SitesBLAST

C98 (= C110) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (= C112) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (= C140) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (= H142) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (≠ W152) mutation to Q: No change in activity or in zinc content.
H131 (≠ F154) mutation to Q: No change in activity or in zinc content.
H132 (≠ N155) mutation to Q: No change in activity or in zinc content.
C138 (≠ F159) mutation to S: No change in activity or in zinc content.
S239 (= S273) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
38% identity, 80% coverage: 18:281/329 of query aligns to 6:258/485 of Q8DLI5

query
sites
Q8DLI5

R
|
R

F

L

A

A

P

P

S

S

P

P

T

T

G

G

P

N

L

L

H

H

A

I

G

G

S

T

L

A

V

R

A

T

A

A

L

V

A

F

S

N

W

W

L

L

D

Y

A

A

R

R

A

H

H

R

G

G

Q

K

W

F

L

I

V

L

R

R

I

I

E

E

D

D

V

T

D

D

T

R

P

E

R

R

C

S

V

R

P

P

G

E

A

Y

D

T

Q

E

F

N

I

I

L

L

Q

E

Q

G

L

L

A

Q

T

W

C

L

G

G

L

L

V

T

P

W

D

D

A

E

P

G

P

P

E

Y

W

F

Q

Q

S

S

A

D

R

R

S

L

A

D

H

L

Y

Y

Q

R

R

Q

A

A

L

I

D

Q

Q

T

L

L

V

L

A

D

Q

K

G

G

H

L

A

A

Y

Y

P

Y

C

C

A

Y

C

C

S

T

R

P

K

E

D

E

V

L

E

E

D

A

-

L

-

R

-

A

A

E

Q

Q

A

K

A

A

L

K

G

G

H

Q

A

A

-

P

-

R

-

Y

R

D

E

N

R

R

H

H

A

R

A

H

L

L

P

T

Y

P

P

E

G

E

T

Q

C

A

R

A

H

F

G

E

L

A

R

A

G

G

R

R

T

T

G

P

R

-

S

V

W

I

R

R

F

F

N

K

A

I

T

E

D

D

F

D

K

R

Q

Q

K

-

Q

-

A

-

L

-

P

-

D

-

G

-

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

-

L

I

H

E

W

W

H

Q

D

D

R

L

R

V

L

R

G

G

L

R

Q

V

Q

S

Q

W

D

Q

V

G

A

A

R

D

S

L

V

G

G

G

D

D

F

M

V

V

L

I

H

A

R

R

A

A

D

A

-

P

-

R

-

G

-

E

-

I

G

G

L

Y

W

P

A

L

Y
|
Y

Q

N

L

L

A

V

V

V

D

D

A

I

A

A

Q

M

G

G

I

I

T

T

D

D

V

V

V

I

R

R

G

G

E

E

D

D

L

H

A

I

D

G

N

N

T

T

P

P

R

K

Q

Q

I

I

L

L

Q

Y

Q

E

A

A

L

L

G

G

V

A

P

T

T

P

P

P

R

N

Y

F

L

A

H

H

T

T

P

P

L

L

V

I

C

L

G

N

A

S

N

T

G

G

E

Q

K

K

L

L

S

S

K

K

Q

R

N

D

G

G

A

V

R

T

A

S

L

I

R6 (= R18) binding L-glutamate
Y192 (= Y215) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
38% identity, 80% coverage: 18:281/329 of query aligns to 5:257/484 of 2cfoA

query
sites
2cfoA

R
|
R

F

L

A

A

P

P

S
|
S

P

P

T

T

G

G

P

N

L

L

H

H

A

I

G

G

S

T

L

A

V

R

A

T

A

A

L

V

A

F

S

N

W

W

L

L

D

Y

A

A

R

R

A

H

H

R

G

G

Q

K

W

F

L

I

V

L

R

R

I

I

E

E

D

D

V

T

D

D

T

R

P

E

R

R

C

S

V

R

P

P

G

E

A

Y

D

T

Q

E

F

N

I

I

L

L

Q

E

Q

G

L

L

A

Q

T

W

C

L

G

G

L

L

V

T

P

W

D

D

A

E

P

G

P

P

E

Y

W

F

Q

Q

S

S

A

D

R

R

S

L

A

D

H

L

Y

Y

Q

R

R

Q

A

A

L

I

D

Q

Q

T

L

L

V

L

A

D

Q

K

G

G

H

L

A

A

Y

Y

P

Y

C

C

A

Y

C

C

S

T

R

P

K

E

D

E

V

L

E

E

D

A

-

L

-

R

-

A

A

E

Q

Q

A

K

A

A

L

K

G

G

H

Q

A

A

-

P

-

R

-

Y

R

D

E

N

R

R

H

H

A

R

A

H

L

L

P

T

Y

P

P

E

G

E

T

Q

C

A

R

A

H

F

G

E

L

A

R

A

G

G

R

R

T

T

G

P

R

-

S

V

W

I

R

R

F

F

N

K

A

I

T

E

D

D

F

D

K

R

Q

Q

K

-

Q

-

A

-

L

-

P

-

D

-

G

-

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

-

L

I

H

E

W

W

H

Q

D

D

R

L

R

V

L

R

G

G

L

R

Q

V

Q

S

Q

W

D

Q

V

G

A

A

R

D

S

L

V

G

G

G

D

D

F

M

V

V

L

I

H

A

R

R

A

A

D

A

-

P

-

R

-

G

-

E

-

I

G

G

L

Y

W

P

A

L

Y
|
Y

Q

N

L

L

A

V

V

V

D

D

A

I

A

A

Q

M

G

G

I

I

T

T

D

D

V

V

V

I

R
|
R

G

G

E

E

D

D

L
x
H

A

I

D

G

N

N

T

T

P

P

R

K

Q

Q

I

I

L

L

Q

Y

Q

E

A

A

L

L

G

G

V

A

P

T

T

P

P

P

R

N

Y

F

L

A

H

H

T

T

P

P

L
|
L

V

I

C

L

G

N

A

S

N

T

G

G

E

Q

K

K

L

L

S

S

K
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K

Q

R

N

D

G

G

A

V

R

T

A

S

L

I

active site: S9 (= S22), L239 (= L263), K250 (= K274)
binding glutamic acid: R5 (= R18), Y191 (= Y215), R209 (= R233), H213 (≠ L237)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
31% identity, 81% coverage: 18:282/329 of query aligns to 106:343/564 of 3al0C

query
sites
3al0C

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

H

L

L

H

H

A

V

G
|
G

S

G

L

A

V

R

A
x
T

A

A

L

L

A

F

S

N

W

W

L

M

D

F

A

A

R

R

A

K

H

E

G

G

Q

K

W

F

L

I

V

L

R

R

I

I

E
|
E

D

D

V
x
T

D
|
D

T

T

P

E

R
|
R

C

S

V

S

P

R

G

E

A

Y

D

E

Q

Q

F

Q

I

I

L

L

Q

E

Q

S

L

L

A

R

T

W

C

C

G

G

L

L

V

D

P

W

D

D

A

E

P

G

P

P

E

D

W

I

-

G

-

D

-

F

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

E

H

I

Y

Y

Q

R

R

E

A

Y

L

A

D

E

Q

K

L

L

V

V

A

E

Q

D

G

K

H

R

A

A

Y

Y

P

Y

C

V

A

V

C
x
Y

S

D

R

K

K

E

D

D

V

-

E

-

D

-

A

-

Q

-

A

-

L

-

G

-

H
x
P

A

S

R

K

E

E

R

L

H

F

A

T

L

Y

P

E

Y

Y

P

P

G

H

T

-

C

-

R

-

H

-

G

-

L

-

R

-

G

-

R

-

T

-

G

-

R

-

S

-

W

-

R

-

F

-

N

-

A

-

T

-

D

E

F

Y

K

K

Q

E

K

K

Q

-

A

-

L

-

P

-

D

-

G

G

Y

H

A

P

S

V

S

T

A

I

I

K

N

F

S

K

I

V

A

L

N

P

N

G

T

K

L

T

H

S

W

F

H

E

D

D

R

L

L
x
K

G

G

L

Y

Q
x
M

Q

E

Q

F

D

D

V

N

A

S

R

-

S

T

V

L

G

E

D

D

F

F

V
x
I

L

I

H

M

R
x
K

A
x
S

D

N

G

G

L

F

W

P

A

T

Y
|
Y

Q

N

L

F

A

A

V

V

D

D

A

H

A

L

Q

M

G

R

I

I

T

S

D

H

V

V

V

F

R
|
R

G
|
G

E

E

D
|
D

L
x
H

A
x
L

D
x
S

N
|
N

T

T

P

P

R
x
K

Q

Q

I

L

L

M

L

I

Q

Y

Q

E

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

V

Y

F

L

M

H

H

T

I

P

P

L
|
L

V
x
I

C

L

G

G

A

S

N

D

G
x
R

E

T

K
x
P

L
|
L

S

S

K
|
K

Q

R

N

H

G

G

A

A

R

T

A

S

L

V

D

E

active site: S110 (= S22), K335 (= K274)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R18), A108 (= A20), P109 (= P21), G118 (= G30), T122 (≠ A34), E142 (= E54), Y276 (= Y215), R294 (= R233), G295 (= G234), D297 (= D236), H298 (≠ L237), L324 (= L263), I325 (≠ V264), L333 (= L272)
binding : T144 (≠ V56), D145 (= D57), R148 (= R60), Y208 (≠ C112), P213 (≠ H126), K252 (≠ L190), M255 (≠ Q193), I266 (≠ V205), K269 (≠ R208), S270 (≠ A209), Y276 (= Y215), D297 (= D236), H298 (≠ L237), L299 (≠ A238), S300 (≠ D239), N301 (= N240), K304 (≠ R243), R330 (≠ G269), P332 (≠ K271)

Sites not aligning to the query:

binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
34% identity, 80% coverage: 18:281/329 of query aligns to 6:232/380 of 4g6zA

query
sites
4g6zA

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

F

L

I

H

H

A

L

G

G

S

N

L

I

V

R

A

S

A

A

L

L

A

Y

S

P

W

W

L

A

D

F

A

A

R

R

A

K

H

M

G

K

G

G

Q

T

W

F

L

V

V

L

R

R

I

I

E
|
E

D

D

V

T

D

D

T

V

P

E

R

R

C

S

V

S

P

Q

G

E

A

A

D

V

Q

D

F

A

I

I

L

L

Q

E

Q

G

L

M

A

A

T

W

C

L

G

G

L

L

V

D

P

Y

D

D

A

E

P

G

P

P

E

Y

W

Y

Q

Q

S

M

A

Q

R

R

S

M

A

D

H

R

Y

Y

Q

R

R

E

A

V

L

L

D

A

Q

Q

L

M

V

Q

A

E

Q

K

G

G

H

L

A

V

Y

Y

P

P

C

C

A

-

C

-

S

-

R

-

K

-

D

-

V

-

E

-

D

-

A

-

Q

-

A

-

L

-

G

-

H

Y

A

M

R

R

E

Y

R

D

H

G

A

T

A

W

L

R

P

P

Y

E

P

P

G

G

T

K

C

V

R

-

H

-

G

-

L

-

R

-

G

-

R

-

T

-

G

-

R

-

S

-

W

-

R

-

F

-

N

-

A

-

T

-

D

-

F

-

K

-

Q

-

K

-

Q

L

A

P

L

E

P

P

D

A

G

G

Y

V

A

A

S

P

S

V

A

L

-

R

I

F

N

R

S

N

I

P

A

L

N

T

N

G

T

T

L

V

H

A

W

W

H

D

D

D

R

A

R

V

L

K

G

G

L

-

Q

R

Q

V

Q

E

D

I

V

S

A

N

R

E

S

E

V

L

G

D

D

D

F

L

V

V

L

V

H

A

R

R

A

P

D

D

G

G

L

T

W

P

A

M

Y

Y

Q

N

L

F

A

C

V

V

D

D

A

L

A

D

Q

M

G

G

I

I

T

T

D

H

V

V

V

I

R
|
R

G

G

E

D

D

D

L
x
H

A

V

D

N

N

N

T

T

P

P

R

R

Q

Q

I

I

L

N

L

I

Q

L

Q

R

A

A

L

L

G

G

V

G

P

E

T

V

P

P

R

V

Y

Y

L

A

H

H

T

L

P

P

L
x
T

V

V

C

L

G

N

A

E

N

Q

G

G

E

E

K

K

L

M

S

S

K
|
K

Q

R

N

H

G

G

A

A

R

M

A

S

L

V

active site: S10 (= S22), T214 (≠ L263), K225 (= K274)
binding glutamic acid: R6 (= R18), A8 (= A20), S10 (= S22), E42 (= E54), R184 (= R233), H188 (≠ L237)

6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid
30% identity, 80% coverage: 18:279/329 of query aligns to 6:266/502 of 6brlA

query
sites
6brlA

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

P

L

L

H

H

A

L

G

G

S

G

L

V

V

R

A

T

A

A

L

L

A

Y

S

D

W

Y

L

L

D

F

A

A

R

K

A

H

H

N

G

G

Q

D

W

F

L

I

V

L

R

R

I

I

E
|
E

D

D

V

T

D

D

T

T

P

Q

R

R

C

Y

V

V

P

P

G

G

A

S

D

E

Q

E

F

Y

I

I

L

M

Q

E

Q

A

L

L

A

E

T

W

C

I

G

G

L

M

V

V

P

P

D

D

A

E

P

S

P

P

E

K

W

H

-

G

-

P

-

Y

-

A

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

R

A

D

H

I

Y

Y

Q

D

R

R

A

Y

L

T

D

E

Q

Q

L

I

V

L

A

K

Q

T

G

D

H

Y

A

A

Y

Y

P

L

C

A

A

F

C

D

S

T

R

P

K

E

D

E

V

L

E

D

D

Q

A

I

Q

R

A

A

A

E

L

F

G

-

H

-

A

-

R

E

E

A

R

R

H

G

A

D

A

V

L

F

P

A

Y

Y

P

N

G

Y

T

E

C

T

R

R

H

N

G

R

L

L

R

R

G

N

R

S

T

I

G

S

-

L

-

P

-

E

-

V

R

K

S

K

W

L

R

L

F

E

N

E

A

K

T

T

D

P

F

Y

K

V

Q

I

K

R

Q

F

A

K

L

M

P

P

P

L

D

D

G

R

Y

I

A

I

S

N

S

-

A

-

I

L

N

N

S

D

I

I

A

I

N

R

N

G

T

K

L

F

H

S

W

V

H

N

D

-

R

-

L

-

G

-

L

-

Q

-

D

-

V

-

A

T

R

N

S

T

V

L

G

D

D

D

F

K

V

V

L

L

H

V

R

K

A

N

D

D

G

G

L

M

W

P

A

T

Y
|
Y

Q

H

L

F

A

A

V
x
N

V

I

D

D

A

H

A

E

Q

M

G

K

I

I

T

T

D

H

V

V

V

I

R
|
R

G

G

E

E

D

E

L
x
W

A

L

D

P

N

S

T

M

P

A

R

L

Q

H

I

V

L

L

Q

Y

Q

E

A

A

L

M

G

G

V

W

P

D

T

A

P

P

R

E

Y

F

L

A

H

H

T

L

P

S

L

L

V

I

C

L

G

K

A

P

N

E

G

G

E

K

-

G

K

K

L

L

S

S

K
|
K

Q

R

N

D

G

G

A

D

R

K

active site: K261 (= K274)
binding glutamic acid: R6 (= R18), A8 (= A20), S10 (= S22), E42 (= E54), Y201 (= Y215), N205 (≠ V219), R219 (= R233), W223 (≠ L237)

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
31% identity, 80% coverage: 18:281/329 of query aligns to 5:260/485 of 4griB

query
sites
4griB

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

L

L

Q

H

H

A

I

G

G

S

G

L

I

V

R

A

T

A

A

L

L

A

F

S

N

W

Y

L

F

D

F

A

A

R

K

A

S

H

C

G

G

Q

K

W

F

L

L

V

L

R

R

I

I

E
|
E

D

D

V

T

D

D

T

Q

P

S

R

R

C

Y

V

S

P

P

G

E

A

A

D

E

Q

N

F

D

I

L

L

Y

Q

S

L

L

A

K

T

W

C

L

G

G

L

I

V

S

P

F

D

D

A

E

-

G

-

P

-

V

-

G

-

D

-

Y

P

A

P

P

E

Y

W

V

Q

Q

S

S

A

Q

R

R

S

S

A

A

H

I

Y

Y

Q

K

R

Q

A

Y

L

A

D

K

Q

Y

L

L

V

I

A

E

Q

S

G

G

H

H

A

A

Y

Y

P

Y

C
|
C

A

Y

C
|
C

S

S

R

P

K

E

D

R

V

L

E

E

D

R

A

I

Q

K

A

K

A

I

L

-

G

-

H

-

A

-

R

-

E

Q

R

N

H

I

A

N

A

K

L

M

P

P

Y

-

P

P

G

G

T
x
Y

C

D

R

R

H

H

-
x
C

-

R

G

N

L

L

R

S

G

N

R

E

T

E

G

V

R

E

S

N

W

A

R

L

F

I

N

K

A

K

T

I

D

K

F

P

K

V

Q

V

K

R

Q

F

A

K

L

I

P

P

P

L

D

E

G

G

Y

-

A

-

S

D

S

T

A

S

I

F

N

D

S

D

I

I

A

L

N

L

N

G

T

R

L

I

H

T

W

W

H

A

D

N

R

K

R

D

L

I

G

S

L

-

Q

-

D

-

V

-

A

-

R

-

S

-

V

-

G

P

D

D

F

P

V

V

L

I

H

L

R

K

A

S

D

D

G

G

L

L

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

Y

A

L

Q

M

G

K

I

I

T

T

D

H

V

V

V

L

R
|
R

G

A

E

Q

D

E

L
x
W

A

V

D

S

N

S

T

G

P

P

R

L

Q

H

I

V

L

L

Q

Y

Q

K

A

A

L

F

G

K

V

W

P

K

T

P

P

P

R

I

Y

Y

L

C

H

H

T

L

P

P

L

M

V

V

C

M

G

G

A

N

N

D

G

G

E

Q

K

K

L

L

S

S

K
|
K

Q

R

N

H

G

G

A

S

R

T

A

A

L

L

active site: S9 (= S22), K253 (= K274)
binding glutamic acid: R5 (= R18), A7 (= A20), S9 (= S22), E41 (= E54), Y194 (= Y215), R212 (= R233), W216 (≠ L237)
binding zinc ion: C105 (= C110), C107 (= C112), Y128 (≠ T139), C132 (vs. gap)

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 1g59A

query
sites
1g59A

I

V

G

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E

E

D

D

V

T

D
|
D

T
x
R

P
x
A

R
|
R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

A

-

P

-

T

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R
x
P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A
x
V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

T

L

T

H

E

W

V

H

K

D

D

R

E

R

L

L
x
R

G

G

L

V

Q
x
V

Q

V

Q

Y

D

D

V

-

A

N

R

Q

S
x
E

V

I

G

P

D

D

F

V

V
|
V

L

L

H

L

R
x
K

A
x
S

D
|
D

G

G

L

Y

W

P

A

T

Y

Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

I

V

T

T

D

D

V

V

V

I

R

R

G

A

E
|
E

D
x
E

L

W

A

L

D

V

N

S

T

T

P

P

R

I

Q

H

I

V

L

L

Q

Y

Q

R

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

R

Y

F

L

Y

H

H

T

M

P

P

L

L

V

L

C
x
R

G

N

A

P

N

D

G
x
K

E
x
T

K
|
K

L

I

S

S

K

K

Q

R

N

K

G

S

A

H

R

T

A

S

L

L

D

D

binding : D44 (= D57), R45 (≠ T58), A46 (≠ P59), R47 (= R60), P109 (≠ R114), V145 (≠ A156), R163 (≠ L190), V166 (≠ Q193), E172 (≠ S200), V177 (= V205), K180 (≠ R208), S181 (≠ A209), D182 (= D210), E207 (= E235), E208 (≠ D236), R237 (≠ C265), K241 (≠ G269), T242 (≠ E270), K243 (= K271)

Sites not aligning to the query:

binding : 273, 274, 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 2cv2A

query
sites
2cv2A

I

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S

T

L

A

V

Y

A
x
I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E
|
E

D

D

V
x
T

D
|
D

T

R

P

A

R
|
R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

G

-

P

-

H

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R

P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A
x
V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

T

L

T

H

E

W

V

H

K

D

D

R

E

R

L

L
x
R

G

G

L

V

Q

V

Q
x
Y

D

D

V

-

A

N

R

Q

S
x
E

V

I

G

P

D

D

F

V

V
|
V

L

L

H

L

R
x
K

A
x
S

D

D

G

G

L

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

I

V

T

T

D

D

V

V

V

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

A

L

D
x
V

N

S

T

T

P

P

R

I

Q

H

I

V

L

L

Q

Y

Q

R

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

R

Y

F

L

Y

H

H

T

M

P

P

L
|
L

V
x
L

C
x
R

G

N

A

P

N

D

G
x
K

E

T

K

K

L

I

S

S

K
|
K

Q

R

N

K

G

S

A

H

R

T

A

S

L

L

D

D

active site: K246 (= K274)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R18), A7 (= A20), S9 (= S22), G17 (= G30), I21 (≠ A34), E41 (= E54), Y187 (= Y215), R205 (= R233), A206 (≠ G234), E208 (≠ D236), W209 (≠ L237), L235 (= L263), L236 (≠ V264)
binding : S9 (= S22), T43 (≠ V56), D44 (= D57), R47 (= R60), V145 (≠ A156), R163 (≠ L190), Y168 (≠ Q195), E172 (≠ S200), V177 (= V205), K180 (≠ R208), S181 (≠ A209), Y187 (= Y215), E207 (= E235), E208 (≠ D236), W209 (≠ L237), V211 (≠ D239), R237 (≠ C265), K241 (≠ G269)

Sites not aligning to the query:

binding : 272, 273, 274, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448

2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 2cv1A

query
sites
2cv1A

I

V

G

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S
x
T

L

A

V

Y

A
x
I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E
|
E

D

D

V
x
T

D
|
D

T

R

P

A

R
|
R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

G

-

P

-

H

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R

P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A
x
V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

T

L

T

H

E

W

V

H

K

D

D

R

E

R

L

L
x
R

G

G

L

V

Q
x
V

Q

V

Q

Y

D

D

V

-

A

N

R

Q

S
x
E

V

I

G

P

D

D

F

V

V
|
V

L

L

H

L

R
x
K

A
x
S

D

D

G

G

L

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

I

V

T

T

D

D

V

V

V

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

A

L

D
x
V

N

S

T

T

P

P

R

I

Q

H

I

V

L

L

Q

Y

Q

R

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

R

Y

F

L

Y

H

H

T

M

P

P

L
|
L

V
x
L

C
x
R

G

N

A

P

N

D

G
x
K

E

T

K
|
K

L

I

S

S

K
|
K

Q

R

N

K

G

S

A

H

R

T

A

S

L

L

D

D

active site: K246 (= K274)
binding adenosine-5'-triphosphate: P8 (= P21), S9 (= S22), G17 (= G30), T18 (≠ S31), I21 (≠ A34), R47 (= R60), A206 (≠ G234), W209 (≠ L237), L235 (= L263), L236 (≠ V264)
binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R18), A7 (= A20), E41 (= E54), Y187 (= Y215), R205 (= R233), W209 (≠ L237)
binding : S9 (= S22), E41 (= E54), T43 (≠ V56), D44 (= D57), R47 (= R60), V145 (≠ A156), R163 (≠ L190), V166 (≠ Q193), E172 (≠ S200), V177 (= V205), K180 (≠ R208), S181 (≠ A209), Y187 (= Y215), E207 (= E235), E208 (≠ D236), W209 (≠ L237), V211 (≠ D239), R237 (≠ C265), K241 (≠ G269), K243 (= K271)

Sites not aligning to the query:

binding : 273, 274, 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 2cuzA

query
sites
2cuzA

I

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E
|
E

D

D

V

T

D

D

T

R

P

A

R

R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

G

-

P

-

H

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R

P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A

V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

T

L

T

H

E

W

V

H

K

D

D

R

E

R

L

L

R

G

G

L

V

Q

V

Q

Y

D

D

V

-

A

N

R

Q

S

E

V

I

G

P

D

D

F

V

V

V

L

L

H

L

R

K

A

S

D

D

G

G

L

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

I

V

T

T

D

D

V

V

V

I

R
|
R

G

A

E

E

D

E

L

W

A

L

D

V

N

S

T

T

P

P

R

I

Q

H

I

V

L

L

Q

Y

Q

R

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

R

Y

F

L

Y

H

H

T

M

P

P

L

L

V

L

C

R

G

N

A

P

N

D

G

K

E

T

K

K

L

I

S

S

K
|
K

Q

R

N

K

G

S

A

H

R

T

A

S

L

L

D

D

active site: K246 (= K274)
binding glutamic acid: R5 (= R18), A7 (= A20), S9 (= S22), E41 (= E54), Y187 (= Y215), R205 (= R233)

1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 1n78A

query
sites
1n78A

I

V

G

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S
x
T

L

A

V

Y

A
x
I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E
|
E

D

D

V
x
T

D
|
D

T

R

P

A

R
|
R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

G

-

P

-

H

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R

P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A
x
V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-

T

T

L

T

H

E

W

V

H

K

D

D

R

E

R

L

L
x
R

G

G

L

V

Q
x
V

Q

V

Q
x
Y

D

D

V

-

A

N

R

Q

S
x
E

V

I

G

P

D

D

F

V

V
|
V

L

L

H

L

R
x
K

A
x
S

D

D

G

G

L

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V
x
N

V

V

D

D

A

H

A

L

Q

M

G

G

I

V

T

T

D

D

V

V

V

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

A
x
L

D
x
V

N

S

T

T

P

P

R

I

Q

H

I

V

L

L

Q

Y

Q

R

A

A

L

F

G

G

V

W

P

E

T

A

P

P

R

R

Y

F

L

Y

H

H

T

M

P

P

L
|
L

V
x
L

C
x
R

G

N

A

P

N

D

G
x
K

E

T

K

K

L

I

S

S

K
|
K

Q

R

N

K

G

S

A

H

R

T

A

S

L

L

D

D

active site: K246 (= K274)
binding glutamol-amp: R5 (= R18), A7 (= A20), P8 (= P21), S9 (= S22), G17 (= G30), T18 (≠ S31), I21 (≠ A34), E41 (= E54), Y187 (= Y215), N191 (≠ V219), R205 (= R233), A206 (≠ G234), E208 (≠ D236), W209 (≠ L237), L235 (= L263), L236 (≠ V264)
binding : S9 (= S22), T43 (≠ V56), D44 (= D57), R47 (= R60), V145 (≠ A156), R163 (≠ L190), V166 (≠ Q193), Y168 (≠ Q195), E172 (≠ S200), V177 (= V205), K180 (≠ R208), S181 (≠ A209), Y187 (= Y215), E207 (= E235), E208 (≠ D236), W209 (≠ L237), L210 (≠ A238), V211 (≠ D239), R237 (≠ C265), K241 (≠ G269)

Sites not aligning to the query:

binding : 273, 274, 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
33% identity, 81% coverage: 16:282/329 of query aligns to 3:254/468 of 1j09A

query
sites
1j09A

I

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

D

L

P

H
|
H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

L

L

A

F

S

N

W

Y

L

A

D

W

A

A

R

R

A

R

H

N

G

G

Q

R

W

F

L

I

V

V

R

R

I

I

E
|
E

D

D

V

T

D

D

T

R

P

A

R

R

C

Y

V

V

P

P

G

G

A

A

D

E

Q

E

F

R

I

I

L

L

Q

A

L

L

A

K

T

W

C

L

G

G

L

L

V

S

P

Y

D

D

A

E

P

G

P

P

E

D

W

V

-

G

-

P

-

H

-

G

-

P

-

Y

-

R

Q

Q

S

S

A

E

R

R

S

L

A

P

H

L

Y

Y

Q

Q

R

K

A

Y

L

A

D

E

Q

E

L

L

V

L

A

K

Q

R

G

G

H

W

A

A

Y

Y

P

R

C

A

A

F

C

E

S

T

R

P

K

E

D

E

V

L

E

E

D

Q

A

I

Q

R

A

K

A

E

L

K

G

G

H

G

A

Y

R

D

E

G

R

R

H

A

A

R

A

N

L

I

P

P

Y

-

P

P

G

E

T

E

C

A

R

E

H

E

G

-

L

-

R

R

G

A

R

R

T

R

G

G

R

E

S

P

W

-

R

-

F

-

N

H

A

V

T

I

D

R

F

L

K

K

Q

-

K

-

Q

-

A

-

L

V

P

P

P

R

D

P

G

G

Y

-

A

-

S

-

A

-

I

-

N

-

S

-

I

-

A

-

N

-