SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BPHYT_RS33455 FitnessBrowser__BFirm:BPHYT_RS33455 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00437 Protocatechuate 3,4-dioxygenase beta chain; 3,4-PCD; EC 1.13.11.3 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
48% identity, 99% coverage: 2:233/234 of query aligns to 5:237/239 of P00437

query
sites
P00437

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Y109 (= Y106) binding
Y148 (= Y145) binding
H161 (= H158) binding
H163 (= H160) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4whqF Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5 (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:236/236 of 4whqF

query
sites
4whqF

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active site: Y108 (= Y106), Y147 (= Y145), R157 (= R155), H160 (= H158), H162 (= H160)
binding 4-fluorobenzene-1,2-diol: S38 (≠ P36), I39 (≠ L37), P40 (≠ K38), N66 (= N64), G124 (≠ L122), V126 (≠ A124), K193 (≠ Q191), I195 (vs. gap), A196 (≠ G192), V201 (≠ R198)
binding bicarbonate ion: R109 (≠ V107), H110 (= H108)
binding fe (iii) ion: Y108 (= Y106), H160 (= H158), H162 (= H160)

4whrB Anhydride reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 8.5 (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 4whrB

query
sites
4whrB

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active site: Y108 (= Y106), Y147 (= Y145), R157 (= R155), H160 (= H158), H162 (= H160)
binding 4-fluorobenzene-1,2-diol: R7 (≠ S5), P22 (= P20), I28 (≠ V26), S38 (≠ P36), P40 (≠ K38), P40 (≠ K38), Q41 (≠ E39), R231 (≠ N228), E236 (= E233)
binding 4-fluorooxepine-2,7-dione: Y147 (= Y145), W149 (= W147), R157 (= R155), H160 (= H158), H162 (= H160), I191 (= I189)
binding fe (iii) ion: Y108 (= Y106), Y147 (= Y145), H160 (= H158), H162 (= H160)

4whqB Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5 (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 4whqB

query
sites
4whqB

D

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active site: Y108 (= Y106), Y147 (= Y145), R157 (= R155), H160 (= H158), H162 (= H160)
binding 4-fluorobenzene-1,2-diol: R83 (= R81), R109 (≠ V107), H110 (= H108), K111 (= K109), K193 (≠ Q191), I195 (vs. gap), A196 (≠ G192), V201 (≠ R198), D224 (= D221)
binding (6S)-4-fluoro-6-hydroperoxy-6-hydroxycyclohexa-2,4-dien-1-one: Y108 (= Y106), Y147 (= Y145), W149 (= W147), R157 (= R155), H160 (= H158), H162 (= H160), I191 (= I189)
binding fe (iii) ion: Y108 (= Y106), Y147 (= Y145), H160 (= H158), H162 (= H160)

3lxvM Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
48% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 3lxvM

query
sites
3lxvM

D

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R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

binding 4-nitrocatechol: Y24 (= Y22), Y108 (= Y106), H147 (≠ Y145), W149 (= W147), R157 (= R155), H160 (= H158), H162 (= H160), I191 (= I189)
binding fe (iii) ion: Y108 (= Y106), H160 (= H158), H162 (= H160)

3lktM Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
48% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 3lktM

query
sites
3lktM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

N

R

N

N

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y

H

P

P

W

W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R

R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

binding fe (iii) ion: Y108 (= Y106), H160 (= H158), H162 (= H160)

3pckM Structure of protocatechuate 3,4-dioxygenase complexed with 6- hydroxynicotinic acid n-oxide (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pckM

query
sites
3pckM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y
|
Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding fe (iii) ion: Y105 (= Y106), H157 (= H158), H159 (= H160)
binding 6-hydroxyisonicotinic acid n-oxide: Y24 (= Y22), Y105 (= Y106), W146 (= W147), R154 (= R155), H157 (= H158), H159 (= H160), I188 (= I189)

3pcjM Structure of protocatechuate 3,4-dioxygenase complexed with 2- hydroxyisonicotinic acid n-oxide (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pcjM

query
sites
3pcjM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y
|
Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding fe (iii) ion: Y105 (= Y106), H157 (= H158), H159 (= H160)
binding 2-hydroxyisonicotinic acid n-oxide: Y24 (= Y22), Y144 (= Y145), W146 (= W147), R154 (= R155), H157 (= H158), H159 (= H160)

3pciM Structure of protocatechuate 3,4-dioxygenase complexed with 3-iodo-4- hydroxybenzoate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pciM

query
sites
3pciM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)
binding 3-iodo-4-hydroxybenzoic acid: Y105 (= Y106), Y144 (= Y145), H159 (= H160)

3pchM Structure of protocatechuate 3,4-dioxygenase complexed with 3-chloro- 4-hydroxybenzoate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pchM

query
sites
3pchM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y
|
Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q
|
Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding 3-chloro-4-hydroxybenzoic acid: Y24 (= Y22), Y144 (= Y145), W146 (= W147), R154 (= R155), H157 (= H158), H159 (= H160), Q174 (= Q175), I188 (= I189)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)

3pcgM Structure of protocatechuate 3,4-dioxygenase complexed with the inhibitor 4-hydroxyphenylacetate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pcgM

query
sites
3pcgM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y
|
Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding 4-hydroxyphenylacetate: Y24 (= Y22), Y105 (= Y106), Y144 (= Y145), W146 (= W147), R154 (= R155), H159 (= H160), I188 (= I189)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)

3pcfM Structure of protocatechuate 3,4-dioxygenase complexed with 3-fluro-4- hydroxybenzoate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pcfM

query
sites
3pcfM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P
|
P

G

D

Y
|
Y

G

K

S

T

S

S

V
x
I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)
binding 3-fluoro-4-hydroxybenzoic acid: P22 (= P20), Y24 (= Y22), I28 (≠ V26), Y144 (= Y145), W146 (= W147), R154 (= R155), H157 (= H158), H159 (= H160), I188 (= I189)

3pceM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxyphenylacetate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pceM

query
sites
3pceM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding 3-hydroxyphenylacetate: Y105 (= Y106), Y144 (= Y145), W146 (= W147), R154 (= R155), H159 (= H160), I188 (= I189)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)

3pcbM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxybenzoate (see paper)
48% identity, 99% coverage: 2:233/234 of query aligns to 4:233/233 of 3pcbM

query
sites
3pcbM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y
|
Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

-

R

-

N

-

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I
|
I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

active site: Y105 (= Y106), Y144 (= Y145), R154 (= R155), H157 (= H158), H159 (= H160)
binding 3-hydroxybenzoic acid: Y24 (= Y22), Y144 (= Y145), W146 (= W147), R154 (= R155), I188 (= I189)
binding fe (iii) ion: Y105 (= Y106), Y144 (= Y145), H157 (= H158), H159 (= H160)

3mv4M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
47% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 3mv4M

query
sites
3mv4M

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

N

R

N

N

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W

W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

binding carbonate ion: Y108 (= Y106), H147 (≠ Y145), R157 (= R155), Y162 (≠ H160)
binding fe (iii) ion: Y108 (= Y106), H147 (≠ Y145), H160 (= H158), Y162 (≠ H160)

3mi5M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
47% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 3mi5M

query
sites
3mi5M

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

N

R

N

N

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

binding catechol: Y108 (= Y106), H147 (≠ Y145), W149 (= W147), R157 (= R155), H160 (= H158), Y162 (≠ H160)
binding fe (iii) ion: Y108 (= Y106), H160 (= H158), Y162 (≠ H160)

3mflM Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
47% identity, 99% coverage: 2:233/234 of query aligns to 4:236/238 of 3mflM

query
sites
3mflM

D

D

D

N

S

S

S

R

L

F

S

V

A

I

R

R

D

D

F

R

P

N

S

W

H

H

P

P

P

K

Y

A

V

L

Y

T

P

P

G

D

Y

Y

G

K

S

T

S

S

V

I

K

A

R

R

G

S

P

P

T

R

L

Q

P

A

L

L

I

V

P

S

L

I

K

P

E

Q

K

S

L

I

R

S

D

E

Q

T

R

T

V

G

P

P

V

N

Y

F

G

S

T

H

D

L

D

G

L

F

G

G

A

A

L

H

D

D

H

H

D

D

L

L

T

L

R

L

N

N

A

F

V

N

R

N

N

G

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

I

I

I

V

V

T

A

G

G

R

R

V

V

L

V

D

D

E

Q

G

Y

N

G

R

K

P

P

V

V

R

P

N

N

T

T

L

L

V

V

E

E

I

M

W

W

Q

Q

A

A

N

N

A

A

A

G

G

G

R

R

Y
|
Y

V

R

H

H

K

K

A

N

D

D

Q

R

H

Y

D

L

A

A

P

P

L

L

D

D

P

P

N

N

F

F

L

G

G

G

A

V

G

G

R

R

C

C

I

L

T

T

D

D

N

S

E

D

G

G

R

Y

Y

Y

R

S

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
x
H

P

P

W
|
W

G

R

N

N

H

G

P

P

N

N

A

D

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
x
Y

F

F

S

G

L

I

F

S

G

G

D

P

H

S

F

I

G

A

S

T

R

K

L

L

V

I

T

T

Q

Q

M

L

Y

Y

F

F

P

E

G

G

D

D

P

P

L

L

L

I

A

P

F

M

D

C

P

P

I

I

F

V

Q

K

-

S

-

I

G

A

T

N

P

P

E

E

H

-

A

A

R

V

D

Q

R

Q

L

L

I

I

A

A

D

K

F

L

S

D

L

M

D

N

T

N

T

A

Q

N

E

P

A

M

Y

D

A

C

L

L

G

A

Y

Y

D

R

F

F

D

D

I

I

V

V

L

L

R

R

G

G

R

Q

N

R

E

K

T

T

P

H

M

F

E

E

binding 2-(3,4-dihydroxyphenyl)acetic acid: Y108 (= Y106), H147 (≠ Y145), W149 (= W147), R157 (= R155), H160 (= H158), Y162 (≠ H160)
binding fe (iii) ion: Y108 (= Y106), H160 (= H158), Y162 (≠ H160)

2buqB Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 in complex with catechol (see paper)
48% identity, 98% coverage: 5:233/234 of query aligns to 5:234/238 of 2buqB

query
sites
2buqB

S

A

L

Y

S

A

A

Q

R

R

D

N

F

T

P

E

S

D

H

H

P

P

P

P

Y

A

V

Y

Y

A

P

P

G

G

Y

Y

G

K

S

T

S

S

V

V

K

L

R

R

G

S

P

P

T

K

L

N

P

A

L

L

I

I

P

S

L

I

K

A

E

E

K

T

L

L

R

S

D

E

Q

V

R

T

V

A

P

P

V

H

Y

F

G

S

T

A

D

D

D

K

L

F

G

G

A

P

L

K

D

D

H

N

D

D

L

L

T

I

R

L

N

N

A

Y

V

A

R

K

N

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

V

V

T

H

G

G

R

Y

V

V

L

R

D

D

E

Q

G

F

N

G

R

R

P

P

V

V

R

K

N

N

T

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

V

R

H

H

K

P

A

N

D

D

Q

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

L

G

G

G

A

C

G

G

R

R

C

M

I

L

T

T

D

D

N

D

E

N

G

G

R

Y

Y

Y

R

V

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

R

P

I

N

N

A

E

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

D

D

H

G

F

W

G

A

S

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

A

D

F

S

D

C

P

P

I

I

F

L

Q

K

G

T

T

I

P

P

-

S

E

E

H

Q

A

Q

R

R

D

R

R

A

L

L

I

I

A

A

D

L

F

E

S

D

L

K

D

S

T

N

T

F

Q

I

E

E

A

A

Y

D

A

S

L

R

G

C

Y

Y

D

R

F

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

N

R

E

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y106), Y145 (= Y145), R155 (= R155), H158 (= H158), H160 (= H160)
binding catechol: Y106 (= Y106), R155 (= R155), H158 (= H158)
binding fe (iii) ion: Y106 (= Y106), H158 (= H158), H160 (= H160)

2bumB Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 (see paper)
48% identity, 98% coverage: 5:233/234 of query aligns to 5:234/238 of 2bumB

query
sites
2bumB

S

A

L

Y

S

A

A

Q

R

R

D

N

F

T

P

E

S

D

H

H

P

P

P

P

Y

A

V

Y

Y

A

P

P

G

G

Y

Y

G

K

S

T

S

S

V

V

K

L

R

R

G

S

P

P

T

K

L

N

P

A

L

L

I

I

P

S

L

I

K

A

E

E

K

T

L

L

R

S

D

E

Q

V

R

T

V

A

P

P

V

H

Y

F

G

S

T

A

D

D

D

K

L

F

G

G

A

P

L

K

D

D

H

N

D

D

L

L

T

I

R

L

N

N

A

Y

V

A

R

K

N

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

V

V

T

H

G

G

R

Y

V

V

L

R

D

D

E

Q

G

F

N

G

R

R

P

P

V

V

R

K

N

N

T

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

V

R

H

H

K

P

A

N

D

D

Q

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

L

G

G

G

A

C

G

G

R

R

C

M

I

L

T

T

D

D

N

D

E

N

G

G

R

Y

Y

Y

R

V

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W

W

G

R

N

N

H

R

P

I

N

N

A

E

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

D

D

H

G

F

W

G

A

S

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

A

D

F

S

D

C

P

P

I

I

F

L

Q

K

G

T

T

I

P

P

-

S

E

E

H

Q

A

Q

R

R

D

R

R

A

L

L

I

I

A

A

D

L

F

E

S

D

L

K

D

S

T

N

T

F

Q

I

E

E

A

A

Y

D

A

S

L

R

G

C

Y

Y

D

R

F

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

N

R

E

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y106), Y145 (= Y145), R155 (= R155), H158 (= H158), H160 (= H160)
binding fe (iii) ion: Y106 (= Y106), Y145 (= Y145), H158 (= H158), H160 (= H160)

1eocB Crystal structure of acinetobacter sp. Adp1 protocatechuate 3,4- dioxygenase in complex with 4-nitrocatechol (see paper)
48% identity, 98% coverage: 5:233/234 of query aligns to 5:234/238 of 1eocB

query
sites
1eocB

S

A

L

Y

S

A

A

Q

R

R

D

N

F

T

P

E

S

D

H

H

P

P

P

P

Y

A

V

Y

Y

A

P

P

G

G

Y
|
Y

G

K

S

T

S

S

V

V

K

L

R

R

G

S

P

P

T

K

L

N

P

A

L

L

I

I

P

S

L

I

K

A

E

E

K

T

L

L

R

S

D

E

Q

V

R

T

V

A

P

P

V

H

Y

F

G

S

T

A

D

D

D

K

L

F

G

G

A

P

L

K

D

D

H

N

D

D

L

L

T

I

R

L

N

N

A

Y

V

A

R

K

N

D

G

G

E

L

P

P

L

I

G

G

E

E

R

R

I

V

I

I

V

V

T

H

G

G

R

Y

V

V

L

R

D

D

E

Q

G

F

N

G

R

R

P

P

V

V

R

K

N

N

T

A

L

L

V

V

E

E

I

V

W

W

Q

Q

A

A

N

N

A

A

A

S

G

G

R

R

Y
|
Y

V

R

H

H

K

P

A

N

D

D

Q

Q

H

Y

D

I

A

G

P

A

L

M

D

D

P

P

N

N

F

F

L

G

G

G

A

C

G

G

R

R

C

M

I

L

T

T

D

D

N

D

E

N

G

G

R

Y

Y

Y

R

V

F

F

L

R

T

T

I

I

K

K

P

P

G

G

A

P

Y
|
Y

P

P

W
|
W

G

R

N

N

H

R

P

I

N

N

A

E

W

W

R
|
R

P

P

N

A

H
|
H

I

I

H
|
H

F

F

S

S

L

L

F

I

G

A

D

D

H

G

F

W

G

A

S

Q

R

R

L

L

V

I

T

S

Q

Q

M

F

Y

Y

F

F

P

E

G

G

D

D

P

T

L

L

L

I

A

D

F

S

D

C

P

P

I
|
I

F

L

Q

K

G

T

T

I

P

P

-

S

E

E

H

Q

A

Q

R

R

D

R

R

A

L

L

I

I

A

A

D

L

F

E

S

D

L

K

D

S

T

N

T

F

Q

I

E

E

A

A

Y

D

A

S

L

R

G

C

Y

Y

D

R

F

F

D

D

I

I

V

T

L

L

R

R

G

G

R

R

N

R

E

A

T

T

P

Y

M

F

E

E

active site: Y106 (= Y106), Y145 (= Y145), R155 (= R155), H158 (= H158), H160 (= H160)
binding 4-nitrocatechol: Y22 (= Y22), Y106 (= Y106), W147 (= W147), R155 (= R155), H158 (= H158), H160 (= H160), I189 (= I189)
binding fe (iii) ion: Y106 (= Y106), H158 (= H158), H160 (= H160)

Query Sequence

>BPHYT_RS33455 FitnessBrowser__BFirm:BPHYT_RS33455
MDDSSLSARDFPSHPPYVYPGYGSSVKRGPTLPLIPLKEKLRDQRVPVYGTDDLGALDHD
LTRNAVRNGEPLGERIIVTGRVLDEGNRPVRNTLVEIWQANAAGRYVHKADQHDAPLDPN
FLGAGRCITDNEGRYRFLTIKPGAYPWGNHPNAWRPNHIHFSLFGDHFGSRLVTQMYFPG
DPLLAFDPIFQGTPEHARDRLIADFSLDTTQEAYALGYDFDIVLRGRNETPMER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory