SitesBLAST
Comparing CA265_RS15335 FitnessBrowser__Pedo557:CA265_RS15335 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
68% identity, 98% coverage: 4:310/314 of query aligns to 4:306/306 of 5z75A
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), C11 (≠ N11), G12 (= G12), Q13 (= Q13), I14 (= I14), D35 (= D35), I36 (= I36), L49 (≠ V52), V51 (= V54), L72 (= L75), A73 (= A76), L76 (= L79), P113 (= P116), S115 (= S118), Y140 (= Y143), K144 (= K147), Y167 (= Y170), P168 (= P171), L170 (= L173), P176 (≠ A179), P177 (= P180)
7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
63% identity, 99% coverage: 3:312/314 of query aligns to 1:307/310 of 7cgvA
- binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), Q11 (= Q13), I12 (= I14), D33 (= D35), I34 (= I36), R35 (= R37), L48 (≠ V52), D49 (≠ N53), V50 (= V54), A72 (= A76), A73 (= A77), L75 (= L79), P112 (= P116), S114 (= S118), Y139 (= Y143), K143 (= K147), Y166 (= Y170), I169 (≠ L173)
7eprB Partial consensus l-threonine 3-dehydrogenase (c-change) (see paper)
60% identity, 99% coverage: 2:313/314 of query aligns to 1:309/310 of 7eprB
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), Q12 (= Q13), I13 (= I14), D34 (= D35), I35 (= I36), R36 (= R37), D50 (≠ N53), A51 (≠ V54), L72 (= L75), A73 (= A76), A74 (= A77), L90 (= L93), P113 (= P116), Y140 (= Y143), K144 (= K147), Y167 (= Y170), L170 (= L173)
2yy7A Crystal structure of thermolabile l-threonine dehydrogenase from flavobacterium frigidimaris kuc-1 (see paper)
59% identity, 99% coverage: 1:312/314 of query aligns to 1:311/312 of 2yy7A
- binding glycerol: S118 (= S118), Y143 (= Y143), G183 (= G183), T184 (= T184), T185 (= T185), W279 (= W280)
- binding nicotinamide-adenine-dinucleotide: C11 (≠ N11), G12 (= G12), Q13 (= Q13), I14 (= I14), D35 (= D35), I36 (= I36), R37 (= R37), V52 (= V52), N53 (= N53), A54 (≠ V54), M75 (≠ L75), A76 (= A76), L93 (= L93), P116 (= P116), S118 (= S118), Y143 (= Y143), K147 (= K147), Y170 (= Y170), L173 (= L173)
7epsA Partial consensus l-threonine 3-dehydrogenase (e-change) (see paper)
55% identity, 99% coverage: 4:313/314 of query aligns to 2:308/310 of 7epsA
- binding nicotinamide-adenine-dinucleotide: N9 (= N11), G10 (= G12), Q11 (= Q13), I12 (= I14), D33 (= D35), V34 (≠ I36), L48 (≠ V52), A50 (≠ V54), L71 (= L75), A72 (= A76), A73 (= A77), L89 (= L93), P112 (= P116), Y139 (= Y143), K143 (= K147), Y166 (= Y170), L169 (= L173)
- binding threonine: S76 (= S80), S114 (= S118), Y139 (= Y143), G179 (= G183), T180 (= T184), T181 (= T185)
5y1gA Monomeric l-threonine 3-dehydrogenase from metagenome database (akb and nadh bound form) (see paper)
50% identity, 99% coverage: 5:314/314 of query aligns to 4:307/310 of 5y1gA
- binding 2-amino-3-ketobutyric acid: S76 (= S80), G178 (= G183), T179 (= T184), T180 (= T185), W273 (= W280)
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), Q12 (= Q13), I13 (= I14), D34 (= D35), L35 (≠ I36), D49 (≠ N53), I50 (≠ V54), L71 (= L75), A72 (= A76), A73 (= A77), L75 (= L79), Y138 (= Y143), K142 (= K147), Y165 (= Y170), L168 (= L173)
5y1fA Monomeric l-threonine 3-dehydrogenase from metagenome database (NAD+ bound form) (see paper)
50% identity, 99% coverage: 5:314/314 of query aligns to 5:308/311 of 5y1fA
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), Q13 (= Q13), I14 (= I14), D35 (= D35), L36 (≠ I36), D50 (≠ N53), I51 (≠ V54), L72 (= L75), A73 (= A76), A74 (= A77), L76 (= L79), S114 (= S118), Y139 (= Y143), K143 (= K147), Y166 (= Y170), L169 (= L173)
5y1eA Monomeric l-threonine 3-dehydrogenase from metagenome database (l-ser and NAD+ bound form) (see paper)
50% identity, 99% coverage: 5:314/314 of query aligns to 2:305/308 of 5y1eA
- binding nicotinamide-adenine-dinucleotide: G6 (= G9), G9 (= G12), Q10 (= Q13), I11 (= I14), D32 (= D35), L33 (≠ I36), D47 (≠ N53), I48 (≠ V54), L69 (= L75), A70 (= A76), A71 (= A77), L73 (= L79), P109 (= P116), Y136 (= Y143), K140 (= K147), Y163 (= Y170), L166 (= L173)
- binding serine: S74 (= S80), S111 (= S118), Y136 (= Y143), G176 (= G183), T177 (= T184), T178 (= T185)
3wmwB Gale-like l-threonine dehydrogenase from cupriavidus necator (apo form) (see paper)
51% identity, 99% coverage: 4:313/314 of query aligns to 3:278/278 of 3wmwB
Q8K3F7 L-threonine 3-dehydrogenase, mitochondrial; EC 1.1.1.103 from Mus musculus (Mouse) (see paper)
49% identity, 92% coverage: 21:309/314 of query aligns to 74:360/373 of Q8K3F7
- DIR 88:90 (= DIR 35:37) binding
- NI 106:107 (≠ NV 53:54) binding
- S133 (= S80) mutation to A: Decreased L-threonine 3-dehydrogenase activity.
- R180 (≠ K128) mutation to K: Decreased L-threonine 3-dehydrogenase activity. No effect on protein NAD(+)-binding. No gross effect on protein folding. No effect on protein stability.
- Y195 (= Y143) binding
- K199 (= K147) binding
- I225 (≠ L173) binding
- T237 (= T185) mutation to A: Decreased L-threonine 3-dehydrogenase activity.
- M333 (≠ R282) mutation to A: Decreased L-threonine 3-dehydrogenase activity.; mutation to E: Decreased L-threonine 3-dehydrogenase activity. Decreased affinity for L-threonine.
Sites not aligning to the query:
4yr9A Mouse tdh with NAD+ bound (see paper)
49% identity, 92% coverage: 21:309/314 of query aligns to 20:305/308 of 4yr9A
- binding glycerol: Y141 (= Y143), T182 (= T185), W276 (= W280)
- binding nicotinamide-adenine-dinucleotide: D34 (= D35), I35 (= I36), R36 (= R37), A51 (≠ V52), N52 (= N53), I53 (≠ V54), Y74 (≠ L75), S75 (≠ A76), A76 (= A77), L78 (= L79), P114 (= P116), T116 (≠ S118), Y141 (= Y143), K145 (= K147), Y168 (= Y170), I171 (≠ L173)
Sites not aligning to the query:
6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
48% identity, 98% coverage: 4:312/314 of query aligns to 2:303/307 of 6jygD
- binding citrate anion: Q11 (= Q13), L73 (= L79), S74 (= S80), Y136 (= Y143), G176 (= G183), T178 (= T185), D179 (= D186)
- binding nicotinamide-adenine-dinucleotide: G7 (= G9), T9 (≠ N11), G10 (= G12), Q11 (= Q13), I12 (= I14), D33 (= D35), I34 (= I36), K35 (≠ R37), D47 (≠ N53), V48 (= V54), M69 (≠ L75), A70 (= A76), S71 (≠ A77), L73 (= L79), V87 (≠ L93), P109 (= P116), S110 (= S117), T111 (≠ S118), Y136 (= Y143), K140 (= K147), Y163 (= Y170), V166 (≠ L173)
3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
42% identity, 99% coverage: 5:314/314 of query aligns to 2:307/315 of 3a1nA
- active site: T112 (≠ S118), Y137 (= Y143), K141 (= K147)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ N11), G9 (= G12), Q10 (= Q13), I11 (= I14), D32 (= D35), I33 (= I36), L46 (≠ V52), D47 (≠ N53), L69 (= L75), A70 (= A76), G71 (≠ A77), L73 (= L79), S111 (= S117), Y137 (= Y143), K141 (= K147), Y164 (= Y170)
3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
42% identity, 99% coverage: 5:314/314 of query aligns to 2:307/311 of 3a4vA
- active site: T112 (≠ S118), Y137 (= Y143), K141 (= K147)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ N11), G9 (= G12), Q10 (= Q13), I11 (= I14), D32 (= D35), I33 (= I36), L46 (≠ V52), D47 (≠ N53), V48 (= V54), L69 (= L75), A70 (= A76), G71 (≠ A77), L73 (= L79), P110 (= P116), S111 (= S117), T112 (≠ S118), Y137 (= Y143), K141 (= K147), Y164 (= Y170), I167 (≠ L173)
- binding pyruvic acid: S74 (= S80), Y137 (= Y143), T178 (= T184), T179 (= T185), W273 (= W280)
3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
42% identity, 99% coverage: 5:314/314 of query aligns to 2:307/314 of 3ajrA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ N11), G9 (= G12), Q10 (= Q13), I11 (= I14), D32 (= D35), I33 (= I36), L46 (≠ V52), D47 (≠ N53), V48 (= V54), L69 (= L75), A70 (= A76), G71 (≠ A77), L73 (= L79), P110 (= P116), S111 (= S117), T112 (≠ S118), F137 (≠ Y143), K141 (= K147), Y164 (= Y170), I167 (≠ L173)
- binding (3R)-3-hydroxy-L-norvaline: S74 (= S80), T112 (≠ S118), I113 (= I119), F137 (≠ Y143), G177 (= G183), T178 (= T184), T179 (= T185)
3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
42% identity, 99% coverage: 5:314/314 of query aligns to 2:307/315 of 3a9wA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ N11), G9 (= G12), Q10 (= Q13), I11 (= I14), D32 (= D35), I33 (= I36), L46 (≠ V52), D47 (≠ N53), V48 (= V54), L69 (= L75), A70 (= A76), L73 (= L79), V87 (≠ L93), P110 (= P116), S111 (= S117), K141 (= K147), Y164 (= Y170), I167 (≠ L173)
- binding threonine: S74 (= S80), T112 (≠ S118), F137 (≠ Y143), G177 (= G183), T178 (= T184), T179 (= T185)
5k50C Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to NAD+ and l-allo-threonine refined to 2.23 angstroms (see paper)
37% identity, 98% coverage: 4:312/314 of query aligns to 2:310/319 of 5k50C
- binding allo-threonine: M79 (≠ L79), S80 (= S80), T117 (≠ S118), Y142 (= Y143), A183 (≠ T184), W278 (= W280)
- binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), Q11 (= Q13), I12 (= I14), D33 (= D35), V34 (≠ I36), D53 (≠ N53), C54 (≠ V54), L75 (= L75), P76 (≠ A76), A77 (= A77), M79 (≠ L79), T117 (≠ S118), Y142 (= Y143), K146 (= K147), L169 (≠ Y170), P170 (= P171), I172 (≠ L173)
5k4wC Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to nadh and l-threonine refined to 1.72 angstroms (see paper)
37% identity, 98% coverage: 4:312/314 of query aligns to 4:312/321 of 5k4wC
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G9), G12 (= G12), Q13 (= Q13), I14 (= I14), D35 (= D35), V36 (≠ I36), D55 (≠ N53), C56 (≠ V54), L77 (= L75), P78 (≠ A76), A79 (= A77), M81 (≠ L79), P117 (= P116), Y144 (= Y143), K148 (= K147), L171 (≠ Y170), I174 (≠ L173)
- binding threonine: M81 (≠ L79), S82 (= S80), T119 (≠ S118), Y144 (= Y143), G184 (= G183), A185 (≠ T184), T186 (= T185), W280 (= W280)
5lc1A L-threonine dehydrogenase from trypanosoma brucei with NAD and the inhibitor pyruvate bound. (see paper)
37% identity, 98% coverage: 4:312/314 of query aligns to 3:311/320 of 5lc1A
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), Q12 (= Q13), I13 (= I14), D34 (= D35), V35 (≠ I36), D54 (≠ N53), C55 (≠ V54), L76 (= L75), P77 (≠ A76), A78 (= A77), M80 (≠ L79), P116 (= P116), T118 (≠ S118), Y143 (= Y143), K147 (= K147), L170 (≠ Y170), I173 (≠ L173)
- binding pyruvic acid: S81 (= S80), Y143 (= Y143), G183 (= G183), T185 (= T185), W279 (= W280)
5k4qA Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to NAD+ refined to 2.3 angstroms (see paper)
37% identity, 98% coverage: 4:312/314 of query aligns to 3:311/320 of 5k4qA
- binding nicotinamide-adenine-dinucleotide: G8 (= G9), L10 (≠ N11), G11 (= G12), Q12 (= Q13), I13 (= I14), D34 (= D35), V35 (≠ I36), C55 (≠ V54), L76 (= L75), P77 (≠ A76), A78 (= A77), M80 (≠ L79), P116 (= P116), S117 (= S117), Y143 (= Y143), K147 (= K147), L170 (≠ Y170), I173 (≠ L173)
Query Sequence
>CA265_RS15335 FitnessBrowser__Pedo557:CA265_RS15335
MQEKIVVLGSNGQIGTELVTMLRKIYGDDHVVACDIRRPDYDIKNSAPFEFVNVLDKEMI
NGIFHKYRPTQVYLLAALLSATGEQNPKLAWDLNMNGLLNVLDLALEYKTAKVYWPSSIA
VFGPNSPKDNTSQYCVMDPNTVYGISKLAGERWCEYYNQKYGLDVRSIRYPGLISWKAAP
GGGTTDYAIHIFHDALKKGSYQSFLSADTELPMMYMDDAIRGTIELMDAPADQISVRSSY
NFGGVSFTPEVLAAEIRKHIPEFTLTYGANDPRQQIANSWPRSIDDNYAAKDWGWKPEFD
LSKLTADMLKNLKK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory