SitesBLAST

T49 (= T50) mutation T->F,W: Increases uptake of both methylarsenite and methylarsenate and exhibits higher sensitivity to methylarsenite.
V177 (= V178) mutation to I: Decreases methylarsenite transport activity and results in higher resistance to methylarsenite. Slight decrease in methylarsenate transport activity.; mutation to R: Increases methylarsenite transport activity, leading to lower resistance to methylarsenite. Nearly complete loss of methylarsenate transport activity.

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
33% identity, 96% coverage: 8:221/224 of query aligns to 38:251/271 of P08995

query
sites
P08995

R

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V

A

A

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E

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A

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G

T

T

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N

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N

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N

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G

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N

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D

D

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
31% identity, 97% coverage: 8:224/224 of query aligns to 49:264/298 of Q6Z2T3

query
sites
Q6Z2T3

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|
A

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A

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P

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K

A132 (= A94) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 61% coverage: 8:143/224 of query aligns to 37:184/285 of P30302

query
sites
P30302

R

R

R

A

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A

A

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A

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G

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A

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F

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D

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Y

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L

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F

Sites not aligning to the query:

223 T→C: Creates some mercury-sensitivity.

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 62% coverage: 8:146/224 of query aligns to 80:216/305 of Q9SAI4

query
sites
Q9SAI4

R

R

R

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x
A

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A

A119 (≠ T50) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.

Sites not aligning to the query:

252 V→A: No effect.

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 61% coverage: 8:143/224 of query aligns to 39:186/287 of P43286

query
sites
P43286

R

R

R

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N

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F

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P23645 Neurogenic protein big brain from Drosophila melanogaster (Fruit fly) (see 3 papers)
29% identity, 79% coverage: 43:220/224 of query aligns to 99:279/696 of P23645

query
sites
P23645

L

L

G

A

N

T

T

A

L

L

A

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G

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A

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x
D

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S

D253 (≠ A194) mutation to N: No effect on transport activity.
E274 (≠ G215) mutation to Q: No effect on transport activity.

Sites not aligning to the query:

46 modified: Phosphoserine
47 modified: Phosphothreonine
71 E→D: Partial loss of transport activity and increased sensitivity to blocking by the magnesium ion.; E→K: Loss of expression in cell membrane.; E→N: Loss of transport activity.; E→Q: Loss of transport activity.
300 modified: Phosphoserine
394 modified: Phosphoserine
576 modified: Phosphoserine

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 61% coverage: 8:143/224 of query aligns to 37:184/285 of P43287

query
sites
P43287

R

R

R

A

L

V

V

I

A

A

E

E

A

F

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G

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T

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A

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N

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W

G

T

F

A

V

H

K

A

A

M

-

F

-

G

-

E

-

P

-

I

-

L

F

Q

Q

V

S

S

S

T

Y

K

Y

L

D

R

R

S

Y

G

G

D

G

Q

A

A

N

F

S

-

L

-

A

-

D

-

G

-

Y

-

N

-

T

-

G

-

T

-

G

-

L

-

A

A

A

E

E

V

I

A

G

T

T

F

F

G

V

L

L

L

V

A

Y

T

T

I

V

L

F

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial
194 R→A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
195 D→A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
197 H→A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; H→D: Reduced water transport activity. Insensitive to cytosolic acidification.; H→K: Very low water transport activity. Insensitive to cytosolic acidification.
264 H→A: No effect.

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
30% identity, 61% coverage: 8:143/224 of query aligns to 37:186/285 of Q9XF58

query
sites
Q9XF58

R

R

R

A

L

V

V

I

A

A

E

E

A

F

L

V

G

A

T

T

A

L

L

L

L

F

L

L

A

Y

V

I

V

T

V

V

G

A

S

T

G

V

I

I

M

G

G

Y

E

K

R

H

L

Q

S

T

D

D

G

A

N

A

I

A

A

S

-

G

-

P

-

D

-

A

-

C

-

G

I

V

A

G

L

V

L

L

G

G

N

I

T

A

L

W

A

A

T

F

G

G

A

G

A

M

L

I

V

F

V

I

L

L

I

V

W

Y

V

C

F

T

G

A

P

G

I

V

S

S

G

G

A
x
G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

V

G

A

L

A

F

L

L

R

A

R

R

E

K

L

V

P

S

L

L

T

V

L

R

A

A

L

L

A

L

Y

Y

P

I

L

V

A

A

Q

Q

I

C

L

L

G

G

G

A

I

I

A

C

G

G

V

V

W

G

T

L

A

V

-

K

-

G

-

F

-

Q

-

S

-

A

-

F

H

Y

A

V

M

R

F

Y

G

G

E

G

P

G

I

A

L

N

Q

E

V

L

S

S

T

A

K

G

L

Y

R

S

S

K

G

G

G

T

D

G

Q

L

A

A

F

-

A

A

E

E

V

I

A

G

T

T

F

F

G

V

L

L

L

V

A

Y

T

T

I

V

L

F

G104 (≠ A67) mutation to W: Loss of water transport.

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
31% identity, 69% coverage: 8:161/224 of query aligns to 10:174/247 of 8ct2D

query
sites
8ct2D

R

R

R

A

L

V

V

V

A

A

E

E

A

F

L

L

G

A

T

T

A

T

L

L

L

F

L

V

A

F

V

I

V

S

V

I

G

G

S

S

G

A

I

-

M

L

G

G

E

F

R

K

L

Y

S

P

D

V

G

G

N

N

I

N

A

Q

I

T

A

A

L

V

L

Q

G

D

N

N

-

V

-

K

-

V

T

S

L

L

A

A

T

F

G

G

A

L

A

S

L

I

V

A

V

T

L

L

I

A

W

Q

V

S

F

V

G

G

P

H

I

I

S

S

G

G

A

A

H

H

F

L

N

N

P

P

A

A

V

V

T

T

L

L

V

G

A

L

L

L

R

S

R

C

E

Q

L

I

P

S

L

I

T

F

L

R

A

A

L

L

A

M

Y

Y

P

I

L

I

A

A

Q

Q

-

C

-

V

-

G

-

A

-

I

-

V

-

A

-

T

-

A

I

I

L

L

G

S

G

G

I

I

A

T

G

S

V

S

W

L

T

T

A

G

H

N

A

S

M

L

F

G

G

R

E

N

P

D

I

L

L

-

Q

-

V

-

S

A

T

D

K

G

L

V

R

N

S

S

G
|
G

G

Q

D

G

Q

L

A
x
G

F

I

A

-

E

E

V

I

A

G

T

T

F

L

G

Q

L

L

L

V

A

L

T

C

I

V

L

L

G

A

V

T

S

T

R

D

F

R

R

R

P

R

E

R

-

D

-

L

-

G

S

S

T

A

P

P

M

L

A

A

V

I

G

G

L

L

Y

S

I

V

binding cholesterol: G134 (= G124), G138 (≠ A128)

Q54WT8 Aquaporin-B from Dictyostelium discoideum (Social amoeba) (see paper)
47% identity, 24% coverage: 48:100/224 of query aligns to 84:136/294 of Q54WT8

query
sites
Q54WT8

L

L

A

S

T

T

G

G

A

F

A

A

L

A

V

V

V

A

L

L

I

I

W

Y

V

S

F

F

G

A

P

D

I

V

S

S

G

G

A

A

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

F

V

A

A

T

A

C

L

V

R

T

R

R

E

K

L

T

P
x
S

L

I

T

T

L

K

A

G

L

L

A

M

Y

Y

P

V

L

G

A

A

Q

Q

I

L

L

V

G

G

G

S

I

V

S120 (≠ P84) mutation to A: Permanently unphosphorylated state, impermeable to water in Xenopus oocytes.; mutation to D: Permanently phosphorylated, impermeable to water in Xenopus oocytes.

Sites not aligning to the query:

75 modified: carbohydrate, O-linked (GalNAc...) serine
208:212 mutation Missing: Impermeable to water in Xenopus oocytes.
208:219 Required for water permeability; mutation Missing: Permeable to water in Xenopus oocytes and liposomes, impermeable to glycerol and urea.
239 Selectivity filter
275 D→A: Impermeable to water in Xenopus oocytes.; D→P: Impermeable to water in Xenopus oocytes.

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 42% coverage: 8:102/224 of query aligns to 52:146/286 of P61837

query
sites
P61837

R

R

R

A

L

G

V

I

A

A

E

E

A

F

L

I

G

A

T

T

A

F

L

L

L

F

L

L

A

Y

V

I

V

T

V

V

G

L

S

T

G

-

I

V

M

M

G

G

E

V

R

K

L

R

S

S

D

P

G

N

N

M

I

C

A

A

-

S

I

V

A

G

L

I

L

Q

G

G

N

I

T

A

L

W

A

A

T

F

G

G

A

G

A

M

L

I

V

F

V

A

L

L

I

V

W

Y

V

C

F

T

G

A

P

G

I

I

S

S

G

G

A

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

V

G

A

L

A

F

L

L

R

A

R

R

E

K

L

L

P

S

L

L

T

T

L

R

A

A

L

L

A

Y

Y

Y

P

I

L

V

A

M

Q

Q

I

C

L

L

G

G

G

A

I

I

A

C

G

G

Sites not aligning to the query:

1 modified: N-acetylmethionine

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
28% identity, 58% coverage: 8:136/224 of query aligns to 59:186/306 of I1CR68

query
sites
I1CR68

R

R

R

E

L

F

V

L

A

A

E

E

A

F

L

I

G

G

T

T

A

V

L

I

L

L

L

-

A

-

V

V

V

L

G

T

S

C

G

G

I

F

M

C

G

A

E

E

R

Q

L

T

S

L

D

H

G

I

N

E

I

E

A

S

I

K

A

S

L

W

L

L

G

T

N

S

T

S

L

F

A

G

T

S

G

G

A

L

A

S

L

V

V

L

V

I

L

G

I

I

W

C

V

V

F

S

G

G

P

H

I

V

S

S

G

G

A

A

H

H

F

L

N

N

P

P

A

A

V

V

T

T

L

I

V

A

A

F

A

C

L

I

R

F

R

S

E

G

L

F

P

P

L

I

T

R

L

K

A

V

L

P

A

S

Y

Y

P

I

L

T

A

A

Q

Q

I

L

L

L

G

G

G

A

I

F

A

A

G

G

V

A

W

A

T

L

A

L

H

Y

A

I

M

I

F

I

G

E

E

P

P

A

I

I

L

V

Q

Q

V

F

S

D

T

G

K

G

L

Q

R

R

S

Y

G

I

-

L

G

G

D

E

Q

K

A

S

F

T

A

A

E

G

V

I

V

F

A

G

T

T

F

Y

Sites not aligning to the query:

275 H→A: Affects pH sensing; when associated with A-85.

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
34% identity, 42% coverage: 8:100/224 of query aligns to 5:95/230 of 3nkaA

query
sites
3nkaA

R

R

R

K

L

L

V

A

A

A

E

E

A

C

L

F

G

G

T

T

A

F

L

W

L

L

L

V

A

F

V

G

V

C

G

G

S

S

G
x
A

I

V

M

L

G

A

E

A

R

G

L

F

S

P

D

E

G

-

N

-

I

L

A

G

I

I

A

G

L

F

L

A

G

G

N
x
V

T

A

L

L

A

A

T

F

G

G

A

L

A

T

L

V

V

L

V

T

L

M

I

A

W

F

V

A

F

V

G

G

P

H

I

I

S

S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

V

G

A

L

A

W

L

A

R

G

E

R

L

F

P

P

L

A

T

K

L

E

A

V

L

V

A

G

Y

Y

P

V

L

I

A

A

Q

Q

I

V

L

V

G

G

I

I

binding glycerol: A25 (≠ G28), V41 (≠ N46)

Sites not aligning to the query:

binding glycerol: 180, 184

P60844 Aquaporin Z; Bacterial nodulin-like intrinsic protein; Water channel AqpZ from Escherichia coli (strain K12) (see paper)
34% identity, 42% coverage: 8:100/224 of query aligns to 3:93/231 of P60844

query
sites
P60844

R

R

R

K

L

L

V

A

A

A

E

E

A
x
C

L

F

G

G

T

T

A

F

L

W

L

L

L

V

A

F

V

G

V
x
C

G

G

S

S

G

A

I

V

M

L

G

A

E

A

R

G

L

F

S

P

D

E

G

-

N

-

I

L

A

G

I

I

A

G

L

F

L

A

G

G

N

V

T

A

L

L

A

A

T

F

G

G

A

L

A

T

L

V

V

L

V

T

L

M

I

A

W

F

V

A

F

V

G

G

P

H

I

I

S

S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

V

G

A

L

A

W

L

A

R

G

E

R

L

F

P

P

L

A

T

K

L

E

A

V

L

V

A

G

Y

Y

P

V

L

I

A

A

Q

Q

I

V

L

V

G

G

I

I

C9 (≠ A14) mutation to S: No effect.
C20 (≠ V25) mutation to S: Loss of oligomerization; no alteration of water permeability.

Sites not aligning to the query:

183 T→C: No effect.
189 mutation R->V,S: Loss of function.

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 42% coverage: 8:102/224 of query aligns to 52:146/286 of Q06611

query
sites
Q06611

R

R

R

A

L

G

V

I

A

A

E

E

A

F

L

I

G

A

T

T

A

F

L

L

L

F

L

L

A

Y

V

I

V

T

V

V

G

L

S

T

G

-

I

V

M

M

G

G

E

V

R

K

L

R

S

S

D

P

G

N

N

M

I

C

A

A

-

S

I

V

A

G

L

I

L

Q

G

G

N

I

T

A

L

W

A

A

T

F

G

G

A

G

A

M

L

I

V

F

V

A

L

L

I

V

W

Y

V

C

F

T

G

A

P

G

I

I

S

S

G

G

A

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

V

G

A

L

A

F

L

L

R

A

R

R

E

K

L

L

P

S

L

L

T

T

L

R

A

A

L

V

A

Y

Y

Y

P

I

L

V

A

M

Q

Q

I

C

L

L

G

G

G

A

I

I

A

C

G

G

Sites not aligning to the query:

1 modified: N-acetylmethionine

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
34% identity, 42% coverage: 8:100/224 of query aligns to 5:95/232 of 2o9eA

query
sites
2o9eA

R

R

R

K

L

L

V

A

A

A

E

E

A

S

L

F

G

G

T

T

A

F

L

W

L

L

L

V

A

F

V

G

V

S

G

G

S

S

G

A

I

V

M

L

G

A

E

A

R

G

L

F

S

P

D

E

G

-

N

-

I

L

A

G

I

I

A

G

L

F

L

A

G

G

N

V

T

A

L

L

A

A

T

F

G

G

A

L

A

T

L

V

V

L

V

T

L

M

I

A

W

F

V

A

F

V

G

G

P

H

I

I

S

S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

T

T

L

I

V

G

A

L

A

W

L

A

R

G

E

R

L

F

P

P

L

A

T

K

L

E

A

V

L

V

A

G

Y

Y

P

V

L

I

A

A

Q

Q

I

V

L

V

G

G

I

I

Sites not aligning to the query:

binding mercury (ii) ion: 140, 179

Q39196 Probable aquaporin PIP1-4; Plasma membrane intrinsic protein 1-4; AtPIP1;4; Transmembrane protein C; TMP-C from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 42% coverage: 8:102/224 of query aligns to 53:147/287 of Q39196

query
sites
Q39196

R

R

R

A

L

G

V

I

A

A

E

E

A

F

L

I

G

A

T

T

A

F

L

L

L

F

L

L

A

Y

V

I

V

T

V

V

G

L

S

T

G

V

I

M

M

G

G

V

E

K

R

R

L

A

S

P

D

N

G

M

N

C

I

A

A

S

I

V

A

G

L

I

L

Q

G

G

N

I

T

A

L

W

A

A

T

F

G

G

A

G

A

M

L

I

V

F

V

A

L

L

I

V

W

Y

V

C

F

T

G

A

P

G

I

I

S

S

G

G

A

G

H

H

F

I

N

N

P

P

A

A

V

V

T

T

L

F

V

G

A

L

A

F

L

L

R

A

R

R

E

K

L

L

P

S

L

L

T

T

L

R

A

A

L

V

A

F

Y

Y

P

M

L

I

A

M

Q

Q

I

C

L

L

G

G

G

A

I

I

A

C

G

G

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 64% coverage: 1:143/224 of query aligns to 45:193/286 of Q08733

query
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Q08733

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Sites not aligning to the query:

1 modified: N-acetylmethionine

O94778 Aquaporin-8; AQP-8 from Homo sapiens (Human) (see 4 papers)
42% identity, 40% coverage: 13:102/224 of query aligns to 41:124/261 of O94778

query
sites
O94778

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F48 (≠ L20) mutation to A: Loss of hydrogen peroxide transport activity under stress condition.
C53 (≠ S27) modified: Cysteine persulfide; modified: Cysteine sulfenic acid (-SOH); mutation to S: Does not affect hydrogen peroxide transport under stress condition.
H72 (≠ T50) mutation to A: Does not affect hydrogen peroxide transport activity under stress condition.

Sites not aligning to the query:

8 C→S: Does not affect loss of hydrogen peroxide transport after stress.
38 C→S: Does not affect loss of hydrogen peroxide transport after stress.
173 C→A: Loss of hydrogen peroxide transporter activity.; C→S: Slightly affect hydrogen peroxide transport after stress.
208 C→S: Does not affect loss of hydrogen peroxide transport after stress.
213 R→A: Does not affect hydrogen peroxide transport activity under stress condition.
229 I → M: in a breast cancer sample; somatic mutation
247 C→S: Does not affect loss of hydrogen peroxide transport after stress.

Query Sequence

>CCNA_01572 FitnessBrowser__Caulo:CCNA_01572
MSPFDTPRRLVAEALGTALLLAVVVGSGIMGERLSDGNIAIALLGNTLATGAALVVLIWV
FGPISGAHFNPAVTLVAALRRELPLTLALAYPLAQILGGIAGVWTAHAMFGEPILQVSTK
LRSGGDQAFAEVVATFGLLATILGVSRFRPESTPMAVGLYITAAYWFTASTSFANPAVTV
ARSLSDSFAGIAPASAPAFILAQLVGALSAAALFGWLLKTDTSR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory