SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BT4313 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8rd8VH Small ribosomal subunit protein uS5 (see paper)
68% identity, 90% coverage: 2:81/89 of query aligns to 1:81/84 of 8rd8VH

query
sites
8rd8VH

A

A

H

H

K

K

K

K

G

A

V

A

G

G

S

S

S
|
S

K
x
R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
N

S
x
P

K
|
K

R
x
M

L

L

G

G

V

V

K
|
K

I

I

F
|
F

G
|
G

G

G

E
x
Q

A

A

C

I

K

V

A
|
A

G
|
G

N
|
N

I
|
I

I

I

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
|
E

F

F

H
|
H

P

A

G

G

E

A

N

G

I

V

G

G

M

M

G
|
G

K
x
R

D
|
D

H
|
H

T

T

L

L

F
|
F

A

A

L

L

V

N

D

D

G

G

T

V

V

V

K

K

F
|
F

K

A

V

T

-

K

G
|
G

R
x
K

E
x
F

D
x
N

R
|
R

R
|
R

Y

Y

V

V

S

M

I

V

binding : S9 (= S10), R10 (≠ K11), N11 (= N12), R13 (= R14), D14 (≠ E15), S15 (= S16), N16 (≠ H17), P17 (≠ S18), K18 (= K19), M19 (≠ R20), K23 (= K24), F25 (= F26), G26 (= G27), Q28 (≠ E29), A32 (= A33), G33 (= G34), N34 (= N35), I35 (= I36), R38 (= R39), R40 (= R41), G41 (= G42), T42 (= T43), E43 (= E44), H45 (= H46), G53 (= G54), R54 (≠ K55), D55 (= D56), H56 (= H57), F59 (= F60), F68 (= F69), G72 (= G72), K73 (≠ R73), F74 (≠ E74), N75 (≠ D75), R76 (= R76), R77 (= R77)

6eriAW 50S ribosomal protein L4, chloroplastic (see paper)
62% identity, 92% coverage: 4:85/89 of query aligns to 1:82/114 of 6eriAW

query
sites
6eriAW

K
|
K

K

K

G

G

V

A

G

G

S
|
S

S
x
T

K

K

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H

K

S
x
G

K
x
Q

R
|
R

L

L

G

G

V

V

K
|
K

I

I

F
x
Y

G
|
G

G

D

E
x
Q

A

V

C

A

K
|
K

A

P

G

G

N

A

I
|
I

I

I

I

I

R
|
R

Q

Q

R
|
R

G

G

T
|
T

E

K

F

F

H
|
H

P

P

G

G

E

K

N

N

I

V

G

G

M

I

G
|
G

K
|
K

D
|
D

H

H

T
|
T

L

I

F

F

A

A

L

L

V

I

D

D

G

G

T

L

V

V

K

K

F
|
F

K

E

V
x
K

G

Y

R
x
G

E

P

D

D

R
x
K

R

K

Y

K

V

V

S

S

I

V

I

Y

P

P

A

R

E

E

binding : K1 (= K4), S6 (= S9), T7 (≠ S10), N9 (= N12), R11 (= R14), D12 (≠ E15), S13 (= S16), G15 (≠ S18), Q16 (≠ K19), R17 (= R20), K21 (= K24), Y23 (≠ F26), G24 (= G27), Q26 (≠ E29), K29 (= K32), I33 (= I36), R36 (= R39), R38 (= R41), T40 (= T43), H43 (= H46), G51 (= G54), K52 (= K55), D53 (= D56), T55 (= T58), F66 (= F69), K68 (≠ V71), G70 (≠ R73), K73 (≠ R76)

Sites not aligning to the query:

binding : 91, 94, 95, 99, 103, 106, 109, 113

4v61BX Ribosomal Protein S8 (see paper)
60% identity, 94% coverage: 2:85/89 of query aligns to 1:84/86 of 4v61BX

query
sites
4v61BX

A
|
A

H
|
H

K
|
K

K

K

G

G

V

A

G

G

S
|
S

S
x
T

K
|
K

N

N

G

G

R
|
R

E
x
D

S

S

H
x
P

S
x
G

K
x
Q

R
|
R

L
|
L

G

G

V
|
V

K
|
K

I
|
I

F
x
Y

G

G

G

D

E

Q

A
x
V

C
x
A

K
|
K

A

P

G

G

N

A

I

I

I

I

I
x
V

R
|
R

Q
|
Q

R
|
R

G

G

T

T

E

K

F

F

H

H

P

A

G

G

E

K

N
|
N

I

V

G

G

M

I

G

G

K

K

D

D

H

H

T

T

L

I

F

F

A

S

L

L

V

I

D
|
D

G

G

T
x
L

V

V

K

K

F

F

K
x
E

V

K

G
x
F

R

G

E

P

D

D

R

R

R

K

Y

K

V

I

S

S

I

V

I

Y

P

P

A

R

E

E

binding : A1 (= A2), H2 (= H3), K3 (= K4), S8 (= S9), T9 (≠ S10), K10 (= K11), R13 (= R14), D14 (≠ E15), P16 (≠ H17), G17 (≠ S18), Q18 (≠ K19), R19 (= R20), L20 (= L21), V22 (= V23), K23 (= K24), I24 (= I25), Y25 (≠ F26), V29 (≠ A30), A30 (≠ C31), K31 (= K32), V37 (≠ I38), R38 (= R39), Q39 (= Q40), R40 (= R41), N49 (= N50), D63 (= D64), L65 (≠ T66), E69 (≠ K70), F71 (≠ G72)

5aa0AX of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP (see paper)
60% identity, 93% coverage: 1:83/89 of query aligns to 1:83/85 of 5aa0AX

query
sites
5aa0AX

M
|
M

A
|
A

H
|
H

K
|
K

K

K

G
|
G

V
x
L

G

G

S
|
S

S

T

K
x
R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
Q

S
x
A

K
|
K

R
|
R

L

L

G

G

V
|
V

K
|
K

I
x
R

F
x
Y

G
x
E

G

G

E
x
Q

A

V

C

V

K
x
R

A
|
A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
x
R

F

F

H

K

P

P

G

G

E

K

N

N

I

V

G

G

M

M

G
|
G

K
x
R

D
|
D

H
x
F

T
|
T

L

L

F
|
F

A

A

L

L

V

V

D

D

G

G

T

V

V

V

K

E

F
|
F

K

-

V

-

G

-

R

-

E

Q

D

D

R

R

-

G

-
x
R

-
x
L

-

G

R
|
R

Y

Y

V

V

S

H

I

V

I

R

P

P

binding 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate): A2 (= A2), K4 (= K4)
binding : M1 (= M1), A2 (= A2), H3 (= H3), K4 (= K4), G6 (= G6), L7 (≠ V7), L7 (≠ V7), S9 (= S9), R11 (≠ K11), N12 (= N12), R14 (= R14), D15 (≠ E15), S16 (= S16), Q17 (≠ H17), A18 (≠ S18), K19 (= K19), R20 (= R20), V23 (= V23), K24 (= K24), R25 (≠ I25), Y26 (≠ F26), E27 (≠ G27), Q29 (≠ E29), R32 (≠ K32), A33 (= A33), G34 (= G34), N35 (= N35), I36 (= I36), R39 (= R39), R41 (= R41), G42 (= G42), T43 (= T43), R44 (≠ E44), G54 (= G54), R55 (≠ K55), D56 (= D56), F57 (≠ H57), T58 (= T58), F60 (= F60), F69 (= F69), R74 (vs. gap), L75 (vs. gap), R77 (= R77)

5a9zAX of Thermous thermophilus ribosome bound to BipA-GDPCP (see paper)
60% identity, 93% coverage: 1:83/89 of query aligns to 1:83/85 of 5a9zAX

query
sites
5a9zAX

M
|
M

A
|
A

H
|
H

K

K

K

K

G

G

V
x
L

G

G

S
|
S

S

T

K
x
R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
Q

S
x
A

K
|
K

R
|
R

L

L

G

G

V
|
V

K
|
K

I
x
R

F
x
Y

G
x
E

G

G

E
x
Q

A

V

C

V

K
x
R

A
|
A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
x
R

F

F

H

K

P

P

G

G

E

K

N

N

I

V

G

G

M

M

G
|
G

K
x
R

D
|
D

H
x
F

T
|
T

L

L

F
|
F

A

A

L

L

V

V

D

D

G

G

T

V

V

V

K

E

F
|
F

K

-

V

-

G

-

R

-

E

Q

D

D

R

R

-

G

-
x
R

-
x
L

-

G

R
|
R

Y

Y

V

V

S

H

I

V

I

R

P

P

binding : M1 (= M1), A2 (= A2), H3 (= H3), L7 (≠ V7), S9 (= S9), R11 (≠ K11), N12 (= N12), R14 (= R14), D15 (≠ E15), S16 (= S16), Q17 (≠ H17), A18 (≠ S18), K19 (= K19), R20 (= R20), V23 (= V23), K24 (= K24), R25 (≠ I25), Y26 (≠ F26), E27 (≠ G27), Q29 (≠ E29), R32 (≠ K32), A33 (= A33), G34 (= G34), N35 (= N35), I36 (= I36), R39 (= R39), R41 (= R41), G42 (= G42), T43 (= T43), R44 (≠ E44), G54 (= G54), R55 (≠ K55), D56 (= D56), F57 (≠ H57), T58 (= T58), F60 (= F60), F69 (= F69), R74 (vs. gap), L75 (vs. gap), R77 (= R77)

7uvvV 50S ribosomal protein L27 (see paper)
64% identity, 90% coverage: 2:81/89 of query aligns to 1:81/83 of 7uvvV

query
sites
7uvvV

A
|
A

H
x
T

K

K

K
|
K

G

A

V
x
G

G

G

S

S

S

T

K

K

N
|
N

G

G

R
|
R

E

D

S
|
S

H
x
N

S
x
P

K
|
K

R
x
M

L

L

G

G

V

V

K

K

I

V

F
x
Y

G
|
G

G

G

E
x
Q

A

T

C

V

K

T

A
|
A

G
|
G

N
|
N

I
|
I

I

I

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E

E

F

F

H

H

P

A

G

G

E

A

N

N

I

V

G

G

M

M

G
|
G

K
x
R

D

D

H
|
H

T

T

L

L

F
|
F

A

A

L

T

V

A

D

D

G

G

T

V

V

V

K

K

F

F

K

E

V

V

-

K

G

G

R

Q

E

F

D

G

R

R

R
|
R

Y

Y

V

V

S

K

I

V

binding : A1 (= A2), T2 (≠ H3), T2 (≠ H3), K4 (= K5), G6 (≠ V7), N11 (= N12), R13 (= R14), S15 (= S16), N16 (≠ H17), P17 (≠ S18), K18 (= K19), M19 (≠ R20), Y25 (≠ F26), G26 (= G27), Q28 (≠ E29), A32 (= A33), G33 (= G34), N34 (= N35), I35 (= I36), R38 (= R39), R40 (= R41), G41 (= G42), T42 (= T43), G53 (= G54), R54 (≠ K55), H56 (= H57), F59 (= F60), R77 (= R77)

4v4gBX 50S ribosomal protein L27 (see paper)
63% identity, 94% coverage: 2:85/89 of query aligns to 1:84/86 of 4v4gBX

query
sites
4v4gBX

A

A

H

H

K
|
K

K

K

G

G

V

V

G

G

S
|
S

S
|
S

K
|
K

N
|
N

G

G

R
|
R

E

D

S

S

H

N

S
x
P

K
|
K

R
x
Y

L
|
L

G

G

V
|
V

K
|
K

I
x
K

F
|
F

G

G

G

G

E
|
E

A
x
V

C
x
V

K
|
K

A

A

G

G

N

N

I

I

I
x
L

I
x
V

R
|
R

Q
|
Q

R
|
R

G
|
G

T

T

E

K

F

F

H

K

P

A

G

G

E

Q

N

G

I
x
V

G

G

M

M

G

G

K

R

D
|
D

H
|
H

T

T

L

L

F

F

A

A

L

L

V

S

D

D

G

G

T
x
K

V

V

K

V

F
|
F

K
x
I

V
x
N

G

K

R

G

E

K

D

G

R

A

R

R

Y

F

V

I

S

S

I

I

I

E

P

A

A

A

E

Q

binding : K3 (= K4), S8 (= S9), S9 (= S10), K10 (= K11), N11 (= N12), R13 (= R14), P17 (≠ S18), K18 (= K19), Y19 (≠ R20), L20 (= L21), V22 (= V23), K23 (= K24), K24 (≠ I25), F25 (= F26), E28 (= E29), V29 (≠ A30), V30 (≠ C31), K31 (= K32), L36 (≠ I37), V37 (≠ I38), R38 (= R39), Q39 (= Q40), R40 (= R41), G41 (= G42), V50 (≠ I51), D55 (= D56), H56 (= H57), K65 (≠ T66), F68 (= F69), I69 (≠ K70), N70 (≠ V71)

1vy4B0 50S ribosomal protein L36 (see paper)
59% identity, 92% coverage: 2:83/89 of query aligns to 1:82/83 of 1vy4B0

query
sites
1vy4B0

A
|
A

H
|
H

K
|
K

K

K

G

G

V

L

G

G

S
|
S

S
x
T

K

R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
Q

S
x
A

K
|
K

R
|
R

L

L

G
|
G

V
|
V

K
|
K

I
x
R

F
x
Y

G
x
E

G

G

E
x
Q

A

V

C

V

K
x
R

A
|
A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E

R

F

F

H

K

P

P

G

G

E

K

N

N

I

V

G

G

M

M

G
|
G

K
x
R

D
|
D

H
x
F

T

T

L

L

F
|
F

A

A

L

L

V

V

D

D

G

G

T

V

V

V

K

E

F
|
F

K

-

V

-

G

-

R

-

E

Q

D

D

R
|
R

-
x
G

-
x
R

-
x
L

-

G

R
|
R

Y

Y

V

V

S

H

I

V

I

R

P

P

binding magnesium ion: G21 (= G22), V22 (= V23)
binding : A1 (= A2), H2 (= H3), K3 (= K4), S8 (= S9), T9 (≠ S10), N11 (= N12), R13 (= R14), D14 (≠ E15), S15 (= S16), Q16 (≠ H17), A17 (≠ S18), K18 (= K19), R19 (= R20), K23 (= K24), R24 (≠ I25), Y25 (≠ F26), E26 (≠ G27), Q28 (≠ E29), R31 (≠ K32), A32 (= A33), G33 (= G34), N34 (= N35), I35 (= I36), R38 (= R39), R40 (= R41), G41 (= G42), T42 (= T43), G53 (= G54), R54 (≠ K55), D55 (= D56), F56 (≠ H57), F59 (= F60), F68 (= F69), R71 (= R76), G72 (vs. gap), R73 (vs. gap), R73 (vs. gap), L74 (vs. gap), R76 (= R77)

8cd1W phikz014 (see paper)
66% identity, 84% coverage: 10:84/89 of query aligns to 1:76/76 of 8cd1W

query
sites
8cd1W

S

T

K

R

N
|
N

G

G

R
|
R

E

D

S
|
S

H
x
E

S
|
S

K
|
K

R
|
R

L
|
L

G

G

V

V

K

K

I

L

F
|
F

G
|
G

G
|
G

E
x
Q

A

A

C

V

K

K

A

A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
x
K

F

F

H
|
H

P

A

G

G

E

Y

N

G

I

V

G

G

M

L

G
|
G

K
|
K

D
|
D

H
|
H

T

T

L

L

F
|
F

A

A

L

K

V

V

D

D

G

G

T

V

V

V

K

K

F
|
F

K

E

V

T

-
x
K

G
|
G

R

A

E
x
F

D

G

R

R

R
x
K

Y

Y

V

V

S

S

I

I

I

V

P

A

A

A

binding : N3 (= N12), R5 (= R14), S7 (= S16), E8 (≠ H17), S9 (= S18), K10 (= K19), R11 (= R20), L12 (= L21), F17 (= F26), G18 (= G27), G19 (= G28), Q20 (≠ E29), G25 (= G34), N26 (= N35), I27 (= I36), R30 (= R39), R32 (= R41), G33 (= G42), T34 (= T43), K35 (≠ E44), H37 (= H46), G45 (= G54), K46 (= K55), D47 (= D56), H48 (= H57), F51 (= F60), F60 (= F69), K63 (vs. gap), G64 (= G72), F66 (≠ E74), K69 (≠ R77)

7unrY 30S ribosomal protein S6
66% identity, 84% coverage: 10:84/89 of query aligns to 1:76/76 of 7unrY

query
sites
7unrY

S

T

K
x
R

N
|
N

G
|
G

R
|
R

E

D

S
|
S

H

E

S
|
S

K
|
K

R
|
R

L

L

G

G

V

V

K
|
K

I

L

F
|
F

G
|
G

G

G

E
x
Q

A

A

C

V

K
|
K

A
|
A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
x
K

F

F

H

H

P

A

G

G

E

Y

N

G

I

V

G

G

M

L

G
|
G

K
|
K

D
|
D

H
|
H

T

T

L

L

F
|
F

A

A

L

K

V

V

D

D

G

G

T

V

V

V

K

K

F

F

K

E

V

T

-
x
K

G
|
G

R

A

E

F

D

G

R
|
R

R
x
K

Y

Y

V

V

S

S

I

I

I

V

P

A

A

A

binding magnesium ion: N3 (= N12), G4 (= G13)
binding : R2 (≠ K11), N3 (= N12), R5 (= R14), S7 (= S16), S9 (= S18), K10 (= K19), R11 (= R20), K15 (= K24), F17 (= F26), G18 (= G27), Q20 (≠ E29), K23 (= K32), A24 (= A33), G25 (= G34), N26 (= N35), I27 (= I36), R30 (= R39), R32 (= R41), G33 (= G42), T34 (= T43), K35 (≠ E44), G45 (= G54), K46 (= K55), D47 (= D56), H48 (= H57), F51 (= F60), K63 (vs. gap), G64 (= G72), R68 (= R76), K69 (≠ R77)

8a3lv 50S ribosomal protein L36 (see paper)
63% identity, 90% coverage: 2:81/89 of query aligns to 1:81/84 of 8a3lv

query
sites
8a3lv

A
|
A

H
|
H

K
|
K

K
|
K

G

A

V

G

G

G

S
|
S

S
x
T

K

R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
E

S
x
A

K
|
K

R
|
R

L
|
L

G

G

V

V

K
|
K

I
x
R

F
|
F

G
|
G

G

G

E
|
E

A

S

C

V

K
x
L

A
|
A

G
|
G

N
x
S

I
|
I

I

I

I

V

R

R

Q

Q

R
|
R

G
|
G

T
|
T

E

K

F
|
F

H
|
H

P

A

G

G

E

A

N

N

I

V

G

G

M

C

G
|
G

K
x
R

D
|
D

H
|
H

T
|
T

L

L

F
|
F

A

A

L

K

V

A

D

D

G

G

T

K

V

V

K

K

F
|
F

K

E

V

V

-

K

G
|
G

R
x
P

E
x
K

D

N

R
|
R

R
x
K

Y

F

V

I

S

S

I

I

binding : A1 (= A2), H2 (= H3), K3 (= K4), K4 (= K5), S8 (= S9), T9 (≠ S10), N11 (= N12), R13 (= R14), D14 (≠ E15), S15 (= S16), E16 (≠ H17), A17 (≠ S18), K18 (= K19), R19 (= R20), L20 (= L21), K23 (= K24), R24 (≠ I25), F25 (= F26), G26 (= G27), E28 (= E29), L31 (≠ K32), A32 (= A33), G33 (= G34), S34 (≠ N35), I35 (= I36), R40 (= R41), G41 (= G42), T42 (= T43), F44 (= F45), H45 (= H46), G53 (= G54), R54 (≠ K55), D55 (= D56), H56 (= H57), T57 (= T58), F59 (= F60), F68 (= F69), G72 (= G72), P73 (≠ R73), K74 (≠ E74), R76 (= R76), K77 (≠ R77)

7jilW 50S ribosomal protein L15 (see paper)
66% identity, 83% coverage: 10:83/89 of query aligns to 1:74/75 of 7jilW

query
sites
7jilW

S

S

K

K

N
|
N

G

G

R
|
R

E
|
E

S
|
S

H
x
E

S
|
S

K
|
K

R
|
R

L
|
L

G

G

V

V

K

K

I

I

F
x
Y

G

G

G

G

E
x
Q

A

A

C

A

K
x
I

A
|
A

G
|
G

N
|
N

I
|
I

I

I

I

V

R

R

Q

Q

R
|
R

G

G

T
x
S

E

K

F

H

H

N

P

P

G

G

E

E

N

N

I

V

G
x
Y

M

I

G
x
S

K
|
K

D

D

H
|
H

T

T

L

L

F
x
H

A

A

L

R

V

V

D

A

G

G

T

V

V

V

K

K

F
|
F

K

Q

V

K

G

K

R
|
R

E

D

D

N

R
x
K

R

S

Y

Y

V

V

S

S

I

I

I

I

P

P

binding : N3 (= N12), R5 (= R14), E6 (= E15), S7 (= S16), E8 (≠ H17), S9 (= S18), K10 (= K19), R11 (= R20), L12 (= L21), Y17 (≠ F26), Q20 (≠ E29), I23 (≠ K32), A24 (= A33), G25 (= G34), N26 (= N35), I27 (= I36), R32 (= R41), S34 (≠ T43), Y43 (≠ G52), S45 (≠ G54), K46 (= K55), H48 (= H57), H51 (≠ F60), F60 (= F69), R64 (= R73), K67 (≠ R76)

8v9kY 50S ribosomal protein L27 (see paper)
60% identity, 92% coverage: 2:83/89 of query aligns to 1:81/85 of 8v9kY

query
sites
8v9kY

A

A

H

H

K

K

K

K

G

G

V

A

G

S

S
|
S

S

S

K

R

N
|
N

G

G

R
|
R

E

D

S
|
S

H

A

S
x
A

K

Q

R
|
R

L

L

G

G

V

V

K

K

I

R

F
|
F

G
|
G

G

G

E
x
Q

A

V

C

V

K
|
K

A
|
A

G
|
G

N

E

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E

H

F

F

H

H

P

P

G

G

E

V

N

N

I

V

G

G

M

R

G
|
G

K
x
G

D
|
D

H

D

T

T

L

L

F
|
F

A

A

L

L

V

A

D
x
P

G

G

T

A

V

V

K

E

F
|
F

K

G

V

A

G

K

R

R

E

-

D

G

R
|
R

R
x
K

Y

T

V

V

S

N

I

I

I

V

P

P

binding : S8 (= S9), N11 (= N12), R13 (= R14), S15 (= S16), A17 (≠ S18), R19 (= R20), F25 (= F26), G26 (= G27), Q28 (≠ E29), K31 (= K32), A32 (= A33), G33 (= G34), I35 (= I36), R38 (= R39), R40 (= R41), G41 (= G42), T42 (= T43), G53 (= G54), G54 (≠ K55), D55 (= D56), F59 (= F60), P63 (≠ D64), F68 (= F69), R74 (= R76), K75 (≠ R77)

Sites not aligning to the query:

binding : 84

4v85B0 50S ribosomal protein L17 (see paper)
63% identity, 83% coverage: 8:81/89 of query aligns to 2:76/79 of 4v85B0

query
sites
4v85B0

G

G

S
|
S

S

T

K
x
R

N
|
N

G
|
G

R

R

E
x
D

S

S

H
x
E

S

A

K
|
K

R
|
R

L
|
L

G

G

V
|
V

K
|
K

I
x
R

F
|
F

G

G

G

G

E
|
E

A

S

C
x
V

K
x
L

A
|
A

G

G

N

S

I

I

I

I

I
x
V

R
|
R

Q
|
Q

R
|
R

G
|
G

T

T

E

K

F
|
F

H

H

P

A

G

G

E

A

N

N

I

V

G

G

M

C

G
|
G

K
x
R

D
|
D

H
|
H

T

T

L

L

F
|
F

A

A

L

K

V

A

D

D

G

G

T

K

V

V

K

K

F
|
F

K

E

V

V

-

K

G

G

R

P

E

K

D

N

R
|
R

R

K

Y

F

V

I

S

S

I

I

binding magnesium ion: V32 (≠ I38), R33 (= R39)
binding : S3 (= S9), R5 (≠ K11), N6 (= N12), G7 (= G13), D9 (≠ E15), E11 (≠ H17), K13 (= K19), R14 (= R20), L15 (= L21), V17 (= V23), K18 (= K24), R19 (≠ I25), F20 (= F26), E23 (= E29), V25 (≠ C31), L26 (≠ K32), A27 (= A33), R33 (= R39), Q34 (= Q40), R35 (= R41), G36 (= G42), F39 (= F45), G48 (= G54), R49 (≠ K55), D50 (= D56), H51 (= H57), F54 (= F60), F63 (= F69), R71 (= R76)

8qcqW Large ribosomal subunit protein bL31-A (see paper)
57% identity, 92% coverage: 2:83/89 of query aligns to 1:82/85 of 8qcqW

query
sites
8qcqW

A
|
A

H
x
S

K
|
K

K

K

G
|
G

V
|
V

G

G

S
|
S

S
x
T

K

K

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
E

S
x
A

K
|
K

R
|
R

L
|
L

G

G

V

A

K
|
K

I
x
R

F
x
A

G
x
D

G
|
G

E
x
Q

A

F

C

V

K

T

A
x
G

G
|
G

N
x
S

I
|
I

I

L

I
x
Y

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E
x
K

F

I

H
x
Y

P

P

G

G

E

E

N

N

I

V

G

G

M

R

G
|
G

K
x
G

D
|
D

H
x
D

T
|
T

L

L

F
|
F

A

A

L

K

V

I

D

D

G

G

T

T

V

V

K

K

F
|
F

K

E

V
x
R

G

F

R

G

E
x
R

D

D

R
|
R

R
x
K

Y

K

V

V

S

S

I

V

I

Y

P

P

binding : A1 (= A2), A1 (= A2), S2 (≠ H3), K3 (= K4), G5 (= G6), V6 (= V7), S8 (= S9), T9 (≠ S10), N11 (= N12), R13 (= R14), D14 (≠ E15), S15 (= S16), E16 (≠ H17), A17 (≠ S18), K18 (= K19), R19 (= R20), L20 (= L21), K23 (= K24), R24 (≠ I25), A25 (≠ F26), D26 (≠ G27), G27 (= G28), Q28 (≠ E29), G32 (≠ A33), G33 (= G34), S34 (≠ N35), I35 (= I36), Y37 (≠ I38), R38 (= R39), R40 (= R41), G41 (= G42), T42 (= T43), K43 (≠ E44), Y45 (≠ H46), G53 (= G54), G54 (≠ K55), D55 (= D56), D56 (≠ H57), T57 (= T58), F59 (= F60), F68 (= F69), R70 (≠ V71), R73 (≠ E74), R75 (= R76), K76 (≠ R77)

8fmwAY 30S ribosomal protein S10 (see paper)
63% identity, 84% coverage: 8:82/89 of query aligns to 1:74/74 of 8fmwAY

query
sites
8fmwAY

G

G

S
|
S

S
|
S

K

K

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
I

S
|
S

K
|
K

R
|
R

L

L

G

G

V

V

K
|
K

I
x
R

F
x
S

G
|
G

G

G

E
x
Q

A

F

C

V

K
|
K

A

A

G
|
G

N

E

I
|
I

I

I

I

V

R
|
R

Q

Q

R
|
R

G

G

T
|
T

E

K

F
|
F

H
|
H

P

K

G

G

E

K

N

N

I

V

G

G

M

L

G
|
G

K
x
R

D
|
D

H
x
Y

T

T

L

I

F
|
F

A

A

L

L

V

S

D

S

G

G

T

K

V

V

K

E

F
|
F

K

K

V

T

G

-

R
x
L

E
x
K

D

G

R
|
R

R
x
K

Y

Y

V

V

S

S

I

I

I

V

binding : S2 (= S9), S3 (= S10), N5 (= N12), R7 (= R14), D8 (≠ E15), S9 (= S16), I10 (≠ H17), S11 (= S18), K12 (= K19), R13 (= R20), K17 (= K24), R18 (≠ I25), S19 (≠ F26), G20 (= G27), Q22 (≠ E29), K25 (= K32), G27 (= G34), I29 (= I36), R32 (= R39), R34 (= R41), T36 (= T43), F38 (= F45), H39 (= H46), G47 (= G54), R48 (≠ K55), D49 (= D56), Y50 (≠ H57), F53 (= F60), F62 (= F69), L65 (≠ R73), K66 (≠ E74), R68 (= R76), K69 (≠ R77)

4v8eA3 structure analysis of ribosomal decoding (near-cognate tRNA-tyr complex). (see paper)
56% identity, 83% coverage: 10:83/89 of query aligns to 1:74/76 of 4v8eA3

query
sites
4v8eA3

S

T

K

R

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
x
Q

S
x
A

K
|
K

R
|
R

L

L

G

G

V
|
V

K
|
K

I
x
R

F
x
Y

G
x
E

G

G

E
x
Q

A

V

C

V

K
x
R

A
|
A

G
|
G

N
|
N

I
|
I

I

L

I

V

R
|
R

Q

Q

R
|
R

G
|
G

T
|
T

E

R

F

F

H

K

P

P

G

G

E

K

N

N

I

V

G

G

M

M

G
|
G

K
x
R

D
|
D

H
x
F

T

T

L

L

F
|
F

A

A

L

L

V

V

D

D

G

G

T

V

V

V

K

E

F
|
F

K

-

V

-

G

-

R

-

E

Q

D

D

R
|
R

-

G

-
x
R

-
x
L

-

G

R
|
R

Y

Y

V

V

S

H

I

V

I

R

P

P

binding osmium (III) hexammine: E18 (≠ G27), F60 (= F69)
binding : N3 (= N12), R5 (= R14), D6 (≠ E15), S7 (= S16), Q8 (≠ H17), A9 (≠ S18), K10 (= K19), R11 (= R20), V14 (= V23), K15 (= K24), R16 (≠ I25), Y17 (≠ F26), E18 (≠ G27), Q20 (≠ E29), R23 (≠ K32), A24 (= A33), G25 (= G34), N26 (= N35), I27 (= I36), R30 (= R39), R32 (= R41), G33 (= G42), T34 (= T43), G45 (= G54), R46 (≠ K55), D47 (= D56), F48 (≠ H57), F51 (= F60), F60 (= F69), R63 (= R76), R65 (vs. gap), L66 (vs. gap), R68 (= R77)

8p8wu 50S ribosomal protein L32 (see paper)
60% identity, 79% coverage: 1:70/89 of query aligns to 12:81/101 of 8p8wu

query
sites
8p8wu

M
x
F

A

A

H
x
S

K

K

K
|
K

G
|
G

V

V

G

G

S
|
S

S

T

K
|
K

N
|
N

G

G

R
|
R

E
x
D

S
|
S

H
|
H

S
x
A

K
|
K

R
|
R

L
|
L

G

G

V

A

K
|
K

I

K

F
x
A

G
x
D

G

G

E
x
Q

A

M

C

I

K

R

A

T

G
|
G

N
x
Q

I
|
I

I

I

I

Y

R
|
R

Q

Q

R
|
R

G

G

T
|
T

E

R

F

V

H

Y

P

P

G

G

E

V

N

N

I

V

G

G

M

L

G
|
G

K
x
S

D
|
D

H

D

T

T

L

L

F
|
F

A

A

L

L

V

S

D

D

G

G

T

L

V

V

K

K

F
x
Y

K

Q

binding : F12 (≠ M1), S14 (≠ H3), K16 (= K5), G17 (= G6), S20 (= S9), K22 (= K11), N23 (= N12), R25 (= R14), D26 (≠ E15), S27 (= S16), H28 (= H17), A29 (≠ S18), K30 (= K19), R31 (= R20), L32 (= L21), K35 (= K24), A37 (≠ F26), D38 (≠ G27), Q40 (≠ E29), G45 (= G34), Q46 (≠ N35), I47 (= I36), R50 (= R39), R52 (= R41), T54 (= T43), G65 (= G54), S66 (≠ K55), D67 (= D56), F71 (= F60), Y80 (≠ F69)

Sites not aligning to the query:

binding : 1, 2, 3, 5, 6, 11, 82, 85, 86, 86

Q2FXT0 Large ribosomal subunit protein bL27; 50S ribosomal protein L27 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
54% identity, 91% coverage: 1:81/89 of query aligns to 9:89/94 of Q2FXT0

query
sites
Q2FXT0

M
x
F

A

A

H

S

K

K

K

K

G

G

V

V

G

S

S

S

S

T

K

K

N

N

G

G

R

R

E

D

S

S

H

E

S

S

K

K

R

R

L

L

G

G

V

A

K

K

I

R

F

A

G

D

G

G

E

Q

A

F

C

V

K

T

A

G

G

G

N

S

I

I

I

L

I

Y

R

R

Q

Q

R

R

G

G

T

T

E

K

F

I

H

Y

P

P

G

G

E

E

N

N

I

V

G

G

M

R

G

G

K

G

D

D

H

D

T

T

L

L

F

F

A

A

L

K

V

I

D

D

G

G

T

V

V

V

K

K

F

F

K

E

V

R

G

K

R

G

E

R

D

D

R

K

R

K

Y

Q

V

V

S

S

I

V

Sites not aligning to the query:

1:9 modified: propeptide
2:9 mutation Missing: No growth, cannot replace wild-type protein.
8:9 FF→AA: No growth, cannot replace wild-type protein.

6xywAw At4g35490 (see paper)
56% identity, 83% coverage: 10:83/89 of query aligns to 1:75/76 of 6xywAw

query
sites
6xywAw

S
x
T

K

K

N
|
N

G

G

R
|
R

E

D

S
|
S

H
x
N

S
x
P

K
|
K

R
x
F

L

L

G

G

V

V

K
|
K

I

K

F
|
F

G
|
G

G

G

E
|
E

A

S

C

V

K
x
I

A
x
P

G
|
G

N

N

I
|
I

I

I

I

V

R
|
R

Q

Q

R
|
R

G

G

T
|
T

E

R

F
|
F

H

H

P

P

G

G

E

D

N

Y

I

V

G

G

M

I

G
|
G

K
|
K

D
|
D

H
|
H

T
|
T

L

L

F
|
F

A

A

L
|
L

V

K

D

E

G

G

T

R

V

V

K

R

F
|
F

K

E

V

K

G

S

R

K

-

I

E

T

D

G

R
|
R

R

K

Y

W

V

I

S

H

I

V

I

D

P

P

binding : T1 (≠ S10), N3 (= N12), R5 (= R14), S7 (= S16), N8 (≠ H17), P9 (≠ S18), K10 (= K19), F11 (≠ R20), K15 (= K24), F17 (= F26), G18 (= G27), E20 (= E29), I23 (≠ K32), P24 (≠ A33), G25 (= G34), I27 (= I36), R30 (= R39), R32 (= R41), T34 (= T43), F36 (= F45), G45 (= G54), K46 (= K55), D47 (= D56), H48 (= H57), T49 (= T58), F51 (= F60), L53 (= L62), F60 (= F69), R68 (= R76)

Query Sequence

>BT4313
MAHKKGVGSSKNGRESHSKRLGVKIFGGEACKAGNIIIRQRGTEFHPGENIGMGKDHTLF
ALVDGTVKFKVGREDRRYVSIIPAEATEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory