SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS12000 FitnessBrowser__BFirm:BPHYT_RS12000 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

4ru1A Crystal structure of carbohydrate transporter acei_1806 from acidothermus cellulolyticus 11b, target efi-510965, in complex with myo-inositol
31% identity, 87% coverage: 41:347/351 of query aligns to 9:283/288 of 4ru1A

query
sites
4ru1A

L

M

V

I

S

T

H
|
H

A

Q

P

Q

D

P

S

G

D
|
D

T

T

F

F

W
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W

N

D

T

I

I

I

R

R

N

K

A

G

I

A

E

L

Q

A

A

A

D

A

E

A

D

K

F

D

N

N

V

V

E

T

T

L

D

K

Y

Y

R

S

N

N

P

D

P

P

N

-

G

-

D

D

L

S

A

T

D

K

M

E

S

A

R

V

L

L

I

I

E

Q

Q

D

A

A

A

V

A

N

R

A

N

K

Y

V

D

D

G

G

V

I

I

A

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T

I

I

A

P

D

D

F

P

D

P

V

A

L

L

K

I

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P

S

A

I

I

E

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K

Q

V

A

T

V

A

A

K

A

K

G

I

I

A

P

L

V

V

V

T

A

I

F

N
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N

S

A

G

G

T

I

N

-

E

D

Q

Q

S

W

A

K

Q

E

L

S

A

G

A

A

I

L

M

M

H

Y

I

F

G

G

Q

Q

P

D

E

E

Y

T

L

V

A

A

G

G

K

Q

A

A

G

G

E

A

K

R

A

A

K

T

A

S

A

E

G

G

V

F

K

K

T

H

F

V

L

L

C

C

V

V

N

L

H
x
Q

F

-

A

A

T

Q

N

G

P

Q

V

V

S
x
Q

F

L

D

E

-

S

R
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R

C

C

R

N

G

G

F

V

A

Q

D

Q

A

T

I

F

G

K

A

G

D

Q

Y

Y

K

-

T

T

S

K

T

L

I

Y

D

V

S

N

G

G

Q

A

D

D

P

Q

T

P

E

S

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T

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S

A

A

A

Y

K

L

L

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N

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P

K

S

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I

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D

A

L

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V

L

I

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T

L
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L

G

G

P

A

P

P

P

I

A

A

S

Q

A

L

T

A

L

I

K

Q

A

A

I

V

D

K

Q

D

M

A

G

G

I

S

A

N

G

A

K

K

I

I

F

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C

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F

F

D
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D

F

F

S

N

D

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E

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I

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A

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K

A

A

E

I

I

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E

D

N

G

G

T

Q

I

L

A

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F

W

A

A

I

I

D

D

Q
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Q

Q

Q

P

P

Y

Y

L

V

Q

E

G

G

Y

Y

M

E

A

A

V

V

A

D

V

S

L

L

A

-

I

-

A

-

K

-

E

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R

-

T

-

D

-

P

-

V

-

K

-

I

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R

-

Q

-

V

-

L

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K

-

E

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N

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V

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K

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F

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K

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A

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R

W

L

L

E

Y

M

I

Y

T

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L

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E

D

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I

Y

G

G

G

P

G

K

E

N

A

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K

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T

G

G

P

P

S

F

F

F

I

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T

D

K

K

A

S

N

N

L

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A

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V

V

A

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K

Y

F

A

A

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H12 (= H44), D17 (= D49), W20 (= W52), N92 (= N126), Q135 (≠ H170), Q141 (≠ S177), R145 (= R180), L190 (= L226), D215 (= D253), Q235 (= Q273)

6guqA Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with glucose
29% identity, 71% coverage: 38:287/351 of query aligns to 3:252/278 of 6guqA

query
sites
6guqA

H

H

F

F

V

V

L

L

V

V

S

P

H
x
E

A

E

P

L

D

D

S
x
N

D

D

T

-

F
x
Y

W

W

N

R

T

L

I

V

R

E

N

K

A

G

I

A

E

K

Q

A

A

A

D

A

E

K

D

E

F

L

N

G

V

V

E

D

T

L

D

E

Y

Y

R

I

N

G

P

P

P

R

N

Q

G

A

D

N

L

I

A

D

D

E

M

H

S

L

R

R

L

I

I

L

E

K

Q

K

A

A

R

A

N

K

Y

V

D

D

G

G

V

I

I

I

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T

-

Q

-

G

-

L

T

T

I

E

A

A

D

E

F

F

D

-

V

-

L

-

K

V

S

P

S

V

I

I

E

N

K

E

V

I

T

T

A

D

K

K

K

N

I

I

A

P

L

V

V

V

T

T

I

I

N
x
D

S

-

G

-

T

T

N

D

E

A

Q

P

S

T

A

S

Q

R

L

R

A

V

A

A

I

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M

-

H

Y

I

V

G

G

Q

T

P

D

E

N

Y

Y

-

Y

-

A

-

G

-

F

L

L

A

A

G

G

K

R

A

A

A

L

G

A

E

E

K

D

A

T

K

K

A

-

G

G

V

K

K

A

T

T

F

V

L

A

C

I

V

I

N

T

H

G

F

S

A

L

T

T

N

A

P

A

V
x
H

S

Q

F

Q

D

L

R
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R

C

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R

R

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G

F

F

A

E

D

D

A

A

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R

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Y

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I

S

R

T

I

I

V

D

A

S

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G

E

Q

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D

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H

-
x
I

T

T

E

R

I

V

Q

Q

S

A

K

A

V

E

S

K

A

A

Y

Y

-

T

-

I

L

L

R

K

N

K

H

H

P

P

K

D

T

V

D

N

A

A

V

F

L

Y

T

G

L

T

G
x
S

P
x
A

P

L

P

D

A

A

S

I

A

G

T

V

L

A

K

K

A

V

I

V

D

E

Q

Q

M

F

G

H

I

R

A

E

G

Q

K

K

I

T

F

Y

F

I

C

I

T

G

F

F

D
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D

F

T

S

L

D

P

E

E

I

T

A

I

K

R

A

Y

I

L

R

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D

K

G

G

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I

I

A

A

F

A

A

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Q
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Q

Q

E

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P

Y

Y

L

E

Q

M

G

G

Y

Y

M

K

A

A

V

V

A

K

V

M

L

M

A

A

binding beta-D-glucopyranose: E9 (≠ H44), N13 (≠ S48), Y15 (≠ F51), D90 (≠ N126), H138 (≠ V176), R142 (= R180), I166 (vs. gap), S192 (≠ G227), A193 (≠ P228), D218 (= D253), Q238 (= Q273)

6gt9A Crystal structure of ganp, a glucose-galactose binding protein from geobacillus stearothermophilus, in complex with galactose
29% identity, 71% coverage: 38:287/351 of query aligns to 8:257/283 of 6gt9A

query
sites
6gt9A

H

H

F

F

V

V

L

L

V

V

S

P

H
x
E

A

E

P

L

D

D

S
x
N

D

D

T

-

F
x
Y

W
|
W

N

R

T

L

I

V

R

E

N

K

A

G

I

A

E

K

Q

A

A

A

D

A

E

K

D

E

F

L

N

G

V

V

E

D

T

L

D

E

Y

Y

R

I

N

G

P

P

P

R

N

Q

G

A

D

N

L

I

A

D

D

E

M

H

S

L

R

R

L

I

I

L

E

K

Q

K

A

A

R

A

N

K

Y

V

D

D

G

G

V

I

I

I

T

T

-

Q

-

G

-

L

T

T

I

E

A

A

D

E

F

F

D

-

V

-

L

-

K

V

S

P

S

V

I

I

E

N

K

E

V

I

T

T

A

D

K

K

K

N

I

I

A

P

L

V

V

V

T

T

I

I

N
x
D

S

-

G

-

T

T

N

D

E

A

Q

P

S

T

A

S

Q

R

L

R

A

V

A

A

I

-

M

-

H

Y

I

V

G

G

Q

T

P

D

E

N

Y

Y

-

Y

-

A

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G

-

F

L

L

A

A

G

G

K

R

A

A

A

L

G

A

E

E

K

D

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K

K

A

-

G

G

V

K

K

A

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L

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C

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H

G

F

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L

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N

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A

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x
H

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D

L

R
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R

C

V

R

R

G

G

F

F

A

E

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D

A

A

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R

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D

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K

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G

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Q

E

D

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H

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x
I

T

T

E

R

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Q

Q

S

A

K

A

V

E

S

K

A

A

Y

Y

-

T

-

I

L

L

R

K

N

K

H

H

P

P

K

D

T

V

D

N

A

A

V

F

L

Y

T

G

L

T

G
x
S

P
x
A

P

L

P

D

A

A

S

I

A

G

T

V

L

A

K

K

A

V

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D

E

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Q

M

F

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H

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R

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E

G

Q

K

K

I

T

F

Y

F

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C

I

T

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F

F

D
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D

F

T

S

L

D

P

E

E

I

T

A

I

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R

A

Y

I

L

R

Q

D

K

G

G

T

T

I

I

A

A

F

A

A

T

I

V

D

V

Q
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Q

Q

E

P

P

Y

Y

L

E

Q

M

G

G

Y

Y

M

K

A

A

V

V

A

K

V

M

L

M

A

A

binding beta-D-galactopyranose: E14 (≠ H44), N18 (≠ S48), Y20 (≠ F51), W21 (= W52), D95 (≠ N126), H143 (≠ V176), R147 (= R180), I171 (vs. gap), S197 (≠ G227), A198 (≠ P228), D223 (= D253), Q243 (= Q273)

2h3hA Crystal structure of the liganded form of thermotoga maritima glucose binding protein (see paper)
24% identity, 84% coverage: 51:345/351 of query aligns to 14:294/313 of 2h3hA

query
sites
2h3hA

F

Y

W

W

N

S

T

Q

I

V

R

E

N

Q

A

G

I

V

E

K

Q

A

A

A

D

G

E

K

D

A

F

L

N

G

V

V

E

D

T

T

D

K

Y

F

R

F

N

V

P

P

P

Q

N

K

G

E

D

D

L

I

A

N

D

A

M

Q

S

L

R

Q

L

M

I

L

E

E

Q

S

A

F

A

I

A

A

R

E

N

G

Y

V

D

N

G

G

V

I

I

A

T

I

T

A

I

P

A

S

D

D

F

P

D

T

V

A

L

V

K

I

S

P

S

T

I

I

E

K

K

K

V

A

T

L

A

E

K

M

K

G

I

I

A

P

L

V

V

V

T

T

I

L

N
x
D

S

T

G

-

T

-

N

-

E

-

Q

D

S

S

A

P

Q

D

L

S

A

G

A

R

I

Y

M

V

H

Y

I

I

G

G

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P

D

E

N

Y

Y

L

Q

A

A

G

G

K

Y

A

T

A

A

G

G

-

L

-

I

E

M

K

K

A

E

K

L

A

L

A

G

G

G

V

K

K

G

T

K

F

V

L

V

C

I

V

G

N

T

H

G

F

S

A

L

T

T

N

A

P

M

V
x
N

S

S

F

L

D

Q

R
|
R

C

I

R

Q

G

G

F

F

A

K

D

D

A

A

I

I

G

-

A

K

D

D

Y

S

K

E

T

I

S

E

T

I

I

V

D

D

S

I

G

L

Q

N

D

D

P

E

T
x
E

E

D

I

G

Q

A

S

R

K

A

V

V

S

S

-

L

-

A

-

E

A

A

Y

A

L

L

R

N

N

A

H

H

P

P

K

D

T

L

D

D

A

A

V

F

L

F

T

G

L

V

G
x
Y

P
x
A

P

Y

P

N

A

G

S

P

A

A

T

Q

L

A

K

L

A

V

I

V

D

K

Q

N

M

A

G

G

I

K

A

V

G

G

K

K

I

V

F

K

F

I

C

V

T

C

F

F

D
|
D

F

T

S

T

D

P

E

D

I

I

A

L

K

Q

A

Y

I

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R

K

D

E

G

G

T

V

I

I

A

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F

A

A

T

I

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D

G

Q
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Q

Q

R

P

P

Y

Y

L

M

Q

M

G

G

Y

Y

M

L

A

S

V

V

A

T

V

V

L

L

A

Y

I

L

A

M

K

N

K

K

E

I

R

G

T

V

T

Q

D

N

P

T

V

L

K

-

I

-

R

-

Q

-

V

M

L

M

K

L

E

P

N

K

V

V

K

K

F

V

K

D

A

G

R

K

L

V

E

D

M

Y

Y

V

G

-

L

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E

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P

-

S

-

Y

-

G

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P

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K

-

N

-

I

I

R

D

S

T

G

G

P

V

S

D

F

V

I

V

T

T

K

P

A

E

N

N

L

L

D

D

K

E

V

Y

V

L

K

K

binding beta-D-glucopyranose: D89 (≠ N126), N137 (≠ V176), R141 (= R180), E164 (≠ T204), Y190 (≠ G227), A191 (≠ P228), D216 (= D253), Q236 (= Q273)

Sites not aligning to the query:

binding beta-D-glucopyranose: 9

3c6qC Apo and ligand-bound form of a thermophilic glucose/xylose binding protein
23% identity, 84% coverage: 51:345/351 of query aligns to 14:294/305 of 3c6qC

query
sites
3c6qC

F

Y

W
|
W

N

S

T

Q

I

V

R

E

N

Q

A

G

I

V

E

K

Q

A

A

A

D

G

E

K

D

A

F

L

N

G

V

V

E

D

T

T

D

K

Y

F

R

F

N

V

P

P

P

Q

N

K

G

C

D

D

L

I

A

N

D

A

M

Q

S

L

R

Q

L

M

I

L

E

E

Q

S

A

F

A

I

A

A

R

E

N

G

Y

V

D

N

G

G

V

I

I

A

T

I

T

A

I

P

A

S

D

D

F

P

D

T

V

A

L

V

K

I

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P

S

T

I

I

E

K

K

K

V

A

T

L

A

E

K

M

K

G

I

I

A

P

L

V

V

V

T

T

I

L

N
x
D

S

T

G

-

T

-

N

-

E

-

Q

D

S

S

A

P

Q

D

L

S

A

G

A

R

I

Y

M

V

H

Y

I

I

G

G

Q

T

P

D

E

N

Y

Y

L

Q

A

A

G

G

K

Y

A

T

A

A

G

G

-

L

-

I

E

M

K

K

A

E

K

L

A

L

A

G

G

G

V

K

K

G

T

K

F

V

L

V

C

I

V

G

N

T

H

G

F

S

A

L

T

T

N

A

P

M

V
x
N

S

S

F

L

D

Q

R
|
R

C

I

R

Q

G

G

F

F

A

K

D

D

A

A

I

I

G

-

A

K

D

D

Y

S

K

E

T

I

S

E

T

I

I

V

D

D

-

I

-

L

-

N

S

D

G

C

Q
x
E

D

D

P

G

T

A

E

R

I

A

Q

V

S

S

K

L

V

A

S

E

A

A

Y

A

L

L

R

N

N

A

H

H

P

P

K

D

T

L

D

D

A

A

V

F

L

F

T

G

L

V

G
x
Y

P
x
A

P

Y

P

N

A

G

S

P

A

A

T

Q

L

A

K

L

A

V

I

V

D

K

Q

N

M

A

G

G

I

K

A

V

G

G

K

K

I

V

F

K

F

I

C

V

T

C

F

F

D
|
D

F

T

S

T

D

P

E

D

I

I

A

L

K

Q

A

Y

I

V

R

K

D

E

G

G

T

V

I

I

A

Q

F

A

A

T

I

M

D

G

Q
|
Q

Q

R

P

P

Y

Y

L

M

Q

M

G

G

Y

Y

M

L

A

S

V

V

A

T

V

V

L

L

A

Y

I

L

A

M

K

N

K

K

E

I

R

G

T

V

T

Q

D

N

P

T

V

L

K

-

I

-

R

-

Q

-

V

M

L

M

K

L

E

P

N

K

V

V

K

K

F

V

K

D

A

G

R

K

L

V

E

D

M

Y

Y

V

G

-

L

-

E

-

P

-

S

-

Y

-

G

-

P

-

K

-

N

-

I

I

R

D

S

T

G

G

P

V

S

D

F

V

I

V

T

T

K

P

A

E

N

N

L

L

D

D

K

E

V

Y

V

L

K

K

binding beta-D-xylopyranose: W15 (= W52), D89 (≠ N126), N137 (≠ V176), R141 (= R180), E164 (≠ Q201), Y190 (≠ G227), A191 (≠ P228), D216 (= D253), Q236 (= Q273)

Sites not aligning to the query:

binding beta-D-xylopyranose: 9

2ioyA Crystal structure of thermoanaerobacter tengcongensis ribose binding protein (see paper)
27% identity, 75% coverage: 48:309/351 of query aligns to 11:274/274 of 2ioyA

query
sites
2ioyA

S

N

D
x
N

T

P

F
|
F

W
x
F

N

V

T

T

I

L

R

K

N

N

A

G

I

A

E

E

Q

E

A

K

D

A

E

K

D

E

F

L

N

G

-

Y

-

K

-

I

V

I

E

V

T

E

D

D

Y

S

R

Q

N

N

P

D

P

S

N

S

G

K

D

E

L

L

A

S

D

N

M

V

S

E

R

D

L

L

I

I

E

Q

Q

Q

A

-

R

-

N

K

Y

V

D

D

G

V

V

L

I

L

T

I

T

N

I

P

A

V

D

D

F

S

D

D

V

A

L

V

K

V

S

T

S

A

I

I

E

K

K

E

V

A

T

N

A

S

K

K

K

N

I

I

A

P

L

V

V

I

T

T

I

I

N
x
D

S
x
R

G

S

T

A

N

N

E

G

Q

G

S

D

A

V

Q

-

L

-

A

-

I

V

M

C

H

H

I

I

G

A

Q

S

P

D

E

N

Y

V

L

K

A

G

G

G

K

E

A

M

A

A

G

A

E

E

-

F

K

I

A

A

K

K

A

A

-

L

A

K

G

G

V

K

K

G

T

N

F

V

L

V

C

E

V

L

N

E

H

G

F

I

A

P

T

G

N

A

P

S

V
x
A

S

A

F

R

D

D

R
|
R

C

G

R

K

G

G

F

F

A

D

D

E

A

A

I

I

G

-

A

A

D

K

Y

Y

K

P

T

D

S

I

T

K

I

I

D

V

S

A

G

K

Q

Q

-

A

-

D

-
x
F

D

D

P

R

T

S

E

K

I

G

Q

L

S

S

K

V

V

M

S

E

A

N

Y

I

L

L

R

Q

N

A

H

Q

P

P

K

K

T

I

D

D

A

A

V

V

L

F

T

A

L

Q

G
x
N

P

D

P

E

P

M

A

A

S

L

A

G

T

A

L

I

K

K

A

A

I

I

D

E

Q

A

M

A

G

N

I

R

A

Q

G

G

K

-

I

I

F

I

F

V

C

V

T

G

F

F

D
|
D

F

G

S

T

D

E

E

D

I

A

A

L

K

K

A

A

I

I

R

K

D

E

G

G

T

K

I

M

A

A

F

A

A

T

I

I

D

A

Q
|
Q

Q

Q

P

P

Y

A

L

L

Q

M

G

G

Y

S

M

L

A

G

V

V

A

E

V

M

L

A

-

D

A

K

I

Y

A

L

K

K

K

G

E

E

R

K

T

I

T

P

D

N

-

F

-

I

P

P

V

A

K

E

I

L

R

K

Q

L

V

I

L

T

K

K

E

E

N

N

V

V

K

Q

binding beta-D-ribopyranose: N12 (≠ D49), F14 (= F51), F15 (≠ W52), D88 (≠ N126), R89 (≠ S127), A136 (≠ V176), R140 (= R180), F164 (vs. gap), N190 (≠ G227), D215 (= D253), Q235 (= Q273)

5xssA Xylfii molecule (see paper)
22% identity, 71% coverage: 38:286/351 of query aligns to 5:253/274 of 5xssA

query
sites
5xssA

H

K

F

I

V

V

L

L

V

I

S

S

H
|
H

A

I

P

K

D

-

S

T

D

N

T

P

F

Y

W
|
W

N

L

T

D

I

I

R

K

N

A

A

G

I

A

E

E

Q

R

A

A

D

A

E

K

D

E

F

R

N

G

V

A

E

V

T

V

D

E

Y

F

R

L

N

G

P

P

P

T

N

T

G

A

D

S

L

T

A

E

D

D

M

G

S

L

R

K

L

L

I

F

E

D

Q

M

A

A

A

T

A

S

R

A

N

K

Y

V

D

S

G

G

V

I

I

I

T

T

T

Y

I

V

A

Q

D

E

F

E

D

G

V

Q

L

Y

K

K

S

K

I

I

E

N

K

S

V

A

T

M

A

E

K

K

K

G

I

I

A

P

L

V

V

V

T

T

I

I

N
x
D

S

S

G

-

T

D

N

E

E

E

Q

D

S

S

A

N

Q

R

L

I

A

A

A

-

I

-

M

-

H

Y

I

V

G

G

Q

T

P

D

E

N

Y

V

L

L

A

A

G

G

K

Q

A

V

A

A

G

G

-

K

E

E

K

M

A

V

K

K

A

Q

A

I

G

G

V

T

K

S

T

G

F

N

L

V

C

A

V

I

N

V

H

M

F

G

A

G

T

K

N

N

P

V

V

K

S

N

-

Q

F

K

D

E

R
|
R

C

V

R

E

G

G

F

F

A

T

D

Q

A

Y

I

I

G

K

A

S

D

N

Y

S

K

N

T

L

S

K

T

I

I

V

D

D

S

T

-

D

G

S

Q

S

D

D

P

A

T

M

E

L

I

L

Q

E

S

A

K

E

V

I

-

I

-

T

S

R

A

K

Y

I

L

L

R

N

N

R

H

N

P

D

K

N

T

I

D

N

A

A

V

L

L

F

T

C

L

T

G
x
S

P
x
A

P

L

P

D

A

G

S

I

A

G

T

A

L

A

K

R

A

A

I

V

D

K

Q

D

M

L

G

N

I

Y

A

K

G

D

K

R

I

V

F

K

F

I

C

I

T

C

F

F

D
|
D

F

D

S

L

D

D

E

D

I

T

A

L

K

S

A

N

I

I

R

R

D

N

G

G

T

L

I

V

A

S

F

A

A

T

I

I

D

V

Q
|
Q

Q

K

P

S

Y

N

L

E

Q

M

G

G

Y

Y

M

R

A

A

V

V

A

N

V

I

L

I

binding beta-D-xylopyranose: H11 (= H44), W18 (= W52), D92 (≠ N126), R144 (= R180), S194 (≠ G227), A195 (≠ P228), D220 (= D253), Q240 (= Q273)

4wutA Crystal structure of an abc transporter solute binding protein (ipr025997) from agrobacterium vitis (avi_5133, target efi-511220) with bound d-fucose
23% identity, 70% coverage: 48:294/351 of query aligns to 11:264/290 of 4wutA

query
sites
4wutA

S

N

D

S

T

T

F

F

W
|
W

N

Q

T

N

I

V

R

Q

N

K

A

G

I

A

E

D

Q

A

A

A

D

I

E

G

D

K

F

Q

N

K

V

A

E

H

T

T

D

I

Y

T

R

F

N

Q

P

G

P

P

N

A

G

A

D
x
E

-

S

-

A

L

I

A

A

D

D

M

Q

S

V

R

N

L

M

I

V

E

E

Q

N

A

A

A

V

A

N

R

R

N

K

Y

V

D

D

G

A

V

I

I

L

T

L

T

A

I

P

A

S

D

D

F

P

D

D

V

A

L

L

K

V

S

P

S

A

I

V

E

K

K

K

V

A

T

W

A

E

K

A

K

R

I

I

A

P

L

V

V

V

T

I

I

I

N
x
D

S

S

G

M

T

L

N

S

E

K

Q

D

S

A

A

E

Q

K

L

Y

A

-

I

-

M

-

H

-

I

-

G

-

Q

Y

P

Q

E

A

Y

F

L

L

A

A

-

T

-

D

G

N

K

K

A

A

G

G

E

E

K

L

A

A

K

A

A

K

A

A

G

M

V

I

K

Q

T

K

-

V

-

G

-

T

-

E

-

G

-

K

F

I

L

A

C

V

V

M

N

S

H

Y

F

V

A

A

T

G

N

A

P

G

V

S

S

E

F

I

D

G

R
|
R

C

V

R

G

G

G

F

F

A

T

D

D

A

Y

I

I

G
x
K

A

A

D

N

Y
x
S

K

K

T

L

S

Q

T

I

I

V

D

G

S

-

G

-

Q

-

D

-

P

P

T

Y

E

Y

I

S

Q

Q

S

S

K

Q

V

M

S

A

A

T

Y

A

L

L

R
x
N

N

Q

-

T

-
x
D

-

V

-

L

-

A

H

N

P

A

K

D

T

L

D

K

A

G

V

I

L

F

T

G

L

A

G
x
N

P

E

P

P

P

T

A

A

S

I

A

G

T

M

L

G

K

R

A

A

I

I

D

K

Q

Q

M

A

G

G

I

K

A

A

G

G

K

K

I

L

F

V

F

A

C

I

T

G

F

F

D
|
D

F

G

S

N

D

Q

E

D

I

L

A

Q

K

E

A

F

I

V

R

K

D

D

G

G

T

T

I

L

A

E

F

A

A

T

I

V

D

V

Q
|
Q

Q

G

P

S

Y

Y

L

Q

Q

M

G

G

Y

E

M

K

A

G

V

V

-

D

A

T

V

L

L

L

A

K

I

L

A

L

K

S

K

K

E

E

R

K

T

V

binding calcium ion: K155 (≠ G189), S158 (≠ Y192), N177 (≠ R215), D181 (vs. gap)
binding beta-D-fucopyranose: W15 (= W52), E42 (≠ D79), D91 (≠ N126), R146 (= R180), N196 (≠ G227), D222 (= D253), Q242 (= Q273)

Sites not aligning to the query:

binding beta-D-fucopyranose: 8

4rxmA Crystal structure of periplasmic abc transporter solute binding protein a7jw62 from mannheimia haemolytica phl213, target efi-511105, in complex with myo-inositol
22% identity, 54% coverage: 87:275/351 of query aligns to 53:245/291 of 4rxmA

query
sites
4rxmA

I

L

E

E

Q

N

A

A

A

I

A

T

R

R

N

G

Y

A

D

K

G

G

V

I

I

I

T

I

T

S

I

P

A

N

D

D

F

V

D

N

V

A

L

I

K

S

S

G

S

A

I

V

E

E

K

E

V

I

T

I

A

K

K

E

K

K

I

I

A

P

L

A

V

A

T

T

I

L

N
x
D

S
x
R

G

K

T

V

N

E

E

S

Q

S

S

K

A

P

Q

-

L

-

A

-

I

V

M

P

H

H

I

F

G

G

Q

A

P

N

E

N

Y

Y

L

T

A

G

G

G

K

Q

A

E

A

V

G

A

E

K

K

A

A

V

K

K

A

A

A

K

-

Y

-

P

-

N

G

G

V

A

K

K

T

I

F

I

L

L

C

L

V

T

N

G

H

Q

F

P

A

G

T

S

N

T

P
x
S

V

-

S

N

F

I

D

E

R
|
R

C

T

R

K

G

G

F

I

A

R

D

D

A

E

I

L

-

A

-

G

G

G

A

D

D

K

Y

Y

K

K

T

I

S

V

T

V

I

D

D

Q

S

T

G

G

Q

N

-
x
W

-

L

-

R

-

S

D

E

P

G

T

L

E

R

I

I

Q

I

S

E

K

S

V

V

S

L

A

P

Y

T

L

L

R

K

N

E

H

K

P

P

K

-

T

-

D

E

A

V

V

I

L

I

T

S

L

A

G
x
N

P

D

P

M

A

A

S

L

A

G

T

A

L

I

K

E

A

A

I

L

D

R

Q

S

M

Q

G

G

I

L

-

K

A

A

G

G

K

D

I

I

F

L

F

V

C

T

T

G

F

F

D
|
D

F

S

S

T

D

P

E

E

I

A

A

L

K

A

A

R

I

V

R

K

D

D

G

G

T

W

I

L

A

Y

F

L

A

T

I

A

D

D

Q
|
Q

Q

R

P

P

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: D92 (≠ N126), R93 (≠ S127), S140 (≠ P175), R144 (= R180), W169 (vs. gap), N196 (≠ G227), D223 (= D253), Q243 (= Q273)

Sites not aligning to the query:

4rxmB Crystal structure of periplasmic abc transporter solute binding protein a7jw62 from mannheimia haemolytica phl213, target efi-511105, in complex with myo-inositol
22% identity, 54% coverage: 87:275/351 of query aligns to 51:243/288 of 4rxmB

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binding aspartic acid: L183 (= L214), K184 (≠ R215), E185 (≠ N216)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: D90 (≠ N126), R91 (≠ S127), S138 (≠ P175), R142 (= R180), W167 (vs. gap), N194 (≠ G227), D221 (= D253), Q241 (= Q273)

Sites not aligning to the query:

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 17

3ksmA Crystal structure of abc-type sugar transport system, periplasmic component from hahella chejuensis
29% identity, 32% coverage: 172:283/351 of query aligns to 137:251/276 of 3ksmA

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binding beta-D-ribofuranose: N139 (= N174), S141 (≠ V176), R145 (= R180), N195 (≠ G227), D221 (= D253), Q241 (= Q273)

Sites not aligning to the query:

binding beta-D-ribofuranose: 8, 14, 91, 135

1dbpA Identical mutations at corresponding positions in two homologous proteins with non-identical effects (see paper)
24% identity, 68% coverage: 68:306/351 of query aligns to 35:271/271 of 1dbpA

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binding beta-D-ribopyranose: D89 (≠ N126), R90 (≠ E131), R141 (= R180), F164 (vs. gap), N190 (≠ G227), D215 (= D253)

Sites not aligning to the query:

binding beta-D-ribopyranose: 13, 15

Query Sequence

>BPHYT_RS12000 FitnessBrowser__BFirm:BPHYT_RS12000
MTLGNGKASRASLRVLVAALALTAGFAAQPAARAVDAHFVLVSHAPDSDTFWNTIRNAIE
QADEDFNVETDYRNPPNGDLADMSRLIEQAAARNYDGVITTIADFDVLKSSIEKVTAKKI
ALVTINSGTNEQSAQLAAIMHIGQPEYLAGKAAGEKAKAAGVKTFLCVNHFATNPVSFDR
CRGFADAIGADYKTSTIDSGQDPTEIQSKVSAYLRNHPKTDAVLTLGPPPASATLKAIDQ
MGIAGKIFFCTFDFSDEIAKAIRDGTIAFAIDQQPYLQGYMAVAVLAIAKKERTTDPVKI
RQVLKENVKFKARLEMYGLEPSYGPKNIRSGPSFITKANLDKVVKYAGQYR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory