SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AO356_01100 FitnessBrowser__pseudo5_N2C3_1:AO356_01100 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 163:258/280 of Q007T0

query
sites
Q007T0

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W201 (≠ L40) binding

8gs8B Cryo-em structure of the human respiratory complex ii (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 129:224/239 of 8gs8B

query
sites
8gs8B

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N

binding fe3-s4 cluster: C162 (= C35), S164 (≠ T37), Y172 (≠ L45), P175 (= P48), C209 (= C75), H210 (≠ L76), T211 (≠ S77), I212 (≠ C78), M213 (≠ R79), N214 (= N80), C215 (= C81)
binding iron/sulfur cluster: C152 (= C25), I153 (≠ V26), L154 (≠ H27), C155 (= C28), A156 (≠ G29), C158 (= C31), A176 (vs. gap), C219 (= C85)
binding ubiquinone-1: P163 (= P36), W167 (≠ L40), H210 (≠ L76), I212 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 59, 60, 62, 64, 65, 66, 67, 79

4ytpB Crystal structure of porcine heart mitochondrial complex ii bound with n-[(4-tert-butylphenyl)methyl]-2-(trifluoromethyl)benzamide (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 4ytpB

query
sites
4ytpB

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binding N-(4-tert-butylbenzyl)-2-(trifluoromethyl)benzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 55, 56, 57, 58, 60, 62, 63, 64, 65, 75, 77

3sfeB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and thiabendazole (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 128:223/240 of 3sfeB

query
sites
3sfeB

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binding fe3-s4 cluster: C161 (= C35), S163 (≠ T37), Y171 (≠ L45), P174 (= P48), C208 (= C75), H209 (≠ L76), T210 (≠ S77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C156 (≠ F30), C157 (= C31), A175 (vs. gap), C218 (= C85), P219 (= P86), K220 (≠ S87), L222 (≠ V89)
binding 2-(1,3-thiazol-4-yl)-1h-benzimidazole: P162 (= P36), H209 (≠ L76), I211 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 59, 61, 62, 63, 64, 65, 66, 76, 78

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3sfdB

query
sites
3sfdB

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binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe3-s4 cluster: 227
binding fe2/s2 (inorganic) cluster: 55, 56, 57, 58, 60, 61, 62, 63, 64, 65, 75, 77
binding iron/sulfur cluster: 223

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aegB

query
sites
3aegB

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binding N-biphenyl-3-yl-2-iodobenzamide: P161 (= P36), S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe3-s4 cluster: 227
binding fe2/s2 (inorganic) cluster: 57, 58, 60, 61, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aeeB

query
sites
3aeeB

S

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binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: P161 (= P36), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 61, 62, 63, 64, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aedB

query
sites
3aedB

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binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 62, 63, 64, 65, 75, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aecB

query
sites
3aecB

S

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L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

D

N

binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 62, 63, 64, 65, 75, 77

3aebB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-phenoxy-phenyl)-2-trifluoromethyl-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aebB

query
sites
3aebB

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q
x
W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-(3-phenoxyphenyl)-2-(trifluoromethyl)benzamide: P161 (= P36), S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 62, 63, 65, 75, 77

3aeaB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-dimethylaminomethyl-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3aeaB

query
sites
3aeaB

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-{3-[(dimethylamino)methyl]phenyl}-2-(trifluoromethyl)benzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 62, 63, 65, 75, 77

3ae9B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-pentafluorophenyloxy-phenyl)-2-trifluoromethyl-benzamide (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae9B

query
sites
3ae9B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-[3-(pentafluorophenoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 56, 57, 58, 60, 61, 62, 63, 65, 77

3ae8B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-2-trifluoromethylbenzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae8B

query
sites
3ae8B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 75, 77

3ae7B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(3-isopropoxy-phenyl)-benzamide (see paper)
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae7B

query
sites
3ae7B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q
x
W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N

N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

D

N

binding 2-iodo-N-[3-(1-methylethoxy)phenyl]benzamide: P161 (= P36), S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe3-s4 cluster: 227
binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 75, 77

3ae6B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-phthalamicacid
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae6B

query
sites
3ae6B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-{[3-(1-methylethoxy)phenyl]carbamoyl}benzoic acid: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 55, 56, 57, 58, 60, 62, 63, 64, 65, 75, 77

3ae5B Crystal structure of porcine heart mitochondrial complex ii bound with 2-methyl-n-(3-isopropoxy-phenyl)-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae5B

query
sites
3ae5B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-
x
M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S

K

G

G

V

L

D

N

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-methyl-N-[3-(1-methylethoxy)phenyl]benzamide: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), M177 (vs. gap), C217 (= C85), P218 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 55, 56, 57, 58, 60, 61, 62, 63, 64, 65, 75, 77

3ae4B Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-methyl-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae4B

query
sites
3ae4B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), P173 (= P48), C207 (= C75), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-iodo-N-methylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 75, 77

3ae3B Crystal structure of porcine heart mitochondrial complex ii bound with 2-nitro-n-phenyl-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae3B

query
sites
3ae3B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding 2-nitro-N-phenylbenzamide: P161 (= P36), S162 (≠ T37), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 75, 77

3ae2B Crystal structure of porcine heart mitochondrial complex ii bound with 2-hydroxy-n-phenyl-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae2B

query
sites
3ae2B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87), L221 (≠ V89)
binding 2-hydroxy-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 61, 62, 63, 64, 65, 75, 77

3ae1B Crystal structure of porcine heart mitochondrial complex ii bound with n-phenyl-2-(trifluoromethyl)-benzamide
32% identity, 21% coverage: 6:90/406 of query aligns to 127:222/239 of 3ae1B

query
sites
3ae1B

S

S

E

Q

E

E

S

G

K

K

N

Q

-

Q

-

Y

L

L

P

Q

R

S

A

I

E

E

A

R

E

E

R

K

I

L

-

D

-

G

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F
x
C

C
|
C

N

S

A

T

T

S

C
|
C

P
|
P

T
x
S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

Q

R

P

L

A

A

T

K

A

L

S

Q

T

D

Q

P

L

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

S
x
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

T

G

T

T

C
|
C

P

P

S
x
K

G

G

V
x
L

D

N

binding fe3-s4 cluster: C160 (= C35), S162 (≠ T37), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (≠ S77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C155 (≠ F30), C156 (= C31), A174 (vs. gap), C217 (= C85), K219 (≠ S87), L221 (≠ V89)
binding N-phenyl-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)

Sites not aligning to the query:

binding fe3-s4 cluster: 227
binding fe2/s2 (inorganic) cluster: 57, 58, 60, 61, 62, 63, 64, 65, 75, 77

Query Sequence

>AO356_01100 FitnessBrowser__pseudo5_N2C3_1:AO356_01100
MQTRFSEESKNLPRAEEAERILRSCVHCGFCNATCPTYQLLGDELDGPRGRIYLIKQVLE
GQPATASTQLHLDRCLSCRNCETTCPSGVDYHNLLDIGRAVVDHAVPRPAGQRALRLGLR
SLAVSPERFKALLRLGAVFRPLLPTNLSAKLPHPAPSHSDRPPLRHGRRVLMLEGCVQPG
LSPNTNISAARVLDRLGISVIPCAQAGCCGALDYHLDAQATGLDRARQNIDAWWPHLEHG
AEAIVQTASGCGAFIKDYGHLLEHDPAYAAKAKQVSERALDLVQVLGNEPLEQICAARNQ
RIAVHCPCTLQHAQKLGGSIEALLTRLGFNLTEVPDGHLCCGSAGTYSLTQPALARQLRD
NRLNALESGQPEMIVTANVGCQNHLDGAGRTPVRHWIELVDQSLAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory