SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS22400 FitnessBrowser__Koxy:BWI76_RS22400 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

P46318 Lichenan-specific phosphotransferase enzyme IIB component; EIIB-Lic; PTS system lichenan-specific EIIB component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
42% identity, 89% coverage: 4:96/104 of query aligns to 3:95/102 of P46318

query
sites
P46318

I

I

M

L

L

L

C

V

C

C

S

A

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

K

S

K

K

M

M

I

E

A

K

E

S

A

A

E

Q

Q

E

R

Q

G

G

L

K

P

D

V

Y

E

T

I

I

N

W

A

A

F

V

G
x
S

V

G

A

D

E

S

F

V

D

Q

R

N

Q

H

V

I

G

D

H

K

Y

A

Q

D

V

V

V

L

L

L

G

G

P

P

Q

Q

V

V

K

R

Y

Y

M

M

Q

L

K

P

D

Q

L

L

Q

K

K

K

Q

L

A

G

D

E

K

S

Y

K

G

G

I

V

R

P

V

V

E

D

P

V

I

I

N

N

M

T

M

V

D

H

Y

Y

G

G

M

T

Q

C

K

N

G

G

G

A

A

E

V

V

L

L

D

K

F

S

A

A

S37 (≠ G38) modified: Phosphoserine

2wy2D Nmr structure of the iiachitobiose-iibchitobiose phosphoryl transition state complex of the n,n'-diacetylchitoboise brance of the e. Coli phosphotransferase system. (see paper)
45% identity, 88% coverage: 2:93/104 of query aligns to 2:91/103 of 2wy2D

query
sites
2wy2D

K

K

K

H

I

I

M

Y

L

L

C

F

C
|
C

S
|
S

A
|
A

G
|
G

M
|
M

S
|
S

T

T

S

S

L

L

V

V

K

S

K

K

M

M

I

R

A

A

E

Q

A

A

E

E

Q

K

R

Y

G

E

L

V

P

P

V

V

E

I

I

I

N

E

A

A

F

F

G

P

V

E

A

T

E

L

F

A

D

G

R

E

Q

K

V

G

G

Q

H

N

Y

A

Q

D

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

M

M

Q

L

K

P

D

E

L

I

Q

Q

K

R

Q

L

A

L

D

P

K

N

Y

K

G

P

I

V

R

E

V

V

E

-

P

-

I

I

N

D

M

S

M

L

D

L

Y

Y

G

G

M

K

Q

V

K

D

G

G

G

L

A

G

V

V

L

L

binding phosphite ion: C8 (= C8), S9 (= S9), A10 (= A10), G11 (= G11), M12 (= M12), S13 (= S13)

P69795 PTS system N,N'-diacetylchitobiose-specific EIIB component; EIIB-Chb; IVcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC 2.7.1.196 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 88% coverage: 2:93/104 of query aligns to 4:93/106 of P69795

query
sites
P69795

K

K

K

H

I

I

M

Y

L

L

C

F

C
|
C

S

S

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

K

S

K

K

M

M

I

R

A

A

E

Q

A

A

E

E

Q

K

R

Y

G

E

L

V

P

P

V

V

E

I

I

I

N

E

A

A

F

F

G

P

V

E

A

T

E

L

F

A

D

G

R

E

Q

K

V

G

G

Q

H

N

Y

A

Q

D

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

M

M

Q

L

K

P

D

E

L

I

Q

Q

K

R

Q

L

A

L

D

P

K

N

Y

K

G

P

I

V

R

E

V

V

E

-

P

-

I

I

N

D

M

S

M

L

D

L

Y

Y

G

G

M

K

Q

V

K

D

G

G

G

L

A

G

V

V

L

L

C10 (= C8) active site, Phosphocysteine intermediate; mutation to S: Unable to be phosphorylated by EIIA.

P11162 PTS system lactose-specific EIICB component; EIICB-Lac; EII-Lac; EC 2.7.1.207 from Staphylococcus aureus (see paper)
27% identity, 95% coverage: 4:102/104 of query aligns to 470:570/570 of P11162

query
sites
P11162

I

V

M

L

L

V

C

L

C
|
C

S

A

A

G

G

G

M

G

S

T

T

S

S

G

L

L

V

A

K

N

K

A

M

L

I

N

A

K

E

A

A

A

E

E

Q

E

R

Y

G

H

L

V

P

P

V

V

E

K

I

A

N

A

A

A

F

G

G

G

V

Y

A

G

E

A

F

H

D

M

R

D

Q

I

V

M

G

K

H

E

Y

Y

Q

Q

V

L

V

I

L

I

L

L

G

A

P

P

Q

Q

V

V

K

A

Y

S

M

N

Q

Y

K

E

D

D

L

I

Q

K

K

Q

Q

D

A

T

D

D

K

R

Y

L

G

G

I

I

R

K

V

L

E

A

P

K

I

T

N

Q

M

G

M

A

D

E

Y

Y

-

I

-

K

G

L

M

T

Q

R

K

D

G

G

G

Q

A

A

V

A

L

L

D

D

F

F

A

V

L

Q

S

Q

L

Q

I

F

D

E

N

N

C474 (= C8) modified: Phosphocysteine; by EIIA; mutation to S: Unable to be phosphorylated.

P24400 PTS system lactose-specific EIICB component; EIICB-Lac; EII-Lac; EC 2.7.1.207 from Lacticaseibacillus casei (Lactobacillus casei) (see paper)
25% identity, 91% coverage: 4:98/104 of query aligns to 479:575/577 of P24400

query
sites
P24400

I

V

M

L

L

V

C

L

C
|
C

S

A

A

G

G

G

M

G

S

T

T

S

S

G

L

I

L

L

V

A

K

N

K

A

M

L

I

N

A

K

E

L

A

S

E

K

Q

E

R

R

G

G

L

L

P

K

V

L

E

S

I

A

N

A

A

A

F

R

G

A

V

Y

A

G

E

Q

F

D

D

M

R

D

Q

L

V

I

G

K

H

D

Y

M

Q

N

V

M

V

V

L

I

L

L

G

A

P

P

Q

Q

V

M

K

E

Y

S

M

M

Q

K

K

G

D

N

L

L

Q

K

K

K

Q

I

A

T

D

D

K

K

Y

Y

G

G

I

V

R

K

V

L

E

V

P

T

I

T

N

T

M

G

M

R

D

Q

Y

Y

-

I

-

E

G

L

M

T

Q

N

K

N

G

G

G

D

A

M

V

A

L

L

D

D

F

F

A

V

L

E

S

S

C483 (= C8) modified: Phosphocysteine; by EIIA; mutation C->H,S: Unable to be phosphorylated.

Query Sequence

>BWI76_RS22400 FitnessBrowser__Koxy:BWI76_RS22400
MKKIMLCCSAGMSTSLLVKKMIAEAEQRGLPVEINAFGVAEFDRQVGHYQVVLLGPQVKY
MQKDLQKQADKYGIRVEPINMMDYGMQKGGAVLDFALSLIDNKN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory