SitesBLAST

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
25% identity, 96% coverage: 1:416/434 of query aligns to 26:400/824 of Q8GAI3

query
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Q8GAI3

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W66 (= W55) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (≠ A56) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 68% coverage: 120:416/434 of query aligns to 78:370/377 of Q5L2C2

query
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Q5L2C2

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V180 (vs. gap) binding
R309 (= R356) binding
334:340 (vs. 380:386, 29% identical) binding
R336 (≠ T382) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 68% coverage: 120:416/434 of query aligns to 77:368/368 of 4yshB

query
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4yshB

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active site: I262 (≠ V311), L283 (≠ D338), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
binding flavin-adenine dinucleotide: V178 (vs. gap), S206 (≠ L253), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385), L338 (≠ T386)
binding glycine: G249 (≠ Y298), Y251 (≠ V300), Y251 (≠ V300), A264 (≠ G313), R307 (= R356), R334 (≠ T382), R334 (≠ T382)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48

4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 68% coverage: 120:416/434 of query aligns to 77:368/370 of 4yshA

query
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4yshA

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G

K

C

V

Y

A

I

I

V

T

P

R

K

F

S

D

G

T

N

R

R

I

L

R

L

V
x
I

G

G

G

A

M

T

A

S

E

T

I

P

A

G

G

T

F

F

D

D

L

-

S

-

L

-

N

-

P

-

R

-

R

R

E

V

T

S

L

A

E

G

M

G

I

V

V

M

N

N

D
x
L

L

L

Y

H

P

R

Q

A

G

A

G

H

-

L

-

V

-

P

D

D

L

I

A

E

E

Q

A

A

S

E

F

W

-

V

-

A

-

S

W

W

T

S

G
|
G

L

I

R
|
R

P

P

T

Q

T

T

P

E

D

D

G

G

T

L

P

P

I

Y

V

L

G

G

A

E

T

H

P

P

-

E

F

R

K

R

N

G

L

L

F

F

L

V

N

A

T

A

G

G

H
|
H

G

Y

T
x
R

L
x
N

G
|
G

W
x
I

T
x
L

M

L

A

S

C

P

G

L

S

T

G

G

R

L

L

L

L

V

A

A

D

D

L

L

M

V

A

E

K

R

K

K

T

E

P

T

Q

A

I

F

S

D

A

L

E

A

G

P

L

F

D

S

I

L

S

T

R

R

Y

H

active site: I262 (≠ V311), L283 (≠ D338), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
binding flavin-adenine dinucleotide: V178 (vs. gap), S206 (≠ L253), G207 (= G254), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
24% identity, 92% coverage: 3:402/434 of query aligns to 4:347/363 of 7cyxA

query
sites
7cyxA

V

V

L

A

V

I

L
x
I

G
|
G

S

G

G
|
G

V
|
V

I
|
I

G

G

T

S

A

S

S

V

A

A

Y

H

Y

F

L

L

A

A

R

E

A

R

G

G

F

H

E

K

V

V

T

A

V

I

V
|
V

D
x
E

R
x
K

Q

Q

P

S

A

I

A

A

A

S

M

-

E
|
E

T
x
A

S
|
S

F

K

A

A

N
x
A

A

A

G
|
G

Q
x
L

V

L

S

G

P

V

G

A

Y

Y

A

N

S

-

P

-

W

-

A

-

P

-

G

-

V

-

P

P

L

L

K

-

A

-

I

-

K

-

W

-

L

-

Q

-

R

-

H

-

A

F

P

E

L

L

A

A

I

R

K

E

A

S

T

R

A

A

D

I

I

F

D

P

Q

Q

Y

-

L

-

W

-

M

L

A

A

Q

A

M

V

L

L

R

R

N

E

C

K

T

T

A

G

S

-

R

-

Y

-

A

-

V

-

N

-

K

-

E

-

R

-

M

-

V

-

R

-

L

-

S

-

E

-

Y

-

S

-

R

-

D

-

C

-

L

-

D

-

E

-

L

-

R

-

A

-

E

-

T

V

G

D

I

I

A

G

Y

Y

E

E

G

E

R

K

T

G

L

I

G

-

T

-

Q

-

L

-

F

Y

R

R

T

I

Q

A

A

Q

Q

N

L

E

D

D

G

E

A

K

A

E

K

R

D

I

I

L

A

H

V

I

L

M

-

D

-

W

-

Q

K

Q

E

K

S

T

G

G

V

E

P

D

F

S

E

Y

L

F

L

L

D

T

R

G

A

D

G

H

I

V

A

R

R

E

V

K

E

E

P

P

A

Y

L

L

A

-

S

-

V

-

T

S

D

E

I

S

L

I

A

I

G

G

A

A

L

V

R

Y

L

Y

P

P

N

K

D

D

Q

G

T

H

G

V

D

I

C

A

Q

P

I

E

F

L

T

T

S

K

R

A

L

F

A

A

E

H

M

S

A

A

V

A

K

I

L

S

G

G

V

A

E

D

F

I

R

Y

F

E

D

Q

Q

T

D

E

I
x
V

Q

F

R

D

L

I

D

R

Y

I

A

E

G

N

D

N

R

K

I

V

N

T

G

G

V

V

W

I

I

T

D

S

G

E

K

G

L

I

E

V

T

T

A

C

D

E

R

K

Y

V

V

V

L

I

A

A

L
x
G

G
|
G

S

S

Y
x
W

S

S

P

T

K

K

L

L

K

S

P

Y

L

F

G

H

I

R

K

D

A

W

P

G

V

T

Y

Y

P

P

L

V

K

K

G

G

Y

E

S

-

L

-

T

V

V

V

P

A

I

V

T

R

N

S

P

R

A

K

M

Q

A

L

P

L

T

K

S

A

T

P

I

I

L

F

D

Q

E

E

T

R

Y

F

K

Y

V

I

A

T

I

P

T

K

R

R

F

-

D

G

T

G

R

R

I

Y

R

V

V

I

G

G

G

A

M

T

A

M

E

K

I

P

A

H

G

T

F

F

D

N

L

K

S

T

L

V

N

Q

P

P

R

-

E

E

T

S

L

I

E

T

M

S

I

I

V

L

N

E

D

R

L

A

Y

Y

P

T

Q

I

G

L

G

P

D

A

L

L

A

K

E

E

A

A

-

E

-

W

-

E

S

S

F

T

W

W

T

A

G
|
G

L

L

R
|
R

P
|
P

T

Q

T

S

P

N

D

H

G

E

T

A

P

P

I

Y

V

M

G

G

A

E

-

H

T

E

P

E

F

I

K

K

N

G

L

L

F

Y

L

A

N

C

T

T

G

G

H

H

G
x
Y

T
x
R

L
x
N

G
|
G

W
x
I

T

L

M

L

A

S

C

P

G

I

S

S

G

G

R

Q

L

Y

L

M

A

A

D

D

L

L

M

I

A

E

K

G

K

K

binding flavin-adenine dinucleotide: I7 (≠ L6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (= I11), V30 (= V29), E31 (≠ D30), K32 (≠ R31), E38 (= E38), A39 (≠ T39), S40 (= S40), A43 (≠ N43), G45 (= G45), L46 (≠ Q46), V171 (≠ I224), G200 (≠ L253), G201 (= G254), W203 (≠ Y256), G298 (= G354), R300 (= R356), P301 (= P357), Y326 (≠ G381), R327 (≠ T382), N328 (≠ L383), G329 (= G384), I330 (≠ W385)

2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
23% identity, 80% coverage: 88:433/434 of query aligns to 54:403/403 of 2gagB

query
sites
2gagB

W

W

M

L

A

A

-

G

-
x
G

Q
x
N

M
|
M

L
x
A

R
|
R

N
|
N

C
x
T

T
|
T

A
x
I

S

I

R
|
R

Y

S

A

N

V

Y

N

L

K

W

E

D

R

E

M

S

V

A

R

G

L

I

S

Y

E

E

Y

K

S

S

R

L

D

K

C

L

L

W

D

E

E

Q

L

L

R

P

A

E

E

D

T

L

G

E

I

Y

A

D

Y

F

E

L

G

F

R

S

T

Q

L

R

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

V

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

L

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

D

R

P

A

S

G

Q

I

V

A

K

R

E

V

A

E

C

P

P

A

I

L

I

A

N

S

T

V

S

T

D

D

D

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

W

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D
x
K

C
x
H

Q

D

I

H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q

C

D

E

I
x
V

Q

T

R

G

L

F

D

I

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

H

A

A

D

G

R

K

Y

V

V

A

L

L

A

A

L
x
G

G
x
A

S

G

Y
x
H

S

S

P

S

K

V

L
|
L

L

A

K

E

P

M

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L
|
L

K

Q

G

-

Y

-

S

A

L

L

T
x
V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I
x
M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T

H

R

V

F
x
Y

D

V

T

S

R

Q

I

A

R

H

V
x
K

G

G

G
x
E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

Q

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S
x
R

F

T

W
|
W

T

G

G

G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

R

F

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

N

K

D

T

E

P

P

Q

H

I

E

S

L

A

N

E

K

G

P

L

F

D

S

I

L

S

E

R

R

Y

F

G

-

N

E

T

T

P

G

K

H

E

L

S

I

E

D

K

E

H

H

V

G

N

A

P

A

A

A

P
x
V

A

A

H

H

active site: A61 (≠ L93), T64 (≠ C96), T65 (= T97)
binding flavin-adenine dinucleotide: G58 (vs. gap), N59 (≠ Q91), M60 (= M92), R62 (= R94), N63 (= N95), T64 (≠ C96), I66 (≠ A98), V195 (≠ I224), G224 (≠ L253), A225 (≠ G254), H227 (≠ Y256), L231 (= L260), L246 (= L273), G352 (= G381), T353 (= T382), G354 (≠ L383), G355 (= G384), F356 (≠ W385), K357 (≠ T386)
binding flavin mononucleotide: A61 (≠ L93), R62 (= R94), H171 (≠ C200), V250 (≠ T279), E278 (≠ G313), R321 (≠ S350), W323 (= W352)
binding 2-furoic acid: T64 (≠ C96), I66 (≠ A98), R68 (= R100), M263 (≠ I293), Y270 (≠ F305), K357 (≠ T386), V401 (≠ P431)
binding sulfite ion: K170 (≠ D199), K276 (≠ V311)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52

P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
23% identity, 80% coverage: 88:433/434 of query aligns to 56:405/405 of P40875

query
sites
P40875

W

W

M

L

A

A

-

G

Q

N

M

M

L

A

R

R

N

N

C

T

T

T

A

I

S

I

R

R

Y

S

A

N

V

Y

N

L

K

W

E

D

R

E

M

S

V

A

R

G

L

I

S

Y

E

E

Y

K

S

S

R

L

D

K

C

L

L

W

D

E

E

Q

L

L

R

P

A

E

E

E

T

L

G

D

I

Y

A

D

Y

F

E

L

G

F

R

S

T

Q

L

R

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

V

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

F

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

T

R

P

A

E

G

Q

I

V

A

K

R

E

V

V

E
x
C

P

P

A

I

L

I

A

N

S

I

V

G

T

D

D

D

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

Y

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D

K

C
x
H

Q

D

I
x
H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q
x
C

D

E

I

V

Q

T

R

G

L

F

D

L

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

H

A

A

D

G

R

K

Y

V

V

A

L

L

A

A

L

G

G

A

S

G

Y

H

S

S

P

S

K

V

L

L

L

A

K

E

P

L

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L

L

K

Q

G

-

Y

-

S

A

L

L

T

V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I

M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T

H

R

V

F

Y

D

V

T

S

R

Q

I

A

R

H

V

K

G

G

G

E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

E

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S

R

F

T

W

W

T

G

G

G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T
x
C

G

G

H

W

G

G

T

T

L

G

G

G

W

F

T

K

M

G

A

T

C

P

G

G

S

A

G

G

R

F

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

N

K

D

T

E

P

A

Q

H

I

A

S

L

A

N

E

A

G

P

L

F

D

S

I

L

S

E

R

R

Y

F

G

-

N

E

T

T

P

G

K

H

E

L

S

I

E

D

K

E

H

H

V

G

N

A

P

A

A

A

P

V

A

A

H

H

C146 (≠ E176) mutation to S: No change in activity.
H173 (≠ C200) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
H175 (≠ I202) mutation to A: No effect on FMN binding and activity.
C195 (≠ Q222) mutation to S: No change in activity.
C351 (≠ T378) mutation to A: No change in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.

6j39A Crystal structure of cmis2 with inhibitor (see paper)
26% identity, 74% coverage: 98:416/434 of query aligns to 48:368/368 of 6j39A

query
sites
6j39A

A

A

S
x
N

R

E

Y

M

A

L

V

Y

N

S

K

Q

E

E

R

D

M

L

V

L

R

R

L

L

S

C

E

L

Y

A

S

S

R

R

D

E

-

R

-

Y

-

P

-

S

C

F

L

V

D

K

E

E

L

L

R

E

A

A

E

V

T

S

G

G

I

T

A

S

Y

A

E

G

G

Y

R

R

T

R

L

D

G

G

T

V

T

L

Q

D

L

A

F

A

-

F

-

D

R

E

T

S

Q

L

A

A

Q

A

L

L

D

D

G

G

A

L

A

R

K

N

D

F

I

L

A

A

V

P

L

L

K

-

E

-

S

-

G

G

V

V

P

A

F

V

E

A

L

P

L

L

D

N

R

A

A

R

G

R

I

C

A

R

R

E

V

H

E

E

P

P

A

M

L

L

A

A

S

-

V

-

T

-

D

E

I

S

L

V

A

R

G

G

A

G

L

L

R

L

L

G

P

P

N

D

D

D

Q

G

T

A

G

V

D

N

C

P

Q

R

I

E

F

L

T

T

S

A

R

-

L

-

A

-

E

-

M

-

A

A

V

L

K

L

L

A

G

A

V

I

E

D

F

V

R

R

F

G

D

G

Q

T

D

L

I

I

Q

R

R

R

L

R

D
x
A

-

T

-

E

-

F

Y

L

A

A

G

D

D

E

R

R

I

T

N

P

G

G

V

V

W

L

I

L

D

E

-

N

G

G

K

C

L

A

E

V

T

H

A

G

D

D

R

R

Y

V

V

V

L

L

A

S

L
x
A

G

G

S

C

Y
x
W

S

T

P

H

K

R

L

L

L

A

K

G

-

L

P

P

L

A

G

G

I

A

K

V

A

P

P

E

V

I

Y

A

P

P

L

A

K

K

G

G

Y

Q

S
x
I

L

L

T

R

V

L

P

R

I

S

T

A

N

A

P

P

A

F

M

L

A

R

P

R

T

A

S

T

T
x
R

I

A

L

V

D

T

E

R

T

G

Y

S

K

G

V

V

-
x
Y

A

L

I

V

T

P

R

R

F

T

D

D

T

G

R

E

I

L

R

V

V

V

G

G

G

A

M

T

A
x
Y

E

E

I

E

A

R

G

D

F

Y

D

D

L

T

S

T

L

V

N

T

P

A

R

G

R

V

E

A

T

E

L

L

E

L

M

G

I

K

V

V

N

L

D

A

L

V

Y

L

P

P

Q

G

G

A

D

E

L

L

A

E

E

L

A

A

S

E

F

T

W

A

T

A

G

G

L

L

R
|
R

P

P

T

G

T

S

P

P

D

D

G

G

T

L

P

P

I

V

V

L

G

G

A

W

T

T

P

A

F

V

K

P

N

N

L

L

F

L

L

V

N

A

T

T

G

G

H

H

G
x
S

T
x
R

L
x
I

G
|
G

W
x
V

T
x
Q

M

L

A

A

C

P

G

I

S

T

G

A

R

D

L

V

L

M

A

G

D

E

L

M

M

L

A

V

K

T

-

G

K

R

T

T

P

P

Q

E

I

V

S

-

A

A

E

K

G

A

L

F

D

A

I

V

S

D

R

R

Y

F

binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: N49 (≠ S99), R243 (≠ T292), Y252 (vs. gap), Y267 (≠ A315), R308 (= R356), R334 (≠ T382), I335 (≠ L383)
binding flavin-adenine dinucleotide: A174 (≠ D228), A203 (≠ L253), W206 (≠ Y256), I228 (≠ S277), Y252 (vs. gap), R308 (= R356), S333 (≠ G381), R334 (≠ T382), I335 (≠ L383), G336 (= G384), V337 (≠ W385), Q338 (≠ T386)

Sites not aligning to the query:

binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46
binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44

6j38A Crystal structure of cmis2 (see paper)
26% identity, 74% coverage: 98:416/434 of query aligns to 48:368/368 of 6j38A

query
sites
6j38A

A

A

S

N

R

E

Y

M

A

L

V

Y

N

S

K

Q

E

E

R

D

M

L

V

L

R

R

L

L

S

C

E

L

Y

A

S

S

R

R

D

E

-

R

-

Y

-

P

-

S

C

F

L

V

D

K

E

E

L

L

R

E

A

A

E

V

T

S

G

G

I

T

A

S

Y

A

E

G

G

Y

R

R

T

R

L

D

G

G

T

V

T

L

Q

D

L

A

F

A

-

F

-

D

R

E

T

S

Q

L

A

A

Q

A

L

L

D

D

G

G

A

L

A

R

K

N

D

F

I

L

A

A

V

P

L

L

K

-

E

-

S

-

G

G

V

V

P

A

F

V

E

A

L

P

L

L

D

N

R

A

A

R

G

R

I

C

A

R

R

E

V

H

E

E

P

P

A

M

L

L

A

A

S

-

V

-

T

-

D

E

I

S

L

V

A

R

G

G

A

G

L

L

R

L

L

G

P

P

N

D

D

D

Q

G

T

A

G

V

D

N

C

P

Q

R

I

E

F

L

T

T

S

A

R

-

L

-

A

-

E

-

M

-

A

A

V

L

K

L

L

A

G

A

V

I

E

D

F

V

R

R

F

G

D

G

Q

T

D

L

I

I

Q

R

R

R

L

R

D
x
A

-

T

-

E

-

F

Y

L

A

A

G

D

D

E

R

R

I

T

N

P

G

G

V

V

W

L

I

L

D

E

-

N

G

G

K

C

L

A

E

V

T

H

A

G

D

D

R

R

Y

V

V

V

L

L

A

S

L
x
A

G

G

S

C

Y
x
W

S

T

P

H

K

R

L

L

L

A

K

G

-

L

P

P

L

A

G

G

I

A

K

V

A

P

P

E

V

I

Y

A

P

P

L

A

K

K

G
|
G

Y

Q

S

I

L

L

T

R

V

L

P

R

I

S

T

A

N

A

P

P

A

F

M

L

A

R

P

R

T

A

S

T

T

R

I

A

L

V

D

T

E

R

T

G

Y

S

K

G

V

V

-

Y

A

L

I

V

T

P

R

R

F

T

D

D

T

G

R

E

I

L

R

V

V

V

G

G

G

A

M

T

A

Y

E

E

I

E

A

R

G

D

F

Y

D

D

L

T

S

T

L

V

N

T

P

A

R

G

R

V

E

A

T

E

L

L

E

L

M

G

I

K

V

V

N

L

D

A

L

V

Y

L

P

P

Q

G

G

A

D

E

L

L

A

E

E

L

A

A

S

E

F

T

W

A

T

A

G
|
G

L

L

R
|
R

P

P

T

G

T

S

P

P

D

D

G

G

T

L

P

P

I

V

V

L

G

G

A

W

T

T

P

A

F

V

K

P

N

N

L

L

F

L

L

V

N

A

T

T

G

G

H

H

G
x
S

T
x
R

L
x
I

G
|
G

W
x
V

T
x
Q

M

L

A

A

C

P

G

I

S

T

G

A

R

D

L

V

L

M

A

G

D

E

L

M

M

L

A

V

K

T

-

G

K

R

T

T

P

P

Q

E

I

V

S

-

A

A

E

K

G

A

L

F

D

A

I

V

S

D

R

R

Y

F

binding flavin-adenine dinucleotide: A174 (≠ D228), A203 (≠ L253), W206 (≠ Y256), G226 (= G275), G306 (= G354), R308 (= R356), S333 (≠ G381), R334 (≠ T382), I335 (≠ L383), G336 (= G384), V337 (≠ W385), Q338 (≠ T386)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
29% identity, 35% coverage: 246:399/434 of query aligns to 195:346/369 of S5FMM4

query
sites
S5FMM4

A

A

D

E

R

H

Y

A

V

V

L

I

A

A

L
x
S

G

G

S

V

Y

W

S

S

P

G

K

A

L

L

L

F

K

K

P

Q

L

I

G

G

I

L

K

D

A

K

P

R

V

F

Y

Y

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

V

V

P

-

I

-

T

W

N

N

P

D

A

G

M

I

A

S

P

L

T

T

S

R

T

T

I

L

L

Y

D

H

E

D

T

H

Y

C

K

Y

V

I

A

-

I

V

T

P

R

R

F

H

D

S

T

G

R

R

I

L

R

V

V

V

G

G

G

-

M

-

A

A

E

T

I

M

A

K

G

P

F

G

D

D

L

W

S

N

L

E

N

Q

P

P

R

E

R

L

R

G

E

G

T

I

L

E

E

E

M

L

I

I

-

R

-

K

V

A

N

K

D

S

L

M

Y

L

P

P

Q

G

G

I

G

E

D

S

L

M

A

K

E

I

A

D

S

Q

F

C

W

W

T

A

G

G

L

L

R

R

P

P

T

E

T

T

P

G

D

D

G

G

T

N

P

P

I

Y

V

I

G

G

A

R

T

H

P

P

F

E

K

N

N

D

-

R

L

I

F

L

L

F

N

A

T

A

G

G

H

H

G

F

T

R

L

N

G

G

W
x
I

T

L

M

L

A

A

C

P

G

A

S

T

G

G

R

E

L

M

L
x
M

A

A

D

D

L

M

M

I

S202 (≠ L253) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ W385) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ L395) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.

1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
23% identity, 62% coverage: 136:405/434 of query aligns to 105:377/402 of 1vrqB

query
sites
1vrqB

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

I

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

F

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

T

R

P

A

E

G

Q

I

V

A

K

R

E

V

V

E

C

P

P

A

I

L

I

A

N

S

T

V

G

T

D

D

N

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

Y

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D

K

C
x
H

Q

D

I

H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q

C

D

E

I
x
V

Q

T

R

G

L

F

D

L

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

L

A

A

D

G

R

K

Y

V

V

A

L

L

A
|
A

L
x
G

G

A

S

G

Y
x
H

S

S

P

S

K

V

L

L

L

A

K

E

P

L

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L
|
L

K

Q

G

-

Y

-

S

A

L

L

T
x
V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I

M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T

H

R

V

F

Y

D

V

T

S

R

Q

I

A

R

H

V

K

G

G

G
x
E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

E

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

R

Y

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

H

K

D

T

E

P

P

Q

H

active site: G326 (= G354), K358 (≠ T386)
binding n,n-dimethylglycine: K358 (≠ T386)
binding flavin-adenine dinucleotide: V196 (≠ I224), A224 (= A252), G225 (≠ L253), H228 (≠ Y256), L247 (= L273), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
binding flavin mononucleotide: H172 (≠ C200), V251 (≠ T279), E279 (≠ G313), R322 (≠ S350), W324 (= W352)

Sites not aligning to the query:

active site: 62, 65, 66
binding n,n-dimethylglycine: 65, 67, 69, 401
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63

3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
23% identity, 62% coverage: 136:405/434 of query aligns to 105:377/404 of 3ad8B

query
sites
3ad8B

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

I

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

F

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

T

R

P

A

E

G

Q

I

V

A

K

R

E

V

V

E

C

P

P

A

I

L

I

A

N

S

T

V

G

T

D

D

N

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

Y

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D

K

C
x
H

Q

D

I

H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q

C

D

E

I
x
V

Q

T

R

G

L

F

D

L

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

L

A

A

D

G

R

K

Y

V

V

A

L

L

A

A

L
x
G

G
x
A

S

G

Y
x
H

S

S

P

S

K

V

L

L

L

A

K

E

P

L

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L
|
L

K

Q

G

-

Y

-

S

A

L

L

T
x
V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I
x
M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T

H

R

V

F
x
Y

D

V

T

S

R

Q

I

A

R

H

V

K

G

G

G
x
E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

E

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

R

Y

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

H

K

D

T

E

P

P

Q

H

active site: G326 (= G354), K358 (≠ T386)
binding flavin-adenine dinucleotide: V196 (≠ I224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (= L273), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
binding flavin mononucleotide: H172 (≠ C200), V251 (≠ T279), E279 (≠ G313), R322 (≠ S350), W324 (= W352)
binding pyrrole-2-carboxylate: M264 (≠ I293), Y271 (≠ F305), T354 (= T382), K358 (≠ T386)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding pyrrole-2-carboxylate: 65, 67, 69, 402

3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
23% identity, 62% coverage: 136:405/434 of query aligns to 105:377/404 of 3ad7B

query
sites
3ad7B

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

I

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

F

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

T

R

P

A

E

G

Q

I

V

A

K

R

E

V

V

E

C

P

P

A

I

L

I

A

N

S

T

V

G

T

D

D

N

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

Y

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D

K

C
x
H

Q

D

I

H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q

C

D

E

I
x
V

Q

T

R

G

L

F

D

L

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

L

A

A

D

G

R

K

Y

V

V

A

L

L

A

A

L
x
G

G
x
A

S

G

Y
x
H

S

S

P

S

K

V

L

L

L

A

K

E

P

L

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L
|
L

K

Q

G

-

Y

-

S

A

L

L

T
x
V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I
x
M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T

H

R

V

F
x
Y

D

V

T

S

R

Q

I

A

R

H

V
x
K

G

G

G
x
E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

E

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

R

Y

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

H

K

D

T

E

P

P

Q

H

active site: G326 (= G354), K358 (≠ T386)
binding flavin-adenine dinucleotide: V196 (≠ I224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (= L273), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
binding flavin mononucleotide: H172 (≠ C200), V251 (≠ T279), K277 (≠ V311), E279 (≠ G313), R322 (≠ S350), W324 (= W352)
binding [methylthio]acetate: M264 (≠ I293), Y271 (≠ F305), T354 (= T382), K358 (≠ T386)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding [methylthio]acetate: 67, 69, 402

Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
23% identity, 62% coverage: 136:405/434 of query aligns to 106:378/405 of Q50LF2

query
sites
Q50LF2

G

G

T

V

T

L

Q

N

L

L

F

A

R

H

T

T

Q

L

A

G

Q

D

L

V

D

R

G

E

A

S

A

I

K

R

D

R

I

V

A

E

V

A

L

N

K

K

E

F

S

N

G

G

V

V

P

D

F

A

E

E

L

W

L

L

D

T

R

P

A

E

G

Q

I

V

A

K

R

E

V

V

E

C

P

P

A

I

L

I

A

N

S

T

V

G

T

D

D

N

I

I

-

R

-

Y

-

P

L

V

A

M

G

G

A

A

L

T

R

Y

L

Q

P

P

N

R

D

A

Q

G

T

I

G

A

D
x
K

C
x
H

Q

D

I

H

F

V

T

A

S

W

R

A

L

F

A

A

E

R

M

K

A

A

V

N

K

E

L

M

G

G

V

V

E

D

F

I

R

I

F

Q

D

N

Q

C

D

E

I
x
V

Q

T

R

G

L

F

D

L

Y

K

A

D

G

G

D

E

R

K

I

V

N

T

G

G

V

V

W

K

I

T

D

T

G

R

K

G

L

T

E

I

T

L

A

A

D

G

R

K

Y

V

V

A

L

L

A

A

L

G

G

A

S

G

Y

H

S

S

P

S

K

V

L

L

L

A

K

E

P

L

L

A

G

G

I

F

K

E

A

L

P

P

V

I

-

Q

-

S

Y

H

P

P

L

L

K

Q

G

-

Y

-

S

A

L

L

T

V

V

S

P

E

I

L

T

F

N

E

P

P

A

-

M

V

A

H

P

P

T

T

S

V

T

V

I

M

L

S

D

N

E

H

T

-

Y

-

K

-

V

-

A

-

I

I

T
x
H

R

V

F
x
Y

D

V

T

S

R

Q

I

A

R

H

V

K

G

G

G

E

M

L

A

V

E

M

I

G

A

A

G

G

F

I

D

D

L

S

S

Y

L

N

N

G

P

Y

R

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

I

I

V

E

N

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

Q

I

G

F

G

A

D

R

L

A

A

H

E

V

A

L

S

R

F

T

W

W

T

G

G

G

L

I

R

V

P

D

T

T

P

M

D

D

G

A

T

S

P

P

I

I

V

I

G

S

A

K

T

T

P

P

F

I

K

Q

N

N

L

L

F

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T

T

L

G

G
|
G

W

F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

R

Y

L

T

L

L

A

A

D

H

L

T

M

I

A

A

K

H

K

D

T

E

P

P

Q

H

K172 (≠ D199) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
H173 (≠ C200) modified: Tele-8alpha-FMN histidine
V197 (≠ I224) binding
H270 (≠ T303) mutation to A: 10-fold decrease in catalytic efficiency.
Y272 (≠ F305) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
G354 (= G381) binding
G357 (= G384) binding
K359 (≠ T386) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.

Sites not aligning to the query:

31 binding
32 binding
53 binding
61 binding
62 binding
66 binding
68 binding

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
27% identity, 36% coverage: 246:402/434 of query aligns to 195:349/369 of O31616

query
sites
O31616

A

A

D

N

R

H

Y

V

V

V

L

V

A

A

L

S

G

G

S

V

Y

W

S

S

P

G

K

M

L

F

K

K

P

Q

L

L

G

G

I

L

K

N

A

N

P

A

V

F

Y

L

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

V

V

P

W

I

N

T

D

N

D

P

-

A

-

M

-

A

I

P

P

T

L

S

T

T

K

I

T

L

L

D

Y

E

H

T

D

Y
x
H

K

C

V

Y

A

I

I

V

T

P

R

R

F

K

D

S

T

G

R

R

I

L

R

V

V

V

G

G

G

A

M

T

A

M

E

K

I

P

A

G

G

D

F

W

D

S

L

E

S

T

L

P

N

D

P

L

R

G

R

L

E

E

T

S

L

V

E

M

M

K

I

K

V

A

N

K

D

T

L

M

Y

L

P

P

Q

A

G

I

G

Q

D

N

L

M

A

K

E

V

A

D

S

R

F

F

W

W

T

A

G

G

L

L

R
|
R

P

P

T

G

T

T

P

K

D

D

G

G

T

K

P

P

I

Y

V

I

G

G

A

R

T

H

P

P

F

E

K

D

N

S

-

R

L

I

F

L

L

F

N

A

T

A

G

G

H
|
H

G
x
F

T
x
R

L
x
N

G
|
G

W
x
I

T
x
L

M

L

A

A

C

P

G

A

S

T

G

G

R

A

L

L

L

I

A

S

D

D

L

L

M

I

A

M

K

N

K

K

H244 (≠ Y298) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R356) binding
327:333 (vs. 380:386, 29% identical) binding
R329 (≠ T382) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
174 binding

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
27% identity, 39% coverage: 246:415/434 of query aligns to 195:363/364 of 3if9A

query
sites
3if9A

A

A

D

N

R

H

Y

V

V

V

L

V

A

A

L
x
S

G
|
G

S

V

Y
x
W

S

S

P

G

K

M

L
x
F

L

F

K

K

P

Q

L

L

G

G

I

L

K

N

A

N

P

A

V

F

Y

L

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

V

V

P

-

I

-

T

W

N

N

P

D

A

D

M

I

A

P

P

L

T

T

S

K

T

T

I

L

L

Y

D

H

E

D

T

A

Y

C

K
x
Y

V

I

A

-

I

V

T

P

R

R

F

K

D

S

T

G

R

R

I

L

R

V

V

V

G

G

G

A

M

T

A

M

E

K

I

P

A

G

G

D

F

W

D

S

L

E

S

T

L

P

N

D

P

L

R

G

R

L

E

E

T

S

L

V

E

M

M

K

I

K

V

A

N

K

D

T

L

M

Y

L

P

P

Q

A

G

I

G

Q

D

N

L

M

A

K

E

V

A

D

S

R

F

F

W

W

T

A

G
|
G

L

L

R
|
R

P

P

T

G

T

T

P

K

D

D

G

G

T

K

P

P

I

Y

V

I

G

G

A

R

T

H

P

P

F

E

K

D

N

S

-

R

L

I

F

L

L

F

N

A

T

A

G

G

H
|
H

G
x
F

T
x
R

L
x
N

G
|
G

W
x
I

T

L

M

L

A

A

C

P

G

A

S

T

G

G

R

A

L

L

L

I

A

S

D

D

L

L

-

I

M

M

A

N

K

K

K

E

T

V

P

N

Q

Q

I

D

S

W

A

L

E

H

G

A

L

F

D

R

I

I

S

D

R

R

binding flavin-adenine dinucleotide: S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (≠ L260), G300 (= G354), R302 (= R356), H327 (= H380), F328 (≠ G381), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
binding glycolic acid: Y246 (≠ K299), R302 (= R356), R329 (≠ T382)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49, 173, 174

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
27% identity, 39% coverage: 246:415/434 of query aligns to 195:363/364 of 1ng3A

query
sites
1ng3A

A

A

D

N

R

H

Y

V

V

V

L

V

A

A

L
x
S

G
|
G

S

V

Y
x
W

S

S

P

G

K

M

L
x
F

L

F

K

K

P

Q

L

L

G

G

I

L

K

N

A

N

P

A

V

F

Y

L

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

V

V

P

W

I

N

T

D

N

D

P

-

A

-

M

-

A

I

P

P

T

L

S

T

T

K

I

T

L

L

D

Y

E

H

T

D

Y

H

K

C

V
x
Y

A

I

I

V

T

P

R

R

F

K

D

S

T

G

R
|
R

I

L

R

V

V

V

G

G

G

A

M

T

A

M

E

K

I

P

A

G

G

D

F

W

D

S

L

E

S

T

L

P

N

D

P

L

R

G

R

L

E

E

T

S

L

V

E

M

M

K

I

K

V

A

N

K

D

T

L

M

Y

L

P

P

Q

P

G

I

G

Q

D

N

L

M

A

K

E

V

A

D

S

R

F

F

W

W

T

A

G
|
G

L

L

R
|
R

P

P

T

G

T

T

P

K

D

D

G

G

T

K

P

P

I

Y

V

I

G

G

A

R

T

H

P

P

F

E

K

D

N

S

-

R

L

I

F

L

L

F

N

A

T

A

G

G

H
|
H

G

F

T
x
R

L
x
N

G
|
G

W
x
I

T

L

M

L

A

A

C

P

G

A

S

T

G

G

R

A

L

L

L

I

A

S

D

D

L

L

-

I

M

M

A

N

K

K

K

E

T

V

P

N

Q

Q

I

D

S

W

A

L

E

H

G

A

L

F

D

R

I

I

S

D

R

R

binding acetylamino-acetic acid: Y246 (≠ V300), R302 (= R356), R329 (≠ T382)
binding flavin-adenine dinucleotide: S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (≠ L260), G300 (= G354), R302 (= R356), H327 (= H380), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
binding phosphate ion: R254 (= R308)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49, 174
binding phosphate ion: 89

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
20% identity, 75% coverage: 93:416/434 of query aligns to 44:368/374 of 1y56B

query
sites
1y56B

L
x
F

R
|
R

N
x
C

C
x
G

T
|
T

A
x
G

S

I

R

R

Y

Q

A

Q

V

F

N

N

K

D

E

E

R

A

M

N

V

V

R

R

L

V

S

M

E

K

Y

R

S

S

R

V

D

E

C

L

L

W

D

K

E

K

L

Y

R

S

A

E

E

E

T

Y

G

G

I

F

A

S

Y

F

E

-

G

-

R

K

T

Q

L

T

G

G

T

Y

T

L

Q

F

L

L

F

L

R

Y

T

D

Q

D

A

E

Q

E

L

V

D

K

G

T

A

F

A

K

K

R

D

N

I

I

A

E

V

I

L

Q

K

N

E

K

S

F

G

G

V

V

P

P

F

T

E

K

L

L

L

I

D

T

R

P

A

E

G

E

I

A

A

K

R

E

V

I

E

V

P

P

A

L

L

L

A

-

S

D

V

I

T

S

D

E

I

V

L

I

A

A

G

A

A

S

L

W

R

N

L

-

P

P

N

T

D

D

Q

G

T

K

G

A

D

D

C

P

Q

F

I

E

F

A

T

T

S

T

R

A

L

F

A

A

E

V

M

K

A

A

V

K

K

E

L

Y

G

G

V

A

E

K

F

L

R

L

F

E

D

Y

Q

T

D
x
E

I
x
V

Q

K

R

G

L

F

D

L

Y

I

A

E

G

N

D

N

R

E

I

I

N

K

G

G

V

V

W

K

I

T

D

N

G

K

K

G

L

I

E

I

T

K

A

T

D

G

R

I

Y

V

V

V

L

N

A

A

L
x
T

G
x
N

S

A

Y
x
W

S

A

P

N

K

L

L

I

L

N

K

A

P

M

L

A

G

G

I

I

K

K

A

T

P

K

V

I

Y

-

P

P

L

I

K

E

G

P

Y

Y

S

K

L
x
H

T

Q

V

A

P

V

I

I

T

T

N

Q

P

P

A

I

M

K

A

R

P

G

T

T

S

I

T

N

I
x
P

L

M

D

V

E

I

T

S

Y

F

K

K

-

Y

-

G

-

H

-

A

-

Y

V

L

A

T

I

Q

T

T

R

F

F

H

D

G

T

G

R

I

I
|
I

R

G

V

G

G

I

G

G

M

Y

A

E

E

I

I

G

A

P

G

T

F

Y

D

D

L

L

S

T

L

P

N

T

P

Y

R

E

R

F

R

L

E

R

T

E

L

V

E

S

M

Y

I

Y

V

F

N

T

D

K

L

I

Y

I

P

P

Q

A

G

L

G

K

D

N

L

L

A

L

E

I

A

L

S
x
R

F

T

W
|
W

T

A

G
|
G

L
x
Y

R
x
Y

P

A

T

K

T

T

P

P

D

D

G

S

T

N

P

P

I

A

V

I

G

G

-

R

A

I

T

E

P

E

F

L

K

N

N

D

L

Y

F

Y

L

I

N

A

T

A

G

G

H

F

G

S

T
x
G

L
x
H

G
|
G

W
x
F

T
x
M

M

M

A

A

C

P

G

A

S

V

G

G

R

E

L

M

L

V

A

A

D

E

L

L

M

I

A

T

K

K

K

G

T

K

P

T

Q

K

I

L

S

P

A

V

E

E

G

W

L

Y

D

D

I

P

S

Y

R

R

Y

F

active site: F44 (≠ L93), G47 (≠ C96), T48 (= T97), H224 (≠ L278), P239 (≠ I293), G305 (= G354), M338 (≠ T386)
binding flavin-adenine dinucleotide: R45 (= R94), C46 (≠ N95), G47 (≠ C96), G49 (≠ A98), E170 (≠ D223), V171 (≠ I224), T200 (≠ L253), N201 (≠ G254), W203 (≠ Y256), G305 (= G354), Y306 (≠ L355), Y307 (≠ R356), G334 (≠ T382), H335 (≠ L383), G336 (= G384), F337 (≠ W385), M338 (≠ T386)
binding flavin mononucleotide: F44 (≠ L93), R45 (= R94), I260 (= I309), R301 (≠ S350), W303 (= W352)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 14, 15, 33, 34, 35, 42, 43

4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
46% identity, 11% coverage: 3:50/434 of query aligns to 6:53/384 of 4x9mA

query
sites
4x9mA

V

V

L

L

V

I

L

V

G
|
G

S

G

G
|
G

V

V

I
|
I

G

G

T

C

A

A

S

T

A

A

Y

Y

Y

E

L

L

A

S

R

Q

A

Y

G

K

F

L

E

K

V

V

T

T

V

L

V

V

D
x
E

R
x
K

Q

H

P

H

A

Y

A

L

A

A

M

Q

E
|
E

T
|
T

S
|
S

F

H

A
|
A

N
|
N

A
x
S

G

G

Q
x
V

V

I

S
x
H

P

T

G

G

binding flavin-adenine dinucleotide: G10 (= G7), G12 (= G9), I14 (= I11), E33 (≠ D30), K34 (≠ R31), E41 (= E38), T42 (= T39), S43 (= S40), A45 (= A42), N46 (= N43), S47 (≠ A44), V49 (≠ Q46), H51 (≠ S48)
binding sn-glycerol-3-phosphate: S47 (≠ A44), H51 (≠ S48)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 176, 177, 209, 210, 212, 234, 319, 320, 346, 347, 348, 349, 350, 351, 352
binding sn-glycerol-3-phosphate: 258, 259, 320, 348

Query Sequence

>AO353_09155 FitnessBrowser__pseudo3_N2E3:AO353_09155
MRVLVLGSGVIGTASAYYLARAGFEVTVVDRQPAAAMETSFANAGQVSPGYASPWAAPGV
PLKAIKWLLQRHAPLAIKATADIDQYLWMAQMLRNCTASRYAVNKERMVRLSEYSRDCLD
ELRAETGIAYEGRTLGTTQLFRTQAQLDGAAKDIAVLKESGVPFELLDRAGIARVEPALA
SVTDILAGALRLPNDQTGDCQIFTSRLAEMAVKLGVEFRFDQDIQRLDYAGDRINGVWID
GKLETADRYVLALGSYSPKLLKPLGIKAPVYPLKGYSLTVPITNPAMAPTSTILDETYKV
AITRFDTRIRVGGMAEIAGFDLSLNPRRRETLEMIVNDLYPQGGDLAEASFWTGLRPTTP
DGTPIVGATPFKNLFLNTGHGTLGWTMACGSGRLLADLMAKKTPQISAEGLDISRYGNTP
KESEKHVNPAPAHQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory