SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS11245 FitnessBrowser__Koxy:BWI76_RS11245 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
27% identity, 68% coverage: 10:187/261 of query aligns to 19:202/281 of P94530

query
sites
P94530

F

F

M

M

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M

A

L

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A

L

Y

Y

L

L

Y

F

I

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P

P

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L

L

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G

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K

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E

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R

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D

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D

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A

D180 (≠ L165) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
27% identity, 55% coverage: 62:204/261 of query aligns to 87:234/296 of P68183

query
sites
P68183

H

N

S

S

L

V

T

K

M

V

A

A

V

G

L

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S

A

A

T

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F

G

A

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C

A

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A

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Y

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M

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R

P

G

G

K

K

P

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K

M

G

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F

L

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M

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P

P

D

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H

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G

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G

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P

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S

E190 (= E160) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A162) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G166) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P179) mutation to A: No effect.

Query Sequence

>BWI76_RS11245 FitnessBrowser__Koxy:BWI76_RS11245
MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTTNWYSLLMNNDSLLQAA
QHSLTMAVLSATFATLIGSLTAVALYRYRFRGKPFVSGMLFVVMMSPDIVMAISLLVLFM
LIGIQLGFWSLLFSHITFCLPFVVVTVFSRLKGFDVRMLEAAKDLGASELTILRKIILPL
ALPAVAAGWLLSFTLSMDDVVVSSFVTGPGYEILPLKIYSMVKVGVSPEVNALATILLVL
SLVMVIASQLIARDKTQGTLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory