SitesBLAST

C28 (≠ S27) mutation to G: Slight decrease in activity; when associated with A-271.
Q56 (= Q55) mutation to C: Residual activity.
F59 (= F58) mutation to C: Loss of activity.
Q66 (≠ E65) mutation to C: Residual activity.
S69 (≠ L68) mutation to C: Residual activity.
C271 (≠ T283) mutation to A: Slight decrease in activity; when associated with G-28.
G349 (= G360) mutation to C: Loss of activity.
K355 (= K366) mutation K->C,G,Q,T: Loss of activity.; mutation to R: Residual activity.
G362 (≠ I373) mutation to C: Residual activity.
G363 (≠ P374) mutation to C: Residual activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8hpkA Crystal structure of the bacterial oxalate transporter oxlt in an oxalate-bound occluded form (see paper)
36% identity, 93% coverage: 12:412/432 of query aligns to 3:390/390 of 8hpkA

query
sites
8hpkA

V

L

G

G

G

N

R

R

W

W

M

F

Q

Y

L

L

L

V

A

L

G

A

V

V

I

L

C

L

M

M

S

C

M

M

I

I

A

S

N

G

L

V

Q
|
Q

Y
|
Y

G

S

W

W

T

T

L

L

F

Y

V

A

E

N

P

P

I

V

D

K

D

D

K

N

H

L

G

G

W

V

G

S

R

L

A

A

I

V

Q

Q

V

T

A

A

F

F

T

T

I

L

F

S

I

Q

V

V

T

I

E

Q

T

A

W

G

L

S

V

Q

P

P

V

G

E

G

G

G

W

Y

F

F

V

V

D

D

R

K

F

F

G

G

P

P

R

R

I

I

V

P

V

L

L

M

F

F

G

G

S

G

V

A

L

M

C

V

A

L

A

A

S

G

W

W

A

T

L

F

N

M

A

G

V

M

A

V

D

D

S

S

L

V

L

P

V

A

L

L

Y

Y

V

A

A

L

A

Y

A

T

I

L

G

A

G

G

I

A

G

G

A

V

G

G

A

I

V

V

Y
|
Y

G

G

T

I

C

A

V

M

G

N

N

T

A

A

L

N

K

R

W

W

F

F

P

P

D

D

R

K

R

R

G

G

L

L

A

A

A

S

G

G

I

F

T

T

A

A

G

G

F
x
Y

G

G

A

L

G

G

S

V

A

L

L

P

T

F

V

L

V

P

P

L

I

I

A

S

T

S

M

V

I

L

E

K

T

V

S

E

G

G

Y

V

E

G

A

A

T

A

F

F

M

M

A

Y

F

T

G

G

L

L

G

I

Q

M

G

G

L

I

V

L

V

I

F

I

A

L

L

I

A

A

W

F

L

V

L

I

V

R

A

F

P

P

P

-

S

-

A

-

P

-

L

-

A

-

R

-

G

-

G

G

F

Q

A

Q

T

I

P

V

A

T

Q

D

R

K

Q

D

Y

F

T

N

P

S

S

G

Q

E

M

M

V

L

R

R

T

T

P

P

V

Q

F

F

W

W

V

V

M

L

Y

W

A

T

M

A

F

F

V

F

M

S

V

V

A

N

G

F

G

G

L

L

M

L

A

L

T

V

A

A

Q

N

L

S

G

V

P

P

I

Y

A

G

Q

R

D

S

F

L

G

G

L

L

A

A

H

A

A

G

P

-

V

-

S

-

I

-

L

-

G

-

L

-

T

-

L

-

P

-

A

V

L

L

T

T

F

I

A

G

L

V

S

S

I

I

D

Q

R

N

V

L

L

F

N

N

G

G

L

G

T

C

R
|
R

P

P

F

F

F

W

G

G

W

F

V

V

S

S

D

D

H

K

I

I

G

G

R

R

E

Y

N

K

T

T

M

M

F

S

I

V

A

V

F

F

A

G

I

I

E

N

A

A

V

V

G

-

I

V

V

L

A

A

L

L

-

F

-

P

S

T

L

I

Y

A

G

A

R

L

D

G

P

D

V

V

A

A

F

F

V

I

L

A

L

M

T

L

G

A

L

I

V

A

F

F

A

T

W

W

G

G

E

G

I

S

Y
|
Y

S

A

L

L

F

F

P

P

A

S

C

T

C

N

A

S

D

D

T

I

F

F

G

G

S

T

K

A

F

Y

A

S

A

A

S

R

N

N

A

Y

G

G

L

F

Y

W

T

A

A

A

K
|
K

G

A

T

T

A

A

A

S

L

I

L

F

I

G

P

G

F

G

A

L

N

G

I

A

A

I

D

A

A

T

T

N

G

F

S

G

W

W

H

N

A

T

V

A

F

F

L

L

L

I

A

T

A

A

A

I

V

T

N

S

A

F

L

I

A

A

A

F

V

A

L

L

A

A

L

T

F

F

V

V

L

I

-

P

-

R

-

M

-

G

R

R

P

P

M

V

R

K

binding oxalate ion: Q24 (= Q33), Y25 (= Y34), Y114 (= Y123), Y140 (≠ F149), R257 (= R284), Y313 (= Y339), K340 (= K366)

6zguA Crystal structure of a mfs transporter with bound 3-(2-methylphenyl) propanoic acid at 2.41 angstroem resolution
31% identity, 94% coverage: 15:418/432 of query aligns to 4:398/404 of 6zguA

query
sites
6zguA

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

K

-

P

-

G

-

M

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

-

L

I

A

A

W

G

L

A

L

F

V

L

A

E

P

P

S

P

A

A

P

G

L

W

A

K

R

P

G

A

G

G

F

Y

A

-

T

T

P

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y

F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

A

L

E

E

binding 3-(2-methylphenyl)propanoic acid: F54 (≠ E65), Y113 (= Y123), L139 (≠ F149), R264 (= R284), F319 (≠ Y339), C346 (≠ K366)

6zgtA Crystal structure of a mfs transporter with bound 2-naphthoic acid at 2.39 angstroem resolution
31% identity, 94% coverage: 15:418/432 of query aligns to 4:398/404 of 6zgtA

query
sites
6zgtA

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I
x
C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

K

-

P

-

G

-

M

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

-

L

I

A

A

W

G

L

A

L

F

V

L

A

E

P

P

S

P

A

A

P

G

L

W

A

K

R

P

G

A

G

G

F

Y

A

-

T

T

P

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

A

L

E

E

binding naphthalene-2-carboxylic acid: C51 (≠ I62), F54 (≠ E65), Y113 (= Y123), L139 (≠ F149), R264 (= R284), F319 (≠ Y339), F343 (≠ Y363), C346 (≠ K366)

6zgsA Crystal structure of a mfs transporter with bound 3-phenylpropanoic acid at 2.39 angstroem resolution
31% identity, 94% coverage: 15:418/432 of query aligns to 4:398/404 of 6zgsA

query
sites
6zgsA

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

K

-

P

-

G

-

M

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

-

L

I

A

A

W

G

L

A

L

F

V

L

A

E

P

P

S

P

A

A

P

G

L

W

A

K

R

P

G

A

G

G

F

Y

A

-

T

T

P

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y

F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

A

L

E

E

binding hydrocinnamic acid: F54 (≠ E65), Y113 (= Y123), L139 (≠ F149), R264 (= R284), F319 (≠ Y339), C346 (≠ K366)

A0LNN5 L-lactate transporter; SfMCT from Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) (see paper)
30% identity, 95% coverage: 7:416/432 of query aligns to 5:412/412 of A0LNN5

query
sites
A0LNN5

Q

Q

S

T

A

T

L

M

P

P

V

-

G

-

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q
x
L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E

F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

K

-

P

-

G

-

M

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

-

L

I

A

A

W

G

L

A

L

F

V

L

A

E

P

P

S

P

A

A

P

G

L

W

A

K

R

P

G

A

G

G

F

Y

A

T

T

P

P

P

-

A

-

P

-

A

-

G

-

A

-

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q
x
H

L

L

G

A

P

G

I

F

A

G

Q
x
R

D
|
D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N
|
N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W
x
Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A
x
K

D
|
D

A

T

T

T

G

G

S

T

W
x
Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

L28 (≠ Q33) mutation to A: Loss of transport activity.
Y119 (= Y123) binding ; mutation Y->A,F: Loss of transport activity.
L145 (≠ F149) mutation to A: Strong decrease in transport activity.
H250 (≠ Q244) mutation to A: Strong decrease in transport activity.; mutation to F: Loss of transport activity.
R256 (≠ Q250) mutation to A: No change in transport activity.; mutation to D: Increases transport activity.
D257 (= D251) mutation to A: Loss of transport activity.
N276 (= N280) mutation to A: Loss of transport activity.
R280 (= R284) binding ; mutation to A: Abolishes L-lactate binding and L-lactate transport.
Y331 (≠ W335) mutation to A: Loss of transport activity.; mutation to F: No change in transport activity.
F335 (≠ Y339) mutation to A: Increases transport activity.
F359 (≠ Y363) mutation to A: Loss of transport activity.
C362 (≠ K366) mutation to A: Decrease in transport activity.
K377 (≠ A380) mutation K->A,D: No change in transport activity.
D378 (= D381) mutation to A: Loss of transport activity.
Y383 (≠ W386) mutation to A: Loss of transport activity.; mutation to F: Strong decrease in transport activity.

6zgrA Crystal structure of a mfs transporter with bound 1- hydroxynaphthalene-2-carboxylic acid at 2.67 angstroem resolution
31% identity, 94% coverage: 15:418/432 of query aligns to 4:393/399 of 6zgrA

query
sites
6zgrA

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

S

W

-

G

-

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

W

G

R

Y

Y

E

-

A

-

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

I

L

A

A

G

W

A

L

F

L

L

V

E

A

P

P

P

S

A

A

G

P

W

L

K

A

P

R

A

G

G

G

Y

F

T

A

A

T

A

P

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W
x
Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K

C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

A

L

E

E

binding 1-hydroxynaphthalene-2-carboxylic acid: F52 (≠ E65), Y111 (= Y123), L137 (≠ F149), R259 (= R284), Y310 (≠ W335), F314 (≠ Y339), F338 (≠ Y363)

6g9xA Crystal structure of a mfs transporter at 2.54 angstroem resolution (see paper)
31% identity, 94% coverage: 15:418/432 of query aligns to 4:390/396 of 6g9xA

query
sites
6g9xA

R

R

W

W

M

V

Q

P

L

L

A

L

G

G

V

L

I

L

C

G

M

S

S

T

M

T

I

C

A

G

N

M

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

S

G

-

W

-

G

-

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E

F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

I

T

E

S

W

G

R

Y

Y

E

-

A

-

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

L

A

I

L

A

A

G

W

A

L

F

L

L

V

E

A

P

P

P

S

A

A

G

P

W

L

K

A

P

R

A

G

G

G

-

F

-

A

A

T

A

P

P

P

K

A

V

Q

T

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W
x
Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y

F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

M

H

A

A

A

L

E

E

binding 2-sulfanylbenzoic acid: Y110 (= Y123), L136 (≠ F149), R256 (= R284), Y307 (≠ W335), F311 (≠ Y339), C338 (≠ K366)

6zguB Crystal structure of a mfs transporter with bound 3-(2-methylphenyl) propanoic acid at 2.41 angstroem resolution
31% identity, 88% coverage: 32:410/432 of query aligns to 15:364/364 of 6zguB

query
sites
6zguB

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

-

T

-

S

I

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

-

A

-

L

-

A

-

W

-

L

-

V

-

A

-

P

-

S

-

A

-

P

-

L

L

A

I

R

A

G

G

G

A

F

F

A

L

T

E

P

P

P

-

A

-

Q

-

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K

C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

binding 3-(2-methylphenyl)propanoic acid: F48 (≠ E65), Y107 (= Y123), L133 (≠ F149), R238 (= R284), F293 (≠ Y339), F317 (≠ Y363)

6zgtB Crystal structure of a mfs transporter with bound 2-naphthoic acid at 2.39 angstroem resolution
31% identity, 88% coverage: 32:410/432 of query aligns to 15:364/364 of 6zgtB

query
sites
6zgtB

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I
x
C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

-

T

-

S

I

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

-

A

-

L

-

A

-

W

-

L

-

V

-

A

-

P

-

S

-

A

-

P

-

L

L

A

I

R

A

G

G

G

A

F

F

A

L

T

E

P

P

P

-

A

-

Q

-

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

binding naphthalene-2-carboxylic acid: C45 (≠ I62), F48 (≠ E65), Y107 (= Y123), L133 (≠ F149), R238 (= R284), F293 (≠ Y339), F317 (≠ Y363), C320 (≠ K366)

6zgsB Crystal structure of a mfs transporter with bound 3-phenylpropanoic acid at 2.39 angstroem resolution
31% identity, 88% coverage: 32:410/432 of query aligns to 15:364/364 of 6zgsB

query
sites
6zgsB

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

G

W

W

G

S

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

-

T

-

S

I

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

-

A

-

L

-

A

-

W

-

L

-

V

-

A

-

P

-

S

-

A

-

P

-

L

L

A

I

R

A

G

G

G

A

F

F

A

L

T

E

P

P

P

-

A

-

Q

-

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W

Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K
x
C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

binding hydrocinnamic acid: F48 (≠ E65), Y107 (= Y123), L133 (≠ F149), R238 (= R284), F293 (≠ Y339), F317 (≠ Y363), C320 (≠ K366)

6g9xB Crystal structure of a mfs transporter at 2.54 angstroem resolution (see paper)
30% identity, 89% coverage: 32:414/432 of query aligns to 17:368/368 of 6g9xB

query
sites
6g9xB

L

L

Q
x
L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

S

W

-

G

-

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E

F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

-

T

-

S

I

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

-

A

-

L

-

A

-

W

-

L

-

V

-

A

-

P

-

S

-

A

-

P

-

L

L

A

I

R

A

G

G

G

A

F

F

A

L

T

E

P

P

P

-

A

-

Q

-

R

R

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W
x
Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y

F

T

T

A

A

K

C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

M

P

R

E

N

K

R

K

binding 2-sulfanylbenzoic acid: L18 (≠ Q33), Y107 (= Y123), L133 (≠ F149), R238 (= R284), Y289 (≠ W335), F293 (≠ Y339)

6zgrB Crystal structure of a mfs transporter with bound 1- hydroxynaphthalene-2-carboxylic acid at 2.67 angstroem resolution
30% identity, 88% coverage: 32:410/432 of query aligns to 17:363/364 of 6zgrB

query
sites
6zgrB

L

L

Q

L

Y

Y

G

A

W

W

T

S

L

V

F

F

V

I

E

K

P

P

I

L

D

N

D

A

K

E

H

F

G

S

W

-

G

-

R

R

A

A

A

E

I

I

Q

A

V

M

A

A

F

F

T

A

I

I

F

C

I

C

V

L

T

I

E
x
F

T

G

W

L

L

M

V

T

P

F

V

P

E

A

G

G

W

R

F

L

V

S

D

D

R

K

F

M

G

G

P

P

R

R

I

K

V

V

L

M

F

T

G

G

S

G

V

V

L

L

C

L

A

A

A

I

S

G

W

F

A

I

L

L

N

S

A

G

V

F

A

I

D

Q

S

S

L

K

L

Y

V

Q

L

L

Y

Y

V

I

A

T

-

Y

A

G

A

V

I

I

G

A

G

G

I

F

G

G

A

G

G

G

A

M

V

I

Y
|
Y

G

L

T

P

C

P

V

I

G

A

N

T

A

A

L

P

K

K

W

W

F

W

P

P

D

D

R

R

G

A

L

L

A

A

A

T

G

G

I

F

T

A

A

V

G

G

F
x
L

G

G

A

L

G

G

S

S

A

F

L

L

T

-

V

M

V

G

P

P

I

L

A

A

T

T

M

Y

I

I

E

-

T

-

S

I

G

G

Y

W

E

R

A

Y

T

V

F

F

M

W

A

Y

F

C

G

G

L

V

G

A

Q

M

G

G

L

I

V

M

V

A

F

-

A

-

L

-

A

-

W

-

L

-

V

-

A

-

P

-

S

-

A

-

P

-

L

L

A

I

R

A

G

G

G

A

F

F

A

L

T

E

P

P

P

-

A

-

Q

-

R

-

Q

D

Y

W

T

T

P

Y

S

E

Q

E

M

A

V

K

R

G

T

D

P

T

V

K

F

F

W

W

V

L

M

L

Y

Y

A

L

M

A

F

Y

V

F

M

C

V

G

A

S

G

F

G

A

G

G

L

L

M

M

A

V

T

I

A

G

Q

H

L

L

G

A

P

G

I

F

A

G

Q

R

D

D

F

-

G

-

L

-

A

-

H

-

A

-

P

-

V

-

S

-

I

-

L

A

G

G

L

L

T

T

L

A

P

M

A

A

L

A

T

A

F

G

A

A

L

V

S

S

I

S

D

L

R

A

V

F

L

S

N

N

G

A

L

A

T

T

R
|
R

P

I

F

L

F

S

G

G

W

W

V

F

S

V

D

D

H

K

I

I

G

G

R

I

E

R

N

V

T

Y

M

F

F

A

I

A

A

L

F

F

A

A

I

L

E

Q

A

T

V

A

G

A

I

M

V

I

A

A

L

I

S

F

L

Q

Y

L

G

G

R

G

D

S

P

V

V

V

A

G

F

L

V

S

L

I

L

V

T

A

G

I

L

V

V

I

F

G

F

W

A

N

W
x
Y

G

G

E

A

I

M

Y
x
F

S

T

L

L

F

F

P

P

A

A

C

T

C

C

A

L

D

Q

T

F

F

Y

G

G

S

P

K

T

F

A

A

Q

A

G

S

S

N

N

A

Y

G

G

L

L

Y
x
F

T

T

A

A

K

C

G

G

T

L

A

A

A

G

L

F

L

A

I

G

P

P

F

W

-

V

A

G

N

G

I

W

I

L

A

K

D

D

A

T

T

T

G

G

S

T

W

Y

H

Y

A

L

V

P

F

F

L

L

L

C

A

A

V

L

N

C

A

A

L

L

A

G

A

T

V

A

L

I

A

-

L

V

F

F

V

M

L

T

R

K

P

P

binding 1-hydroxynaphthalene-2-carboxylic acid: F48 (≠ E65), Y107 (= Y123), L133 (≠ F149), R237 (= R284), Y288 (≠ W335), F292 (≠ Y339), F316 (≠ Y363)

O60669 Monocarboxylate transporter 2; MCT 2; Solute carrier family 16 member 7 from Homo sapiens (Human) (see paper)
23% identity, 83% coverage: 70:429/432 of query aligns to 74:445/478 of O60669

query
sites
O60669

P

P

V

V

E

S

G

S

W

V

F

L

V

V

D

N

R

K

F

Y

G

G

P

S

R

R

I

P

V

V

L

I

F

A

G

G

S

G

V

L

L

L

C

C

A

C

A

L

S

G

W

M

A

V

L

L

N

A

A

S

V

F

A

S

D

S

S

S

L

V

V

Q

L

L

Y

Y

V

L

A

T

A

M

A

G

-

F

I

I

G

T

G

G

I

L

G

G

A

L

G

A

A

F

V

N

Y

L

G

Q

T

P

C

A

V

L

G

T

N

I

A

I

L

G

K

K

W

Y

F

F

P

Y

D

R

R

K

R
|
R

G

P

L

M

A

A

A
x
N

G

G

I

L

T

A

A

M

A

-

G

-

F

-

G

-

A

A

G

G

S

S

A

P

L

V

T

F

V

L

V

S

P

S

I

L

A

A

-

P

-

F

-

N

-

Q

T

Y

M

L

I

F

E

N

T

T

S

F

G

G

Y

W

E

K

A

G

T

S

F

F

M

L

A

I

F

-

G

-

L

L

G

G

Q

S

G

L

L

L

V

L

V

N

F

A

A

C

L

V

A

A

W

G

L

S

L

L

V

M

A

R

P

P

-

L

-

G

P

P

S

N

A

Q

P

T

L

T

A

S

R

K

G

S

G

K

F

N

A

K

T

T

P

G

P

K

A

T

Q

E

R

D

Q

D

Y

S

T

S

P

P

S

K

Q

K

M

I

V

K

R

T

T

K

P

K

V

S

F

T

W

W

V

E

-

K

-

V

-

N

M

K

Y

Y

A

L

M

D

F

F

V

S

M

L

V

F

A

K

G

H

G

R

G

G

L

F

M

L

A

I

T

Y

A

L

Q

S

L

-

G

G

P

N

I

V

A

I

Q

M

D

F

F

L

G

G

L

F

A

-

H
x
F

A

A

P

P

V

I

S

I

I

F

L

L

-

A

-

P

-

Y

-

A

-

K

-

D

-

Q

G

G

L

I

T

D

L

E

P

Y

A

S

L

A

T

A

F

F

A

L

L

L

S

S

I

V

D

M

R

A

V

F

L

V

N
x
D

G

M

L

F

T

A

R
|
R

P

P

F

S

F

V

G

G

W

L

V

I

-

A

-
x
N

S

S

D

K

H

Y

I

I

G

R

R

P

E

R

N

I

T

Q

M

Y

F

F

I

F

A

S

F

F

A

A

I

I

E

M

A

F

V

N

G

G

I

V

V

C

A

H

L

L

S

L

L

C

-

P

Y

L

G

A

R

Q

D

D

P

Y

V

T

A

S

F

L

V

V

L

L

L

Y

T

A

G

V

L

F

V

F

F

G

F

L

A

G

W
x
F

G

G

E

S

I

V

Y
x
S

S

S

L

V

F

L

P

F

A
x
E

C

T

C

L

A

M

D

D

T

L

F

V

G

G

S

A

K

P

F

R

A

F

A

S

S

S

N

A

A

V

G

G

L

L

L

V

Y

T

T

I

A

V

K

E

G

C

T

G

A

P

A

V

L

L

-

G

I

P

P

P

F

L

A

A

N

G

I

K

I

L

A

V

D

D

A

L

T

T

G

G

S

E

W

Y

H

K

A

Y

V

M

F

Y

L

M

L

S

A

C

A

G

A

A

V

I

N

-

A

V

L

V

A

A

V

S

L

V

A

W

L

L

F

L

V

I

L

G

R

N

P

A

M

I

R

N

N

Y

R

R

M

L

A

L

A

A

E

K

S

E

L

R

D

K

A

E

R

E

S

N

G

A

R

R

S

Q

V

K

T
|
T

R143 (= R138) mutation to A: Reduces pyruvate transmembrane transporter activity. Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization. Dominant negative mutant.
N147 (≠ A142) mutation to A: Reduces pyruvate transmembrane transporter activity. Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
F262 (≠ H256) mutation to A: No effect on protein abundance. Does not affect cell surface localization.
D293 (≠ N280) May be protonated during monocarboxylate transport; mutation to N: Reduced proton-dependent active symport, but not pyruvate transport.
R297 (= R284) mutation R->L,D: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
N305 (vs. gap) mutation to A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
F351 (≠ W335) mutation to A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
S355 (≠ Y339) mutation S->G,A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
E360 (≠ A344) mutation to A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
T445 (= T429) to S: in dbSNP:rs3763980

Sites not aligning to the query:

18 W→A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization. Dominant negative mutant.
20 W→A: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization. Dominant negative mutant.
34 Y→F: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
38 K→D: Reduces pyruvate transmembrane transporter activity. No effect on protein abundance. Does not affect cell surface localization.
70 Y→A: No effect on protein abundance. Does not affect cell surface localization.

Q6ZSM3 Monocarboxylate transporter 12; MCT 12; Creatine transporter 2; CRT2; Solute carrier family 16 member 12 from Homo sapiens (Human) (see 3 papers)
21% identity, 78% coverage: 77:413/432 of query aligns to 110:468/516 of Q6ZSM3

query
sites
Q6ZSM3

D

N

R

H

F

L

G

S

P

C

R

Q

I

V

V

G

V

I

L

M

F

L

G

G

S

G

V

L

L

L

C

A

A

S

A

T

S

G

W

L

A

I

L

L

N

S

A

S

V

F

A

A

D

T

S

S

L

L

L

K

V

H

L

L

Y

Y

V

L

A

T

-

L

A

G

A

V

I

L

G

T

G

G

I

L

G

G

A

F

G

A

A

L

V

C

Y

Y

G

S

T

P

C

A

V

I

G

A

N

M

A

V

L

G

K

K

W

Y

F

F

P

S

D

R

R

R

R

K

G

A

L

L

A

A

A

Y

G

G

I

I

T

A

A

M

A

S

G

G

F

S

G

G

A

I

G

G

S

T

A

F

L

I

T

L

V

A

V

P

P

V

I

V

A

Q

T

L

M

L

I

I

E

E

T

Q

S

F

G

S

Y

W

E

R

A

G

T

A

F

L

M

L

A

I

F

L

G

G

L

-

G

-

Q

-

G

-

L

-

V

-

V

G

F

F

A

V

L

L

A

N

W

L

L

C

L

V

V

C

A

G

P

A

P

L

S

M

A

R

P

P

L

I

A

T

R

L

G

K

G

E

F

D

A

H

T

T

P

T

P

P

A

E

Q

Q

R

N

Q

H

Y

V

T

C

P

R

S

T

Q
|
Q

-
x
K

-
x
E

-
x
D

-
x
I

-
x
K

-
x
R

-
x
V

-
x
S

-
x
P

-
x
Y

-
x
S

-
x
L

-
x
T

-
x
K

-
x
E

-
x
W

-
x
A

-
x
Q

-
x
T

-
x
C

-
x
L

-
x
C

M
x
L

V
x
Q

R
x
Q

T
x
E

P
x
Y

V
x
S

F
|
F

W
x
L

V
x
L

M
|
M

Y
x
S

A
x
D

M
x
F

F
x
V

V
|
V

M
x
L

V
x
A

A
x
V

G
x
S

G
x
V

G
x
L

L
x
F

M
|
M

A
|
A

T
x
Y

-
x
G

-
x
C

-
x
S

-
x
P

-
x
L

-
x
F

A
x
V

Q
x
Y

L
|
L

G
x
V

P
|
P

I
x
Y

A
|
A

Q
x
L

D
x
S

F
x
V

G
|
G

L
x
V

A
x
S

H
|
H

A
x
Q

P
x
Q

V
x
A

S
x
A

I

-

L

-

G

-

L

-

T

-

L

-

P

-

A

-

L

-

T

-

F
|
F

A
x
L

L
x
M

S
|
S

I
|
I

D
x
L

R
x
G

V
|
V

L
x
I

N
x
D

G
x
I

L
x
I

T
x
G

R
x
N

P
x
I

F
x
T

F
|
F

G
|
G

W
|
W

V
x
L

S
x
T

D
|
D

H
x
R

I
x
R

G
x
C

R
x
L

E
x
K

N
|
N

T
x
Y

M
x
Q

F
x
Y

I
x
V

A
x
C

-
x
Y

-
x
L

F
|
F

A
|
A

I
x
V

E
x
G

A
x
M

V
x
D

G
|
G

I
x
L

V
x
C

A
x
Y

L
|
L

S
x
C

L
|
L

Y
x
P

G
x
M

R
x
L

D
x
Q

P
x
S

V
x
L

A
x
P

F
x
L

V
x
L

L
x
V

L
x
P

T
x
F

G
x
S

L
x
C

V
x
T

F
|
F

-
x
G

F
x
Y

A
x
F

W
x
D

G
|
G

E
x
A

I
x
Y

Y
x
V

S
x
T

L
|
L

F
x
I

P
|
P

A
x
V

C
x
V

C
x
T

A
x
T

D
x
E

T
x
I

F
x
V

G
|
G

S
x
T

K
x
T

F
x
S

A
x
L

A
x
S

S
|
S

N
x
A

A
x
L

G
|
G

L
x
V

Y
|
Y

T
x
F

A
x
L

K
x
H

G
x
A

T
x
V

A
x
P

A
x
Y

L
|
L

L
x
V

I
x
S

-
x
P

P
|
P

F
x
I

A
|
A

N
x
G

I
x
R

I
x
L

A
x
V

D
|
D

A
x
T

T
|
T

G
|
G

S
|
S

W
x
Y

H
x
T

A
|
A

V
x
A

F
|
F

L
|
L

A
x
C

A
x
G

A
x
F

V
x
S

N
x
M

A
x
I

L
x
F

A
x
S

V
|
V

L
|
L

A
x
L

L
x
G

F
|
F

-
x
A

-
x
R

V
x
L

L
x
I

R
x
K

P
x
R

M
|
M

R
|
R

N
x
K

D329 (≠ N280) mutation to A: Decreases creatine efflux activity. Loss of localization to the plasma membrane.
D360 (≠ V309) mutation to A: Does not affect creatine efflux activity. Does not affect plasma membrane localization.
D387 (≠ W335) mutation to A: Does not affect creatine efflux activity.
G437 (= G384) to S: found in a patient with age-related cataract; uncertain significance; decreases creatine transport; dbSNP:rs759863805

Sites not aligning to the query:

67 R→A: Abolishes creatine efflux activity. Does not affect plasma membrane localization.
95 D→A: Decreases in the creatine efflux activity. Does not affect plasma membrane localization.
245:516 natural variant: Missing (in CTRCT47; loss of localization to the plasma membrane; retained in the endoplasmic reticulum where it undergoes degradation by the proteasome; has no dominant effect on wild-type protein expression and localization)

Query Sequence

>AZOBR_RS07945 FitnessBrowser__azobra:AZOBR_RS07945
MRQTGAQSALPVGGRWMQLLAGVICMSMIANLQYGWTLFVEPIDDKHGWGRAAIQVAFTI
FIVTETWLVPVEGWFVDRFGPRIVVLFGSVLCAASWALNAVADSLLVLYVAAAIGGIGAG
AVYGTCVGNALKWFPDRRGLAAGITAAGFGAGSALTVVPIATMIETSGYEATFMAFGLGQ
GLVVFALAWLLVAPPSAPLARGGFATPPAQRQYTPSQMVRTPVFWVMYAMFVMVAGGGLM
ATAQLGPIAQDFGLAHAPVSILGLTLPALTFALSIDRVLNGLTRPFFGWVSDHIGRENTM
FIAFAIEAVGIVALSLYGRDPVAFVLLTGLVFFAWGEIYSLFPACCADTFGSKFAASNAG
LLYTAKGTAALLIPFANIIADATGSWHAVFLLAAAVNALAAVLALFVLRPMRNRMAAESL
DARSGRSVTATP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory