SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS01515 FitnessBrowser__Koxy:BWI76_RS01515 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P69791 PTS system N,N'-diacetylchitobiose-specific EIIA component; EIIA-Chb; EIII-Chb; IIIcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see paper)
47% identity, 96% coverage: 2:102/105 of query aligns to 15:115/116 of P69791

query
sites
P69791

E

E

D

E

L

L

E

E

T

E

I

V

M

M

E

G

L

L

L

I

V

I

N

N

A

S

G

G

S

Q

A

A

R

R

S

S

S

L

A

A

L

Y

T

A

A

A

L

L

Q

K

L

Q

A

A

R

K

K

Q

G

G

D

D

F

F

A

A

E

K

Q

A

A

M

M

M

T

D

E

Q

S

S

H

R

E

M

F

A

V

L

K

N

H

E

A

A

H

H

K

L

I

V

Q

Q

T

T

Q

K

L

L

I

I

G

E

M

G

D

D

E

A

G

G

S

E

G

G

K

K

L

M

P

K

V

V

N

S

L

L

I

V

T

L

V

V

H

H

S

A

Q

Q

D
|
D

H

H

L

L

M

M

N

T

A

S

M

M

V

L

I

A

Q

R

D

E

L

L

A

I

T

T

D

E

M

L

I

I

E

E

L

L

Y

H

R

E

R

K

I

L

P

K

D92 (= D79) mutation to L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation.

P23532 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Lactococcus lactis subsp. lactis (Streptococcus lactis) (see 2 papers)
35% identity, 94% coverage: 2:100/105 of query aligns to 4:102/105 of P23532

query
sites
P23532

E

E

D

E

L

M

E

T

T

L

I

L

I

G

M

F

E

E

L

I

L

V

V

A

N

Y

A

A

G
|
G

S

D

A

A

R

R

S

S

S

K

A

L

L

L

T

E

A

A

L

L

Q

K

L

A

A

A

R

E

K

N

G

G

D

D

F

F

A

A

A

K

A

A

E

D

Q

S

A

L

M

V

T

V

E

E

S

A

H

G

E

S

F

C

V

I

K

A

H

E

A

A

H

H

K

S

I

S

Q

Q

T

T

Q

G

L

M

I

L

G

A

M

R

D

E

E

A

G

S

S

G

G

E

K

E

L

L

P

P

V

Y

N

S

L

V

I

T

T

M

V

M

H

H

S

G

Q

Q

D
|
D

H

H

L

L

M

M

N

T

A

T

M

I

V

L

I

L

Q

K

D

D

L

V

A

I

T

H

D

H

M

L

I

I

E

E

L

L

Y

Y

R

K

R

R

G18 (= G16) mutation to E: No activity.
D81 (= D79) binding

P0A0D6 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Staphylococcus aureus (see 2 papers)
33% identity, 94% coverage: 2:100/105 of query aligns to 4:102/103 of P0A0D6

query
sites
P0A0D6

E

E

D

E

L

V

E

Q

T

L

I

L

I

G

M

F

E

E

L

I

L

V

V

A

N

F

A

A

G

G

S

D

A

A

R

R

S

S

S

K

A

F

L

L

T

E

A

A

L

L

Q

T

L

A

A

A

R

Q

K

A

G

G

D

D

F

F

A

A

A

K

A

A

E

D

Q

A

A

L

M

I

T

E

E

E

S

G

H

N

E

N

F

C

V

I

K

A

H

E

A

A

H

H

K

R

I

A

Q

Q

T

T

Q

S

L

L

I

L

G

A

M

K

D

E

E

A

G

Q

S

G

G

D

K

D

L

I

P

A

V

Y

N

S

L

V

I

T

T

M

V

M

H
|
H

S

G

Q

Q

D

D

H
|
H

L

L

M

M

N

T

A

T

M

I

V

L

I

L

Q

K

D

D

L

L

A

M

T

K

D

H

M

L

I

L

E

E

L

F

Y

Y

R

K

R

R

H78 (= H76) active site, Tele-phosphohistidine intermediate; mutation to S: Abolished activity.
H82 (= H80) mutation to S: Reduced activity.

Query Sequence

>BWI76_RS01515 FitnessBrowser__Koxy:BWI76_RS01515
MEDLETIIMELLVNAGSARSSALTALQLARKGDFAAAEQAMTESHEFVKHAHKIQTQLIG
MDEGSGKLPVNLITVHSQDHLMNAMVIQDLATDMIELYRRIPLAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory