SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 8501746 FitnessBrowser__Miya:8501746 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

6uqnB Crystal structure of r173a variant of cytosolic fumarate hydratase from leishmania major in a complex with fumarate and s-malate (see paper)
40% identity, 93% coverage: 13:174/175 of query aligns to 364:527/532 of 6uqnB

query
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6uqnB

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binding fumaric acid: R385 (= R34), T431 (= T80), T432 (= T81), R435 (= R84)

Sites not aligning to the query:

binding fumaric acid: 107, 108, 189
binding iron/sulfur cluster: 106, 108, 189, 224, 225, 319, 321

6msnA Crystal structure of cytosolic fumarate hydratase from leishmania major in a complex with inhibitor thiomalate (see paper)
40% identity, 93% coverage: 13:174/175 of query aligns to 364:527/532 of 6msnA

query
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6msnA

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binding (2S)-2-sulfanylbutanedioic acid: R385 (= R34), T431 (= T80), T432 (= T81), R435 (= R84)
binding iron/sulfur cluster: K455 (= K103)

Sites not aligning to the query:

binding (2S)-2-sulfanylbutanedioic acid: 107, 108, 146, 189
binding iron/sulfur cluster: 106, 108, 224, 225, 319, 321

6uoiA Crystal structure of cytosolic fumarate hydratase from leishmania major in a complex with malonate (see paper)
40% identity, 93% coverage: 13:174/175 of query aligns to 367:530/535 of 6uoiA

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6uoiA

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binding fe3-s4 cluster: K458 (= K103)
binding fe (iii) ion: T435 (= T81), R438 (= R84)

Sites not aligning to the query:

binding fe3-s4 cluster: 108, 226, 227, 321, 323
binding fe (iii) ion: 194

6unzA Crystal structure of cytosolic fumarate hydratase from leishmania major (see paper)
40% identity, 93% coverage: 13:174/175 of query aligns to 371:534/539 of 6unzA

query
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6unzA

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binding iron/sulfur cluster: K462 (= K103)

Sites not aligning to the query:

binding iron/sulfur cluster: 113, 114, 115, 231, 232, 326, 328

E9AE57 Fumarate hydratase 2; Fumarase 2; LmFH-2; EC 4.2.1.2 from Leishmania major (see paper)
40% identity, 93% coverage: 13:174/175 of query aligns to 400:563/568 of E9AE57

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E9AE57

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R421 (= R34) binding
TTAGR 467:471 (≠ TTSYR 80:84) binding
K491 (= K103) binding

Sites not aligning to the query:

133 binding
134:135 binding
173 binding
216 binding
219:225 binding
252 binding
346 binding

P14407 Fumarate hydratase class I, anaerobic; D-tartrate dehydratase; Fumarase B; EC 4.2.1.2; EC 4.2.1.81 from Escherichia coli (strain K12) (see paper)
40% identity, 97% coverage: 6:174/175 of query aligns to 363:535/548 of P14407

query
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P14407

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Sites not aligning to the query:

192 modified: N6-acetyllysine

6msoA Crystal structure of mitochondrial fumarate hydratase from leishmania major in a complex with inhibitor thiomalate (see paper)
36% identity, 85% coverage: 26:174/175 of query aligns to 387:537/540 of 6msoA

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6msoA

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binding (2S)-2-sulfanylbutanedioic acid: R395 (= R34), T442 (= T81), K465 (= K103)
binding iron/sulfur cluster: K465 (= K103)

Sites not aligning to the query:

binding (2S)-2-sulfanylbutanedioic acid: 106, 107, 145, 188
binding iron/sulfur cluster: 105, 223, 224, 319, 321

Q4QAU9 Fumarate hydratase 1, mitochondrial; Fumarase 1; LmFH-1; EC 4.2.1.2 from Leishmania major (see paper)
36% identity, 85% coverage: 26:174/175 of query aligns to 396:546/549 of Q4QAU9

query
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Q4QAU9

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Sites not aligning to the query:

114 binding
233 binding
328 binding

Query Sequence

>8501746 FitnessBrowser__Miya:8501746
MATHHLTTPLTDEAVAQLRSGDVVFLSGTIYTARDAAHRRLAESLDRGEDPPFDLRGAVI
YYVGPSPAPPGRPIGSAGPTTSYRMDSYAPRLHALGLKATIGKGRRNTEVRDALKQHTAV
YLGATGGAGALLSQCITAATVIAYDDLGPEAIRELTVKDFPLLVINDCVGGELYV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory