SitesBLAST
Comparing AO353_19350 FitnessBrowser__pseudo3_N2E3:AO353_19350 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2xuaH Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
30% identity, 99% coverage: 1:268/270 of query aligns to 1:261/261 of 2xuaH
4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/274 of 4uhdA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding acetate ion: G33 (≠ S27), F34 (≠ Y28), S100 (= S94), Y104 (≠ M98), R138 (≠ Y131), H249 (= H248)
- binding magnesium ion: A233 (= A233), I236 (= I236), S239 (≠ C238)
4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/272 of 4uheA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding d-malate: F34 (≠ Y28), S100 (= S94), M101 (≠ V95), Y104 (≠ M98), R138 (≠ Y131), H249 (= H248)
4uhfA Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/278 of 4uhfA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding butanoic acid: G33 (≠ S27), F34 (≠ Y28), S100 (= S94), H249 (= H248)
8agsAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 8:287/298 of 8agsAAA
5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments (see paper)
26% identity, 98% coverage: 3:266/270 of query aligns to 8:287/290 of 5ng7B
8agpAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 7:286/297 of 8agpAAA
- binding (1~{S},2~{S},4~{R})-2-chloranyl-1-methyl-4-prop-1-en-2-yl-cyclohexan-1-ol: F32 (≠ Y28), P33 (vs. gap), D99 (≠ S94), W100 (≠ V95), I103 (≠ M98), Y130 (= Y120), Y146 (≠ F133), Y146 (≠ F133), M171 (≠ I156), L211 (≠ F196), H268 (= H248), W269 (≠ I249)
8agnAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 6:285/287 of 8agnAAA
8agmAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 6:285/287 of 8agmAAA
3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog (see paper)
28% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3ia2A
- active site: W28 (≠ Y28), S94 (= S94), M95 (≠ V95), G119 (≠ D118), D222 (= D221), H251 (= H248)
- binding (2R)-butane-2-sulfonate: W28 (≠ Y28), S94 (= S94), M95 (≠ V95), F198 (= F196), I224 (≠ P223), H251 (= H248)
P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 5 papers)
28% identity, 98% coverage: 3:266/270 of query aligns to 4:270/272 of P22862
- W29 (≠ Y28) binding acetate
- L30 (= L29) mutation to I: 125-fold increase in catalytic efficiency for perhydrolase activity with acetic acid as substrate. 2-fold decrease in catalytic efficiency for perhydrolase activity with ethyl acetate as substrate. 1.5-fold increase in catalytic efficiency for hydrolase activity with ethyl acetate as substrate. 2.4-fold increase in kcat for hydrolysis of peracetic acid.; mutation to P: Shows faster acetyl-enzyme formation. Tenfold more efficient at hydrolysis than perhydrolysis with methyl acetate as substrate. 3-fold decrease in catalytic efficiency for hydrolase activity with methyl acetate as substrate. 15-fold decrease in catalytic efficiency for perhydrolase activity with methyl acetate as substrate (PubMed:22618813). 100-fold decrease in hydrolase activity with 4-nitrophenyl acetate as substrate. 28-fold increase in perhydrolase activity with acetate as substrate (PubMed:15803517). 100-fold increase in catalytic efficiency with acetic acid as substrate. 50-fold increase in catalytic efficiency with acetic acid as substrate; when associated with H-58 (PubMed:20112920).
- F58 (≠ H56) mutation to H: 50-fold increase in catalytic efficiency with acetic acid as substrate; when associated with P-30.
- Y70 (≠ L69) mutation to M: Does not affect esterase and perhydrolase activities.
- M96 (≠ V95) binding acetate; mutation to T: 4-fold decrease in esterase activity. Loss of perhydrolase activity.
- D100 (≠ W99) mutation to E: Small decrease in esterase and perhydrolase activities.
- T123 (≠ V121) mutation to P: Does not affect esterase and perhydrolase activities.
- F228 (≠ P226) mutation to I: 3-fold increase in esterase activity. No change in perhydrolase activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
8pi1B Bicyclic incypro pseudomonas fluorescens esterase (see paper)
27% identity, 98% coverage: 3:266/270 of query aligns to 3:269/276 of 8pi1B
Sites not aligning to the query:
3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase (see paper)
27% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3hi4A
3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase (see paper)
27% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3heaA
- active site: W28 (≠ Y28), S94 (= S94), M95 (≠ V95), L118 (≠ M117), G119 (≠ D118), D222 (= D221), H251 (= H248)
- binding ethyl acetate: G27 (≠ S27), W28 (≠ Y28), S94 (= S94), M95 (≠ V95), H251 (= H248)
7p4kA Soluble epoxide hydrolase in complex with fl217 (see paper)
29% identity, 51% coverage: 11:149/270 of query aligns to 22:163/313 of 7p4kA
Sites not aligning to the query:
8zblA Bifunctional epoxide hydrolase 2 (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 249:369/546 of 8zblA
- binding 1-[[(2~{S},4~{a}~{S},6~{a}~{R},6~{a}~{S},6~{b}~{R},8~{a}~{R},10~{S},12~{a}~{S},14~{b}~{S})-10-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2,4~{a},6~{a},6~{b},9,9,12~{a}-heptamethyl-13-oxidanylidene-3,4,5,6,6~{a},7,8,8~{a},10,11,12,14~{b}-dodecahydro-1~{H}-picen-2-yl]methyl]-3-(oxan-4-ylmethyl)urea: F266 (≠ Y28), D334 (≠ S94), W335 (≠ V95), M338 (= M98)
Sites not aligning to the query:
- binding 1-[[(2~{S},4~{a}~{S},6~{a}~{R},6~{a}~{S},6~{b}~{R},8~{a}~{R},10~{S},12~{a}~{S},14~{b}~{S})-10-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2,4~{a},6~{a},6~{b},9,9,12~{a}-heptamethyl-13-oxidanylidene-3,4,5,6,6~{a},7,8,8~{a},10,11,12,14~{b}-dodecahydro-1~{H}-picen-2-yl]methyl]-3-(oxan-4-ylmethyl)urea: 379, 382, 407, 464, 495, 496, 522, 523
- binding magnesium ion: 8, 10, 184
8qn0A Soluble epoxide hydrolase in complex with rk3 (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 25:145/322 of 8qn0A
Sites not aligning to the query:
- binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-5-(4-methylphenyl)sulfonyl-1,3,3~{a},4,6,6~{a}-hexahydropyrrolo[3,4-c]pyrrole-2-carboxamide: 158, 194, 241, 273, 274, 299
8qzdA Soluble epoxide hydrolase in complex with epoxykinin (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 23:143/320 of 8qzdA
Sites not aligning to the query:
- binding 2-[5-bromanyl-3-[2,2,2-tris(fluoranyl)ethanoyl]indol-1-yl]-N-cycloheptyl-ethanamide: 156, 160, 181, 192, 201, 239, 271, 272, 297
8qmzA Soluble epoxide hydrolase in complex with rk4 (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 23:143/320 of 8qmzA
Sites not aligning to the query:
- binding (3~{a}~{R},6~{a}~{S})-~{N}-[(2,4-dichlorophenyl)methyl]-2-(4-methylphenyl)sulfonyl-3,3~{a},4,5,6,6~{a}-hexahydro-1~{H}-cyclopenta[c]pyrrole-5-carboxamide: 156, 157, 192, 239, 271, 272, 297
7a7gA Soluble epoxide hydrolase in complex with tk90 (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 22:142/317 of 7a7gA
Sites not aligning to the query:
Query Sequence
>AO353_19350 FitnessBrowser__pseudo3_N2E3:AO353_19350
MPFATIDGQLLHYVDQGTGPVVLLAGSYLWDQAMWAPQIVALSQHHRVIAVDLWGHGESG
PLPEGMTSLDDQARQVLALLDHLDIDRVTLVGLSVGGMWGVRLALSAPQRLNGLVLMDTY
VGVEPELTRQYYFSLFKQIEDSGVISPQLLDIVVPIFFRPGIDPQSALYQDFRARLAALP
PERLRESIVPMGRITFGRDDLLPRLGELNPETTLLMCGDQDKPRPPLETREMAELIGCPY
LLVPEAGHISNLENPEFVTKALLKFLAERT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory