SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS00680 FitnessBrowser__Koxy:BWI76_RS00680 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P32171 L-Rhamnulokinase; RhaB; RhuK; ATP:L-rhamnulose phosphotransferase; L-rhamnulose 1-kinase; Rhamnulose kinase; EC 2.7.1.5 from Escherichia coli (strain K12) (see paper)
78% identity, 100% coverage: 1:488/488 of query aligns to 1:489/489 of P32171

query
sites
P32171

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ASSGR 13:17 (= ASSGR 13:17) binding
C68 (= C68) modified: Disulfide link with 222
E69 (= E69) mutation to A: Same kinase activity as the wild-type; when associated with A-70 and A-73.
E70 (≠ Q70) mutation to A: Same kinase activity as the wild-type; when associated with A-69 and A-73.
R73 (≠ L73) mutation to A: Same kinase activity as the wild-type; when associated with A-69 and A-70.
G83 (= G83) binding
C222 (= C222) modified: Disulfide link with 68
HDT 236:238 (= HDT 236:238) binding
D237 (= D237) active site, Proton acceptor
T259 (= T259) binding
N296 (= N296) binding
Q304 (= Q304) binding
C353 (= C353) modified: Disulfide link with 370
C370 (= C370) modified: Disulfide link with 353
G402 (= G402) binding

Q1R415 Rhamnulokinase; Rhamnulose kinase; EC 2.7.1.5 from Escherichia coli (strain UTI89 / UPEC) (see paper)
78% identity, 100% coverage: 1:488/488 of query aligns to 1:489/489 of Q1R415

query
sites
Q1R415

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ASSGR 13:17 (= ASSGR 13:17) binding
G83 (= G83) binding
HDT 236:238 (= HDT 236:238) binding
D237 (= D237) active site, Proton acceptor
T259 (= T259) binding
N296 (= N296) binding
Q304 (= Q304) binding
G402 (= G402) binding

2uytA Structure of l-rhamnulose kinase in complex with adp and beta-l- rhamnulose. (see paper)
78% identity, 97% coverage: 2:476/488 of query aligns to 1:475/479 of 2uytA

query
sites
2uytA

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M

M

G

G

L

L

W

W

L

L

Q
|
Q

R

R

V

V

L

L

R

Q

E

E

Q

R

N

Q

V

I

S

N

D

D

L
|
L

P

P

A

A

L

L

I

I

A

A

R

A

T

T

A

Q

A

A

L

L

P

P

A

A

C

C

R

R

F

F

V

I

I

I

D

N

C

P

N

N

D

D

R

R

F

F

I

I

N

N

P

P

D

D

D

E

M

M

S

C

A

S

E

E

I

I

Q

Q

A

A

C

C

R

R

E

E

S

M

G

A

Q

Q

P

P

V

I

P

P

D

E

T

S

D

D

A

A

E

E

L

L

A

A

R

R

C

C

I

I

F

F

D

D

S

S

L

L

A

A

L

L

Y

Y

T

A

R

D

V

V

L

L

N

H

E

E

L

L

A

A

A

Q

L

L

R

R

G

G

Q

E

P

D

F

F

S

S

Q

Q

L

L

H

H

I

I

V

V

G

G

G
|
G

G

G

C

C

Q
|
Q

N
|
N

E

T

L

L

N

N

Q

Q

L

L

C

C

A

A

D

D

A

A

C

C

G

G

I

I

T

R

V

V

V

I

A

A

G

G

P

P

V

V

E

E

A

A

S

S

T

T

L

L

G

G

N

N

I

I

G

G

I

I

Q

Q

L

L

M

M

T

T

L

L

D

D

E

E

L

L

N

N

V

V

D

D

E

D

F

F

R

R

Q

Q

V

V

R

S

Q

T

N

T

Y

A

A

N

L

L

T

T

F

F

T

T

P

P

N

N

P

P

E

D

N

S

E

E

I

I

A

A

R

H

F

Y

V

V

A

A

binding adenosine-5'-diphosphate: S13 (= S14), R16 (= R17), G257 (= G258), T258 (= T259), Q303 (= Q304), L314 (= L315), G401 (= G402), Q404 (= Q405), N405 (= N406)
binding 6-deoxy-beta-L-fructofuranose: W81 (= W82), H235 (= H236), D236 (= D237), W259 (= W260)

2cglA Crystal structure of l-rhamnulose kinase from escherichia coli in complex with l-fructose, adp and a modeled atp gamma phosphate. (see paper)
78% identity, 97% coverage: 2:476/488 of query aligns to 1:475/479 of 2cglA

query
sites
2cglA

S

T

L

F

R

R

H

N

C

C

V

V

A

A

V

V

D

D

L

L

G
|
G

A

A

S
|
S

S

S

G

G

R
|
R

V

V

M

M

L

L

A

A

S

R

Y

Y

Q

E

P

R

G

E

Q

C

Q

R

T

S

L

L

A

T

L

L

R

R

E

E

I

I

H

H

R

R

F

F

T

N

N

N

S

G

L

L

Q

H

K

S

V

Q

D

N

G

G

F

Y

D

V

C

T

W

W

D

D

L

V

D

D

S

S

L

L

E

E

G

S

E

A

I

I

R

R

R

L

G

G

L

L

E

N

K

K

V

V

C

C

E

A

Q

A

G

G

I

I

L

A

I

I

D

D

S

S

I

I

G

G

I

I

D

D

T

T

W
|
W

G
|
G

V

V

D

D

Y

F

V

V

L

L

D

D

K

Q

R

Q

G

G

Q

Q

R

R

V

V

G

G

L

L

P

P

V

V

S

A

Y

Y

R

R

D

D

S

S

R

R

T

T

Q

N

G

G

L

L

M

M

R

A

H

Q

A

A

E

Q

A

Q

Q

Q

L

L

G

G

R

K

A

R

E

D

I

I

Y

Y

R

Q

R

R

S

S

G

G

I

I

Q

Q

F

F

L

L

P

P

F

F

N

N

T

T

L

L

Y

Y

Q

Q

L

L

R

R

A

A

L

L

V

T

E

E

Q

Q

P

P

E

E

L

L

V

I

S

P

Q

H

A

I

A

A

H

H

A

A

L

L

I

M

P

P

D

D

Y

Y

F

F

S

S

F

Y

R

R

L

L

T

T

G

G

N

K

M

M

N

N

W

W

E

E

Y

Y

T

T

N

N

A

A

T

T

Q

Q

L

L

V

V

N

N

I

I

N

N

S

S

D

D

S

D

W

W

D

D

E

E

D

S

L

L

N

A

W

W

S

S

G

G

A

A

P

N

R

K

E

A

W

W

F

F

G

G

T

R

P

P

T

T

H

H

P

P

G

G

N

N

V

V

I

I

G

G

H

H

W

W

I

I

C

C

P

P

Q

Q

G

G

N

N

H

E

I

I

P

P

V

V

A

A

V

V

A

A

S

S

H
|
H

D
|
D

T

T

A

A

S

S

A

A

V

V

I

I

A

A

S

S

P

P

L

L

A

N

S

G

K

S

N

R

A

A

Y

Y

L

L

S

S

G
|
G

T
|
T

W
|
W

S

S

L

L

M

M

G

G

F

F

E

E

S

S

K

Q

I

T

P

P

C

F

T

T

S

N

D

D

A

T

A

A

L

L

R

A

A

A

N

N

I

I

T

T

N

N

E

E

G

G

A

A

E

E

G

G

R

R

Y

Y

R

R

V

V

L

L

K

K

N
|
N

I

I

M

M

G

G

L

L

W

W

L

L

Q
|
Q

R

R

V

V

L

L

R

Q

E

E

Q

R

N

Q

V

I

S

N

D

D

L
|
L

P

P

A

A

L

L

I

I

A

A

R

A

T

T

A

Q

A

A

L

L

P

P

A

A

C

C

R

R

F

F

V

I

I

I

D

N

C

P

N

N

D

D

R

R

F

F

I

I

N

N

P

P

D

D

D

E

M

M

S

C

A

S

E

E

I

I

Q

Q

A

A

C

C

R

R

E

E

S

M

G

A

Q

Q

P

P

V

I

P

P

D

E

T

S

D

D

A

A

E

E

L

L

A

A

R

R

C

C

I

I

F

F

D

D

S

S

L

L

A

A

L

L

Y

Y

T

A

R

D

V

V

L

L

N

H

E

E

L

L

A

A

A

Q

L

L

R

R

G

G

Q

E

P

D

F

F

S

S

Q

Q

L

L

H

H

I

I

V

V

G

G

G
|
G

G

G

C

C

Q
|
Q

N
|
N

E

T

L

L

N

N

Q

Q

L

L

C

C

A

A

D

D

A

A

C

C

G

G

I

I

T

R

V

V

V

I

A

A

G

G

P

P

V

V

E

E

A

A

S

S

T

T

L

L

G

G

N

N

I

I

G

G

I

I

Q

Q

L

L

M

M

T

T

L

L

D

D

E

E

L

L

N

N

V

V

D

D

E

D

F

F

R

R

Q

Q

V

V

R

S

Q

T

N

T

Y

A

A

N

L

L

T

T

F

F

T

T

P

P

N

N

P

P

E

D

N

S

E

E

I

I

A

A

R

H

F

Y

V

V

A

A

binding adenosine-5'-diphosphate: S13 (= S14), R16 (= R17), G257 (= G258), T258 (= T259), Q303 (= Q304), L314 (= L315), G401 (= G402), Q404 (= Q405), N405 (= N406)
binding beta-L-fructofuranose: W81 (= W82), G82 (= G83), H235 (= H236), D236 (= D237), W259 (= W260), N295 (= N296)
binding phosphite ion: G11 (= G12), T258 (= T259)

2cgjA Crystal structure of l-rhamnulose kinase from escherichia coli in complex with l-fructose and adp. (see paper)
78% identity, 97% coverage: 2:476/488 of query aligns to 1:475/479 of 2cgjA

query
sites
2cgjA

S

T

L

F

R

R

H

N

C

C

V

V

A

A

V

V

D

D

L

L

G

G

A

A

S
|
S

S

S

G

G

R

R

V

V

M

M

L

L

A

A

S

R

Y

Y

Q

E

P

R

G

E

Q

C

Q

R

T

S

L

L

A

T

L

L

R

R

E

E

I

I

H

H

R

R

F

F

T

N

N

N

S

G

L

L

Q

H

K

S

V

Q

D

N

G

G

F

Y

D

V

C

T

W

W

D

D

L

V

D

D

S

S

L

L

E

E

G

S

E

A

I

I

R

R

R

L

G

G

L

L

E

N

K

K

V

V

C

C

E

A

Q

A

G

G

I

I

L

A

I

I

D

D

S

S

I

I

G

G

I

I

D

D

T

T

W

W

G
|
G

V

V

D

D

Y

F

V

V

L

L

D

D

K

Q

R

Q

G

G

Q

Q

R

R

V

V

G

G

L

L

P

P

V

V

S

A

Y
|
Y

R

R

D

D

S

S

R

R

T

T

Q

N

G

G

L

L

M

M

R

A

H

Q

A

A

E

Q

A

Q

Q

Q

L

L

G

G

R

K

A

R

E

D

I

I

Y

Y

R

Q

R

R

S

S

G

G

I

I

Q

Q

F

F

L

L

P

P

F

F

N

N

T

T

L

L

Y

Y

Q

Q

L

L

R

R

A

A

L

L

V

T

E

E

Q

Q

P

P

E

E

L

L

V

I

S

P

Q

H

A

I

A

A

H

H

A

A

L

L

I

M

P

P

D

D

Y

Y

F

F

S

S

F

Y

R

R

L

L

T

T

G

G

N

K

M

M

N

N

W

W

E

E

Y

Y

T

T

N

N

A

A

T

T

Q

Q

L

L

V

V

N

N

I

I

N

N

S

S

D

D

S

D

W

W

D

D

E

E

D

S

L

L

N

A

W

W

S

S

G

G

A

A

P

N

R

K

E

A

W

W

F

F

G

G

T

R

P

P

T

T

H

H

P

P

G

G

N

N

V

V

I

I

G

G

H

H

W

W

I

I

C

C

P

P

Q

Q

G

G

N

N

H

E

I

I

P

P

V

V

A

A

V

V

A

A

S

S

H
|
H

D
|
D

T
|
T

A

A

S

S

A

A

V

V

I

I

A

A

S

S

P

P

L

L

A

N

S

G

K

S

N

R

A

A

Y

Y

L

L

S

S

G
|
G

T
|
T

W
|
W

S

S

L

L

M

M

G

G

F

F

E

E

S

S

K

Q

I

T

P

P

C

F

T

T

S

N

D

D

A

T

A

A

L

L

R

A

A

A

N

N

I

I

T

T

N

N

E

E

G

G

A

A

E

E

G

G

R

R

Y

Y

R

R

V

V

L

L

K

K

N
|
N

I

I

M

M

G

G

L

L

W

W

L

L

Q
|
Q

R

R

V

V

L

L

R

Q

E

E

Q

R

N

Q

V

I

S

N

D

D

L
|
L

P

P

A

A

L

L

I

I

A

A

R

A

T

T

A

Q

A

A

L

L

P

P

A

A

C

C

R

R

F

F

V

I

I

I

D

N

C

P

N

N

D

D

R

R

F

F

I

I

N

N

P

P

D

D

D

E

M

M

S

C

A

S

E

E

I

I

Q

Q

A

A

C

C

R

R

E

E

S

M

G

A

Q

Q

P

P

V

I

P

P

D

E

T

S

D

D

A

A

E

E

L

L

A

A

R

R

C

C

I

I

F

F

D

D

S

S

L

L

A

A

L

L

Y

Y

T

A

R

D

V

V

L

L

N

H

E

E

L

L

A

A

A

Q

L

L

R

R

G

G

Q

E

P

D

F

F

S

S

Q

Q

L

L

H

H

I

I

V

V

G

G

G
|
G

G

G

C

C

Q
|
Q

N
|
N

E

T

L

L

N

N

Q

Q

L

L

C

C

A

A

D

D

A

A

C

C

G

G

I

I

T

R

V

V

V

I

A

A

G

G

P

P

V

V

E

E

A

A

S

S

T

T

L

L

G

G

N

N

I

I

G

G

I

I

Q

Q

L

L

M

M

T

T

L

L

D

D

E

E

L

L

N

N

V

V

D

D

E

D

F

F

R

R

Q

Q

V

V

R

S

Q

T

N

T

Y

A

A

N

L

L

T

T

F

F

T

T

P

P

N

N

P

P

E

D

N

S

E

E

I

I

A

A

R

H

F

Y

V

V

A

A

binding adenosine-5'-diphosphate: S13 (= S14), G257 (= G258), T258 (= T259), Q303 (= Q304), L314 (= L315), G401 (= G402), Q404 (= Q405), N405 (= N406)
binding beta-L-fructofuranose: G82 (= G83), Y101 (= Y102), H235 (= H236), D236 (= D237), T237 (= T238), W259 (= W260), N295 (= N296)

3ll3B The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
28% identity, 24% coverage: 100:217/488 of query aligns to 95:212/490 of 3ll3B

query
sites
3ll3B

V

I

S

T

Y

W

R

A

D

D

S

N

R

C

T

A

Q

K

G

S

L

I

M

V

R

Q

H

D

A

A

E

K

A

N

Q

R

L

G

G

F

R

A

A

Q

E

Q

I

I

Y

Y

R

R

R

K

S

T

G

G

I

M

Q

P

F

M

L
x
H

P

P

F

M

N

A

T

P

L

I

Y

Y

Q

K

L

L

R

L

A

W

L

L

V

K

E

N

Q

K

P

T

E

E

L

V

V

F

S

S

Q

Q

A

A

A

Q

H

K

A

W

L

I

L

G

I

I

P

K

D

E

Y

Y

F

I

S

I

F

F

R

R

L

L

T

T

G

G

N

K

M

L

N

V

W

T

E

D

Y

T

T

T

N

M

A

A

T

A

T

G

T

T

Q

G

L

I

V

L

N

N

I

L

N

K

S

T

D

L

S

T

W

W

D

D

E

Q

D

E

L

L

N

D

W

I

S

L

G

K

A

I

P

K

R

K

E

E

W

Q

F

L

G

P

T

K

P

I

T

A

H

Q

P

P

G

T

N

K

V

V

I

I

binding 1-deoxy-d-xylulose-5-phosphate: H127 (≠ L132)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 258, 259, 298, 314, 399, 400, 402
binding 1-deoxy-d-xylulose-5-phosphate: 295, 338, 340, 347

3ll3A The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
28% identity, 24% coverage: 100:217/488 of query aligns to 96:213/492 of 3ll3A

query
sites
3ll3A

V

I

S

T

Y

W

R

A

D

D

S

N

R

C

T

A

Q

K

G

S

L

I

M

V

R

Q

H

D

A

A

E

K

A

N

Q

R

L

G

G

F

R

A

A

Q

E

Q

I

I

Y

Y

R

R

R

K

S

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G

G

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M

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F

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L
x
H

P

P

F

M

N

A

T

P

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I

Y

Y

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L

L

R

L

A

W

L

L

V

K

E

N

Q

K

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E

E

L

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V

F

S

S

Q

Q

A

A

A

Q

H

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A

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L

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E

Y

Y

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F

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L

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T

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G

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Y

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T

T

N

M

A

A

T

A

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G

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G

L

I

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L

N

N

I

L

N

K

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D

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W

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D

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E

Q

D

E

L

L

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D

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I

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A

H

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P

G

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N

K

V

V

I

I

binding 1-deoxy-d-xylulose-5-phosphate: H128 (≠ L132)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 259, 260, 299, 316, 320, 400, 401, 402
binding 1-deoxy-d-xylulose-5-phosphate: 296, 342, 349
binding d-xylulose: 78, 79, 80, 238, 343

2w40A Crystal structure of plasmodium falciparum glycerol kinase with bound glycerol (see paper)
25% identity, 26% coverage: 72:196/488 of query aligns to 72:202/501 of 2w40A

query
sites
2w40A

I

V

L

I

I

I

D

K

S

C

I

I

G

G

I

I

D

T

T

N

W

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G
x
R

V
x
E

D

T

Y

V

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I

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K

R

-

I

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G

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x
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E

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H
x
E

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A
|
A

Q

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Y

G

N

R

N

A

N

E

D

I

I

Y

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K

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G

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Y

F

F

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N

P

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F
x
Y

N

F

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S

L

A

Y

F

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K

L

I

R

L

A

W

L

L

V

I

E

Q

Q

N

P

P

E

E

L

I

V

K

S

Q

Q

K

A

I

-

D

A

G

H

T

A

A

L

V

L

I

-

G

-

N

I

I

P

N

D

T

Y

W

F

L

S

I

F

F

R

N

L

L

T

T

-

K

G

G

N

N

M

C

N

Y

W

T

E

D

Y

V

T

T

N

N

A

A

T

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T

R

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L

L

L

V

M

N

D

I

I

N

N

S

T

D

L

S

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W

W

D

D

E

E

D

K

L

M

binding glycerol: R83 (≠ G83), E84 (≠ V84), W103 (≠ Y102), E114 (≠ H113), A117 (= A116), Y135 (≠ F134)

Sites not aligning to the query:

binding glycerol: 246, 271

2w41B Crystal structure of plasmodium falciparum glycerol kinase with adp (see paper)
25% identity, 26% coverage: 72:196/488 of query aligns to 78:208/507 of 2w41B

query
sites
2w41B

I

V

L

I

I

I

D

K

S

C

I

I

G

G

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V

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A

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Y

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T

G

G

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T

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Y

F

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Y

F

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L

I

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L

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N

P

P

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E

L

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K

A

I

-

D

A

G

H

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A

A

L

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L

I

-

G

-

N

I

I

P

N

D

T

Y

W

F

L

S

I

F

F

R

N

L

L

T

T

-

K

G

G

N

N

M

C

N

Y

W

T

E

D

Y

V

T

T

N

N

A

A

T

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T

R

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Q

L

L

L

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M

N

D

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I

N

N

S

T

D

L

S

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W

W

D

D

E

E

D

K

L

M

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 273, 274, 319, 322, 334, 337, 420, 421, 424

5ya2A Crystal structure of lsrk-hpr complex with adp (see paper)
24% identity, 24% coverage: 100:218/488 of query aligns to 94:211/478 of 5ya2A

query
sites
5ya2A

V

V

S

D

Y

A

R

R

D

A

S

A

R

R

T

E

Q

V

G

S

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E

M

L

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A

N

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N

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F

R

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A

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Y

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R

A

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G

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-

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A

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A

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Y

P

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L

L

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L

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H

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A

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A

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Y

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N

A

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I

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G

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 297, 301, 304, 323, 402, 406

5ya1A Crystal structure of lsrk-hpr complex with atp (see paper)
24% identity, 24% coverage: 100:218/488 of query aligns to 94:211/478 of 5ya1A

query
sites
5ya1A

V

V

S

D

Y

A

R

R

D

A

S

A

R

R

T

E

Q

V

G

S

L

E

M

L

R

K

H

E

A

L

E

H

A

N

Q

N

L

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G

F

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E

A

N

E

E

I

V

Y

Y

R

R

R

A

S

T

G

G

I

-

Q

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F

T

L

L

P

A

F

L

N

S

T

A

L

I

Y

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R

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L

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L

A

W

L

L

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A

E

H

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H

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D

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I

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A

A

A

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A

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I

I

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S

D

D

Y

W

F

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A

F

Y

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G

G

N

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M

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N

A

W

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E

D

Y

P

T

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N

N

A

A

T

G

T

T

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G

L

L

V

L

N

D

I

L

N

T

S

T

D

R

S

D

W

W

D

K

E

P

D

A

L

L

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G

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 297, 301, 304, 323, 402, 406

Query Sequence

>BWI76_RS00680 FitnessBrowser__Koxy:BWI76_RS00680
MSLRHCVAVDLGASSGRVMLASYQPGQQTLALREIHRFTNSLQKVDGFDCWDLDSLEGEI
RRGLEKVCEQGILIDSIGIDTWGVDYVLLDKRGQRVGLPVSYRDSRTQGLMRHAEAQLGR
AEIYRRSGIQFLPFNTLYQLRALVEQQPELVSQAAHALLIPDYFSFRLTGNMNWEYTNAT
TTQLVNINSDSWDEDLLNWSGAPREWFGTPTHPGNVIGHWICPQGNHIPVVAVASHDTAS
AVIASPLASKNAAYLSSGTWSLMGFESKIPCTSDAALRANITNEGGAEGRYRVLKNIMGL
WLLQRVLREQNVSDLPALIARTAALPACRFVIDCNDDRFINPDDMSAEIQAACRESGQPV
PDTDAELARCIFDSLALLYTRVLNELAALRGQPFSQLHIVGGGCQNELLNQLCADACGIT
VVAGPVEASTLGNIGIQLMTLDELNNVDEFRQVVRQNYALTTFTPNPENEIARFVAQFQP
QQNKELCA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory