

-

R

-

H

-

V

V

V

R

K

G

G

H

D

Q

K

F

F

Y

V

Q

R

A

S

Q

G

Q

E

R

W

Q

K

K

R

-

K

-

G

-

I

S

A

W

W

Q

H

V

P

Q

K

S

W

A

F

T

L

R

G

G

Y

S

E

L

L

Q

Y

G

G

M

K

R

T

L

I

L

G

L

I

L

V

G

G

T
x
F

G
|
G

S
x
R

I
|
I

A

G

Q

Q

H

A

V

I

A

A

K

R

T

R

A

A

K

K

H

G

F

F

G

N

M

M

H

R

I

I

T

L

G

Y

I

Y

N
x
S

R
|
R

S

T

G

R

R

K

E

S

V

Q

D

A

G

E

F

K

D

E

L

L

I

G

Q

A

P

E

L

Y

S

R

Q

P

L

L

G

E

Q

E

C

V

L

L

S

K

Q

E

S

S

D

D

V

F

V

V

T

I

N

L

L
x
A

L
x
V

P
|
P

S

L

T

T

P

K

E

E

T
|
T

R

M

L

Y

L

M

L

I

N

N

A

E

D

E

M

R

L

L

A

K

T

L

L

M

K

K

A

P

D

T

A

A

I

I

L

L

V

V

N

N

V
x
I

G
x
A

R
|
R

G

G

D

K

V

V

L

V

D

D

L

T

D

K

A

A

L

L

N

I

T

K

Q

A

L

L

I

K

A

E

R

G

P

W

E

I

Q

A

H

G

A

A

V

G

L

L

D
|
D

V

V

F

F

T

E

Q

E

E
|
E

P

P

L

Y

P

-

A

Y

S

N

H

E

P

E

I

L

W

F

E

S

R

L

P

D

N

N

A

V

I

V

I

L

T

T

P

P

H
|
H

I

I

S
x
G

A

S

P

A

S

T

active site: L100 (≠ F91), R241 (= R225), D265 (= D249), E270 (= E254), H288 (= H273)
binding glyoxylic acid: Y74 (≠ S66), A75 (≠ F67), V76 (≠ A68), G77 (= G69), R241 (= R225), H288 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A68), T104 (≠ M95), F158 (≠ T142), G159 (= G143), R160 (≠ S144), I161 (= I145), S180 (≠ N164), R181 (= R165), A211 (≠ L195), V212 (≠ L196), P213 (= P197), T218 (= T202), I239 (≠ V223), A240 (≠ G224), R241 (= R225), H288 (= H273), G290 (≠ S275)

Sites not aligning to the query:

binding glyoxylic acid: 52, 53, 53

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
33% identity, 55% coverage: 107:278/310 of query aligns to 119:292/332 of 6biiA

query
sites
6biiA

V

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active site: R240 (= R225), D264 (= D249), E269 (= E254), H287 (= H273)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G156 (= G141), F157 (≠ T142), G158 (= G143), R159 (≠ S144), I160 (= I145), A179 (≠ N164), R180 (= R165), S181 (= S166), K183 (≠ R168), V211 (≠ L196), P212 (= P197), E216 (= E201), T217 (= T202), V238 (= V223), A239 (≠ G224), R240 (= R225), D264 (= D249), H287 (= H273), G289 (≠ S275)

Sites not aligning to the query:

active site: 99
binding nadp nicotinamide-adenine-dinucleotide phosphate: 75, 103

1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
28% identity, 61% coverage: 95:282/310 of query aligns to 106:295/404 of 1psdA

query
sites
1psdA

M

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active site: R234 (= R225), D258 (= D249), E263 (= E254), H286 (= H273)
binding nicotinamide-adenine-dinucleotide: H155 (≠ S144), I156 (= I145), D175 (≠ N164), I176 (≠ R165), K179 (≠ R168), H204 (≠ L195), V205 (≠ L196), P206 (= P197), A232 (≠ V223), S233 (≠ G224), R234 (= R225), H286 (= H273)

Sites not aligning to the query:

active site: 102
binding nicotinamide-adenine-dinucleotide: 102
binding serine: 338, 340, 341, 344

P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 61% coverage: 95:282/310 of query aligns to 112:301/410 of P0A9T0

query
sites
P0A9T0

M

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HI 161:162 (≠ SI 144:145) binding
D181 (≠ N164) binding
ASR 238:240 (≠ VGR 223:225) binding
D264 (= D249) binding
HIGG 292:295 (≠ HISA 273:276) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>7023349 FitnessBrowser__ANA3:7023349
MGHKLLLLTKANEQYRQLIEAQQLPGLELLDDNPANIAEANIWLAEPKLAAPLVPHAKQL
KWLQSSFAGIDALMGPRARKDYQLTNIKGIFGPLMSEYLFGYLLAHVRGHQFYQAQQRQK
SWQVQSATRGSLQGMRLLLLGTGSIAQHVAKTAKHFGMHITGINRSGREVDGFDLIQPLS
QLGQCLSQSDVVTNLLPSTPETRLLLNADMLATLKADAILVNVGRGDVLDLDALNTQLIA
RPEQHAVLDVFTQEPLPASHPIWERPNAIITPHISAPSHPEQIVRIFSDNYRRYIAAEPL
QNQVDFIQGY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory