SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS08900 FitnessBrowser__azobra:AZOBR_RS08900 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
54% identity, 98% coverage: 2:171/174 of query aligns to 4:173/185 of 4jxrB

query
sites
4jxrB

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binding acetyl coenzyme *a: A29 (= A27), D86 (= D83), S87 (= S84), I88 (= I85), Y89 (= Y86), L90 (= L87), P92 (= P89), R95 (≠ M92), G96 (= G93), G100 (= G97), H127 (≠ N125), S129 (= S127), S130 (= S128), H134 (= H132)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
48% identity, 97% coverage: 3:171/174 of query aligns to 6:175/181 of 5dwnA

query
sites
5dwnA

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binding acetyl coenzyme *a: S87 (= S84), I88 (= I85), Y89 (= Y86), I90 (≠ L87), K95 (≠ M92), G96 (= G93), G98 (= G95), G100 (= G97), K101 (≠ R98), G131 (≠ S127), S132 (= S128), K134 (≠ G130), L135 (= L131), H136 (= H132)

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
41% identity, 93% coverage: 3:163/174 of query aligns to 6:165/165 of 4jwpA

query
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4jwpA

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binding acetyl coenzyme *a: A30 (= A27), V88 (≠ I85), Y89 (= Y86), V90 (≠ L87), R95 (≠ M92), G96 (= G93), G98 (= G95), G100 (= G97), R101 (= R98), G122 (≠ V119), A129 (≠ S127), S130 (= S128), A132 (≠ G130)
binding calcium ion: E110 (≠ R107), E113 (= E110)

3dr8A Structure of ynca, a putative acetyltransferase from salmonella typhimurium with its cofactor acetyl-coa
39% identity, 99% coverage: 1:173/174 of query aligns to 3:172/173 of 3dr8A

query
sites
3dr8A

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binding acetyl coenzyme *a: A29 (= A27), H85 (≠ D83), S86 (= S84), V87 (≠ I85), Y88 (= Y86), V89 (≠ L87), Q94 (≠ M92), G95 (= G93), G97 (= G95), G99 (= G97), R100 (= R98), A127 (= A126), A128 (≠ S127), R131 (≠ G130), H133 (= H132), S135 (= S134)

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
40% identity, 97% coverage: 2:170/174 of query aligns to 3:171/171 of 2j8mA

query
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2j8mA

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binding azide ion: W73 (≠ Y71), R74 (= R72)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
40% identity, 97% coverage: 2:170/174 of query aligns to 4:172/172 of A6VCX3

query
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A6VCX3

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RPF 75:77 (≠ RLR 72:74) binding
EHS 85:87 (≠ EDS 82:84) binding

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 99% coverage: 1:173/174 of query aligns to 1:170/171 of Q8ZPD3

query
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Q8ZPD3

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S

S

I

I

G

R

L

L

H

H

R

H

S
|
S

L

L

G

G

F

F

E

T

P

V

A

T

G

A

L

Q

L

M

K

P

A

Q

V

V

G

G

F

V

K

K

F

F

G

G

R

R

W

W

V

L

D

D

S

L

V

T

L

F

M

M

Q

Q

R

L

P

Q

L

L

G

-

N

-

G

D

E

E

A

H

S

A

P

A

P

P

D

D

G

A

E82 (= E82) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (≠ IYL 85:87) binding
GKGLGR 93:98 (≠ GRGAGR 93:98) binding
N124 (= N125) binding
S133 (= S134) binding

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
40% identity, 97% coverage: 2:170/174 of query aligns to 2:170/170 of 2j8rA

query
sites
2j8rA

N

S

I

I

R

R

S

D

S

A

R

G

L

V

D

A

D

D

L

L

E

P

R

G

I

I

Q

L

A

A

I

I

Y

Y

A

N

H

D

H

A

V

V

L

G

T

N

G

T

A

T

A
|
A

S
x
I

F
x
W

E

N

E

E

V

T

P

P

P

V

S

D

L

L

E

A

E

N

L

R

A

Q

R

A

R
x
W

R

F

D

D

D

A

V

R

L

A

A

R

R

Q

G

G

M

Y

P

P

Y

I

I

L

V

V

A

A

-

S

E

D

M

A

G

A

G

G

R

E

V

V

I

L

G

G

Y
|
Y

A

A

Y

S

T

Y

G

G

P

D

Y

W

R
|
R

L

P

R
x
F

T

E

A

G

Y
x
F

R

R

Y

G

S

T

V

V

E
|
E

D

H

S
|
S

I

V

Y

Y

L

V

D

R

P

D

D

D

C

Q

M

R

G

G

R

K

G

G

A

L

G

G

R

V

A

Q

L

L

S

Q

T

A

V

L

I

I

D

E

R

R

C

A

E

R

E

A

A

Q

G

G

C

L

R

H

Q

V

M

M

I

V

A

A

V

A

I

I

G

-

D

E

S

S

A

G

N

N

A

A

S

A

S

S

I

I

G

G

L

L

H

H

R

R

S

R

L

L

G

G

F

F

E

E

P

I

A

S

G

G

L

Q

L

M

K

P

A

Q

V

V

G

G

F

Q

K

K

F

F

G

G

R

R

W

W

V

L

D

D

S

L

V

T

L

F

M

M

Q

Q

R

L

P

N

L

L

G

D

N

P

G

T

E

R

A

S

S

A

P

P

binding azide ion: W42 (≠ R42), Y66 (= Y65), S85 (= S84)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (= A27), I28 (≠ S28), W29 (≠ F29), R73 (= R72), F75 (≠ R74), F78 (≠ Y77), E83 (= E82)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
40% identity, 89% coverage: 1:155/174 of query aligns to 2:154/179 of 5wphA

query
sites
5wphA

M

I

N

D

I

I

R

R

S

V

S

A

R

R

L

P

D

E

D

D

L

A

E

E

R

E

I

I

Q

Q

A

I

I

I

Y

Y

A

A

H

P

H

I

V

V

L

L

T

N

G

T

A

A

A

I

S
|
S

F
|
F

E

E

V

A

P

V

P

P

S

S

L

V

E

E

E

Q

L

M

A

-

R

R

R

E

R

R

D

I

D

S

V

T

L

T

A

L

R

Q

G

T

M

Y

P

P

Y

Y

I

L

V

V

A

A

E

V

M

R

G

E

G

G

R

R

V

V

I

V

G

G

Y

Y

A

A

Y

Y

T

A

G

S

P

Q

Y

H

R
|
R

L

A

R
|
R

T

A

A

A

Y
|
Y

R

R

Y

W

S

A

V

V

E

D

D

V

S

T

I

V

Y

Y

L

V

D

A

P

E

D

G

C

Q

M

R

G

R

R

S

G

G

A

I

G

A

R

R

A

Q

L

L

L

Y

S

D

T

V

V

L

I

L

D

P

R

V

C

L

E

K

E

R

A

L

G

G

C

Y

R

R

Q

S

M

A

I

Y

A

A

V

G

I

I

G

A

D

-

S

L

A

P

N

N

A

E

S

G

S

S

I

V

G

G

L

L

H

H

R

E

S

R

L

L

G

G

F

F

E

Q

P

H

A

I

G

G

L

T

L

F

K

P

A

Q

V

V

G

G

F

F

K

K

F

L

G

D

R

A

W

W

V

H

D

D

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: S29 (= S28), F30 (= F29), R72 (= R72), R74 (= R74), Y77 (= Y77)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
40% identity, 89% coverage: 1:155/174 of query aligns to 2:154/176 of 6m7gA

query
sites
6m7gA

M

I

N

D

I

I

R

R

S

V

S

A

R

R

L

P

D

E

D

D

L

A

E

E

R

E

I

I

Q

Q

A

I

I

I

Y

Y

A

A

H

P

H

I

V

V

L

L

T

N

G

T

A

A

A

I

S

S

F

F

E

E

V

A

P

V

P

P

S

S

L

V

E

E

E

Q

L

M

A

-

R

R

R

E

R

R

D

I

D

S

V

T

L

T

A

L

R

Q

G

T

M

Y

P

P

Y

Y

I

L

V

V

A

A

E

V

M

R

G

E

G

G

R

R

V

V

I

V

G

G

Y

Y

A

A

Y

Y

T

A

G

S

P

Q

Y

H

R

R

L

A

R
|
R

T

A

A

A

Y
|
Y

R

R

Y

W

S

A

V

V

E

D

D

V

S

T

I

V

Y

Y

L

V

D

A

P

E

D

G

C

Q

M

R

G

R

R

S

G

G

A

I

G

A

R

R

A

Q

L

L

L

Y

S

D

T

V

V

L

I

L

D

P

R

V

C

L

E

K

E

R

A

L

G

G

C

Y

R

R

Q

S

M

A

I

Y

A

A

V

G

I

I

G

A

D

-

S

L

A

P

N

N

A

E

S

G

S

S

I

V

G

G

L

L

H

H

R

E

S

R

L

L

G

G

F

F

E

Q

P

H

A

I

G

G

L

T

L

F

K

P

A

Q

V

V

G

G

F

F

K

K

F

L

G

D

R

A

W

W

V

H

D

D

binding phosphinothricin: R74 (= R74), Y77 (= Y77)

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
34% identity, 91% coverage: 2:160/174 of query aligns to 3:159/175 of 5t7eD

query
sites
5t7eD

N

D

I

I

R

R

S

R

S

A

R

T

L

E

D

A

D

D

L

M

E

P

R

A

I

V

Q

C

A

T

I

I

Y

V

A

N

H

H

H
x
Y

V

I

L

E

T

T

G

S

A

T

A
x
V

S

N

F
|
F

E

R

E

T

V

E

P

P

P

Q

S

E

L

P

E

Q

E

E

L

W

A

T

R

-

D

D

V

L

-

V

-

R

L

L

A

R

R

E

G

R

M

Y

P

P

Y

W

I

L

V

V

A

A

E

E

M

V

G

D

G

G

R

E

V

V

I

A

G

G

Y

I

A

A

Y

Y

T

A

G

G

P

P

Y

W

R
x
K

L

A

R
|
R

T

N

A

A

Y
|
Y

R

D

Y

W

S

T

V

A

E

E

D

S

S
x
T

I
x
V

Y
|
Y

L
x
V

D

S

P

P

D

R

C

H

M
x
Q

G
x
R

R
x
T

G
|
G

A

L

G
|
G

R
x
S

A

T

L

L

L

Y

S

T

T

H

V

L

I

L

D

K

R

S

C

L

E

E

E

A

A

Q

G

G

C

F

R

K

Q

S

M

V

I

V

A

A

V
|
V

I

I

G
|
G

D

-

S

L

A

P

N

N

A

D

S
x
P

S
|
S

I

V

G

R

L
x
M

H

H

R

E

S

A

L

L

G

G

F

Y

E

A

P

P

A

R

G

G

L

M

L

L

K

R

A

A

V

A

G

G

F

F

K

K

F

H

G

G

R

N

W

W

V

H

D

D

S

V

V

G

L

F

M

W

Q

Q

binding coenzyme a: Y22 (≠ H21), V28 (≠ A27), V85 (≠ I85), Y86 (= Y86), V87 (≠ L87), Q92 (≠ M92), R93 (≠ G93), T94 (≠ R94), G95 (= G95), G97 (= G97), S98 (≠ R98), P126 (≠ S127), S127 (= S128), M130 (≠ L131)
binding phosphinothricin: F30 (= F29), K72 (≠ R72), R74 (= R74), Y77 (= Y77), T84 (≠ S84), Y86 (= Y86), V119 (= V119), G121 (= G121)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
34% identity, 91% coverage: 2:160/174 of query aligns to 2:158/173 of 5t7dA

query
sites
5t7dA

N

D

I

I

R

R

S

R

S

A

R

T

L

E

D

A

D

D

L

M

E

P

R

A

I

V

Q

C

A

T

I

I

Y

V

A

N

H

H

H
x
Y

V

I

L

E

T

T

G

S

A

T

A
x
V

S

N

F

F

E

R

E

T

V

E

P

P

P

Q

S

E

L

P

E

Q

E

E

L

W

A

T

R

-

D

D

V

L

-

V

-

R

L

L

A

R

R

E

G

R

M

Y

P

P

Y

W

I

L

V

V

A

A

E

E

M

V

G

D

G

G

R

E

V

V

I

A

G

G

Y

I

A

A

Y

Y

T

A

G

G

P

P

Y

W

R

K

L

A

R

R

T

N

A

A

Y

Y

R

D

Y

W

S

T

V

A

E

E

D

S

S
x
T

I
x
V

Y
|
Y

L
x
V

D

S

P

P

D

R

C

H

M
x
Q

G
x
R

R

T

G
|
G

A

L

G
|
G

R
x
S

A

T

L

L

L

Y

S

T

T

H

V

L

I

L

D

K

R

S

C

L

E

E

E

A

A

Q

G

G

C

F

R

K

Q

S

M

V

I

V

A

A

V
|
V

I

I

G

G

D

-

S

L

A

P

N

N

A

D

S
x
P

S
|
S

I

V

G
x
R

L
x
M

H

H

R

E

S

A

L

L

G

G

F

Y

E

A

P

P

A

R

G

G

L

M

L

L

K

R

A

A

V

A

G

G

F

F

K

K

F

H

G

G

R

N

W

W

V

H

D

D

S

V

V

G

L

F

M

W

Q

Q

binding acetyl coenzyme *a: Y21 (≠ H21), V27 (≠ A27), T83 (≠ S84), V84 (≠ I85), Y85 (= Y86), V86 (≠ L87), Q91 (≠ M92), R92 (≠ G93), G94 (= G95), G96 (= G97), S97 (≠ R98), V118 (= V119), P125 (≠ S127), S126 (= S128), R128 (≠ G130), M129 (≠ L131)

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
34% identity, 93% coverage: 3:163/174 of query aligns to 5:164/165 of 4mbuA

query
sites
4mbuA

I

I

R

R

S

C

S

A

R

K

L

K

D

E

D

D

L

L

E

N

R

A

I

I

Q

L

A

A

I

I

Y

Y

A

N

H

D

H

A

V

I

L

I

T

N

G

T

A

T

A

A

S

V

F

Y

E

T

E

Y

V

K

P

P

P

Q

S

T

L

I

E

D

E

E

L

R

A

I

R

A

R

W

R

F

D

E

D

T

V

K

L

Q

A

R

R

N

G

H

M

E

P

P

Y

I

I

F

V

V

A

F

E

E

M

E

G

N

G

G

R

S

V

V

I

L

G

G

Y

F

A

A

Y

T

T

F

G

G

P

S

Y

F

R

R

L

P

R

W

T

P

A

A

Y

Y

R

Q

Y

Y

S

T

V

I

E

E

D

H

S

S

I

I

Y

Y

L

V

D

D

P

A

D

S

C

A

M

R

G

G

R

K

G

G

A

I

G

A

R

S

A

Q

L

L

S

Q

T

R

V

L

I

I

D

V

R

E

C

A

E

K

E

A

A

K

G

G

C

Y

R

R

Q

T

M

L

I

V

A

A

V

G

I

I

G

-

D

D

S

A

A

S

N

N

A

E

S

A

S

S

I

I

G

K

L

L

H

H

R
x
Q

S

K

L

F

G

N

F

F

E
x
K

P
x
H

A

A

G

G

L

T

L

L

K

T

A

N

V

V

G

G

F

Y

K

K

F

F

G

D

R

Y

W

W

V

L

D

D

S

L

V

A

L

F

M

Y

Q

E

R

L

P

D

L

L

binding phosphate ion: Q134 (≠ R133), K139 (≠ E138), H140 (≠ P139)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
35% identity, 82% coverage: 22:163/174 of query aligns to 30:171/180 of 2jlmF

query
sites
2jlmF

V

I

L

I

T

N

G

S

A
x
T

A
|
A

S

L

F

Y

E

D

E

Y

V

K

P

P

P

R

S

S

L

K

E

E

E

S

L

M

A

A

R

A

R

W

R

F

D

A

D

T

V

K

L

R

A

Q

R

N

G

N

M

F

P

P

Y

I

I

I

-

G

-

A

V

V

A

N

E

E

M

V

G

G

G

-

R

Q

V

L

I

L

G

G

Y

F

A

A

Y

S

T

W

G

G

P

S

Y

F

R

R

L

A

R

F

T

P

A

A

Y

Y

R

K

Y

Y

S

T

V

V

E

E

D

H

S

S

I

V

Y
|
Y

L
x
I

D

H

P

K

D

D

C

Y

M

R

G

G

R

L

G

G

A

L

G

S

R

K

A

H

L

L

L

M

S

N

T

E

V

L

I

I

D

K

R

R

C

A

E

V

E

E

A

S

G

E

C

V

R

H

Q

V

M

M

I

V

A

G

V

C

I

I

G

-

D
|
D

S

A

A

T

N
|
N

A

V

S

A

S

S

I

I

G

Q

L

L

H

H

R

Q

S

K

L

L

G

G

F

F

E

I

P

H

A

S

G

G

L

T

L

I

K

Q

A

Q

V

A

G

G

F

F

K

K

F

F

G

G

R

R

W

W

V

L

D

D

S

A

V

A

L

F

M

Y

Q

Q

R

L

P

T

L

L

binding azide ion: T34 (≠ A26), A35 (= A27), Y95 (= Y86), I96 (≠ L87), D130 (= D122), N133 (= N125)

P0A944 [Ribosomal protein bS18]-alanine N-acetyltransferase; KAT; Peptidyl-lysine N-acetyltransferase; EC 2.3.1.266; EC 2.3.1.- from Escherichia coli (strain K12) (see paper)
41% identity, 34% coverage: 84:142/174 of query aligns to 68:125/148 of P0A944

query
sites
P0A944

S

N

I

I

Y

A

L

V

D

D

P

P

D

D

C

Y

M

Q

G

R

R

Q

G

G

A

L

G

G

R

R

A

A

L

L

S

E

T

H

V

L

I

I

D

D

R

E

C

L

E

E

E

K

A

R

G

G

C

V

R

A

Q

T

M

L

I

W

A

L

V

E

I

V

G

R

D

A

S

S

A

-

N

N

A

A

S

A

I

I

G

A

L

L

H
x
Y

R

E

S

S

L

L

G

G

F

F

E

N

P

E

A

A

G

T

L

I

Y115 (≠ H132) mutation to A: Loss of activity.

Query Sequence

>AZOBR_RS08900 FitnessBrowser__azobra:AZOBR_RS08900
MNIRSSRLDDLERIQAIYAHHVLTGAASFEEVPPSLEELARRRDDVLARGMPYIVAEMGG
RVIGYAYTGPYRLRTAYRYSVEDSIYLDPDCMGRGAGRALLSTVIDRCEEAGCRQMIAVI
GDSANASSIGLHRSLGFEPAGLLKAVGFKFGRWVDSVLMQRPLGNGEASPPDGH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory