SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS14445 FitnessBrowser__Koxy:BWI76_RS14445 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8eyzA Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
53% identity, 90% coverage: 25:250/250 of query aligns to 1:226/226 of 8eyzA

query
sites
8eyzA

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binding glutamine: D10 (= D34), F13 (= F37), F50 (= F74), A67 (= A91), G68 (= G92), T70 (= T94), R75 (= R99), K115 (= K139), T118 (= T142), G119 (≠ A143), H156 (= H179), D157 (= D180)
binding azidobis[N~2~,N~3~-dihydroxybut-2-ene-2,3-diaminato(2-)-kappa~2~N,N']cobalt: C72 (≠ T96), D73 (= D97), E74 (≠ A98), G119 (≠ A143), D122 (≠ I146), E181 (≠ L206)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
48% identity, 87% coverage: 29:245/250 of query aligns to 6:225/226 of 4zv1A

query
sites
4zv1A

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binding arginine: E11 (≠ D34), F14 (= F37), F52 (= F74), A69 (= A91), G70 (= G92), M71 (≠ I93), T72 (= T94), R77 (= R99), Q117 (≠ K139), S120 (≠ T142), T121 (≠ A143), D161 (= D180)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
48% identity, 88% coverage: 29:247/250 of query aligns to 6:225/225 of 4zv2A

query
sites
4zv2A

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binding glutamine: E11 (≠ D34), F14 (= F37), F52 (= F74), A69 (= A91), G70 (= G92), M71 (≠ I93), T72 (= T94), R77 (= R99), S120 (≠ T142), T121 (≠ A143), D159 (= D180)

4g4pA Crystal structure of glutamine-binding protein from enterococcus faecalis at 1.5 a (see paper)
41% identity, 88% coverage: 21:240/250 of query aligns to 8:229/235 of 4g4pA

query
sites
4g4pA

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binding glutamine: D21 (= D34), F24 (= F37), F62 (= F74), A79 (= A91), G80 (= G92), M81 (≠ I93), S82 (≠ T94), R87 (= R99), K127 (= K139), T130 (= T142), E131 (≠ A143), D171 (= D180)

2pvuA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
38% identity, 89% coverage: 28:250/250 of query aligns to 6:229/235 of 2pvuA

query
sites
2pvuA

K

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binding lysine: D12 (= D34), F15 (= F37), W52 (≠ F74), S69 (≠ A91), G70 (= G92), I71 (= I93), T72 (= T94), R77 (= R99), Q116 (≠ K139), T119 (= T142), T120 (vs. gap), D159 (= D180)

2q2cA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
38% identity, 89% coverage: 28:250/250 of query aligns to 2:225/231 of 2q2cA

query
sites
2q2cA

K

K

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A

I

V

A

M

K

K

K

A

M

A

N

G

V

L

S

D

Y

Y

E

E

L

L

R

K

P

N

M

I

D

G

F
x
W

S

D

G

P

L

L

I

F

P

A

G

S

L

L

Q

Q

S

S

R

K

N

E

L

V

D

D

V

M

A

G

M

I

A
x
S

G
|
G

I
|
I

T
|
T

I

I

T

T

D

D

A

E

R
|
R

K

K

Q

Q

V

S

V

Y

D

D

F

F

S

S

D

D

G

P

Y

Y

Y

F

D

E

A

A

D

T

L

Q

L

V

M

I

A

L

V

V

K

K

S

Q

G

G

D

-

D

S

S

P

I

V

T

K

K

N

F

A

S

L

E

D

L

L

A

K

G

G

K

K

K

T

V

I

G

G

L

V

K
x
Q

Q

N

G

A

T
|
T

-
x
T

-

G

-

Q

-

E

-

A

A

A

A

E

A

K

I

L

F

F

M

G

K

K

S

G

K

P

Y

H

K

-

A

-

N

-

Y

I

V

K

E

K

F

F

P

E

N

T

I

T

D

V

N

V

A

A

Y

I

L

M

D

E

L

L

Q

L

A

N

G

G

N

G

L

V

D

D

A

A

V

V

V

I

H

T

D
|
D

S

N

P

A

N

V

V

A

L

N

Y

E

Y

Y

V

V

K

K

T

N

A

N

G

P

G

N

G

K

K

K

V

L

K

Q

S

V

T

I

G

E

E

D

T

P

D

K

S

N

I

F

L

A

P

S

Q

E

K

Y

Y

Y

G

G

F

M

A

I

L

F

Q

P

K

K

N

N

S

S

S

E

L

L

T

K

P

A

K

K

V

V

D

D

A

E

A

A

L

L

N

K

A

N

L

V

R

I

A

N

D

S

G

G

T

K

Y

Y

D

T

K

E

I

I

Y

Y

F

K

K

K

W

W

F

F

N

G

K

K

Q

E

P

P

K

K

binding histidine: F11 (= F37), W48 (≠ F74), S65 (≠ A91), G66 (= G92), I67 (= I93), T68 (= T94), R73 (= R99), Q112 (≠ K139), T115 (= T142), T116 (vs. gap), D155 (= D180)

2q2aA Crystal structures of the arginine-, lysine-, histidine-binding protein artj from the thermophilic bacterium geobacillus stearothermophilus (see paper)
38% identity, 89% coverage: 28:250/250 of query aligns to 12:235/241 of 2q2aA

query
sites
2q2aA

K

K

I

V

L

V

V

V

G

G

V

T

D
|
D

T

A

A

A

F
|
F

V

A

P

P

F

F

E
|
E

F

Y

K

M

Q

Q

G

K

N

G

K

K

Y

I

V

V

G

G

F

F

D

D

I

V

D

D

L

L

W

L

Q

D

A

A

I

V

A

M

K

K

K

A

M

A

N

G

V

L

S

D

Y

Y

E

E

L

L

R

K

P

N

M

I

D

G

F
x
W

S

D

G

P

L

L

I

F

P

A

G

S

L

L

Q

Q

S

S

R

K

N

E

L

V

D

D

V

M

A

G

M

I

A
x
S

G
|
G

I
|
I

T
|
T

I

I

T

T

D

D

A

E

R
|
R

K

K

Q

Q

V

S

V

Y

D

D

F

F

S

S

D

D

G

P

Y

Y

Y

F

D

E

A

A

D

T

L

Q

L

V

M

I

A

L

V

V

K

K

S

Q

G

G

D

-

D

S

S

P

I

V

T

K

K

N

F

A

S

L

E

D

L

L

A

K

G

G

K

K

K

T

V

I

G

G

L

V

K
x
Q

Q

N

G

A

T
|
T

-
x
T

-

G

-

Q

-

E

-

A

A

A

A

E

A

K

I

L

F

F

M

G

K

K

S

G

K

P

Y

H

K

-

A

-

N

-

Y

I

V

K

E

K

F

F

P

E

N

T

I

T

D

V

N

V

A

A

Y

I

L

M

D

E

L

L

Q

L

A

N

G

G

N

G

L

V

D

D

A

A

V

V

V

I

H

T

D
|
D

S

N

P

A

N

V

V

A

L

N

Y

E

Y

Y

V

V

K

K

T

N

A

N

G

P

G

N

G

K

K

K

V

L

K

Q

S

V

T

I

G

E

E

D

T

P

D

K

S

N

I

F

L

A

P

S

Q

E

K

Y

Y

Y

G

G

F

M

A

I

L

F

Q

P

K

K

N

N

S

S

S

E

L

L

T

K

P

A

K

K

V

V

D

D

A

E

A

A

L

L

N

K

A

N

L

V

R

I

A

N

D

S

G

G

T

K

Y

Y

D

T

K

E

I

I

Y

Y

F

K

K

K

W

W

F

F

N

G

K

K

Q

E

P

P

K

K

binding arginine: D18 (= D34), F21 (= F37), E25 (= E41), W58 (≠ F74), S75 (≠ A91), G76 (= G92), I77 (= I93), T78 (= T94), R83 (= R99), Q122 (≠ K139), T125 (= T142), T126 (vs. gap), D165 (= D180)

4ymxA Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
35% identity, 88% coverage: 29:247/250 of query aligns to 3:224/224 of 4ymxA

query
sites
4ymxA

I

I

L

V

V

M

G

G

V

T

D
x
S

T

A

A
x
D

F
|
F

V

P

P

P

F

F

E
|
E

F

F

K

H

Q

K

-

V

-

E

-

G

-

G

G

K

N

D

K

E

Y

I

V

V

G

G

F

F

D

D

I

I

D

D

L

I

W

A

Q

N

A

A

I

I

A

A

K

K

K

K

M

L

N

G

V

V

S

K

Y

L

E

E

L

I

R

K

P

D

M

M

D

D

F
|
F

S

K

G

G

L

L

I

I

P

P

G

A

L

L

Q

Q

S

A

R

G

N

R

L

V

D

D

V

M

A

V

M

I

A
|
A

G
|
G

I
x
M

T
|
T

I

P

T

T

D

A

A

E

R
|
R

K

K

Q

K

V

S

V

V

D

D

F

F

S

S

D

D

G

L

Y

Y

Y

Y

D

D

A

S

D

R

L

Q

L

V

M

V

A

V

V

V

K

K

S

N

G

-

D

D

D

S

S

P

I

I

T

S

K

K

F

F

S

D

E

D

L

L

A

K

G

V

K

K

K

T

V

I

G

A

L

V

K
x
Q

Q

I

G

G

T
|
T

A
x
T

A

S

A

E

I

E

F

A

M

A

K

K

S

K

K

I

Y

P

K

N

A

V

N

K

Y

L

V

K

E

Q

F

L

P

N

N

R

I

V

D

S

N

D

A

E

Y

F

L

M

D

D

L

L

Q

Q

A

N

G

G

N

R

L

C

D

D

A

A

V

I

V

V

H

V

D
x
E

S

D

P

T

N

V

V

A

L

K

Y

A

Y

Y

V

L

K

K

T

E

A

Y

G

K

G

D

G

M

K

K

V

I

K

L

S

Y

T

M

G

D

E

E

T

I

D

N

S

N

I

V

L

E

P

N

Q

G

K

S

Y

A

G

V

F

A

A

V

L

A

Q

K

K

G

N

N

S

K

S

S

L

L

T

L

P

D

K

V

V

V

D

N

A

E

A

V

L

I

N

K

A

E

L

L

R

K

A

Q

D

S

G

G

T

E

Y

Y

D

D

K

K

I

L

Y

V

F

D

K

K

W

W

F

F

N

K

K

Q

binding arginine: S8 (≠ D34), D10 (≠ A36), F11 (= F37), E15 (= E41), F52 (= F74), A69 (= A91), G70 (= G92), M71 (≠ I93), T72 (= T94), R77 (= R99), Q116 (≠ K139), T119 (= T142), T120 (≠ A143), E157 (≠ D180)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
39% identity, 89% coverage: 23:245/250 of query aligns to 6:228/229 of 5t0wA

query
sites
5t0wA

V

I

M

M

A

K

Q

R

D

G

K

T

I

L

L

R

V

V

G

G

V

T

D
|
D

T

A

A

D

F
x
Y

V

K

P

P

F

F

E

S

F

F

K

K

Q

D

G

K

N

N

-

G

K

Q

Y

Y

V

T

G

G

F

F

D

D

I

I

D

D

L

L

W

A

Q

K

A

A

I

L

A

A

K

K

K

E

M

L

N

G

V

V

S

K

Y

V

E

E

L

F

R

V

P

P

M

T

D

T

F
x
W

S

D

G

G

L

I

I

I

P

P

G

A

L

L

Q

Q

S

T

R

G

N

K

L

F

D

D

V

I

A

V

M

M

A
x
S

G
|
G

I
x
M

T
|
T

I

I

T

T

D

P

A

E

R
|
R

K

K

Q

K

V

K

V

V

D

D

F

F

S

S

D

D

G

P

Y

Y

Y

M

D

T

A

A

D

G

L

Q

L

T

M

I

A

L

V

V

K

K

S

K

G

D

D

N

-

A

D

D

S

K

I

I

T

K

K

S

F

F

S

E

E

D

L

L

A

N

G

K

K

P

-

D

-

V

K

K

V

V

G

A

L

V

K
x
Q

Q

L

G

G

T
|
T

-
x
T

-

S

-

E

-

Q

A

A

A

A

A

K

I

E

F

F

M

L

K

P

S

-

K

-

Y

-

K

K

A

A

N

K

Y

I

V

R

E

T

F

F

P

E

N

N

I

N

D

A

N

E

A

A

Y

F

L

Q

D

E

L

V

Q

V

A

S

G

G

N

R

L

A

D

D

A

A

V

M

V

V

H

T

D
|
D

S

S

P

P

N

V

V

A

L

A

Y

Y

Y

Y

V

A

K

K

T

L

A

A

G

-

G

-

G

-

K

-

V

V

K

V

S

V

T

V

G

D

E

E

T

P

D

F

S

T

I

H

L

E

P

P

Q

-

K

-

Y

L

G

G

F

F

A

A

L

I

Q

R

K

K

-

G

N

D

S

P

S

E

L

L

T

L

P

N

K

W

V

V

D

N

A

N

A

W

L

L

N

K

A

Q

L

M

R

K

A

K

D

D

G

G

T

T

Y

Y

D

D

K

K

I

L

Y

Y

F

E

K

K

W

W

F

F

binding arginine: D17 (= D34), Y20 (≠ F37), W58 (≠ F74), S75 (≠ A91), G76 (= G92), M77 (≠ I93), T78 (= T94), R83 (= R99), Q126 (≠ K139), T129 (= T142), T130 (vs. gap), D168 (= D180)

6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
38% identity, 88% coverage: 29:247/250 of query aligns to 12:229/229 of 6svfA

query
sites
6svfA

I

L

L

L

V

V

G

G

V

L

D
x
S

T

A

A
x
D

F
|
F

V

P

P

P

F

F

E
|
E

F

F

K

V

Q

D

G

E

N

N

-

G

K

N

Y

I

V

V

G

G

F

F

D

D

I

V

D

D

L

L

W

A

Q

K

A

E

I

I

A

A

K

R

K

R

M

L

N

G

V

V

S

E

Y

L

E

K

L

I

R

V

P

D

M

M

D

T

F
|
F

S

D

G

G

L

L

I

I

P

P

G

S

L

L

Q

L

S

T

R

K

N

K

L

I

D

D

V

V

A

I

M

I

A
x
S

G
|
G

I
x
M

T
|
T

I

I

T

T

D

E

A

E

R
|
R

K

K

Q

K

V

V

V

V

D

A

F

F

S

S

D

D

G

P

Y

Y

Y

F

D

D

A

A

D

G

L

Q

L

V

M

I

A

V

V

V

K

R

S

K

G

D

D

S

D

D

S

F

I

R

T

P

K

K

-

T

F

Y

S

E

E

D

L

L

A

V

G

G

K

K

K

T

V

V

G

A

L

V

K
x
Q

Q

I

G

G

T
|
T

A
x
T

A

G

A

D

I

I

F

E

M

V

K

-

S

S

K

K

Y

Y

K

D

A

G

-

I

N

K

Y

V

V

V

E

R

F

F

P

D

N

K

I

F

D

T

N

D

A

A

Y

F

L

L

D

E

L

L

Q

K

A

R

G

G

N

R

L

A

D

D

A

A

V

V

V

V

H

L

D
|
D

S

S

P

A

N

T

V

A

L

R

Y

A

Y

F

V

V

K

-

T

A

A

K

G

N

G

P

G

D

K

L

V

V

K

I

S

S

T

S

G

G

E

V

T

L

D

S

S

S

I

-

L

-

P

-

Q

E

K

Q

Y

Y

G

G

F

I

A

A

L

V

Q

R

K

K

-

E

N

D

S

T

S

D

L

L

T

L

P

E

K

F

V

I

D

N

A

S

A

V

L

L

N

R

A

E

L

L

R

K

A

K

D

S

G

G

T

K

Y

Y

D

D

K

V

I

L

Y

I

F

E

K

K

W

W

F

F

N

S

K

E

binding arginine: S17 (≠ D34), D19 (≠ A36), F20 (= F37), E24 (= E41), F58 (= F74), S75 (≠ A91), G76 (= G92), M77 (≠ I93), T78 (= T94), R83 (= R99), Q124 (≠ K139), T127 (= T142), T128 (≠ A143), D165 (= D180)

4kqpA Crystal structure of lactococcus lactis glnp substrate binding domain 2 (sbd2) in complex with glutamine at 0.95 a resolution (see paper)
34% identity, 90% coverage: 22:245/250 of query aligns to 1:226/230 of 4kqpA

query
sites
4kqpA

A

A

V

T

M

P

A

K

Q

K

D

D

K

V

I

Y

L

T

V

I

G

A

V

S

D
|
D

T

N

A

S

F
|
F

V

A

P

P

F

F

E

E

F

F

K

Q

Q

N

G

D

N

D

K

K

-

Q

Y

F

V

T

G

G

F

I

D

D

I

V

D

D

L

L

W

L

Q

N

A

A

I

I

A

A

K

K

K

N

M

Q

N

G

V

F

S

K

Y

L

E

K

L

W

R

N

P

F

M

I

D

G

F
|
F

S

Q

G

A

L

A

I

V

P

D

G

S

L

V

Q

Q

S

S

R

G

N

H

L

A

D

D

V

G

A

M

M

M

A
x
S

G
|
G

I
x
M

T
x
S

I

I

T

T

D

D

A

A

R
|
R

K

K

Q

Q

V

V

V

F

D

D

F

Y

S

G

D

S

G

P

Y

Y

Y

Y

D

S

A

S

D

N

L

L

L

T

M

I

A

A

V

T

K

S

S

S

G

T

D

D

D

D

S

S

I

I

T

K

K

S

F

W

S

K

E

D

L

L

A

K

G

G

K

K

K

T

V

L

G

G

L

A

K
|
K

Q

N

G

G

T
|
T

A
|
A

A

S

A

F

I

D

F

Y

M

L

K

N

S

A

-

H

-

A

-

K

K

E

Y

Y

K

G

A

Y

N

T

Y

V

V

K

E

T

F

F

P

T

N

D

I

A

D

T

N

T

A

M

Y

Y

L

S

D

S

L

L

Q

N

A

N

G

G

N

S

L

I

D

N

A

A

V

L

V

M

H

D

D
|
D

S

E

P

P

N

V

V

I

L

K

Y

Y

Y

A

V

I

K

K

T

Q

A

G

G

-

G

-

G

-

K

-

V

Q

K

K

S

F

T

A

G

T

E

P

T

I

D

K

S

P

I

I

L

P

P

D

Q

G

K

Q

Y

Y

G

G

F

F

A

A

L

V

Q

K

K

K

-

G

-

S

N

N

S

P

S

E

L

L

T

I

P

E

K

M

V

F

D

N

A

N

A

G

L

L

N

A

A

N

L

L

R

R

A

A

D

N

G

G

T

E

Y

Y

D

D

K

K

I

I

Y

I

F

D

K

K

W

Y

F

L

binding glutamine: D13 (= D34), F16 (= F37), F54 (= F74), S71 (≠ A91), G72 (= G92), M73 (≠ I93), S74 (≠ T94), R79 (= R99), K119 (= K139), T122 (= T142), A123 (= A143), D163 (= D180)

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
34% identity, 87% coverage: 29:246/250 of query aligns to 3:229/235 of 5owfA

query
sites
5owfA

I

V

L

R

V

I

G

G

V

T

D

D

T

T

A

T

F
x
Y

V

A

P

P

F

F

E

S

F

S

K

K

Q

D

G

A

-

K

N

G

K

E

Y

F

V

I

G

G

F

F

D

D

I

I

D

D

L

L

W

G

Q

N

A

E

I

M

A

C

K

K

K

R

M

M

N

Q

V

V

S

K

Y

C

E

T

L

W

R

V

P

A

M

S

D

D

F
|
F

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

I

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

D

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

S

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

I

V

A

K

A

S

K

G

G

D

S

D

P

S

I

I

Q

T

P

K

T

F

L

S

E

E

S

L

L

A

K

G

G

K

K

K

H

V

V

G

G

L

V

K
|
K

Q

Q

G

G

T
x
S

A
x
T

A
x
Q

A

E

I

A

F

Y

M

A

K

N

S

D

K

N

Y

W

K

R

A

T

-

K

-

G

-

V

N

D

Y

V

V

V

E

A

F

Y

P

A

N

N

I

Q

D

D

N

L

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

L

D

D

A

A

V

A

V

L

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

A

G

G

G

K

K

E

V

Y

K

A

S

F

T

A

G

G

E

P

T

-

D

-

S

S

I

V

L

K

P

D

Q

K

K

K

Y

Y

-

F

-

G

-

D

-

G

-

T

G

G

F

V

A

G

L

L

Q

R

K

K

-

D

N

D

S

T

S

E

L

L

T

K

P

A

K

A

V

F

D

D

A

K

A

A

L

L

N

T

A

E

L

L

R

R

A

Q

D

D

G

G

T

T

Y

Y

D

D

K

K

I

M

Y

A

F

K

K

K

W

Y

F

F

N

D

binding glutamine: Y11 (≠ F37), F49 (= F74), S66 (≠ A91), S67 (≠ G92), L68 (≠ I93), S69 (≠ T94), R74 (= R99), K114 (= K139), S117 (≠ T142), T118 (≠ A143), Q119 (≠ A144), D158 (= D180)

4zefA Crystal structure of substrate binding domain 2 (sbd2) of abc transporter glnpq from enterococcus faecalis
35% identity, 86% coverage: 26:240/250 of query aligns to 15:231/239 of 4zefA

query
sites
4zefA

Q

K

D

E

K

K

I

Y

L

V

V

I

G

A

V

S

D
|
D

T

S

A

T

F
|
F

V

A

P

P

F

F

E

E

F

F

K

Q

-

N

-

A

Q

Q

G

G

N

D

K

-

Y

Y

V

V

G

G

F

I

D

D

I

V

D

D

L

L

W

V

Q

K

A

R

I

A

A

A

K

E

K

L

M

Q

N

G

V

F

S

T

Y

V

E

E

L

F

R

K

P

F

M

I

D

G

F
|
F

S

S

G

S

L

A

I

V

P

Q

G

A

L

V

Q

E

S

S

R

G

N

Q

L

A

D

D

V

G

A

M

M

V

A
|
A

G
|
G

I
x
M

T
|
T

I

I

T

T

D

D

A

D

R
|
R

K

K

Q

K

V

A

V

F

D

D

F

F

S

S

D

V

G

P

Y

Y

Y

F

D

D

A

S

D

G

L

I

L

Q

M

I

A

A

V

V

K

K

S

K

G

G

D

N

D

D

S

K

I

I

T

K

K

S

F

Y

S

D

E

D

L

L

A

K

G

G

K

K

K

K

V

V

G

G

L

V

K
|
K

Q

I

G

G

T
|
T

A
x
E

A

S

A

A

I

D

F

F

M

L

-

E

-

K

-

N

K

K

S

K

K

K

Y

Y

K

D

A

Y

N

S

Y

I

V

K

E

Y

F

L

P

D

N

T

I

T

D

D

N

A

A

L

Y

Y

L

S

D

A

L

L

Q

E

A

I

G

G

N

E

L

V

D

D

A

A

V

M

V

M

H

D

D
|
D

S

Y

P

P

N

V

V

I

L

G

Y

Y

Y

-

V

-

K

-

T

-

A

-

G

-

G

-

G

-

K

G

V

V

K

A

S

Q

T

N

G

Q

E

P

T

L

D

A

S

T

I

P

L

I

P

P

Q

R

K

E

-

K

-

G

-

G

-

S

Y

Y

G

G

F

F

A

A

L

V

Q

K

K

K

-

G

-

Q

N

N

S

P

S

E

L

L

T

L

P

E

K

M

V

F

D

N

A

E

A

G

L

L

N

K

A

E

L

M

R

K

A

R

D

T

G

G

T

E

Y

Y

D

D

K

K

I

I

binding glutamine: D23 (= D34), F26 (= F37), F64 (= F74), A81 (= A91), G82 (= G92), M83 (≠ I93), T84 (= T94), R89 (= R99), K129 (= K139), T132 (= T142), E133 (≠ A143), D173 (= D180)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
33% identity, 87% coverage: 29:246/250 of query aligns to 6:232/238 of 1lstA

query
sites
1lstA

I

V

L

R

V

I

G

G

V

T

D
|
D

T

T

A

T

F
x
Y

V

A

P

P

F

F

E

S

F

S

K

K

Q

D

G

A

-

K

N

G

K

E

Y

F

V

I

G

G

F

F

D

D

I

I

D

D

L

L

W

G

Q

N

A

E

I

M

A

C

K

K

K

R

M

M

N

Q

V

V

S

K

Y

C

E

T

L

W

R

V

P

A

M

S

D

D

F
|
F

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

I

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

D

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

S

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

I

V

A

K

A

S

K

G

G

D

S

D

P

S

I

I

Q

T

P

K

T

F

L

S

E

E

S

L

L

A

K

G

G

K

K

K

H

V

V

G

G

L

V

K
x
L

Q

Q

G

G

T
x
S

A
x
T

A
x
Q

A

E

I

A

F

Y

M

A

K

N

S

D

K

N

Y

W

K

R

A

T

-

K

-

G

-

V

N

D

Y

V

V

V

E

A

F

Y

P

A

N

N

I

Q

D

D

N

L

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

L

D

D

A

A

V

A

V

L

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

A

G

G

G

K

K

E

V

Y

K

A

S

F

T

A

G

G

E

P

T

-

D

-

S

S

I

V

L

K

P

D

Q

K

K

K

Y

Y

-

F

-

G

-

D

-

G

-

T

G

G

F

V

A

G

L

L

Q

R

K

K

-

D

N

D

S

T

S

E

L

L

T

K

P

A

K

A

V

F

D

D

A

K

A

A

L

L

N

T

A

E

L

L

R

R

A

Q

D

D

G

G

T

T

Y

Y

D

D

K

K

I

M

Y

A

F

K

K

K

W

Y

F

F

N

D

binding lysine: D11 (= D34), Y14 (≠ F37), F52 (= F74), S69 (≠ A91), S70 (≠ G92), L71 (≠ I93), S72 (≠ T94), R77 (= R99), L117 (≠ K139), S120 (≠ T142), T121 (≠ A143), Q122 (≠ A144), D161 (= D180)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
33% identity, 87% coverage: 29:246/250 of query aligns to 6:232/238 of 1lahE

query
sites
1lahE

I

V

L

R

V

I

G

G

V

T

D
|
D

T

T

A

T

F
x
Y

V

A

P

P

F

F

E

S

F

S

K

K

Q

D

G

A

-

K

N

G

K

E

Y

F

V

I

G

G

F

F

D

D

I

I

D

D

L

L

W

G

Q

N

A

E

I

M

A

C

K

K

K

R

M

M

N

Q

V

V

S

K

Y

C

E

T

L

W

R

V

P

A

M

S

D

D

F
|
F

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

I

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

D

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

S

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

I

V

A

K

A

S

K

G

G

D

S

D

P

S

I

I

Q

T

P

K

T

F

L

S

E

E

S

L

L

A

K

G

G

K

K

K

H

V

V

G

G

L

V

K

L

Q

Q

G

G

T
x
S

A
x
T

A
x
Q

A

E

I

A

F

Y

M

A

K

N

S

D

K

N

Y

W

K

R

A

T

-

K

-

G

-

V

N

D

Y

V

V

V

E

A

F

Y

P

A

N

N

I

Q

D

D

N

L

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

L

D

D

A

A

V

A

V

L

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

A

G

G

G

K

K

E

V

Y

K

A

S

F

T

A

G

G

E

P

T

-

D

-

S

S

I

V

L

K

P

D

Q

K

K

K

Y

Y

-

F

-

G

-

D

-

G

-

T

G

G

F

V

A

G

L

L

Q

R

K

K

-

D

N

D

S

T

S

E

L

L

T

K

P

A

K

A

V

F

D

D

A

K

A

A

L

L

N

T

A

E

L

L

R

R

A

Q

D

D

G

G

T

T

Y

Y

D

D

K

K

I

M

Y

A

F

K

K

K

W

Y

F

F

N

D

binding L-ornithine: D11 (= D34), Y14 (≠ F37), F52 (= F74), S69 (≠ A91), S70 (≠ G92), L71 (≠ I93), S72 (≠ T94), R77 (= R99), S120 (≠ T142), T121 (≠ A143), Q122 (≠ A144), D161 (= D180)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
33% identity, 87% coverage: 29:246/250 of query aligns to 6:232/238 of 1lagE

query
sites
1lagE

I

V

L

R

V

I

G

G

V

T

D

D

T

T

A

T

F
x
Y

V

A

P

P

F

F

E

S

F

S

K

K

Q

D

G

A

-

K

N

G

K

E

Y

F

V

I

G

G

F

F

D

D

I

I

D

D

L

L

W

G

Q

N

A

E

I

M

A

C

K

K

K

R

M

M

N

Q

V

V

S

K

Y

C

E

T

L

W

R

V

P

A

M

S

D

D

F
|
F

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

I

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

D

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

S

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

I

V

A

K

A

S

K

G

G

D

S

D

P

S

I

I

Q

T

P

K

T

F

L

S

E

E

S

L

L

A

K

G

G

K

K

K

H

V

V

G

G

L

V

K
x
L

Q

Q

G

G

T
x
S

A
x
T

A
x
Q

A

E

I

A

F

Y

M

A

K

N

S

D

K

N

Y

W

K

R

A

T

-

K

-

G

-

V

N

D

Y

V

V

V

E

A

F

Y

P

A

N

N

I

Q

D

D

N

L

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

L

D

D

A

A

V

A

V

L

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

A

G

G

G

K

K

E

V

Y

K

A

S

F

T

A

G

G

E

P

T

-

D

-

S

S

I

V

L

K

P

D

Q

K

K

K

Y

Y

-

F

-

G

-

D

-

G

-

T

G

G

F

V

A

G

L

L

Q

R

K

K

-

D

N

D

S

T

S

E

L

L

T

K

P

A

K

A

V

F

D

D

A

K

A

A

L

L

N

T

A

E

L

L

R

R

A

Q

D

D

G

G

T

T

Y

Y

D

D

K

K

I

M

Y

A

F

K

K

K

W

Y

F

F

N

D

binding histidine: Y14 (≠ F37), F52 (= F74), S69 (≠ A91), S70 (≠ G92), L71 (≠ I93), S72 (≠ T94), R77 (= R99), L117 (≠ K139), S120 (≠ T142), T121 (≠ A143), Q122 (≠ A144), D161 (= D180)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
33% identity, 87% coverage: 29:246/250 of query aligns to 6:232/238 of 1lafE

query
sites
1lafE

I

V

L

R

V

I

G

G

V

T

D
|
D

T

T

A

T

F
x
Y

V

A

P

P

F

F

E

S

F

S

K

K

Q

D

G

A

-

K

N

G

K

E

Y

F

V

I

G

G

F

F

D

D

I

I

D

D

L

L

W

G

Q

N

A

E

I

M

A

C

K

K

K

R

M

M

N

Q

V

V

S

K

Y

C

E

T

L

W

R

V

P

A

M

S

D

D

F
|
F

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

I

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

D

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

S

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

I

V

A

K

A

S

K

G

G

D

S

D

P

S

I

I

Q

T

P

K

T

F

L

S

E

E

S

L

L

A

K

G

G

K

K

K

H

V

V

G

G

L

V

K
x
L

Q

Q

G

G

T
x
S

A
x
T

A
x
Q

A

E

I

A

F

Y

M

A

K

N

S

D

K

N

Y

W

K

R

A

T

-

K

-

G

-

V

N

D

Y

V

V

V

E

A

F

Y

P

A

N

N

I

Q

D

D

N

L

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

L

D

D

A

A

V

A

V

L

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

A

G

G

G

K

K

E

V

Y

K

A

S

F

T

A

G

G

E

P

T

-

D

-

S

S

I

V

L

K

P

D

Q

K

K

K

Y

Y

-

F

-

G

-

D

-

G

-

T

G

G

F

V

A

G

L

L

Q

R

K

K

-

D

N

D

S

T

S

E

L

L

T

K

P

A

K

A

V

F

D

D

A

K

A

A

L

L

N

T

A

E

L

L

R

R

A

Q

D

D

G

G

T

T

Y

Y

D

D

K

K

I

M

Y

A

F

K

K

K

W

Y

F

F

N

D

binding arginine: D11 (= D34), Y14 (≠ F37), F52 (= F74), S69 (≠ A91), S70 (≠ G92), L71 (≠ I93), S72 (≠ T94), R77 (= R99), L117 (≠ K139), S120 (≠ T142), T121 (≠ A143), Q122 (≠ A144), D161 (= D180)

6fxgB Crystal structure of substrate binding domain 1 (sbd1) of abc transporter glnpq in complex with asparagine
32% identity, 86% coverage: 29:244/250 of query aligns to 5:222/226 of 6fxgB

query
sites
6fxgB

I

V

L

K

V

I

G

A

V

S

D
|
D

T

S

A

S

F
x
Y

V

A

P

P

F

F

E

E

F

F

K

Q

Q

N

G

G

N

Q

K

K

-

K

Y

W

V

V

G

G

F

I

D

D

I

V

D

D

L

I

W

M

Q

Q

A

E

I

V

A

A

K

K

K

I

M

N

N

D

V

W

S

K

Y

L

E

E

L

M

R

S

P

Y

M

P

D

G

F
|
F

S

D

G

A

L

A

I

L

P

Q

G

N

L

L

Q

K

S

A

R

G

N

Q

L

V

D

D

V

G

A

I

M

I

A
|
A

G
|
G

I
x
M

T
|
T

I

I

T

T

D

D

A

E

R
|
R

K

K

Q

E

V

T

V

F

D

D

F

F

S

S

D

N

G

P

Y

Y

Y

Y

D

T

A

S

D

A

L

L

L

T

M

I

A

A

V

T

K

-

S

T

G

K

D

D

D

S

S

K

I

L

T

S

K

D

F

Y

S

S

E

D

L

L

A

K

G

G

K

K

K

A

V

V

G

G

L

A

K
|
K

Q

N

G

G

T
|
T

A
|
A

A

A

A

Q

I

T

F

W

M

L

K

Q

S

E

-

N

-

Q

-

K

K

K

Y

Y

K

G

A

Y

N

T

Y

I

V

K

E

T

F

Y

P

S

N

D

I

G

D

V

N

H

A

M

Y

F

L

A

D

A

L

L

Q

S

A

S

G

G

N

N

L

I

D

A

A

G

V

A

V

M

H

D

D

E

S

V

P

P

N

V

V

I

L

S

Y

Y

Y

A

V

M

K

K

T

Q

A

-

G

-

G

-

G

G

K

Q

V

D

K

L

S

A

T

M

G

N

E

F

T

P

D

S

S

I

I

S

L

L

P

P

Q

G

K

G

Y

Y

G

G

F

F

A

A

L

V

Q

M

-

K

-

G

K

K

N

N

S

S

S

T

L

L

T

V

P

D

K

G

V

F

D

N

A

K

A

A

L

L

N

A

A

E

L

M

R

K

A

S

D

N

G

G

T

D

Y

Y

D

D

K

K

I

I

Y

L

F

K

K

K

W

Y

binding asparagine: D10 (= D34), Y13 (≠ F37), F51 (= F74), A68 (= A91), G69 (= G92), M70 (≠ I93), T71 (= T94), R76 (= R99), K115 (= K139), T118 (= T142), A119 (= A143)

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
32% identity, 88% coverage: 28:246/250 of query aligns to 5:232/238 of 1hslA

query
sites
1hslA

K

K

I

I

L

R

V

I

G

G

V

T

D

D

T

P

A

T

F
x
Y

V

A

P

P

F

F

E

E

F

S

K

K

Q

N

G

A

N

Q

-

G

K

E

Y

L

V

V

G

G

F

F

D

D

I

I

D

D

L

L

W

A

Q

K

A

E

I

L

A

C

K

K

K

R

M

I

N

N

V

T

S

Q

Y

C

E

T

L

F

R

V

P

E

M

N

D

P

F
x
L

S

D

G

A

L

L

I

I

P

P

G

S

L

L

Q

K

S

A

R

K

N

K

L

I

D

D

V

A

A

I

M

M

A
x
S

G
x
S

I
x
L

T
x
S

I

I

T

T

D

E

A

K

R
|
R

K

Q

Q

Q

V

E

V

I

D

A

F

F

S

T

D

D

G

K

Y

L

Y

Y

D

A

A

A

D

D

L

S

L

R

M

L

A

V

V

V

K

A

S

K

G

N

D

S

D

D

S

I

I

Q

T

P

K

T

F

V

S

A

E

S

L

L

A

K

G

G

K

K

K

R

V

V

G

G

L

V

K
x
L

Q

Q

G

G

T
|
T

A
x
T

A
x
Q

A

E

I

T

F

F

M

G

K

N

S

E

K

H

Y

W

K

A

A

P

N

K

Y

G

V

I

E

E

-

I

-

V

-

S

F

Y

P

Q

N

G

I

Q

D

D

N

N

A

I

Y

Y

L

S

D

D

L

L

Q

T

A

A

G

G

N

R

L

I

D

D

A

A

V

A

V

F

H

Q

D
|
D

S

E

P

V

N

A

V

A

L

S

Y

E

-

G

Y

F

V

L

K

K

T

Q

A

P

G

V

G

G

G

K

K

D

V

Y

K

K

S

F

T

G

G

G

-

P

-

A

-

V

E

K

T

D

D

E

S

K

I

L

L

F

P

G

Q

V

K

G

Y

T

G

G

F

M

A

G

L

L

Q

R

K

K

-

E

N

D

S

N

S

E

L

L

T

R

P

E

K

A

V

L

D

N

A

K

A

A

L

F

N

A

A

E

L

M

R

R

A

A

D

D

G

G

T

T

Y

Y

D

E

K

K

I

L

Y

A

F

K

K

K

W

Y

F

F

N

D

binding histidine: Y14 (≠ F37), L52 (≠ F74), S69 (≠ A91), S70 (≠ G92), L71 (≠ I93), S72 (≠ T94), R77 (= R99), L117 (≠ K139), T120 (= T142), T121 (≠ A143), Q122 (≠ A144), D161 (= D180)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
32% identity, 88% coverage: 28:246/250 of query aligns to 27:254/260 of P02910

query
sites
P02910

K

K

I

I

L