SitesBLAST

9 C→A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
365 modified: Phosphoserine

1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
26% identity, 78% coverage: 27:153/162 of query aligns to 17:141/144 of 1j6tA

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binding phosphite ion: R46 (= R57), H62 (= H73), H108 (= H120)

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
27% identity, 88% coverage: 13:155/162 of query aligns to 3:141/143 of C0H3V2

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S74 (≠ V84) modified: Phosphoserine

P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
27% identity, 63% coverage: 52:153/162 of query aligns to 533:633/637 of P00550

query
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R538 (= R57) Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA
H554 (= H73) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

384 modified: Phosphocysteine; by EIIA

Query Sequence

>BWI76_RS25275 FitnessBrowser__Koxy:BWI76_RS25275
MINNDSALQLSNVLNQECTRSQVHCQSKKRALEIISELAAKQLGLSSQIVFEAILTREKM
GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH
LHTLSLVAKRLADKTICRRLRAAQSDEELYEIITEAGSNNEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory