SitesBLAST
Comparing AO353_19350 FitnessBrowser__pseudo3_N2E3:AO353_19350 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2xuaH Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
30% identity, 99% coverage: 1:268/270 of query aligns to 1:261/261 of 2xuaH
- active site: L32 (≠ Y28), M98 (≠ V95), D214 (= D221), H241 (= H248)
- binding laevulinic acid: L32 (≠ Y28), W132 (≠ Y132), R135 (≠ L135), V151 (= V153), R154 (≠ I156), W155 (≠ F157), Y183 (≠ F196), A216 (≠ P223)
4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/274 of 4uhdA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding acetate ion: G33 (≠ S27), F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), Y104 (≠ M98), R138 (≠ Y131), M196 (= M191), H249 (= H248)
- binding magnesium ion: A233 (= A233), I236 (= I236), S239 (≠ C238)
4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/272 of 4uheA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding d-malate: G33 (≠ S27), F34 (≠ Y28), S100 (= S94), M101 (≠ V95), Y104 (≠ M98), R138 (≠ Y131), M196 (= M191), I223 (≠ P223), H249 (= H248)
4uhfA Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
30% identity, 95% coverage: 11:266/270 of query aligns to 17:267/278 of 4uhfA
- active site: F34 (≠ Y28), L99 (= L93), S100 (= S94), M101 (≠ V95), D124 (= D118), E164 (≠ P155), D221 (= D221), H249 (= H248), L250 (≠ I249)
- binding butanoic acid: G33 (≠ S27), F34 (≠ Y28), S100 (= S94), H249 (= H248), L250 (≠ I249)
8agsAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 8:287/298 of 8agsAAA
5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments (see paper)
26% identity, 98% coverage: 3:266/270 of query aligns to 8:287/290 of 5ng7B
8agpAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 7:286/297 of 8agpAAA
- binding (1~{S},2~{S},4~{R})-2-chloranyl-1-methyl-4-prop-1-en-2-yl-cyclohexan-1-ol: F32 (≠ Y28), P33 (vs. gap), D99 (≠ S94), D99 (≠ S94), W100 (≠ V95), I103 (≠ M98), Y130 (= Y120), W145 (≠ Y132), Y146 (≠ F133), Y146 (≠ F133), F150 (≠ K137), M171 (≠ I156), S175 (≠ P160), Y207 (≠ G192), L211 (≠ F196), I242 (≠ R224), H268 (= H248), W269 (≠ I249)
8agnAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 6:285/287 of 8agnAAA
- binding (1R,6S)-7-oxabicyclo[4.1.0]heptane: F31 (≠ Y28), D98 (≠ S94), W99 (≠ V95), I102 (≠ M98), P124 (vs. gap), Y129 (= Y120), Y145 (≠ F133), M170 (≠ I156), L171 (≠ F157), S174 (≠ P160), L210 (≠ F196), I241 (≠ R224), W268 (≠ I249)
8agmAAA Alpha/beta epoxide hydrolase
26% identity, 98% coverage: 3:266/270 of query aligns to 6:285/287 of 8agmAAA
- binding d-limonene 1,2-epoxide: D98 (≠ S94), W99 (≠ V95), I102 (≠ M98), P124 (vs. gap), Q139 (= Q130), S143 (vs. gap), Y145 (≠ F133), Y145 (≠ F133), F149 (≠ K137), M170 (≠ I156), S174 (≠ P160), L210 (≠ F196), I241 (≠ R224), W268 (≠ I249)
3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog (see paper)
28% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3ia2A
- active site: W28 (≠ Y28), S94 (= S94), M95 (≠ V95), G119 (≠ D118), D222 (= D221), H251 (= H248)
- binding (2R)-butane-2-sulfonate: G27 (≠ S27), W28 (≠ Y28), S94 (= S94), M95 (≠ V95), F125 (vs. gap), F198 (= F196), I224 (≠ P223), H251 (= H248)
P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 5 papers)
28% identity, 98% coverage: 3:266/270 of query aligns to 4:270/272 of P22862
- W29 (≠ Y28) binding
- L30 (= L29) mutation to I: 125-fold increase in catalytic efficiency for perhydrolase activity with acetic acid as substrate. 2-fold decrease in catalytic efficiency for perhydrolase activity with ethyl acetate as substrate. 1.5-fold increase in catalytic efficiency for hydrolase activity with ethyl acetate as substrate. 2.4-fold increase in kcat for hydrolysis of peracetic acid.; mutation to P: Shows faster acetyl-enzyme formation. Tenfold more efficient at hydrolysis than perhydrolysis with methyl acetate as substrate. 3-fold decrease in catalytic efficiency for hydrolase activity with methyl acetate as substrate. 15-fold decrease in catalytic efficiency for perhydrolase activity with methyl acetate as substrate (PubMed:22618813). 100-fold decrease in hydrolase activity with 4-nitrophenyl acetate as substrate. 28-fold increase in perhydrolase activity with acetate as substrate (PubMed:15803517). 100-fold increase in catalytic efficiency with acetic acid as substrate. 50-fold increase in catalytic efficiency with acetic acid as substrate; when associated with H-58 (PubMed:20112920).
- F58 (≠ H56) mutation to H: 50-fold increase in catalytic efficiency with acetic acid as substrate; when associated with P-30.
- Y70 (≠ L69) mutation to M: Does not affect esterase and perhydrolase activities.
- M96 (≠ V95) binding ; mutation to T: 4-fold decrease in esterase activity. Loss of perhydrolase activity.
- D100 (≠ W99) mutation to E: Small decrease in esterase and perhydrolase activities.
- T123 (≠ V121) mutation to P: Does not affect esterase and perhydrolase activities.
- F228 (≠ P226) mutation to I: 3-fold increase in esterase activity. No change in perhydrolase activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase (see paper)
27% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3hi4A
3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase (see paper)
27% identity, 98% coverage: 3:266/270 of query aligns to 3:269/271 of 3heaA
- active site: W28 (≠ Y28), S94 (= S94), M95 (≠ V95), L118 (≠ M117), G119 (≠ D118), D222 (= D221), H251 (= H248)
- binding ethyl acetate: G27 (≠ S27), W28 (≠ Y28), F93 (≠ L93), S94 (= S94), M95 (≠ V95), F198 (= F196), I224 (≠ P223), H251 (= H248)
7p4kA Soluble epoxide hydrolase in complex with fl217 (see paper)
29% identity, 51% coverage: 11:149/270 of query aligns to 22:163/313 of 7p4kA
Sites not aligning to the query:
5fp0A Ligand complex structure of soluble epoxide hydrolase (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 249:369/543 of 5fp0A
Sites not aligning to the query:
- active site: 374, 379, 462, 492, 520
- binding N-cyclopentyl-2-[4-(trifluoromethyl)phenyl]-3H-benzimidazole-4-sulfonamide: 377, 379, 380, 404, 415, 462, 465, 494, 495, 499, 520, 521
6aumA Crystal structure of human soluble epoxide hydrolase complexed with trans-4-[4-(3-trifluoromethoxyphenyl-l-ureido)-cyclohexyloxy]-benzoic acid. (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 249:369/547 of 6aumA
Sites not aligning to the query:
- active site: 377, 382, 465, 495, 523
- binding 4-{[trans-4-({[4-(trifluoromethoxy)phenyl]carbamoyl}amino)cyclohexyl]oxy}benzoic acid: 373, 374, 380, 382, 383, 386, 407, 418, 427, 465, 498, 502, 523
- binding magnesium ion: 8, 10, 184
5am5A Ligand complex structure of soluble epoxide hydrolase (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 249:369/546 of 5am5A
Sites not aligning to the query:
- active site: 377, 382, 465, 495, 523
- binding 5-cyclohexyl-3-propyl-1H-pyridin-2-one: 370, 374, 380, 382, 383, 414, 418, 467, 468, 471, 495, 496, 497, 502, 523
7a7gA Soluble epoxide hydrolase in complex with tk90 (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 22:142/317 of 7a7gA
Sites not aligning to the query:
6yl4A Soluble epoxide hydrolase in complex with 3-((r)-3-(1-hydroxyureido) but-1-yn-1-yl)-n-((s)-3-phenyl-3-(4-trifluoromethoxy)phenyl)propyl) benzamide (see paper)
31% identity, 44% coverage: 11:128/270 of query aligns to 22:142/319 of 6yl4A