SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS04860 FitnessBrowser__BFirm:BPHYT_RS04860 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 86% coverage: 10:250/280 of query aligns to 9:249/265 of P07821

query
sites
P07821

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K50 (= K51) mutation to Q: Lack of activity.
D172 (= D173) mutation to E: Lack of activity.
E173 (= E174) mutation to A: Lack of activity.

4fi3C Structure of vitamin b12 transporter btucd-f in a nucleotide-bound state (see paper)
35% identity, 85% coverage: 26:262/280 of query aligns to 13:245/248 of 4fi3C

query
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4fi3C

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binding phosphoaminophosphonic acid-adenylate ester: R14 (≠ L27), N34 (= N47), G35 (= G48), G37 (= G50), K38 (= K51), S39 (≠ T52), T40 (= T53), R121 (= R144), Q125 (≠ S148), S127 (= S150), G129 (= G152), E130 (= E153), Q158 (≠ E174)
binding magnesium ion: S39 (≠ T52), Q79 (= Q93)

1l7vC Bacterial abc transporter involved in b12 uptake (see paper)
37% identity, 79% coverage: 26:247/280 of query aligns to 13:230/231 of 1l7vC

query
sites
1l7vC

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binding cyclo-tetrametavanadate: N34 (= N47), G35 (= G48), A36 (= A49), G37 (= G50), K38 (= K51), S39 (≠ T52), T40 (= T53)

1yqtA Rnase-l inhibitor (see paper)
30% identity, 69% coverage: 37:230/280 of query aligns to 288:460/515 of 1yqtA

query
sites
1yqtA

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binding adenosine-5'-diphosphate: N298 (= N47), G299 (= G48), I300 (≠ A49), G301 (= G50), K302 (= K51), T303 (= T52), T304 (= T53)
binding magnesium ion: T303 (= T52), Q331 (= Q93)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 8, 13, 33, 34, 36, 37, 38, 39, 274, 277
binding magnesium ion: 38, 90

5yv5A Crystal structure of the complex of archaeal ribosomal stalk protein ap1 and archaeal ribosome recycling factor aabce1. (see paper)
30% identity, 69% coverage: 37:230/280 of query aligns to 291:463/517 of 5yv5A

query
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5yv5A

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binding adenosine-5'-diphosphate: N301 (= N47), G302 (= G48), I303 (≠ A49), G304 (= G50), K305 (= K51), T306 (= T52), T307 (= T53)
binding magnesium ion: T306 (= T52), Q334 (= Q93)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11, 14, 16, 36, 37, 39, 40, 41, 42, 277, 280
binding magnesium ion: 41, 93
binding : 94, 97, 98, 100, 101, 151, 152, 155

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
31% identity, 81% coverage: 38:264/280 of query aligns to 52:274/382 of 7ahhC

query
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7ahhC

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L

L

D

D

L

N

L

A

G

N

L

L

A

L

A

D

F

F

A

K

A

D

R

Q

D

Y

V

P

L

K

S

Q

L

L

S

S

G

G

E

M

R

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

A

R

V

A

L

L

C

A

Q

N

D

D

A

P

P

E

L

I

L

L

L

M

D
|
D

E
|
E

P

A

L

F

A

S

H

A

L

L

D

D

-

P

-

L

-

I

-

R

L

R

H

E

H

M

Q

Q

I

D

D

E

C

L

L

L

E

E

A

L

L

Q

A

A

A

K

W

F

T

-

R

-

A

-

P

-

R

Q

R

K

T

T

V

I

M

I

F

F

S

V

C

S

H

H

D

D

L

L

N

N

F

E

A

A

R

L

R

R

F

I

A

G

T

D

H

R

A

I

L

A

L

I

L

M

D

K

G

D

A

G

G

K

G

I

A

M

Y

Q

A

I

G

G

P

T

V

G

H

E

D

E

V

I

L

L

T

T

P

-

T

-

L

-

A

-

S

N

R

P

A

A

F

N

G

D

Y

Y

P

V

L

K

L

T

L

F

I

V

R

E

D

D

G

T

E

A

H

E

E

N

A

I

L

M

I

I

P

P

A

A

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G48), G64 (= G50), K65 (= K51), D187 (= D173), E188 (= E174)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
31% identity, 81% coverage: 38:264/280 of query aligns to 52:274/382 of 7aheC

query
sites
7aheC

G

G

E

E

I

I

W

F

C

V

I

I

A

M

G

G

P

L

N

S

G

G

A

S

G

G

K

K

T

S

T

T

L

L

S

R

T

L

L

L

A

N

G

R

L

L

L

I

Q

E

P

P

S

T

A

S

G

G

H

K

V

I

E

F

L

I

D

D

G

N

V

Q

R

D

L

V

A

A

D

T

W

L

P

N

P

K

L

E

S

D

L

L

A

L

Q

Q

-

V

R

R

A

K

L

T

M

M

P

S

Q

M

S

-

A

-

P

-

D

-

A

V

F

F

S

Q

A

N

S

F

V

G

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

F

P

P

H

H

L

R

T

T

-

I

-

L

-

E

-

N

-

T

G

E

W

Y

G

G

W

L

E

E

-

V

-

Q

-

N

-

V

-

P

R

K

E

E

A

E

D

R

R

R

E

K

A

R

A

A

H

E

A

K

A

A

L

L

D

D

L

N

L

A

G

N

L

L

A

L

A

D

F

F

A

K

A

D

R

Q

D

Y

V

P

L

K

S

Q

L

L

S

S

G

G

E

M

R

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

A

R

V

A

L

L

C

A

Q

N

D

D

A

P

P

E

L

I

L

L

L

M

D

D

E

E

P

A

L

F

A

S

H

A

L

L

D

D

-

P

-

L

-

I

-

R

L

R

H

E

H

M

Q

Q

I

D

D

E

C

L

L

L

E

E

A

L

L

Q

A

A

A

K

W

F

T

-

R

-

A

-

P

-

R

Q

R

K

T

T

V

I

M

I

F

F

S

V

C

S

H

H

D

D

L

L

N

N

F

E

A

A

R

L

R

R

F

I

A

G

T

D

H

R

A

I

L

A

L

I

L

M

D

K

G

D

A

G

G

K

G

I

A

M

Y

Q

A

I

G

G

P

T

V

G

H

E

D

E

V

I

L

L

T

T

P

-

T

-

L

-

A

-

S

N

R

P

A

A

F

N

G

D

Y

Y

P

V

L

K

L

T

L

F

I

V

R

E

D

D

G

T

E

A

H

E

E

N

A

I

L

M

I

I

P

P

A

A

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

4hluC Structure of the ecfa-a' heterodimer bound to adp (see paper)
32% identity, 69% coverage: 18:211/280 of query aligns to 10:195/249 of 4hluC

query
sites
4hluC

L

V

T

S

L

F

R

R

A
x
Y

G

N

A

G

R

D

T

Y

L

V

L

L

D

K

A

D

F

V

T

N

H

A

T

E

F

F

Y

E

A

T

G

G

E

K

I

I

W

Y

C

V

I

V

A

V

G

G

P

K

N
|
N

G
|
G

A

S

G
|
G

K
|
K

T
|
T

L

L

S

K

T

I

L

L

A

A

G

G

L

L

Q

-

P

A

S

A

A

A

G

G

H

E

V

I

E

F

L

L

D

D

G

G

V

-

R

-

L

-

A

S

D

P

W

A

P

D

P

P

L

F

S

L

L

L

A

R

Q

K

R

N

R

V

A

G

L

Y

M

V

P

F

Q

Q

S

N

-

P

A

S

P

S

D

Q

A

I

F

I

S

G

A

A

S

T

V

V

L

E

D

E

I

D

V

V

M

A

L

F

N

S

R

-

F

-

P

-

H

-

L

L

T

E

G

I

W

M

G

G

W

L

E

D

R

E

E

S

A

E

D

M

R

R

E

K

A

R

A

I

H

K

A

K

A

V

L

L

D

E

L

L

L

V

G

G

L

L

A

S

A

G

F

L

A

A

R

A

D

D

V

P

L

L

S

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

L

A

A

L

I

A

A

A

S

V

M

L

L

C

A

Q

R

D

D

A

T

P

R

L

F

L

L

L

A

L

L

D

D

E

E

P

P

L

V

A

S

H

M

L

L

D

D

L

P

H

P

H

S

Q

Q

I

R

D

E

C

I

L

F

E

Q

A

V

L

L

A

E

A

S

W

-

T

L

R

K

A

N

P

E

R

G

R

K

T

G

V

I

M

I

F

L

S

V

C

T

H

H

D

E

L

L

N

E

F

Y

binding adenosine-5'-diphosphate: Y14 (≠ A22), N39 (= N47), G40 (= G48), G42 (= G50), K43 (= K51), T44 (= T52), T45 (= T53)

4hluA Structure of the ecfa-a' heterodimer bound to adp (see paper)
29% identity, 81% coverage: 28:254/280 of query aligns to 23:242/265 of 4hluA

query
sites
4hluA

L

L

D

E

A

N

F

V

T

S

H

L

T

V

F

I

Y

N

A

E

G

G

E

E

I

C

W

L

C

L

I

V

A

A

G

G

P

N

N

T

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

Q

T

I

L

V

A

A

G

G

L

L

L

I

Q

E

P

P

S

T

A

S

G

G

H

D

V

V

E

L

L

Y

D

D

G

G

V

E

R

R

L

K

A

K

D

G

W

Y

P

-

L

-

S

E

L

I

A

R

Q

R

R

N

R

I

A

G

L

I

M

A

P

F

Q

Q

S

Y

A

P

P

E

D

D

A

Q

F

F

S

F

A

A

S

E

-

R

V

V

L

F

D

D

I

E

V

V

-

A

-

F

-

A

M

V

L

K

N

N

R

F

F

Y

P

P

H

-

L

-

T

-

G

-

W

-

G

-

W

-

E

-

R

-

E

-

A

-

D

D

R

R

E

D

-

P

-

V

-

P

A

L

A

V

H

K

A

K

A

A

L

M

D

E

L

F

L

V

G

G

L

L

A

D

-

F

-

D

A

S

F

F

A

K

A

D

R

R

D

V

V

P

L

F

S

F

L

L

S

S

G

G

E

E

R

K

Q

R

R

R

V

V

A

A

L

I

A

A

A

S

V

V

L

I

C

V

Q

H

D

E

A

P

P

D

L

I

L

L

L

I

L

L

D

D

E

E

P

P

L

L

A

V

H

G

L

L

D

D

L

R

H

E

H

G

Q

K

I

T

D

D

C

L

L

L

E

R

A

I

L

V

A

E

A

K

W

W

T

-

R

K

A

T

P

L

R

G

R

K

T

T

V

V

M

I

F

L

S

I

C

S

H

H

D

D

L

I

N

E

F

T

A

V

R

I

R

N

F

H

A

V

T

D

H

R

A

V

L

V

L

L

D

E

G

K

A

G

G

K

A

V

Y

F

A

D

G

G

P

T

V

R

H

M

D

E

V

F

L

L

T

E

P

-

T

K

L

Y

A

D

S

P

R

R

A

F

F

F

G

T

Y

S

P

K

L

M

L

L

L

V

I

M

R

R

binding adenosine-5'-diphosphate: G43 (= G48), S44 (≠ A49), G45 (= G50), K46 (= K51), S47 (≠ T52), T48 (= T53)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 20

3d31A Modbc from methanosarcina acetivorans (see paper)
33% identity, 71% coverage: 28:225/280 of query aligns to 16:205/348 of 3d31A

query
sites
3d31A

L

L

D

D

A

N

F

L

T

S

H

L

T

K

F

V

Y

E

A

S

G

G

E

E

I

Y

W

F

C

V

I

I

A

L

G

G

P

P

N

T

G

G

A

A

G

G

K

K

T

T

T

L

L

F

L

L

S

E

T

L

L

I

A

A

G

G

L

F

L

H

Q

V

P

P

S

D

A

S

G

G

H

R

V

I

E

L

L

L

D

D

G

G

V

K

R

D

L

V

A

T

D

D

W

-

P

-

L

-

S

-

L

-

A

-

Q

-

R

-

A

-

L

L

M

S

P

P

Q

E

S

K

A

H

P

D

D

I

A

A

F

F

S

V

A

Y

S

Q

V

N

L

Y

D

S

I

L

V

-

M

-

L

-

N

-

R

-

F

F

P

P

H

H

L

M

T

N

-

V

-

K

-

N

-

L

-

E

-

F

G

G

W

M

G

R

W

M

E

K

R

K

E

I

A

K

D

D

-

P

R

K

E

R

A

V

A

L

H

D

A

T

A

A

L

R

D

D

L

L

L

-

G

K

L

I

A

E

A

H

F

L

A

L

A

D

R

R

D

N

V

P

L

L

S

T

L

L

S

S

G

G

E

E

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

A

R

V

A

L

L

C

V

Q

T

D

N

A

P

P

K

L

I

L

L

D

D

E

E

P

P

L

L

A

S

H

A

L

L

D

D

L

P

H

R

H

T

Q

Q

I

E

D

N

C

A

L

R

E

E

A

M

L

L

A

S

A

V

W

L

T

H

R

K

A

K

P

N

R

K

R

L

T

T

V

V

M

L

F

H

S

I

C

T

H

H

D

D

L

Q

N

T

F

E

A

A

R

R

R

I

F

M

A

A

T

D

H

R

-

I

A

A

L

V

L

M

D

D

G

G

A

K

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

3bk7A Structure of the complete abce1/rnaase-l inhibitor protein from pyrococcus abysii (see paper)
31% identity, 68% coverage: 37:227/280 of query aligns to 367:536/593 of 3bk7A

query
sites
3bk7A

A

K

G

G

E

E

I

V

W

I

C

G

I

I

A

V

G

G

P

P

N
|
N

G
|
G

A
x
I

G
|
G

K
|
K

T
|
T

L

F

L

V

S

K

T

M

L

L

A

A

G

G

L

V

L

E

Q

E

P

P

S

T

A

E

G

G

H

K

V

V

E

E

L

W

D

D

G

-

V

-

R

-

L

-

A

-

D

-

W

-

P

-

L

L

S

T

L

V

A

A

Q

Y

R

K

R

-

A

-

L

-

M

-

P

P

Q

Q

S

Y

A

I

P

K

D

A

A

E

F

Y

S

E

A

G

S

T

V

V

L

Y

D

E

I

L

V

L

M

S

L

K

N

I

R

D

F

S

P

S

H

K

L

L

T

N

G

S

W

N

G

F

W

Y

E

K

R

T

E

E

A

L

D

-

R

-

E

-

A

-

H

-

A

-

L

L

D

K

L

P

L

L

G

G

L

I

A

I

A

D

F

L

A

Y

A

D

R

R

D

N

V

V

L

E

S

D

L

L

S

S

G

G

E

E

R

L

Q

Q

R

R

V

V

A

A

L

I

A

A

V

T

L

L

C

L

Q

R

D

D

A

A

P

D

L

I

L

Y

L

L

D

D

E

E

P

P

L

S

A

A

H

Y

L

L

D

D

L

V

H

E

H

Q

Q

R

I

L

D

A

C

V

L

S

E

R

A

A

L

I

A

R

A

H

W

L

T

M

R

E

A

K

P

N

R

E

R

K

T

T

V

A

M

L

F

V

S

V

C

E

H

H

D

D

L

V

N

L

F

M

A

I

R

D

R

Y

F

V

A

S

T

D

H

R

A

L

L

I

L

V

L

F

D

E

G

G

A

E

G

P

G

G

binding adenosine-5'-diphosphate: N377 (= N47), G378 (= G48), I379 (≠ A49), G380 (= G50), K381 (= K51), T382 (= T52), T383 (= T53)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 87, 92, 112, 113, 114, 115, 116, 117, 118, 292, 353
binding iron/sulfur cluster: 15, 16, 20, 25, 29, 30, 38, 39, 55, 56, 57, 58, 59, 61, 65, 70

4zirA Crystal structure of ecfaa' heterodimer bound to amppnp (see paper)
28% identity, 81% coverage: 28:254/280 of query aligns to 23:240/263 of 4zirA

query
sites
4zirA

L

L

D

E

A

N

F

V

T

S

H

L

T

V

F

I

Y

N

A

E

G

G

E

E

I

C

W

L

C

L

I

V

A

A

G

G

P

N

N
x
T

G

G

A

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

Q

T

I

L

V

A

A

G

G

L

L

L

I

Q

E

P

P

S

T

A

S

G

G

H

D

V

V

E

L

L

Y

D

D

G

G

V

E

R

K

L

-

A

-

D

-

W

-

P

-

P

G

L

Y

S

E

L

I

A

R

Q

R

R

N

R

I

A

G

L

I

M

A

P

F

Q
|
Q

S

Y

A

P

P

E

D

D

A

Q

F

F

S

F

A

A

S

E

-

R

V

V

L

F

D

D

I

E

V

V

-

A

-

F

-

A

M

V

L

K

N

N

R

F

F

Y

P

P

H

-

L

-

T

-

G

-

W

-

G

-

W

-

E

-

R

-

E

-

A

-

D

D

R

R

E

D

-

P

-

V

-

P

A

L

A

V

H

K

A

K

A

A

L

M

D

E

L

F

L

V

G

G

L

L

A

D

-

F

-

D

A

S

F

F

A

K

A

D

R
|
R

D

V

V

P

L

F

S
x
F

L

L

S
|
S

G
|
G

E
|
E

R

K

Q

R

R

R

V

V

A

A

L

I

A

A

A

S

V

V

L

I

C

V

Q

H

D

E

A

P

P

D

L

I

L

L

L

I

L

L

D
|
D

E

E

P

P

L

L

A

V

H

G

L

L

D

D

L

R

H

E

H

G

Q

K

I

T

D

D

C

L

L

L

E

R

A

I

L

V

A

E

A

K

W

W

T

-

R

K

A

T

P

L

R

G

R

K

T

T

V

V

M

I

F

L

S

I

C

S

H

H

D

D

L

I

N

E

F

T

A

V

R

I

R

N

F

H

A

V

T

D

H

R

A

V

L

V

L

L

D

E

G

K

A

G

G

K

A

V

Y

F

A

D

G

G

P

T

V

R

H

M

D

E

V

F

L

L

T

E

P

-

T

K

L

Y

A

D

S

P

R

R

A

F

F

F

G

T

Y

S

P

K

L

M

L

L

L

V

I

M

R

R

binding phosphoaminophosphonic acid-adenylate ester: T42 (≠ N47), G45 (= G50), K46 (= K51), S47 (≠ T52), T48 (= T53), Q83 (= Q93), R132 (= R144), F136 (≠ S148), S138 (= S150), G139 (= G151), G140 (= G152), E141 (= E153)
binding magnesium ion: S47 (≠ T52), Q83 (= Q93), D161 (= D173)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

7ahdC Opua (e190q) occluded (see paper)
32% identity, 72% coverage: 38:238/280 of query aligns to 52:252/260 of 7ahdC

query
sites
7ahdC

G

G

E

E

I

I

W

F

C

V

I

I

A

M

G

G

P

L

N
x
S

G
|
G

A

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

T

L

L

L

A

N

G

R

L

L

L

I

Q

E

P

P

S

T

A

S

G

G

H

K

V

I

E

F

L

I

D

D

G

N

V

Q

R

D

L

V

A

A

D

T

W

L

P

N

P

K

L

E

S

D

L

L

A

L

Q

Q

-

V

R

R

A

K

L

T

M

M

P

S

Q

M

S

-

A

-

P

-

D

-

A

V

F

F

S
x
Q

A

N

S

F

V

G

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

F

P

P

H

H

L

R

T

T

-

I

-

L

-

E

-

N

-

T

G

E

W

Y

G

G

W

L

E

E

-

V

-

Q

-

N

-

V

-

P

R

K

E

E

A

E

D

R

R

R

E

K

A

R

A

A

H

E

A

K

A

A

L

L

D

D

L

N

L

A

G

N

L

L

A

L

A

D

F

F

A

K

A

D

R

Q

D

Y

V

P

L
x
K

S
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
M

R

Q

Q

Q

R

R

V

V

A

G

L

L

A

A

A

R

V

A

L

L

C

A

Q

N

D

D

A

P

P

E

L

I

L

L

L

M

D

D

E
x
Q

P

A

L

F

A

S

H

A

L

L

D

D

-

P

-

L

-

I

-

R

L

R

H

E

H

M

Q

Q

I

D

D

E

C

L

L

L

E

E

A

L

L

Q

A

A

A

K

W

F

T

-

R

-

A

-

P

-

R

Q

R

K

T

T

V

I

M

I

F

F

S

V

C

S

H
|
H

D

D

L

L

N

N

F

E

A

A

R

L

R

R

F

I

A

G

T

D

H

R

A

I

L

A

L

I

L

M

D

K

G

D

A

G

G

K

G

I

A

M

Y

Q

A

I

G

G

P

T

V

G

H

E

D

E

V

I

L

L

T

T

binding adenosine-5'-triphosphate: S61 (≠ N47), G62 (= G48), G64 (= G50), K65 (= K51), S66 (≠ T52), T67 (= T53), Q111 (≠ S100), K161 (≠ L147), Q162 (≠ S148), S164 (= S150), G166 (= G152), M167 (≠ E153), Q188 (≠ E174), H221 (= H207)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

6tejB Structure of apo irtab devoid sid in complex with sybody syb_nl5 (see paper)
34% identity, 65% coverage: 23:205/280 of query aligns to 341:522/585 of 6tejB

query
sites
6tejB

G

G

A

D

R
x
E

T

V

L

V

L

L

D

D

A

G

F

V

T

S

H

F

T

T

F

L

Y

R

A

P

G

G

E

N

I

T

W

T

C

A

I

I

A

V

G

G

P

P

N

S

G

G

A

S

G

G

K

K

T
|
T

T

T

L

I

L
|
L

S

S

T

L

L

I

A

A

G

G

L

L

L
x
Q

Q

Q

P

P

S

A

A

S

G

G

H

R

V

V

E

L

L

L

D

D

G

G

V

V

R

D

L

V

A

T

D

T

W

L

P

D

P

P

L

E

S

A

L

R

A

R

Q

A

R

A

R

V

A

S

L

V

M

V

P
x
F

Q

Q

S

H

A

-

P

P

D

Y

A

L

F

F

S

D

A

G

S

T

V

L

L

R

D

D

I

N

V

V

M

L

L

V

N

G

R

-

F

-

P

-

H

-

L

-

T

-

G

-

W

-

G

-

W

-

E

D

R

P

E

E

A

A

D

D

R

P

E

D

A

D

A

V

H

T

A

A

L

M

D

R

L

L

L

A

-

R

-

V

-

D

-

E

-

L

-

D

-

R

-

L

-

P

-

D

G

G

L

D

A

A

A

T

F

V

A

V

A

G

R

E

D

G

V

G

L

T

S

A

L

L

S

S

G

G

E

E

R

R

Q

Q

R

R

V

V

A

S

L

I

A

A

A

R

V

A

L

L

C

L

Q

K

D

P

A

A

P

P

L

V

L

L

L

V

D

D

E

E

P

A

L

T

A

S

H

A

L

L

D

D

L

N

H

A

H

N

Q

E

I

A

D

A

C

V

L

V

E

D

A

A

L

L

A

T

A

A

W

D

T

P

R

R

A

-

P

P

R

R

R

T

T

R

V

V

M

I

F

V

S

A

binding decyl-beta-d-maltopyranoside: E343 (≠ R25), T370 (= T52), L373 (= L55), Q380 (≠ L62), F410 (≠ P92)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 340

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
27% identity, 75% coverage: 38:248/280 of query aligns to 28:235/241 of 4u00A

query
sites
4u00A

G

G

E

E

I

K

W

L

C

V

I

I

A

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

S

R

T

T

L

I

A

N

G

R

L

L

L

E

Q

D

P

F

S

Q

A

E

G

G

H

E

V

V

E

V

L

V

D

D

G

G

V

L

R

S

L

V

A

K

D

D

W

D

P

R

P

A

L

L

S

R

L

E

A

I

Q

R

R

R

R

E

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S

Q

A

Q

S

-

V

-

L

-

D

-

I

-

V

-

M

-

L

F

N

N

R

L

F

F

P

P

H

H

L

M

T

T

G

V

W

L

G

E

-

N

-

V

-

T

-

L

-

A

-

P

-

M

-

R

-

V

-

R

W

W

E

P

R

R

E

E

A

K

D

A

R

E

E

K

A

K

A

A

H

L

A

E

A

L

L

L

D

E

L

R

L

V

G

G

L

I

A

L

A

D

F

Q

A

A

A

R

R

K

D

Y

V

P

L

A

S

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

A

L

I

A

A

A

R

V

A

L

L

C

A

Q

M

D

E

A

P

P

K

L

I

L

M

L

L

L

F

D

D

E

E

P

P

L

T

A

S

H

A

L

L

D

D

L

P

H

E

H

M

Q

V

I

G

D

E

C

V

L

L

E

D

A

V

L

M

A

R

A

D

W

L

T

A

R

Q

A

G

P

G

R

-

R

M

T

T

V

M

M

V

F

V

S

V

C

T

H

H

D

E

L

M

N

G

F

F

A

A

R

R

R

E

F

V

A

A

T

D

H

R

A

V

L

V

L

F

L

M

D

D

G

G

A

G

G

Q

G

I

A

V

Y

E

A

E

G

G

P

R

V

P

H

E

D

E

V

I

L

F

T

T

P

R

T

P

L

K

A

E

S

E

R

R

A

T

F

R

G

S

Y

F

binding adenosine-5'-diphosphate: S37 (≠ N47), G38 (= G48), S39 (≠ A49), G40 (= G50), K41 (= K51), S42 (≠ T52), T43 (= T53)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
28% identity, 79% coverage: 11:231/280 of query aligns to 1:215/374 of 2awnB

query
sites
2awnB

A

A

T

S

L

V

G

Q

A

L

Q

Q

R

N

L

V

T

T

L

K

R

A

A
x
W

G

G

A

E

R

V

T

V

L
x
V

L

S

D

K

A

D

F

I

T

N

H

L

T

D

F

I

Y

H

A

E

G

G

E

E

I

F

W

V

C

V

I

F

A

V

G

G

P

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

T

M

L

I

A

A

G

G

L

L

L

E

Q

T

P

I

S

T

A

S

G

G

H

D

V

L

E

F

L

I

D

G

G

E

V

K

R

R

L

M

A

N

D

D

W

T

P

P

L

-

S

-

L

-

A

A

Q

E

R

R

R

G

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S

Q

A

S

S

Y

V

A

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

Y

P

P

H

H

L

L

T

S

G

V

W

A

-

E

-

N

-

M

-

S

-

F

-

G

-

L

-

K

-

L

-

A

G

G

W

A

E

K

R

K

E

E

A

V

D

I

R

N

E

Q

A

R

A

V

H

N

A

Q

A

V

L

A

D

E

L

V

L

L

G

Q

L

L

A

A

A

H

F

L

A

L

A

D

R

R

D

K

V

P

L

K

S

A

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

A

R

V

T

L

L

C

V

Q

A

D

E

A

P

P

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

A

S

H

N

L

L

D

D

L

A

H

A

H

L

Q

R

I

V

D

Q

C

M

L

R

E

I

A

E

L

I

A

S

A

R

W

L

T

H

R

K

A

R

P

L

R

G

R

R

T

T

V

M

M

I

F

Y

S

V

C

T

H

H

D

D

L

Q

N

V

F

E

A

A

R

M

R

T

F

L

A

A

T

D

H

K

A

I

L

V

L

L

D

D

G

A

A

G

G

R

G

V

A

A

Y

Q

A

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ A22), V17 (≠ L27), S37 (≠ N47), G38 (= G48), G40 (= G50), K41 (= K51), S42 (≠ T52), T43 (= T53)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
28% identity, 79% coverage: 11:231/280 of query aligns to 2:216/371 of P68187

query
sites
P68187

A

A

T

S

L

V

G

Q

A

L

Q

Q

R

N

L

V

T

T

L

K

R

A

A

W

G

G

A

E

R

V

T

V

L

V

L

S

D

K

A

D

F

I

T

N

H

L

T

D

F

I

Y

H

A

E

G

G

E

E

I

F

W

V

C

V

I

F

A

V

G

G

P

P

N

S

G

G

A

C

G

G

K

K

T

S

T

T

L

L

S

R

T

M

L

I

A

A

G

G

L

L

L

E

Q

T

P

I

S

T

A

S

G

G

H

D

V

L

E

F

L

I

D

G

G

E

V

K

R

R

L

M

A

N

D

D

W

T

P

P

L

-

S

-

L

-

A

A

Q

E

R

R

R

G

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S

Q

A

S

S

Y

V
x
A

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

Y

P

P

H

H

L

L

T

S

G

V

W

A

-

E

-

N

-

M

-

S

-

F

-

G

-

L

-

K

-

L

-

A

G

G

W

A

E
x
K

R

K

E

E

A

V

D

I

R

N

E

Q

A

R

A
x
V

H

N

A

Q

A
x
V

L

A

D
x
E

L

V

L

L

G

Q

L

L

A
|
A

A

H

F

L

A

L

A

D

R

R

D

K

V

P

L

K

S

A

L

L

S

S

G

G

G
|
G

E

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

A

R

V

T

L

L

C

V

Q

A

D

E

A

P

P

S

L

V

L

F

L

L

D
|
D

E

E

P

P

L

L

A

S

H

N

L

L

D

D

L

A

H

A

H

L

Q

R

I

V

D

Q

C

M

L

R

E

I

A

E

L

I

A

S

A

R

W

L

T

H

R

K

A

R

P

L

R

G

R

R

T

T

V

M

M

I

F

Y

S

V

C

T

H

H

D

D

L

Q

N

V

F

E

A

A

R

M

R

T

F

L

A

A

T

D

H

K

A

I

L

V

L

L

D

D

G

A

A

G

G

R

G

V

A

A

Y

Q

A

V

G

G

A85 (≠ V103) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ E121) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A129) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A132) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ D134) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A139) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G152) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D173) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
28% identity, 79% coverage: 11:231/280 of query aligns to 1:215/371 of 3puyA

query
sites
3puyA

A

A

T

S

L

V

G

Q

A

L

Q

Q

R

N

L

V

T

T

L

K

R

A

A
x
W

G

G

A

E

R

V

T

V

L

V

L

S

D

K

A

D

F

I

T

N

H

L

T

D

F

I

Y

H

A

E

G

G

E

E

I

F

W

V

C

V

I

F

A

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

T

M

L

I

A

A

G

G

L

L

L

E

Q

T

P

I

S

T

A

S

G

G

H

D

V

L

E

F

L

I

D

G

G

E

V

K

R

R

L

M

A

N

D

D

W

T

P

P

L

-

S

-

L

-

A

A

Q

E

R

R

R

G

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S
x
Q

A

S

S

Y

V

A

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

Y

P

P

H

H

L

L

T

S

G

V

W

A

-

E

-

N

-

M

-

S

-

F

-

G

-

L

-

K

-

L

-

A

G

G

W

A

E

K

R

K

E

E

A

V

D

I

R

N

E

Q

A

R

A

V

H

N

A

Q

A

V

L

A

D

E

L

V

L

L

G

Q

L

L

A

A

A

H

F

L

A

L

A

D

R
|
R

D

K

V

P

L

K

S
x
A

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

A

R

V

T

L

L

C

V

Q

A

D

E

A

P

P

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

A

S

H

N

L

L

D

D

L

A

H

A

H

L

Q

R

I

V

D

Q

C

M

L

R

E

I

A

E

L

I

A

S

A

R

W

L

T

H

R

K

A

R

P

L

R

G

R

R

T

T

V

M

M

I

F

Y

S

V

C

T

H
|
H

D

D

L

Q

N

V

F

E

A

A

R

M

R

T

F

L

A

A

T

D

H

K

A

I

L

V

L

L

D

D

G

A

A

G

G

R

G

V

A

A

Y

Q

A

V

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ A22), S37 (≠ N47), G38 (= G48), C39 (≠ A49), G40 (= G50), K41 (= K51), S42 (≠ T52), T43 (= T53), Q81 (≠ S100), R128 (= R144), A132 (≠ S148), S134 (= S150), G136 (= G152), Q137 (≠ E153), E158 (= E174), H191 (= H207)
binding magnesium ion: S42 (≠ T52), Q81 (≠ S100)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
28% identity, 79% coverage: 11:231/280 of query aligns to 1:215/371 of 3puxA

query
sites
3puxA

A

A

T

S

L

V

G

Q

A

L

Q

Q

R

N

L

V

T

T

L

K

R

A

A
x
W

G

G

A

E

R

V

T

V

L

V

L

S

D

K

A

D

F

I

T

N

H

L

T

D

F

I

Y

H

A

E

G

G

E

E

I

F

W

V

C

V

I

F

A

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

T

M

L

I

A

A

G

G

L

L

L

E

Q

T

P

I

S

T

A

S

G

G

H

D

V

L

E

F

L

I

D

G

G

E

V

K

R

R

L

M

A

N

D

D

W

T

P

P

L

-

S

-

L

-

A

A

Q

E

R

R

R

G

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S
x
Q

A

S

S

Y

V

A

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

Y

P

P

H

H

L

L

T

S

G

V

W

A

-

E

-

N

-

M

-

S

-

F

-

G

-

L

-

K

-

L

-

A

G

G

W

A

E

K

R

K

E

E

A

V

D

I

R

N

E

Q

A

R

A

V

H

N

A

Q

A

V

L

A

D

E

L

V

L

L

G

Q

L

L

A

A

A

H

F

L

A

L

A

D

R
|
R

D

K

V

P

L

K

S

A

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

A

R

V

T

L

L

C

V

Q

A

D

E

A

P

P

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

A

S

H

N

L

L

D

D

L

A

H

A

H

L

Q

R

I

V

D

Q

C

M

L

R

E

I

A

E

L

I

A

S

A

R

W

L

T

H

R

K

A

R

P

L

R

G

R

R

T

T

V

M

M

I

F

Y

S

V

C

T

H
|
H

D

D

L

Q

N

V

F

E

A

A

R

M

R

T

F

L

A

A

T

D

H

K

A

I

L

V

L

L

D

D

G

A

A

G

G

R

G

V

A

A

Y

Q

A

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ A22), G38 (= G48), C39 (≠ A49), G40 (= G50), K41 (= K51), S42 (≠ T52), T43 (= T53), R128 (= R144), S134 (= S150), Q137 (≠ E153)
binding beryllium trifluoride ion: S37 (≠ N47), G38 (= G48), K41 (= K51), Q81 (≠ S100), S134 (= S150), G136 (= G152), H191 (= H207)
binding magnesium ion: S42 (≠ T52), Q81 (≠ S100)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
28% identity, 79% coverage: 11:231/280 of query aligns to 1:215/371 of 3puwA

query
sites
3puwA

A

A

T

S

L

V

G

Q

A

L

Q

Q

R

N

L

V

T

T

L

K

R

A

A
x
W

G

G

A

E

R

V

T

V

L
x
V

L

S

D

K

A

D

F

I

T

N

H

L

T

D

F

I

Y

H

A

E

G

G

E

E

I

F

W

V

C

V

I

F

A

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

S

R

T

M

L

I

A

A

G

G

L

L

L

E

Q

T

P

I

S

T

A

S

G

G

H

D

V

L

E

F

L

I

D

G

G

E

V

K

R

R

L

M

A

N

D

D

W

T

P

P

L

-

S

-

L

-

A

A

Q

E

R

R

R

G

A

V

L

G

M

M

P

-

Q

-

S

-

A

-

P

-

D

-

A

V

F

F

S
x
Q

A

S

S

Y

V

A

L

L

D

-

I

-

V

-

M

-

L

-

N

-

R

-

F

Y

P

P

H

H

L

L

T

S

G

V

W

A

-

E

-

N

-

M

-

S

-

F

-

G

-

L

-

K

-

L

-

A

G

G

W

A

E

K

R

K

E

E

A

V

D

I

R

N

E

Q

A

R

A

V

H

N

A

Q

A

V

L

A

D

E

L

V

L

L

G

Q

L

L

A

A

A

H

F

L

A

L

A

D

R
|
R

D

K

V

P

L

K

S
x
A

L

L

S
|
S

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

A

R

V

T

L

L

C

V

Q

A

D

E

A

P

P

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

A

S

H

N

L

L

D

D

L

A

H

A

H

L

Q

R

I

V

D

Q

C

M

L

R

E

I

A

E

L

I

A

S

A

R

W

L

T

H

R

K

A

R

P

L

R

G

R

R

T

T

V

M

M

I

F

Y

S

V

C

T

H
|
H

D

D

L

Q

N

V

F

E

A

A

R

M

R

T

F

L

A

A

T

D

H

K

A

I

L

V

L

L

D

D

G

A

A

G

G

R

G

V

A

A

Y

Q

A

V

G

G

binding adenosine-5'-diphosphate: W12 (≠ A22), V17 (≠ L27), G38 (= G48), C39 (≠ A49), G40 (= G50), K41 (= K51), S42 (≠ T52), T43 (= T53), R128 (= R144), A132 (≠ S148), S134 (= S150), Q137 (≠ E153)
binding tetrafluoroaluminate ion: S37 (≠ N47), G38 (= G48), K41 (= K51), Q81 (≠ S100), S134 (= S150), G135 (= G151), G136 (= G152), E158 (= E174), H191 (= H207)
binding magnesium ion: S42 (≠ T52), Q81 (≠ S100)

Query Sequence

>BPHYT_RS04860 FitnessBrowser__BFirm:BPHYT_RS04860
MQTRHAPSHDATLGAQRLTLRAGARTLLDAFTHTFYAGEIWCIAGPNGAGKTTLLSTLAG
LLQPSAGHVELDGVRLADWPPLSLAQRRALMPQSAPDAFSASVLDIVMLNRFPHLTGWGW
EREADREAAHAALDLLGLAAFAARDVLSLSGGERQRVALAAVLCQDAPLLLLDEPLAHLD
LHHQIDCLEALAAWTRAPRRTVMFSCHDLNFARRFATHALLLDGAGGAYAGPVHDVLTPT
LASRAFGYPLLLIRDGEHEALIPAPRARHESPAGHDAPAS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory