SitesBLAST

I147 (≠ V46) to S: in D2HGA1; severe phenotype; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ H52) to T: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ T71) to Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (= P88) to L: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A105) to V: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (≠ G131) to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity
G233 (= G133) to S: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs374535734

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
109 S → W: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs142050154
127 N → K: in D2HGA1; unknown pathological significance; complete loss of catalytic activity; dbSNP:rs762857195
131 G → V: in D2HGA1; unknown pathological significance; complete loss of catalytic activity
169:521 natural variant: Missing (in D2HGA1; unknown pathological significance)
375 D → Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs267606759
386 binding ; binding ; binding ; R→A: Loss of catalytic activity.
390 binding ; binding ; T→A: Significantly reduced catalytic activity.
399 V → M: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746519212
400:521 natural variant: Missing (in D2HGA1; unknown pathological significance; complete loss of catalytic activity)
401 binding ; binding ; K→A: Loss of catalytic activity.
419 R → H: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs199908032
426 A → T: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs146578303
434 binding ; H→A: Loss of catalytic activity.
436 G → V: slight reduction in catalytic activity
439 N → D: in D2HGA1; mild phenotype; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs121434362
441 binding ; H→A: Loss of catalytic activity.
443 binding ; N→A: Significantly reduced catalytic activity.
444 V → A: in D2HGA1; severe phenotype; unknown pathological significance; significant reduction in catalytic activity; dbSNP:rs121434360
446 A → V: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746956176
475 binding ; E→A: Loss of catalytic activity.
476 binding ; binding ; binding ; H→A: Loss of catalytic activity.
477 G → R: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
30% identity, 38% coverage: 46:177/352 of query aligns to 88:218/465 of 3pm9A

query
sites
3pm9A

V

V

L

I

D

S

T
x
L

R

K

V

R

H

M

R

D

G

K

I

I

V

R

H

E

Y

I

D

D

P

T

T

S

E

S

L

N

V

T

I

I

T

T

A

V

R

E

A

A

G

G

T

A

P

I

L

L

R

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E

R

L

V

L

Q

A

E

A

K

L

A

E

A

A

E

A

V

G

D

Q

R

R

L

L

F

P

P

C

L

E

S

P
x
L

P
x
G

A
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A

F

Q

G

G

D

S

D

-

A
x
C

T
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T

V

I

G
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G

M
x
N

V

L

A
x
S

A
x
T

G

N

L
x
A

S
x
G

G

G

P

T

R

A

P

L

W

A

A

Y

G

G

S
x
L

V

A

R

R

D

D

F

M

V

A

L

L

G

G

T

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E

L

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I

V

S

L

G

A

H

D

G

G

T

R

V

V

L

M

R

N

F

L

G

L

G

S

E

K

V

L

M

K

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

L

L

S

R

R

D

L

L

L

F

A

I

G

G

S

A

F
x
E

G
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G

C

T

L

L

G
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G

V
x
I

I
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I

T

T

E

A

V

A

S

T

L

L

K

K

V

L

L

F

P

P

K

K

P

P

R

R

active site: A149 (≠ L108), L159 (≠ S118)
binding flavin-adenine dinucleotide: L91 (≠ T49), L133 (≠ P91), G134 (≠ P92), A135 (= A93), C139 (≠ A98), T140 (= T99), G142 (= G101), G143 (= G102), N144 (≠ M103), S146 (≠ A105), T147 (≠ A106), A149 (≠ L108), G150 (≠ S109), E200 (≠ F159), G201 (= G160), G204 (= G163), I205 (≠ V164), I206 (= I165)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 69, 70, 71, 72, 73, 74, 75, 76, 79, 80, 423, 424, 460

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
30% identity, 38% coverage: 47:178/352 of query aligns to 258:387/658 of P97275

query
sites
P97275

L

L

D

D

T

T

R

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M

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N

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R

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D

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N

E

N

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A

T

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A

A

G

G

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A

Q

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E

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x
H

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x
D

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x
S

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x
L

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x
E

D
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F

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x
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T

V

V

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G

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W

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A

S

A
x
T

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x
R

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x
A

S
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S

G

G

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N

W

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Y

G

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N

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D

D

F

L

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V

L

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V

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M

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G

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D

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G

G

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S

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x
E

G
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G

C
x
T

L
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L

G
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G

V
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V

I
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I

T

T

E

E

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L

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K

V

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P

K

T

P

P

R

E

H

Y

H300 (≠ C89) mutation to A: Loss of activity.
303:309 (vs. 92:99, 13% identical) binding
T309 (= T99) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
TRAS 316:319 (≠ AGLS 106:109) binding
S367 (= S158) mutation to A: Strongly reduced activity.
368:374 (vs. 159:165, 71% identical) binding

Sites not aligning to the query:

1:58 modified: transit peptide, Peroxisome
234:240 binding
419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
469 L→P: Impaired FAD binding and protein stability. Loss of activity.
515 binding ; R→L: Impaired FAD binding and protein stability. Loss of activity.
576 C→A: No effect on activity.
578 active site, Proton donor/acceptor; Y→F: Loss of activity.
615 H→A: Loss of activity.
615:617 Important for enzyme activity
616 H→A: Loss of activity.
617 H→A: Loss of activity.
654:658 Important for enzyme activity

5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 178:307/557 of 5adzC

query
sites
5adzC

L

L

D

D

T

T

R

S

V

Q

H

M

R

N

G

R

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D

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N

E

N

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A

A

G

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E

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A

Q

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K

A

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A

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P

P
x
D

A
x
S

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x
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T

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G

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W

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x
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A

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S

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V

V

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N

M

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G

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G

G

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S

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x
E

G
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G

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G
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G

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V

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I

T

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E

E

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K

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P

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T

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P

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H

Y

binding flavin-adenine dinucleotide: P222 (= P91), D223 (≠ P92), S224 (≠ A93), S228 (≠ A98), T229 (= T99), G231 (= G101), G232 (= G102), W233 (≠ M103), S235 (≠ A105), T236 (≠ A106), A238 (≠ L108), S239 (= S109), E288 (≠ F159), G289 (= G160), G292 (= G163), V293 (= V164), I294 (= I165)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ P92)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 16, 109, 154, 155, 156, 157, 158, 159, 160, 161, 164, 165, 515, 553
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): 410, 411, 413, 414, 424, 425, 426, 477, 479, 480, 481, 515, 516

6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 178:307/554 of 6gouA

query
sites
6gouA

L

L

D

D

T

T

R

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M

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R

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D

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N

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S

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E

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G

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Y

binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ P92)
binding flavin-adenine dinucleotide: P222 (= P91), D223 (≠ P92), S224 (≠ A93), S228 (≠ A98), T229 (= T99), G231 (= G101), G232 (= G102), W233 (≠ M103), S235 (≠ A105), T236 (≠ A106), A238 (≠ L108), S239 (= S109), E288 (≠ F159), G289 (= G160), G292 (= G163), V293 (= V164), I294 (= I165)

Sites not aligning to the query:

binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: 407, 410, 411, 421, 422, 423, 474, 476, 477, 478, 512, 513
binding flavin-adenine dinucleotide: 16, 109, 154, 155, 156, 157, 158, 159, 160, 161, 164, 165, 512, 550

O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
30% identity, 38% coverage: 47:178/352 of query aligns to 258:387/658 of O00116

query
sites
O00116

L

L

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D

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Y

Sites not aligning to the query:

182 R → Q: in RCDP3; severely reduced protein levels
419 Important for enzyme activity; R → H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411
471 E → K: in RCDP3; severely reduced protein levels
568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214

4bc7B Mammalian alkyldihydroxyacetonephosphate synthase: arg419his mutant (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 164:293/543 of 4bc7B

query
sites
4bc7B

L

L

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S

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x
E

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G

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Y

binding flavin-adenine dinucleotide: P208 (= P91), D209 (≠ P92), S210 (≠ A93), S214 (≠ A98), T215 (= T99), G217 (= G101), G218 (= G102), W219 (≠ M103), S221 (≠ A105), T222 (≠ A106), A224 (≠ L108), S225 (= S109), E274 (≠ F159), G275 (= G160), G278 (= G163), V279 (= V164), I280 (= I165)

Sites not aligning to the query:

binding 1-dodecanol: 400, 410, 411, 412, 463, 467
binding flavin-adenine dinucleotide: 16, 140, 141, 142, 143, 144, 145, 146, 147, 150, 151, 501, 502, 539

5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 164:293/539 of 5ae3A

query
sites
5ae3A

L

L

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K

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P

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T

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G

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S

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P

M

R

K

P

N

W

I

A

Y

G

G

S

N

V

I

R

E

D

D

F

L

V

V

L

V

G

H

T

M

R

K

L

V

I

V

S

T

G

P

H

R

G

G

T

-

V

V

L

I

R

E

F

K

G

S

G

C

E

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

L

I

S

H

R

H

L

F

L

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A

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G

G

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S

F
x
E

G
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G

C

T

L

L

G
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G

V
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V

I
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I

T

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

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P

K

T

P

P

R

E

H

Y

binding flavin-adenine dinucleotide: P208 (= P91), D209 (≠ P92), S210 (≠ A93), S214 (≠ A98), T215 (= T99), G217 (= G101), G218 (= G102), W219 (≠ M103), S221 (≠ A105), T222 (≠ A106), A224 (≠ L108), S225 (= S109), E274 (≠ F159), G275 (= G160), G278 (= G163), V279 (= V164), I280 (= I165)

Sites not aligning to the query:

binding [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 3-methylbutanoate: 45, 48, 49, 53, 325, 327, 331, 334, 349, 386, 387, 389, 393, 396, 400, 407, 408, 443, 447, 461
binding flavin-adenine dinucleotide: 13, 95, 140, 141, 142, 143, 144, 145, 146, 147, 150, 151, 497, 498, 535

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 164:293/539 of 5ae1B

query
sites
5ae1B

L

L

D

D

T

T

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M

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N

G

R

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I

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H

W

Y

V

D

D

P

E

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N

E

N

L

L

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T

I

A

T

H

A

V

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E

A

A

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G

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T

L

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E

E

L

L

L

E

A

R

A

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L

L

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K

A

E

A

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G

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Y

R

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P

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x
D

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x
S

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x
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G

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x
W

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x
S

A
x
T

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x
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S

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G

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N

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Y

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V

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G

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Q

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N

M

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G

Y

P

D

D

L

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L

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S

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x
E

G
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G

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L

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G

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I

T

T

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E

V

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T

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H

Y

binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): D209 (≠ P92)
binding flavin-adenine dinucleotide: P208 (= P91), D209 (≠ P92), S210 (≠ A93), S214 (≠ A98), T215 (= T99), G217 (= G101), G218 (= G102), W219 (≠ M103), S221 (≠ A105), T222 (≠ A106), A224 (≠ L108), S225 (= S109), E274 (≠ F159), G275 (= G160), G278 (= G163), V279 (= V164), I280 (= I165)

Sites not aligning to the query:

binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): 95, 392, 395, 396, 406, 407, 408, 459, 461, 462, 463, 497, 498
binding flavin-adenine dinucleotide: 16, 95, 140, 141, 142, 143, 144, 145, 146, 147, 150, 151, 497, 535

4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 169:298/555 of 4bc9A

query
sites
4bc9A

L

L

D

D

T

T

R

S

V

Q

H

M

R

N

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N

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E

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Y

binding propanenitrile: D214 (≠ P92)
binding flavin-adenine dinucleotide: P213 (= P91), D214 (≠ P92), S215 (≠ A93), S219 (≠ A98), T220 (= T99), G222 (= G101), G223 (= G102), W224 (≠ M103), S226 (≠ A105), T227 (≠ A106), A229 (≠ L108), S230 (= S109), E279 (≠ F159), G280 (= G160), G283 (= G163), V284 (= V164), I285 (= I165)

Sites not aligning to the query:

binding propanenitrile: 475, 513, 514
binding flavin-adenine dinucleotide: 16, 145, 146, 147, 148, 149, 150, 151, 152, 155, 156, 513, 551

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 164:293/542 of 5ae2B

query
sites
5ae2B

L

L

D

D

T

T

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R

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N

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E

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S

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x
E

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G

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H

Y

binding flavin-adenine dinucleotide: P208 (= P91), D209 (≠ P92), S210 (≠ A93), S214 (≠ A98), T215 (= T99), G217 (= G101), G218 (= G102), W219 (≠ M103), S221 (≠ A105), T222 (≠ A106), A224 (≠ L108), S225 (= S109), E274 (≠ F159), G275 (= G160), G278 (= G163), V279 (= V164), I280 (= I165)
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: D209 (≠ P92)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 16, 140, 141, 142, 143, 144, 145, 146, 147, 150, 151, 500, 538
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 395, 396, 399, 409, 410, 411, 462, 464, 465, 466, 500, 501

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 169:298/561 of 5ae2A

query
sites
5ae2A

L

L

D

D

T

T

R

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M

R

N

G

R

I

I

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D

P

E

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N

E

N

L

L

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I

A

T

H

A

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A

A

G

G

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T

L

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E

L

L

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A

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G

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P

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G

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x
W

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x
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x
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S

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Y

G

G

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D

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V

L

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H

R

G

G

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V

V

L

I

R

E

F

K

G

S

G

C

E

Q

V

G

M

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K

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N

M

V

S

A

T

G

G

Y

P

D

D

L

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L

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L

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G

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S

F
x
E

G
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G

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L

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G

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I

T

T

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E

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K

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H

Y

binding flavin-adenine dinucleotide: P213 (= P91), D214 (≠ P92), S215 (≠ A93), S219 (≠ A98), T220 (= T99), G222 (= G101), G223 (= G102), W224 (≠ M103), S226 (≠ A105), T227 (≠ A106), A229 (≠ L108), S230 (= S109), E279 (≠ F159), G280 (= G160), G283 (= G163), V284 (= V164), I285 (= I165)
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: D214 (≠ P92)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 16, 145, 146, 147, 148, 149, 150, 151, 152, 155, 156, 519, 557
binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 414, 415, 417, 418, 428, 429, 430, 481, 483, 484, 485, 519, 520

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
30% identity, 38% coverage: 47:178/352 of query aligns to 164:293/542 of 4bc9B

query
sites
4bc9B

L

L

D

D

T

T

R

S

V

Q

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M

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G

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N

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A

A

G

G

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x
S

A
x
T

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x
A

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S

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G

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I

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Y

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V

L

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V

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N

M

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E

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G

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H

Y

binding propanenitrile: D209 (≠ P92)
binding flavin-adenine dinucleotide: P208 (= P91), D209 (≠ P92), S210 (≠ A93), S214 (≠ A98), T215 (= T99), G217 (= G101), G218 (= G102), W219 (≠ M103), S221 (≠ A105), T222 (≠ A106), A224 (≠ L108), S225 (= S109), E274 (≠ F159), G275 (= G160), G278 (= G163), V279 (= V164), I280 (= I165)

Sites not aligning to the query:

binding propanenitrile: 462, 500, 501
binding flavin-adenine dinucleotide: 16, 95, 140, 141, 142, 143, 144, 145, 146, 147, 150, 151, 500, 538

Query Sequence

>AO356_01095 FitnessBrowser__pseudo5_N2C3_1:AO356_01095
MSREADQDASDLLLEQVSRARANATPLRIQGSNSKAFLGREVAGEVLDTRVHRGIVHYDP
TELVITARAGTPLRELLAALEAAGQRLPCEPPAFGDDATVGGMVAAGLSGPRRPWAGSVR
DFVLGTRLISGHGTVLRFGGEVMKNVAGYDLSRLLAGSFGCLGVITEVSLKVLPKPRHSL
SIRLELGSAEALETLAEWGRQPLPISAASHDGDCLHLRLEGGEGSVSAAHQRFGGEVIDD
RYWTALNEHRLAFFDEGLPLWRLSLPNHTGPLTLPGAQLIDWGGAQRWLKTETGTVQALA
HEVGGHATCYRQGASDTPFQPLAPALLRYHRQLKAQLDPLGLFNPGRMYPEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory