SitesBLAST

Q17 (≠ L17) mutation to A: 45-fold decrease of the catalytic effiency.
R38 (= R38) binding
W48 (≠ L48) mutation to F: Little change in the affinity binding and catalytic efficiency, and it does not display major structural changes.; mutation to P: Little change in the affinity binding and catalytic efficiency. It exhibits substrate inhibition with oxalate. It does not display major structural changes.
R104 (≠ K106) binding
D169 (= D171) active site, Nucleophile; mutation to A: Loss of CoA-transferase activity.; mutation to E: Loss of CoA-transferase activity.; mutation to S: Loss of CoA-transferase activity.
G259 (vs. gap) mutation to A: 2.5-fold decrease of the catalytic effiency.
G260 (vs. gap) mutation to A: 25-fold decrease of the catalytic effiency. Reduction of the affinity binding for both formyl-CoA and oxalate.

1p5rA Formyl-coa transferase in complex with coenzyme a (see paper)
28% identity, 99% coverage: 3:383/383 of query aligns to 2:414/427 of 1p5rA

query
sites
1p5rA

R

K

P

P

L

L

E

D

G

G

L

I

L

N

V

V

L

L

E

D

F

F

C

T

Q
x
H

F
x
V

L
x
Q

A
|
A

G

G

P

P

S

A

A

C

G

T

L

Q

K

M

L

M

A

G

D

F

L

L

G

G

A

A

R

N

V

V

I

I

K

K

I

I

E
|
E

R
|
R

P

R

K

G

T

S

G

G

D

D

A

M

C

T

R

R

Q

G

L

W

S

L

I

Q

K

D

N

K

L

P

F

N

V

V

D

D

E

-

D

-

S

S

L

L

L

Y

F

F

H

T

T

M

I

F

N

N

R

C

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D
|
D

L
x
M

K
|
K

N

T

P

P

E

E

D

G

L

K

E

E

K

L

L

L

K

E

K

Q

L

M

I

I

S

K

K

K

A

A

D

D

V

V

M

M

T

V

H

E

N
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N

F
|
F

R

G

P

P

G

G

V
x
A

M

L

E

D

K
x
R

I
x
M

G

G

L

F

D

T

Y

W

Q

E

T

Y

V

I

Q

Q

N

E

I

L

N

N

P

P

K

R

I

V

V

I

Y

L

G

A

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S

V
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V

T

K

G

G

Y

Y

G

A

N

E

E

G

G

H

P

A

W

N

K

E

N

H

K

L

P
x
K

G
x
V

Q
x
Y

D
x
E

L

N

L

V

V

A

Q

Q

S

C

V

S

S

G

G

G

L

A

T

A

F

A

L

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T

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G

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F

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D

D

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G

P

P

P

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S

G

G

L

A

S

A

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S

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D
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D

I

S

M

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C

S

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G

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M

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H

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M

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I

G

G

I

I

M

L

A

A

L

L

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M

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R

A

H

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K

T

T

N

G

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E

A

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S

A

L
x
M

L

Q

E

D

S

A

I

V

L

L

D

N

V

-

Q

-

F

-

E

-

V

L

L

V

T

R

T

I

Y

K

L

L

N

R

D

D

G

Q

K

R

L

L

P

E

-

R

-

T

-

G

-

I

-

L

-

A

-

E

-

Y

-

P

-

Q

-

A

-

Q

-

P

-

N

-

F

-

A

-

F

D

D

R

R

S

D

G

G

A

N

K

P

G

L

S

S

A

F

H

D

A

N

Y

I

L

T

S

S

A

V

P

P

Y

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

G

G

M

W

Y

E

E

T

T

D

A

A

D

D

G

S

Y

Y

I

V

-

Y

-

F

A

T

M

I

A

A

M

A

G

N

N

M

L

W

P

P

N

Q

I

I

C

C

A

D

I

M

I

I

N

D

-

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C

P

D

E

I

W

T

K

D

D

L

-

Y

-

V

D

E

P

A

A

G

Y

S

N

A

T

F

F

E

E

N

G

R

R

-

V

D

D

Q

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L

L

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M

V

D

R

I

L

F

A

S

A

F

T

I

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T

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E

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N

A

T

D

K

K

T

D

W

K

V

F

E

E

L

V

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E

E

-

W

-

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N

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Y

G

G

I

I

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P

C

C

A

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V

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M

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E

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A

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Y

L

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Q

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K

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E

E

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V

L

D

D

E

G

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G

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K

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I

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T

T

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G

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I

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L

F

D

S

R

G

E

F

K

Q

L

P

F

E

A

I

S

T

K

R

A

-

A

A

P

P

K

L

L

L

G

G

G

E

N

H

T

T

S

D

D

E

I

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L

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K

E

E

F

L

E

G

L

L

N

D

active site: Q16 (≠ L17), E139 (≠ D142), D168 (= D171), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ Q15), V15 (≠ F16), Q16 (≠ L17), A17 (= A18), E36 (= E37), R37 (= R38), L71 (≠ A74), D72 (= D75), M73 (≠ L76), K74 (= K77), N95 (= N98), F96 (= F99), A100 (≠ V103), R103 (≠ K106), M104 (≠ I107), V123 (= V126), K136 (≠ P139), V137 (≠ G140), Y138 (≠ Q141), D168 (= D171), M199 (≠ L202)

2vjoA Formyl-coa transferase mutant variant q17a with aspartyl-coa thioester intermediates and oxalate (see paper)
28% identity, 99% coverage: 3:383/383 of query aligns to 2:414/427 of 2vjoA

query
sites
2vjoA

R

K

P

P

L

L

E

D

G

G

L

I

L

N

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V

L

L

E

D

F

F

C

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Q
x
H

F
x
V

L
x
A

A
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A

G

G

P

P

S

A

A

C

G

T

L

Q

K

M

L

M

A

G

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F

L

L

G

G

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A

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V

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I

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E
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E

R
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R

P

R

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G

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G

G

D

D

A
x
M

C

T

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R

Q

G

L

W

S

L

I

Q

K

D

N

K

L

P

F

N

V

V

D

D

E

-

D

-

S

S

L

L

L

Y

F

F

H

T

T

M

I

F

N

N

R

C

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D
|
D

L
x
M

K
|
K

N

T

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P

E

E

D

G

L

K

E

E

K

L

L

L

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Q

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M

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I

S

K

K

K

A

A

D

D

V

V

M

M

T

V

H

E

N
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F
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F

R
x
G

P

P

G

G

V
x
A

M

L

E

D

K
x
R

I
x
M

G

G

L

F

D

T

Y

W

Q

E

T

Y

V

I

Q

Q

N

E

I

L

N

N

P

P

K

R

I

V

V

I

Y

L

G

A

V

S

V
|
V

T
x
K

G

G

Y

Y

G

A

N

E

E

G

G

H

P

A

W

N

K

E

N

H

K

L

P
x
K

G
x
V

Q
x
Y

D
x
E

L

N

L

V

V

A

Q

Q

S

C

V

S

S

G

G

G

L

A

T

A

F

A

L

T

S

T

G

G

V

F

D

W

V

D

D

G

G

P

P

P

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T

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V

F

S

G

G

L

A

S

A

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G

D
|
D

I

S

M

N

C

S

G

G

N

M

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H

L

L

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M

Q

I

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G

I

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M

L

A

A

L

L

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R

A

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A

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A

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x
M

L

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D

S

A

I

V

L

L

D

N

V

-

Q

-

F

-

E

-

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T

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A

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A

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Q

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N

-

F

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A

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F

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D

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R

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D

G

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x
G

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G

G

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E

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A

A

D

D

G

S

Y

Y

I

V

-

Y

-

F

A

T

M

I

A

A

M

A

G

N

N

M

L

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P

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N

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I

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C

A

D

I

M

I

I

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D

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K

C

P

D

E

I

W

T

K

D

D

L

-

Y

-

V

D

E

P

A

A

G

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A

T

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F

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E

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G

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-

V

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M

V

D

R

I

L

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S

A

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T

D

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K

T

D

W

K

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F

E

E

L

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T

E

E

-

W

-

A

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Y

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G

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E

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A

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H

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T

S

Y

L

K

Q

K

K

L

V

D

G

I

T

A

V

Q

V

E

E

L

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N

V

L

D

D

E

G

I

G

R

K

G

T

N

I

H

K

L

T

T

T

V

V

G

S

A

P

P

I

F

R

K

L

F

D

S

R

G

E

F

K

Q

L

P

F

E

A

I

S

T

K

R

A

-

A

A

P

P

K

L

L

L

G

G

G

E

N

H

T

T

S

D

D

E

I

V

N

L

K

K

E

E

F

L

E

G

L

L

N

D

active site: A16 (≠ L17), E139 (≠ D142), D168 (= D171), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ Q15), V15 (≠ F16), A16 (≠ L17), A17 (= A18), E36 (= E37), R37 (= R38), M43 (≠ A44), L71 (≠ A74), D72 (= D75), M73 (≠ L76), K74 (= K77), N95 (= N98), F96 (= F99), G97 (≠ R100), A100 (≠ V103), R103 (≠ K106), M104 (≠ I107), V123 (= V126), K124 (≠ T127), K136 (≠ P139), V137 (≠ G140), Y138 (≠ Q141), D168 (= D171), M199 (≠ L202)
binding oxalate ion: G257 (vs. gap), G258 (vs. gap), G259 (vs. gap), G260 (vs. gap), Q261 (vs. gap)

2vjkA Formyl-coa transferase with aspartyl-coa thioester intermediate derived from oxalyl-coa (see paper)
28% identity, 99% coverage: 3:383/383 of query aligns to 2:414/427 of 2vjkA

query
sites
2vjkA

R

K

P

P

L

L

E

D

G

G

L

I

L

N

V

V

L

L

E

D

F

F

C

T

Q
x
H

F
x
V

L
x
Q

A
|
A

G

G

P

P

S

A

A

C

G

T

L

Q

K

M

L

M

A

G

D

F

L

L

G

G

A

A

R

N

V

V

I

I

K

K

I

I

E
|
E

R
|
R

P

R

K

G

T

S

G

G

D

D

A

M

C

T

R

R

Q

G

L

W

S

L

I

Q

K

D

N

K

L

P

F

N

V

V

D

D

E

-

D

-

S

S

L

L

L

Y

F

F

H

T

T

M

I

F

N

N

R

C

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D
|
D

L
x
M

K
|
K

N

T

P

P

E

E

D

G

L

K

E

E

K

L

L

L

K

E

K

Q

L

M

I

I

S

K

K

K

A

A

D

D

V

V

M

M

T

V

H

E

N
|
N

F
|
F

R
x
G

P

P

G

G

V
x
A

M

L

E

D

K
x
R

I
x
M

G

G

L

F

D

T

Y

W

Q

E

T

Y

V

I

Q

Q

N

E

I

L

N

N

P

P

K

R

I

V

V

I

Y

L

G

A

V

S

V
|
V

T

K

G

G

Y

Y

G

A

N

E

E

G

G

H

P

A

W

N

K

E

N

H

K

L

P
x
K

G
x
V

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x
Y

D
x
E

L

N

L

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V

A

Q

Q

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V

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S

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G

G

L

A

T

A

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A

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T

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G

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G

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A

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D

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L

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A

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L

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R

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G

K

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K

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E

A

V

V

S

A

L
x
M

L

Q

E

D

S

A

I

V

L

L

D

N

V

-

Q

-

F

-

E

-

V

L

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T

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T

I

Y

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R

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E

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I

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A

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N

-

F

-

A

-

F

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D

R

R

S

D

G

G

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P

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A

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D

A

N

Y

I

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x
G

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G

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W

-

M

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L

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C

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K

G

G

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W

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E

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A

A

D

D

G

S

Y

Y

I

V

-

Y

-

F

A

T

M

I

A

A

M

A

G

N

N

M

L

W

P

P

N

Q

I

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C

C

A
x
D

I

M

I

I

N
x
D

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K

C

P

D

E

I

W

T

K

D

D

L

-

Y

-

V

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E

P

A

A

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A

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F

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R

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L

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A

S

A

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K

E

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A

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D

K

K

T

D

W

K

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F

E

E

L

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E

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W

-

A

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Y

G

G

I

I

W

P

C

C

A

G

E

P

V

V

L

M

N

S

Y

M

Q

K

K

E

A

L

T

A

A

H

L

D

S

P

T

S

Y

L

K

Q

K

K

L

V

D

G

I

T

A

V

Q

V

E

E

L

V

N

V

L

D

D

E

G

I

G

R

K

G

T

N

I

H

K

L

T

T

T

V

V

G

S

A

P

P

I

F

R

K

L

F

D

S

R

G

E

F

K

Q

L

P

F

E

A

I

S

T

K

R

A

-

A

A

P

P

K

L

L

L

G

G

G

E

N

H

T

T

S

D

D

E

I

V

N

L

K

K

E

E

F

L

E

G

L

L

N

D

active site: Q16 (≠ L17), E139 (≠ D142), D168 (= D171), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ Q15), V15 (≠ F16), Q16 (≠ L17), A17 (= A18), E36 (= E37), R37 (= R38), L71 (≠ A74), D72 (= D75), M73 (≠ L76), K74 (= K77), N95 (= N98), F96 (= F99), G97 (≠ R100), A100 (≠ V103), R103 (≠ K106), M104 (≠ I107), V123 (= V126), K136 (≠ P139), V137 (≠ G140), Y138 (≠ Q141), D168 (= D171), M199 (≠ L202), G259 (vs. gap), G260 (vs. gap)
binding magnesium ion: D293 (≠ A264), D296 (≠ N267)

1t3zA Formyl-coa tranferase mutant asp169 to ser (see paper)
28% identity, 99% coverage: 3:383/383 of query aligns to 2:414/427 of 1t3zA

query
sites
1t3zA

R

K

P

P

L

L

E

D

G

G

L

I

L

N

V

V

L

L

E

D

F

F

C

T

Q
x
H

F
x
V

L
x
Q

A
|
A

G

G

P

P

S

A

A

C

G

T

L

Q

K

M

L

M

A

G

D

F

L

L

G

G

A

A

R

N

V

V

I

I

K

K

I

I

E
|
E

R
|
R

P

R

K

G

T

S

G

G

D

D

A

M

C

T

R

R

Q

G

L

W

S

L

I

Q

K

D

N

K

L

P

F

N

V

V

D

D

E

-

D

-

S

S

L

L

L

Y

F

F

H

T

T

M

I

F

N

N

R

C

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D

D

L
x
M

K
|
K

N

T

P

P

E

E

D

G

L

K

E

E

K

L

L

L

K

E

K

Q

L

M

I

I

S

K

K

K

A

A

D

D

V

V

M

M

T

V

H

E

N
|
N

F
|
F

R
x
G

P

P

G

G

V
x
A

M

L

E

D

K
x
R

I
x
M

G

G

L

F

D

T

Y

W

Q

E

T

Y

V

I

Q

Q

N

E

I

L

N

N

P

P

K

R

I

V

V

I

Y

L

G

A

V

S

V
|
V

T

K

G

G

Y

Y

G

A

N

E

E

G

G

H

P

A

W

N

K

E

N

H

K

L

P
x
K

G
x
V

Q
x
Y

D
x
E

L

N

L

V

V

A

Q

Q

S

C

V

S

S

G

G

G

L

A

T

A

F

A

L

T

S

T

G

G

V

F

D

W

V

D

D

G

G

P

P

P

V

T

P

V

F

S

G

G

L

A

S

A

V

L

S

G

D
x
S

I

S

M

N

C

S

G

G

N

M

H

H

L

L

V

M

Q

I

G

G

I

I

M

L

A

A

L

L

I

E

K

I

R

R

A

H

K

K

T

T

N

G

K

R

S

G

V

Q

L

K

V

V

E

A

V

V

S

A

L
x
M

L

Q

E

D

S

A

I

V

L

L

D

N

V

-

Q

-

F

-

E

-

V

L

L

V

T

R

T

I

Y

K

L

L

N

R

D

D

G

Q

K

R

L

L

P

E

-

R

-

T

-

G

-

I

-

L

-

A

-

E

-

Y

-

P

-

Q

-

A

-

Q

-

P

-

N

-

F

-

A

-

F

D

D

R

R

S

D

G

G

A

N

K

P

G

L

S

S

A

F

H

D

A

N

Y

I

L

T

S

S

A

V

P

P

Y

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

G

G

M

W

Y

E

E

T

T

D

A

A

D

D

G

S

Y

Y

I

V

-

Y

-

F

A

T

M

I

A

A

M

A

G

N

N

M

L

W

P

P

N

Q

I

I

C

C

A

D

I

M

I

I

N

D

-

K

C

P

D

E

I

W

T

K

D

D

L

-

Y

-

V

D

E

P

A

A

G

Y

S

N

A

T

F

F

E

E

N

G

R

R

-

V

D

D

Q

K

L

L

I

M

V

D

R

I

L

F

A

S

A

F

T

I

F

E

K

T

K

K

E

F

N

A

T

D

K

K

T

D

W

K

V

F

E

E

L

V

L

T

E

E

-

W

-

A

N

Q

H

Y

G

G

I

I

W

P

C

C

A

G

E

P

V

V

L

M

N

S

Y

M

Q

K

K

E

A

L

T

A

A

H

L

D

S

P

T

S

Y

L

K

Q

K

K

L

V

D

G

I

T

A

V

Q

V

E

E

L

V

N

V

L

D

D

E

G

I

G

R

K

G

T

N

I

H

K

L

T

T

T

V

V

G

S

A

P

P

I

F

R

K

L

F

D

S

R

G

E

F

K

Q

L

P

F

E

A

I

S

T

K

R

A

-

A

A

P

P

K

L

L

L

G

G

G

E

N

H

T

T

S

D

D

E

I

V

N

L

K

K

E

E

F

L

E

G

L

L

N

D

active site: Q16 (≠ L17), E139 (≠ D142), S168 (≠ D171), G259 (vs. gap), G260 (vs. gap)
binding oxidized coenzyme a: H14 (≠ Q15), V15 (≠ F16), Q16 (≠ L17), A17 (= A18), E36 (= E37), R37 (= R38), L71 (≠ A74), M73 (≠ L76), K74 (= K77), N95 (= N98), F96 (= F99), G97 (≠ R100), A100 (≠ V103), R103 (≠ K106), M104 (≠ I107), V123 (= V126), K136 (≠ P139), V137 (≠ G140), Y138 (≠ Q141), E139 (≠ D142), S168 (≠ D171), M199 (≠ L202)

1pt8A Crystal structure of the yfdw gene product of e. Coli, in complex with oxalate and acetyl-coa (see paper)
34% identity, 55% coverage: 1:209/383 of query aligns to 1:207/416 of 1pt8A

query
sites
1pt8A

M

M

N

S

R

T

P

P

L

L

E

Q

G

G

L

I

L

K

V

V

L

L

E

D

F

F

C

T

Q

G

F
x
V

L
x
Q

A
x
S

G

G

P

P

S

S

A

C

G

T

L

Q

K

M

L

L

A

A

D

W

L

F

G

G

A

A

R

D

V

V

I

I

K

K

I

I

E
|
E

R
|
R

P

P

K

G

T

V

G

G

D

D

A

V

C

T

R

R

Q

H

L

-

S
x
Q

I
x
L

K

R

N

D

L

I

F

-

V

P

D

D

E

I

D

D

S

A

L

L

L

Y

F

F

H

T

T

M

I

L

N

N

R

S

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D
x
N

L
x
T

K
|
K

N

T

P

A

E

E

D

G

L

K

E

E

K

V

L

M

K

E

K

K

L

L

I

I

S

R

K

E

A

A

D

D

V

I

M

L

T

V

H

E

N
|
N

F
|
F

R
x
H

P

P

G

G

V
x
A

M

I

E

D

K

H

I

M

G

G

L

F

D

T

Y

W

Q

E

T

H

V

I

Q

Q

N

E

I

I

N

N

P

P

K

R

I

L

V

I

Y

F

G

G

V

S

V
x
I

T

K

G

G

Y

F

G

D

N

E

E

C

G

S

P

P

W

Y

K

V

N

N

K

V

P
x
K

G
x
A

Q
x
Y

D
x
E

L

N

L

V

V

A

Q

Q

S

A

V

A

S

G

G

G

L

A

T

A

F

S

L

T

S

T

G

G

V

F

D

W

V

D

D

G

G

P

P

P

V

L

P

V

F

S

G

A

L

A

S

A

V

L

S

G

D
|
D

I

S

M

N

C

T

G

G

N

M

H

H

L

L

V

L

Q

I

G

G

I

L

M

L

A

A

L

L

I

L

K

H

R

R

A

E

K

K

T

T

N

G

K

R

S

G

V

Q

L

R

V

V

E

T

V

M

S

S

L
x
M

L

Q

E

D

S

A

I

V

L

L

D

N

V

L

active site: Q17 (≠ L17), E140 (≠ D142), D169 (= D171)
binding acetyl coenzyme *a: V16 (≠ F16), Q17 (≠ L17), S18 (≠ A18), E37 (= E37), R38 (= R38), L72 (≠ A74), N73 (≠ D75), T74 (≠ L76), K75 (= K77), N96 (= N98), F97 (= F99), H98 (≠ R100), A101 (≠ V103), I124 (≠ V126), K137 (≠ P139), A138 (≠ G140), Y139 (≠ Q141), D169 (= D171), M200 (≠ L202)
binding oxalate ion: Q48 (≠ S49), L49 (≠ I50)

Sites not aligning to the query:

active site: 248, 249
binding acetyl coenzyme *a: 248, 249, 273
binding oxalate ion: 226, 249, 250, 251

P69902 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; Formyl-CoA transferase; EC 2.8.3.16 from Escherichia coli (strain K12) (see paper)
34% identity, 55% coverage: 1:209/383 of query aligns to 1:207/416 of P69902

query
sites
P69902

M

M

N

S

R

T

P

P

L

L

E

Q

G

G

L

I

L

K

V

V

L

L

E

D

F

F

C

T

Q

G

F

V

L

Q

A

S

G

G

P

P

S

S

A

C

G

T

L

Q

K

M

L

L

A

A

D

W

L

F

G

G

A

A

R

D

V

V

I

I

K

K

I

I

E

E

R
|
R

P

P

K

G

T

V

G

G

D

D

A

V

C

T

R

R

Q

H

L

-

S

Q

I

L

K

R

N

D

L

I

F

-

V

P

D

D

E

I

D

D

S

A

L

L

L

Y

F

F

H

T

T

M

I

L

N

N

R

S

N

N

K

K

E

R

S

S

Y

I

A

E

A

L

D

N

L

T

K

K

N

T

P

A

E

E

D

G

L

K

E

E

K

V

L

M

K

E

K

K

L

L

I

I

S

R

K

E

A

A

D

D

V

I

M

L

T

V

H

E

N

N

F

F

R

H

P

P

G

G

V

A

M

I

E

D

K

H

I

M

G

G

L

F

D

T

Y

W

Q

E

T

H

V

I

Q

Q

N

E

I

I

N

N

P

P

K

R

I

L

V

I

Y

F

G

G

V

S

V

I

T

K

G

G

Y

F

G

D

N

E

E

C

G

S

P

P

W

Y

K

V

N

N

K

V

P

K

G

A

Q

Y

D

E

L

N

L

V

V

A

Q

Q

S

A

V

A

S

G

G

G

L

A

T

A

F

S

L

T

S

T

G

G

V

F

D

W

V

D

D

G

G

P

P

P

V

L

P

V

F

S

G

A

L

A

S

A

V

L

S

G

D

D

I

S

M

N

C

T

G

G

N

M

H

H

L

L

V

L

Q

I

G

G

I

L

M

L

A

A

L

L

I

L

K

H

R

R

A

E

K

K

T

T

N

G

K

R

S

G

V

Q

L

R

V

V

E

T

V

M

S

S

L

M

L

Q

E

D

S

A

I

V

L

L

D

N

V

L

R38 (= R38) binding

1q6yA Hypothetical protein yfdw from e. Coli bound to coenzyme a (see paper)
33% identity, 55% coverage: 1:209/383 of query aligns to 1:207/417 of 1q6yA

query
sites
1q6yA

M

L

N

S

R

T

P

P

L

L

E

Q

G

G

L

I

L

K

V

V

L

L

E

D

F
|
F

C

T

Q

G

F
x
V

L
x
Q

A
x
S

G

G

P

P

S

S

A

C

G

T

L

Q

K

M

L

L

A

A

D

W

L

F

G

G

A

A

R

D

V

V

I

I

K

K

I

I

E
|
E

R
|
R

P

P

K

G

T

V

G

G

D

D

A

V

C

T

R

R

Q

H

L

-

S

Q

I

L

K

R

N

D

L

I

F

-

V

P

D

D

E

I

D

D

S

A

L

L

L

Y

F

F

H

T

T

M

I

L

N

N

R

S

N

N

K

K

E

R

S

S

Y

I

A

E

A
x
L

D
x
N

L
x
T

K
|
K

N

T

P

A

E

E

D

G

L

K

E

E

K

V

L

M

K

E

K

K

L

L

I

I

S

R

K

E

A

A

D

D

V

I

M

L

T

V

H

E

N
|
N

F
|
F

R
x
H

P

P

G

G

V
x
A

M

I

E

D

K

H

I
x
M

G

G

L

F

D

T

Y

W

Q

E

T

H

V

I

Q

Q

N

E

I

I

N

N

P

P

K

R

I

L

V

I

Y

F

G

G

V

S

V
x
I

T
x
K

G
|
G

Y

F

G

D

N

E

E

C

G

S

P

P

W

Y

K

V

N

N

K

V

P
x
K

G
x
A

Q
x
Y

D
x
E

L

N

L

V

V

A

Q

Q

S

A

V

A

S

G

G

G

L

A

T

A

F

S

L

T

S

T

G

G

V

F

D

W

V

D

D

G

G

P

P

P

V

L

P

V

F

S

G

A

L

A

S

A

V

L

S

G

D
|
D

I

S

M

N

C

T

G

G

N

M

H

H

L

L

V

L

Q

I

G

G

I

L

M

L

A

A

L

L

I

L

K

H

R

R

A

E

K

K

T

T

N

G

K

R

S

G

V

Q

L

R

V

V

E

T

V

M

S

S

L
x
M

L

Q

E

D

S

A

I

V

L

L

D

N

V

L

active site: Q17 (≠ L17), E140 (≠ D142), D169 (= D171)
binding coenzyme a: F13 (= F13), V16 (≠ F16), Q17 (≠ L17), S18 (≠ A18), E37 (= E37), R38 (= R38), L72 (≠ A74), N73 (≠ D75), T74 (≠ L76), K75 (= K77), N96 (= N98), F97 (= F99), H98 (≠ R100), A101 (≠ V103), M105 (≠ I107), I124 (≠ V126), K125 (≠ T127), G126 (= G128), K137 (≠ P139), A138 (≠ G140), Y139 (≠ Q141), D169 (= D171), M200 (≠ L202)

Sites not aligning to the query:

active site: 248, 249

O06543 Alpha-methylacyl-CoA racemase; AMACR; MtMCR; EC 5.1.99.4 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
33% identity, 66% coverage: 1:254/383 of query aligns to 1:237/360 of O06543

query
sites
O06543

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R38 (= R38) binding
R52 (= R67) mutation to A: 15.7% of wild-type activity.
I56 (≠ S71) mutation to P: 28.8% of wild-type activity.
ADLK 59:62 (= ADLK 74:77) binding
E82 (≠ H97) mutation to A: 12.5% of wild-type activity.
GYR 83:85 (≠ NFR 98:100) binding
R91 (≠ K106) binding ; mutation to A: 19.9% of wild-type activity.
M111 (≠ V126) mutation to P: 5.2% of wild-type activity.
GHDINY 125:130 (≠ GQDLLV 140:145) binding
H126 (≠ Q141) mutation to A: 4.5% of wild-type activity.
D156 (= D171) mutation to A: 17.6 of wild-type activity.
D190 (≠ E204) mutation to A: 3.3% of wild-type activity.

Sites not aligning to the query:

241 E→A: 2.1% of wild-type activity.
297 C→A: 6.2% of wild-type activity.
312 H→A: 10.1% of wild-type activity.

1pt5A Crystal structure of gene yfdw of e. Coli (see paper)
34% identity, 54% coverage: 4:209/383 of query aligns to 3:206/415 of 1pt5A

query
sites
1pt5A

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active site: Q16 (≠ L17), E139 (≠ D142), D168 (= D171)
binding acetyl coenzyme *a: F12 (= F13), V15 (≠ F16), S17 (≠ A18), E36 (= E37), R37 (= R38), L71 (≠ A74), N72 (≠ D75), T73 (≠ L76), K74 (= K77), N95 (= N98), F96 (= F99), H97 (≠ R100), A100 (≠ V103), I123 (≠ V126), K124 (≠ T127), K136 (≠ P139), A137 (≠ G140), Y138 (≠ Q141), E139 (≠ D142), D168 (= D171), M199 (≠ L202)

Sites not aligning to the query:

active site: 247, 248

2yimA The enolisation chemistry of a thioester-dependent racemase: the 1.4 a crystal structure of a complex with a planar reaction intermediate analogue (see paper)
32% identity, 66% coverage: 4:254/383 of query aligns to 3:232/355 of 2yimA

query
sites
2yimA

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active site: G16 (≠ L17), D122 (= D142), D151 (= D171), G214 (= G232), G215 (≠ S233)
binding 2-methylacetoacetyl coa: I15 (≠ F16), G16 (≠ L17), P17 (≠ A18), D36 (≠ E37), R37 (= R38), A54 (= A74), D55 (= D75), L56 (= L76), K57 (= K77), G78 (≠ N98), Y79 (≠ F99), R80 (= R100), V83 (= V103), R86 (≠ K106), L87 (≠ I107), T107 (= T127), A119 (≠ P139), G120 (= G140), H121 (≠ Q141), D122 (= D142), Y125 (≠ V145), N147 (≠ L167), D151 (= D171), M183 (≠ L202), L212 (vs. gap), Y219 (= Y237)

Sites not aligning to the query:

binding 2-methylacetoacetyl coa: 239

2gd6A The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
32% identity, 66% coverage: 4:254/383 of query aligns to 3:231/354 of 2gd6A

query
sites
2gd6A

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