SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS11025 FitnessBrowser__azobra:AZOBR_RS11025 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33197 Isocitrate dehydrogenase [NADP]; ICDH; IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
44% identity, 98% coverage: 7:479/481 of query aligns to 22:490/496 of P33197

query
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P33197

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L88 (≠ I73) binding NADP(+)
T90 (= T75) binding NADP(+)
N193 (= N177) binding NADP(+)
Q229 (≠ R213) binding NADP(+)
K232 (≠ D216) binding NADP(+)
E277 (= E261) binding NADP(+)
G281 (= G265) binding NADP(+)
S282 (= S266) binding NADP(+)
A283 (= A267) binding NADP(+)
K285 (≠ M269) binding NADP(+)
Y286 (≠ I270) binding NADP(+)
N293 (= N277) binding NADP(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2d1cA Crystal structure of tt0538 protein from thermus thermophilus hb8
44% identity, 98% coverage: 7:479/481 of query aligns to 21:489/495 of 2d1cA

query
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2d1cA

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active site: Y143 (= Y128), K190 (= K175), D223 (= D208), D247 (= D232), D251 (= D236)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P86 (= P72), L87 (≠ I73), E88 (≠ T74), T89 (= T75), N99 (= N85), I221 (= I206), N224 (≠ I209), Q228 (≠ R213), L260 (≠ V245), G261 (= G246), H279 (= H264), G280 (= G265), S281 (= S266), A282 (= A267), K284 (≠ M269), Y285 (≠ I270), I291 (≠ A276), N292 (= N277), D333 (= D317)

6lkyA Crystal structure of isocitrate dehydrogenase from methylococcus capsulatus
48% identity, 70% coverage: 7:343/481 of query aligns to 4:339/339 of 6lkyA

query
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G

G

I

I

A

A

N
|
N

P

P

S

T

G

A

L

M

L

I

M

M

A

A

A

G

V

A

M

M

L

L

V

E

H

H

I

I

G

G

Q

E

G

P

D

D

V

A

A

A

A

R

R

R

I

I

H

E

N

R

A

A

W

V

L

R

K

E

T

V

I

I

E

E

D

D

G

G

V

R

H

S

T

V

V

T

D

P

I

D

F

L

N

A

R

K

G

D

V

-

S

-

R

-

Q

S

R

P

V

C

G

G

T

T

H

A

A

Q

F

M

A

A

D

E

A

A

V

I

V

V

E

E

R

R

L

V

G

R

Q

Q

active site: Y123 (= Y128), K174 (= K175), D207 (= D208), D231 (= D232)
binding nicotinamide-adenine-dinucleotide: P68 (= P72), L69 (≠ I73), T71 (= T75), N81 (= N85), H263 (= H264), G264 (= G265), S265 (= S266), A266 (= A267), D268 (≠ M269), I269 (= I270), N276 (= N277)

6m3sB Dimeric isocitrate dehydrogenase from xanthomonas campestris pv. Campestris 8004
47% identity, 70% coverage: 4:341/481 of query aligns to 5:338/338 of 6m3sB

query
sites
6m3sB

T

T

T

Q

P

T

I

I

T

T

V

V

A

I

R

R

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

M

D

D

A

A

V

T

L

L

H

F

V

V

M

L

E

D

A

A

A

L

G

Q

A

A

R

G

L

L

K

T

V

Y

E

E

E

Y

V

A

P

D

A

A

G

G

E

-

Q

L

V

V

Y

A

R

L

R

E

G

K

H

H

L

G

G

D

G

L

L

L

D

P

A

E

A

S

G

T

W

L

G

A

S

S

I

I

R

T

R

K

T

N

R

K

V

V

F

A

L

L

K

K

G

S

P
|
P

I
x
L

T

T

T
|
T

P

P

Q

V

G

G

Y

E

G

G

N

F

K

S

S
|
S

L

I

N
|
N

V

V

T

A

A

M

R
|
R

T

R

T

K

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R
|
R

P

P

C

A

V

K

S

S

H

F

H

-

P

P

Y

N

V

T

R

K

T

S

R

R

H

F

P

A

R

D

-

G

M

V

D

D

V

L

V

I

I

T

I

V

R
|
R

E

E

N

N

E

T

E

E

D

G

L

A

Y
|
Y

-

L

-

S

A

E

G

G

I

Q

E

E

H

V

R

S

Q

A

T

D

D

G

D

E

V

V

I

A

Q

V

S

S

V

G

K

A

L

R

I

V

S

T

R

R

P

K

G

G

S

S

E

E

R

R

I

I

V

V

R

R

Y

Y

A

A

F

F

D

D

Y

L

A

A

R

R

A

A

N

T

H

G

R

R

R

K

K

K

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N

N

V

I

M

I

K

K

M

S

T

T

D

S

G

G

L

L

F

F

L

L

K

K

I

V

F

A

N

R

E

D

I

V

A

A

A

T

E

Q

Y

Y

P

P

E

E

I

I

K

E

A

F

D

Q

H

E

L

M

I

I

V

V

D
|
D

I

N

G

T

A

C

A

M

R

Q

L

L

A

V

D

M

Q

R

P

P

E

E

R

Q

F

F

D

D

V

I

I

I

V

V

T

T

L

T

N

N

L

L

Y

F

G

G

D
|
D

I

I

V

I

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

V

G

G

S

G

V

L

G

G

L

L

A

A

G

P

S

G

A

A

N

N

I

I

G

G

D

V

A

D

C

A

A

A

M

I

F

F

E

E

A

A

I

V

H
|
H

G
|
G

S
|
S

A
|
A

P

P

M
x
D

I
|
I

A

A

G

G

Q

Q

G

G

I

K

A

A

N
|
N

P

P

S

C

G

A

L

L

M

L

A

G

A

A

V

A

M

Q

M

M

L

L

V

D

H

H

I

I

G

G

Q

Q

G

P

D

Q

V

N

A

A

A

E

R

R

I

L

H

R

N

E

A

A

W

I

L

V

K

A

T

T

I

L

E

E

-

A

-

K

D

D

G

S

V

L

H

-

T

T

V

P

D

D

I

L

F

G

N

G

R

T

G

G

V

-

S

-

R

-

Q

-

R

-

V

-

G

N

T

T

H

M

A

G

F

F

A

A

D

K

A

A

V

I

V

A

E

S

R

R

L

L

active site: Y128 (= Y128), K177 (= K175), D210 (= D208), D234 (= D232)
binding isocitrate calcium complex: T75 (= T75), S83 (= S83), N85 (= N85), R89 (= R89), R99 (= R99), R121 (= R121), Y128 (= Y128), D234 (= D232), D238 (= D236)
binding nicotinamide-adenine-dinucleotide: P72 (= P72), L73 (≠ I73), T75 (= T75), N85 (= N85), H266 (= H264), G267 (= G265), S268 (= S266), A269 (= A267), D271 (≠ M269), I272 (= I270), N279 (= N277)

6kdyA Crystal structure of the alpha bata heterodimer of human idh3 in complex with NAD. (see paper)
45% identity, 64% coverage: 7:312/481 of query aligns to 5:309/335 of 6kdyA

query
sites
6kdyA

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

R

P

N

A

V

G

-

E

T

Q

A

V

I

Y

Q

R

G

R

P

G

G

H

G

L

K

G

W

G

M

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P
|
P

I
x
L

T

K

T
|
T

P

P

Q

I

G

A

Y

A

G

G

N

H

K

P

S

S

L

M

N
|
N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D

D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H
|
H

G
|
G

S
x
T

A
|
A

P

P

M
x
D

I
|
I

A

A

G

G

Q

K

G

D

I

M

A

A

N
|
N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

active site: Y124 (= Y128), K171 (= K175), D204 (= D208), D228 (= D232)
binding nicotinamide-adenine-dinucleotide: P69 (= P72), L70 (≠ I73), T72 (= T75), N82 (= N85), H261 (= H264), G262 (= G265), T263 (≠ S266), A264 (= A267), D266 (≠ M269), I267 (= I270), N274 (= N277)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 315

6kdeA Crystal structure of the alpha beta heterodimer of human idh3 in complex with ca(2+) (see paper)
45% identity, 64% coverage: 7:312/481 of query aligns to 5:309/336 of 6kdeA

query
sites
6kdeA

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

R

P

N

A

V

G

-

E

T

Q

A

V

I

Y

Q

R

G

R

P

G

G

H

G

L

K

G

W

G

M

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

I

G

A

Y

A

G

G

N

H

K

P

S

S

L

M

N

N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H

H

G

G

S

T

A

A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N

N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

active site: Y124 (= Y128), K171 (= K175), D204 (= D208), D228 (= D232)
binding calcium ion: D228 (= D232), D232 (= D236)

P50213 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; EC 1.1.1.41 from Homo sapiens (Human) (see 5 papers)
45% identity, 64% coverage: 7:312/481 of query aligns to 34:338/366 of P50213

query
sites
P50213

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

R

P

N

A

V

G

-

E

T

Q

A

V

I

Y

Q

R

G

R

P

G

G

H

G

L

K

G

W

G

M

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

I

G

A

Y

A

G

G

N

H

K

P

S

S

L

M

N

N

V

L

T

L

A

L

R
|
R

T

K

T

T

L

F

G

D

L

L

F

Y

A
|
A

N

N

V

V

R
|
R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R
|
R

E

E

N

N

E

T

E

E

D

G

L
x
E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K
|
K

L

L

I

I

S

T

R

E

P

G

G
x
A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N
|
N

V

I

M
|
M

K

R

M

M

T

S

D
|
D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L
x
M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y
|
Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H

H

G

G

S

T

A

A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N

N

P
|
P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M
|
M

M

M

L

L

V
x
R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

R115 (= R89) binding substrate
A122 (= A96) to T: in RP90; uncertain significance; dbSNP:rs756333430
R125 (= R99) binding substrate
R146 (= R121) binding substrate
E152 (≠ L127) mutation to A: No significant effect on the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
Y153 (= Y128) Critical for catalysis; mutation to F: Complete loss of activity of the heterotetramer, heterodimer composed of IDH3A and IDH3B subunits and the heterodimer composed of IDH3A and IDH3G subunits with no effect on their oligomeric states.
K169 (= K144) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
A175 (≠ G150) to V: in RP90; uncertain significance; dbSNP:rs765473830
K200 (= K175) Critical for catalysis; mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate.
N202 (= N177) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate.
M204 (= M179) to I: in RP90; uncertain significance
D208 (= D183) mutation to A: Complete loss of the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
D233 (= D208) binding Mg(2+)
M239 (≠ L214) to T: in RP90; uncertain significance; dbSNP:rs2074707744
Y255 (= Y230) mutation to A: Significantly impairs the activation of the heterodimer composed of IDH3A and IDH3G subunits by citrate and ADP.
D257 (= D232) binding Mg(2+)
D261 (= D236) binding Mg(2+)
P304 (= P278) to H: in RP90; uncertain significance; dbSNP:rs756712426
M313 (= M287) to T: in RP90; uncertain significance; dbSNP:rs149862950
R316 (≠ V290) to C: in RP90; uncertain significance; dbSNP:rs770798851

Q9D6R2 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; EC 1.1.1.41 from Mus musculus (Mouse) (see paper)
44% identity, 64% coverage: 7:312/481 of query aligns to 34:338/366 of Q9D6R2

query
sites
Q9D6R2

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

S

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

R

P

N

A

V

G

-

E

T

Q

A

V

I

Y

Q

R

G

R

P

G

G

H

G

L

K

G

W

G

M

L

I

D

P

A

P

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

I

G

A

Y

A

G

G

N

H

K

P

S

S

L

M

N

N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y

Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

E

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K

K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

N

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H
x
E

L

M

I

Y

V

L

D

D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D

D

I

I

V

L

S

S

D

D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H

H

G

G

S

T

A

A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N

N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

K

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

E229 (≠ H204) mutation to K: Homozygous mutant mice exhibit retinal degeneration.

5yvtA Crystal structure of the alpha gamma heterodimer of human idh3 in complex with mg(2+) and nadh (see paper)
45% identity, 64% coverage: 7:312/481 of query aligns to 4:306/332 of 5yvtA

query
sites
5yvtA

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

R

P

N

A

V

G

-

E

T

Q

A

V

I

Y

Q

R

G

R

P

G

G

H

G

L

K

G

W

G

M

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I
x
L

T

K

T
|
T

P

P

Q

-

G

-

Y

I

G

A

N

A

K

P

S

S

L

M

N
|
N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N
|
N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E
|
E

A

S

I
x
V

H
|
H

G
|
G

S

T

A

A

P

P

M

D

I
|
I

A

A

G

G

Q

K

G

D

I

M

A

A

N
|
N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

active site: Y121 (= Y128), K168 (= K175), D201 (= D208), D225 (= D232), D229 (= D236)
binding magnesium ion: D225 (= D232), D229 (= D236)
binding 1,4-dihydronicotinamide adenine dinucleotide: L69 (≠ I73), T71 (= T75), N79 (= N85), N170 (= N177), D201 (= D208), E255 (= E261), V257 (≠ I263), H258 (= H264), G259 (= G265), I264 (= I270), N271 (= N277)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 312

6l59A Crystal structure of the alpha gamma heterodimer of human idh3 in complex with cit, mg and atp binding at allosteric site and mg, atp binding at active site. (see paper)
45% identity, 64% coverage: 7:312/481 of query aligns to 4:297/325 of 6l59A

query
sites
6l59A

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I
|
I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

-

R

-

N

V

V

P

T

A

A

G

I

E

Q

Q

W

V

M

Y

-

R

-

G

-

H

-

L

-

G

-

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

-

G

-

Y

-

G

-

N

-

K

-

S

S

L

M

N

N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H
|
H

G
|
G

S
x
T

A
|
A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N
|
N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

active site: Y112 (= Y128), K159 (= K175), D192 (= D208), D216 (= D232)
binding adenosine-5'-triphosphate: I12 (= I15), H249 (= H264), G250 (= G265), T251 (≠ S266), A252 (= A267), N262 (= N277)
binding magnesium ion: D216 (= D232), D220 (= D236)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 303

5greA Crystal structure of the alpha gamma heterodimer of human idh3 in complex with mg(2+), citrate and adp (see paper)
45% identity, 64% coverage: 7:312/481 of query aligns to 4:298/325 of 5greA

query
sites
5greA

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

-

R

-

N

V

V

P

T

A

A

G

I

E

Q

Q

W

V

M

Y

-

R

-

G

-

H

-

L

-

G

-

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

-

G

-

Y

-

G

-

N

-

K

P

S

S

L

M

N

N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K
|
K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I

Y

V

L

D
|
D

I

T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H

H

G

G

S

T

A

A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N

N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

active site: Y113 (= Y128), K160 (= K175), D193 (= D208), D217 (= D232), D221 (= D236)
binding magnesium ion: D217 (= D232), D221 (= D236)

8grdA Crystal structure of a constitutively active mutant of the alpha beta heterodimer of human idh3 in complex with adp and mg (see paper)
44% identity, 64% coverage: 7:312/481 of query aligns to 4:299/325 of 8grdA

query
sites
8grdA

I

V

T

T

V

L

A

I

R

P

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

A

A

A

V

V

L

M

H

K

V

I

M

F

E

D

A

A

G

K

A

A

R

P

L

I

K

Q

V

W

E

E

V

-

P

-

A

-

G

R

E

N

Q

V

V

T

Y

A

R

I

R

Q

G

G

H

W

L

M

G

-

L

I

D

P

A

S

A

E

G

A

W

K

G

E

S

S

I

M

R

D

R

K

T

N

R

K

V

M

F

G

L

L

K

K

G

G

P

P

I

L

T

K

T

T

P

P

Q

-

G

-

Y

-

G

-

N

-

K

P

S

S

L

M

N

N

V

L

T

L

A

L

R

R

T

K

T

T

L

F

G

D

L

L

F

Y

A

A

N

N

V

V

R

R

P

P

C

C

V

V

S

S

H

I

H

E

P

G

Y

Y

V

-

R

K

T

T

R

P

H

Y

P

T

R

D

M

V

D

N

V

I

V

V

I

T

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y

Y

A

S

G

G

I

I

E

E

H

H

R

V

Q

I

T

V

D

D

D

G

V

V

I

V

Q

A

S

S

V

I

K

K

L

L

I

I

S

T

R

E

P

G

G

A

S

S

E

K

R

R

I

I

V

A

R

E

Y

F

A

A

F

F

D

E

Y

Y

A

A

R

R

A

N

N

N

H

H

R

R

R

S

K

N

V

V

T

T

A

A

F

V

V

H

K

K

D

A

N

N

V

I

M

M

K

R

M

M

T

S

D

D

G

G

L

L

F

F

L

L

K

Q

I

K

F

C

N

R

E

E

I

V

A

A

A

E

E

S

Y

C

P

K

E

D

I

I

K

K

A

F

D

N

H

E

L

M

I
x
Y

V

L

D

D

I
x
T

G

V

A

C

A

L

R

N

L

M

A

V

D

Q

Q

D

P

P

E

S

R

Q

F

F

D

D

V

V

I

L

V

V

T

M

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

C

A

A

Q

G

I

L

T

I

G

G

S

G

V

L

G

G

L

V

A

T

G

P

S

S

A

G

N

N

I

I

G

G

-

A

D

N

A

G

C

V

A

A

M

I

F

F

E

E

A

S

I

V

H

H

G

G

S

T

A

A

P

P

M

D

I

I

A

A

G

G

Q

K

G

D

I

M

A

A

N

N

P

P

S

T

G

A

L

L

M

L

A

S

A

A

V

V

M

M

L

L

V

R

H

H

I

M

G

G

Q

L

G

F

D

D

V

H

A

A

R

R

I

I

H

E

N

A

A

A

W

C

L

F

K

A

T

T

I

I

E

K

D

D

G

G

binding adenosine-5'-diphosphate: Y192 (≠ I206), T195 (≠ I209)
binding magnesium ion: D218 (= D232), D222 (= D236)

3blwF Yeast isocitrate dehydrogenase with citrate and amp bound in the regulatory subunits (see paper)
42% identity, 70% coverage: 7:341/481 of query aligns to 21:347/347 of 3blwF

query
sites
3blwF

I

V

T

S

V

F

A

I

R

E

G

G

D

D

G

G

I

I

G

G

P

P

E

E

I

I

T

S

D

K

A

S

V

V

L

K

H

K

V

I

M

F

E

S

A

A

G

N

A

V

R

P

L

I

K

E

V

W

E

E

E

S

V

C

P

D

A

V

G

S

E

-

Q

P

V

I

Y

F

R

V

R

N

G

G

H

-

L

L

G

T

L

I

D

P

A

D

A

P

G

A

W

V

G

Q

S

S

I

I

R

T

R

K

T

N

R

L

V

V

F

A

L

L

K

K

G

G

P

P

I

L

T

A

T

T

P

P

Q

H

G

-

Y

-

G

-

N

-

K

R

S

S

L

L

N

N

V

L

T

T

A

L

R

R

T

K

T

T

L

F

G

G

L

L

F

F

A

A

N

N

V

V

R

R

P

P

C

A

V

K

S

S

H

I

H

E

P

G

Y

F

V

-

R

K

T

T

R

T

H

Y

P

E

R

N

M

V

D

D

V

L

V

V

I

L

I

I

R

R

E

E

N

N

E

T

E

E

D

G

L

E

Y
|
Y

A

S

G

G

I

I

E

E

H

H

R

I

Q

V

T

C

D

P

D

G

V

V

I

V

Q

Q

S

S

V

I

K

K

L

L

I

I

S

T

R

R

P

D

G

A

S

S

E

E

R

R

I

V

V

I

R

R

Y

Y

A

A

F

F

D

E

Y

Y

A

A

R

R

A

A

N

I

H

G

R

R

R

P

K

R

V

V

T

I

A

V

F

V

V

H

K
|
K

D

S

N
x
T

V

I

M

Q

K

R

M

L

T

A

D

D

G

G

L

L

F

F

L

V

K

N

I

V

F

A

N

K

E

E

I

L

A

S

A

K

E

E

Y

Y

P

P

E

D

I

L

K

T

A

L

D

E

H

T

L

E

I

L

V

I

D
|
D

I

N

G

S

A

V

A

L

R

K

L

V

A

V

D

T

Q

N

P

P

E

S

R

A

F

Y

D

T

-

D

-

A

V

V

I

S

V

V

T

C

L

P

N

N

L

L

Y

Y

G

G

D
|
D

I

I

V

L

S

S

D
|
D

I

L

A

N

A

S

Q

G

I

L

T

S

-

A

G

G

S

S

V

L

G

G

L

L

A

T

G

P

S

S

A

A

N

N

I

I

G

G

D

H

A

K

C

I

A

S

M

I

F

F

E

E

A

A

I

V

H

H

G

G

S

S

A

A

P

P

M

D

I

I

A

A

G

G

Q

Q

G

D

I

K

A

A

N

N

P

P

S

T

G

A

L

L

M

L

A

S

V

V

M

M

L

L

V

N

H

H

I

M

G

G

Q

L

G

T

D

N

V

H

A

A

A

D

R

Q

I

I

H

Q

N

N

A

A

W

V

L

L

K

S

T

T

I

I

E

A

D

S

G

G

V

P

H

E

T

-

V

-

D

-

I

-

F

-

N

N

R

R

-

T

G

G

V

D

S

L

R

A

Q

G

R

T

V

A

G

T

T

T

H

S

A

S

F

F

A

T

D

E

A

A

V

V

V

I

E

K

R

R

L

L

active site: Y135 (= Y128), K182 (= K175), D215 (= D208), D241 (= D232), D245 (= D236)
binding citrate anion: K182 (= K175), T184 (≠ N177)

P51553 Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma from Homo sapiens (Human) (see 2 papers)
40% identity, 67% coverage: 7:328/481 of query aligns to 57:376/393 of P51553

query
sites
P51553

I

V

T

T

V

M

A

I

R

P