SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO353_23370 FitnessBrowser__pseudo3_N2E3:AO353_23370 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00069 Cytochrome c from Guizotia abyssinica (Niger) (Ramtilla)
46% identity, 78% coverage: 25:124/129 of query aligns to 8:107/111 of P00069

query
sites
P00069

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K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

1co6A Crystal structure of ferrocytochrome c2 from rhodopseudomonas viridis (see paper)
44% identity, 78% coverage: 27:127/129 of query aligns to 2:101/107 of 1co6A

query
sites
1co6A

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binding heme c: C13 (= C39), C16 (= C42), H17 (= H43), P29 (= P55), S39 (≠ A65), Y47 (= Y73), S48 (= S74), N51 (≠ M77), W58 (= W84), F63 (≠ L89), I67 (= I93), T77 (= T103), K78 (≠ R104), M79 (= M105), F81 (= F107)

P00053 Cytochrome c from Cannabis sativa (Hemp) (Marijuana)
45% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of P00053

query
sites
P00053

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K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00067 Cytochrome c from Tropaeolum majus (Common nasturtium) (Indian cress) (see paper)
46% identity, 78% coverage: 25:124/129 of query aligns to 8:107/111 of P00067

query
sites
P00067

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K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00071 Cytochrome c from Pastinaca sativa (Wild parsnip) (Anethum pastinaca) (see paper)
45% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of P00071

query
sites
P00071

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K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00015 Cytochrome c, testis-specific from Mus musculus (Mouse) (see paper)
45% identity, 77% coverage: 26:124/129 of query aligns to 2:100/105 of P00015

query
sites
P00015

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G2 (= G26) modified: N-acetylglycine
C15 (= C39) binding covalent
C18 (= C42) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00052 Cytochrome c from Vigna radiata var. radiata (Mung bean) (Phaseolus aureus) (see paper)
44% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of P00052

query
sites
P00052

G

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K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

2aiuA Crystal structure of mouse testicular cytochromE C at 1.6 angstrom (see paper)
45% identity, 77% coverage: 26:124/129 of query aligns to 1:99/104 of 2aiuA

query
sites
2aiuA

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binding protoporphyrin ix containing fe: C14 (= C39), Q16 (≠ G41), C17 (= C42), H18 (= H43), P30 (= P55), L35 (≠ I60), G41 (= G66), Y48 (= Y73), T49 (≠ S74), N52 (≠ M77), W59 (= W84), Y67 (= Y92), L68 (≠ I93), T78 (= T103), K79 (≠ R104), M80 (= M105), F82 (= F107)

P00066 Cytochrome c from Nigella damascena (Love-in-a-mist) (see paper)
43% identity, 78% coverage: 25:124/129 of query aligns to 8:107/111 of P00066

query
sites
P00066

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C22 (= C39) binding covalent
C25 (= C42) binding covalent
K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

Q0DI31 Cytochrome c from Oryza sativa subsp. japonica (Rice) (see paper)
44% identity, 77% coverage: 26:124/129 of query aligns to 10:108/112 of Q0DI31

query
sites
Q0DI31

G

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K81 (= K97) modified: N6,N6,N6-trimethyllysine
K95 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P00051 Cytochrome c from Cucurbita maxima (Pumpkin) (Winter squash) (see paper)
44% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of P00051

query
sites
P00051

G

G

D

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A

A

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L

E

L

A

N

P

P

K
|
K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

P

G

G

I

L

S
x
K

D

K

P

P

E

Q

K

D

I

R

E

A

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00083 Cytochrome c2 from Blastochloris viridis (Rhodopseudomonas viridis) (see 2 papers)
43% identity, 76% coverage: 30:127/129 of query aligns to 25:121/127 of P00083

query
sites
P00083

A
x
S

G
|
G

G
x
E

K
x
Q

L
x
V

F
|
F

N
x
K

R
x
Q

I

-

C
|
C

G
x
L

G
x
V

C
|
C

H
|
H

Q
x
S

V
x
I

G
|
G

D
x
P

S
x
G

A
|
A

R
x
K

G
x
N

S
x
K

F
x
V

G
|
G

P
|
P

Q
x
V

L
|
L

N
|
N

G
|
G

I
x
L

F
|
F

G
|
G

R
|
R

H
|
H

A
x
S

G
|
G

S
x
T

T
x
I

T
x
E

D
x
G

Y
x
F

Q
x
A

Y
|
Y

S
|
S

T
x
D

A
|
A

M
x
N

K
|
K

S
x
N

S
|
S

D
x
G

V
x
I

V
x
T

W
|
W

T
|
T

R
x
E

E
|
E

T
x
V

L
x
F

A
x
R

A
x
E

Y
|
Y

I
|
I

E
x
R

A
x
D

P
|
P

K
|
K

K
x
A

V
x
K

V
x
I

P
|
P

G
|
G

T
|
T

R
x
K

M
|
M

I
|
I

F
|
F

W
x
A

G
|
G

I
x
V

S
x
K

D
|
D

P
x
E

E
x
Q

K
|
K

I
x
V

E
x
S

N
x
D

L
|
L

L
x
I

A
|
A

Y
|
Y

L
x
I

Q
x
K

T
x
Q

F
|
F

Q
x
N

C33 (= C39) binding covalent
C36 (= C42) binding covalent
H37 (= H43) binding axial binding residue
M99 (= M105) binding axial binding residue

Sites not aligning to the query:

1:20 signal peptide
21 modified: Pyrrolidone carboxylic acid
21:127 modified: mature protein, Cytochrome c2

P68519 Cytochrome c from Crotalus viridis viridis (Prairie rattlesnake) (see paper)
41% identity, 77% coverage: 26:124/129 of query aligns to 2:100/105 of P68519

query
sites
P68519

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

N

S

R

M

I

K

C

C

G

G

G

T

C

C

H

H

Q

T

V

V

G

E

D

E

S

G

A

G

R

K

G

H

S

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

F

F

G

G

R

R

H

K

A

T

G

G

S

Q

T

A

T

V

D

G

Y

Y

Q

S

Y

Y

S

T

T

A

A

A

M

N

K

K

S

N

S

K

D

G

V

I

V

I

W

W

T

G

R

D

E

D

T

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

T

G

G

I

L

S

K

D

S

P

K

E

K

K

E

I

R

E

T

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

G2 (= G26) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68518 Cytochrome c from Crotalus atrox (Western diamondback rattlesnake) (see paper)
41% identity, 77% coverage: 26:124/129 of query aligns to 2:100/105 of P68518

query
sites
P68518

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

N

S

R

M

I

K

C

C

G

G

G

T

C

C

H

H

Q

T

V

V

G

E

D

E

S

G

A

G

R

K

G

H

S

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

F

F

G

G

R

R

H

K

A

T

G

G

S

Q

T

A

T

V

D

G

Y

Y

Q

S

Y

Y

S

T

T

A

A

A

M

N

K

K

S

N

S

K

D

G

V

I

V

I

W

W

T

G

R

D

E

D

T

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

T

G

G

I

L

S

K

D

S

P

K

E

K

K

E

I

R

E

T

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

G2 (= G26) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68517 Cytochrome c from Crotalus adamanteus (Eastern diamondback rattlesnake) (see paper)
41% identity, 77% coverage: 26:124/129 of query aligns to 2:100/105 of P68517

query
sites
P68517

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

I

F

F

N

S

R

M

I

K

C

C

G

G

G

T

C

C

H

H

Q

T

V

V

G

E

D

E

S

G

A

G

R

K

G

H

S

K

F

T

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

F

F

G

G

R

R

H

K

A

T

G

G

S

Q

T

A

T

V

D

G

Y

Y

Q

S

Y

Y

S

T

T

A

A

A

M

N

K

K

S

N

S

K

D

G

V

I

V

I

W

W

T

G

R

D

E

D

T

T

L

L

A

M

A

E

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

T

G

G

I

L

S

K

D

S

P

K

E

K

K

E

I

R

E

T

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

G2 (= G26) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ccrA Structure of rice ferricytochromE C at 2.0 angstroms resolution (see paper)
44% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of 1ccrA

query
sites
1ccrA

G

G

D

N

A

P

E

K

A

A

G

G

G

E

K

K

L

I

F

F

N

K

R

T

I
x
K

C
|
C

G

A

G

Q

C
|
C

H
|
H

Q

T

V

V

G

D

D

K

S

G

A

A

R

G

G

H

S

K

F
x
Q

G
|
G

P
|
P

Q

N

L

L

N

N

G

G

I

L

F

F

G

G

R

R

H

Q

A
x
S

G

G

S

T

T

T

T

P

D

G

Y
|
Y

Q

S

Y
|
Y

S
|
S

T

T

A

A

M
x
D

K

K

S

N

S

M

D

A

V

V

V

I

W
|
W

T

E

R

E

E

N

T

T

L

L

A

Y

A

D

Y
|
Y

I

L

E

L

A

N

P

P

K

K

K

K

V

Y

V

I

P

P

G

G

T
|
T

R
x
K

M
|
M

I

V

F
|
F

W

P

G

G

I

L

S

K

D

K

P

P

E

Q

K

E

I

R

E

A

N

D

L

L

L

I

A

S

Y

Y

L

L

Q

K

binding heme c: K21 (≠ I38), C22 (= C39), C25 (= C42), H26 (= H43), Q36 (≠ F53), G37 (= G54), P38 (= P55), S48 (≠ A65), Y54 (= Y71), Y56 (= Y73), S57 (= S74), D60 (≠ M77), W67 (= W84), Y75 (= Y92), T86 (= T103), K87 (≠ R104), M88 (= M105), F90 (= F107)

P00028 Cytochrome c from Entosphenus tridentatus (Pacific lamprey) (Lampetra tridentata) (see paper)
44% identity, 77% coverage: 26:124/129 of query aligns to 2:100/105 of P00028

query
sites
P00028

G
|
G

D

D

A

V

E

E

A

K

G

G

G

K

K

K

L

V

F

F

N

V

R

Q

I

K

C
|
C

G

S

G

Q

C
|
C

H

H

Q

T

V

V

G

E

D

K

S

A

A

G

R

K

G

H

S

K

F

T

G

G

P

P

Q

N

L

L

N

S

G

G

I

L

F

F

G

G

R

R

H

K

A

T

G

G

S

Q

T

A

T

P

D

G

Y

F

Q

S

Y

Y

S

T

T

D

A

A

M

N

K

K

S

S

S

K

D

G

V

I

V

V

W

W

T

N

R

Q

E

E

T

T

L

L

A

F

A

V

Y

Y

I

L

E

E

A

N

P

P

K

K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

I

F

F

W

A

G

G

I

I

S

K

D

K

P

E

E

G

K

E

I

R

E

K

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

G2 (= G26) modified: N-acetylglycine
C15 (= C39) binding covalent
C18 (= C42) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00074 Cytochrome c from Ginkgo biloba (Ginkgo) (Maidenhair tree) (see paper)
43% identity, 77% coverage: 26:124/129 of query aligns to 9:107/113 of P00074

query
sites
P00074

G

G

D

D

A

P

E

K

A

A

G

G

G

E

K

K

L

I

F

F

N

K

R

T

I

K

C
|
C

G

A

G

Z

C
|
C

H

H

Q

T

V

V

G

Z

D

K

S

G

A

A

R

G

G

H

S

K

F

Q

G

G

P

P

Q

N

L

L

N

H

G

G

I

L

F

F

G

G

R

R

H

Q

A

S

G

G

S

T

T

T

T

A

D

G

Y

Y

Q

S

Y

Y

S

S

T

T

A

G

M

N

K

K

S

N

S

K

D

A

V

V

V

N

W

W

T

G

R

Z

E

Z

T

T

L

L

A

Y

A

E

Y

Y

I

L

E

L

A

N

P

P

K
|
K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

P

G

G

I

L

S
x
K

D

K

P

P

E

Z

K

Z

I

R

E

A

N

D

L

L

L

I

A

S

Y

Y

L

L

Q

K

C22 (= C39) binding covalent
C25 (= C42) binding covalent
K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00058 Cytochrome c from Gossypium barbadense (Sea-island cotton) (Egyptian cotton) (see paper)
45% identity, 77% coverage: 26:124/129 of query aligns to 9:107/111 of P00058

query
sites
P00058

G

G

D

B

A

A

E

K

A

A

G

G

G

E

K

K

L

I

F

F

N

K

R

T

I

K

C
|
C

G

A

G

Q

C
|
C

H

H

Q

T

V

V

G

D

D

K

S

G

A

A

R

G

G

H

S

K

F

Q

G

G

P

P

Q

N

L

L

N

N

G

G

I

L

F

F

G

G

R

R

H

Q

A

S

G

G

S

T

T

T

T

A

D

G

Y

Y

Q

S

Y

Y

S

S

T

A

A

A

M

N

K

K

S

N

S

M

D

A

V

V

V

Q

W

W

T

G

R

E

E

N

T

T

L

L

A

Y

A

D

Y

Y

I

L

E

L

A

N

P

P

K
|
K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

P

G

G

I

L

S
x
K

D

K

P

P

E

Q

K

D

I

R

E

A

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

C22 (= C39) binding covalent
C25 (= C42) binding covalent
K80 (= K97) modified: N6,N6,N6-trimethyllysine
K94 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P00070 Cytochrome c from Helianthus annuus (Common sunflower) (see paper)
44% identity, 78% coverage: 25:125/129 of query aligns to 9:109/112 of P00070

query
sites
P00070

A

A

G

G

D

N

A

P

E

T

A

T

G

G

G

E

K

K

L

I

F

F

N

K

R

T

I

K

C
|
C

G

A

G

Q

C
|
C

H

H

Q

T

V

V

G

E

D

K

S

G

A

A

R

G

G

H

S

K

F

Q

G

G

P

P

Q

N

L

L

N

N

G

G

I

L

F

F

G

G

R

R

H

Q

A

S

G

G

S

T

T

T

T

A

D

G

Y

Y

Q

S

Y

Y

S

S

T

A

A

G

M

N

K

K

S

N

S

K

D

A

V

V

V

I

W

W

T

E

R

E

E

N

T

T

L

L

A

Y

A

D

Y

Y

I

L

E

L

A

N

P

P

K
|
K

K

K

V

Y

V

I

P

P

G

G

T

T

R

K

M

M

I

V

F

F

W

P

G

G

I

L

S
x
K

D

K

P

P

E

Q

K

E

I

R

E

A

N

D

L

L

L

I

A

A

Y

Y

L

L

Q

K

T

T

C23 (= C39) binding covalent
C26 (= C42) binding covalent
K81 (= K97) modified: N6,N6,N6-trimethyllysine
K95 (≠ S111) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

Query Sequence

>AO353_23370 FitnessBrowser__pseudo3_N2E3:AO353_23370
LKKAAVLTVALMLNTGVFSTPADAAGDAEAGGKLFNRICGGCHQVGDSARGSFGPQLNGI
FGRHAGSTTDYQYSTAMKSSDVVWTRETLAAYIEAPKKVVPGTRMIFWGISDPEKIENLL
AYLQTFQPQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory