SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS32970 FitnessBrowser__BFirm:BPHYT_RS32970 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4jbbA Crystal structure of glutathione s-transferase a6tby7(target efi- 507184) from klebsiella pneumoniae mgh 78578, gsh complex
56% identity, 95% coverage: 6:205/210 of query aligns to 4:206/208 of 4jbbA

query
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active site: Y51 (≠ K53), A52 (≠ S54)
binding glutathione: S14 (= S16), Y16 (= Y18), H43 (≠ N45), R56 (= R58), V57 (= V59), E69 (= E71), S70 (= S72)

P77544 Glutathione S-transferase YfcF; EC 2.5.1.18 from Escherichia coli (strain K12) (see paper)
51% identity, 98% coverage: 1:205/210 of query aligns to 1:208/214 of P77544

query
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P77544

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S10 (≠ A10) mutation to A: Nearly no effect on GSH transferase activity.
S16 (= S16) mutation to G: 2% of wild-type GSH transferase activity.

3bbyA Crystal structure of glutathione s-transferase (np_416804.1) from escherichia coli k12 at 1.85 a resolution
50% identity, 96% coverage: 5:205/210 of query aligns to 3:188/191 of 3bbyA

query
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3bbyA

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binding calcium ion: D75 (= D92), E77 (≠ H94)

4isdA Crystal structure of glutathione transferase homolog from burkholderia gl bgr1, target efi-501803, with bound glutathione
50% identity, 96% coverage: 6:207/210 of query aligns to 5:185/187 of 4isdA

query
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4isdA

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binding glutathione: Q43 (≠ A44), R51 (= R58), V52 (= V59), E64 (= E71), S65 (= S72)

2v6kA Structure of maleyl pyruvate isomerase, a bacterial glutathione-s- transferase in zeta class, in complex with substrate analogue dicarboxyethyl glutathione (see paper)
35% identity, 46% coverage: 24:120/210 of query aligns to 19:115/214 of 2v6kA

query
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2v6kA

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binding acetate ion: R111 (≠ E116), E115 (= E120)
binding gamma-glutamyl-s-(1,2-dicarboxyethyl)cysteinylglycine: H40 (≠ N45), L53 (≠ R58), V54 (= V59), Q66 (≠ E71), S67 (= S72), H106 (≠ M111), N110 (≠ Q115), R111 (≠ E116), R112 (= R117)

Sites not aligning to the query:

binding gamma-glutamyl-s-(1,2-dicarboxyethyl)cysteinylglycine: 11, 12, 13

2jl4A Holo structure of maleyl pyruvate isomerase, a bacterial glutathione- s-transferase in zeta class (see paper)
35% identity, 46% coverage: 24:120/210 of query aligns to 17:113/212 of 2jl4A

query
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2jl4A

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binding glutathione: H38 (≠ N45), L51 (≠ R58), V52 (= V59), Q64 (≠ E71), S65 (= S72), H104 (≠ M111), N108 (≠ Q115), R109 (≠ E116), R110 (= R117)

Sites not aligning to the query:

binding glutathione: 9, 11

O86043 Maleylpyruvate isomerase; MPI; Naphthalene degradation protein L; EC 5.2.1.4 from Ralstonia sp. (see paper)
35% identity, 46% coverage: 24:120/210 of query aligns to 17:113/212 of O86043

query
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O86043

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H38 (≠ N45) binding
V52 (= V59) binding
QS 64:65 (≠ ES 71:72) binding
DIH 102:104 (≠ DLM 109:111) binding
NRR 108:110 (≠ QER 115:117) binding

Sites not aligning to the query:

9:11 binding
176 binding

Q64471 Glutathione S-transferase theta-1; GST class-theta-1; EC 2.5.1.18 from Mus musculus (Mouse) (see paper)
30% identity, 63% coverage: 6:138/210 of query aligns to 3:138/240 of Q64471

query
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Q64471

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S

E

D

S

A

Y

F

A

A

A

R

K

V

S

N

L

P

T

M

Q

K

R

R

V

V

P

P

T

A

L

M

T

M

H

D

G

G

A

G

F

F

A

T

L

L

S

C

E

E

S

S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

D

A

D

H

T

K

F

Y

-

K

A

V

E

P

T

D

L

H

V

W

Y

Y

P

P

Q

Q

D

D

R

L

H

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

L

Y

Q

L

A

A

W

W

-

Q

-

H

-

T

-

G

L

L

R

R

S

R

D

S

L

C

M

L

P

R

I

A

R

L

Q

W

E

H

R

K

S

V

T

M

E

F

V

P

V

V

F

F

Y

L

K

G

R

E

Q

Q

A

I

P

P

F

E

S

T

A

L

K

A

A

A

Q

T

L

L

A

A

Sites not aligning to the query:

234 R→W: Strongly reduced catalytic activity.

P30711 Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1; EC 2.5.1.18 from Homo sapiens (Human) (see 2 papers)
34% identity, 50% coverage: 5:110/210 of query aligns to 2:106/240 of P30711

query
sites
P30711

S

G

L

L

R

E

L

L

Y

Y

A

L

D

D

A

-

Q

-

F

L

A

L

S

S

P

Q

Y

P

A

C

M

R

S

A

V

V

F

Y

V

I

A

F

L

A

Q

K

E

K

K

N

Q

D

L

I

P

P

F

F

E

E

L

L

F

R

A

I

V

V

D

D

L

L

G

I

T

K

D

G

A

Q

N

H

H

L

E

S

E

D

S

A

Y

F

A

A

A

Q

K

V

S

N

L

P

T

L

Q

K

R

K

V

V

P

P

T

A

L

L

T

K

H

D

G

G

A

D

F

F

A

T

L

L

S

T

E

E

S

S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

D

T

D

R

T

K

F

Y

-

K

A

V

E

P

T

D

L

Y

V

W

Y

Y

P

P

Q

Q

D

D

R

L

H

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

L

Y

Q

L

A

A

W

W

L

Q

R

H

S

T

D

T

L

L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
169 V → I: in dbSNP:rs2266637
176 H→Q: Increases activity towards alkylhalogenides, but not hydroperoxides.
234 W→R: Facilitates binding of substrates and increases catalytic activity.

2c3qA Human glutathione-s-transferase t1-1 w234r mutant, complex with s- hexylglutathione (see paper)
34% identity, 50% coverage: 5:110/210 of query aligns to 1:105/239 of 2c3qA

query
sites
2c3qA

S

G

L

L

R

E

L

L

Y

Y

A

L

D

D

A

-

Q

-

F

L

A

L

S
|
S

P

Q

Y

P

A

C

M

R

S

A

V

V

F

Y

V

I

A

F

L

A

Q

K

E

K

K

N

Q

D

L

I

P

P

F

F

E

E

L

L

F

R

A

I

V

V

D

D

L

L

G

I

T

K

D

G

A

Q

N
x
H

H

L

E

S

E

D

S

A

Y

F

A

A

A

Q

K

V

S

N

L

P

T

L

Q

K

R
x
K

V
|
V

P

P

T

A

L

L

T

K

H

D

G

G

A

D

F

F

A

T

L

L

S

T

E
|
E

S
|
S

S

V

A

A

I

I

T

L

E

L

Y

Y

L

L

D

T

D

R

T

K

F

Y

-

K

A

V

E

P

T

D

L

Y

V

W

Y

Y

P

P

Q

Q

D

D

R

L

H

Q

L

A

R

R

A

A

R

R

A

V

R

D

Q

E

L

Y

Q

L

A

A

W

W

L

Q

R

H

S

T

D

T

L

L

active site: S10 (= S16)
binding s-hexylglutathione: S10 (= S16), H39 (≠ N45), K52 (≠ R58), V53 (= V59), E65 (= E71), S66 (= S72)

Sites not aligning to the query:

binding s-hexylglutathione: 114, 233, 237

7zvpA Crystal structure of poplar glutathione transferase u19 in complex with glutathione (see paper)
32% identity, 41% coverage: 14:100/210 of query aligns to 9:93/216 of 7zvpA

query
sites
7zvpA

F

W

A

A

S
|
S

P

P

Y
x
F

A

S

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

Y

F

I

A

E

V

E

D

D

L

L

G

A

T

N

D
x
K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R
x
Q

V
x
I

P

P

T

V

L

F

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E
|
E

S
|
S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding S-Hydroxy-Glutathione: S11 (= S16), F13 (≠ Y18), K38 (≠ D43), Q51 (≠ R58), I52 (≠ V59), E64 (= E71), S65 (= S72)

8a0rA Crystal structure of poplar glutathione transferase u20 in complex with pinocembrin (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 6:92/215 of 8a0rA

query
sites
8a0rA

A

G

Q

S

F

W

A

V

S

S

P
|
P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L

L

G

T

T

N

D

K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R

K

V

I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E

E

S

S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding pinocembrin: P11 (= P17), F12 (≠ Y18)

Sites not aligning to the query:

binding pinocembrin: 105, 155, 163

8a0qA Crystal structure of poplar glutathione transferase u20 in complex with baicalein (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 6:92/215 of 8a0qA

query
sites
8a0qA

A

G

Q

S

F

W

A

V

S

S

P
|
P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L

L

G

T

T

N

D

K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R

K

V

I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E

E

S

S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: P11 (= P17), F12 (≠ Y18)

Sites not aligning to the query:

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: 105, 155, 159, 163

8a0oA Crystal structure of poplar glutathione transferase u20 in complex with galangin (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 6:92/215 of 8a0oA

query
sites
8a0oA

A

G

Q

S

F

W

A
x
V

S
|
S

P
|
P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L

L

G

T

T

N

D

K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R

K

V

I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E

E

S

S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding galangin: V9 (≠ A15), S10 (= S16), P11 (= P17), F12 (≠ Y18)

Sites not aligning to the query:

binding galangin: 102, 155, 160, 163, 206

8a0iA Crystal structure of poplar glutathione transferase u20 in complex with glutathionylphenylacetophenone (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 6:92/215 of 8a0iA

query
sites
8a0iA

A

G

Q

S

F

W

A

V

S
|
S

P

P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L
|
L

G

T

T

N

D

K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R
x
K

V
x
I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E
|
E

S
|
S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding L-gamma-glutamyl-S-(2-biphenyl-4-yl-2-oxoethyl)-L-cysteinylglycine: S10 (= S16), F12 (≠ Y18), L34 (= L40), K50 (≠ R58), I51 (≠ V59), E63 (= E71), S64 (= S72)

Sites not aligning to the query:

binding L-gamma-glutamyl-S-(2-biphenyl-4-yl-2-oxoethyl)-L-cysteinylglycine: 106, 109, 163, 207, 214

8a08A Crystal structure of poplar glutathione transferase u20 in complex with glutathione (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 6:92/215 of 8a08A

query
sites
8a08A

A

G

Q

S

F

W

A

V

S
|
S

P

P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L

L

G

T

T

N

D
x
K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R
x
K

V
x
I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E
|
E

S
|
S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding glutathione: S10 (= S16), F12 (≠ Y18), K37 (≠ D43), K50 (≠ R58), I51 (≠ V59), E63 (= E71), S64 (= S72)

8a0pA Crystal structure of poplar glutathione transferase u20 in complex with morin (see paper)
31% identity, 42% coverage: 12:100/210 of query aligns to 7:93/216 of 8a0pA

query
sites
8a0pA

A

G

Q

S

F

W

A

V

S
|
S

P
|
P

Y
x
F

A

N

M

Y

S

R

V

V

F

I

V

W

A

A

L

L

Q

K

E

L

K

K

Q

G

L

V

P

E

F

F

E

E

L

H

F

I

A

V

V

E

D

D

L

L

G

T

T

N

D

K

A

S

N

E

H

L

E

L

S

K

Y

Y

A

-

K

N

S

P

L

V

T

Y

Q

K

R

K

V

I

P

P

T

V

L

L

T

V

H

H

G

G

A

G

F

K

A

P

L

I

S

A

E

E

S

S

S

L

A

V

I

I

T

L

E

E

Y

Y

L

I

D

E

T

T

F

W

A

P

E

E

T

N

L

P

V

L

Y

L

P

P

Q

T

D

D

R

P

H

Y

L

E

R

R

A

A

R

M

A

A

R

R

binding 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one: S11 (= S16), P12 (= P17), F13 (≠ Y18)

Sites not aligning to the query:

binding 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one: 106, 156, 164, 207, 208

3qawA Crystal structure of a glutathione-s-transferase from antarctic clam laternula elliptica in a complex with glutathione (see paper)
28% identity, 41% coverage: 15:101/210 of query aligns to 10:96/219 of 3qawA

query
sites
3qawA

A

G

S
|
S

P

P

Y
x
P

A

C

M

W

S

K

V

V

F

L

V

L

A

V

L

L

Q

Q

E

E

K

K

Q

K

L

I

P

D

F

Y

E

D

L

E

F

K

A

I

V

I

D

S

L
x
F

G

S

T

K

D

K

A

E

N
x
H

H
x
K

E

S

E

E

S

E

Y

I

A

L

A

E

K

L

S

N

L

P

T

R

Q

G

R
x
Q

V
|
V

P

P

T

T

L

F

T

T

H

D

G

G

A

D

F

V

A

V

L

V

S

N

E
|
E

S
|
S

S

T

A

A

I

I

T

C

E

M

Y

Y

L

L

D

E

T

K

F

Y

A

P

E

K

T

V

L

P

V

L

Y

F

P

P

Q

S

D

D

R

T

H

T

L

I

R

R

A

A

R

K

A

V

R

Y

Q

Q

active site: S11 (= S16)
binding glutathione: S11 (= S16), P13 (≠ Y18), F35 (≠ L40), H40 (≠ N45), K41 (≠ H46), Q53 (≠ R58), V54 (= V59), E66 (= E71), S67 (= S72)

2cz2A Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from mus musculus (form-1 crystal)
25% identity, 72% coverage: 14:165/210 of query aligns to 9:154/212 of 2cz2A

query
sites
2cz2A

F

F

A

R

S
|
S

P

S

Y
x
C

A

S

M

W

S

R

V

V

F

R

V

I

A

A

L

L

Q

A

E

L

K

K

Q

G

L

I

P

D

F

Y

E

E

L

I

F

V

A

P

V

I

D

N

L
|
L

G

I

T

K

D

D

A

G

N

G

H

Q

E
x
Q

-

F

-

T

E

E

S

E

Y

F

A

Q

A

T

K

L

S

N

L

P

T

M

Q

K

R
x
Q

V
|
V

P

P

T

A

L

L

T

K

H

I

G

D

A

G

F

I

A

T

L

I

S

V

E
x
Q

S
|
S

S

L

A

A

I

I

T

M

E

E

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binding glutathione: S11 (= S16), C13 (≠ Y18), L35 (= L40), Q42 (≠ E47), Q55 (≠ R58), V56 (= V59), Q68 (≠ E71), S69 (= S72), Q108 (≠ M111), N112 (≠ Q115), S114 (≠ R117)

Q9WVL0 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Mus musculus (Mouse)
25% identity, 72% coverage: 14:165/210 of query aligns to 12:157/216 of Q9WVL0

query
sites
Q9WVL0

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Q45 (≠ E47) binding
V59 (= V59) binding
Q111 (≠ M111) binding

Query Sequence

>BPHYT_RS32970 FitnessBrowser__BFirm:BPHYT_RS32970
MFEGSLRLYADAQFASPYAMSVFVALQEKQLPFELFAVDLGTDANHEESYAAKSLTQRVP
TLTHGAFALSESSAITEYLDDTFAETLVYPQDRHLRARARQLQAWLRSDLMPIRQERSTE
VVFYKRQAPPFSAKAQLAVQKLYFAADRLLSNDASNLFGDWCIADTDLALMLNRLVMNGD
DVPAKLASYAAHQWERASVQQWVTLDRPRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory